Spg036318 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg036318
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionmajor pollen allergen Ole e 6-like
Locationscaffold5: 44954372 .. 44957114 (+)
RNA-Seq ExpressionSpg036318
SyntenySpg036318
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTCTTCAAACAGTCATCCACTCGCAAAGGAAAAGGTATAGGGAGGCGCAAAATCTGAAAAGATTTAAAAGCGTCGCCAAAAGGCAAGATAAAAGAAAATGGCCAAGAAGATGATTGCTGTGTTCTTGATGTGCATTGTGGTGGTGGCTGCATTGCAGAATAATGTCAATGCCGACGACAAAGAGACTAAATGGGAGGTTGAATATGAGGCTAAATTTGACGCCAAGTACAAGTCTTGCTACAAAAATTGCGAGAAGGAATGTCTCGAACATGGCAGTGACCAAAGCTTCTGTGAAGTTAAGTGTGACGAAGATTGTAACGAAAAGGAAGCTGCTGGTACTCTCTCTTCTTCTAACTTTTTTCAACGTCATTAGGTGTCCTGTTTGATATTTTCTACCAATTTTCCTACCGTGGTTTTCATATTTGTCAATGACATATTTGAATTCCTAGTCAAATTCTAAAAGCAAAAACGAAGTTTATGAAAACTATTTTTTTTAGTTTTCAAAATTTGCTTCCTTTTTTAAAAACAAGGAAAGGAAGGTATTAGAAAAAAAAAAATTAGGTGGAAGTAGGATTCATAAGCTTAATTTTCAAAAACCAAATGGTTATTAAACGAGACCTGAAATATTCTGACATTGTCACAAAATTGTCCATAGATCTCCGCATTTTCAAAGCACTTTTGAGAATGAAAGAAGGAATGGTTGTTGACCTTTATATCGATTTTTTTTTTTTTTAATTGTTGTCTCATATTAATTTAAATTATTGTATTTGTTTTACAGATAAGCTAAAGATCGACTTGGCCCATTGAACAATGGAGAACAAAAGAAAAACGAGCTCTTGAGAAGGATCTCTAGTAGTAATTGTATTTAAATCATGTTTGTGAGATCATTTCTCCTTTTTAATTTGGATCAGTTCTCTTTGGTGTCATATCGATATGTAAATCGACCATTTTCATGGTGTTATTTTTATTTATTCTAAAAGAAAAAAAATTATGATTTCCCCGACATTGTTGATTAACACTTACCTGGTTAAGGAGTGGTAAAAAAATTATTCGCCTTTTATGCAACAACAATAATATCCTAAATAATTCTTTGGTGTTATCAGCATGTATCCTCGAACAAAAGGTCAAAGTTTGAACCGTCACCTCCAAATGTTGTTATTTAAAATTTAAAAAAAAAAAACAAAAACAAACAAACAAACTCAAATAAAAAAGAGTAATTTCTCACATAAGTTACATGAAGGATAAGTCTATTTGCAATATGCACTTGGCAAATATATATATGTACAACAATCTTGTCTCCAATTCTTTTTCTAAATAATTATTATTTAATTTATTTTTTATTTCTATTATTAACTTTTTGGGGAAAAAAAATTAGTTTTCCGTGACTAAATCAATTTTCAAAACGGTTAATTTTATCGAATTGAATTTAAACTAAAATTAATTTGAAAATTTGCACATAAAACTCAACCAATAAAAAAAAAACTTAAGTTTGTAAGTAGAACTATCAAATTTAAAAATGATCTTAATCAAAATATCAAAACTTAATTAATAACTCTTGATATTTATTAGAATTTTGTATAAAAGAAAAGGAACATGGTTGAATATTGAAAAGATAAAAATGGATAGATATTATATGAAGGACGAATTTTAGGAGATGTTTTTTAATACGTGTTTGACGTAAAGCATATATCGAATACTTTTCGTTTATACCTACAATAGAGTAAATCACTTATAAATTAAAGTAATTCATGATTTCAGACAAGGCCATTTTGTAATTCCATAAAAAAAAAATACTAGACTTGTCCAAAGGCAAAAGTTTGAAAATTTTAAGACCGAACCCATAGATAATTAAAGACATAACTATAACACATACGAAATTTTCTTAGTCATTTTTATATTGTAATTGGATATTGATAGTTAAAATTCTTTAGATAGATTACATATTAAAATTCTGATGTGTTCATTTCTAGCTAGTTTTTTTTTTAGGTGGTGGATTTAAGTTGACTAAATATTGTTTATTCTTTTAGAAACATGTTTCAAAAATGGTTCCTAAAGTGTTTTATACACAATTATTATATACTTAAAAACTCTTTCTAAAAATTTTGCTAGTATTTCTCTTAAAAATAGTTTATTTCTTCATCTCATTTTTTAATAAGTTATTTTTAGTGGTTGTCAAACACTATACTTTTTTTTTCTAAATGATTTTCTTTTTCAAAATTAAACAATTGAAAAGTTAAACCAAATGCGGAATTGTCCAATTACTTTTTGTGTTTTTTTTTTTTTTGATCCGTTTATGTTATGATGCTTAAGAACAATTCCCCTCGTAAGAAATAGAATCAATTGTTTTTTTGTTTCTTTTAACTAAAATTACATTAGTTACCTTTCACAATTTTTCAAATGAAGAGTACTTTTTATTGATGGAAAATTGATATATATATATATATATATATATATATATATATATATATTTAGACTTACCAGTTTCCAACCAAAGGGCTAAAATTCCCTTCGCTTACTCATGACCAAAAGTCAATAGATTATAAGTTTTAAACTTAAAATACTCATTGGAAGGGAAAGCCATGATTGAAAATTTGGAAGGGAAAGCCATGATCGAAGGGTTGAAGGCTTACCTATCTCATCCCAAGATAAAGGGGAGCAGAGTCAAAGTCCCCCTCGAGGTTGAAAGCGACTCTTTGGAGTTCGTGGAGGCGATTTTCGGGCATTCAGCTGATATCTCCTAA

mRNA sequence

ATGGCCAAGAAGATGATTGCTGTGTTCTTGATGTGCATTGTGGTGGTGGCTGCATTGCAGAATAATGTCAATGCCGACGACAAAGAGACTAAATGGGAGGTTGAATATGAGGCTAAATTTGACGCCAAGTACAAGTCTTGCTACAAAAATTGCGAGAAGGAATGTCTCGAACATGGCAGTGACCAAAGCTTCTGTGAAGTTAAGTGTGACGAAGATTGTAACGAAAAGGAAGCTGCTGGGAAAGCCATGATTGAAAATTTGGAAGGGAAAGCCATGATCGAAGGGTTGAAGGCTTACCTATCTCATCCCAAGATAAAGGGGAGCAGAGTCAAAGTCCCCCTCGAGGTTGAAAGCGACTCTTTGGAGTTCGTGGAGGCGATTTTCGGGCATTCAGCTGATATCTCCTAA

Coding sequence (CDS)

ATGGCCAAGAAGATGATTGCTGTGTTCTTGATGTGCATTGTGGTGGTGGCTGCATTGCAGAATAATGTCAATGCCGACGACAAAGAGACTAAATGGGAGGTTGAATATGAGGCTAAATTTGACGCCAAGTACAAGTCTTGCTACAAAAATTGCGAGAAGGAATGTCTCGAACATGGCAGTGACCAAAGCTTCTGTGAAGTTAAGTGTGACGAAGATTGTAACGAAAAGGAAGCTGCTGGGAAAGCCATGATTGAAAATTTGGAAGGGAAAGCCATGATCGAAGGGTTGAAGGCTTACCTATCTCATCCCAAGATAAAGGGGAGCAGAGTCAAAGTCCCCCTCGAGGTTGAAAGCGACTCTTTGGAGTTCGTGGAGGCGATTTTCGGGCATTCAGCTGATATCTCCTAA

Protein sequence

MAKKMIAVFLMCIVVVAALQNNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHGSDQSFCEVKCDEDCNEKEAAGKAMIENLEGKAMIEGLKAYLSHPKIKGSRVKVPLEVESDSLEFVEAIFGHSADIS
Homology
BLAST of Spg036318 vs. NCBI nr
Match: XP_008456958.1 (PREDICTED: major pollen allergen Ole e 6-like [Cucumis melo] >KAA0066995.1 major pollen allergen Ole e 6-like [Cucumis melo var. makuwa] >TYJ96804.1 major pollen allergen Ole e 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 117.1 bits (292), Expect = 1.2e-22
Identity = 63/86 (73.26%), Postives = 70/86 (81.40%), Query Frame = 0

Query: 1  MAKKMIAVFLMCIVVVAALQ-NNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHG 60
          MAKKMIA+FLMCIVVVAALQ  + N +D+  K    YEAKFDAKYKSC+K CEKECLE+G
Sbjct: 1  MAKKMIALFLMCIVVVAALQFASANKEDEVAK----YEAKFDAKYKSCFKTCEKECLENG 60

Query: 61 SDQSFCEVKCDEDCNEKEAAGKAMIE 86
          S QS+CEVKCDEDCNEKE A K  IE
Sbjct: 61 SGQSYCEVKCDEDCNEKEVADKLHIE 82

BLAST of Spg036318 vs. NCBI nr
Match: XP_038891517.1 (major pollen allergen Ole e 6-like [Benincasa hispida])

HSP 1 Score: 114.0 bits (284), Expect = 1.0e-21
Identity = 61/85 (71.76%), Postives = 68/85 (80.00%), Query Frame = 0

Query: 1  MAKKMIAVFLMCIVVVAALQNNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHGS 60
          MAKKM+AVFLMCIVVVAALQ     +D+ TK    YEAKFDAKYKSCY+ CEKECL +GS
Sbjct: 1  MAKKMMAVFLMCIVVVAALQFATANEDEVTK----YEAKFDAKYKSCYETCEKECLANGS 60

Query: 61 DQSFCEVKCDEDCNEKEAAGKAMIE 86
           QSFCEVKCDEDC+EKE A K  I+
Sbjct: 61 GQSFCEVKCDEDCDEKEVADKLHIK 81

BLAST of Spg036318 vs. NCBI nr
Match: XP_004146471.1 (major pollen allergen Ole e 6 [Cucumis sativus] >KGN50739.1 hypothetical protein Csa_005859 [Cucumis sativus])

HSP 1 Score: 110.9 bits (276), Expect = 8.5e-21
Identity = 60/86 (69.77%), Postives = 68/86 (79.07%), Query Frame = 0

Query: 1  MAKKMIAVFLMCIVVVAALQ-NNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHG 60
          MA KMIA+FLMCIVVVAALQ  N N +D       +YEAKFDAKYKSC++ CEKECL +G
Sbjct: 1  MANKMIALFLMCIVVVAALQFANANKED-----VAKYEAKFDAKYKSCFETCEKECLRYG 60

Query: 61 SDQSFCEVKCDEDCNEKEAAGKAMIE 86
          S QS+CEVKCDEDC+EKEAA K  IE
Sbjct: 61 SGQSYCEVKCDEDCDEKEAADKLHIE 81

BLAST of Spg036318 vs. NCBI nr
Match: XP_038875643.1 (major pollen allergen Ole e 6-like [Benincasa hispida] >XP_038906512.1 major pollen allergen Ole e 6-like [Benincasa hispida])

HSP 1 Score: 109.4 bits (272), Expect = 2.5e-20
Identity = 58/85 (68.24%), Postives = 67/85 (78.82%), Query Frame = 0

Query: 1  MAKKMIAVFLMCIVVVAALQNNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHGS 60
          MAKKMIAVF MCIVV+AALQ     ++ +     +YEAKFDAKYKSCY+ CEKECLE+GS
Sbjct: 1  MAKKMIAVFFMCIVVMAALQFATANEEVD-----KYEAKFDAKYKSCYETCEKECLENGS 60

Query: 61 DQSFCEVKCDEDCNEKEAAGKAMIE 86
           Q+FCEVKCDEDC+EKE A K  IE
Sbjct: 61 GQTFCEVKCDEDCDEKEKADKLHIE 80

BLAST of Spg036318 vs. NCBI nr
Match: XP_038906682.1 (major pollen allergen Ole e 6-like [Benincasa hispida])

HSP 1 Score: 109.4 bits (272), Expect = 2.5e-20
Identity = 60/85 (70.59%), Postives = 68/85 (80.00%), Query Frame = 0

Query: 1  MAKKMIAVFLMCIVVVAALQNNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHGS 60
          MAKKMIAVFLMCIVV+AALQ    A+++  K    YEAKFDAKYKSCY+ CEKECLE G+
Sbjct: 1  MAKKMIAVFLMCIVVMAALQ-FTTANEEVAK----YEAKFDAKYKSCYETCEKECLEKGN 60

Query: 61 DQSFCEVKCDEDCNEKEAAGKAMIE 86
           QSFCEVKCDEDC+EKE A K  I+
Sbjct: 61 GQSFCEVKCDEDCDEKETADKLHIK 80

BLAST of Spg036318 vs. ExPASy Swiss-Prot
Match: O24172 (Major pollen allergen Ole e 6 OS=Olea europaea OX=4146 GN=OLE6 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 4.0e-05
Identity = 22/50 (44.00%), Postives = 33/50 (66.00%), Query Frame = 0

Query: 41 DAKYKSCYKNCEKECLEHGSDQSFCEVKCDEDCNEKEAAGKAMIENLEGK 91
          +A++K CY  C KEC + G+  +FCE+KCD DC+ K+   K  +EN + K
Sbjct: 2  EAQFKECYDTCHKECSDKGNGFTFCEMKCDTDCSVKDV--KEKLENYKPK 49

BLAST of Spg036318 vs. ExPASy TrEMBL
Match: A0A5D3BCS2 (Major pollen allergen Ole e 6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold233G00230 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 5.8e-23
Identity = 63/86 (73.26%), Postives = 70/86 (81.40%), Query Frame = 0

Query: 1  MAKKMIAVFLMCIVVVAALQ-NNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHG 60
          MAKKMIA+FLMCIVVVAALQ  + N +D+  K    YEAKFDAKYKSC+K CEKECLE+G
Sbjct: 1  MAKKMIALFLMCIVVVAALQFASANKEDEVAK----YEAKFDAKYKSCFKTCEKECLENG 60

Query: 61 SDQSFCEVKCDEDCNEKEAAGKAMIE 86
          S QS+CEVKCDEDCNEKE A K  IE
Sbjct: 61 SGQSYCEVKCDEDCNEKEVADKLHIE 82

BLAST of Spg036318 vs. ExPASy TrEMBL
Match: A0A1S3C530 (major pollen allergen Ole e 6-like OS=Cucumis melo OX=3656 GN=LOC103496750 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 5.8e-23
Identity = 63/86 (73.26%), Postives = 70/86 (81.40%), Query Frame = 0

Query: 1  MAKKMIAVFLMCIVVVAALQ-NNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHG 60
          MAKKMIA+FLMCIVVVAALQ  + N +D+  K    YEAKFDAKYKSC+K CEKECLE+G
Sbjct: 1  MAKKMIALFLMCIVVVAALQFASANKEDEVAK----YEAKFDAKYKSCFKTCEKECLENG 60

Query: 61 SDQSFCEVKCDEDCNEKEAAGKAMIE 86
          S QS+CEVKCDEDCNEKE A K  IE
Sbjct: 61 SGQSYCEVKCDEDCNEKEVADKLHIE 82

BLAST of Spg036318 vs. ExPASy TrEMBL
Match: A0A0A0KPA7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G222980 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.1e-21
Identity = 60/86 (69.77%), Postives = 68/86 (79.07%), Query Frame = 0

Query: 1  MAKKMIAVFLMCIVVVAALQ-NNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHG 60
          MA KMIA+FLMCIVVVAALQ  N N +D       +YEAKFDAKYKSC++ CEKECL +G
Sbjct: 1  MANKMIALFLMCIVVVAALQFANANKED-----VAKYEAKFDAKYKSCFETCEKECLRYG 60

Query: 61 SDQSFCEVKCDEDCNEKEAAGKAMIE 86
          S QS+CEVKCDEDC+EKEAA K  IE
Sbjct: 61 SGQSYCEVKCDEDCDEKEAADKLHIE 81

BLAST of Spg036318 vs. ExPASy TrEMBL
Match: A0A5A7TDR1 (Carotenoid cleavage dioxygenase 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002630 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 6.0e-20
Identity = 56/81 (69.14%), Postives = 67/81 (82.72%), Query Frame = 0

Query: 1  MAKKMIAVFLMCIVVVAALQNNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHGS 60
          MAKKM+AVFLMCIV+VA LQ  +  ++ E     +YEAKFDAKYKSCY+ CEKECL+ G+
Sbjct: 1  MAKKMMAVFLMCIVLVATLQFAIAKEEDEV---TKYEAKFDAKYKSCYETCEKECLQKGN 60

Query: 61 D-QSFCEVKCDEDCNEKEAAG 81
          + QSFCEVKCDEDC+EKEAAG
Sbjct: 61 NGQSFCEVKCDEDCSEKEAAG 78

BLAST of Spg036318 vs. ExPASy TrEMBL
Match: A0A6J1EQ21 (major pollen allergen Ole e 6-like OS=Cucurbita moschata OX=3662 GN=LOC111436354 PE=4 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 1.3e-19
Identity = 58/85 (68.24%), Postives = 68/85 (80.00%), Query Frame = 0

Query: 1  MAKKMIAVFLMCIVVVAALQNNVNADDKETKWEVEYEAKFDAKYKSCYKNCEKECLEHGS 60
          MAKKMIAV L+CIVVVAALQ  V++  +  K E +YEAKF+AKY+ CY+ CEKECLE G+
Sbjct: 1  MAKKMIAVLLVCIVVVAALQ--VSSATESAK-EAKYEAKFEAKYRLCYEKCEKECLEKGN 60

Query: 61 DQSFCEVKCDEDCNEKEAAGKAMIE 86
           QSFCEVKCDEDC EKEAA K  I+
Sbjct: 61 GQSFCEVKCDEDCGEKEAADKLHIK 82

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008456958.11.2e-2273.26PREDICTED: major pollen allergen Ole e 6-like [Cucumis melo] >KAA0066995.1 major... [more]
XP_038891517.11.0e-2171.76major pollen allergen Ole e 6-like [Benincasa hispida][more]
XP_004146471.18.5e-2169.77major pollen allergen Ole e 6 [Cucumis sativus] >KGN50739.1 hypothetical protein... [more]
XP_038875643.12.5e-2068.24major pollen allergen Ole e 6-like [Benincasa hispida] >XP_038906512.1 major pol... [more]
XP_038906682.12.5e-2070.59major pollen allergen Ole e 6-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O241724.0e-0544.00Major pollen allergen Ole e 6 OS=Olea europaea OX=4146 GN=OLE6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BCS25.8e-2373.26Major pollen allergen Ole e 6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C5305.8e-2373.26major pollen allergen Ole e 6-like OS=Cucumis melo OX=3656 GN=LOC103496750 PE=4 ... [more]
A0A0A0KPA74.1e-2169.77Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G222980 PE=4 SV=1[more]
A0A5A7TDR16.0e-2069.14Carotenoid cleavage dioxygenase 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1EQ211.3e-1968.24major pollen allergen Ole e 6-like OS=Cucurbita moschata OX=3662 GN=LOC111436354... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036466Pollen allergen ole e 6 superfamilyGENE3D1.10.287.720Pollen allergen ole e 6coord: 41..87
e-value: 2.8E-13
score: 51.2
IPR036466Pollen allergen ole e 6 superfamilySUPERFAMILY111388Pollen allergen ole e 6coord: 41..88
IPR015333Pollen allergen ole e 6PFAMPF09253Ole_e_6coord: 47..81
e-value: 8.6E-11
score: 41.6
IPR015333Pollen allergen ole e 6PANTHERPTHR35632MAJOR POLLEN ALLERGEN OLE E 6-LIKEcoord: 1..85
NoneNo IPR availablePANTHERPTHR35632:SF5SUBFAMILY NOT NAMEDcoord: 1..85

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg036318.1Spg036318.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
biological_process GO:0009733 response to auxin
biological_process GO:0010223 secondary shoot formation
biological_process GO:1901601 strigolactone biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen