Cmc12g0327391 (gene) Melon (Charmono) v1.1

BLAST of Cmc12g0327391 vs. NCBI nr
Match: XP_008445757.1 (PREDICTED: cannabidiolic acid synthase-like 2 [Cucumis melo] >KAA0047085.1 cannabidiolic acid synthase-like 2 [Cucumis melo var. makuwa] >TYK05070.1 cannabidiolic acid synthase-like 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
           MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS
Sbjct: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60

Query: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF
Sbjct: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120

Query: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
           EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC
Sbjct: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
           PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG
Sbjct: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240

Query: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
           CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG
Sbjct: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300

Query: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360
           KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF
Sbjct: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360

Query: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420
           QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG
Sbjct: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420

Query: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480
           KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA
Sbjct: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480

Query: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540
           AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT
Sbjct: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540

Query: 541 LSGISEESFSASL 554
           LSGISEESFSASL
Sbjct: 541 LSGISEESFSASL 553

BLAST of Cmc12g0327391 vs. NCBI nr
Match: XP_004135742.2 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653322.1 hypothetical protein Csa_023372 [Cucumis sativus])

HSP 1 Score: 1049.7 bits (2713), Expect = 9.1e-303
Identity = 520/556 (93.53%), Postives = 538/556 (96.76%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAF--IIVVVLSTATTSFC-TQECDSFVQCLFDYGSPNSNPIANVIY 60
           MKCFPIL APFGLIAF  IIVVVLSTAT SFC TQE +SFVQCLFDYGS ++NPIAN+IY
Sbjct: 1   MKCFPILPAPFGLIAFIIIIVVVLSTATPSFCSTQESESFVQCLFDYGSSSTNPIANLIY 60

Query: 61  TPSNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGG 120
           TP+NSSYSSILNFSIQNLRFLNK+I KP AIITP HVSHIQAAVICSKSHGFQIRTRSGG
Sbjct: 61  TPTNSSYSSILNFSIQNLRFLNKDIPKPFAIITPLHVSHIQAAVICSKSHGFQIRTRSGG 120

Query: 121 HDFEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPA 180
           HDFEGLSYIS +PFV+VDLINL SISIDVENQNAWV SGATLGELYYRI+EKSQTLAFPA
Sbjct: 121 HDFEGLSYISDIPFVLVDLINLKSISIDVENQNAWVHSGATLGELYYRISEKSQTLAFPA 180

Query: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240
           GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG
Sbjct: 181 GSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGG 240

Query: 241 GGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300
           GGG FGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL
Sbjct: 241 GGGSFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL 300

Query: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLM 360
           TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQL LTKEDCLEMSWIQSVLLM
Sbjct: 301 TGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLGLTKEDCLEMSWIQSVLLM 360

Query: 361 GWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVP 420
           GWFQK DPLEVLLNR+RLYSEISKIKSDYVKEHIP+VA+KGMWERLKS+DVELSQIMFVP
Sbjct: 361 GWFQKEDPLEVLLNRSRLYSEISKIKSDYVKEHIPMVAVKGMWERLKSQDVELSQIMFVP 420

Query: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKS 480
           YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAE+ HLNWIREIYDYMTPFVSKS
Sbjct: 421 YGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEKSHLNWIREIYDYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQS 540
           PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFD+LV+VKTKVDPSDFFRH+QS
Sbjct: 481 PRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDRLVHVKTKVDPSDFFRHEQS 540

Query: 541 IPTLSGISEESFSASL 554
           IPTLSGIS+E FSASL
Sbjct: 541 IPTLSGISKELFSASL 556

BLAST of Cmc12g0327391 vs. NCBI nr
Match: XP_038895846.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 958.0 bits (2475), Expect = 3.6e-275
Identity = 462/551 (83.85%), Postives = 508/551 (92.20%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
           M+CFP L AP G IAFII +VL+T TTSFCTQE DSFVQCLFDYGS N++PI++VIYTP 
Sbjct: 1   MECFP-LTAPLGRIAFIIFIVLTTTTTSFCTQESDSFVQCLFDYGSLNTHPISDVIYTPI 60

Query: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           NSSYSS+LNFS+QNLRFL K   KPLAIITPSHVSHIQAAVICSKSHG QIRTRSGGHDF
Sbjct: 61  NSSYSSVLNFSVQNLRFLTKKTPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDF 120

Query: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
           EGLSYIS  PF++VDLINL SI+IDVEN+NAWV+SGATLGELYYRIAEKSQTLAFPAGSC
Sbjct: 121 EGLSYISDAPFIVVDLINLRSIAIDVENENAWVQSGATLGELYYRIAEKSQTLAFPAGSC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
            TVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGK+H++ESMGEDLFWAIRGGGGG
Sbjct: 181 STVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKLHDRESMGEDLFWAIRGGGGG 240

Query: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
            FGIVV+WKVKLV VPA VT+CEANRTLEED ++KLIHEWQYVAPKLDANLLLGISLTGG
Sbjct: 241 SFGIVVSWKVKLVRVPATVTVCEANRTLEED-VMKLIHEWQYVAPKLDANLLLGISLTGG 300

Query: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360
           KVTEDEEGKINPIASF   FLGKAEE++TILKPTF QL L +EDCLE+SWI+SVLLMGWF
Sbjct: 301 KVTEDEEGKINPIASFVAQFLGKAEELMTILKPTFSQLGLKREDCLEISWIESVLLMGWF 360

Query: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420
           QK DP+EVLLNRTRLYSEISKIKSDYVKE IP  ++KGMWERLKS+DVEL+QIMFVPYGG
Sbjct: 361 QKEDPIEVLLNRTRLYSEISKIKSDYVKERIPKASIKGMWERLKSQDVELAQIMFVPYGG 420

Query: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480
           +MSEV + ETP+SHR DY YL+GYIAKWKNG+VEAE+RHLNWIREIYDYMTPFVS SPR 
Sbjct: 421 RMSEVYEWETPYSHRGDYMYLIGYIAKWKNGTVEAEKRHLNWIREIYDYMTPFVSNSPRV 480

Query: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540
           AYVNYRDLDIGTNNKY +TSYKRAR WG+KYFG NFD+LVY+KTKVDPS+FF+H+QSIP 
Sbjct: 481 AYVNYRDLDIGTNNKYDKTSYKRARNWGMKYFGNNFDRLVYIKTKVDPSNFFKHEQSIPI 540

Query: 541 LSGISEESFSA 552
           L+GI+EE FSA
Sbjct: 541 LTGITEEFFSA 549

BLAST of Cmc12g0327391 vs. NCBI nr
Match: XP_038895208.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 763.5 bits (1970), Expect = 1.3e-216
Identity = 382/544 (70.22%), Postives = 446/544 (81.99%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
           MK F  L  PF L    IV+ +S +   F T++ ++FVQCL +  S ++ PI+N+IYTP 
Sbjct: 1   MKSF--LLTPFALT--FIVLSISPSWVVFSTKKHENFVQCLLN-NSLSTYPISNLIYTPI 60

Query: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           NSSYSSILNFSIQNLRF +K   KPLAIITPSHVSHIQAA+ICSKSH  QIRTRSGGHDF
Sbjct: 61  NSSYSSILNFSIQNLRFSSKETPKPLAIITPSHVSHIQAAIICSKSHALQIRTRSGGHDF 120

Query: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
           EGLSYIS VPFV+VDLINL SI+IDVEN+ AWV+SGATLGELYYRIAEKS+TLAFPAGSC
Sbjct: 121 EGLSYISDVPFVVVDLINLKSIAIDVENETAWVQSGATLGELYYRIAEKSRTLAFPAGSC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
           PTVGVGGHLSGGG+GWL+RKYGLAAD+VIDA  +DANGKV+++ESMGEDLFWAIRGGGGG
Sbjct: 181 PTVGVGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGG 240

Query: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
            FGIVVAWKVKLV VPA VTIC  NR LEE+  +KLIH WQYV  KLD NLLLGISL GG
Sbjct: 241 SFGIVVAWKVKLVRVPATVTICGINRNLEEEDTIKLIHRWQYVTNKLDENLLLGISLIGG 300

Query: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360
             T+ E GK NP+A FS  FLGK  E++TIL   F +L L KEDC EMSWI+SVL+M  F
Sbjct: 301 NSTQ-EGGKTNPMALFSSFFLGKVNELVTILNTNFSELGLAKEDCSEMSWIKSVLIMAGF 360

Query: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420
            K + LEVLLNR       +KIKSDYVKE I   + K M++RLK++D+E++QIMF+PYGG
Sbjct: 361 PKQEALEVLLNRAPPSGLSTKIKSDYVKEPISKASFKTMFKRLKAEDIEVAQIMFIPYGG 420

Query: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480
           +MSE+ +S TPF HR+   Y +GY  KWK  S++AE+RHLNW+R IYDYMTPFVSKSPRA
Sbjct: 421 RMSEISESLTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWVRVIYDYMTPFVSKSPRA 480

Query: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540
           AY NYRDLDIGTNNK G+TSY RARVWGLKYFGKNFD+LV+VKTK+DP++FFR++QSIPT
Sbjct: 481 AYNNYRDLDIGTNNKCGKTSYNRARVWGLKYFGKNFDRLVHVKTKIDPTNFFRNEQSIPT 538

Query: 541 LSGI 545
           L+ I
Sbjct: 541 LTDI 538

BLAST of Cmc12g0327391 vs. NCBI nr
Match: XP_011648520.1 (cannabidiolic acid synthase [Cucumis sativus] >KAE8653301.1 hypothetical protein Csa_023300 [Cucumis sativus])

HSP 1 Score: 743.8 bits (1919), Expect = 1.1e-210
Identity = 358/544 (65.81%), Postives = 436/544 (80.15%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
           MK F     PF L  F IV+   + + +  T+  ++F+QCL +     +  I+N+IYTP 
Sbjct: 1   MKSF--FLTPFAL--FFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPI 60

Query: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           NSS+ S+LNFSIQNLRF  K   KPLAIITPSHVSHIQA ++CSKSH  QIR RSGGHDF
Sbjct: 61  NSSFYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDF 120

Query: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
           EGLSY+S VPF++VDLINL SI+IDVEN+NAWV+SGATLGE YYRI EKSQTLAFPAGSC
Sbjct: 121 EGLSYVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
           PTVG+GGHLSGGG+GWL+RKYGLAAD+VIDA  VDANGKV+++ESMG+DLFWAIRGGGGG
Sbjct: 181 PTVGIGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGG 240

Query: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
            FGI+VAWKVKLV VPA VTIC + R+LEE+  +KLIH+WQY+  KLD NLLLGISLTGG
Sbjct: 241 SFGIIVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGG 300

Query: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360
             T+ E GKINP A FS  FLGK  E++ IL   FP+L+L+KE+C EMSWI++VL M  F
Sbjct: 301 NSTQ-ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGF 360

Query: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420
              +P EVLLNRT  +   +KIKSDY+K+ +   A K M +RLK++D+E++QIMF+PYGG
Sbjct: 361 PNQEPFEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGG 420

Query: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480
           +MSE+ +SE PF HR+   Y +GY  KWK+ S++AE+RHLNWIR+IYDYMTPFVSKSPRA
Sbjct: 421 RMSEISESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRA 480

Query: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540
            Y NYRDLDIG NNKYG+ +Y  ARVWG KYFGKNFD+LV++KTK+DP+DFFR++QSIP 
Sbjct: 481 TYCNYRDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPA 539

Query: 541 LSGI 545
           L  I
Sbjct: 541 LKNI 539

BLAST of Cmc12g0327391 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.4e-143
Identity = 258/546 (47.25%), Postives = 362/546 (66.30%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
           MKC       F  +  II   LS           ++F++CL  Y  P +   A ++YT  
Sbjct: 1   MKCSTFC---FWYVCKIIFFFLSFNIQISIANPQENFLKCLSQY-IPTNVTNAKLVYTQH 60

Query: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           +  Y SILN ++QNLRF +    KPL I TP +VSHIQ  ++CSK  G QIRTRSGGHD 
Sbjct: 61  DQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVSHIQGTILCSKKVGLQIRTRSGGHDA 120

Query: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
           EG+SYIS VPFV+VDL N++S+ IDV +Q AWVESGATLGE+YY I E ++ L+FPAG C
Sbjct: 121 EGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVESGATLGEVYYWINENNENLSFPAGYC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
           PTVG GGH SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG 
Sbjct: 181 PTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGE 240

Query: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
            FGI+ AWK++LV VP+  TI    + +E   ++KL+++WQ +A   +  LLL       
Sbjct: 241 NFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITR 300

Query: 301 KVTEDE-EGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVL---- 360
            +T+++ + K    + FS +F G  + ++ ++  +FP+L + K DC ++SWI +++    
Sbjct: 301 NITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSG 360

Query: 361 LMGWFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMF 420
           ++ +       E+LL+R+        IK DYVK+ IP  A+  + E+L  +DV +   +F
Sbjct: 361 VVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVGMFVF 420

Query: 421 VPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVS 480
            PYGG M E+ +S  PF HR+  +Y + YIA W+    E  E+H+NWIR +Y++ TP+VS
Sbjct: 421 YPYGGIMDEISESAIPFPHRAGITYEIWYIASWE--KQEDNEKHINWIRNVYNFTTPYVS 480

Query: 481 KSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQ 540
           ++PR AY+NYRDLD+G  N     +Y +AR+WG KYFGKNF++LV VKTKVDP +FFR++
Sbjct: 481 QNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNE 540

Query: 541 QSIPTL 542
           QSIP L
Sbjct: 541 QSIPPL 540

BLAST of Cmc12g0327391 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.4e-143
Identity = 258/539 (47.87%), Postives = 358/539 (66.42%), Query Frame = 0

Query: 8   AAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPSNSSYSSI 67
           A  F  +  II   LS           ++F++C F    PN+     ++YT  +  Y SI
Sbjct: 5   AFSFWFVCKIIFFFLSFHIQISIANPRENFLKC-FSKHIPNNVANPKLVYTQHDQLYMSI 64

Query: 68  LNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS 127
           LN +IQNLRF++    KPL I+TPS+ SHIQA ++CSK  G QIRTRSGGHD EG+SYIS
Sbjct: 65  LNSTIQNLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYIS 124

Query: 128 GVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSCPTVGVGG 187
            VPFV+VDL N++SI IDV +Q AWVE+GATLGE+YY I EK++ L+FP G CPTVGVGG
Sbjct: 125 QVPFVVVDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGG 184

Query: 188 HLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGCFGIVVA 247
           H SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG  FGI+ A
Sbjct: 185 HFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAA 244

Query: 248 WKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE- 307
           WK+KLV VP++ TI    + +E   ++KL ++WQ +A K D +L+L        +T++  
Sbjct: 245 WKIKLVAVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHG 304

Query: 308 EGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVL----LMGWFQK 367
           + K      FS +F G  + ++ ++  +FP+L + K DC E SWI + +    ++ +   
Sbjct: 305 KNKTTVHGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTA 364

Query: 368 GDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGGKM 427
               E+LL+R+        IK DYVK+ IP  A+  + E+L  +DV     +  PYGG M
Sbjct: 365 NFKKEILLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIM 424

Query: 428 SEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRAAY 487
            E+ +S  PF HR+   Y + Y A W+    E  E+H+NW+R +Y++ TP+VS++PR AY
Sbjct: 425 EEISESAIPFPHRAGIMYELWYTASWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAY 484

Query: 488 VNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPTL 542
           +NYRDLD+G  N     +Y +AR+WG KYFGKNF++LV VKTKVDP++FFR++QSIP L
Sbjct: 485 LNYRDLDLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Cmc12g0327391 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 7.5e-143
Identity = 260/550 (47.27%), Postives = 362/550 (65.82%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
           MKC       F  +  II   LS           ++F++C   Y  P +   A ++YT  
Sbjct: 1   MKCSTFC---FWYVCKIIFFFLSFNIQISIANPQENFLKCFSQY-IPTNVTNAKLVYTQH 60

Query: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           +  Y SILN +IQNLRF +    KPL IITP +VSHIQ  ++CSK  G QIRTRSGGHD 
Sbjct: 61  DQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVSHIQGTILCSKKVGLQIRTRSGGHDA 120

Query: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
           EG+SYIS VPFV+VDL N++S+ IDV +Q AWVE+GATLGE+YY I E ++ L+FPAG C
Sbjct: 121 EGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEAGATLGEVYYWINENNENLSFPAGYC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
           PTVG GGH SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG 
Sbjct: 181 PTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGE 240

Query: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
            FGI+ AWK++LV VP+  TI    + +E   ++KL+++WQ +A   +  LLL       
Sbjct: 241 NFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVKLVNKWQNIAYMYEKELLLFTHFITR 300

Query: 301 KVTEDE-EGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMG- 360
            +T+++ + K    + FS +F G  + ++ ++  +FP+L + K DC ++SWI +++    
Sbjct: 301 NITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKQLSWIDTIIFYSG 360

Query: 361 -------WFQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELS 420
                  +F+K    E+LL+R+        IK DYVK+ IP  A+  + E+L  +DV + 
Sbjct: 361 VVNYNTTYFKK----EILLDRSGGRKAAFSIKLDYVKKPIPETAMVTILEKLYEEDVGVG 420

Query: 421 QIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMT 480
             +F PYGG M E+ +S  PF HR+   Y + YIA W+    E  E+H+NWIR +Y++ T
Sbjct: 421 MFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWE--KQEDNEKHINWIRNVYNFTT 480

Query: 481 PFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDF 540
           P+VS++PR AY+NYRDLD+G  N     +Y +AR+WG KYFGKNF++LV VKTKVDP +F
Sbjct: 481 PYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNF 540

Query: 541 FRHQQSIPTL 542
           FR++QSIP L
Sbjct: 541 FRNEQSIPPL 540

BLAST of Cmc12g0327391 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 9.9e-143
Identity = 252/539 (46.75%), Postives = 358/539 (66.42%), Query Frame = 0

Query: 8   AAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPSNSSYSSI 67
           A  F  +  II   LS           ++F++C  +Y  PN+      IYT  +  Y S+
Sbjct: 5   AFSFWFVCKIIFFFLSFNIQISIANPQENFLKCFSEY-IPNNPANPKFIYTQHDQLYMSV 64

Query: 68  LNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS 127
           LN +IQNLRF +    KPL I+TPS+VSHIQA+++CSK  G QIRTRSGGHD EGLSYIS
Sbjct: 65  LNSTIQNLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYIS 124

Query: 128 GVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSCPTVGVGG 187
            VPF +VDL N++++ +D+ +Q AWVE+GATLGE+YY I E ++  +FP G CPTVGVGG
Sbjct: 125 QVPFAIVDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGG 184

Query: 188 HLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGCFGIVVA 247
           H SGGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWAIRGGGG  FGI+ A
Sbjct: 185 HFSGGGYGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAA 244

Query: 248 WKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDE- 307
           WK+KLV VP++ TI    + +E   ++KL ++WQ +A K D +L+L        +T++  
Sbjct: 245 WKIKLVVVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHG 304

Query: 308 EGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVL----LMGWFQK 367
           + K      FS +FLG  + ++ ++  +FP+L + K DC E+SWI + +    ++ +   
Sbjct: 305 KNKTTVHGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTA 364

Query: 368 GDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGGKM 427
               E+LL+R+        IK DYVK+ IP  A+  + E+L  ++V +   +  PYGG M
Sbjct: 365 NFKKEILLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIM 424

Query: 428 SEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRAAY 487
            E+ +S  PF HR+   Y + Y A W+    E  E+H+NW+R +Y++ TP+VS++PR AY
Sbjct: 425 DEISESAIPFPHRAGIMYELWYTATWE--KQEDNEKHINWVRSVYNFTTPYVSQNPRLAY 484

Query: 488 VNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPTL 542
           +NYRDLD+G  N     +Y +AR+WG KYFGKNF++LV VKTK DP++FFR++QSIP L
Sbjct: 485 LNYRDLDLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of Cmc12g0327391 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.9e-141
Identity = 258/546 (47.25%), Postives = 356/546 (65.20%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
           MKC       F  +  II    S    +      ++F++C   Y  PN+     ++YT +
Sbjct: 1   MKCSTF---SFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQY-IPNNATNLKLVYTQN 60

Query: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           N  Y S+LN +I NLRF +    KPL I+TPSHVSHIQ  ++CSK  G QIRTRSGGHD 
Sbjct: 61  NPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDS 120

Query: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
           EG+SYIS VPFV+VDL N+ SI IDV +Q AWVE+GATLGE+YY + EK++ L+  AG C
Sbjct: 121 EGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
           PTV  GGH  GGGYG L+R YGLAAD++IDA+LV+ +GKV +++SMGEDLFWA+RGGG  
Sbjct: 181 PTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAE 240

Query: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
            FGI+VAWK++LV VP + T+    + +E   ++KL+++WQ +A K D +LLL       
Sbjct: 241 SFGIIVAWKIRLVAVP-KSTMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITR 300

Query: 301 KVTEDE-EGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGW 360
            +T+++ + K      FS +FLG  + ++ ++  +FP+L + K DC ++SWI +++    
Sbjct: 301 NITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSG 360

Query: 361 FQKGDP----LEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMF 420
               D      E+LL+R+   +   KIK DYVK+ IP      + E+L  +D+       
Sbjct: 361 VVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYAL 420

Query: 421 VPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVS 480
            PYGG M E+ +S  PF HR+   Y + YI  W+    E  E+HLNWIR IY++MTP+VS
Sbjct: 421 YPYGGIMDEISESAIPFPHRAGILYELWYICSWE--KQEDNEKHLNWIRNIYNFMTPYVS 480

Query: 481 KSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQ 540
           K+PR AY+NYRDLDIG N+     +Y +AR+WG KYFGKNFD+LV VKT VDP++FFR++
Sbjct: 481 KNPRLAYLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNE 539

Query: 541 QSIPTL 542
           QSIP L
Sbjct: 541 QSIPPL 539

BLAST of Cmc12g0327391 vs. ExPASy TrEMBL
Match: A0A5D3C112 (Cannabidiolic acid synthase-like 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00400 PE=3 SV=1)

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
           MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS
Sbjct: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60

Query: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF
Sbjct: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120

Query: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
           EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC
Sbjct: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
           PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG
Sbjct: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240

Query: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
           CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG
Sbjct: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300

Query: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360
           KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF
Sbjct: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360

Query: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420
           QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG
Sbjct: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420

Query: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480
           KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA
Sbjct: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480

Query: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540
           AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT
Sbjct: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540

Query: 541 LSGISEESFSASL 554
           LSGISEESFSASL
Sbjct: 541 LSGISEESFSASL 553

BLAST of Cmc12g0327391 vs. ExPASy TrEMBL
Match: A0A1S3BEA9 (cannabidiolic acid synthase-like 2 OS=Cucumis melo OX=3656 GN=LOC103488685 PE=3 SV=1)

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 553/553 (100.00%), Postives = 553/553 (100.00%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
           MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS
Sbjct: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60

Query: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF
Sbjct: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120

Query: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
           EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC
Sbjct: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180

Query: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
           PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG
Sbjct: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240

Query: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
           CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG
Sbjct: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300

Query: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360
           KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF
Sbjct: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360

Query: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420
           QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG
Sbjct: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420

Query: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480
           KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA
Sbjct: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480

Query: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540
           AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT
Sbjct: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540

Query: 541 LSGISEESFSASL 554
           LSGISEESFSASL
Sbjct: 541 LSGISEESFSASL 553

BLAST of Cmc12g0327391 vs. ExPASy TrEMBL
Match: A0A1S3BE36 (cannabidiolic acid synthase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488617 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 1.1e-210
Identity = 359/544 (65.99%), Postives = 438/544 (80.51%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPS 60
           MK F  L  PF L  F IV+   + + +  T++ ++F+QCLF+     +  I+N+IYTP 
Sbjct: 3   MKSF--LLTPFAL--FFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPI 62

Query: 61  NSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDF 120
           NSS+ S+LNFSIQNLRF  K   KPLAIITPSHVSHIQAA+ICSKSH  QIR RSGGHDF
Sbjct: 63  NSSFYSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDF 122

Query: 121 EGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 180
           EGLSY+S VPF++VDLINL SI+IDVE +NAWV+SGATLGE YYRI EKS+TLAFPAGSC
Sbjct: 123 EGLSYVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSC 182

Query: 181 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 240
           PTVG+GGHLSGGG+GWL+RKYGLAAD+VIDA  +DANGKV+++ESMGEDLFWAIRGGGGG
Sbjct: 183 PTVGIGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGG 242

Query: 241 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 300
            FGI+VAWKVKLV VPA VTIC + R+LEE+  +KLIH+WQY+   LD NLLLGISLTGG
Sbjct: 243 SFGIIVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGG 302

Query: 301 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 360
            +T+ E GK NP A FS  FLGK  E++ IL   FP+L+L+KE+C EMSWI++VL+M  F
Sbjct: 303 NLTQ-ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGF 362

Query: 361 QKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGG 420
              +P EVLLNRT L+   +KIKSDY+K+ +   A K M +RLK++D+E++QIMF+PYGG
Sbjct: 363 PTHEPFEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGG 422

Query: 421 KMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRA 480
           +MSE+  SETPF HR    Y +GY  KWK+ S++AE+RHL+WIR+IYDYMTPFVSKSPRA
Sbjct: 423 RMSEISKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRA 482

Query: 481 AYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 540
            Y NYRDLDIG NNKYG+ +Y  ARVWG KYFGKNFD+LV+VKT +DP+DFFR++QSIP 
Sbjct: 483 TYSNYRDLDIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPA 541

Query: 541 LSGI 545
           L  I
Sbjct: 543 LIDI 541

BLAST of Cmc12g0327391 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.8e-208
Identity = 356/543 (65.56%), Postives = 435/543 (80.11%), Query Frame = 0

Query: 1   MKCFPILAAPFGLIAFIIVVVLSTAT-TSFCTQECDSFVQCLFDYGSPNSNPIANVIYTP 60
           MK FP+    F   AF   +VLS++   +    + + F+QCL  + SP++N I+  IYTP
Sbjct: 1   MKSFPLNPLAF---AFACSIVLSSSLFWAASAHKHEGFLQCLTRH-SPSNNSISKFIYTP 60

Query: 61  SNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHD 120
           +N SYSSILNFSIQNLRF      KPL +I PSH+SHIQAAVICSK+HG QIRTRSGGHD
Sbjct: 61  ANFSYSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHD 120

Query: 121 FEGLSYISGVPFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGS 180
           FEGLSY+S +PF+++DLI+L SI+ID +N  AWV+SGATLGELYYRIA+KSQTLAFPAGS
Sbjct: 121 FEGLSYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGS 180

Query: 181 CPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGG 240
           CPTVGVGGHLSGGGYG+LIRKYGLAADHVIDA+LVDANG++ N+ESM +DLFW IRGGGG
Sbjct: 181 CPTVGVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGG 240

Query: 241 GCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTG 300
           G FGIVVAWK++LV VP  +T C  NRTL+ D + KLIH WQYVA KL+ NLLLGISLTG
Sbjct: 241 GSFGIVVAWKLRLVPVPTTLTTCTVNRTLDRDGV-KLIHRWQYVAHKLEDNLLLGISLTG 300

Query: 301 GKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGW 360
           G +   + GK NP+ASF   FLGK +E++ I+  TFP+L LTKEDC+E SWI+S L+   
Sbjct: 301 GHILTQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAG 360

Query: 361 FQKGDPLEVLLNRTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYG 420
           F  G+ LE LL R  L +  +KIKSDYVKE I     KG+WERL ++D+EL+Q+MFVPYG
Sbjct: 361 FSAGESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYG 420

Query: 421 GKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPR 480
           G+MSE  +SETP+ HR++  Y +GYI KW+  S +AE+RHLNW+REIYDYM P+V+KSPR
Sbjct: 421 GRMSEFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPR 480

Query: 481 AAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIP 540
           AAYVNYRDLDIG NN+Y +T+Y+RA+VWGLKYFGKNF++LV VKT+VDPSDFFRH+QSIP
Sbjct: 481 AAYVNYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIP 538

Query: 541 TLS 543
           T S
Sbjct: 541 TFS 538

BLAST of Cmc12g0327391 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 731.1 bits (1886), Expect = 3.5e-207
Identity = 357/530 (67.36%), Postives = 431/530 (81.32%), Query Frame = 0

Query: 14  IAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSN-PIANVIYTPSNSSYSSILNFSI 73
           +AFI +   S   ++F  +  +SF+QCL D+    SN  I+ VI+TP NSSYSS+L+FSI
Sbjct: 12  LAFIFLSSSSLWASAFPNKH-ESFLQCLLDHSLEYSNYSISKVIHTPINSSYSSVLDFSI 71

Query: 74  QNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISGVPFV 133
           +NLRF      KPL I+TPSHVSHIQAAVICSKS+GFQIRTRSGGHDFEGLSY++ +PF+
Sbjct: 72  RNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAHLPFI 131

Query: 134 MVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSCPTVGVGGHLSGG 193
           +VDLINL SISID+++  AWV+SGATLGELYY IA+KS+TLAFPAG CPTVGVGGH SGG
Sbjct: 132 IVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGHFSGG 191

Query: 194 GYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGCFGIVVAWKVKL 253
           GYG L+RKYGLAAD+VIDAYLVDA+GK HN+ESMGEDLFWAIRGGGGG FGIVVAWKVKL
Sbjct: 192 GYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKL 251

Query: 254 VEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINP 313
           V VPA VT+C ANRTLEE  + K+ H WQYVA KLD NL LGI   GGK+T  E GK NP
Sbjct: 252 VPVPATVTLCSANRTLEEGAV-KITHRWQYVANKLDENLFLGILWLGGKITSQEGGKTNP 311

Query: 314 IASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMG-WFQKGDPLEVLLN 373
           +A+F  +FLGKA+E+L+IL   FP+L L K+DC+E SWI+S +LMG  F     LE LL+
Sbjct: 312 VATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTLEALLS 371

Query: 374 RTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGGKMSEVDDSETP 433
           RT L +  SKIKSDYVKE +    ++G+W+RL S+D+E   I+FVPYGG+MS++ +SETP
Sbjct: 372 RTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISESETP 431

Query: 434 FSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIG 493
           FSHR+ Y Y +GYIA W+   ++AE+RHL WIRE+YDYMTPFVSKSPRAAY NYRDLDIG
Sbjct: 432 FSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYRDLDIG 491

Query: 494 TNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPTL 542
           +NNK+G+TSYK+A VWGLKYFG NF++LV+VKTKVDP DFFRH+QSIP L
Sbjct: 492 SNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of Cmc12g0327391 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 503.4 bits (1295), Expect = 2.3e-142
Identity = 247/506 (48.81%), Postives = 349/506 (68.97%), Query Frame = 0

Query: 36  SFVQCLFDYGSPNSNPIANVIYTPSNSSYSSILNFSIQNLRFLNKNISKPLAIITPSHVS 95
           SF+QCL      +SN ++ VI+TP+++S+SS+L  SIQN RF   ++ KP+ I+TP   S
Sbjct: 36  SFLQCL-SLQLNDSNIVSKVIHTPNDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPS 95

Query: 96  HIQAAVICSKSHGFQIRTRSGGHDFEGLSYISGVPFVMVDLINLNSISIDVENQNAWVES 155
            +Q+AV C++  G  IRTRSGGHD+EGLSY++  PFV++DL NL SI++DV+N++ WV++
Sbjct: 96  DVQSAVKCARRFGIHIRTRSGGHDYEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQT 155

Query: 156 GATLGELYYRIAEKSQTLAFPAGSCPTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVD 215
           GAT+GELYY I +K++TLAFPAG CPTVGVGGH SGGGYG L+RK+GLAADHVIDA +VD
Sbjct: 156 GATIGELYYEIGKKNRTLAFPAGVCPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVD 215

Query: 216 ANGKVHNKESMGEDLFWAIRGGGGGCFGIVVAWKVKLVEVPARVTICEANRTLEEDRILK 275
           A G++  +  MGED FWAIRGGGG  F +V++WK+ L+ VP+ VT+    +   E   LK
Sbjct: 216 ARGRILERREMGEDFFWAIRGGGGSSFCVVLSWKIGLINVPSTVTVFNVTK-FSEQSALK 275

Query: 276 LIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTF 335
           +IH WQ+VA K+  +L + + L        +  K    ASF  ++LG  + +L ++   F
Sbjct: 276 IIHRWQFVADKVSDDLFIRVML--------QRYKNMVRASFPGLYLGSVKNLLKMVNKEF 335

Query: 336 PQLSLTKEDCLEMSWIQSVLLMGWFQK--GDPLEVLLNRTRLYSEISKIKSDYVKEHIPL 395
           P+L L ++DC EMSWI+SV+   WF +   +P+ VL  RTR  S   K KSD+V+E +P 
Sbjct: 336 PELGLEEDDCTEMSWIESVI---WFAELGEEPINVLTKRTRA-SLAFKAKSDFVQEPMPK 395

Query: 396 VALKGMWERLKSKDVELSQIMFVPYGGKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSV 455
            A+  +W RL+  + E +Q++F P+GGKMSE+ D ETPF HR    Y + Y+  W+    
Sbjct: 396 TAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIADYETPFPHRKGNIYEIQYLNYWRG--- 455

Query: 456 EAEERHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFG 515
           + +E+++ W+  +YD M+ FV+KSPR AY+N RDLD+G      R+ Y+  + WG+KYF 
Sbjct: 456 DVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFK 515

Query: 516 KNFDKLVYVKTKVDPSDFFRHQQSIP 540
            NF++LV VKT VDPSDFF  +QSIP
Sbjct: 516 NNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Cmc12g0327391 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 489.2 bits (1258), Expect = 4.4e-138
Identity = 248/542 (45.76%), Postives = 352/542 (64.94%), Query Frame = 0

Query: 10  PFGLIAFIIVVVL-----STATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPSN--S 69
           P   I+ IIV VL         TS      D F+ C+    +  S P+   ++TP+   S
Sbjct: 6   PLPTISCIIVSVLYFSFYCITPTSSSASIQDQFINCV-KRNTHVSFPLEKTLFTPAKNVS 65

Query: 70  SYSSILNFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEG 129
            ++ +L  + QNL+FL K++ KP  I  P H S +QA++ICSK  G   R RSGGHDFE 
Sbjct: 66  LFNQVLESTAQNLQFLAKSMPKPGFIFRPIHQSQVQASIICSKKLGIHFRVRSGGHDFEA 125

Query: 130 LSYISGV--PFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSC 189
           LSY+S +  PF+++DL  L  I++D+E+ +AWV+ GATLGELYYRIAEKS+   FPAG C
Sbjct: 126 LSYVSRIEKPFILLDLSKLKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLC 185

Query: 190 PTVGVGGHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGG 249
            +VG+GG+++GGGYG L+RKYGLA D+V+D  +VDANGK+ ++ +MGEDLFWAIRGGGG 
Sbjct: 186 TSVGIGGYMTGGGYGTLMRKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGA 245

Query: 250 CFGIVVAWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGG 309
            FGIV+AWK+KLV VP  VT+    +TLE+D  LK I +WQ ++ K+   + + + L   
Sbjct: 246 SFGIVLAWKIKLVPVPKTVTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAA 305

Query: 310 KVTEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWF 369
                 +G      ++   FLG+   +L +++  FP+L LT++DC EMSWI++ L  G F
Sbjct: 306 ----GNDGNKTVTMTYLGQFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGF 365

Query: 370 QKGDPLEVLLN-RTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYG 429
             G P+E+LL  ++ L  +  K  SD+VKE IP++ LKG+++RL   +     + + PYG
Sbjct: 366 PTGSPIEILLQLKSPLGKDYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTF--LNWTPYG 425

Query: 430 GKMSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPR 489
           G MS++ +S  PF HR+   + + Y A W     +   R +NWI+EIY+YM P+VS +PR
Sbjct: 426 GMMSKIPESAIPFPHRNGTLFKILYYANWLEND-KTSSRKINWIKEIYNYMAPYVSSNPR 485

Query: 490 AAYVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIP 542
            AYVNYRDLD G N    + ++  A++WG KYF  NFD+LV +KTKVDP +FFRH+QSIP
Sbjct: 486 QAYVNYRDLDFGQNKNNAKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIP 539

BLAST of Cmc12g0327391 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 488.0 bits (1255), Expect = 9.8e-138
Identity = 243/532 (45.68%), Postives = 358/532 (67.29%), Query Frame = 0

Query: 16  FIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPSNSS--YSSILNFSIQ 75
           F++ ++L +   S  T + D FV+CL D  S  S PI    ++P  ++  +   L  + Q
Sbjct: 13  FLVTLLLISVPLSSSTLQQD-FVKCLVD-NSDVSFPITASFFSPDQNATLFKEELESTAQ 72

Query: 76  NLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYIS--GVPF 135
           NLR+L  +  KP+ I  P + +H+QAAV+C+K     +R RSGGHD+EGLS+++    PF
Sbjct: 73  NLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETPF 132

Query: 136 VMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSCPTVGVGGHLSG 195
           V+VDL  L  + +D+++ +AW  +GAT+GE+YYRI EKSQT  FPAG C ++G+GGHL G
Sbjct: 133 VIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVG 192

Query: 196 GGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGCFGIVVAWKVK 255
           G YG ++RK+GL AD+V+DA +VDANG++ ++ +MGED+FWAIRGGGGG FG+++AWK+K
Sbjct: 193 GAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIK 252

Query: 256 LVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISLTGGKVTEDEEGKIN 315
           LV VPA VT+    +TLE+D   K++++W+ +A KLD +L + + ++    T  + G   
Sbjct: 253 LVPVPATVTVFTVTKTLEQDG-TKVLYKWEQIADKLDDDLFIRVIISPASKT-TKPGNRT 312

Query: 316 PIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWFQKGDPLEVLLN 375
              S+   FLG +  +L +++ +FP+L LTK+DC EMSWI+SV+ +  F      E LL 
Sbjct: 313 ISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLA 372

Query: 376 RTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGGKMSEVDDSETP 435
              L+    K KSD+VKE IP+  L+G+WER   +D  L+  ++ PYGG MS + +SE P
Sbjct: 373 GKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISESEIP 432

Query: 436 FSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIG 495
           F HR+   + + +++ W++G V +EERH+ WIRE+Y YM  +VSK+PR AYVNYRDLD+G
Sbjct: 433 FPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLG 492

Query: 496 TNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPTLSG 544
           TN   G T    AR WG KY+  NF++LV +K + DP +FFRH+QS+PT  G
Sbjct: 493 TNE--GETD---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPTKIG 532

BLAST of Cmc12g0327391 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 487.3 bits (1253), Expect = 1.7e-137
Identity = 251/529 (47.45%), Postives = 359/529 (67.86%), Query Frame = 0

Query: 17  IIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTP--SNSSYSSILNFSIQN 76
           ++++ LS    SF T + D FV CL D  S  S P+ +  +T   + SS+   L  S QN
Sbjct: 16  LLLLSLSYIPLSFSTIQQD-FVMCLVD-NSDASFPMDSSFFTHDLNASSFKLALETSAQN 75

Query: 77  LRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISGV--PFV 136
           LR+L  +  KP  I  P + +H+QAAV+C+K     +R RSGGHD+EGLSY+S +   FV
Sbjct: 76  LRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETAFV 135

Query: 137 MVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSCPTVGVGGHLSGG 196
           +VDL  L  IS+D+E+ +AWV +GA++GE+YYRI EKS+   FPAG C ++G+GGH+ GG
Sbjct: 136 IVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGG 195

Query: 197 GYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGCFGIVVAWKVKL 256
            YG ++RK+GL AD+V+DA +VDA+GK+ N+ +MGED+FWAIRGGGGG FG+++AWK+KL
Sbjct: 196 AYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKL 255

Query: 257 VEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANLLLGISL-TGGKVTEDEEGKIN 316
           V VP  VT+    RTLE+D   KL+++WQ VA KLD +L + + +    K  + +E  I+
Sbjct: 256 VPVPEIVTVFTVTRTLEQDG-TKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTIS 315

Query: 317 PIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWFQKGDPLEVLLN 376
              S+   FLG A  +L +++ +FPQL LTK+DCLE SWI+SV+ +  F    P E LL+
Sbjct: 316 --TSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD 375

Query: 377 RTRLYSEISKIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGGKMSEVDDSETP 436
              L+    K KSDYV+E IP+  L+G+WE+L  +D  L+  ++ PYGG M+++ ++ETP
Sbjct: 376 GKSLFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIPETETP 435

Query: 437 FSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRAAYVNYRDLDIG 496
           F HRS   + + ++  W++G   +E +H+ W+RE+Y YM  +VSKSPR+AYVNYRDLD+G
Sbjct: 436 FPHRSGTLFKIQWLTLWQDGKT-SEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLG 495

Query: 497 TNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPT 541
            N K        AR WG +YF  NF++LV +K K DP +FFRH+QSIPT
Sbjct: 496 MNGK-----GSDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPT 531

BLAST of Cmc12g0327391 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 486.1 bits (1250), Expect = 3.7e-137
Identity = 241/540 (44.63%), Postives = 359/540 (66.48%), Query Frame = 0

Query: 11  FGLIAFIIVVVLSTATTSFCTQECDSFVQCLFDYGSPNSNPIANVIYTPSN--SSYSSIL 70
           F ++     +   T T+S  + + D F+ C+    +    P+    + P+   S +S +L
Sbjct: 10  FSILVLYFSLYTITPTSSLASLQ-DQFINCV-QRNTHVYFPLEKTFFAPTKNVSMFSQVL 69

Query: 71  NFSIQNLRFLNKNISKPLAIITPSHVSHIQAAVICSKSHGFQIRTRSGGHDFEGLSYISG 130
             + QNLRFL K++ KP  I +P H SH+QA++ICSK     +R RSGGHD+EGLSY+S 
Sbjct: 70  ESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQ 129

Query: 131 V--PFVMVDLINLNSISIDVENQNAWVESGATLGELYYRIAEKSQTLAFPAGSCPTVGVG 190
           +  PF+++DL  +  ++I++++ +AWV+SGAT+GELYYRIAEKS+   FPAG C ++G+G
Sbjct: 130 IDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIG 189

Query: 191 GHLSGGGYGWLIRKYGLAADHVIDAYLVDANGKVHNKESMGEDLFWAIRGGGGGCFGIVV 250
           GH++GG YG ++RKYGL AD+V+DA +VDANGK+ ++ +MGED FWAIRGG GG FGI++
Sbjct: 190 GHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIIL 249

Query: 251 AWKVKLVEVPARVTICEANRTLEEDRILKLIHEWQYVAPKLDANL----LLGISLTGGKV 310
           AWK+KLV VP  VT+    +TL++D   K+I +WQ VA KL   L    L  ++ TGG  
Sbjct: 250 AWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGTGGNK 309

Query: 311 TEDEEGKINPIASFSLMFLGKAEEVLTILKPTFPQLSLTKEDCLEMSWIQSVLLMGWFQK 370
           T           S++ +FLG    ++ ++K +FP+L LT +DC+EMSW++S+  +  F  
Sbjct: 310 T--------VTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPT 369

Query: 371 GDPLEVLLNRTRLYSEIS-KIKSDYVKEHIPLVALKGMWERLKSKDVELSQIMFVPYGGK 430
             P  VLL     + ++S K KSD+VK  IP   L+G++++L  +D+ L  +++ PYGG 
Sbjct: 370 HTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGM 429

Query: 431 MSEVDDSETPFSHRSDYSYLVGYIAKWKNGSVEAEERHLNWIREIYDYMTPFVSKSPRAA 490
           M+++ +S+ PF HR    + V Y+  W + S +   RH+NWIR++Y YMTP+VS +PR A
Sbjct: 430 MAKIPESQIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPREA 489

Query: 491 YVNYRDLDIGTNNKYGRTSYKRARVWGLKYFGKNFDKLVYVKTKVDPSDFFRHQQSIPTL 542
           YVNYRDLD+G N K  +T  K+A+VWG  YF  NF++L+ +K KVDP +FFRH+QSIP +
Sbjct: 490 YVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536