Cmc09g0256491 (gene) Melon (Charmono) v1.1

Overview
NameCmc09g0256491
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
LocationCMiso1.1chr09: 21339326 .. 21339910 (-)
RNA-Seq ExpressionCmc09g0256491
SyntenyCmc09g0256491
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAATCTCAAGATCTCACTACCACTTCTCATTACCCTCATAGCTCTCCACAATGCCGCAACCACAGGCTCGCCCTCCACAAGCTTCATCGAGTCATCCTGCAAAGTGACCCGTTACCCAGCGCTGTGCGTTCAGTCCCTCTCAACCTACGCCAACACCATCCGCCAGAGCGGTCGTCAGCTGGCCCGAACGGCCCTATCCGTGAGCCTCAGCAAGGCCCGTCTCGCCGCAGCTTTCGTGGCCAAACTGGGGAAAGGCGGCGGAATGAAGGGGCTGGAGTACCAAGCAGTGAAGGATTGCATAGAGAATATGGGGGATAGCGTGGACAGACTCAGCCAATCGGTGAAGGAGCTTGGGGACTTGAGGCGAACAGCTGGTAGAGACTTTTTATGGCATATGAATAATGTTCAGACTTGGGTCAGTGCTGCCCTCACTGATGAAACCACTTGCCTTGATGGGTTCGCTGGGCGCCGCTTAGATGGTCAAACCAAGGCGGGGATTCGCCGGAGAATTACACTTGTTGCTCAAATTACTAGTAATGCTCTGGCTCTTGTTAATCGCTTTGCTGACGAAAACCACTGA

mRNA sequence

ATGGCAAATCTCAAGATCTCACTACCACTTCTCATTACCCTCATAGCTCTCCACAATGCCGCAACCACAGGCTCGCCCTCCACAAGCTTCATCGAGTCATCCTGCAAAGTGACCCGTTACCCAGCGCTGTGCGTTCAGTCCCTCTCAACCTACGCCAACACCATCCGCCAGAGCGGTCGTCAGCTGGCCCGAACGGCCCTATCCGTGAGCCTCAGCAAGGCCCGTCTCGCCGCAGCTTTCGTGGCCAAACTGGGGAAAGGCGGCGGAATGAAGGGGCTGGAGTACCAAGCAGTGAAGGATTGCATAGAGAATATGGGGGATAGCGTGGACAGACTCAGCCAATCGGTGAAGGAGCTTGGGGACTTGAGGCGAACAGCTGGTAGAGACTTTTTATGGCATATGAATAATGTTCAGACTTGGGTCAGTGCTGCCCTCACTGATGAAACCACTTGCCTTGATGGGTTCGCTGGGCGCCGCTTAGATGGTCAAACCAAGGCGGGGATTCGCCGGAGAATTACACTTGTTGCTCAAATTACTAGTAATGCTCTGGCTCTTGTTAATCGCTTTGCTGACGAAAACCACTGA

Coding sequence (CDS)

ATGGCAAATCTCAAGATCTCACTACCACTTCTCATTACCCTCATAGCTCTCCACAATGCCGCAACCACAGGCTCGCCCTCCACAAGCTTCATCGAGTCATCCTGCAAAGTGACCCGTTACCCAGCGCTGTGCGTTCAGTCCCTCTCAACCTACGCCAACACCATCCGCCAGAGCGGTCGTCAGCTGGCCCGAACGGCCCTATCCGTGAGCCTCAGCAAGGCCCGTCTCGCCGCAGCTTTCGTGGCCAAACTGGGGAAAGGCGGCGGAATGAAGGGGCTGGAGTACCAAGCAGTGAAGGATTGCATAGAGAATATGGGGGATAGCGTGGACAGACTCAGCCAATCGGTGAAGGAGCTTGGGGACTTGAGGCGAACAGCTGGTAGAGACTTTTTATGGCATATGAATAATGTTCAGACTTGGGTCAGTGCTGCCCTCACTGATGAAACCACTTGCCTTGATGGGTTCGCTGGGCGCCGCTTAGATGGTCAAACCAAGGCGGGGATTCGCCGGAGAATTACACTTGTTGCTCAAATTACTAGTAATGCTCTGGCTCTTGTTAATCGCTTTGCTGACGAAAACCACTGA

Protein sequence

MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGRQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITSNALALVNRFADENH
Homology
BLAST of Cmc09g0256491 vs. NCBI nr
Match: KAA0039678.1 (21 kDa protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 372.9 bits (956), Expect = 1.7e-99
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0

Query: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60
           MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR
Sbjct: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60

Query: 61  QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120
           QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120

Query: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180
           DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS
Sbjct: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180

Query: 181 NALALVNRFADENH 195
           NALALVNRFADENH
Sbjct: 181 NALALVNRFADENH 194

BLAST of Cmc09g0256491 vs. NCBI nr
Match: XP_008437134.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 370.2 bits (949), Expect = 1.1e-98
Identity = 193/194 (99.48%), Postives = 193/194 (99.48%), Query Frame = 0

Query: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60
           MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR
Sbjct: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60

Query: 61  QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120
           QLARTALSVSLSKA LAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKALLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120

Query: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180
           DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS
Sbjct: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180

Query: 181 NALALVNRFADENH 195
           NALALVNRFADENH
Sbjct: 181 NALALVNRFADENH 194

BLAST of Cmc09g0256491 vs. NCBI nr
Match: XP_004147584.1 (21 kDa protein [Cucumis sativus] >KGN50211.1 hypothetical protein Csa_000459 [Cucumis sativus])

HSP 1 Score: 357.1 bits (915), Expect = 9.8e-95
Identity = 185/194 (95.36%), Postives = 190/194 (97.94%), Query Frame = 0

Query: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60
           MANLKISLPLLITL+ALHNAATTGS +TSFIESSCKVTRYPALCVQSLSTYAN IRQSGR
Sbjct: 1   MANLKISLPLLITLVALHNAATTGSAATSFIESSCKVTRYPALCVQSLSTYANVIRQSGR 60

Query: 61  QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120
           QLARTALSVSLSKARLA+AFVAKLGKGGGMKGLEYQAVKDCIENMGD+VDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKARLASAFVAKLGKGGGMKGLEYQAVKDCIENMGDTVDRLSQSVKELG 120

Query: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180
           DLR+TAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQ KA IRRRITLVAQITS
Sbjct: 121 DLRQTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAEIRRRITLVAQITS 180

Query: 181 NALALVNRFADENH 195
           NALALVNRFADENH
Sbjct: 181 NALALVNRFADENH 194

BLAST of Cmc09g0256491 vs. NCBI nr
Match: XP_038875547.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 338.2 bits (866), Expect = 4.7e-89
Identity = 178/195 (91.28%), Postives = 184/195 (94.36%), Query Frame = 0

Query: 1   MANLKISLPLLITLIALHNAATT-GSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSG 60
           MANLKISLP+LI L+ALHNAAT   S +TSFIESSCKVTRYPALCVQSLS+YA TIRQSG
Sbjct: 13  MANLKISLPILIILVALHNAATAESSAATSFIESSCKVTRYPALCVQSLSSYATTIRQSG 72

Query: 61  RQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 120
           RQLAR ALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRL QSVKEL
Sbjct: 73  RQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLGQSVKEL 132

Query: 121 GDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQIT 180
           GDLRRTAG DFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQ KA IR+RITLVAQIT
Sbjct: 133 GDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAAIRQRITLVAQIT 192

Query: 181 SNALALVNRFADENH 195
           SNALALVNRFAD+NH
Sbjct: 193 SNALALVNRFADKNH 207

BLAST of Cmc09g0256491 vs. NCBI nr
Match: KAG6606163.1 (Pectinesterase inhibitor 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 296.6 bits (758), Expect = 1.6e-76
Identity = 155/195 (79.49%), Postives = 173/195 (88.72%), Query Frame = 0

Query: 1   MANLKISLPLLITLIALHNAATTGS-PSTSFIESSCKVTRYPALCVQSLSTYANTIRQSG 60
           M NLKISLPLL+ L+ LHNAA T S  +TSFIESSC+VTRYPALCV SLS YA +IRQSG
Sbjct: 1   MPNLKISLPLLV-LVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSG 60

Query: 61  RQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 120
           RQL +TALSVSL+K+RLAAAFV+ L KGG ++GLEYQA+KDCIENMGD+VDRL QSVKEL
Sbjct: 61  RQLTQTALSVSLNKSRLAAAFVSNLAKGGAVRGLEYQALKDCIENMGDTVDRLRQSVKEL 120

Query: 121 GDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQIT 180
            DLRRTAG D LWH++NVQTWVSAALTDETTCLDGFA RRLDGQ KA IRR+ITLVAQ+T
Sbjct: 121 RDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVT 180

Query: 181 SNALALVNRFADENH 195
           SNALALVN FAD+NH
Sbjct: 181 SNALALVNHFADKNH 194

BLAST of Cmc09g0256491 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 5.7e-45
Identity = 89/181 (49.17%), Postives = 131/181 (72.38%), Query Frame = 0

Query: 10  LLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGRQLARTALSV 69
           L+ + + +  AA + +P+  FI++SC +T YPA+C QSLS YA TI+ + ++LA TAL V
Sbjct: 8   LVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQV 67

Query: 70  SLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTAGRD 129
           SL++ + A  F+ +L K  G+K  +Y A+ DC+E + DS+DR+S+S  E+ +L    G D
Sbjct: 68  SLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGND 127

Query: 130 FLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITSNALALVNRF 189
           F + M+NV+TWVSAALTDETTC+DGFAG+ +DG+ K  +R ++  VA++TSNALALVN F
Sbjct: 128 FTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNNF 187

Query: 190 A 191
           A
Sbjct: 188 A 188

BLAST of Cmc09g0256491 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.8e-44
Identity = 103/186 (55.38%), Postives = 131/186 (70.43%), Query Frame = 0

Query: 10  LLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGRQ-LARTALS 69
           L IT++   +A    S S  FI SSC+ T+YP+LCV +LS YA  IR +  Q LA+TAL 
Sbjct: 13  LSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTALI 72

Query: 70  VSLSKARLAAAFVAKLGK-GGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDL-RRTA 129
           +SL++A+    FVAKL K     K  EY A+KDCIE +G+SVDRL+QSVKEL       A
Sbjct: 73  ISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHAVA 132

Query: 130 GRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITSNALALV 189
             DF+W M+NVQTWVSAALTDETTCLDGF+ R + G+ K  IR ++  VAQ+TSNALALV
Sbjct: 133 SEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALALV 192

Query: 190 NRFADE 193
           N+FA++
Sbjct: 193 NQFAEK 198

BLAST of Cmc09g0256491 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 7.2e-40
Identity = 90/186 (48.39%), Postives = 124/186 (66.67%), Query Frame = 0

Query: 6   ISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGRQLART 65
           +S  +L    +   AA T   + +FI++SCK T YP +CV SL+ YAN+I+ S R+LA T
Sbjct: 14  LSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAET 73

Query: 66  ALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRT 125
           AL+V++++A+    FV +LG+   +K  E QAVKDCIE + D+VDRL+ S+ E+      
Sbjct: 74  ALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSA 133

Query: 126 AGRDFLW-HMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITSNALA 185
            GRD  W HM+N QTW SAALT+  TC DGFAGR +DG+ K  +R RI  + + TSNALA
Sbjct: 134 KGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALA 193

Query: 186 LVNRFA 191
           L+N FA
Sbjct: 194 LINAFA 199

BLAST of Cmc09g0256491 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.1e-39
Identity = 89/198 (44.95%), Postives = 132/198 (66.67%), Query Frame = 0

Query: 3   NLKISLPLLI------TLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIR 62
           N ++SL L +       ++   N     S  T FI++SC+ T YP  C QSLS+YA+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 63  QSGRQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSV 122
           +  R+LA TAL+VS+++A+ A  +V+++    G+   +++AV DC+E MGD+VDRLS S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 123 KELGDLRR-TAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLV 182
           KEL  L    +G DF + ++NV+TW SAALTDET C+DGF G+ + G+ K+ IR  I  V
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 183 AQITSNALALVNRFADEN 194
           A+ TSNALAL+N FA ++
Sbjct: 184 AEETSNALALINDFASKH 201

BLAST of Cmc09g0256491 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.0e-30
Identity = 75/184 (40.76%), Postives = 115/184 (62.50%), Query Frame = 0

Query: 7   SLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGRQLARTA 66
           S PL     +  ++  +   +  +I++SC +T Y  +C  SLS YA+TIR + ++LA  A
Sbjct: 124 SSPLSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIA 183

Query: 67  LSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRTA 126
           L+++LS A+ A+ FV  +  GGG+  LE  AV DC+E +GDSV  L  S++EL  +    
Sbjct: 184 LNLTLSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKD 243

Query: 127 GRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITSNALALV 186
              F   M++V+TWVSAALT++ TC+DGF+   +    K  +RR +  VA++TSNALAL+
Sbjct: 244 SAKFEMVMSDVETWVSAALTNDDTCMDGFS--LVKTAVKDLVRRHVVEVARLTSNALALI 303

Query: 187 NRFA 191
           N +A
Sbjct: 304 NMYA 305

BLAST of Cmc09g0256491 vs. ExPASy TrEMBL
Match: A0A5A7T9V3 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00090 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 8.4e-100
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0

Query: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60
           MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR
Sbjct: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60

Query: 61  QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120
           QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120

Query: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180
           DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS
Sbjct: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180

Query: 181 NALALVNRFADENH 195
           NALALVNRFADENH
Sbjct: 181 NALALVNRFADENH 194

BLAST of Cmc09g0256491 vs. ExPASy TrEMBL
Match: A0A1S3ATE5 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103482647 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.4e-99
Identity = 193/194 (99.48%), Postives = 193/194 (99.48%), Query Frame = 0

Query: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60
           MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR
Sbjct: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60

Query: 61  QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120
           QLARTALSVSLSKA LAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKALLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120

Query: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180
           DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS
Sbjct: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180

Query: 181 NALALVNRFADENH 195
           NALALVNRFADENH
Sbjct: 181 NALALVNRFADENH 194

BLAST of Cmc09g0256491 vs. ExPASy TrEMBL
Match: A0A0A0KR60 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G160140 PE=4 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 4.8e-95
Identity = 185/194 (95.36%), Postives = 190/194 (97.94%), Query Frame = 0

Query: 1   MANLKISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGR 60
           MANLKISLPLLITL+ALHNAATTGS +TSFIESSCKVTRYPALCVQSLSTYAN IRQSGR
Sbjct: 1   MANLKISLPLLITLVALHNAATTGSAATSFIESSCKVTRYPALCVQSLSTYANVIRQSGR 60

Query: 61  QLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELG 120
           QLARTALSVSLSKARLA+AFVAKLGKGGGMKGLEYQAVKDCIENMGD+VDRLSQSVKELG
Sbjct: 61  QLARTALSVSLSKARLASAFVAKLGKGGGMKGLEYQAVKDCIENMGDTVDRLSQSVKELG 120

Query: 121 DLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 180
           DLR+TAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQ KA IRRRITLVAQITS
Sbjct: 121 DLRQTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAEIRRRITLVAQITS 180

Query: 181 NALALVNRFADENH 195
           NALALVNRFADENH
Sbjct: 181 NALALVNRFADENH 194

BLAST of Cmc09g0256491 vs. ExPASy TrEMBL
Match: A0A6J1H2L8 (pectinesterase inhibitor 9-like OS=Cucurbita moschata OX=3662 GN=LOC111459863 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 4.2e-75
Identity = 153/195 (78.46%), Postives = 171/195 (87.69%), Query Frame = 0

Query: 1   MANLKISLPLLITLIALHNAATTGS-PSTSFIESSCKVTRYPALCVQSLSTYANTIRQSG 60
           M NLKISLPLL+ L+ LHNAA T S  +TSFIESSC+VTRYPALCV SLS YA +IRQS 
Sbjct: 1   MPNLKISLPLLV-LVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSS 60

Query: 61  RQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 120
           RQL +TALSVSL+K+RLAAAFV+ L KGG ++GLEYQA+KDCIENMGD+VDRL QSVKEL
Sbjct: 61  RQLTQTALSVSLNKSRLAAAFVSNLAKGGAVRGLEYQALKDCIENMGDTVDRLRQSVKEL 120

Query: 121 GDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQIT 180
            DLRRTAG D LWH++NVQTWVSAALTDETTCLDGFA RRLDGQ KA IRR+ITLVAQ+T
Sbjct: 121 RDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAVIRRKITLVAQVT 180

Query: 181 SNALALVNRFADENH 195
           SNALALVN FAD+ H
Sbjct: 181 SNALALVNHFADKKH 194

BLAST of Cmc09g0256491 vs. ExPASy TrEMBL
Match: A0A6J1K807 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111491486 PE=4 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 1.2e-74
Identity = 152/195 (77.95%), Postives = 170/195 (87.18%), Query Frame = 0

Query: 1   MANLKISLPLLITLIALHNAATTGS-PSTSFIESSCKVTRYPALCVQSLSTYANTIRQSG 60
           M NLKISLPLL+ L+ LHNAA T S  +TSFIESSC+VTRYPALCV SLS YA +IRQSG
Sbjct: 22  MPNLKISLPLLV-LVVLHNAAPTHSATATSFIESSCRVTRYPALCVHSLSAYATSIRQSG 81

Query: 61  RQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKEL 120
           RQL +TALSVSL+K+R AAAFV+ L KG  ++GLEYQA+KDCIENMGD+VDRL QSVKEL
Sbjct: 82  RQLTQTALSVSLNKSRYAAAFVSNLAKGRAVRGLEYQALKDCIENMGDTVDRLRQSVKEL 141

Query: 121 GDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQIT 180
            DLRRTAG D LWH++NVQTWVSAALTDETTCLDGFA RRLDGQ KA IRR+ITLVAQ+T
Sbjct: 142 RDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAAIRRKITLVAQVT 201

Query: 181 SNALALVNRFADENH 195
           SNALALVN FAD+ H
Sbjct: 202 SNALALVNHFADKKH 215

BLAST of Cmc09g0256491 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 183.3 bits (464), Expect = 1.8e-46
Identity = 102/191 (53.40%), Postives = 140/191 (73.30%), Query Frame = 0

Query: 4   LKISLPLLI-TLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGRQL 63
           L + LPLL  + IA  +++   S S +FI SSC+VTRY  LCV+ L+ +A+ IR++  QL
Sbjct: 12  LLLFLPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRNENQL 71

Query: 64  ARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDL 123
           A+TAL+V+L + +    +V KL K   +K  EY AVKDC+EN+GD ++ L+QS++EL  +
Sbjct: 72  AQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRELKQV 131

Query: 124 RRTAGRD---FLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQIT 183
            R +GRD   FLW ++NV+TWVSAALTDETTCLDGF G+ +DG  K+ IRRR+  VA++T
Sbjct: 132 GR-SGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVT 191

Query: 184 SNALALVNRFA 191
           SNALALVNRFA
Sbjct: 192 SNALALVNRFA 201

BLAST of Cmc09g0256491 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 179.9 bits (455), Expect = 2.0e-45
Identity = 103/186 (55.38%), Postives = 131/186 (70.43%), Query Frame = 0

Query: 10  LLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGRQ-LARTALS 69
           L IT++   +A    S S  FI SSC+ T+YP+LCV +LS YA  IR +  Q LA+TAL 
Sbjct: 13  LSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQDLAQTALI 72

Query: 70  VSLSKARLAAAFVAKLGK-GGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDL-RRTA 129
           +SL++A+    FVAKL K     K  EY A+KDCIE +G+SVDRL+QSVKEL       A
Sbjct: 73  ISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELARAGHAVA 132

Query: 130 GRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITSNALALV 189
             DF+W M+NVQTWVSAALTDETTCLDGF+ R + G+ K  IR ++  VAQ+TSNALALV
Sbjct: 133 SEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVTSNALALV 192

Query: 190 NRFADE 193
           N+FA++
Sbjct: 193 NQFAEK 198

BLAST of Cmc09g0256491 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 2.4e-43
Identity = 97/190 (51.05%), Postives = 131/190 (68.95%), Query Frame = 0

Query: 4   LKISLPLLITLIALHNAATTGS--PSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGRQ 63
           L IS  L + L A   A+ TG+   + +FI+SSCK T YPALCV SLS YAN I+ S ++
Sbjct: 10  LSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPKR 69

Query: 64  LARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGD 123
           LA TA++V+LS+A+    FV++L +  G+K  E +A+KDC+E M D+VDRL++SV+EL  
Sbjct: 70  LAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKL 129

Query: 124 LRRTAGRD-FLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITS 183
                 +D F +HM+N QTW SAALTDE TC DGF+GR +DG+ K  +R RI  V   TS
Sbjct: 130 CGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETS 189

Query: 184 NALALVNRFA 191
           NAL+L+N FA
Sbjct: 190 NALSLINAFA 199

BLAST of Cmc09g0256491 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 5.1e-41
Identity = 90/186 (48.39%), Postives = 124/186 (66.67%), Query Frame = 0

Query: 6   ISLPLLITLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIRQSGRQLART 65
           +S  +L    +   AA T   + +FI++SCK T YP +CV SL+ YAN+I+ S R+LA T
Sbjct: 14  LSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAET 73

Query: 66  ALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSVKELGDLRRT 125
           AL+V++++A+    FV +LG+   +K  E QAVKDCIE + D+VDRL+ S+ E+      
Sbjct: 74  ALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSA 133

Query: 126 AGRDFLW-HMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLVAQITSNALA 185
            GRD  W HM+N QTW SAALT+  TC DGFAGR +DG+ K  +R RI  + + TSNALA
Sbjct: 134 KGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALA 193

Query: 186 LVNRFA 191
           L+N FA
Sbjct: 194 LINAFA 199

BLAST of Cmc09g0256491 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 1.5e-40
Identity = 89/198 (44.95%), Postives = 132/198 (66.67%), Query Frame = 0

Query: 3   NLKISLPLLI------TLIALHNAATTGSPSTSFIESSCKVTRYPALCVQSLSTYANTIR 62
           N ++SL L +       ++   N     S  T FI++SC+ T YP  C QSLS+YA+ I+
Sbjct: 4   NFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 63

Query: 63  QSGRQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDRLSQSV 122
           +  R+LA TAL+VS+++A+ A  +V+++    G+   +++AV DC+E MGD+VDRLS S+
Sbjct: 64  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 123

Query: 123 KELGDLRR-TAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRRITLV 182
           KEL  L    +G DF + ++NV+TW SAALTDET C+DGF G+ + G+ K+ IR  I  V
Sbjct: 124 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 183

Query: 183 AQITSNALALVNRFADEN 194
           A+ TSNALAL+N FA ++
Sbjct: 184 AEETSNALALINDFASKH 201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0039678.11.7e-99100.0021 kDa protein-like [Cucumis melo var. makuwa][more]
XP_008437134.11.1e-9899.48PREDICTED: 21 kDa protein-like [Cucumis melo][more]
XP_004147584.19.8e-9595.3621 kDa protein [Cucumis sativus] >KGN50211.1 hypothetical protein Csa_000459 [Cu... [more]
XP_038875547.14.7e-8991.2821 kDa protein-like [Benincasa hispida][more]
KAG6606163.11.6e-7679.49Pectinesterase inhibitor 9, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
P174075.7e-4549.1721 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1[more]
Q9SI722.8e-4455.38Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1[more]
Q9STY57.2e-4048.39Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1[more]
Q9SB372.1e-3944.95Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1[more]
Q9SI741.0e-3040.76Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7T9V38.4e-100100.0021 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G... [more]
A0A1S3ATE55.4e-9999.4821 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103482647 PE=4 SV=1[more]
A0A0A0KR604.8e-9595.36PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G160140 PE=4 S... [more]
A0A6J1H2L84.2e-7578.46pectinesterase inhibitor 9-like OS=Cucurbita moschata OX=3662 GN=LOC111459863 PE... [more]
A0A6J1K8071.2e-7477.9521 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111491486 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G12390.11.8e-4653.40pectin methylesterase inhibitor 1 [more]
AT1G62770.12.0e-4555.38Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G62350.12.4e-4351.05Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT3G47380.15.1e-4148.39Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT4G25260.11.5e-4044.95Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 26..185
e-value: 7.7E-54
score: 194.8
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 1..187
e-value: 2.2E-37
score: 126.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 29..185
e-value: 3.9E-41
score: 140.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 26..189
e-value: 9.5E-41
score: 141.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 29..189
NoneNo IPR availablePANTHERPTHR31080:SF207PECTINESTERASE INHIBITOR 9coord: 14..192
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 14..192
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 33..190
e-value: 4.55532E-45
score: 144.503

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc09g0256491.1Cmc09g0256491.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0004857 enzyme inhibitor activity