Spg034393 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg034393 vs. NCBI nr
Match: XP_023532565.1 (molybdenum cofactor sulfurase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1104.0 bits (2854), Expect = 0.0e+00
Identity = 558/625 (89.28%), Postives = 587/625 (93.92%), Query Frame = 0

Query: 1   MATACGGGRGGGSSCCPTPLLNFPSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTN 60
           +A ACGGG GGGSSCCPTPLLNFPSSTSTT+TT   PRTSAASRRDF AKA+AGVFPNTN
Sbjct: 9   IAAACGGGSGGGSSCCPTPLLNFPSSTSTTSTT---PRTSAASRRDFTAKATAGVFPNTN 68

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           FTNPE+LP PPQAL+LFLAAFPQY++TQ+IDQIRNKQYYHLN +NHTCLDYIGIGLFSYH
Sbjct: 69  FTNPESLPAPPQALALFLAAFPQYNQTQKIDQIRNKQYYHLNFTNHTCLDYIGIGLFSYH 128

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDY 180
           QFQKH NPFPSSNLNFPFFGVSY++GN KSRLLEN LDSDLESA+KRRIFRFLNVS+SDY
Sbjct: 129 QFQKHHNPFPSSNLNFPFFGVSYRTGNFKSRLLENGLDSDLESAIKRRIFRFLNVSDSDY 188

Query: 181 AMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 240
           +MVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMV+TSQNRGALTMSAEFSWP
Sbjct: 189 SMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVNTSQNRGALTMSAEFSWP 248

Query: 241 RLRINSRKLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDAC 300
           RLRINSRKLKEMIVSKNKKKK KKGLFVFPLHSRITGARYPYLWMS+AQENRWHVLVDAC
Sbjct: 249 RLRINSRKLKEMIVSKNKKKKTKKGLFVFPLHSRITGARYPYLWMSIAQENRWHVLVDAC 308

Query: 301 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIV 360
           ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISIL+ NSCSSNVGIV
Sbjct: 309 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILDTNSCSSNVGIV 368

Query: 361 NLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKT----RTQFP 420
           NLVPADKL QLAEDSSGTDTELEI H+QFMSQMVPSTSSFSGPIS+ SMKT     TQFP
Sbjct: 369 NLVPADKLFQLAEDSSGTDTELEIHHQQFMSQMVPSTSSFSGPISYESMKTAQTSTTQFP 428

Query: 421 EMEEEGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKH 480
           E EE+ NL S VSEI       ETKC+GLDQ+DSLGLILISTRARCLINWLVSSLLKLKH
Sbjct: 429 ETEEDTNLRSNVSEI-------ETKCKGLDQMDSLGLILISTRARCLINWLVSSLLKLKH 488

Query: 481 PNSEGVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNI 540
           PN+EGV LVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFL NI
Sbjct: 489 PNTEGVSLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLQNI 548

Query: 541 WFSDKYAEEKGKVLERKESGKDEKLKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQF 600
           WF +KYAEEKG+VLERKE GKDEK+KK KANLGISVVTAALGFLTNF+DVYKLW FVAQF
Sbjct: 549 WFPEKYAEEKGRVLERKEVGKDEKMKKVKANLGISVVTAALGFLTNFDDVYKLWCFVAQF 608

Query: 601 LDADFVEKERWRYTALNQRTIIEVL 622
           LDADFVEKERWRYTALNQRT++EVL
Sbjct: 609 LDADFVEKERWRYTALNQRTVLEVL 623

BLAST of Spg034393 vs. NCBI nr
Match: KAG6605688.1 (Molybdenum cofactor sulfurase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035597.1 Molybdenum cofactor sulfurase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 557/625 (89.12%), Postives = 587/625 (93.92%), Query Frame = 0

Query: 1   MATACGGGRGGGSSCCPTPLLNFPSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTN 60
           +A ACGGG GGGSSCCPTPLLNFPSSTSTT+TT   PRTSAASRRDF AKA+AGVFPNTN
Sbjct: 9   IAAACGGGSGGGSSCCPTPLLNFPSSTSTTSTT---PRTSAASRRDFTAKATAGVFPNTN 68

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           FTNPE+LP PPQAL+LFLAAFPQY++TQ+IDQIRNKQYYHLN +NHTCLDYIGIGLFSYH
Sbjct: 69  FTNPESLPAPPQALALFLAAFPQYNQTQKIDQIRNKQYYHLNFTNHTCLDYIGIGLFSYH 128

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDY 180
           QFQKH NPFPSSNLNFPFFGVSY++GN KSRLLEN LDSDLESA+KRRIFRFLNVS+SDY
Sbjct: 129 QFQKHHNPFPSSNLNFPFFGVSYRTGNFKSRLLENGLDSDLESAIKRRIFRFLNVSDSDY 188

Query: 181 AMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 240
           +MVFTANRTSAFKLLAESYPF TSNKLLTVYDYESEAVEAMV+TSQNRGALTMSAEFSWP
Sbjct: 189 SMVFTANRTSAFKLLAESYPFHTSNKLLTVYDYESEAVEAMVNTSQNRGALTMSAEFSWP 248

Query: 241 RLRINSRKLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDAC 300
           RLRINSRKLKEMIVSKNKKKK KKGLFVFPLHSRITGARYPYLWMS+AQENRWHVLVDAC
Sbjct: 249 RLRINSRKLKEMIVSKNKKKKTKKGLFVFPLHSRITGARYPYLWMSIAQENRWHVLVDAC 308

Query: 301 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIV 360
           ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISIL+ NSCSSNVGIV
Sbjct: 309 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILDTNSCSSNVGIV 368

Query: 361 NLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKT----RTQFP 420
           NLVPADKL QLAEDSSGTDTELEI H+QFMSQMVPSTSSFSGPIS+ SMKT     TQFP
Sbjct: 369 NLVPADKLFQLAEDSSGTDTELEIHHQQFMSQMVPSTSSFSGPISYESMKTAQTSTTQFP 428

Query: 421 EMEEEGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKH 480
           + EE+ NLGS VSEI       ETKC+GLDQ+DSLGLILISTRARCLINWLVSSLLKLKH
Sbjct: 429 KTEEDTNLGSNVSEI-------ETKCKGLDQMDSLGLILISTRARCLINWLVSSLLKLKH 488

Query: 481 PNSEGVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNI 540
           PN+EGV LVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFL NI
Sbjct: 489 PNTEGVSLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLQNI 548

Query: 541 WFSDKYAEEKGKVLERKESGKDEKLKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQF 600
           WF +KYAEEKG+VLERKE GKDEK+KK KANLGISVVTAALGFLTNF+DVYKLW FVAQF
Sbjct: 549 WFPEKYAEEKGRVLERKEVGKDEKMKKVKANLGISVVTAALGFLTNFDDVYKLWCFVAQF 608

Query: 601 LDADFVEKERWRYTALNQRTIIEVL 622
           LDADFVEKERWRYTALNQRT++EVL
Sbjct: 609 LDADFVEKERWRYTALNQRTVLEVL 623

BLAST of Spg034393 vs. NCBI nr
Match: XP_022957621.1 (molybdenum cofactor sulfurase [Cucurbita moschata])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 557/625 (89.12%), Postives = 587/625 (93.92%), Query Frame = 0

Query: 1   MATACGGGRGGGSSCCPTPLLNFPSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTN 60
           +A ACGGG GGGSSCCPTPLLNFPSSTSTT+TT   PRTSAASRRDF AKA+AGVFPNTN
Sbjct: 9   IAAACGGGSGGGSSCCPTPLLNFPSSTSTTSTT---PRTSAASRRDFTAKATAGVFPNTN 68

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           FTNPE+LP PPQAL+LFLAAFPQY++TQ+IDQIRNKQYYHLN +NHTCLDYIGIGLFSYH
Sbjct: 69  FTNPESLPAPPQALALFLAAFPQYNQTQKIDQIRNKQYYHLNFTNHTCLDYIGIGLFSYH 128

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDY 180
           QFQKH NPFPSSNLNFPFFGVSY++GN KSRLLEN LDSDLESA+KRRIFRFLNVS+SDY
Sbjct: 129 QFQKHHNPFPSSNLNFPFFGVSYRTGNFKSRLLENGLDSDLESAIKRRIFRFLNVSDSDY 188

Query: 181 AMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 240
           +MVFTANRTSAFKLLAESYPF TS+KLLTVYDYESEAVEAMV+TSQNRGALTMSAEFSWP
Sbjct: 189 SMVFTANRTSAFKLLAESYPFHTSSKLLTVYDYESEAVEAMVNTSQNRGALTMSAEFSWP 248

Query: 241 RLRINSRKLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDAC 300
           RLRINSRKLKEMIVSKNKKKK KKGLFVFPLHSRITGARYPYLWMS+AQENRWHVLVDAC
Sbjct: 249 RLRINSRKLKEMIVSKNKKKKTKKGLFVFPLHSRITGARYPYLWMSIAQENRWHVLVDAC 308

Query: 301 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIV 360
           ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISIL+ NSCSSNVGIV
Sbjct: 309 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILDTNSCSSNVGIV 368

Query: 361 NLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKT----RTQFP 420
           NLVPADKL QLAEDSSGTDTELEI H+QFMSQMVPSTSSFSGPIS+ SMKT     TQFP
Sbjct: 369 NLVPADKLFQLAEDSSGTDTELEIHHQQFMSQMVPSTSSFSGPISYESMKTAQTPTTQFP 428

Query: 421 EMEEEGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKH 480
           E EE+ NLGS VSEI       ETKC+GLDQ+DSLGLILISTRARCLINWLVSSLLKLKH
Sbjct: 429 ETEEDPNLGSNVSEI-------ETKCKGLDQMDSLGLILISTRARCLINWLVSSLLKLKH 488

Query: 481 PNSEGVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNI 540
           PN+EGV LVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFL NI
Sbjct: 489 PNTEGVSLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLQNI 548

Query: 541 WFSDKYAEEKGKVLERKESGKDEKLKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQF 600
           WF +KYAEEKG+VLERKE GKDEK+KK KANLGISVVTAALGFLTNF+DVYKLW FVAQF
Sbjct: 549 WFPEKYAEEKGRVLERKEVGKDEKMKKVKANLGISVVTAALGFLTNFDDVYKLWCFVAQF 608

Query: 601 LDADFVEKERWRYTALNQRTIIEVL 622
           LDADFVEKERWRYTALNQRT++EVL
Sbjct: 609 LDADFVEKERWRYTALNQRTVLEVL 623

BLAST of Spg034393 vs. NCBI nr
Match: XP_022995307.1 (molybdenum cofactor sulfurase [Cucurbita maxima])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 557/625 (89.12%), Postives = 585/625 (93.60%), Query Frame = 0

Query: 1   MATACGGGRGGGSSCCPTPLLNFPSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTN 60
           +A ACGGG GGGSSCCPTPLLNFPSSTSTT+TT   PRTSAASRRDF AKA AGVFPNTN
Sbjct: 9   IAAACGGGSGGGSSCCPTPLLNFPSSTSTTSTT---PRTSAASRRDFTAKAIAGVFPNTN 68

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           FTNPE+LP PPQALSLFLAAFPQY++TQ+IDQIRNKQYYHLN +NHTCLDYIGIGLFSYH
Sbjct: 69  FTNPESLPGPPQALSLFLAAFPQYNQTQKIDQIRNKQYYHLNFTNHTCLDYIGIGLFSYH 128

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDY 180
           QFQKH NPFPSSNLNFPFFGVSY++GN KSRLLEN LDSDLESA+KRRIFRFLNVS+SDY
Sbjct: 129 QFQKHHNPFPSSNLNFPFFGVSYRTGNFKSRLLENGLDSDLESAIKRRIFRFLNVSDSDY 188

Query: 181 AMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 240
           +MVFTANRTSAFKLLAESYPF TSNKLLTVYDYESEAVEAMV+TSQNRGALTMSAEFSWP
Sbjct: 189 SMVFTANRTSAFKLLAESYPFHTSNKLLTVYDYESEAVEAMVNTSQNRGALTMSAEFSWP 248

Query: 241 RLRINSRKLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDAC 300
           RLRINSRKLKEMIVSKNKKKK  KGLFVFPLHSRITGARYPYLWMS+AQENRWHVLVDAC
Sbjct: 249 RLRINSRKLKEMIVSKNKKKKTNKGLFVFPLHSRITGARYPYLWMSIAQENRWHVLVDAC 308

Query: 301 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIV 360
           ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKK+VISIL+ NSCSSNVGIV
Sbjct: 309 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKTVISILDTNSCSSNVGIV 368

Query: 361 NLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKT----RTQFP 420
           NLVPADKL QLAEDSSGTDTELEI H+QFMSQMVPSTSSFSGPIS+ SMKT     TQFP
Sbjct: 369 NLVPADKLFQLAEDSSGTDTELEIHHQQFMSQMVPSTSSFSGPISYESMKTAQTSTTQFP 428

Query: 421 EMEEEGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKH 480
           E EE+ NLGS VSEI       ETKC+GLDQ+DSLGLILISTRARCLINWLVSSLLKLKH
Sbjct: 429 ETEEDPNLGSNVSEI-------ETKCKGLDQMDSLGLILISTRARCLINWLVSSLLKLKH 488

Query: 481 PNSEGVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNI 540
           PN+EGV LVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFL NI
Sbjct: 489 PNTEGVSLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLQNI 548

Query: 541 WFSDKYAEEKGKVLERKESGKDEKLKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQF 600
           WF +KYAEEKG+VLERKE GKDEK+KK KANLGISVVTAALGFLTNF+DVYKLW FVAQF
Sbjct: 549 WFPEKYAEEKGRVLERKEVGKDEKMKKVKANLGISVVTAALGFLTNFDDVYKLWCFVAQF 608

Query: 601 LDADFVEKERWRYTALNQRTIIEVL 622
           LDADFVEKERWRYTALNQRT++EVL
Sbjct: 609 LDADFVEKERWRYTALNQRTVLEVL 623

BLAST of Spg034393 vs. NCBI nr
Match: XP_038906632.1 (molybdenum cofactor sulfurase [Benincasa hispida])

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 551/622 (88.59%), Postives = 579/622 (93.09%), Query Frame = 0

Query: 1   MATACGGGRGGGSSCCPTPLLNFPSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTN 60
           ++ ACGGG GGGSSCCPTPLL+FPSS ++TT T   PRTSAASRRDFAAKASAGVFPNT 
Sbjct: 9   ISAACGGGSGGGSSCCPTPLLHFPSSQNSTTAT---PRTSAASRRDFAAKASAGVFPNTT 68

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           FTNPE LP PPQALSLFLAAFPQYH+TQEID+IRNKQYYHLNLSNH CLDYIGIGLFSYH
Sbjct: 69  FTNPECLPSPPQALSLFLAAFPQYHQTQEIDEIRNKQYYHLNLSNHICLDYIGIGLFSYH 128

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDY 180
           QFQKH NPFPSSNLNFPFFGVSY++GNLKSRLLEN LDSDLESA+KRRIFRFLNVS+SDY
Sbjct: 129 QFQKHSNPFPSSNLNFPFFGVSYRTGNLKSRLLENGLDSDLESAIKRRIFRFLNVSDSDY 188

Query: 181 AMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 240
           AM+FTANRTSAFKLLAESYPFQTSNK+LTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP
Sbjct: 189 AMIFTANRTSAFKLLAESYPFQTSNKVLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 248

Query: 241 RLRINSRKLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDAC 300
           RLRINSRKLKEMIVSKNKKKK+KKGLFVFPLHSRITGARYPYLWMS+AQENRWHVLVDAC
Sbjct: 249 RLRINSRKLKEMIVSKNKKKKSKKGLFVFPLHSRITGARYPYLWMSIAQENRWHVLVDAC 308

Query: 301 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIV 360
           ALGPKDMDCFGLSL RPDFLV SFYKVFGENPSGFGCLLVKKSVISILE NS SSN+GIV
Sbjct: 309 ALGPKDMDCFGLSLLRPDFLVSSFYKVFGENPSGFGCLLVKKSVISILESNSSSSNIGIV 368

Query: 361 NLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKTRTQFPEMEE 420
           NLVPADKLLQL EDSSGTDT+LE Q +     MVPSTSSFSGPIS+ S+KT T F E+E+
Sbjct: 369 NLVPADKLLQLNEDSSGTDTDLEFQQQH---HMVPSTSSFSGPISYQSLKTTTHFREIEK 428

Query: 421 EGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKHPNSE 480
           E  LGS V EI+ VS Q ETKC+GLDQVDSLGL+LISTRARCLINWLVSSLLKLKHPNS+
Sbjct: 429 EQKLGSNVPEIQTVSNQYETKCKGLDQVDSLGLVLISTRARCLINWLVSSLLKLKHPNSQ 488

Query: 481 GVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSD 540
           GVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADR+NISLSYGFLHNIWF D
Sbjct: 489 GVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRNNISLSYGFLHNIWFPD 548

Query: 541 KYAEEKGKVLERKESGKDEK-LKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQFLDA 600
           KY EEKGKVLERKE GKDEK +KK K NLGISVVTAALGFLTNFEDVYKLW FVAQFLDA
Sbjct: 549 KYGEEKGKVLERKEFGKDEKIMKKGKGNLGISVVTAALGFLTNFEDVYKLWGFVAQFLDA 608

Query: 601 DFVEKERWRYTALNQRTIIEVL 622
           DFVEKERWRYTALNQRTIIEVL
Sbjct: 609 DFVEKERWRYTALNQRTIIEVL 624

BLAST of Spg034393 vs. ExPASy Swiss-Prot
Match: Q9C5X8 (Molybdenum cofactor sulfurase OS=Arabidopsis thaliana OX=3702 GN=ABA3 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 3.4e-19
Identity = 135/564 (23.94%), Postives = 220/564 (39.01%), Query Frame = 0

Query: 74  LSLFLAAFPQYH----ETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYHQFQKHPNPF 133
           +  FL  F  Y+      + I +IR+ ++  L+      LD+ G  L+S  Q +     F
Sbjct: 1   MEAFLKEFGDYYGYPDGPKNIQEIRDTEFKRLD-KGVVYLDHAGSTLYSELQMEYIFKDF 60

Query: 134 PSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDYAMVFTANRT 193
            S+      FG  +   ++ S        SDL +  + ++  + N S  DY+ +FT+  T
Sbjct: 61  TSN-----VFGNPHSQSDISS------ATSDLIADARHQVLEYFNASPEDYSCLFTSGAT 120

Query: 194 SAFKLLAESYPF-QTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAE----------FS 253
           +A KL+ E++P+ Q SN L T+ ++ S       + +Q   A  +  E           S
Sbjct: 121 AALKLVGETFPWTQDSNFLYTMENHNSVLGIREYALAQGASACAVDIEEAANQPGQLTNS 180

Query: 254 WPRLRINSRKLKEMIVSKNKKKKAK---KGLFVFPLHSRITGARYPYLWMSVAQEN---- 313
            P +++  R ++    SK +K++++     LF FP     +G R+    + + +EN    
Sbjct: 181 GPSIKVKHRAVQMRNTSKLQKEESRGNAYNLFAFPSECNFSGLRFNLDLVKLMKENTETV 240

Query: 314 ----------RWHVLVDA---CALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCL 373
                     RW VL+DA   CA  P D     LS +  DF+V SFYK+FG  P+G G L
Sbjct: 241 LQGSPFSKSKRWMVLIDAAKGCATLPPD-----LSEYPADFVVLSFYKLFG-YPTGLGAL 300

Query: 374 LVKKSVISILEGNSCSSNVGIVNLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTS 433
           LV+     +L+    S      ++   D                                
Sbjct: 301 LVRNDAAKLLKKTYFSGGTVAASIADID-------------------------------- 360

Query: 434 SFSGPISHPSMKTRTQFPEMEEEGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILIST 493
                     +K R +  E  E+G+  +    I A+         G   + SL    I  
Sbjct: 361 ---------FVKRRERVEEFFEDGS--ASFLSIAAIR-------HGFKLLKSLTPSAIWM 420

Query: 494 RARCLINWLVSSLLKLKHPNSEGVCLVKIYGPK----VKFDRGPALAFNVFDWKGEKVEP 553
               L  ++   L  L+H N   VC+  +YG +         GP + FN+    G     
Sbjct: 421 HTTSLSIYVKKKLQALRHGNGAAVCV--LYGSENLELSSHKSGPTVTFNLKRPDGSWFGY 480

Query: 554 VLVQKLADRSNISLSYGFLHNIWFSDKYAE-EKGKVLERKESG----KDEKLKKAKANLG 594
           + V+KLA  S I L  G   N     KY E    ++    E+G     D  +   K    
Sbjct: 481 LEVEKLASLSGIQLRTGCFCNPGACAKYLELSHSELRSNVEAGHICWDDNDVINGKPT-- 491

BLAST of Spg034393 vs. ExPASy Swiss-Prot
Match: Q8LGM7 (Molybdenum cofactor sulfurase OS=Solanum lycopersicum OX=4081 GN=FLACCA PE=2 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.4e-17
Identity = 133/565 (23.54%), Postives = 227/565 (40.18%), Query Frame = 0

Query: 77  FLAAFPQYH----ETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYHQFQKHPNPFPSS 136
           FL  F  Y+      + ID+IR  ++  LN  +   LD+ G  L+S  Q +         
Sbjct: 10  FLKEFGSYYGYANSPKNIDEIRATEFKRLN--DTVYLDHAGATLYSESQMEA-----VFK 69

Query: 137 NLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDYAMVFTANRTSAF 196
           +LN   +      GN  S+   +    D+    ++++  F N S  +Y+ +FT+  T+A 
Sbjct: 70  DLNSTLY------GNPHSQSTCSLATEDIVGKARQQVLSFFNASPREYSCIFTSGATAAL 129

Query: 197 KLLAESYPFQTSNKLLTVYDYES-EAVEAMVSTSQNRGALTMSAEFSWPRL------RIN 256
           KL+ E++P+ +++  +  Y  E+  +V  +   + ++GA   + +     +      + N
Sbjct: 130 KLVGETFPWSSNSSFM--YSMENHNSVLGIREYALSKGAAAFAVDIEDTHVGESESPQSN 189

Query: 257 SRKLKEMIVSKNKKKKAKKG-------LFVFPLHSRITGARYPYLWMSVAQENR------ 316
            +  +  I  +N+    K+G       LF FP     +G ++    + + +E        
Sbjct: 190 LKLTQHHIQRRNEGGVLKEGMTGNTYNLFAFPSECNFSGRKFDPNLIKIIKEGSERILES 249

Query: 317 -------WHVLVDA---CALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKK 376
                  W VL+DA   CA  P +     LS+F+ DF+V SFYK+FG  P+G G L+V+K
Sbjct: 250 SQYSRGCWLVLIDAAKGCATNPPN-----LSMFKADFVVFSFYKLFG-YPTGLGALIVRK 309

Query: 377 SVISILEGNSCSSNVGIVNLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSG 436
                                 A KL++    S GT           ++  +     F  
Sbjct: 310 D---------------------AAKLMKKTYFSGGT-----------VTAAIADVDFF-- 369

Query: 437 PISHPSMKTRTQFPEMEEEGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARC 496
                  K R    E  E+G +      I A+         G   ++ L    I      
Sbjct: 370 -------KRREGVEEFFEDGTI--SFLSITAIQ-------HGFKIINMLTTSSIFRHTTS 429

Query: 497 LINWLVSSLLKLKHPNSEGVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLAD 556
           +  ++ + LL LKH N E VC   +YG  +  + GP ++FN+    G       V+KLA 
Sbjct: 430 IAAYVRNKLLALKHENGEFVC--TLYG-LLSSEMGPTVSFNMKRPDGTWYGYREVEKLAT 489

Query: 557 RSNISLSYGFLHNIWFSDKY-AEEKGKVLERKESG----KDEKLKKAKANLGISVVTAAL 603
            + I L  G   N     KY       +L   E+G     D  +   K       V  + 
Sbjct: 490 LAGIQLRTGCFCNPGACAKYLGLSHLDLLSNIEAGHVCWDDRDILHGKPT---GAVRVSF 493

BLAST of Spg034393 vs. ExPASy Swiss-Prot
Match: A1CHL0 (Molybdenum cofactor sulfurase OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1) OX=344612 GN=hxB PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 3.9e-15
Identity = 132/529 (24.95%), Postives = 211/529 (39.89%), Query Frame = 0

Query: 87  TQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYHQFQKHPNPFPSSNLNFPFFGVSYKSG 146
           ++++D IR ++Y    L + T LD+ G  L++    +       +SNL           G
Sbjct: 19  SEDVDIIREREY--PQLKDTTYLDHAGTTLYAKSLIEAFSRDL-TSNL----------FG 78

Query: 147 NLKSRLLENALDSDLESAVKRRIFRFLNVSESDYAMVFTANRTSAFKLLAESYPFQTSNK 206
           N  S    + L +     V+ R  RF      D+ +VF AN T+A KL+A++      ++
Sbjct: 79  NPHSMSASSQLSTRRVDDVRLRALRFFKADPDDFDLVFVANATAAIKLVADA---MRDSR 138

Query: 207 LLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWPRLRINSRKLKEMIVSKNKKKKAKKGL 266
               Y Y  +A  ++V      GA  ++A+ +  R   +  +++  I S  +       L
Sbjct: 139 QGFWYGYHVDAHTSLV------GARELAAKGN--RCFSSDEEVEGWIQSLREAGPESLNL 198

Query: 267 FVFPLHSRITGARYPYLW------MSVAQENRWHVLVDACAL---GPKDMDCFGLSLFRP 326
           F +P  S + G R P  W       S A     + L+DA +L    P D+     +   P
Sbjct: 199 FAYPAQSNLNGRRLPLSWCETIRRRSEAAGGNTYTLLDAASLVSTSPLDLSDAAAA---P 258

Query: 327 DFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIVNLVPADKLLQLAEDSSG 386
           DF V SFYK+FG      G L+V+KS   I E        G V++V   ++   A+  S 
Sbjct: 259 DFTVLSFYKIFGF--PDLGALIVRKSAGHIFEQRRFFGG-GTVDMVLTREMQWHAKKQSS 318

Query: 387 TDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKTRTQFPEMEEEGNLGSKVSEIEAVSKQ 446
               LE     F S ++   S+F+   +H     R  F  ME                  
Sbjct: 319 IHDRLEDGTLPFHS-IIALDSAFA---TH-----RRLFGSMEN----------------- 378

Query: 447 LETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKHPNSEGVCLVKIY-GPKVKFDR 506
                             +S+  R L   L   L  LKH N E VC  ++Y  P   +++
Sbjct: 379 ------------------VSSHTRFLAKRLYDKLAALKHSNGERVC--QLYTNPFSDYNK 438

Query: 507 ----GPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSDKYAEEKGKVLER 566
               GP +AFN+ +  G  +    V++LA   NI    G L N   +       G  L +
Sbjct: 439 AASQGPIIAFNLRNSHGAWIGKSEVERLATVKNIQFRSGSLCNPGGTSGSLGWTGADLLQ 468

Query: 567 KES-----GKDEKLKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQF 597
           + S     G D  +   +      V+  +LG +TN  D+  +  FV +F
Sbjct: 499 QFSAGLRCGDDHDVMDGRPT---GVLRLSLGAMTNLADINTVIQFVEEF 468

BLAST of Spg034393 vs. ExPASy Swiss-Prot
Match: A2QIK9 (Molybdenum cofactor sulfurase OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=hxB PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 8.8e-15
Identity = 122/528 (23.11%), Postives = 202/528 (38.26%), Query Frame = 0

Query: 89  EIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYHQFQKHPNPFPSSNLNFPFFGVSYKSGNL 148
           ++D IR ++Y    L + T LD+ G  L++    +       +SNL           GN 
Sbjct: 12  DVDVIREREY--PLLKDTTYLDHAGTTLYAKSLIESFSRDL-TSNL----------YGNP 71

Query: 149 KSRLLENALDSDLESAVKRRIFRFLNVSESDYAMVFTANRTSAFKLLAESYPFQTSNKLL 208
            S    + L +     ++ R  RF +    ++ +VF AN T+A KL+A+S  F+ S    
Sbjct: 72  HSMSAPSQLSTQRVDDIRLRALRFFSADPEEFDLVFVANATAAIKLVADS--FRESTPQG 131

Query: 209 TVYDYESEAVEAMVSTSQNRGALTMSAEFSWPRLRINSRKLKEMIVSKNKKKKAKKGLFV 268
             Y Y  ++  ++V   +  G           R  +   +++  I   + +      LF 
Sbjct: 132 FWYGYHVDSHTSLVGARELAGI--------GSRCFVTDAEVESWISQLDTEPVQGPRLFA 191

Query: 269 FPLHSRITGARYPYLWMSVAQENR--WHVLVDACALGPKDMDCFGLSLFRPDFLVCSFYK 328
           +P  S + G R+P  W    +E+    + L+D  +L          +   PDF V SFYK
Sbjct: 192 YPAQSNMNGRRFPRGWCGRIRESAKDTYTLLDVASLVSTSPFDLSDASAAPDFAVLSFYK 251

Query: 329 VFGENPSGFGCLLVKKSVISILEGNSCSSNVGIVNLVPADKLLQLAEDSSGTDTELEIQH 388
           +FG      G L+V+KS   I +        G V++V           + GT        
Sbjct: 252 IFGF--PDLGALIVRKSAGHIFDKRKFFGG-GTVDMVL----------TQGTQWH----- 311

Query: 389 RQFMSQMVPSTSSFSGPISHPSMKTRTQFPEMEEEGNLGSKVSEIEAVSKQLETKCRGLD 448
                                  K ++   E  E+G L      I A+    +T  R   
Sbjct: 312 ----------------------AKKQSSIHERLEDGTL--PFHNIIALGSAFDTHERLFG 371

Query: 449 QVDSLGLILISTRARCLINWLVSSLLKLKHPNSEGVCLVKIYGPKVK-----FDRGPALA 508
            +D+     IS+  R L   L   +  L+H N E VC   +Y P          +GP LA
Sbjct: 372 SMDN-----ISSHTRFLAKRLYDRMTTLRHYNGESVC--HVYKPSHSDYTDPSTQGPILA 431

Query: 509 FNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSDKYAEEKGKVLER-----KESG 568
           FN+   +G  +    V+K+A   NI +  G L N   +       G  + R        G
Sbjct: 432 FNLRSSQGAWIGKSEVEKMASVRNIQIRSGTLCNPGGTAASLNWSGADMLRHFGAGMRCG 460

Query: 569 KDEKLKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQFLDADFVEK 605
            D  +   +      ++  +LG ++N  D+     F+ +F    +VEK
Sbjct: 492 DDHDIMDGRPT---GILRVSLGAMSNLTDIDTFMGFIEEF----YVEK 460

BLAST of Spg034393 vs. ExPASy Swiss-Prot
Match: Q14CH1 (Molybdenum cofactor sulfurase OS=Mus musculus OX=10090 GN=Mocos PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.3e-14
Identity = 125/560 (22.32%), Postives = 217/560 (38.75%), Query Frame = 0

Query: 69  PPPQALSLFLAAFPQY----HETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYHQFQK 128
           P P A    L A  Q     +  + + ++R++++    L+    LD+ G  L        
Sbjct: 10  PEPPAFQRHLEASTQRLAHGYGLRSMSELRDQEFG--RLAGTVYLDHAGATL-------- 69

Query: 129 HPNPFPSSNL-NFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDYAMV 188
               FP S L NF    +    GN  S+ + + L  D    V+ RI    + +  DY ++
Sbjct: 70  ----FPQSQLTNFTKDLMENVYGNPHSQNITSKLTHDTVEQVRYRILTHFHTTPEDYIVI 129

Query: 189 FTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWPRLR 248
           FTA  T+A +L+AE++P+ + +        E+        T  +   + M    +   + 
Sbjct: 130 FTAGSTAALRLVAEAFPWVSRSP-------ENSGSHFCYLTDNHTSVVGMRKVAAAMSVT 189

Query: 249 INSRKLKEMIVSKNKKKKA------KKGLFVFPLHSRITGARYPYLWMSVAQENR----- 308
               K ++M  ++ K   A         LF +P  S  +G RYP  W+   +  R     
Sbjct: 190 SIPVKPEDMWSAEGKDAGACDPDCQLPHLFCYPAQSNFSGTRYPLSWVEEVKSGRRSPVN 249

Query: 309 ----WHVLVDACA-LGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISI 368
               W VL+DA + +    +D   LS  + DF+  SFYK+FG  P+G G LLV K V  +
Sbjct: 250 APGKWFVLLDAASYVSTSPLD---LSAHQADFIPISFYKIFG-LPTGLGALLVNKHVAPL 309

Query: 369 LEGNSCSSNVGIVNLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHP 428
           L             L   D                                 F  P    
Sbjct: 310 LRKGYFGGGTAAAYLAGED---------------------------------FYVP---- 369

Query: 429 SMKTRTQFPEMEEEGNLGSKVSEIEAVSKQLETKCRGLDQVDSL--GLILISTRARCLIN 488
               R+   E  E+G     +S ++ ++ +      G D ++ L  G++ I      L+ 
Sbjct: 370 ----RSSVAERFEDGT----ISFLDVIALK-----HGFDALEHLTGGMVNIQQHTFALVQ 429

Query: 489 WLVSSLLKLKHPNSEGVCLVKIY------GPKVKFDRGPALAFNVFDWKGEKVEPVLVQK 548
           +  S+L  L++ N  G  +V+IY       P V   +GP + FNV D  G+ +    V K
Sbjct: 430 YTHSALSSLRYLN--GAPVVRIYSDSEFSSPDV---QGPIINFNVLDDGGKIIGYSQVDK 486

Query: 549 LADRSNISLSYGFLHNIWFSDKYAEEKGKVLER-----KESGKDEKLKKAKANLGISVVT 595
           +A   NI L  G   N+    ++     +++++        G D  +   +    + +  
Sbjct: 490 MASLYNIHLRTGCFCNLGACQRHLGLSDEMVKKHFQAGHVCGDDVDIIDGRPTGSVRI-- 486

BLAST of Spg034393 vs. ExPASy TrEMBL
Match: A0A6J1GZR0 (molybdenum cofactor sulfurase OS=Cucurbita moschata OX=3662 GN=LOC111459100 PE=4 SV=1)

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 557/625 (89.12%), Postives = 587/625 (93.92%), Query Frame = 0

Query: 1   MATACGGGRGGGSSCCPTPLLNFPSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTN 60
           +A ACGGG GGGSSCCPTPLLNFPSSTSTT+TT   PRTSAASRRDF AKA+AGVFPNTN
Sbjct: 9   IAAACGGGSGGGSSCCPTPLLNFPSSTSTTSTT---PRTSAASRRDFTAKATAGVFPNTN 68

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           FTNPE+LP PPQAL+LFLAAFPQY++TQ+IDQIRNKQYYHLN +NHTCLDYIGIGLFSYH
Sbjct: 69  FTNPESLPAPPQALALFLAAFPQYNQTQKIDQIRNKQYYHLNFTNHTCLDYIGIGLFSYH 128

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDY 180
           QFQKH NPFPSSNLNFPFFGVSY++GN KSRLLEN LDSDLESA+KRRIFRFLNVS+SDY
Sbjct: 129 QFQKHHNPFPSSNLNFPFFGVSYRTGNFKSRLLENGLDSDLESAIKRRIFRFLNVSDSDY 188

Query: 181 AMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 240
           +MVFTANRTSAFKLLAESYPF TS+KLLTVYDYESEAVEAMV+TSQNRGALTMSAEFSWP
Sbjct: 189 SMVFTANRTSAFKLLAESYPFHTSSKLLTVYDYESEAVEAMVNTSQNRGALTMSAEFSWP 248

Query: 241 RLRINSRKLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDAC 300
           RLRINSRKLKEMIVSKNKKKK KKGLFVFPLHSRITGARYPYLWMS+AQENRWHVLVDAC
Sbjct: 249 RLRINSRKLKEMIVSKNKKKKTKKGLFVFPLHSRITGARYPYLWMSIAQENRWHVLVDAC 308

Query: 301 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIV 360
           ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISIL+ NSCSSNVGIV
Sbjct: 309 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILDTNSCSSNVGIV 368

Query: 361 NLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKT----RTQFP 420
           NLVPADKL QLAEDSSGTDTELEI H+QFMSQMVPSTSSFSGPIS+ SMKT     TQFP
Sbjct: 369 NLVPADKLFQLAEDSSGTDTELEIHHQQFMSQMVPSTSSFSGPISYESMKTAQTPTTQFP 428

Query: 421 EMEEEGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKH 480
           E EE+ NLGS VSEI       ETKC+GLDQ+DSLGLILISTRARCLINWLVSSLLKLKH
Sbjct: 429 ETEEDPNLGSNVSEI-------ETKCKGLDQMDSLGLILISTRARCLINWLVSSLLKLKH 488

Query: 481 PNSEGVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNI 540
           PN+EGV LVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFL NI
Sbjct: 489 PNTEGVSLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLQNI 548

Query: 541 WFSDKYAEEKGKVLERKESGKDEKLKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQF 600
           WF +KYAEEKG+VLERKE GKDEK+KK KANLGISVVTAALGFLTNF+DVYKLW FVAQF
Sbjct: 549 WFPEKYAEEKGRVLERKEVGKDEKMKKVKANLGISVVTAALGFLTNFDDVYKLWCFVAQF 608

Query: 601 LDADFVEKERWRYTALNQRTIIEVL 622
           LDADFVEKERWRYTALNQRT++EVL
Sbjct: 609 LDADFVEKERWRYTALNQRTVLEVL 623

BLAST of Spg034393 vs. ExPASy TrEMBL
Match: A0A6J1JYG9 (molybdenum cofactor sulfurase OS=Cucurbita maxima OX=3661 GN=LOC111490890 PE=4 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 557/625 (89.12%), Postives = 585/625 (93.60%), Query Frame = 0

Query: 1   MATACGGGRGGGSSCCPTPLLNFPSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTN 60
           +A ACGGG GGGSSCCPTPLLNFPSSTSTT+TT   PRTSAASRRDF AKA AGVFPNTN
Sbjct: 9   IAAACGGGSGGGSSCCPTPLLNFPSSTSTTSTT---PRTSAASRRDFTAKAIAGVFPNTN 68

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           FTNPE+LP PPQALSLFLAAFPQY++TQ+IDQIRNKQYYHLN +NHTCLDYIGIGLFSYH
Sbjct: 69  FTNPESLPGPPQALSLFLAAFPQYNQTQKIDQIRNKQYYHLNFTNHTCLDYIGIGLFSYH 128

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDY 180
           QFQKH NPFPSSNLNFPFFGVSY++GN KSRLLEN LDSDLESA+KRRIFRFLNVS+SDY
Sbjct: 129 QFQKHHNPFPSSNLNFPFFGVSYRTGNFKSRLLENGLDSDLESAIKRRIFRFLNVSDSDY 188

Query: 181 AMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 240
           +MVFTANRTSAFKLLAESYPF TSNKLLTVYDYESEAVEAMV+TSQNRGALTMSAEFSWP
Sbjct: 189 SMVFTANRTSAFKLLAESYPFHTSNKLLTVYDYESEAVEAMVNTSQNRGALTMSAEFSWP 248

Query: 241 RLRINSRKLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDAC 300
           RLRINSRKLKEMIVSKNKKKK  KGLFVFPLHSRITGARYPYLWMS+AQENRWHVLVDAC
Sbjct: 249 RLRINSRKLKEMIVSKNKKKKTNKGLFVFPLHSRITGARYPYLWMSIAQENRWHVLVDAC 308

Query: 301 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIV 360
           ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKK+VISIL+ NSCSSNVGIV
Sbjct: 309 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKTVISILDTNSCSSNVGIV 368

Query: 361 NLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKT----RTQFP 420
           NLVPADKL QLAEDSSGTDTELEI H+QFMSQMVPSTSSFSGPIS+ SMKT     TQFP
Sbjct: 369 NLVPADKLFQLAEDSSGTDTELEIHHQQFMSQMVPSTSSFSGPISYESMKTAQTSTTQFP 428

Query: 421 EMEEEGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKH 480
           E EE+ NLGS VSEI       ETKC+GLDQ+DSLGLILISTRARCLINWLVSSLLKLKH
Sbjct: 429 ETEEDPNLGSNVSEI-------ETKCKGLDQMDSLGLILISTRARCLINWLVSSLLKLKH 488

Query: 481 PNSEGVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNI 540
           PN+EGV LVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFL NI
Sbjct: 489 PNTEGVSLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLQNI 548

Query: 541 WFSDKYAEEKGKVLERKESGKDEKLKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQF 600
           WF +KYAEEKG+VLERKE GKDEK+KK KANLGISVVTAALGFLTNF+DVYKLW FVAQF
Sbjct: 549 WFPEKYAEEKGRVLERKEVGKDEKMKKVKANLGISVVTAALGFLTNFDDVYKLWCFVAQF 608

Query: 601 LDADFVEKERWRYTALNQRTIIEVL 622
           LDADFVEKERWRYTALNQRT++EVL
Sbjct: 609 LDADFVEKERWRYTALNQRTVLEVL 623

BLAST of Spg034393 vs. ExPASy TrEMBL
Match: A0A5D3BQC4 (Molybdenum cofactor sulfurase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00550 PE=4 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 554/622 (89.07%), Postives = 576/622 (92.60%), Query Frame = 0

Query: 1   MATACGGGRGGGSSCCPTPLLNFPSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTN 60
           ++ ACGGG GGGSSCCPTPLLNFPSS  +TT T   PRTSAASRRDFAAKASAGVFPNT 
Sbjct: 9   ISAACGGGSGGGSSCCPTPLLNFPSSQPSTTAT---PRTSAASRRDFAAKASAGVFPNTT 68

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           FTNPE LP PPQALSLFLAAFPQY +TQEID IRN+QYYHLNLSNH CLDYIGIGLFSYH
Sbjct: 69  FTNPECLPSPPQALSLFLAAFPQYSQTQEIDAIRNRQYYHLNLSNHICLDYIGIGLFSYH 128

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDY 180
           QFQKH NPFPSSNLNFPFFGVSY++GNLKSRLLEN LDSDLESA+KRRIFRFLNVSESDY
Sbjct: 129 QFQKHSNPFPSSNLNFPFFGVSYRTGNLKSRLLENGLDSDLESAIKRRIFRFLNVSESDY 188

Query: 181 AMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 240
           AM+FTANRTSAFKLLAESYPFQTSNK+LTVYDYESEAVEAMVS+SQNRGA TMSAEFSWP
Sbjct: 189 AMIFTANRTSAFKLLAESYPFQTSNKVLTVYDYESEAVEAMVSSSQNRGATTMSAEFSWP 248

Query: 241 RLRINSRKLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDAC 300
           RLRINSRKLKEMIVSKNKKKK+KKGLFVFPLHSRITGARYPYLWMS+AQENRWHVLVDAC
Sbjct: 249 RLRINSRKLKEMIVSKNKKKKSKKGLFVFPLHSRITGARYPYLWMSIAQENRWHVLVDAC 308

Query: 301 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIV 360
           ALGPKDMDCFGLSLFRPDFLV SFYKVFGENPSGFGCLLVKKSVISILE NS SSNVGIV
Sbjct: 309 ALGPKDMDCFGLSLFRPDFLVSSFYKVFGENPSGFGCLLVKKSVISILETNSSSSNVGIV 368

Query: 361 NLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKTRTQFPEMEE 420
           NLVPADKLLQL EDSSGTD +LE Q +    QMVPSTSSFSGPISH   KT T F EM+E
Sbjct: 369 NLVPADKLLQLNEDSSGTDIDLEFQQQ----QMVPSTSSFSGPISHQISKTTTNFSEMDE 428

Query: 421 EGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKHPNSE 480
           EG  GS VSEIE VS + ETKC+GLDQVDSLGL+LISTRARCLINWLVSSLLKLKHPNS+
Sbjct: 429 EGKSGSNVSEIEIVSNRYETKCKGLDQVDSLGLVLISTRARCLINWLVSSLLKLKHPNSQ 488

Query: 481 GVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSD 540
           G  LVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSD
Sbjct: 489 GASLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSD 548

Query: 541 KYAEEKGKVLERKESGKDEK-LKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQFLDA 600
           KYAEEKGKVLERKE GKDEK +KK+K NLGISVVTAALGFLTNFEDVYKLW FVAQFLDA
Sbjct: 549 KYAEEKGKVLERKEFGKDEKNMKKSKGNLGISVVTAALGFLTNFEDVYKLWGFVAQFLDA 608

Query: 601 DFVEKERWRYTALNQRTIIEVL 622
           DFVEKERWRYTALNQRTIIEVL
Sbjct: 609 DFVEKERWRYTALNQRTIIEVL 623

BLAST of Spg034393 vs. ExPASy TrEMBL
Match: A0A1S3CT46 (molybdenum cofactor sulfurase OS=Cucumis melo OX=3656 GN=LOC103504647 PE=4 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 554/622 (89.07%), Postives = 576/622 (92.60%), Query Frame = 0

Query: 1   MATACGGGRGGGSSCCPTPLLNFPSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTN 60
           ++ ACGGG GGGSSCCPTPLLNFPSS  +TT T   PRTSAASRRDFAAKASAGVFPNT 
Sbjct: 9   ISAACGGGSGGGSSCCPTPLLNFPSSQPSTTAT---PRTSAASRRDFAAKASAGVFPNTT 68

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           FTNPE LP PPQALSLFLAAFPQY +TQEID IRN+QYYHLNLSNH CLDYIGIGLFSYH
Sbjct: 69  FTNPECLPSPPQALSLFLAAFPQYSQTQEIDAIRNRQYYHLNLSNHICLDYIGIGLFSYH 128

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDY 180
           QFQKH NPFPSSNLNFPFFGVSY++GNLKSRLLEN LDSDLESA+KRRIFRFLNVSESDY
Sbjct: 129 QFQKHSNPFPSSNLNFPFFGVSYRTGNLKSRLLENGLDSDLESAIKRRIFRFLNVSESDY 188

Query: 181 AMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 240
           AM+FTANRTSAFKLLAESYPFQTSNK+LTVYDYESEAVEAMVS+SQNRGA TMSAEFSWP
Sbjct: 189 AMIFTANRTSAFKLLAESYPFQTSNKVLTVYDYESEAVEAMVSSSQNRGATTMSAEFSWP 248

Query: 241 RLRINSRKLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDAC 300
           RLRINSRKLKEMIVSKNKKKK+KKGLFVFPLHSRITGARYPYLWMS+AQENRWHVLVDAC
Sbjct: 249 RLRINSRKLKEMIVSKNKKKKSKKGLFVFPLHSRITGARYPYLWMSIAQENRWHVLVDAC 308

Query: 301 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIV 360
           ALGPKDMDCFGLSLFRPDFLV SFYKVFGENPSGFGCLLVKKSVISILE NS SSNVGIV
Sbjct: 309 ALGPKDMDCFGLSLFRPDFLVSSFYKVFGENPSGFGCLLVKKSVISILETNSSSSNVGIV 368

Query: 361 NLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKTRTQFPEMEE 420
           NLVPADKLLQL EDSSGTD +LE Q +    QMVPSTSSFSGPISH   KT T F EM+E
Sbjct: 369 NLVPADKLLQLNEDSSGTDIDLEFQQQ----QMVPSTSSFSGPISHQISKTTTNFSEMDE 428

Query: 421 EGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKHPNSE 480
           EG  GS VSEIE VS + ETKC+GLDQVDSLGL+LISTRARCLINWLVSSLLKLKHPNS+
Sbjct: 429 EGKSGSNVSEIEIVSNRYETKCKGLDQVDSLGLVLISTRARCLINWLVSSLLKLKHPNSQ 488

Query: 481 GVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSD 540
           G  LVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSD
Sbjct: 489 GASLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSD 548

Query: 541 KYAEEKGKVLERKESGKDEK-LKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQFLDA 600
           KYAEEKGKVLERKE GKDEK +KK+K NLGISVVTAALGFLTNFEDVYKLW FVAQFLDA
Sbjct: 549 KYAEEKGKVLERKEFGKDEKNMKKSKGNLGISVVTAALGFLTNFEDVYKLWGFVAQFLDA 608

Query: 601 DFVEKERWRYTALNQRTIIEVL 622
           DFVEKERWRYTALNQRTIIEVL
Sbjct: 609 DFVEKERWRYTALNQRTIIEVL 623

BLAST of Spg034393 vs. ExPASy TrEMBL
Match: A0A0A0KRQ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G172810 PE=4 SV=1)

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 554/623 (88.92%), Postives = 576/623 (92.46%), Query Frame = 0

Query: 1   MATACGGGRGGGSSCCPTPLLNFPSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTN 60
           ++ ACGGG GGGSSCCPTPLLNFPSS  +TT T   PRTSAASRRDFAAKASAGVFPNT 
Sbjct: 9   ISAACGGGSGGGSSCCPTPLLNFPSSQPSTTAT---PRTSAASRRDFAAKASAGVFPNTT 68

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           FTNPE LP PPQALSLFLAAFPQY +TQEID IRN+QYYHLNLSNH CLDYIGIGLFSYH
Sbjct: 69  FTNPECLPSPPQALSLFLAAFPQYSQTQEIDAIRNRQYYHLNLSNHICLDYIGIGLFSYH 128

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDY 180
           QFQKH NPFPSSNLNFPFFGVSY++GNLKSRLLEN LDSDLESA+KRRIFRFLNVSESDY
Sbjct: 129 QFQKHSNPFPSSNLNFPFFGVSYRTGNLKSRLLENGLDSDLESAIKRRIFRFLNVSESDY 188

Query: 181 AMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWP 240
           AM+FTANRTSAFKLLAESYPFQTSNK+LTVYDYESEAVEAMVS+SQNRGA TMSAEFSWP
Sbjct: 189 AMIFTANRTSAFKLLAESYPFQTSNKVLTVYDYESEAVEAMVSSSQNRGATTMSAEFSWP 248

Query: 241 RLRINSRKLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDAC 300
           RLRINSRKLKEMIVSKNKKKK KKGLFVFPLHSRITGARYPYLWMS+AQENRWHVLVDAC
Sbjct: 249 RLRINSRKLKEMIVSKNKKKKTKKGLFVFPLHSRITGARYPYLWMSIAQENRWHVLVDAC 308

Query: 301 ALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIV 360
           ALGPKDMDCFGLSLFRPDFLV SFYKVFGENPSGFGCLLVKKSVISILE NS SSNVGIV
Sbjct: 309 ALGPKDMDCFGLSLFRPDFLVSSFYKVFGENPSGFGCLLVKKSVISILETNSSSSNVGIV 368

Query: 361 NLVPADKLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHP-SMKTRTQFPEME 420
           NLVPADKLLQL EDSSGTD +LE Q +    QMVPSTSSFSGPISH  S  T T FPEM+
Sbjct: 369 NLVPADKLLQLNEDSSGTDIDLEFQQQ----QMVPSTSSFSGPISHQISKTTTTNFPEMD 428

Query: 421 EEGNLGSKVSEIEAVSKQLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKHPNS 480
           EEG   S VSEIE VS + ETKC+GLDQVDSLGL+LISTRARCLINWLVSSLLKLKHPNS
Sbjct: 429 EEGKSRSNVSEIEIVSNRYETKCKGLDQVDSLGLVLISTRARCLINWLVSSLLKLKHPNS 488

Query: 481 EGVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFS 540
           +GVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNI FS
Sbjct: 489 QGVCLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNICFS 548

Query: 541 DKYAEEKGKVLERKESGKDEK-LKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQFLD 600
           DKY EEKGKVLERKE GKDEK +KK+K NLGISVVTAALGFLTNFEDVYKLW+FVAQFLD
Sbjct: 549 DKYGEEKGKVLERKEFGKDEKNMKKSKGNLGISVVTAALGFLTNFEDVYKLWSFVAQFLD 608

Query: 601 ADFVEKERWRYTALNQRTIIEVL 622
           ADFVEKERWRYTALNQRTIIEVL
Sbjct: 609 ADFVEKERWRYTALNQRTIIEVL 624

BLAST of Spg034393 vs. TAIR 10
Match: AT5G51920.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 551.6 bits (1420), Expect = 8.1e-157
Identity = 308/592 (52.03%), Postives = 395/592 (66.72%), Query Frame = 0

Query: 23  FPSSTSTTTTTTAAPR-TSAASRRDFAAKASAGVFPNTNFTNPETLPPPPQALSLFLAAF 82
           F  +TS+     + P  TSA  RR+FA    + +FP+T FT+P +LP   ++ S F+ A+
Sbjct: 24  FHGTTSSEHPPHSTPTVTSATLRRNFAQTTVSTIFPDTEFTDPNSLPSHQESFSDFIQAY 83

Query: 83  PQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYHQFQKH-PNPF--PSSNLNFPF 142
           P Y +T +ID++R+  Y+HL LS++TCLDYIGIGL+SY Q   + P+ +   SS    PF
Sbjct: 84  PNYSDTYKIDRLRSDHYFHLGLSHYTCLDYIGIGLYSYSQLLNYDPSTYQISSSLSESPF 143

Query: 143 FGVSYKSGNLKSRLL-ENALDSDLESAVKRRIFRFLNVSESDYAMVFTANRTSAFKLLAE 202
           F VS K GNLK +LL +   +++ E ++KRRI  FL +SE DY+MVFTANRTSAF+L+AE
Sbjct: 144 FSVSPKIGNLKEKLLNDGGQETEFEYSMKRRIMGFLKISEEDYSMVFTANRTSAFRLVAE 203

Query: 203 SYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWPRLRINSRKLKEMIVS-K 262
           SYPF +  KLLTVYDYESEAV  +   S+ RGA   +AEFSWPRL++ S KL++++ + K
Sbjct: 204 SYPFNSKRKLLTVYDYESEAVSEINRVSEKRGAKVAAAEFSWPRLKLCSSKLRKLVTAGK 263

Query: 263 NKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDACALGPKDMDCFGLSLFR 322
           N  K  KKG++VFPLHSR+TG+RYPYLWMSVAQEN WHV++DAC LGPKDMD FGLS++ 
Sbjct: 264 NGSKTKKKGIYVFPLHSRVTGSRYPYLWMSVAQENGWHVMIDACGLGPKDMDSFGLSIYN 323

Query: 323 PDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIVNLVPADKLLQLAEDSS 382
           PDF+VCSFYKVFGENPSGFGCL VKKS ISILE    S+  G++NLVP D  + L     
Sbjct: 324 PDFMVCSFYKVFGENPSGFGCLFVKKSTISILES---STGPGMINLVPTDNPISL----- 383

Query: 383 GTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKTRTQFPEMEEEGNLGSKVSEIEAVSK 442
                LEI   Q  S+    T SFS  + +                              
Sbjct: 384 ---HALEINRTQTDSE---ETYSFSSSVEY------------------------------ 443

Query: 443 QLETKCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKHPNSEGVCLVKIYGPKVKFDR 502
                 +GLD VDSLGL+    R+RCLINWLVS+L KLKH  +    LVKIYGPKV F+R
Sbjct: 444 ------KGLDHVDSLGLVATGNRSRCLINWLVSALYKLKHSTTSR--LVKIYGPKVNFNR 503

Query: 503 GPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSDKYAEEKGKVLERKESG 562
           GPA+AFN+F+ KGEK+EP +VQKLA+ SNISL   FL NI F + Y   K +V E+K + 
Sbjct: 504 GPAVAFNLFNHKGEKIEPFIVQKLAECSNISLGKSFLKNILFQEDYEGVKDRVFEKKRNR 556

Query: 563 KDEKLKKAKANLGISVVTAALGFLTNFEDVYKLWAFVAQFLDADFVEKERWR 609
             ++ +       ISV+TAALGFL NFEDVYKLW FVA+FLD++FV+KE  R
Sbjct: 564 DVDEPR-------ISVLTAALGFLANFEDVYKLWIFVARFLDSEFVDKESVR 556

BLAST of Spg034393 vs. TAIR 10
Match: AT4G22980.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 9 growth stages; BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT5G51920.1); Has 520 Blast hits to 468 proteins in 130 species: Archae - 5; Bacteria - 23; Metazoa - 99; Fungi - 131; Plants - 231; Viruses - 0; Other Eukaryotes - 31 (source: NCBI BLink). )

HSP 1 Score: 387.5 bits (994), Expect = 2.0e-107
Identity = 243/613 (39.64%), Postives = 345/613 (56.28%), Query Frame = 0

Query: 13  SSCCPTPLLNF-----PSSTSTTTTTTAAPRTSAASRRDFAAKASAGVFPNTNFTNPETL 72
           + CC +P  +      P     + TTT    TS  +R             NT FT+ E+L
Sbjct: 15  NGCCSSPFSSHSMSEKPEELEFSVTTTG---TSFLTR-------------NTKFTSQESL 74

Query: 73  PPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYHQFQKHPN 132
           P    +    + AFP Y +T + D +R+ +Y +L+ S+H         LFSY QF++   
Sbjct: 75  PRLRTSFYDLITAFPDYLQTNQADHLRSTEYQNLSSSSHVFGQQ--QPLFSYSQFREISE 134

Query: 133 PFPSSNLNFPFFGVSYKSGNLKSRLLENALDSDLESAVKRRIFRFLNVSESDYAMVFTAN 192
               S+LN     +S K  +    LL    +S  +S +++RI  F+N+ ES+Y M+ T +
Sbjct: 135 --SESDLNHSLLTLSCKQVSSGKELLSFEEESRFQSRIRKRITSFMNLEESEYHMILTQD 194

Query: 193 RTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWPRLRINSR 252
           R+SAFK++AE Y F+T+  LLTVY+YE EAVE M+  S+ +G    SAEFSWP   I S 
Sbjct: 195 RSSAFKIVAELYSFKTNPNLLTVYNYEDEAVEEMIRISEKKGIKPQSAEFSWPSTEILSE 254

Query: 253 KLKEMIVSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDACALGPKDM 312
           KLK  I     K++ K+GLFVFPL S +TGA Y Y WMS+A+E+ WHVL+D  ALG KDM
Sbjct: 255 KLKRRIT--RSKRRGKRGLFVFPLQSLVTGASYSYSWMSLARESEWHVLLDTSALGSKDM 314

Query: 313 DCFGLSLFRPDFLVCSFYKVFG-ENPSGFGCLLVKKSVISILEGNSCSSNVGIVNLVPAD 372
           +  GLSLF+PDFL+CSF +V G ++PSGFGCL VKKS  + L     +         P +
Sbjct: 315 ETLGLSLFQPDFLICSFTEVLGQDDPSGFGCLFVKKSSSTALSEEPTN---------PEN 374

Query: 373 KLLQLAEDSSGTDTELEIQHRQFMSQMVPSTSSFSGPISHPSMKTRTQFPEMEEEGNLGS 432
                AE S    TE +  + +              P+ H   K  +            S
Sbjct: 375 LTAVKAEPSWKWKTEYQAGYNEIT------------PVDHEDHKAAS---------TSSS 434

Query: 433 KVSEIEAVSKQLET--KCRGLDQVDSLGLILISTRARCLINWLVSSLLKLKHP--NSEGV 492
           ++ EIE+  KQ +   + +GLD  DSLGLILIS R++ L  WL+ +L  L+HP  +   +
Sbjct: 435 EIVEIESSVKQDKAMIEFQGLDHADSLGLILISRRSKSLTLWLLRALRTLQHPGYHQTEM 494

Query: 493 CLVKIYGPKVKFDRGPALAFNVFDWKGEKVEPVLVQKLADRSNISLSYGFLHNIWFSDKY 552
            LVK+YGPK K  RGP+++FN+FDW+GEKV+P++V++LA+R  I L   +LH     +K 
Sbjct: 495 PLVKLYGPKTKPSRGPSISFNIFDWQGEKVDPLMVERLAEREKIGLRCAYLHKFRIGNKR 554

Query: 553 AEEKGKVLERKESGKDEKLKKAKANLGISVVTAAL-GFLTNFEDVYKLWAFVAQFLDADF 612
             ++                    +L +SVVT  L GF+TNFEDV+K+W FV++FLDADF
Sbjct: 555 RSDEA------------------VSLRLSVVTVRLGGFMTNFEDVFKVWEFVSRFLDADF 557

Query: 613 VEKERWRYTALNQ 615
           VEKE+WR  AL++
Sbjct: 615 VEKEKWRMKALDK 557

BLAST of Spg034393 vs. TAIR 10
Match: AT5G66950.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 352.8 bits (904), Expect = 5.5e-97
Identity = 253/769 (32.90%), Postives = 357/769 (46.42%), Query Frame = 0

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           F + ETLP   +AL++FL  +P+Y  ++++D++RN +Y+HL+L    CLDY G GLFSY 
Sbjct: 102 FESEETLPELEEALTIFLTMYPKYQSSEKVDELRNDEYFHLSLPK-VCLDYCGFGLFSYL 161

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALD-SDLESAVKRRIFRFLNVSESD 180
           Q   + +           F +S  S NL +  +    +   +E  +K RI  +LN+ E++
Sbjct: 162 QTVHYWDTCT--------FSLSEISANLSNHAIYGGAEKGSIEHDIKIRIMDYLNIPENE 221

Query: 181 YAMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSW 240
           Y +VFT +R SAFKLLAESYPF T+ KLLT++D+ES++V  M   ++ +GA   SA F W
Sbjct: 222 YGLVFTVSRGSAFKLLAESYPFHTNKKLLTMFDHESQSVSWMGQCAKEKGAKVGSAWFKW 281

Query: 241 PRLRINSRKLKEMIVSKNKKKK-AKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVD 300
           P LR+ S  LK+ I+SK K+KK +  GLFVFP+ SR+TG++Y Y WM++AQ+N WHVL+D
Sbjct: 282 PTLRLCSMDLKKEILSKKKRKKDSATGLFVFPVQSRVTGSKYSYQWMALAQQNNWHVLLD 341

Query: 301 ACALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVG 360
           A ALGPKDMD  GLSLFRPDF++ SFY+VFG +P+GFGCLL+KKSVIS L+  S  ++ G
Sbjct: 342 AGALGPKDMDSLGLSLFRPDFIITSFYRVFGYDPTGFGCLLIKKSVISCLQSQSGKTSSG 401

Query: 361 IVNLVP-------------------ADKLLQLAEDSSGTDTELEIQHRQFMSQMV----- 420
           IV + P                    D  + +  D+    T+L      + S  V     
Sbjct: 402 IVKITPEYPLYLSDSMDGLEGLTGIQDNGIAINGDNKALGTQLPAFSGAYTSAQVQDVFE 461

Query: 421 ----------------------------------------------------PSTSSFSG 480
                                                               P+ S  +G
Sbjct: 462 TDMDHEIGSDRDNTSAVFEEAESISVGELIKSPVFSEDESSDSSLWIDLGQSPADSDNAG 521

Query: 481 PIS------------------HPSMKTR-------------------------------- 540
            ++                   P+ K                                  
Sbjct: 522 HLNKQKSPLLVRKNHKRRSSPKPASKANNGSNGGRHVLSFDAAVLSVSHEVGEEVIEEEN 581

Query: 541 --------------TQFPEMEEEGN------------------------------LGSK- 600
                         T+  E EEEG                               LG + 
Sbjct: 582 SEMNQIDTSRRLRVTEIEEEEEEGGSSKLTAHANGSSSGIKDSAIRRETEGEFRLLGRRE 641

Query: 601 ----------VSEIEAVSK--------------------------------------QLE 605
                     V+E E  SK                                      + E
Sbjct: 642 KSQYNGGRLLVNEDEHPSKRRVSFRSVDHGEASVISLGDEDEEEDGSNGVEWDDDQREPE 701

BLAST of Spg034393 vs. TAIR 10
Match: AT4G37100.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 325.1 bits (832), Expect = 1.2e-88
Identity = 245/776 (31.57%), Postives = 353/776 (45.49%), Query Frame = 0

Query: 65  ETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLS-NHTCLDYIGIGLFSYHQFQ 124
           +++P   +AL+ FL+ +P+Y  +++IDQ+R+ +Y HL+ S +  CLDY G GLFSY Q  
Sbjct: 110 DSIPELREALTKFLSMYPKYQASEKIDQLRSDEYSHLSSSASKVCLDYCGFGLFSYVQTL 169

Query: 125 KHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALDS-DLESAVKRRIFRFLNVSESDYAM 184
            + +           F +S  + NL +  L    +S  +E  +K RI  +LN+ E++Y +
Sbjct: 170 HYWDTCT--------FSLSEITANLSNHALYGGAESGTVEHDIKTRIMDYLNIPENEYGL 229

Query: 185 VFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSWPRL 244
           VFT +R SAF+LLAESYPFQ++ +LLT++D+ES++V  M  T++ +GA   +A F WP L
Sbjct: 230 VFTVSRGSAFRLLAESYPFQSNKRLLTMFDHESQSVNWMAQTAREKGAKAYNAWFKWPTL 289

Query: 245 RINSRKLKEMI-VSKNKKKKAKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVDACA 304
           ++ S  LK+ +   K KKK +  GLFVFP  SR+TG +Y Y WM++AQ+N WHVL+DA +
Sbjct: 290 KLCSTDLKKRLSYKKRKKKDSAVGLFVFPAQSRVTGTKYSYQWMALAQQNHWHVLLDAGS 349

Query: 305 LGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVGIVN 364
           LGPKDMD  GLSLFRP+F++ SFY+VFG +P+GFGCLL+KKSV+  L+  S  +  GIV 
Sbjct: 350 LGPKDMDSLGLSLFRPEFIITSFYRVFGHDPTGFGCLLIKKSVMGSLQSQSGKTGSGIVK 409

Query: 365 LVPADKL--------------------------------------------------LQL 424
           + P   L                                                   +L
Sbjct: 410 ITPEYPLYLSDSVDGLDGLVGFEDHNDDKTKEAHRPGTQMPAFSGAYTSAQVRDVFETEL 469

Query: 425 AEDSSGTDTE------------------------------------LEI--------QHR 484
            ED+  +D +                                    +++        QH 
Sbjct: 470 LEDNISSDRDGTTSTTIFEETESVSVGELMKSPVFSEDESSDNSFWIDLGQSPLGSDQHN 529

Query: 485 QFMSQM-----------------VPSTSSFSGPI----------------------SHPS 544
           +  S +                  P   S+S P+                      S PS
Sbjct: 530 KIASPLPPIWLTNKRKQKQRQSPKPIPKSYSSPLYDGNDVLSFDAAVMSVTEHGTNSTPS 589

Query: 545 MKTRT-----QFPEMEEE------------GNLGSKVSE--------------------- 602
              R+        E++EE             N+ S++ E                     
Sbjct: 590 RNRRSSSNHLHVQEIQEENCGHSFANGLKSSNISSEIKESAIRRETEGEFRLLGGRDGGR 649

BLAST of Spg034393 vs. TAIR 10
Match: AT2G23520.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 322.0 bits (824), Expect = 1.0e-87
Identity = 245/782 (31.33%), Postives = 353/782 (45.14%), Query Frame = 0

Query: 61  FTNPETLPPPPQALSLFLAAFPQYHETQEIDQIRNKQYYHLNLSNHTCLDYIGIGLFSYH 120
           F + + +P   +A + FL  +P++  ++++DQ+R+ +Y HL L +  CLDY G GLFSY 
Sbjct: 104 FESEDDIPELLEAFNKFLTMYPKFETSEKVDQLRSDEYGHL-LDSKVCLDYCGFGLFSYV 163

Query: 121 QFQKHPNPFPSSNLNFPFFGVSYKSGNLKSRLLENALD-SDLESAVKRRIFRFLNVSESD 180
           Q   + +           F +S  + NL +  L    +   +E  +K RI  +LN+ ES+
Sbjct: 164 QTLHYWDSCT--------FSLSEITANLSNHALYGGAEIGTVEHDLKTRIMDYLNIPESE 223

Query: 181 YAMVFTANRTSAFKLLAESYPFQTSNKLLTVYDYESEAVEAMVSTSQNRGALTMSAEFSW 240
           Y +VFT +R SAF+LLAESYPF T+ +LLT++D+ES++V  M  T++ +GA   +A F W
Sbjct: 224 YGLVFTGSRGSAFRLLAESYPFHTNKRLLTMFDHESQSVNWMAQTAREKGAKAYNAWFKW 283

Query: 241 PRLRINSRKLKEMIVSKNKKKK-AKKGLFVFPLHSRITGARYPYLWMSVAQENRWHVLVD 300
           P L++ S  LK+ +  K +KKK +  GLFVFP  SR+TG++Y Y WM++AQ+N WHVL+D
Sbjct: 284 PTLKLCSTDLKKRLSHKKRKKKDSAVGLFVFPAQSRVTGSKYSYQWMALAQQNNWHVLLD 343

Query: 301 ACALGPKDMDCFGLSLFRPDFLVCSFYKVFGENPSGFGCLLVKKSVISILEGNSCSSNVG 360
           A +LGPKDMD  GLSLFRP+F++ SFYKVFG +P+GFGCLL+KKSV+  L+  S  +  G
Sbjct: 344 AGSLGPKDMDSLGLSLFRPEFIITSFYKVFGHDPTGFGCLLIKKSVMGNLQSQSGKTGSG 403

Query: 361 IVNLVPADKL-------------------------------------------------- 420
           IV + P   L                                                  
Sbjct: 404 IVKITPQYPLYLSDSIDGLDGLVGLEDHDIGTNGDKPATTDAARRGAQMPVFSGAYTSAQ 463

Query: 421 -----------------------------------------------------LQLAEDS 480
                                                                + L +  
Sbjct: 464 VRDVFETDLLEDNASDRDGTSSTIFEENESVSVGELMKSPAFSEDESSDNSFWIDLGQSP 523

Query: 481 SGTDTELEIQHRQFMSQM------------VPSTSSFSGPI------------------- 540
            G+D+   + H +  S +             P   S+S P+                   
Sbjct: 524 LGSDSAGHLNHHKIASPLPPFWFTSKRQSPKPVAKSYSSPMYDGKDVLSFDAAVMSVTQE 583

Query: 541 --SHPSMKTRT----QFPEMEEE--GNL-------------------------------- 600
             S PS   R     Q  E++EE  GN+                                
Sbjct: 584 INSTPSRNLRNSNNLQIQEIQEENCGNIVYRAGSGFGSNGSSSKISSDMKDNAIRRETEG 643

Query: 601 ------------------------GSKVS-EIEAVSKQL--------------------- 602
                                   G++VS  ++ VS  L                     
Sbjct: 644 EFRLLGRRGTGGRLLGLEDEQPSRGTRVSFNMDRVSHSLDQGEASLASVYDESDGENPNE 703