Sgr021594 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr021594
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionnephrocystin-3 isoform X1
Locationtig00153764: 334520 .. 338080 (+)
RNA-Seq ExpressionSgr021594
SyntenySgr021594
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGTCATTGCAGTTGCAAAAGGGCATCACTTGCTCCTCAGTTTACCTCCAAAAACAAAAATGCAATATCAAGCTTTATGCCGTACCTGTTAGAGCCTTTTCTTGTCACTTTGCCTCAACACCATCTGCTTCAAGTAGAGCTGATATGGGGGGTCAGAGGAAACATATTTCTTCTGCCTTCACTGCTCCAAATGGATATCAAAGGTTGGGACCAAATTTGGTAATTTCATTATTGTCTTTGAGAATGCTGTCAAGAGTTGACTATGTCATCCAGATAAAAGATTGAATGAATGGTGTTTATTTTTTAGCAGGTCTGATCACATGCATTACCGTACTGATGAGAACAGCATGAGTGAATTTGAGGGTCAGTTGGAAGAATTATTCAATGAAGTCAGAATGATGATTATGAGCGGGAGGAAGAATGATGCTGTAGAGTTACTTCAAGCAAATTATGAAGCTGTAAAAGAACAGATGGAATCAGGTGCTAATGGCATTGAACAAGCTGCTGTTCTTGACATCGTGGCTTTGGGGTATATAACTGTTGGAGACTTGAAGTTTGTTGCTTCTATATTGGATATAGTAACTTCTTTTTCCCTTATCTATCTGTCCAATAATTGATTTGAGAAACTCAATGCTTGTCAGTTTTGTGCAACAATGTTTATACTTTCCATACTTGTTAGGATTGTTTTATGTTTAGGTTATCATCTACCCATAATTGCATTCAGGGGATTTTCAAGTGCTAAAATTTTATCAATAACCTATAACCTGGCATTTGTTAATTGGAGTCGTTTAGCCCATGTAGTGTTGACTTTATGATGGCCGGTATATAGATGGATGACATGGACATATATAGGACTCAGAAATTCCTTTTTTTTTTTTTTTTCTCTTGATGACATTTTGATTCTGTTTATAAGTTATATGAGCATGTTGTTTGTTGCCTTTATCTTGCTCAAAGACAAGTAGACATGGTCATCTAGCAATGTAGTCACATCTTTTAGGTGATTTTGCAGTAATAAAGAGGTGACTTTTAACGTTTATCTCATTCTTACGCCCCAAAAGTATCCCATACTTCTACAGCCAATATTTCAGTTGCTGTTTTCATAATCGTTTGTAGGCCAATCTTCTACTCTCTTCAATTTTCTCCTGAGTGACATTCTTCTCTTCTTGTTTCCTTCTTCTGATGGAGTTTCATGTATGCAGTTGAACAAGGTCGTTGACAGTCTGAAGGACAGCGAACCCTTTCTGGATTCAGTGCTTCTGCATATGGGGAGCATGTACTCAACTTTGAAGAAGTTTGAGAAATCAATATCTGTGTACAAGAGGGCTATTGATATCATAGAGAAGAAAAGTGGTACGGGAGCTAGTGGGGACAAGTATTTAATTGTCCATCTAATGCTTTTTAAATAATTTTTCTCAAGTTCTCCTGTTACAGTTTACTTTCTGATTGAATATGTCTATCACCACCTTATTTTTAAGGTTCTCCCATCCCAATGATCTCATGAAGAATAAAATATTTTTCTAATAAGACCAGAAAGAAGAAAATCTAACAAGCTATTTTCTTTATTTGAATTCCGCAGCTATAATTGGGTTTTGCACTTTGTTGACTTTATCTTGTTTTCATTTGTTCTTCTATTCCTCTTTAAATCTTCTTACAAAGACTCCTATGCAGGAAAAGATAGTAGCTTTCTTATCACTCCAATTCTAGGAATGGCAAAAGTGCTTGGTACGATTGGAAGAATCTCCAAAGCAGTACAGTTTTACCATCGTGCAATTTCAATTTTGGAATCCAGCAGAGGCTTTGAGAACGAGGATTTGGTTATACCTTTATTTAGTCTGGGCAATCTTTTGCTCAAAGAAGGAAAAGGCAAGGATGCTGAAGCTTGTTTTGCTAGGTGCTCTCTCTCTCTCTCTCTCCTCTTCCTCCTTCCTTTGGACTATTCACTTACTTTTATGGTTCTGTCATAGGATTGTGAACATATATAAAAAGTTATATGGAGAGAAAGATGGAAAAGTTGGAATGGCTATGTGTTCTCTGGCTAATGCAAAGTGTGCAAGAGGTAACTTCTGTGTAGACTTGTGTTATCTTTCAAACACCAGCAAGGTCGTTTATTTGCAGAATGTTCTTCCTCTTATCCATAGGGGAAGCAGAAGAAGCTGTCATCTTATATAGAAGAGCTTTGCAGATTATCAAGGATTCAAATTATATGGCTTTAGATGACAGCGTGCTGGAGAAGATGAGGATTGATTTGGCAGAACTACTGCATGTTGTAGGAAGGTAATGTCATACTGATATTGGCATTGTTTTTACTCAGTTCATGTTGAAACCTTGTCACAGAATACTGATAGGTCCAGTTCGATATTGCCAAACAGAGTGTTACAAGCTAAGGGAAGTTTCGAGAGCTCCCGCTTCTCCACTGCCCAACTAACCGATGACTCAAATGATAATCCCCTCATTGACACTCTTTACGTGTATATATCTATTACAAAATTTACCCGTGTACCCCATTAACTAAATAACATTCCCATGGGTCACACTACTCATCACAAACACACAATACCTCTCACTTCCCAGCCAAGTCATCACCTCTCGCTCTCTTTCTACGATATATATTTCTGATTGGGGTCTATCAAATACTTCTAAAATCTTCTGTGTAAATGAATTCCAGTTGTTTTTGCACAGATTTATGGCTCATTGTGAATAAAAACATCTTCAACACGTTGATGATATAAACCTTTGAAGAAAATATTATTCTTTCACACATCATGTATGGTTACATAGTTCATTTCTTTTACCACCAGTGTTCATGAATTCCTGGAATTTGATATTCTAAAAATGAATTACTGAAATTTGGTTTGTTGCAGAGGAAAAGAAGGCAGAGAACTGCTAGAAGAGTGCTTGTTGATCAATGAAAAGTCAAAAGGAAAAGAGCATCCTAGCTCAGTGAAGCACCTTGTAAACCTTGCAGCATCGTATTCACGGTCCAAGAATTACGCAGAGGCTGAGCGTTTGTTGCAAATTGGATTGGACATTATGATTAAGGCAGCGGGACCGGATGACCAATCAATTACAGTCCCAATGTTGCATCTTTCTGTCACTCTTTACAATCTAAAACGAGACGAAGATGCCGAGCAACTTGCGTTGGAAGTTTTGCGAATACGGGAAAATGCATTTGGAAAAGATTCTCTTCCTGTTGGTGAGAATTTTCTTCGTTTTCTACTTGCTGGAGAATTGTTTTTAGATCACTTGATTTGCCATCTTCACATGAGCTCATCTTTGTCTTGAATTTTGATTTGGTGGCTGCACGCAGGTGAGGCTCTAGACTGTTTGGTTTCCATTCAGAGCAGGCTGGGGAAGGATGAAAACGAGGTACTGAAGATGCTGAAGAGAATTCTAAGCATCCAGGAGAAAGAGTTCGGGTTCGAGGGTAAAGAGGTCATTGACACCCTCAAGAAAATTGTGTTCTACATGGACAAACTAGGAATGAAAGCTGAGAAATTTCCACTTCAAAAACGACTGTCCATGCTTCGAATGAAATTCAAGAACCACATGCAATACTAA

mRNA sequence

ATGCAGTCATTGCAGTTGCAAAAGGGCATCACTTGCTCCTCAGTTTACCTCCAAAAACAAAAATGCAATATCAAGCTTTATGCCGTACCTGTTAGAGCCTTTTCTTGTCACTTTGCCTCAACACCATCTGCTTCAAGTAGAGCTGATATGGGGGGTCAGAGGAAACATATTTCTTCTGCCTTCACTGCTCCAAATGGATATCAAAGGTCTGATCACATGCATTACCGTACTGATGAGAACAGCATGAGTGAATTTGAGGGTCAGTTGGAAGAATTATTCAATGAAGTCAGAATGATGATTATGAGCGGGAGGAAGAATGATGCTGTAGAGTTACTTCAAGCAAATTATGAAGCTGTAAAAGAACAGATGGAATCAGGTGCTAATGGCATTGAACAAGCTGCTGTTCTTGACATCGTGGCTTTGGGGTATATAACTGTTGGAGACTTGAAGTTTGTTGCTTCTATATTGGATATATTGAACAAGGTCGTTGACAGTCTGAAGGACAGCGAACCCTTTCTGGATTCAGTGCTTCTGCATATGGGGAGCATGTACTCAACTTTGAAGAAGTTTGAGAAATCAATATCTGTGTACAAGAGGGCTATTGATATCATAGAGAAGAAAAGTGGAAAAGATAGTAGCTTTCTTATCACTCCAATTCTAGGAATGGCAAAAGTGCTTGGTACGATTGGAAGAATCTCCAAAGCAGTACAGTTTTACCATCGTGCAATTTCAATTTTGGAATCCAGCAGAGGCTTTGAGAACGAGGATTTGGTTATACCTTTATTTAGTCTGGGCAATCTTTTGCTCAAAGAAGGAAAAGGCAAGGATGCTGAAGCTTGTTTTGCTAGGATTGTGAACATATATAAAAAGTTATATGGAGAGAAAGATGGAAAAGTTGGAATGGCTATGTGTTCTCTGGCTAATGCAAAGTGTGCAAGAGGGGAAGCAGAAGAAGCTGTCATCTTATATAGAAGAGCTTTGCAGATTATCAAGGATTCAAATTATATGGCTTTAGATGACAGCGTGCTGGAGAAGATGAGGATTGATTTGGCAGAACTACTGCATGTTGTAGGAAGAGGAAAAGAAGGCAGAGAACTGCTAGAAGAGTGCTTGTTGATCAATGAAAAGTCAAAAGGAAAAGAGCATCCTAGCTCAGTGAAGCACCTTGTAAACCTTGCAGCATCGTATTCACGGTCCAAGAATTACGCAGAGGCTGAGCGTTTGTTGCAAATTGGATTGGACATTATGATTAAGGCAGCGGGACCGGATGACCAATCAATTACAGTCCCAATGTTGCATCTTTCTGTCACTCTTTACAATCTAAAACGAGACGAAGATGCCGAGCAACTTGCGTTGGAAGTTTTGCGAATACGGGAAAATGCATTTGGAAAAGATTCTCTTCCTGTTGGTGAGGCTCTAGACTGTTTGGTTTCCATTCAGAGCAGGCTGGGGAAGGATGAAAACGAGGTACTGAAGATGCTGAAGAGAATTCTAAGCATCCAGGAGAAAGAGTTCGGGTTCGAGGGTAAAGAGGTCATTGACACCCTCAAGAAAATTGTGTTCTACATGGACAAACTAGGAATGAAAGCTGAGAAATTTCCACTTCAAAAACGACTGTCCATGCTTCGAATGAAATTCAAGAACCACATGCAATACTAA

Coding sequence (CDS)

ATGCAGTCATTGCAGTTGCAAAAGGGCATCACTTGCTCCTCAGTTTACCTCCAAAAACAAAAATGCAATATCAAGCTTTATGCCGTACCTGTTAGAGCCTTTTCTTGTCACTTTGCCTCAACACCATCTGCTTCAAGTAGAGCTGATATGGGGGGTCAGAGGAAACATATTTCTTCTGCCTTCACTGCTCCAAATGGATATCAAAGGTCTGATCACATGCATTACCGTACTGATGAGAACAGCATGAGTGAATTTGAGGGTCAGTTGGAAGAATTATTCAATGAAGTCAGAATGATGATTATGAGCGGGAGGAAGAATGATGCTGTAGAGTTACTTCAAGCAAATTATGAAGCTGTAAAAGAACAGATGGAATCAGGTGCTAATGGCATTGAACAAGCTGCTGTTCTTGACATCGTGGCTTTGGGGTATATAACTGTTGGAGACTTGAAGTTTGTTGCTTCTATATTGGATATATTGAACAAGGTCGTTGACAGTCTGAAGGACAGCGAACCCTTTCTGGATTCAGTGCTTCTGCATATGGGGAGCATGTACTCAACTTTGAAGAAGTTTGAGAAATCAATATCTGTGTACAAGAGGGCTATTGATATCATAGAGAAGAAAAGTGGAAAAGATAGTAGCTTTCTTATCACTCCAATTCTAGGAATGGCAAAAGTGCTTGGTACGATTGGAAGAATCTCCAAAGCAGTACAGTTTTACCATCGTGCAATTTCAATTTTGGAATCCAGCAGAGGCTTTGAGAACGAGGATTTGGTTATACCTTTATTTAGTCTGGGCAATCTTTTGCTCAAAGAAGGAAAAGGCAAGGATGCTGAAGCTTGTTTTGCTAGGATTGTGAACATATATAAAAAGTTATATGGAGAGAAAGATGGAAAAGTTGGAATGGCTATGTGTTCTCTGGCTAATGCAAAGTGTGCAAGAGGGGAAGCAGAAGAAGCTGTCATCTTATATAGAAGAGCTTTGCAGATTATCAAGGATTCAAATTATATGGCTTTAGATGACAGCGTGCTGGAGAAGATGAGGATTGATTTGGCAGAACTACTGCATGTTGTAGGAAGAGGAAAAGAAGGCAGAGAACTGCTAGAAGAGTGCTTGTTGATCAATGAAAAGTCAAAAGGAAAAGAGCATCCTAGCTCAGTGAAGCACCTTGTAAACCTTGCAGCATCGTATTCACGGTCCAAGAATTACGCAGAGGCTGAGCGTTTGTTGCAAATTGGATTGGACATTATGATTAAGGCAGCGGGACCGGATGACCAATCAATTACAGTCCCAATGTTGCATCTTTCTGTCACTCTTTACAATCTAAAACGAGACGAAGATGCCGAGCAACTTGCGTTGGAAGTTTTGCGAATACGGGAAAATGCATTTGGAAAAGATTCTCTTCCTGTTGGTGAGGCTCTAGACTGTTTGGTTTCCATTCAGAGCAGGCTGGGGAAGGATGAAAACGAGGTACTGAAGATGCTGAAGAGAATTCTAAGCATCCAGGAGAAAGAGTTCGGGTTCGAGGGTAAAGAGGTCATTGACACCCTCAAGAAAATTGTGTTCTACATGGACAAACTAGGAATGAAAGCTGAGAAATTTCCACTTCAAAAACGACTGTCCATGCTTCGAATGAAATTCAAGAACCACATGCAATACTAA

Protein sequence

MQSLQLQKGITCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADMGGQRKHISSAFTAPNGYQRSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYEAVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVLLHMGSMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQFYHRAISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDGKVGMAMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRGKEGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAAGPDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQSRLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQKRLSMLRMKFKNHMQY
Homology
BLAST of Sgr021594 vs. NCBI nr
Match: XP_022995906.1 (nephrocystin-3 isoform X2 [Cucurbita maxima])

HSP 1 Score: 944.5 bits (2440), Expect = 4.1e-271
Identity = 490/551 (88.93%), Postives = 520/551 (94.37%), Query Frame = 0

Query: 2   QSLQLQKGITCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADMGGQRKHISSAF 61
           QSLQLQKGITCSSV LQKQKCNIKLYAVPVRAFSC FAST SASSR D+G QRKHI+SAF
Sbjct: 34  QSLQLQKGITCSSVSLQKQKCNIKLYAVPVRAFSCRFASTGSASSRDDLGSQRKHIASAF 93

Query: 62  TAPNGYQRSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYEAVKE 121
           T PNGYQR+ HMHYRTD NS SEFEGQL+ELFNEVRM+I+S RK+DAVELLQANYEAVKE
Sbjct: 94  TVPNGYQRAGHMHYRTDGNSASEFEGQLDELFNEVRMLIVSRRKSDAVELLQANYEAVKE 153

Query: 122 QMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVLLHMG 181
           QMESGA GIEQAAVLDIVALGYITVGDLKFVASILDILN +VDSLKD+EPFLDSVLLHMG
Sbjct: 154 QMESGACGIEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKDNEPFLDSVLLHMG 213

Query: 182 SMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQFYHR 241
           SMYSTLKK +KS+S YKRAIDIIEKKSGKDSSFLITPILGMAKVLGT G+ +KAV+FYHR
Sbjct: 214 SMYSTLKKLDKSVSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTSGKTTKAVEFYHR 273

Query: 242 AISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDGKVGM 301
           AISILES RGFE+EDLVIPLFSLGNLLLKEGKGKDAE CFARIVNIYKKLYGEKDGKVGM
Sbjct: 274 AISILESIRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFARIVNIYKKLYGEKDGKVGM 333

Query: 302 AMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRGK 361
           AM SLANAKCARGEA+EA+ LYRRALQIIKDSNYMALDDS +EKMRIDLAELLH VGRGK
Sbjct: 334 AMYSLANAKCARGEADEAITLYRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGRGK 393

Query: 362 EGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAAG 421
           EGRELLEECLLINEKSKGK+HPSSVKHLVNLAASYSRSKNYAEAERLL+IGLDIM+KA G
Sbjct: 394 EGRELLEECLLINEKSKGKDHPSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKAVG 453

Query: 422 PDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQS 481
           PDDQSIT PML+L+VTLYNLKRD+DAEQLALEVLRIRENAFGKD LPVGEALDCLVSIQS
Sbjct: 454 PDDQSITNPMLNLAVTLYNLKRDDDAEQLALEVLRIRENAFGKDCLPVGEALDCLVSIQS 513

Query: 482 RLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQKRLSM 541
           RLGKDE E+LK+LKRIL IQEK FG+E KEVIDTLKKIVFYMDKLG+K EKFP+QKRLS+
Sbjct: 514 RLGKDETELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGLKDEKFPVQKRLSL 573

Query: 542 LRMKFKNHMQY 553
           LRMKFKN MQY
Sbjct: 574 LRMKFKNQMQY 584

BLAST of Sgr021594 vs. NCBI nr
Match: XP_023522378.1 (nephrocystin-3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 943.3 bits (2437), Expect = 9.1e-271
Identity = 491/552 (88.95%), Postives = 520/552 (94.20%), Query Frame = 0

Query: 1   MQSLQLQKGITCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADMGGQRKHISSA 60
           MQSLQLQKG TCSSV LQKQKCNIKLYAVPVRAFSC FAST SASSRAD+G QRKHI+SA
Sbjct: 8   MQSLQLQKGTTCSSVSLQKQKCNIKLYAVPVRAFSCRFASTGSASSRADLGSQRKHIASA 67

Query: 61  FTAPNGYQRSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYEAVK 120
           FT PNGYQR+ HMHYRTD NS SEFEGQL+ELFNEVRM+I+SGRKNDAVELLQANYEAVK
Sbjct: 68  FTVPNGYQRAGHMHYRTDGNSTSEFEGQLDELFNEVRMLIVSGRKNDAVELLQANYEAVK 127

Query: 121 EQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVLLHM 180
           EQMESGA+GIEQAAVLDIVALGYITVGDLKFVASILDILN +VDSLKDSEPFLDSVLLHM
Sbjct: 128 EQMESGASGIEQAAVLDIVALGYITVGDLKFVASILDILNSIVDSLKDSEPFLDSVLLHM 187

Query: 181 GSMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQFYH 240
           GSMYSTLKK EKS+S YKRAIDIIEKKSG+DSSFLITPILGMAKVLGT GR +KAV+ YH
Sbjct: 188 GSMYSTLKKLEKSVSAYKRAIDIIEKKSGQDSSFLITPILGMAKVLGTNGRTTKAVECYH 247

Query: 241 RAISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDGKVG 300
           RAISILES+RGFE+EDLVIPLFSLGNLLLKEGKGKDAE CFARIVNIYKKLYGEKDGKVG
Sbjct: 248 RAISILESTRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFARIVNIYKKLYGEKDGKVG 307

Query: 301 MAMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRG 360
           MAM SLANAKCARGEA+EA+ LYRRALQII DSNYMALDDS +EKMRIDLAELLH VGR 
Sbjct: 308 MAMYSLANAKCARGEADEAITLYRRALQIINDSNYMALDDSEMEKMRIDLAELLHAVGRR 367

Query: 361 KEGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAA 420
           KEGRELLEE LLINEKSKGK+HPSSVKHLVNLAASYSRSKNYAEAERLL+IGL+IM+KA 
Sbjct: 368 KEGRELLEESLLINEKSKGKDHPSSVKHLVNLAASYSRSKNYAEAERLLRIGLNIMVKAV 427

Query: 421 GPDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQ 480
           GPDDQSIT PML+L+VTLYNLKRD+DAEQLALEVLRIRENAFGKD LPVGEALDCLVSIQ
Sbjct: 428 GPDDQSITNPMLNLAVTLYNLKRDDDAEQLALEVLRIRENAFGKDCLPVGEALDCLVSIQ 487

Query: 481 SRLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQKRLS 540
           SRLGKDE E+LK+LKRIL IQEK FG+E KEVIDTLKKIVFYMDKLG+K EKFP+QKRLS
Sbjct: 488 SRLGKDETELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGLKDEKFPVQKRLS 547

Query: 541 MLRMKFKNHMQY 553
           MLRMKFKN MQY
Sbjct: 548 MLRMKFKNQMQY 559

BLAST of Sgr021594 vs. NCBI nr
Match: XP_022958167.1 (nephrocystin-3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 943.0 bits (2436), Expect = 1.2e-270
Identity = 490/551 (88.93%), Postives = 519/551 (94.19%), Query Frame = 0

Query: 2   QSLQLQKGITCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADMGGQRKHISSAF 61
           QSLQLQKGITCSSV LQKQKCNIKLYAVPVRAFSC FAST SASSRAD+G QRKHI+SAF
Sbjct: 34  QSLQLQKGITCSSVSLQKQKCNIKLYAVPVRAFSCQFASTGSASSRADLGSQRKHIASAF 93

Query: 62  TAPNGYQRSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYEAVKE 121
           T PNGYQR  HMHYRTD  S SEFEGQL+ELFNEVRM+I+SGRK+DAVELLQANYEAVKE
Sbjct: 94  TVPNGYQRPGHMHYRTDGTSTSEFEGQLDELFNEVRMLIVSGRKSDAVELLQANYEAVKE 153

Query: 122 QMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVLLHMG 181
           QMESGA GIEQAAVLDIVALGYITVGDLKFVASILDILN +VDSLKD+EPFLDSVLLHMG
Sbjct: 154 QMESGAIGIEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKDNEPFLDSVLLHMG 213

Query: 182 SMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQFYHR 241
           SMYSTLKK EKS+S YKRAIDIIEKKSGKDSSFLITPILGMAKVLGT G+ +KAV+ YHR
Sbjct: 214 SMYSTLKKLEKSMSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTSGKTTKAVESYHR 273

Query: 242 AISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDGKVGM 301
           AISILES+RGFE+EDLVIPLFSLGNLLLKEGKGKDAE CFARIVNIYKKLYGEKDGKVGM
Sbjct: 274 AISILESTRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFARIVNIYKKLYGEKDGKVGM 333

Query: 302 AMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRGK 361
           AM SLANAKCARGEA+EA+ LYRRALQIIKDSNYMALDDS +EKMRIDLAELLH VGRGK
Sbjct: 334 AMYSLANAKCARGEADEAITLYRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGRGK 393

Query: 362 EGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAAG 421
           EGRELLEECLLINEKSKGK+HPSSVKHLVNLAASYSRSKNYAEAERLL+IGLDIM+KA G
Sbjct: 394 EGRELLEECLLINEKSKGKDHPSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKAVG 453

Query: 422 PDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQS 481
           PDDQSIT PML+L+VTLYNLKRD+DAEQLALEVLRIRENAFGKD LPVGEALDCLVSIQS
Sbjct: 454 PDDQSITNPMLNLAVTLYNLKRDDDAEQLALEVLRIRENAFGKDCLPVGEALDCLVSIQS 513

Query: 482 RLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQKRLSM 541
           RLGKD+ E+LK+LKRIL IQEK FG+E KEVIDTLKKIVFYMDKLGMK EKFP+QKRLS+
Sbjct: 514 RLGKDDTELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGMKDEKFPVQKRLSL 573

Query: 542 LRMKFKNHMQY 553
           LR KFKN MQY
Sbjct: 574 LRTKFKNQMQY 584

BLAST of Sgr021594 vs. NCBI nr
Match: XP_022159305.1 (uncharacterized protein LOC111025715 isoform X2 [Momordica charantia] >XP_022159325.1 uncharacterized protein LOC111025733 isoform X2 [Momordica charantia])

HSP 1 Score: 942.6 bits (2435), Expect = 1.6e-270
Identity = 494/555 (89.01%), Postives = 523/555 (94.23%), Query Frame = 0

Query: 1   MQSLQLQKGI--TCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADM-GGQRKHI 60
           MQSLQLQKGI  TC+SV LQ+QKCN+KLYAVPVRAFSCH +STPSASSRAD+  G+RKH+
Sbjct: 24  MQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADLRWGRRKHV 83

Query: 61  SSAFTAPNGYQRSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYE 120
            S FTAPNGYQ + H+HYRTDEN++SE E QLEELFNEVRMMIMSGRKNDAVELL+ANYE
Sbjct: 84  PSTFTAPNGYQSTGHVHYRTDENNISELESQLEELFNEVRMMIMSGRKNDAVELLEANYE 143

Query: 121 AVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVL 180
           AVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVD+LKD EPFLDSVL
Sbjct: 144 AVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKDGEPFLDSVL 203

Query: 181 LHMGSMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQ 240
           LHMGSMYSTLKKFEKS+SVYKRAIDIIEKKSGKDSS LITPILGMAKV+GTIGR SKAVQ
Sbjct: 204 LHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGTIGRTSKAVQ 263

Query: 241 FYHRAISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDG 300
           FYHRAISILESSRGFENEDLVIPLF+LG+LLLKEGKGKDAEACFARIVNIYK LYGEKDG
Sbjct: 264 FYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARIVNIYKNLYGEKDG 323

Query: 301 KVGMAMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVV 360
           KVGMAM SLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSV+EKMRIDLAELLHVV
Sbjct: 324 KVGMAMYSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVV 383

Query: 361 GRGKEGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMI 420
           GRG EGRELLEECLLINEKSKGK+ PSSVKHLVNLA+SYSRSKNYAEAERLL++GL+IM+
Sbjct: 384 GRGNEGRELLEECLLINEKSKGKDDPSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMV 443

Query: 421 KAAGPDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLV 480
           KAAG DD+SITVPMLHL+VTLYNL RDEDAEQLALE LRIRE AFGKD LPVGEALDCLV
Sbjct: 444 KAAGADDESITVPMLHLAVTLYNLNRDEDAEQLALEALRIREIAFGKDCLPVGEALDCLV 503

Query: 481 SIQSRLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQK 540
            IQSR+GKDE E+L ML RIL IQEKEFG EGKEVIDTLKKIVFYMDKLG+K EKFPLQK
Sbjct: 504 CIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFPLQK 563

Query: 541 RLSMLRMKFKNHMQY 553
           RLSMLRMKFKN MQY
Sbjct: 564 RLSMLRMKFKNQMQY 578

BLAST of Sgr021594 vs. NCBI nr
Match: XP_022995907.1 (nephrocystin-3 isoform X3 [Cucurbita maxima] >XP_022995908.1 nephrocystin-3 isoform X3 [Cucurbita maxima])

HSP 1 Score: 941.8 bits (2433), Expect = 2.7e-270
Identity = 491/553 (88.79%), Postives = 521/553 (94.21%), Query Frame = 0

Query: 1   MQSLQLQKGITCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADMGGQRKHISSA 60
           MQSLQLQKGITCSSV LQKQKCNIKLYAVPVRAFSC FAST SASSR D+G QRKHI+SA
Sbjct: 24  MQSLQLQKGITCSSVSLQKQKCNIKLYAVPVRAFSCRFASTGSASSRDDLGSQRKHIASA 83

Query: 61  FTAPNGYQ-RSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYEAV 120
           FT PNGYQ R+ HMHYRTD NS SEFEGQL+ELFNEVRM+I+S RK+DAVELLQANYEAV
Sbjct: 84  FTVPNGYQSRAGHMHYRTDGNSASEFEGQLDELFNEVRMLIVSRRKSDAVELLQANYEAV 143

Query: 121 KEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVLLH 180
           KEQMESGA GIEQAAVLDIVALGYITVGDLKFVASILDILN +VDSLKD+EPFLDSVLLH
Sbjct: 144 KEQMESGACGIEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKDNEPFLDSVLLH 203

Query: 181 MGSMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQFY 240
           MGSMYSTLKK +KS+S YKRAIDIIEKKSGKDSSFLITPILGMAKVLGT G+ +KAV+FY
Sbjct: 204 MGSMYSTLKKLDKSVSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTSGKTTKAVEFY 263

Query: 241 HRAISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDGKV 300
           HRAISILES RGFE+EDLVIPLFSLGNLLLKEGKGKDAE CFARIVNIYKKLYGEKDGKV
Sbjct: 264 HRAISILESIRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFARIVNIYKKLYGEKDGKV 323

Query: 301 GMAMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGR 360
           GMAM SLANAKCARGEA+EA+ LYRRALQIIKDSNYMALDDS +EKMRIDLAELLH VGR
Sbjct: 324 GMAMYSLANAKCARGEADEAITLYRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGR 383

Query: 361 GKEGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKA 420
           GKEGRELLEECLLINEKSKGK+HPSSVKHLVNLAASYSRSKNYAEAERLL+IGLDIM+KA
Sbjct: 384 GKEGRELLEECLLINEKSKGKDHPSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKA 443

Query: 421 AGPDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSI 480
            GPDDQSIT PML+L+VTLYNLKRD+DAEQLALEVLRIRENAFGKD LPVGEALDCLVSI
Sbjct: 444 VGPDDQSITNPMLNLAVTLYNLKRDDDAEQLALEVLRIRENAFGKDCLPVGEALDCLVSI 503

Query: 481 QSRLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQKRL 540
           QSRLGKDE E+LK+LKRIL IQEK FG+E KEVIDTLKKIVFYMDKLG+K EKFP+QKRL
Sbjct: 504 QSRLGKDETELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGLKDEKFPVQKRL 563

Query: 541 SMLRMKFKNHMQY 553
           S+LRMKFKN MQY
Sbjct: 564 SLLRMKFKNQMQY 576

BLAST of Sgr021594 vs. ExPASy Swiss-Prot
Match: Q6AZT7 (Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.4e-11
Identity = 52/213 (24.41%), Postives = 105/213 (49.30%), Query Frame = 0

Query: 121  EQMESGANGIEQAAVLDIVALGYITVGDLK----FVASILDILNKVVDSLKDSEPFLDSV 180
            E++  G +  + A  L+ + + Y    +L+    F+   L++  +V+ +     P     
Sbjct: 1054 EELTLGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMRERVLGA---DHPDCAQS 1113

Query: 181  LLHMGSMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAV 240
            + ++ ++Y+  K+++K+  +Y+RA+DI  +    D   L   +  +A +    G++ KAV
Sbjct: 1114 INNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVKHLAVLYKRKGKLDKAV 1173

Query: 241  QFYHRAISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKD 300
              Y  A+ I + S G ++  +   L +L  L  +  K  DA   + R + IY+   G   
Sbjct: 1174 PLYELAVDIRQKSFGPKHPSVATALVNLAVLYCQMKKQDDALPLYERAMKIYEDSLGRMH 1233

Query: 301  GKVGMAMCSLANAKCARGEAEEAVILYRRALQI 330
             +VG  + +LA  +   G+ E+A  LY+RA++I
Sbjct: 1234 PRVGETLKNLAVLRYEEGDYEKAAELYKRAMEI 1263

BLAST of Sgr021594 vs. ExPASy Swiss-Prot
Match: A0JM23 (Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 3.1e-11
Identity = 51/213 (23.94%), Postives = 105/213 (49.30%), Query Frame = 0

Query: 121  EQMESGANGIEQAAVLDIVALGYITVGDLK----FVASILDILNKVVDSLKDSEPFLDSV 180
            E++  G +  + A  L+ + + Y    +L+    F+   L++  +V+ +     P     
Sbjct: 1065 EELTLGKDTSDNARTLNELGVLYYLQNNLETAETFLKRSLEMRERVLGA---DHPDCAQS 1124

Query: 181  LLHMGSMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAV 240
            + ++ ++Y+  K+++K+  +Y+RA+DI  +    D   L   +  +A +    G++ KAV
Sbjct: 1125 INNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVKHLAVLYKRKGKLDKAV 1184

Query: 241  QFYHRAISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKD 300
              Y  A+ I + S G ++  +   L +L  L  +  K  +A   + R + IY+   G   
Sbjct: 1185 PLYELAVEIRQKSFGPKHPSVATALVNLAVLYCQMKKQAEASPLYERAMKIYEDSLGRMH 1244

Query: 301  GKVGMAMCSLANAKCARGEAEEAVILYRRALQI 330
             +VG  + +LA  +   G+ E+A  LY+RA++I
Sbjct: 1245 PRVGETLKNLAVLRYEEGDFEKAAELYKRAMEI 1274

BLAST of Sgr021594 vs. ExPASy Swiss-Prot
Match: Q07866 (Kinesin light chain 1 OS=Homo sapiens OX=9606 GN=KLC1 PE=1 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 8.9e-11
Identity = 54/195 (27.69%), Postives = 89/195 (45.64%), Query Frame = 0

Query: 312 ARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRGKEGRELLEECL 371
           ++G  E AV L ++AL+ ++ ++    D   +  M   LA +     + K+   LL + L
Sbjct: 225 SQGRYEVAVPLCKQALEDLEKTS--GHDHPDVATMLNILALVYRDQNKYKDAANLLNDAL 284

Query: 372 LINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAAGPDDQSITVPM 431
            I EK+ GK+HP+    L NLA  Y +   Y EAE L +  L+I  K  G D   +   +
Sbjct: 285 AIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQL 344

Query: 432 LHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQSRLGKDENEVL 491
            +L++   N  + E+ E      L I +   G D   V +  + L S   + GK +    
Sbjct: 345 NNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAET 404

Query: 492 KMLKRILSIQEKEFG 507
              + +    E+EFG
Sbjct: 405 LYKEILTRAHEREFG 417

BLAST of Sgr021594 vs. ExPASy Swiss-Prot
Match: Q5R581 (Kinesin light chain 1 OS=Pongo abelii OX=9601 GN=KLC1 PE=2 SV=3)

HSP 1 Score: 70.1 bits (170), Expect = 8.9e-11
Identity = 54/195 (27.69%), Postives = 89/195 (45.64%), Query Frame = 0

Query: 312 ARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRGKEGRELLEECL 371
           ++G  E AV L ++AL+ ++ ++    D   +  M   LA +     + K+   LL + L
Sbjct: 225 SQGRYEVAVPLCKQALEDLEKTS--GHDHPDVATMLNILALVYRDQNKYKDAANLLNDAL 284

Query: 372 LINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAAGPDDQSITVPM 431
            I EK+ GK+HP+    L NLA  Y +   Y EAE L +  L+I  K  G D   +   +
Sbjct: 285 AIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQL 344

Query: 432 LHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQSRLGKDENEVL 491
            +L++   N  + E+ E      L I +   G D   V +  + L S   + GK +    
Sbjct: 345 NNLALLCQNQGKYEEVEYYYQRALEIYQTKLGPDDPNVAKTKNNLASCYLKQGKFKQAET 404

Query: 492 KMLKRILSIQEKEFG 507
              + +    E+EFG
Sbjct: 405 LYKEILTRAHEREFG 417

BLAST of Sgr021594 vs. ExPASy Swiss-Prot
Match: P46825 (Kinesin light chain OS=Doryteuthis pealeii OX=1051067 PE=2 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 8.9e-11
Identity = 55/195 (28.21%), Postives = 90/195 (46.15%), Query Frame = 0

Query: 312 ARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRGKEGRELLEECL 371
           ++G  E AV L ++AL+ ++ ++    D   +  M   LA +    G+ KE   LL + L
Sbjct: 232 SQGRYEVAVPLCKQALEDLEKTS--GHDHPDVATMLNILALVYRDQGKYKEAANLLNDAL 291

Query: 372 LINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAAGPDDQSITVPM 431
            I EK+ G +HP+    L NLA  Y +   Y +AE L +  L I  K  G D   +   +
Sbjct: 292 GIREKTLGPDHPAVAATLNNLAVLYGKRGKYKDAEPLCKRALVIREKVLGKDHPDVAKQL 351

Query: 432 LHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQSRLGKDENEVL 491
            +L++   N  + E+ E+     L I +   G D   V +  + L S   + GK +   +
Sbjct: 352 NNLALLCQNQGKYEEVERYYQRALEIYQKELGPDDPNVAKTKNNLASAYLKQGKYKQAEI 411

Query: 492 KMLKRILSIQEKEFG 507
              + +    EKEFG
Sbjct: 412 LYKEVLTRAHEKEFG 424

BLAST of Sgr021594 vs. ExPASy TrEMBL
Match: A0A6J1K372 (nephrocystin-3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491290 PE=4 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.0e-271
Identity = 490/551 (88.93%), Postives = 520/551 (94.37%), Query Frame = 0

Query: 2   QSLQLQKGITCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADMGGQRKHISSAF 61
           QSLQLQKGITCSSV LQKQKCNIKLYAVPVRAFSC FAST SASSR D+G QRKHI+SAF
Sbjct: 34  QSLQLQKGITCSSVSLQKQKCNIKLYAVPVRAFSCRFASTGSASSRDDLGSQRKHIASAF 93

Query: 62  TAPNGYQRSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYEAVKE 121
           T PNGYQR+ HMHYRTD NS SEFEGQL+ELFNEVRM+I+S RK+DAVELLQANYEAVKE
Sbjct: 94  TVPNGYQRAGHMHYRTDGNSASEFEGQLDELFNEVRMLIVSRRKSDAVELLQANYEAVKE 153

Query: 122 QMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVLLHMG 181
           QMESGA GIEQAAVLDIVALGYITVGDLKFVASILDILN +VDSLKD+EPFLDSVLLHMG
Sbjct: 154 QMESGACGIEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKDNEPFLDSVLLHMG 213

Query: 182 SMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQFYHR 241
           SMYSTLKK +KS+S YKRAIDIIEKKSGKDSSFLITPILGMAKVLGT G+ +KAV+FYHR
Sbjct: 214 SMYSTLKKLDKSVSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTSGKTTKAVEFYHR 273

Query: 242 AISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDGKVGM 301
           AISILES RGFE+EDLVIPLFSLGNLLLKEGKGKDAE CFARIVNIYKKLYGEKDGKVGM
Sbjct: 274 AISILESIRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFARIVNIYKKLYGEKDGKVGM 333

Query: 302 AMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRGK 361
           AM SLANAKCARGEA+EA+ LYRRALQIIKDSNYMALDDS +EKMRIDLAELLH VGRGK
Sbjct: 334 AMYSLANAKCARGEADEAITLYRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGRGK 393

Query: 362 EGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAAG 421
           EGRELLEECLLINEKSKGK+HPSSVKHLVNLAASYSRSKNYAEAERLL+IGLDIM+KA G
Sbjct: 394 EGRELLEECLLINEKSKGKDHPSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKAVG 453

Query: 422 PDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQS 481
           PDDQSIT PML+L+VTLYNLKRD+DAEQLALEVLRIRENAFGKD LPVGEALDCLVSIQS
Sbjct: 454 PDDQSITNPMLNLAVTLYNLKRDDDAEQLALEVLRIRENAFGKDCLPVGEALDCLVSIQS 513

Query: 482 RLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQKRLSM 541
           RLGKDE E+LK+LKRIL IQEK FG+E KEVIDTLKKIVFYMDKLG+K EKFP+QKRLS+
Sbjct: 514 RLGKDETELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGLKDEKFPVQKRLSL 573

Query: 542 LRMKFKNHMQY 553
           LRMKFKN MQY
Sbjct: 574 LRMKFKNQMQY 584

BLAST of Sgr021594 vs. ExPASy TrEMBL
Match: A0A6J1H150 (nephrocystin-3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459474 PE=4 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 5.8e-271
Identity = 490/551 (88.93%), Postives = 519/551 (94.19%), Query Frame = 0

Query: 2   QSLQLQKGITCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADMGGQRKHISSAF 61
           QSLQLQKGITCSSV LQKQKCNIKLYAVPVRAFSC FAST SASSRAD+G QRKHI+SAF
Sbjct: 34  QSLQLQKGITCSSVSLQKQKCNIKLYAVPVRAFSCQFASTGSASSRADLGSQRKHIASAF 93

Query: 62  TAPNGYQRSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYEAVKE 121
           T PNGYQR  HMHYRTD  S SEFEGQL+ELFNEVRM+I+SGRK+DAVELLQANYEAVKE
Sbjct: 94  TVPNGYQRPGHMHYRTDGTSTSEFEGQLDELFNEVRMLIVSGRKSDAVELLQANYEAVKE 153

Query: 122 QMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVLLHMG 181
           QMESGA GIEQAAVLDIVALGYITVGDLKFVASILDILN +VDSLKD+EPFLDSVLLHMG
Sbjct: 154 QMESGAIGIEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKDNEPFLDSVLLHMG 213

Query: 182 SMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQFYHR 241
           SMYSTLKK EKS+S YKRAIDIIEKKSGKDSSFLITPILGMAKVLGT G+ +KAV+ YHR
Sbjct: 214 SMYSTLKKLEKSMSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTSGKTTKAVESYHR 273

Query: 242 AISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDGKVGM 301
           AISILES+RGFE+EDLVIPLFSLGNLLLKEGKGKDAE CFARIVNIYKKLYGEKDGKVGM
Sbjct: 274 AISILESTRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFARIVNIYKKLYGEKDGKVGM 333

Query: 302 AMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRGK 361
           AM SLANAKCARGEA+EA+ LYRRALQIIKDSNYMALDDS +EKMRIDLAELLH VGRGK
Sbjct: 334 AMYSLANAKCARGEADEAITLYRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGRGK 393

Query: 362 EGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAAG 421
           EGRELLEECLLINEKSKGK+HPSSVKHLVNLAASYSRSKNYAEAERLL+IGLDIM+KA G
Sbjct: 394 EGRELLEECLLINEKSKGKDHPSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKAVG 453

Query: 422 PDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQS 481
           PDDQSIT PML+L+VTLYNLKRD+DAEQLALEVLRIRENAFGKD LPVGEALDCLVSIQS
Sbjct: 454 PDDQSITNPMLNLAVTLYNLKRDDDAEQLALEVLRIRENAFGKDCLPVGEALDCLVSIQS 513

Query: 482 RLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQKRLSM 541
           RLGKD+ E+LK+LKRIL IQEK FG+E KEVIDTLKKIVFYMDKLGMK EKFP+QKRLS+
Sbjct: 514 RLGKDDTELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGMKDEKFPVQKRLSL 573

Query: 542 LRMKFKNHMQY 553
           LR KFKN MQY
Sbjct: 574 LRTKFKNQMQY 584

BLAST of Sgr021594 vs. ExPASy TrEMBL
Match: A0A6J1DYD7 (uncharacterized protein LOC111025715 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111025733 PE=4 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 7.5e-271
Identity = 494/555 (89.01%), Postives = 523/555 (94.23%), Query Frame = 0

Query: 1   MQSLQLQKGI--TCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADM-GGQRKHI 60
           MQSLQLQKGI  TC+SV LQ+QKCN+KLYAVPVRAFSCH +STPSASSRAD+  G+RKH+
Sbjct: 24  MQSLQLQKGIKFTCTSVCLQRQKCNVKLYAVPVRAFSCHSSSTPSASSRADLRWGRRKHV 83

Query: 61  SSAFTAPNGYQRSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYE 120
            S FTAPNGYQ + H+HYRTDEN++SE E QLEELFNEVRMMIMSGRKNDAVELL+ANYE
Sbjct: 84  PSTFTAPNGYQSTGHVHYRTDENNISELESQLEELFNEVRMMIMSGRKNDAVELLEANYE 143

Query: 121 AVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVL 180
           AVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVD+LKD EPFLDSVL
Sbjct: 144 AVKEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDNLKDGEPFLDSVL 203

Query: 181 LHMGSMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQ 240
           LHMGSMYSTLKKFEKS+SVYKRAIDIIEKKSGKDSS LITPILGMAKV+GTIGR SKAVQ
Sbjct: 204 LHMGSMYSTLKKFEKSMSVYKRAIDIIEKKSGKDSSLLITPILGMAKVIGTIGRTSKAVQ 263

Query: 241 FYHRAISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDG 300
           FYHRAISILESSRGFENEDLVIPLF+LG+LLLKEGKGKDAEACFARIVNIYK LYGEKDG
Sbjct: 264 FYHRAISILESSRGFENEDLVIPLFNLGDLLLKEGKGKDAEACFARIVNIYKNLYGEKDG 323

Query: 301 KVGMAMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVV 360
           KVGMAM SLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSV+EKMRIDLAELLHVV
Sbjct: 324 KVGMAMYSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVMEKMRIDLAELLHVV 383

Query: 361 GRGKEGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMI 420
           GRG EGRELLEECLLINEKSKGK+ PSSVKHLVNLA+SYSRSKNYAEAERLL++GL+IM+
Sbjct: 384 GRGNEGRELLEECLLINEKSKGKDDPSSVKHLVNLASSYSRSKNYAEAERLLRMGLNIMV 443

Query: 421 KAAGPDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLV 480
           KAAG DD+SITVPMLHL+VTLYNL RDEDAEQLALE LRIRE AFGKD LPVGEALDCLV
Sbjct: 444 KAAGADDESITVPMLHLAVTLYNLNRDEDAEQLALEALRIREIAFGKDCLPVGEALDCLV 503

Query: 481 SIQSRLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQK 540
            IQSR+GKDE E+L ML RIL IQEKEFG EGKEVIDTLKKIVFYMDKLG+K EKFPLQK
Sbjct: 504 CIQSRVGKDEKELLNMLMRILRIQEKEFGMEGKEVIDTLKKIVFYMDKLGIKDEKFPLQK 563

Query: 541 RLSMLRMKFKNHMQY 553
           RLSMLRMKFKN MQY
Sbjct: 564 RLSMLRMKFKNQMQY 578

BLAST of Sgr021594 vs. ExPASy TrEMBL
Match: A0A6J1K9C6 (nephrocystin-3 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111491290 PE=4 SV=1)

HSP 1 Score: 941.8 bits (2433), Expect = 1.3e-270
Identity = 491/553 (88.79%), Postives = 521/553 (94.21%), Query Frame = 0

Query: 1   MQSLQLQKGITCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADMGGQRKHISSA 60
           MQSLQLQKGITCSSV LQKQKCNIKLYAVPVRAFSC FAST SASSR D+G QRKHI+SA
Sbjct: 24  MQSLQLQKGITCSSVSLQKQKCNIKLYAVPVRAFSCRFASTGSASSRDDLGSQRKHIASA 83

Query: 61  FTAPNGYQ-RSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYEAV 120
           FT PNGYQ R+ HMHYRTD NS SEFEGQL+ELFNEVRM+I+S RK+DAVELLQANYEAV
Sbjct: 84  FTVPNGYQSRAGHMHYRTDGNSASEFEGQLDELFNEVRMLIVSRRKSDAVELLQANYEAV 143

Query: 121 KEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVLLH 180
           KEQMESGA GIEQAAVLDIVALGYITVGDLKFVASILDILN +VDSLKD+EPFLDSVLLH
Sbjct: 144 KEQMESGACGIEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKDNEPFLDSVLLH 203

Query: 181 MGSMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQFY 240
           MGSMYSTLKK +KS+S YKRAIDIIEKKSGKDSSFLITPILGMAKVLGT G+ +KAV+FY
Sbjct: 204 MGSMYSTLKKLDKSVSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTSGKTTKAVEFY 263

Query: 241 HRAISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDGKV 300
           HRAISILES RGFE+EDLVIPLFSLGNLLLKEGKGKDAE CFARIVNIYKKLYGEKDGKV
Sbjct: 264 HRAISILESIRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFARIVNIYKKLYGEKDGKV 323

Query: 301 GMAMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGR 360
           GMAM SLANAKCARGEA+EA+ LYRRALQIIKDSNYMALDDS +EKMRIDLAELLH VGR
Sbjct: 324 GMAMYSLANAKCARGEADEAITLYRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGR 383

Query: 361 GKEGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKA 420
           GKEGRELLEECLLINEKSKGK+HPSSVKHLVNLAASYSRSKNYAEAERLL+IGLDIM+KA
Sbjct: 384 GKEGRELLEECLLINEKSKGKDHPSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKA 443

Query: 421 AGPDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSI 480
            GPDDQSIT PML+L+VTLYNLKRD+DAEQLALEVLRIRENAFGKD LPVGEALDCLVSI
Sbjct: 444 VGPDDQSITNPMLNLAVTLYNLKRDDDAEQLALEVLRIRENAFGKDCLPVGEALDCLVSI 503

Query: 481 QSRLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQKRL 540
           QSRLGKDE E+LK+LKRIL IQEK FG+E KEVIDTLKKIVFYMDKLG+K EKFP+QKRL
Sbjct: 504 QSRLGKDETELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGLKDEKFPVQKRL 563

Query: 541 SMLRMKFKNHMQY 553
           S+LRMKFKN MQY
Sbjct: 564 SLLRMKFKNQMQY 576

BLAST of Sgr021594 vs. ExPASy TrEMBL
Match: A0A6J1H2E0 (nephrocystin-3 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111459474 PE=4 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.7e-270
Identity = 491/553 (88.79%), Postives = 520/553 (94.03%), Query Frame = 0

Query: 1   MQSLQLQKGITCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADMGGQRKHISSA 60
           MQSLQLQKGITCSSV LQKQKCNIKLYAVPVRAFSC FAST SASSRAD+G QRKHI+SA
Sbjct: 24  MQSLQLQKGITCSSVSLQKQKCNIKLYAVPVRAFSCQFASTGSASSRADLGSQRKHIASA 83

Query: 61  FTAPNGYQ-RSDHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYEAV 120
           FT PNGYQ R  HMHYRTD  S SEFEGQL+ELFNEVRM+I+SGRK+DAVELLQANYEAV
Sbjct: 84  FTVPNGYQSRPGHMHYRTDGTSTSEFEGQLDELFNEVRMLIVSGRKSDAVELLQANYEAV 143

Query: 121 KEQMESGANGIEQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVLLH 180
           KEQMESGA GIEQAAVLDIVALGYITVGDLKFVASILDILN +VDSLKD+EPFLDSVLLH
Sbjct: 144 KEQMESGAIGIEQAAVLDIVALGYITVGDLKFVASILDILNNIVDSLKDNEPFLDSVLLH 203

Query: 181 MGSMYSTLKKFEKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQFY 240
           MGSMYSTLKK EKS+S YKRAIDIIEKKSGKDSSFLITPILGMAKVLGT G+ +KAV+ Y
Sbjct: 204 MGSMYSTLKKLEKSMSAYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTSGKTTKAVESY 263

Query: 241 HRAISILESSRGFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDGKV 300
           HRAISILES+RGFE+EDLVIPLFSLGNLLLKEGKGKDAE CFARIVNIYKKLYGEKDGKV
Sbjct: 264 HRAISILESTRGFEDEDLVIPLFSLGNLLLKEGKGKDAETCFARIVNIYKKLYGEKDGKV 323

Query: 301 GMAMCSLANAKCARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGR 360
           GMAM SLANAKCARGEA+EA+ LYRRALQIIKDSNYMALDDS +EKMRIDLAELLH VGR
Sbjct: 324 GMAMYSLANAKCARGEADEAITLYRRALQIIKDSNYMALDDSEMEKMRIDLAELLHAVGR 383

Query: 361 GKEGRELLEECLLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKA 420
           GKEGRELLEECLLINEKSKGK+HPSSVKHLVNLAASYSRSKNYAEAERLL+IGLDIM+KA
Sbjct: 384 GKEGRELLEECLLINEKSKGKDHPSSVKHLVNLAASYSRSKNYAEAERLLRIGLDIMVKA 443

Query: 421 AGPDDQSITVPMLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSI 480
            GPDDQSIT PML+L+VTLYNLKRD+DAEQLALEVLRIRENAFGKD LPVGEALDCLVSI
Sbjct: 444 VGPDDQSITNPMLNLAVTLYNLKRDDDAEQLALEVLRIRENAFGKDCLPVGEALDCLVSI 503

Query: 481 QSRLGKDENEVLKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQKRL 540
           QSRLGKD+ E+LK+LKRIL IQEK FG+E KEVIDTLKKIVFYMDKLGMK EKFP+QKRL
Sbjct: 504 QSRLGKDDTELLKLLKRILRIQEKAFGYEAKEVIDTLKKIVFYMDKLGMKDEKFPVQKRL 563

Query: 541 SMLRMKFKNHMQY 553
           S+LR KFKN MQY
Sbjct: 564 SLLRTKFKNQMQY 576

BLAST of Sgr021594 vs. TAIR 10
Match: AT5G53080.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 615.9 bits (1587), Expect = 3.1e-176
Identity = 322/542 (59.41%), Postives = 418/542 (77.12%), Query Frame = 0

Query: 11  TCSSVYLQKQKCNIKLYAVPVRAFSCHFASTPSASSRADMGGQRKHISSAFTAPNGYQRS 70
           T   V L+ QK   KLY +P R    HF STP  S  +        I+++  A +G    
Sbjct: 33  TWQCVCLRNQKRKPKLYLIPAR----HFLSTPIDSVSS------SSITASRYATSGVSEV 92

Query: 71  DHMHYRTDENSMSEFEGQLEELFNEVRMMIMSGRKNDAVELLQANYEAVKEQMESGANGI 130
                  +   M EFE +L+ELFNEV+ M+  G+++DA++LL+ANY AVKE+++SG  GI
Sbjct: 93  QRSTSSNNVTEMEEFEMELQELFNEVKSMVKIGKESDAMDLLRANYVAVKEELDSGLKGI 152

Query: 131 EQAAVLDIVALGYITVGDLKFVASILDILNKVVDSLKDSEPFLDSVLLHMGSMYSTLKKF 190
           EQAAVLDI+ALGY+ VGDLK V ++LD++NK+VD+LKDSEP LDSVL+H+GSMYS + KF
Sbjct: 153 EQAAVLDIIALGYMAVGDLKPVPALLDMINKIVDNLKDSEPLLDSVLMHVGSMYSVIGKF 212

Query: 191 EKSISVYKRAIDIIEKKSGKDSSFLITPILGMAKVLGTIGRISKAVQFYHRAISILESSR 250
           E +I V++RAI I+E + GK ++ L+TP+LGMAK   + G+ +KA+  Y R ++ILE +R
Sbjct: 213 ENAILVHQRAIRILENRYGKCNTLLVTPLLGMAKSFASDGKATKAIGVYERTLTILERNR 272

Query: 251 GFENEDLVIPLFSLGNLLLKEGKGKDAEACFARIVNIYKKLYGEKDGKVGMAMCSLANAK 310
           G E+EDLV+PLFSLG LLLKEGK  +AE  F  IVNIYKK+YGE+DG+VGMAMCSLANAK
Sbjct: 273 GSESEDLVVPLFSLGKLLLKEGKAAEAEIPFTSIVNIYKKIYGERDGRVGMAMCSLANAK 332

Query: 311 CARGEAEEAVILYRRALQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRGKEGRELLEEC 370
           C++G+A EAV +YR AL+IIKDSNYM +D+S+LE MRIDLAELLH VGRG EGRELLEEC
Sbjct: 333 CSKGDANEAVDIYRNALRIIKDSNYMTIDNSILENMRIDLAELLHFVGRGDEGRELLEEC 392

Query: 371 LLINEKSKGKEHPSSVKHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAAGPDDQSITVP 430
           LLINE+ KGK HPS   HL+NLAASYSRSKNY EAERLL+  L+IM  + G + QSIT P
Sbjct: 393 LLINERFKGKNHPSMATHLINLAASYSRSKNYVEAERLLRTCLNIMEVSVGSEGQSITFP 452

Query: 431 MLHLSVTLYNLKRDEDAEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQSRLGKDENEV 490
           ML+L+VTL  L RDE+AEQ+AL+VLRIRE AFG+DSLPVGEALDCLVSIQ+RLG+D+ E+
Sbjct: 453 MLNLAVTLSQLNRDEEAEQIALKVLRIREKAFGEDSLPVGEALDCLVSIQARLGRDDGEI 512

Query: 491 LKMLKRILSIQEKEFGFEGKEVIDTLKKIVFYMDKLGMKAEKFPLQKRLSMLRMKFKNHM 550
           L +LKR++ IQEKEFG   +E+I TL+KI+ +++KL MK +KF  ++RL++LR ++K  +
Sbjct: 513 LGLLKRVMMIQEKEFGPSAQELIVTLQKIIHFLEKLEMKDDKFKFRRRLALLRERYKQSL 564

Query: 551 QY 553
            Y
Sbjct: 573 SY 564

BLAST of Sgr021594 vs. TAIR 10
Match: AT4G10840.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 6.6e-09
Identity = 56/292 (19.18%), Postives = 129/292 (44.18%), Query Frame = 0

Query: 208 SGKDSSFLITPILGMAKVLGTIGRISKAVQFYHRAISILESSRGFENEDLVIPLFSLGNL 267
           SG++S      +  +  +  ++ R  +AV  Y +A+++ ++S+G  +  +      L  L
Sbjct: 300 SGQESEVASIDV-SIGNIYMSLCRFDEAVFSYQKALTVFKASKGETHPTVASVFVRLAEL 359

Query: 268 LLKEGKGKDAEACFARIVNIYKK-LYGEKDGKVGMAMCSLANAKCARGEAEEAVILYRRA 327
             + GK +++++     + IY K + G    ++   +  ++    +  E EEA+ L +++
Sbjct: 360 YHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESVDEPEEALKLLQKS 419

Query: 328 LQIIKDSNYMALDDSVLEKMRIDLAELLHVVGRGKEGRELLEECLLINEKSKGKEHPSSV 387
           +++++D        S +  +   +  + + VGR ++ R   E   +   ++ G++     
Sbjct: 420 MKLLEDK---PGQQSAIAGLEARMGVMYYTVGRYEDARNAFESA-VTKLRAAGEKSAFFG 479

Query: 388 KHLVNLAASYSRSKNYAEAERLLQIGLDIMIKAAGPDDQSITVPMLHLSVTLYNLKRDED 447
             L  +  +  +     EA  L +    I+ +  GP DQ       +L+ T   + R ED
Sbjct: 480 VVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAATYDAMGRIED 539

Query: 448 AEQLALEVLRIRENAFGKDSLPVGEALDCLVSIQSRLGKDENEVLKMLKRIL 499
           A ++  +VL++RE   G  +    +    L  +    G+  N   K L+ ++
Sbjct: 540 AIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEAGRSRNYKAKSLQNLI 586

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022995906.14.1e-27188.93nephrocystin-3 isoform X2 [Cucurbita maxima][more]
XP_023522378.19.1e-27188.95nephrocystin-3-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022958167.11.2e-27088.93nephrocystin-3 isoform X2 [Cucurbita moschata][more]
XP_022159305.11.6e-27089.01uncharacterized protein LOC111025715 isoform X2 [Momordica charantia] >XP_022159... [more]
XP_022995907.12.7e-27088.79nephrocystin-3 isoform X3 [Cucurbita maxima] >XP_022995908.1 nephrocystin-3 isof... [more]
Match NameE-valueIdentityDescription
Q6AZT72.4e-1124.41Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1[more]
A0JM233.1e-1123.94Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2[more]
Q078668.9e-1127.69Kinesin light chain 1 OS=Homo sapiens OX=9606 GN=KLC1 PE=1 SV=2[more]
Q5R5818.9e-1127.69Kinesin light chain 1 OS=Pongo abelii OX=9601 GN=KLC1 PE=2 SV=3[more]
P468258.9e-1128.21Kinesin light chain OS=Doryteuthis pealeii OX=1051067 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1K3722.0e-27188.93nephrocystin-3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491290 PE=4 SV=1[more]
A0A6J1H1505.8e-27188.93nephrocystin-3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459474 PE=4 SV=... [more]
A0A6J1DYD77.5e-27189.01uncharacterized protein LOC111025715 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1K9C61.3e-27088.79nephrocystin-3 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111491290 PE=4 SV=1[more]
A0A6J1H2E03.7e-27088.79nephrocystin-3 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111459474 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT5G53080.13.1e-17659.41Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.16.6e-0919.18Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 187..207
NoneNo IPR availablePFAMPF13424TPR_12coord: 176..247
e-value: 3.0E-8
score: 33.8
coord: 346..415
e-value: 2.1E-10
score: 40.8
coord: 263..331
e-value: 4.4E-10
score: 39.7
NoneNo IPR availablePFAMPF13374TPR_10coord: 431..466
e-value: 2.6E-5
score: 23.9
NoneNo IPR availablePANTHERPTHR45641TETRATRICOPEPTIDE REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_6G03870)coord: 89..540
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 258..291
e-value: 15.0
score: 13.4
coord: 300..333
e-value: 1.5
score: 17.9
coord: 386..419
e-value: 49.0
score: 8.9
coord: 216..249
e-value: 33.0
score: 10.4
coord: 174..207
e-value: 10.0
score: 14.9
coord: 344..377
e-value: 480.0
score: 0.2
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 174..207
score: 9.2634
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 344..551
e-value: 1.4E-27
score: 98.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 174..343
e-value: 6.1E-29
score: 102.9
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 159..415
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 348..485

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr021594.1Sgr021594.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0031425 chloroplast RNA processing
cellular_component GO:0009507 chloroplast
molecular_function GO:0005515 protein binding