Spg031423 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031423
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPhosphoenolpyruvate carboxylase kinase 1-like
Locationscaffold11: 40505234 .. 40506064 (-)
RNA-Seq ExpressionSpg031423
SyntenySpg031423
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGATTCATTCAAGAGAGACTATCAAGTAATCGAAGAAATCGGCCGGGGAAGATTCGGCACCGTTTTCCGATGCACTTCCAGATCCTCCGCTGGATTCTACGCCGTTAAAACGATCGACAAGCGCCGGATCTCCGCAGGTGATTCACTCGACGCCGAATGCCTCGTCAACGAGACGAAGATTCTTCATCTCCTTTACCCTCATCCTCACATTCTTGCGCTGCATAATCTCTACGAAGATGAATCTCACCTCCATATGGTTCTCGATCTCTGTAGCTCTTCCGATCTTCATCGGCGAGTCACTCTCCAGGTCTTTTCCGAGGCGGAGGCCGCTTTGGTTATGTCGCAACTGATGAACGCGGTGGCTCATTGTCACCGTCTCGGCGTTGCGCACCGCGATATTAAACCAGACAACATTCTGTTCGACGAGTGGAATTCGGTGAAACTGGCGGATTTTGGATCGGCCGAGATGTTTAATAAGGGAGAAGAGTCGATGAGTGGCGTCGTTGGAACGCCGTACTACGTGGCGCCGGAAGTGCTGGCTGGAAGAGATTACGGCGAGAAGGTCGACGTGTGGAGCGCCGGCGTCGTTTTGTACGTGATGTTGGCTGGTTTTCCACCGTTTCACGGAGAATCGGTAGTTGAGATCTTTCACGCGGTGTTGCGAGCGAATTTGAGATTTCCGTCCAGAGTTTTCCACTCTGTATCTCCCGCGGCGAAAGACTTGCTCCGTAAAATGCTCTGTAAAGACGTTTCCAGAAGATTCTCCGCCGAACAAGTCCTCAGTAAGTATCTAATTTACGTTTACAATCGATCAATATTTTCCTGA

mRNA sequence

ATGAGCGATTCATTCAAGAGAGACTATCAAGTAATCGAAGAAATCGGCCGGGGAAGATTCGGCACCGTTTTCCGATGCACTTCCAGATCCTCCGCTGGATTCTACGCCGTTAAAACGATCGACAAGCGCCGGATCTCCGCAGGTGATTCACTCGACGCCGAATGCCTCGTCAACGAGACGAAGATTCTTCATCTCCTTTACCCTCATCCTCACATTCTTGCGCTGCATAATCTCTACGAAGATGAATCTCACCTCCATATGGTTCTCGATCTCTGTAGCTCTTCCGATCTTCATCGGCGAGTCACTCTCCAGGTCTTTTCCGAGGCGGAGGCCGCTTTGGTTATGTCGCAACTGATGAACGCGGTGGCTCATTGTCACCGTCTCGGCGTTGCGCACCGCGATATTAAACCAGACAACATTCTGTTCGACGAGTGGAATTCGGTGAAACTGGCGGATTTTGGATCGGCCGAGATGTTTAATAAGGGAGAAGAGTCGATGAGTGGCGTCGTTGGAACGCCGTACTACGTGGCGCCGGAAGTGCTGGCTGGAAGAGATTACGGCGAGAAGGTCGACGTGTGGAGCGCCGGCGTCGTTTTGTACGTGATGTTGGCTGGTTTTCCACCGTTTCACGGAGAATCGGTAGTTGAGATCTTTCACGCGGTGTTGCGAGCGAATTTGAGATTTCCGTCCAGAGTTTTCCACTCTGTATCTCCCGCGGCGAAAGACTTGCTCCGTAAAATGCTCTGTAAAGACGTTTCCAGAAGATTCTCCGCCGAACAAGTCCTCAGTAAGTATCTAATTTACGTTTACAATCGATCAATATTTTCCTGA

Coding sequence (CDS)

ATGAGCGATTCATTCAAGAGAGACTATCAAGTAATCGAAGAAATCGGCCGGGGAAGATTCGGCACCGTTTTCCGATGCACTTCCAGATCCTCCGCTGGATTCTACGCCGTTAAAACGATCGACAAGCGCCGGATCTCCGCAGGTGATTCACTCGACGCCGAATGCCTCGTCAACGAGACGAAGATTCTTCATCTCCTTTACCCTCATCCTCACATTCTTGCGCTGCATAATCTCTACGAAGATGAATCTCACCTCCATATGGTTCTCGATCTCTGTAGCTCTTCCGATCTTCATCGGCGAGTCACTCTCCAGGTCTTTTCCGAGGCGGAGGCCGCTTTGGTTATGTCGCAACTGATGAACGCGGTGGCTCATTGTCACCGTCTCGGCGTTGCGCACCGCGATATTAAACCAGACAACATTCTGTTCGACGAGTGGAATTCGGTGAAACTGGCGGATTTTGGATCGGCCGAGATGTTTAATAAGGGAGAAGAGTCGATGAGTGGCGTCGTTGGAACGCCGTACTACGTGGCGCCGGAAGTGCTGGCTGGAAGAGATTACGGCGAGAAGGTCGACGTGTGGAGCGCCGGCGTCGTTTTGTACGTGATGTTGGCTGGTTTTCCACCGTTTCACGGAGAATCGGTAGTTGAGATCTTTCACGCGGTGTTGCGAGCGAATTTGAGATTTCCGTCCAGAGTTTTCCACTCTGTATCTCCCGCGGCGAAAGACTTGCTCCGTAAAATGCTCTGTAAAGACGTTTCCAGAAGATTCTCCGCCGAACAAGTCCTCAGTAAGTATCTAATTTACGTTTACAATCGATCAATATTTTCCTGA

Protein sequence

MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETKILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMNAVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEVLAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAAKDLLRKMLCKDVSRRFSAEQVLSKYLIYVYNRSIFS
Homology
BLAST of Spg031423 vs. NCBI nr
Match: KAA0036146.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 501.5 bits (1290), Expect = 4.6e-138
Identity = 245/266 (92.11%), Postives = 256/266 (96.24%), Query Frame = 0

Query: 1   MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNET 60
           MSD FKRDYQVIEE+GRGRFG VFRC SRSS G YAVK IDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMN 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRR+TLQ+FSEAEAA +MSQLM+
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEV 180
           AVAHCHR GVAHRDIKPDNILFDEW+SVKLADFGSAEMF +GEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240
           LAG+DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP+A
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRFSAEQVLSKYL 267
           KDLLRKMLCKDVSRR SAEQVLSKY+
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVLSKYI 266

BLAST of Spg031423 vs. NCBI nr
Match: XP_038900016.1 (phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida])

HSP 1 Score: 495.0 bits (1273), Expect = 4.3e-136
Identity = 243/262 (92.75%), Postives = 250/262 (95.42%), Query Frame = 0

Query: 1   MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNET 60
           MSD FKRDYQVIEEIGRGRFGTVFRCTSRSS G YAVK IDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEEIGRGRFGTVFRCTSRSSGGIYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMN 120
           KI+HLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRR+TL+VFSE EAA +MSQLM+
Sbjct: 61  KIIHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLRVFSEVEAATIMSQLMH 120

Query: 121 AVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEV 180
           AVAHCHR GVAHRDIKPDNILFDEW+SVKLADFGSAEMF  GEESMSGVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKHGEESMSGVVGTPYYVAPEV 180

Query: 181 LAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240
           LAG+ YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP A
Sbjct: 181 LAGKKYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPPA 240

Query: 241 KDLLRKMLCKDVSRRFSAEQVL 263
           KDLLRKMLCKDVSRR SAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

BLAST of Spg031423 vs. NCBI nr
Match: XP_008438527.1 (PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo] >TYK17304.1 phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 494.6 bits (1272), Expect = 5.6e-136
Identity = 242/262 (92.37%), Postives = 252/262 (96.18%), Query Frame = 0

Query: 1   MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNET 60
           MSD FKRDYQVIEE+GRGRFG VFRC SRSS G YAVK IDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMN 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRR+TLQ+FSEAEAA +MSQLM+
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEV 180
           AVAHCHR GVAHRDIKPDNILFDEW+SVKLADFGSAEMF +GEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240
           LAG+DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP+A
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRFSAEQVL 263
           KDLLRKMLCKDVSRR SAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

BLAST of Spg031423 vs. NCBI nr
Match: XP_031739464.1 (phosphoenolpyruvate carboxylase kinase 1 [Cucumis sativus] >KGN56859.1 hypothetical protein Csa_009620 [Cucumis sativus])

HSP 1 Score: 493.8 bits (1270), Expect = 9.6e-136
Identity = 242/262 (92.37%), Postives = 251/262 (95.80%), Query Frame = 0

Query: 1   MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNET 60
           MSD FKRDYQVIEE+GRGRFGTVFRC S+S  G YAVK IDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMN 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRR+TLQVFSEAEAA +MSQLM+
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEV 180
           AVAHCHR GVAHRDIKPDNILFDEW+SVKLADFGS EMF +GEESMSGVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180

Query: 181 LAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240
           LAG+DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP+A
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRFSAEQVL 263
           KDLLRKMLCKDVSRR SAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

BLAST of Spg031423 vs. NCBI nr
Match: XP_022924623.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata])

HSP 1 Score: 493.4 bits (1269), Expect = 1.2e-135
Identity = 244/262 (93.13%), Postives = 250/262 (95.42%), Query Frame = 0

Query: 1   MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNET 60
           MSD  KRDYQVIEEIGRGRFGTVFRCTSRSS   YAVKTIDKRRI AGDSLDAECLV ET
Sbjct: 1   MSDDLKRDYQVIEEIGRGRFGTVFRCTSRSSGENYAVKTIDKRRILAGDSLDAECLVKET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMN 120
           KILHLLYPHPHILALHNLYEDE HLHMVLDLCSSSDLHRRVTLQVFSEAEAA++MSQLM+
Sbjct: 61  KILHLLYPHPHILALHNLYEDEFHLHMVLDLCSSSDLHRRVTLQVFSEAEAAMIMSQLMH 120

Query: 121 AVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEV 180
           AVAHCHR GVAHRDIKPDNILFDEW+SVKLADFGSAEMF +GEESM GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRYGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMRGVVGTPYYVAPEV 180

Query: 181 LAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240
           LAG+DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240

Query: 241 KDLLRKMLCKDVSRRFSAEQVL 263
           KDLLRKMLCKDVSRR SAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

BLAST of Spg031423 vs. ExPASy Swiss-Prot
Match: Q9SPK4 (Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK1 PE=1 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.6e-72
Identity = 145/257 (56.42%), Postives = 181/257 (70.43%), Query Frame = 0

Query: 9   YQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETKILHLLYP 68
           YQ+ EEIGRGRFGTV R  + ++  F+A KTIDK  +S  D LD  CL NE K++ LL  
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLS--DDLDRACLDNEPKLMALLSY 74

Query: 69  HPHILALHNLYEDESHLHMVLDLC--SSSDLHRRVTLQVFSEAEAALVMSQLMNAVAHCH 128
           HP+I+ +H+L + +S L + ++L   S S   R V+   F E + A    Q++ A++HCH
Sbjct: 75  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 134

Query: 129 RLGVAHRDIKPDNILFDEWN-SVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEVLAGRD 188
           R GV HRDIKP+NIL D  N +VK+ DFGS     +G E+  GVVGTPYYVAPEVL G  
Sbjct: 135 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEG-ETTEGVVGTPYYVAPEVLMGYS 194

Query: 189 YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAAKDLLR 248
           YGEKVD+WSAGVVLY MLAG PPF+GE+  EIF AVLR NLRFP+++F  VS  AKD LR
Sbjct: 195 YGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLR 254

Query: 249 KMLCKDVSRRFSAEQVL 263
           K++CKD SRRFSAEQ L
Sbjct: 255 KLICKDASRRFSAEQAL 268

BLAST of Spg031423 vs. ExPASy Swiss-Prot
Match: Q93VK0 (Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK2 PE=1 SV=2)

HSP 1 Score: 270.4 bits (690), Expect = 2.3e-71
Identity = 142/261 (54.41%), Postives = 185/261 (70.88%), Query Frame = 0

Query: 6   KRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETKILHL 65
           + +YQ+ +EIGRGRFGT+ RC S ++  FYA KTIDKR +   D+LD EC+  E +I+ +
Sbjct: 8   ENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLI--DALDRECIETEPRIMAM 67

Query: 66  LYPHPHILALHNLYEDESHLHMVLDLCS-SSDLHRRVTLQ--VFSEAEAALVMSQLMNAV 125
           L PHP+I+ + +LYE E  L +V++L      ++ R+       SE+E+A    Q+++A+
Sbjct: 68  LPPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSAL 127

Query: 126 AHCHRLGVAHRDIKPDNILFD-EWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEVL 185
           AHCHR  V HRD+KPDN+L D     VKL DFGSA     G E+  GVVGTPYYVAPEV+
Sbjct: 128 AHCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWL--GGETAEGVVGTPYYVAPEVV 187

Query: 186 AGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAAK 245
            GR Y EKVD+WSAGVV+Y MLAG PPF+GE+  +IF ++LR NLRFP + F SVS  AK
Sbjct: 188 MGRKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAK 247

Query: 246 DLLRKMLCKDVSRRFSAEQVL 263
           DLLRKM+C+DVSRRFSAE  L
Sbjct: 248 DLLRKMICRDVSRRFSAEDAL 264

BLAST of Spg031423 vs. ExPASy Swiss-Prot
Match: Q38870 (Calcium-dependent protein kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CPK2 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 8.1e-53
Identity = 112/265 (42.26%), Postives = 170/265 (64.15%), Query Frame = 0

Query: 2   SDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETK 61
           +++FK  Y +  ++G+G+FGT F C  + +   YA K+I KR++   +  D E +  E +
Sbjct: 179 TENFKEFYSLGRKLGQGQFGTTFLCLEKGTGNEYACKSISKRKLLTDE--DVEDVRREIQ 238

Query: 62  ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQ-VFSEAEAALVMSQLMN 121
           I+H L  HP+++++   YED   +H+V++LCS  +L  R+  +  ++E +AA +   ++ 
Sbjct: 239 IMHHLAGHPNVISIKGAYEDVVAVHLVMELCSGGELFDRIIQRGHYTERKAAELARTIVG 298

Query: 122 AVAHCHRLGVAHRDIKPDNILF---DEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVA 181
            +  CH LGV HRD+KP+N LF   +E + +K  DFG   MF K +E  + VVG+PYYVA
Sbjct: 299 VLEACHSLGVMHRDLKPENFLFVSREEDSLLKTIDFG-LSMFFKPDEVFTDVVGSPYYVA 358

Query: 182 PEVLAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVS 241
           PEVL  R YG + DVWSAGV++Y++L+G PPF  E+   IF  VL  +L F S  + S+S
Sbjct: 359 PEVLRKR-YGPESDVWSAGVIVYILLSGVPPFWAETEQGIFEQVLHGDLDFSSDPWPSIS 418

Query: 242 PAAKDLLRKMLCKDVSRRFSAEQVL 263
            +AKDL+RKML +D  RR +A QVL
Sbjct: 419 ESAKDLVRKMLVRDPKRRLTAHQVL 439

BLAST of Spg031423 vs. ExPASy Swiss-Prot
Match: P28583 (Calcium-dependent protein kinase SK5 OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 4.0e-52
Identity = 112/265 (42.26%), Postives = 167/265 (63.02%), Query Frame = 0

Query: 2   SDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETK 61
           + + +  Y+V  ++G+G+FGT F CT R+S G +A K+I KR++   +  D E +  E +
Sbjct: 27  TQNIREVYEVGRKLGQGQFGTTFECTRRASGGKFACKSIPKRKLLCKE--DYEDVWREIQ 86

Query: 62  ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQ-VFSEAEAALVMSQLMN 121
           I+H L  H +++ +   YED + +H+V++LC   +L  R+  +  +SE +AA ++  ++ 
Sbjct: 87  IMHHLSEHANVVRIEGTYEDSTAVHLVMELCEGGELFDRIVQKGHYSERQAARLIKTIVE 146

Query: 122 AVAHCHRLGVAHRDIKPDNILF---DEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVA 181
            V  CH LGV HRD+KP+N LF   DE   +K  DFG   +F K  ES   VVG+PYYVA
Sbjct: 147 VVEACHSLGVMHRDLKPENFLFDTIDEDAKLKATDFG-LSVFYKPGESFCDVVGSPYYVA 206

Query: 182 PEVLAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVS 241
           PEVL  + YG + DVWSAGV+LY++L+G PPF  ES   IF  +L   L F S  + S+S
Sbjct: 207 PEVLR-KLYGPESDVWSAGVILYILLSGVPPFWAESEPGIFRQILLGKLDFHSEPWPSIS 266

Query: 242 PAAKDLLRKMLCKDVSRRFSAEQVL 263
            +AKDL+RKML ++   R +A +VL
Sbjct: 267 DSAKDLIRKMLDQNPKTRLTAHEVL 287

BLAST of Spg031423 vs. ExPASy Swiss-Prot
Match: Q53P85 (Calcium-dependent protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK24 PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 6.8e-52
Identity = 108/258 (41.86%), Postives = 165/258 (63.95%), Query Frame = 0

Query: 9   YQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETKILHLLYP 68
           Y++ +++G+G+FGT + C  ++S G +A K+I KR++   +  D E +  E +I+H L  
Sbjct: 47  YRIGKKLGQGQFGTTYLCVDKASGGEFACKSIPKRKLLCRE--DYEDVWREIQIMHHLSE 106

Query: 69  HPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQ-VFSEAEAALVMSQLMNAVAHCHR 128
           HP+++ +   YED   +H+V++LC+  +L  R+  +  ++E  AA ++  ++  V  CH 
Sbjct: 107 HPNVVRIRGAYEDALFVHIVMELCAGGELFDRIVAKGHYTERAAAQLIRTIVAVVEGCHS 166

Query: 129 LGVAHRDIKPDNILF---DEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEVLAGR 188
           LGV HRD+KP+N LF    E   +K  DFG   MF K  +  S VVG+PYYVAPEVL  +
Sbjct: 167 LGVMHRDLKPENFLFASAAEDAPLKATDFG-LSMFYKPGDKFSDVVGSPYYVAPEVLQ-K 226

Query: 189 DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAAKDLL 248
            YG + DVWSAGV+LY++L G PPF  E+   IF  +LR  L F S  + S+S +AKDL+
Sbjct: 227 CYGPESDVWSAGVILYILLCGVPPFWAETEAGIFRQILRGKLDFESEPWPSISDSAKDLV 286

Query: 249 RKMLCKDVSRRFSAEQVL 263
           R MLC+D ++R +A +VL
Sbjct: 287 RNMLCRDPTKRLTAHEVL 300

BLAST of Spg031423 vs. ExPASy TrEMBL
Match: A0A5A7T1U4 (Phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1463G00010 PE=3 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.2e-138
Identity = 245/266 (92.11%), Postives = 256/266 (96.24%), Query Frame = 0

Query: 1   MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNET 60
           MSD FKRDYQVIEE+GRGRFG VFRC SRSS G YAVK IDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMN 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRR+TLQ+FSEAEAA +MSQLM+
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEV 180
           AVAHCHR GVAHRDIKPDNILFDEW+SVKLADFGSAEMF +GEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240
           LAG+DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP+A
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRFSAEQVLSKYL 267
           KDLLRKMLCKDVSRR SAEQVLSKY+
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVLSKYI 266

BLAST of Spg031423 vs. ExPASy TrEMBL
Match: A0A5D3D228 (Phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001580 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 2.7e-136
Identity = 242/262 (92.37%), Postives = 252/262 (96.18%), Query Frame = 0

Query: 1   MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNET 60
           MSD FKRDYQVIEE+GRGRFG VFRC SRSS G YAVK IDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMN 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRR+TLQ+FSEAEAA +MSQLM+
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEV 180
           AVAHCHR GVAHRDIKPDNILFDEW+SVKLADFGSAEMF +GEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240
           LAG+DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP+A
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRFSAEQVL 263
           KDLLRKMLCKDVSRR SAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

BLAST of Spg031423 vs. ExPASy TrEMBL
Match: A0A1S3AWP8 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo OX=3656 GN=LOC103483595 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 2.7e-136
Identity = 242/262 (92.37%), Postives = 252/262 (96.18%), Query Frame = 0

Query: 1   MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNET 60
           MSD FKRDYQVIEE+GRGRFG VFRC SRSS G YAVK IDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMN 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRR+TLQ+FSEAEAA +MSQLM+
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEV 180
           AVAHCHR GVAHRDIKPDNILFDEW+SVKLADFGSAEMF +GEESM+GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYYVAPEV 180

Query: 181 LAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240
           LAG+DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP+A
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRFSAEQVL 263
           KDLLRKMLCKDVSRR SAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

BLAST of Spg031423 vs. ExPASy TrEMBL
Match: A0A0A0L743 (Phosphoenolpyruvate carboxylase kinase 4 OS=Cucumis sativus OX=3659 GN=Csa_3G135070 PE=3 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 4.6e-136
Identity = 242/262 (92.37%), Postives = 251/262 (95.80%), Query Frame = 0

Query: 1   MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNET 60
           MSD FKRDYQVIEE+GRGRFGTVFRC S+S  G YAVK IDKRRISAGDSLDAECLVNET
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGTVFRCISKSCGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMN 120
           KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRR+TLQVFSEAEAA +MSQLM+
Sbjct: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQVFSEAEAARIMSQLMH 120

Query: 121 AVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEV 180
           AVAHCHR GVAHRDIKPDNILFDEW+SVKLADFGS EMF +GEESMSGVVGTPYYVAPEV
Sbjct: 121 AVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSTEMFKQGEESMSGVVGTPYYVAPEV 180

Query: 181 LAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240
           LAG+DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSP+A
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPSA 240

Query: 241 KDLLRKMLCKDVSRRFSAEQVL 263
           KDLLRKMLCKDVSRR SAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

BLAST of Spg031423 vs. ExPASy TrEMBL
Match: A0A6J1ED04 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111432056 PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 6.0e-136
Identity = 244/262 (93.13%), Postives = 250/262 (95.42%), Query Frame = 0

Query: 1   MSDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNET 60
           MSD  KRDYQVIEEIGRGRFGTVFRCTSRSS   YAVKTIDKRRI AGDSLDAECLV ET
Sbjct: 1   MSDDLKRDYQVIEEIGRGRFGTVFRCTSRSSGENYAVKTIDKRRILAGDSLDAECLVKET 60

Query: 61  KILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQVFSEAEAALVMSQLMN 120
           KILHLLYPHPHILALHNLYEDE HLHMVLDLCSSSDLHRRVTLQVFSEAEAA++MSQLM+
Sbjct: 61  KILHLLYPHPHILALHNLYEDEFHLHMVLDLCSSSDLHRRVTLQVFSEAEAAMIMSQLMH 120

Query: 121 AVAHCHRLGVAHRDIKPDNILFDEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEV 180
           AVAHCHR GVAHRDIKPDNILFDEW+SVKLADFGSAEMF +GEESM GVVGTPYYVAPEV
Sbjct: 121 AVAHCHRYGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMRGVVGTPYYVAPEV 180

Query: 181 LAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240
           LAG+DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA
Sbjct: 181 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAA 240

Query: 241 KDLLRKMLCKDVSRRFSAEQVL 263
           KDLLRKMLCKDVSRR SAEQVL
Sbjct: 241 KDLLRKMLCKDVSRRISAEQVL 262

BLAST of Spg031423 vs. TAIR 10
Match: AT1G08650.2 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 275.8 bits (704), Expect = 3.8e-74
Identity = 146/258 (56.59%), Postives = 182/258 (70.54%), Query Frame = 0

Query: 9   YQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETKILHLLYP 68
           YQ+ EEIGRGRFGTV R  + ++  F+A KTIDK  +S  D LD  CL NE K++ LL  
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLS--DDLDRACLDNEPKLMALLSY 74

Query: 69  HPHILALHNLYEDESHLHMVLDLC--SSSDLHRRVTLQVFSEAEAALVMSQLMNAVAHCH 128
           HP+I+ +H+L + +S L + ++L   S S   R V+   F E + A    Q++ A++HCH
Sbjct: 75  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 134

Query: 129 RLGVAHRDIKPDNILFDEWN-SVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEVLAGRD 188
           R GV HRDIKP+NIL D  N +VK+ DFGS     +G E+  GVVGTPYYVAPEVL G  
Sbjct: 135 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEG-ETTEGVVGTPYYVAPEVLMGYS 194

Query: 189 YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAAKDLLR 248
           YGEKVD+WSAGVVLY MLAG PPF+GE+  EIF AVLR NLRFP+++F  VS  AKD LR
Sbjct: 195 YGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLR 254

Query: 249 KMLCKDVSRRFSAEQVLS 264
           K++CKD SRRFSAEQ LS
Sbjct: 255 KLICKDASRRFSAEQALS 269

BLAST of Spg031423 vs. TAIR 10
Match: AT1G08650.1 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 274.2 bits (700), Expect = 1.1e-73
Identity = 145/257 (56.42%), Postives = 181/257 (70.43%), Query Frame = 0

Query: 9   YQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETKILHLLYP 68
           YQ+ EEIGRGRFGTV R  + ++  F+A KTIDK  +S  D LD  CL NE K++ LL  
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLS--DDLDRACLDNEPKLMALLSY 74

Query: 69  HPHILALHNLYEDESHLHMVLDLC--SSSDLHRRVTLQVFSEAEAALVMSQLMNAVAHCH 128
           HP+I+ +H+L + +S L + ++L   S S   R V+   F E + A    Q++ A++HCH
Sbjct: 75  HPNIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCH 134

Query: 129 RLGVAHRDIKPDNILFDEWN-SVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEVLAGRD 188
           R GV HRDIKP+NIL D  N +VK+ DFGS     +G E+  GVVGTPYYVAPEVL G  
Sbjct: 135 RYGVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEG-ETTEGVVGTPYYVAPEVLMGYS 194

Query: 189 YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAAKDLLR 248
           YGEKVD+WSAGVVLY MLAG PPF+GE+  EIF AVLR NLRFP+++F  VS  AKD LR
Sbjct: 195 YGEKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLR 254

Query: 249 KMLCKDVSRRFSAEQVL 263
           K++CKD SRRFSAEQ L
Sbjct: 255 KLICKDASRRFSAEQAL 268

BLAST of Spg031423 vs. TAIR 10
Match: AT3G04530.1 (phosphoenolpyruvate carboxylase kinase 2 )

HSP 1 Score: 270.4 bits (690), Expect = 1.6e-72
Identity = 142/261 (54.41%), Postives = 185/261 (70.88%), Query Frame = 0

Query: 6   KRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETKILHL 65
           + +YQ+ +EIGRGRFGT+ RC S ++  FYA KTIDKR +   D+LD EC+  E +I+ +
Sbjct: 8   ENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVLI--DALDRECIETEPRIMAM 67

Query: 66  LYPHPHILALHNLYEDESHLHMVLDLCS-SSDLHRRVTLQ--VFSEAEAALVMSQLMNAV 125
           L PHP+I+ + +LYE E  L +V++L      ++ R+       SE+E+A    Q+++A+
Sbjct: 68  LPPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSAL 127

Query: 126 AHCHRLGVAHRDIKPDNILFD-EWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEVL 185
           AHCHR  V HRD+KPDN+L D     VKL DFGSA     G E+  GVVGTPYYVAPEV+
Sbjct: 128 AHCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWL--GGETAEGVVGTPYYVAPEVV 187

Query: 186 AGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAAK 245
            GR Y EKVD+WSAGVV+Y MLAG PPF+GE+  +IF ++LR NLRFP + F SVS  AK
Sbjct: 188 MGRKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEAK 247

Query: 246 DLLRKMLCKDVSRRFSAEQVL 263
           DLLRKM+C+DVSRRFSAE  L
Sbjct: 248 DLLRKMICRDVSRRFSAEDAL 264

BLAST of Spg031423 vs. TAIR 10
Match: AT3G10660.1 (calmodulin-domain protein kinase cdpk isoform 2 )

HSP 1 Score: 208.8 bits (530), Expect = 5.7e-54
Identity = 112/265 (42.26%), Postives = 170/265 (64.15%), Query Frame = 0

Query: 2   SDSFKRDYQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETK 61
           +++FK  Y +  ++G+G+FGT F C  + +   YA K+I KR++   +  D E +  E +
Sbjct: 179 TENFKEFYSLGRKLGQGQFGTTFLCLEKGTGNEYACKSISKRKLLTDE--DVEDVRREIQ 238

Query: 62  ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQ-VFSEAEAALVMSQLMN 121
           I+H L  HP+++++   YED   +H+V++LCS  +L  R+  +  ++E +AA +   ++ 
Sbjct: 239 IMHHLAGHPNVISIKGAYEDVVAVHLVMELCSGGELFDRIIQRGHYTERKAAELARTIVG 298

Query: 122 AVAHCHRLGVAHRDIKPDNILF---DEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVA 181
            +  CH LGV HRD+KP+N LF   +E + +K  DFG   MF K +E  + VVG+PYYVA
Sbjct: 299 VLEACHSLGVMHRDLKPENFLFVSREEDSLLKTIDFG-LSMFFKPDEVFTDVVGSPYYVA 358

Query: 182 PEVLAGRDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVS 241
           PEVL  R YG + DVWSAGV++Y++L+G PPF  E+   IF  VL  +L F S  + S+S
Sbjct: 359 PEVLRKR-YGPESDVWSAGVIVYILLSGVPPFWAETEQGIFEQVLHGDLDFSSDPWPSIS 418

Query: 242 PAAKDLLRKMLCKDVSRRFSAEQVL 263
            +AKDL+RKML +D  RR +A QVL
Sbjct: 419 ESAKDLVRKMLVRDPKRRLTAHQVL 439

BLAST of Spg031423 vs. TAIR 10
Match: AT1G76040.2 (calcium-dependent protein kinase 29 )

HSP 1 Score: 201.8 bits (512), Expect = 7.0e-52
Identity = 107/258 (41.47%), Postives = 164/258 (63.57%), Query Frame = 0

Query: 9   YQVIEEIGRGRFGTVFRCTSRSSAGFYAVKTIDKRRISAGDSLDAECLVNETKILHLLYP 68
           Y + +E+GRG+FG  ++CT +S+   YA K+I KR++      D E +  E  IL  L  
Sbjct: 112 YDLHKELGRGQFGITYKCTDKSNGREYACKSISKRKLIR--RKDIEDVRREVMILQHLTG 171

Query: 69  HPHILALHNLYEDESHLHMVLDLCSSSDLHRRVTLQ-VFSEAEAALVMSQLMNAVAHCHR 128
            P+I+     YED+ +LH+V++LCS  +L  R+  +  +SE EAA +  Q++N V  CH 
Sbjct: 172 QPNIVEFRGAYEDKDNLHLVMELCSGGELFDRIIKKGSYSEKEAANIFRQIVNVVHVCHF 231

Query: 129 LGVAHRDIKPDNILF---DEWNSVKLADFGSAEMFNKGEESMSGVVGTPYYVAPEVLAGR 188
           +GV HRD+KP+N L    +E + +K  DFG +    +G +    +VG+ YYVAPEVL  R
Sbjct: 232 MGVVHRDLKPENFLLVSNEEDSPIKATDFGLSVFIEEG-KVYRDIVGSAYYVAPEVL-HR 291

Query: 189 DYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHSVSPAAKDLL 248
           +YG+++DVWSAGV+LY++L+G PPF GE+   IF A+L   L   +  + ++S +AKDL+
Sbjct: 292 NYGKEIDVWSAGVMLYILLSGVPPFWGETEKTIFEAILEGKLDLETSPWPTISESAKDLI 351

Query: 249 RKMLCKDVSRRFSAEQVL 263
           RKML +D  +R +A + L
Sbjct: 352 RKMLIRDPKKRITAAEAL 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0036146.14.6e-13892.11phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo var. makuwa][more]
XP_038900016.14.3e-13692.75phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida][more]
XP_008438527.15.6e-13692.37PREDICTED: phosphoenolpyruvate carboxylase kinase 1-like [Cucumis melo] >TYK1730... [more]
XP_031739464.19.6e-13692.37phosphoenolpyruvate carboxylase kinase 1 [Cucumis sativus] >KGN56859.1 hypotheti... [more]
XP_022924623.11.2e-13593.13phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SPK41.6e-7256.42Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q93VK02.3e-7154.41Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q388708.1e-5342.26Calcium-dependent protein kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CPK2 PE=1 ... [more]
P285834.0e-5242.26Calcium-dependent protein kinase SK5 OS=Glycine max OX=3847 PE=1 SV=1[more]
Q53P856.8e-5241.86Calcium-dependent protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
A0A5A7T1U42.2e-13892.11Phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3D2282.7e-13692.37Phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3AWP82.7e-13692.37phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A0A0L7434.6e-13692.37Phosphoenolpyruvate carboxylase kinase 4 OS=Cucumis sativus OX=3659 GN=Csa_3G135... [more]
A0A6J1ED046.0e-13693.13phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G08650.23.8e-7456.59phosphoenolpyruvate carboxylase kinase 1 [more]
AT1G08650.11.1e-7356.42phosphoenolpyruvate carboxylase kinase 1 [more]
AT3G04530.11.6e-7254.41phosphoenolpyruvate carboxylase kinase 2 [more]
AT3G10660.15.7e-5442.26calmodulin-domain protein kinase cdpk isoform 2 [more]
AT1G76040.27.0e-5241.47calcium-dependent protein kinase 29 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 9..266
e-value: 3.7E-90
score: 315.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 9..264
e-value: 5.8E-71
score: 238.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 9..269
score: 48.498714
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 2..273
e-value: 9.9E-84
score: 283.3
NoneNo IPR availablePIRSFPIRSF000654ILKcoord: 3..269
e-value: 5.4E-23
score: 79.3
NoneNo IPR availablePIRSRPIRSR000550-2PIRSR000550-2coord: 7..260
e-value: 2.9E-48
score: 162.6
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 3..266
NoneNo IPR availablePANTHERPTHR24349:SF410PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE 1coord: 3..266
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 8..263
e-value: 3.32814E-115
score: 329.822
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 15..38
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 130..142
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 6..263

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031423.1Spg031423.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity