Spg036546 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg036546
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	kunitz trypsin inhibitor 2
Location	scaffold5: 46200200 .. 46200820 (+)
RNA-Seq Expression	Spg036546
Synteny	Spg036546

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAATTTGCATTACTGTCATTTCTTTTCATCGTCATTGCCTCTACTGAGGTACGCTTGTGCAAAGCCGGAGCCTCGCCGGACGCCGTCCTCGACGTCAACGGAGAGAAGCTCCGAGCCGGCGTCAACTACTCCATCCTGCCGTATTTCGGCAGAAACGCCGGCGGACTAACGATCGGCAGTATCCGGAAGGGATACGATCAATGTCCAGTCAACATCGTCCCAGAAACTTACGAAAGATCCATGGGTCTTCCGGCGACGTTTTCGCCCATAAACCCTAAGAAAGGCGTGGTTCGAGTTTCCACCGATCTGAACATCCAATTTCAGGCGAACGGCAAGTGCGGCAAATCGACGGTCTGGAAAGTAGCTAAATTGGATCAATCCACGGGACAGTATTTCGTGACGATCGGCGGAACCAAAGGAAACCCAGGGCGCGAGACGCTCGACAGTTGGTTCAAAATTGAGAAGCTCGGCAACAATTACAAGTTCGTGCACTGTCCGACTGTGTGTAAGTACTGCAAAGTTATATGCAAAGATGTCAGCATATTCTATGCGAATGGAAGGTTGACTCTTGTTTTGAACGATGCGGCATACCCTGTTATGTTCAAGAAAGTTTAA

mRNA sequence

ATGAAGAAATTTGCATTACTGTCATTTCTTTTCATCGTCATTGCCTCTACTGAGGTACGCTTGTGCAAAGCCGGAGCCTCGCCGGACGCCGTCCTCGACGTCAACGGAGAGAAGCTCCGAGCCGGCGTCAACTACTCCATCCTGCCGTATTTCGGCAGAAACGCCGGCGGACTAACGATCGGCAGTATCCGGAAGGGATACGATCAATGTCCAGTCAACATCGTCCCAGAAACTTACGAAAGATCCATGGGTCTTCCGGCGACGTTTTCGCCCATAAACCCTAAGAAAGGCGTGGTTCGAGTTTCCACCGATCTGAACATCCAATTTCAGGCGAACGGCAAGTGCGGCAAATCGACGGTCTGGAAAGTAGCTAAATTGGATCAATCCACGGGACAGTATTTCGTGACGATCGGCGGAACCAAAGGAAACCCAGGGCGCGAGACGCTCGACAGTTGGTTCAAAATTGAGAAGCTCGGCAACAATTACAAGTTCGTGCACTGTCCGACTGTGTGTAAGTACTGCAAAGTTATATGCAAAGATGTCAGCATATTCTATGCGAATGGAAGGTTGACTCTTGTTTTGAACGATGCGGCATACCCTGTTATGTTCAAGAAAGTTTAA

Coding sequence (CDS)

ATGAAGAAATTTGCATTACTGTCATTTCTTTTCATCGTCATTGCCTCTACTGAGGTACGCTTGTGCAAAGCCGGAGCCTCGCCGGACGCCGTCCTCGACGTCAACGGAGAGAAGCTCCGAGCCGGCGTCAACTACTCCATCCTGCCGTATTTCGGCAGAAACGCCGGCGGACTAACGATCGGCAGTATCCGGAAGGGATACGATCAATGTCCAGTCAACATCGTCCCAGAAACTTACGAAAGATCCATGGGTCTTCCGGCGACGTTTTCGCCCATAAACCCTAAGAAAGGCGTGGTTCGAGTTTCCACCGATCTGAACATCCAATTTCAGGCGAACGGCAAGTGCGGCAAATCGACGGTCTGGAAAGTAGCTAAATTGGATCAATCCACGGGACAGTATTTCGTGACGATCGGCGGAACCAAAGGAAACCCAGGGCGCGAGACGCTCGACAGTTGGTTCAAAATTGAGAAGCTCGGCAACAATTACAAGTTCGTGCACTGTCCGACTGTGTGTAAGTACTGCAAAGTTATATGCAAAGATGTCAGCATATTCTATGCGAATGGAAGGTTGACTCTTGTTTTGAACGATGCGGCATACCCTGTTATGTTCAAGAAAGTTTAA

Protein sequence

MKKFALLSFLFIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTIGSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNNYKFVHCPTVCKYCKVICKDVSIFYANGRLTLVLNDAAYPVMFKKV

Homology

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BLAST of Spg036546 vs. NCBI nr
Match: XP_038895396.1 (miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa hispida])

HSP 1 Score: 314.3 bits (804), Expect = 7.7e-82
Identity = 146/206 (70.87%), Postives = 171/206 (83.01%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTI 60
           MK FALLSFLFIVI S+EVR C+A  SPDAVLD +G+KLR+   Y IL  + RN+GGL+I
Sbjct: 1   MKNFALLSFLFIVIVSSEVRFCRADVSPDAVLDTDGKKLRSSDQYYILSVYSRNSGGLSI 60

Query: 61  GSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTV 120
           GSI++G ++CP+NI+PE+YE   GL ATF PINPKKGVVRVSTDLNIQF+A+ +CG+STV
Sbjct: 61  GSIQQGKEKCPINILPESYEYLHGLAATFFPINPKKGVVRVSTDLNIQFEASTRCGESTV 120

Query: 121 WKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNNYKFVHCPTVCKYCKVICKD 180
           WK+ K DQ   QYFVTIGG KGNPGRET+ +WFKIEK GNNYK VHCPTVCKYCKVICKD
Sbjct: 121 WKIGKFDQYLKQYFVTIGGVKGNPGRETVGNWFKIEKYGNNYKLVHCPTVCKYCKVICKD 180

Query: 181 VSIFYANGRLTLVLNDAAYPVMFKKV 207
           V IFY NG+  L LND+ +PVMFKKV
Sbjct: 181 VGIFYKNGKRVLALNDSPFPVMFKKV 206

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BLAST of Spg036546 vs. NCBI nr
Match: XP_008437082.1 (PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. makuwa])

HSP 1 Score: 312.0 bits (798), Expect = 3.8e-81
Identity = 146/206 (70.87%), Postives = 172/206 (83.50%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTI 60
           MK FALL FLFIVIAS+EVR C+A ASPDAVLD +G+KLRAG  Y IL  + RN+GGL+I
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTV 120
           G I  GY++CP+NI+PE+Y+   GLPATFSP+NPKKGVVRVSTDLNI+F+A+ +CG STV
Sbjct: 61  GGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTV 120

Query: 121 WKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNNYKFVHCPTVCKYCKVICKD 180
           WKV K DQ   QYFVT+GGTKGNPGRET+ +WFK+EK G NYKFV+CPTVCKYCKV+CKD
Sbjct: 121 WKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKD 180

Query: 181 VSIFYANGRLTLVLNDAAYPVMFKKV 207
           V +FY NGR    LNDA +PVMFKKV
Sbjct: 181 VGLFYKNGRRIFALNDAPFPVMFKKV 205

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BLAST of Spg036546 vs. NCBI nr
Match: XP_022137472.1 (kunitz trypsin inhibitor 2 [Momordica charantia])

HSP 1 Score: 292.7 bits (748), Expect = 2.4e-75
Identity = 137/206 (66.50%), Postives = 168/206 (81.55%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTI 60
           MK FALL FLF +IASTEVR+CK+ ASPDAVLD++G+KLRAGVNY ILP      GGLT+
Sbjct: 1   MKNFALLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60

Query: 61  GSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTV 120
           G+I  G+D+CP+++V E +E S GLPATF+P+NPKKGVVRVSTDLN++F+A+  C KSTV
Sbjct: 61  GNI--GHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKSTV 120

Query: 121 WKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNNYKFVHCPTVCKYCKVICKD 180
           WK+ K D+ST Q+ V IGG +GNPG+ETLD+WFKIEK G +YKFV CP+VCK+CKVICKD
Sbjct: 121 WKLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKDYKFVFCPSVCKFCKVICKD 180

Query: 181 VSIFYANGRLTLVLNDAAYPVMFKKV 207
           V IF  NG   L L+D  +PVMFKKV
Sbjct: 181 VGIFIKNGNRALALSDTPFPVMFKKV 204

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BLAST of Spg036546 vs. NCBI nr
Match: XP_038893656.1 (miraculin-like [Benincasa hispida])

HSP 1 Score: 284.3 bits (726), Expect = 8.6e-73
Identity = 137/211 (64.93%), Postives = 168/211 (79.62%), Query Frame = 0


Query: 1   MKKFALLSFLFIVI-ASTE-VRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGL 60
           MK F LLS +FIVI ASTE  + C+  ASP+A+LD NG+KLR G +Y ILP+F RN GGL
Sbjct: 1   MKSFELLSLIFIVIVASTERQQFCRVNASPEAILDTNGKKLRGGDHYYILPFFSRNTGGL 60

Query: 61  TIGSIRKGY-DQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGK 120
            +G+I++ Y ++CP+N+VPE YE   GLP TFSP+NPKKGV+RVSTDLNIQF+ N  C K
Sbjct: 61  ALGNIQREYSNECPLNVVPEPYEYFNGLPTTFSPVNPKKGVIRVSTDLNIQFERNTMCAK 120

Query: 121 STVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNN--YKFVHCPTVCKYCK 180
           STVWK++K +Q   QY VTIGGTKG+PG ET+++WFKIEK  NN  YKFV+CPTVCKYCK
Sbjct: 121 STVWKISKFEQ---QYLVTIGGTKGDPGPETMENWFKIEKYNNNNIYKFVYCPTVCKYCK 180

Query: 181 VICKDVSIFYANGRLTLVLNDAAYPVMFKKV 207
           V+CKDV IF  NGR+ L L+DA +PVMFKKV
Sbjct: 181 VMCKDVGIFVKNGRMALALSDAPFPVMFKKV 208

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BLAST of Spg036546 vs. NCBI nr
Match: XP_008437071.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 279.6 bits (714), Expect = 2.1e-71
Identity = 140/210 (66.67%), Postives = 162/210 (77.14%), Query Frame = 0


Query: 1   MKKFALLSFLF--IVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYF-GRNAGG 60
           MKKFALLSFLF  IVIAS+E+R C+A ASPDAVLD +G+KLR    Y ILP F G   GG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LTIGSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGK 120
           L IG+IRK YD+C +N+V E YE+S G P TF PINPKKGVVRVSTDLNI+F A  +C K
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLG-NNYKFVHCPTVCKYCKV 180
           STVWK+   D+   QYFVTIGGTKGNPGRET ++WFKIEK G  NYK V+CP VCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 ICKDVSIFYANGRLTLVLNDAAYPVMFKKV 207
           +CKD+ IF  NG   LVL+D  +PV+FKKV
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

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BLAST of Spg036546 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 180.6 bits (457), Expect = 1.8e-44
Identity = 102/216 (47.22%), Postives = 127/216 (58.80%), Query Frame = 0


Query: 1   MKKFALLSFLF-----IVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNA 60
           MK+  +LS  F     ++ A+    L  A ++P+ VLD++GEKLR G NY I+P    + 
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 61  GGLTIGSIR-KGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGK 120
           GGLT+ +    G   CP  +V    E     P  F P NPK+ VVRVSTDLNI F A   
Sbjct: 61  GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120

Query: 121 C--GKSTVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNN--YKFVHCPTV 180
           C    STVW++ K D+STGQYFVTIGG KGNPG ET+ SWFKIE+   +  YK V CPTV
Sbjct: 121 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTV 180

Query: 181 CKYCKVICKDVSIFY-ANGRLTLVLNDAAYPVMFKK 206
           C  CKV C DV I+    GR  L L+D  +   F K
Sbjct: 181 CGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

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BLAST of Spg036546 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.5e-40
Identity = 80/200 (40.00%), Postives = 127/200 (63.50%), Query Frame = 0


Query: 7   LSFLFIVIA-STEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTIGSIRK 66
           L ++F+++A     R     A+ + V D+NG+ L  GVNY ILP      GGLT+ +++ 
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 67  GYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTVWKVAK 126
             + CP +++ + +E S GLP  FSP + K   + VSTD+NI+F        +++W++A 
Sbjct: 64  --ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELAN 123

Query: 127 LDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNNYKFVHCPTVCKYCKVICKDVSIFY 186
            D++T Q+F++  G +GNPG++T+D+WFKI+K   +YK   CPTVC +CKVIC+DV +F 
Sbjct: 124 FDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFV 183

Query: 187 ANGRLTLVLNDAAYPVMFKK 206
            +G+  L L+D    VMFK+
Sbjct: 184 QDGKRRLALSDVPLKVMFKR 194

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BLAST of Spg036546 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.2e-31
Identity = 82/210 (39.05%), Postives = 109/210 (51.90%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYF-GRNAGGLT 60
           MK    +  L     S       A A+   VLD +G++L+ GV Y +L    G   GGL 
Sbjct: 1   MKTATAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLA 60

Query: 61  IGSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQ--ANGKCGK 120
           +G  R     CP  +V    +   G P  FS  + K  VVRVSTD+NI+F    +  C  
Sbjct: 61  LG--RATGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCST 120

Query: 121 STVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLG-NNYKFVHCPTVCKYCKV 180
           STVW++   D S G+++VT  G KG PG  TL SWFKIEK G   YKF  CP+VC  C  
Sbjct: 121 STVWRLDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTT 180

Query: 181 ICKDVSIFY-ANGRLTLVLNDAAYPVMFKK 206
           +C D+      +G++ L L+D  +  MFKK
Sbjct: 181 LCSDIGRHSDDDGQIRLALSDNEWAWMFKK 208

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BLAST of Spg036546 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 1.7e-23
Identity = 74/198 (37.37%), Postives = 101/198 (51.01%), Query Frame = 0


Query: 11  FIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTIGSIRKGYDQC 70
           ++V+A T V    A  +  AV+D++G  +    +Y +LP      GGLT+    +G   C
Sbjct: 13  YLVLALTAV---LASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG--RGGQPC 72

Query: 71  PVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGK-CGKSTVWKVAKLDQS 130
           P +IV E+ E   G+P  FS    K   V  S +LNI+       C +ST W+V + D  
Sbjct: 73  PYDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHE 132

Query: 131 TGQYFVTIGGTKGNPGRETLDSWFKIEKLGNN-YKFVHCPTVCKYCKVICKDVSIFYAN- 190
             QYFV  G      G+++L S+FKIEK G + YKFV CP  C      C DV IF    
Sbjct: 133 RKQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDEL 192

Query: 191 GRLTLVLNDAAYPVMFKK 206
           G   L L+D  + VMFKK
Sbjct: 193 GVRRLALSDKPFLVMFKK 204

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BLAST of Spg036546 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.8e-18
Identity = 58/205 (28.29%), Postives = 103/205 (50.24%), Query Frame = 0


Query: 6   LLSFLFIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTIGSIRK 65
           +L+ +++++A+T +           V+D +GE +     Y I P    N GG T+ +   
Sbjct: 10  ILAHVWLLMATTSI--------AQFVIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT--- 69

Query: 66  GYDQCPVNIVPETYERSMGLPATFSPINPK--KGVVRVSTDLNIQFQANGKCGKSTVWKV 125
           G   CP+++  +  E ++G+   F+P  P+     VR++ DL + F  +  CG+ST W++
Sbjct: 70  GNGPCPLHVGLDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRL 129

Query: 126 AKLDQSTGQYFVTIGGTKGNPGRETLDSWFKI--EKLGNNYKFVHCPT-VCKYCKVICKD 185
            + D ++G+  +  G   G   +    ++F+I   + G  Y    CPT  C  CKV C  
Sbjct: 130 GEKDATSGRRLIVTGRDNGAGSQ---GNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGT 189

Query: 186 VSIFYANGRLTLVLNDAAYPVMFKK 206
           V +   NG+  L L+  A PV+F+K
Sbjct: 190 VGVIRENGKNLLALDGDALPVVFQK 200

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BLAST of Spg036546 vs. ExPASy TrEMBL
Match: A0A0A0LT81 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.1e-81
Identity = 145/206 (70.39%), Postives = 172/206 (83.50%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTI 60
           M+ FALL FLFIVIAS+EVR C+A ASPDAVLD +G+KLRAG  Y IL  + RN+GGL+I
Sbjct: 1   MRNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTV 120
           G I  GY++CP+NI+PE+Y+   GLPATFSPINPKKGVVRVSTDLNIQF+AN +CG STV
Sbjct: 61  GGI-YGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGISTV 120

Query: 121 WKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNNYKFVHCPTVCKYCKVICKD 180
           WKV K D+   QYFVT+GG KGNPGRET+++WFK+EK G NYK V+CPTVCKYCKV+CKD
Sbjct: 121 WKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGKNYKLVYCPTVCKYCKVVCKD 180

Query: 181 VSIFYANGRLTLVLNDAAYPVMFKKV 207
           V +FY NGR  + LNDA +PVMFKKV
Sbjct: 181 VGLFYKNGRRVIALNDAPFPVMFKKV 205

[Close]

BLAST of Spg036546 vs. ExPASy TrEMBL
Match: A0A5D3BJ02 (Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.9e-81
Identity = 146/206 (70.87%), Postives = 172/206 (83.50%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTI 60
           MK FALL FLFIVIAS+EVR C+A ASPDAVLD +G+KLRAG  Y IL  + RN+GGL+I
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTV 120
           G I  GY++CP+NI+PE+Y+   GLPATFSP+NPKKGVVRVSTDLNI+F+A+ +CG STV
Sbjct: 61  GGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTV 120

Query: 121 WKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNNYKFVHCPTVCKYCKVICKD 180
           WKV K DQ   QYFVT+GGTKGNPGRET+ +WFK+EK G NYKFV+CPTVCKYCKV+CKD
Sbjct: 121 WKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKD 180

Query: 181 VSIFYANGRLTLVLNDAAYPVMFKKV 207
           V +FY NGR    LNDA +PVMFKKV
Sbjct: 181 VGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of Spg036546 vs. ExPASy TrEMBL
Match: A0A1S3AST3 (miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.9e-81
Identity = 146/206 (70.87%), Postives = 172/206 (83.50%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTI 60
           MK FALL FLFIVIAS+EVR C+A ASPDAVLD +G+KLRAG  Y IL  + RN+GGL+I
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTV 120
           G I  GY++CP+NI+PE+Y+   GLPATFSP+NPKKGVVRVSTDLNI+F+A+ +CG STV
Sbjct: 61  GGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTV 120

Query: 121 WKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNNYKFVHCPTVCKYCKVICKD 180
           WKV K DQ   QYFVT+GGTKGNPGRET+ +WFK+EK G NYKFV+CPTVCKYCKV+CKD
Sbjct: 121 WKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKD 180

Query: 181 VSIFYANGRLTLVLNDAAYPVMFKKV 207
           V +FY NGR    LNDA +PVMFKKV
Sbjct: 181 VGLFYKNGRRIFALNDAPFPVMFKKV 205

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BLAST of Spg036546 vs. ExPASy TrEMBL
Match: A0A6J1C8C4 (kunitz trypsin inhibitor 2 OS=Momordica charantia OX=3673 GN=LOC111008908 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.2e-75
Identity = 137/206 (66.50%), Postives = 168/206 (81.55%), Query Frame = 0


Query: 1   MKKFALLSFLFIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTI 60
           MK FALL FLF +IASTEVR+CK+ ASPDAVLD++G+KLRAGVNY ILP      GGLT+
Sbjct: 1   MKNFALLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60

Query: 61  GSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTV 120
           G+I  G+D+CP+++V E +E S GLPATF+P+NPKKGVVRVSTDLN++F+A+  C KSTV
Sbjct: 61  GNI--GHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKSTV 120

Query: 121 WKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNNYKFVHCPTVCKYCKVICKD 180
           WK+ K D+ST Q+ V IGG +GNPG+ETLD+WFKIEK G +YKFV CP+VCK+CKVICKD
Sbjct: 121 WKLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKDYKFVFCPSVCKFCKVICKD 180

Query: 181 VSIFYANGRLTLVLNDAAYPVMFKKV 207
           V IF  NG   L L+D  +PVMFKKV
Sbjct: 181 VGIFIKNGNRALALSDTPFPVMFKKV 204

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BLAST of Spg036546 vs. ExPASy TrEMBL
Match: A0A5D3BJ25 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002780 PE=3 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 1.0e-71
Identity = 140/210 (66.67%), Postives = 162/210 (77.14%), Query Frame = 0


Query: 1   MKKFALLSFLF--IVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYF-GRNAGG 60
           MKKFALLSFLF  IVIAS+E+R C+A ASPDAVLD +G+KLR    Y ILP F G   GG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LTIGSIRKGYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGK 120
           L IG+IRK YD+C +N+V E YE+S G P TF PINPKKGVVRVSTDLNI+F A  +C K
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKVAKLDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLG-NNYKFVHCPTVCKYCKV 180
           STVWK+   D+   QYFVTIGGTKGNPGRET ++WFKIEK G  NYK V+CP VCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 ICKDVSIFYANGRLTLVLNDAAYPVMFKKV 207
           +CKD+ IF  NG   LVL+D  +PV+FKKV
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

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BLAST of Spg036546 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.1e-41
Identity = 80/200 (40.00%), Postives = 127/200 (63.50%), Query Frame = 0


Query: 7   LSFLFIVIA-STEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTIGSIRK 66
           L ++F+++A     R     A+ + V D+NG+ L  GVNY ILP      GGLT+ +++ 
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 67  GYDQCPVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGKCGKSTVWKVAK 126
             + CP +++ + +E S GLP  FSP + K   + VSTD+NI+F        +++W++A 
Sbjct: 64  --ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELAN 123

Query: 127 LDQSTGQYFVTIGGTKGNPGRETLDSWFKIEKLGNNYKFVHCPTVCKYCKVICKDVSIFY 186
            D++T Q+F++  G +GNPG++T+D+WFKI+K   +YK   CPTVC +CKVIC+DV +F 
Sbjct: 124 FDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFV 183

Query: 187 ANGRLTLVLNDAAYPVMFKK 206
            +G+  L L+D    VMFK+
Sbjct: 184 QDGKRRLALSDVPLKVMFKR 194

[Close]

BLAST of Spg036546 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 110.9 bits (276), Expect = 1.2e-24
Identity = 74/198 (37.37%), Postives = 101/198 (51.01%), Query Frame = 0


Query: 11  FIVIASTEVRLCKAGASPDAVLDVNGEKLRAGVNYSILPYFGRNAGGLTIGSIRKGYDQC 70
           ++V+A T V    A  +  AV+D++G  +    +Y +LP      GGLT+    +G   C
Sbjct: 13  YLVLALTAV---LASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG--RGGQPC 72

Query: 71  PVNIVPETYERSMGLPATFSPINPKKGVVRVSTDLNIQFQANGK-CGKSTVWKVAKLDQS 130
           P +IV E+ E   G+P  FS    K   V  S +LNI+       C +ST W+V + D  
Sbjct: 73  PYDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHE 132

Query: 131 TGQYFVTIGGTKGNPGRETLDSWFKIEKLGNN-YKFVHCPTVCKYCKVICKDVSIFYAN- 190
             QYFV  G      G+++L S+FKIEK G + YKFV CP  C      C DV IF    
Sbjct: 133 RKQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDEL 192

Query: 191 GRLTLVLNDAAYPVMFKK 206
           G   L L+D  + VMFKK
Sbjct: 193 GVRRLALSDKPFLVMFKK 204

The following BLAST results are available for this feature:

BLAST of Spg036546 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895396.1	7.7e-82	70.87	miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa his...	[more]
XP_008437082.1	3.8e-81	70.87	PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. mak...	[more]
XP_022137472.1	2.4e-75	66.50	kunitz trypsin inhibitor 2 [Momordica charantia]	[more]
XP_038893656.1	8.6e-73	64.93	miraculin-like [Benincasa hispida]	[more]
XP_008437071.1	2.1e-71	66.67	PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis mel...	[more]

BLAST of Spg036546 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P13087	1.8e-44	47.22	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
Q9LMU2	1.5e-40	40.00	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P32765	2.2e-31	39.05	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	1.7e-23	37.37	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
A0A072TH68	2.8e-18	28.29	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]

BLAST of Spg036546 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LT81	1.1e-81	70.39	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1	[more]
A0A5D3BJ02	1.9e-81	70.87	Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=...	[more]
A0A1S3AST3	1.9e-81	70.87	miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1	[more]
A0A6J1C8C4	1.2e-75	66.50	kunitz trypsin inhibitor 2 OS=Momordica charantia OX=3673 GN=LOC111008908 PE=3 S...	[more]
A0A5D3BJ25	1.0e-71	66.67	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00278...	[more]

BLAST of Spg036546 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	1.1e-41	40.00	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	1.2e-24	37.37	kunitz trypsin inhibitor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 70..90 score: 23.81 coord: 152..171 score: 25.88 coord: 30..59 score: 37.97
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 30..206 e-value: 5.6E-53 score: 192.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 31..205 e-value: 8.4E-49 score: 165.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 7..206
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 31..47
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 30..205 e-value: 2.27582E-53 score: 166.749
None	No IPR available	GENE3D	2.80.10.50		coord: 25..206 e-value: 1.5E-62 score: 212.4
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 7..206
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 27..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg036546.1	Spg036546.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity