Spg025189 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025189
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptioncalmodulin-like protein 12
Locationscaffold13: 39442528 .. 39443055 (-)
RNA-Seq ExpressionSpg025189
SyntenySpg025189
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGTCTCCCAACTTCAAGCATGGGCTGAGGATGAAGCAAATGAATTGATCAAGAAGTATGATAAAAATGGTGATGGTGTTCTCACTAAAGATGAGCTACAATCCTTCCTCCACGATGTTCGAAGTTCAAATCAACTCAACAATCAAGGGTTCATATCTAAAATCCCAGAAATGAATGATGGTATTATGCACAAGCTTAGTTCAAACACTCATGTACAACCAAAAAAGCGATTACAAGACAAAGCTCAAAAATTCTCAGCTAAAGTTCTTGATAGAAAGGTATCTAATATGTCACTTAGTCAAGAGCAGATGAAGGAGATTTTCAAACATCATGACAACAACAAAGATGGTTTTCTCAGCACAAGGGAGCTAACAAAAGCTTTTGGTTTTCTAGGCTCAATTCTTCCATTCTACAAAGCTCACTATGGTTTGGCTTATGCAGATGCTGATAATGATGGTCTTATTAGTGAGGCTGAGCTTGACAAACTCGTTGATTATGCTAGTAAAGTCATGAAGAAGAAATGA

mRNA sequence

ATGTCTGTCTCCCAACTTCAAGCATGGGCTGAGGATGAAGCAAATGAATTGATCAAGAAGTATGATAAAAATGGTGATGGTGTTCTCACTAAAGATGAGCTACAATCCTTCCTCCACGATGTTCGAAGTTCAAATCAACTCAACAATCAAGGGTTCATATCTAAAATCCCAGAAATGAATGATGGTATTATGCACAAGCTTAGTTCAAACACTCATGTACAACCAAAAAAGCGATTACAAGACAAAGCTCAAAAATTCTCAGCTAAAGTTCTTGATAGAAAGGTATCTAATATGTCACTTAGTCAAGAGCAGATGAAGGAGATTTTCAAACATCATGACAACAACAAAGATGGTTTTCTCAGCACAAGGGAGCTAACAAAAGCTTTTGGTTTTCTAGGCTCAATTCTTCCATTCTACAAAGCTCACTATGGTTTGGCTTATGCAGATGCTGATAATGATGGTCTTATTAGTGAGGCTGAGCTTGACAAACTCGTTGATTATGCTAGTAAAGTCATGAAGAAGAAATGA

Coding sequence (CDS)

ATGTCTGTCTCCCAACTTCAAGCATGGGCTGAGGATGAAGCAAATGAATTGATCAAGAAGTATGATAAAAATGGTGATGGTGTTCTCACTAAAGATGAGCTACAATCCTTCCTCCACGATGTTCGAAGTTCAAATCAACTCAACAATCAAGGGTTCATATCTAAAATCCCAGAAATGAATGATGGTATTATGCACAAGCTTAGTTCAAACACTCATGTACAACCAAAAAAGCGATTACAAGACAAAGCTCAAAAATTCTCAGCTAAAGTTCTTGATAGAAAGGTATCTAATATGTCACTTAGTCAAGAGCAGATGAAGGAGATTTTCAAACATCATGACAACAACAAAGATGGTTTTCTCAGCACAAGGGAGCTAACAAAAGCTTTTGGTTTTCTAGGCTCAATTCTTCCATTCTACAAAGCTCACTATGGTTTGGCTTATGCAGATGCTGATAATGATGGTCTTATTAGTGAGGCTGAGCTTGACAAACTCGTTGATTATGCTAGTAAAGTCATGAAGAAGAAATGA

Protein sequence

MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMNDGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFLSTRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK
Homology
BLAST of Spg025189 vs. NCBI nr
Match: XP_023002691.1 (probable calcium-binding protein CML11 [Cucurbita maxima])

HSP 1 Score: 244.2 bits (622), Expect = 8.4e-61
Identity = 122/175 (69.71%), Postives = 147/175 (84.00%), Query Frame = 0

Query: 1   MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMN 60
           MSVS LQ WAE+EANEL+KKYDKNGDG+LTK+ELQ FL DVR S QLN QGF+SKIP+ N
Sbjct: 1   MSVSILQTWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSKIPKTN 60

Query: 61  DGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFL 120
           D   HKLSSNTH  PK ++QDK  K S KVL  KVSN+SLS+EQ+K+IFK++D++KDGFL
Sbjct: 61  DKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDGFL 120

Query: 121 STRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK 176
           + RE+TKAF FLGSI PFYKA Y + +ADA+NDGLISE EL+KL+DYA K++KKK
Sbjct: 121 NIREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISETELEKLIDYACKIIKKK 175

BLAST of Spg025189 vs. NCBI nr
Match: XP_038886759.1 (probable calcium-binding protein CML11 [Benincasa hispida])

HSP 1 Score: 244.2 bits (622), Expect = 8.4e-61
Identity = 122/177 (68.93%), Postives = 147/177 (83.05%), Query Frame = 0

Query: 1   MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFI--SKIPE 60
           MSVSQLQAWAE+EANELIKKYD+NGDGVL K+ELQSFLHD  SS QLNN   +    IP+
Sbjct: 1   MSVSQLQAWAENEANELIKKYDRNGDGVLAKEELQSFLHDTCSSTQLNNTRCVLSRMIPK 60

Query: 61  MNDGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDG 120
            N+ ++HKLSSNTH+  KK+L+DK Q  S K LDRK SN+  S+EQ+KEIFK+HD+NKDG
Sbjct: 61  TNNNLVHKLSSNTHMNQKKQLRDKIQNSSTKALDRKQSNIPPSREQIKEIFKYHDSNKDG 120

Query: 121 FLSTRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK 176
           FL+ RELTK FG LGS++PFYKAHY +AYADAD DGLISE ELD+L+DY +K++KKK
Sbjct: 121 FLNIRELTKTFGSLGSVIPFYKAHYAMAYADADKDGLISEVELDELIDYTNKIIKKK 177

BLAST of Spg025189 vs. NCBI nr
Match: XP_022951742.1 (probable calcium-binding protein CML11 [Cucurbita moschata])

HSP 1 Score: 240.4 bits (612), Expect = 1.2e-59
Identity = 122/175 (69.71%), Postives = 146/175 (83.43%), Query Frame = 0

Query: 1   MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMN 60
           MSVS LQAWAE++ANELIKKYDKNGDGVLTK+ELQ FL DVR S QLNNQGF+SKIP  N
Sbjct: 1   MSVSILQAWAENKANELIKKYDKNGDGVLTKEELQVFLRDVRGSTQLNNQGFLSKIPRTN 60

Query: 61  DGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFL 120
           D   HKL+SN    PK ++Q KA K S KVL  KVSN+ LS+EQ+K+IFK++D++KDGFL
Sbjct: 61  DKGAHKLNSNPRTNPKIQVQSKATKSSTKVLSEKVSNVPLSREQIKKIFKYYDSDKDGFL 120

Query: 121 STRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK 176
           + RE+TKAF FLGSI PFYKA Y + +ADA+NDGLISEAEL+KL+DYA K++KKK
Sbjct: 121 NIREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISEAELEKLIDYAYKIIKKK 175

BLAST of Spg025189 vs. NCBI nr
Match: XP_023538396.1 (probable calcium-binding protein CML11 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 240.0 bits (611), Expect = 1.6e-59
Identity = 121/175 (69.14%), Postives = 146/175 (83.43%), Query Frame = 0

Query: 1   MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMN 60
           MSVS LQAWAE+EANEL+KKYD+NGDGVLTK+ELQ FL DVR S QLNNQGF+SKIP+ N
Sbjct: 1   MSVSILQAWAENEANELMKKYDRNGDGVLTKEELQFFLRDVRGSTQLNNQGFLSKIPKTN 60

Query: 61  DGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFL 120
           D   HKLSSN H  PK ++Q KA   S KV   KVSN+ LS+EQ+K+IFK++D++KDGFL
Sbjct: 61  DKGAHKLSSNAHTNPKIQVQGKATNSSTKVFGEKVSNVPLSREQIKKIFKYYDSDKDGFL 120

Query: 121 STRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK 176
           + RE+TKAF FLGSI PFYKA Y + +ADA+NDGLISEAEL+KLVDYA K+++KK
Sbjct: 121 NRREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISEAELEKLVDYAGKIIQKK 175

BLAST of Spg025189 vs. NCBI nr
Match: XP_022951798.1 (probable calcium-binding protein CML11 [Cucurbita moschata])

HSP 1 Score: 238.0 bits (606), Expect = 6.0e-59
Identity = 120/175 (68.57%), Postives = 147/175 (84.00%), Query Frame = 0

Query: 1   MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMN 60
           MSVS LQAWAE+EANEL+KKYD+NGDGVLTK+ELQ FL DVR S QLN+Q F+SKIP+ N
Sbjct: 1   MSVSILQAWAENEANELMKKYDRNGDGVLTKEELQVFLRDVRGSTQLNHQRFLSKIPKTN 60

Query: 61  DGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFL 120
           D   H+LSSN H  PK ++Q  A K S KVL  KVSN+ LS+EQ+K+IFK++D++KDGFL
Sbjct: 61  DKGAHQLSSNAHTNPKIQVQGTATKSSTKVLSEKVSNVPLSREQIKKIFKYYDSDKDGFL 120

Query: 121 STRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK 176
           +TRE+TKAF FLGSI PFYKA Y + +ADA+NDGLISEAEL+KL+DYA K++KKK
Sbjct: 121 NTREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISEAELEKLIDYACKIIKKK 175

BLAST of Spg025189 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 8.0e-06
Identity = 44/168 (26.19%), Postives = 71/168 (42.26%), Query Frame = 0

Query: 5   QLQAWAEDEANELIKKYDKNGDGVLTKDELQSFL------------HDVRSSNQLNNQGF 64
           QL+     +  EL +++D NGDG LT+ EL + L            H + +    N  G 
Sbjct: 17  QLRGSQLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGS 76

Query: 65  ISKIPEMNDGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHH 124
           + +  E+   I   L++ TH+                          + Q Q+ E+F+  
Sbjct: 77  V-EFDELAAAIAPVLTTQTHL--------------------------VDQAQLLEVFRAF 136

Query: 125 DNNKDGFLSTRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAE 161
           D + +GF+S  EL ++   LG  L F +    +  AD D DG+IS  E
Sbjct: 137 DRDGNGFISAAELARSMARLGQPLTFEELTRMMRDADTDGDGVISFKE 157

BLAST of Spg025189 vs. ExPASy Swiss-Prot
Match: Q9AWK2 (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 GN=CML11 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 6.8e-05
Identity = 38/157 (24.20%), Postives = 68/157 (43.31%), Query Frame = 0

Query: 13  EANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMNDGIMHKLSSNTH 72
           E  E+ + +D+NGDG LT+ EL S L  +      +    + +  + N   + + S    
Sbjct: 64  ELREIFRSFDRNGDGSLTQLELGSLLRSLGLKPSTDELDSLIQRADTNSNGLIEFSEFVA 123

Query: 73  VQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFLSTRELTKAFGFL 132
           +   + L D+A                 S++Q++ +F   D + +GF++  EL  +   L
Sbjct: 124 LVAPELLYDRA---------------PYSEDQIRRLFNIFDRDGNGFITAAELAHSMAKL 183

Query: 133 GSILPFYKAHYGLAYADADNDGLISEAELDKLVDYAS 170
           G  L   +    +  AD D DG IS  E  + +  A+
Sbjct: 184 GHALTVKELTGMIKEADTDGDGRISFQEFSRAITAAA 205

BLAST of Spg025189 vs. ExPASy Swiss-Prot
Match: Q40642 (Calmodulin-like protein 1 OS=Oryza sativa subsp. indica OX=39946 GN=CML1 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.4e-04
Identity = 41/152 (26.97%), Postives = 70/152 (46.05%), Query Frame = 0

Query: 13  EANELIKKYDKNGDGVLTKDELQSFLHDV-RSSNQLNNQGFISKIPEMNDGIMHKLSSNT 72
           E  E    +DK+GDG +T  EL + +  + ++  +   Q  IS++            SN 
Sbjct: 12  EFREAFSLFDKDGDGSITTKELGTVMRSLGQNPTEAELQDMISEV---------DADSNG 71

Query: 73  HVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFLSTRELTKAFGF 132
           +++ K+ L   A+K   K           S+E++KE F+  D +++GF+S  EL      
Sbjct: 72  NIEFKEFLGLMARKLRDKD----------SEEELKEAFRVFDKDQNGFISAAELRHVMAN 131

Query: 133 LGSILPFYKAHYGLAYADADNDGLISEAELDK 164
           +G  L   +    ++ AD D DG I+  E  K
Sbjct: 132 IGERLTDEEVGEMISEADVDGDGQINYEEFVK 144

BLAST of Spg025189 vs. ExPASy Swiss-Prot
Match: Q8S1Y9 (Calmodulin-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CML1 PE=2 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 3.4e-04
Identity = 41/152 (26.97%), Postives = 70/152 (46.05%), Query Frame = 0

Query: 13  EANELIKKYDKNGDGVLTKDELQSFLHDV-RSSNQLNNQGFISKIPEMNDGIMHKLSSNT 72
           E  E    +DK+GDG +T  EL + +  + ++  +   Q  IS++            SN 
Sbjct: 12  EFREAFSLFDKDGDGSITTKELGTVMRSLGQNPTEAELQDMISEV---------DTDSNG 71

Query: 73  HVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFLSTRELTKAFGF 132
           +++ K+ L   A+K   K           S+E++KE F+  D +++GF+S  EL      
Sbjct: 72  NIEFKEFLGLMARKLRDKD----------SEEELKEAFRVFDKDQNGFISATELRHVMAN 131

Query: 133 LGSILPFYKAHYGLAYADADNDGLISEAELDK 164
           +G  L   +    ++ AD D DG I+  E  K
Sbjct: 132 IGERLTDEEVGEMISEADVDGDGQINYEEFVK 144

BLAST of Spg025189 vs. ExPASy TrEMBL
Match: A0A6J1KM01 (probable calcium-binding protein CML11 OS=Cucurbita maxima OX=3661 GN=LOC111496473 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 4.0e-61
Identity = 122/175 (69.71%), Postives = 147/175 (84.00%), Query Frame = 0

Query: 1   MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMN 60
           MSVS LQ WAE+EANEL+KKYDKNGDG+LTK+ELQ FL DVR S QLN QGF+SKIP+ N
Sbjct: 1   MSVSILQTWAENEANELMKKYDKNGDGILTKEELQVFLRDVRGSTQLNYQGFLSKIPKTN 60

Query: 61  DGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFL 120
           D   HKLSSNTH  PK ++QDK  K S KVL  KVSN+SLS+EQ+K+IFK++D++KDGFL
Sbjct: 61  DKGAHKLSSNTHTNPKIQVQDKVTKSSTKVLSEKVSNVSLSREQIKKIFKYYDSDKDGFL 120

Query: 121 STRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK 176
           + RE+TKAF FLGSI PFYKA Y + +ADA+NDGLISE EL+KL+DYA K++KKK
Sbjct: 121 NIREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISETELEKLIDYACKIIKKK 175

BLAST of Spg025189 vs. ExPASy TrEMBL
Match: A0A6J1GII5 (probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC111454483 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 5.8e-60
Identity = 122/175 (69.71%), Postives = 146/175 (83.43%), Query Frame = 0

Query: 1   MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMN 60
           MSVS LQAWAE++ANELIKKYDKNGDGVLTK+ELQ FL DVR S QLNNQGF+SKIP  N
Sbjct: 1   MSVSILQAWAENKANELIKKYDKNGDGVLTKEELQVFLRDVRGSTQLNNQGFLSKIPRTN 60

Query: 61  DGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFL 120
           D   HKL+SN    PK ++Q KA K S KVL  KVSN+ LS+EQ+K+IFK++D++KDGFL
Sbjct: 61  DKGAHKLNSNPRTNPKIQVQSKATKSSTKVLSEKVSNVPLSREQIKKIFKYYDSDKDGFL 120

Query: 121 STRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK 176
           + RE+TKAF FLGSI PFYKA Y + +ADA+NDGLISEAEL+KL+DYA K++KKK
Sbjct: 121 NIREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISEAELEKLIDYAYKIIKKK 175

BLAST of Spg025189 vs. ExPASy TrEMBL
Match: A0A6J1GIQ8 (probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC111454532 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.9e-59
Identity = 120/175 (68.57%), Postives = 147/175 (84.00%), Query Frame = 0

Query: 1   MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMN 60
           MSVS LQAWAE+EANEL+KKYD+NGDGVLTK+ELQ FL DVR S QLN+Q F+SKIP+ N
Sbjct: 1   MSVSILQAWAENEANELMKKYDRNGDGVLTKEELQVFLRDVRGSTQLNHQRFLSKIPKTN 60

Query: 61  DGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFL 120
           D   H+LSSN H  PK ++Q  A K S KVL  KVSN+ LS+EQ+K+IFK++D++KDGFL
Sbjct: 61  DKGAHQLSSNAHTNPKIQVQGTATKSSTKVLSEKVSNVPLSREQIKKIFKYYDSDKDGFL 120

Query: 121 STRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK 176
           +TRE+TKAF FLGSI PFYKA Y + +ADA+NDGLISEAEL+KL+DYA K++KKK
Sbjct: 121 NTREVTKAFSFLGSINPFYKAQYAMTFADANNDGLISEAELEKLIDYACKIIKKK 175

BLAST of Spg025189 vs. ExPASy TrEMBL
Match: A0A6J1KK82 (probable calcium-binding protein CML14 OS=Cucurbita maxima OX=3661 GN=LOC111496474 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.6e-57
Identity = 114/175 (65.14%), Postives = 144/175 (82.29%), Query Frame = 0

Query: 1   MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMN 60
           M VS LQ WAE+EANEL+KKYD+NGDGVLTK ELQ FL DVR S QLNNQGF+S+IP+ N
Sbjct: 1   MFVSILQVWAENEANELMKKYDRNGDGVLTKKELQIFLRDVRGSTQLNNQGFLSRIPKTN 60

Query: 61  DGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFL 120
           D + HKLSSN H  PK ++Q+KA K S KV    VS++ LS+EQ+K+IFK++D++KDGFL
Sbjct: 61  DNVAHKLSSNAHSNPKMQVQEKATKSSTKVFSENVSDVRLSREQIKKIFKYYDSDKDGFL 120

Query: 121 STRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK 176
           +  E+TKAF FLGSI PFYKA Y +++ADA+NDG+ISEAEL+KL+DYA K++ KK
Sbjct: 121 NITEVTKAFSFLGSINPFYKAQYAISFADANNDGVISEAELEKLIDYACKIIPKK 175

BLAST of Spg025189 vs. ExPASy TrEMBL
Match: A0A6J1KK48 (probable calcium-binding protein CML14 OS=Cucurbita maxima OX=3661 GN=LOC111496448 PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 3.5e-57
Identity = 116/175 (66.29%), Postives = 143/175 (81.71%), Query Frame = 0

Query: 1   MSVSQLQAWAEDEANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMN 60
           MSVS LQAWAE+EANEL+KKYD+NGDGVLTK+ELQ F  DVR S QLNNQGF+S IP+ N
Sbjct: 1   MSVSILQAWAENEANELMKKYDRNGDGVLTKEELQVFQRDVRGSTQLNNQGFLSNIPKTN 60

Query: 61  DGIMHKLSSNTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFL 120
           D   +KLSSN H  PK ++QDKA K S K    KVSN+ LS+EQ+K+IFK++D++KDGFL
Sbjct: 61  DKGANKLSSNAHTNPKIQVQDKATKSSTKAPSEKVSNVRLSREQIKKIFKYYDSDKDGFL 120

Query: 121 STRELTKAFGFLGSILPFYKAHYGLAYADADNDGLISEAELDKLVDYASKVMKKK 176
           + RE+TKAF FLGSI PFYKA Y + + DA+ND LI+EAEL+KL+DYA K++KKK
Sbjct: 121 NIREVTKAFSFLGSINPFYKAQYAMTFVDANNDSLINEAELEKLIDYACKIIKKK 175

BLAST of Spg025189 vs. TAIR 10
Match: AT2G41100.3 (Calcium-binding EF hand family protein )

HSP 1 Score: 51.6 bits (122), Expect = 7.4e-07
Identity = 43/165 (26.06%), Postives = 83/165 (50.30%), Query Frame = 0

Query: 13  EANELIKKYDKNGDGVLTKDELQSFLHDV-RSSNQLNNQGFISKIPEMNDGIMHKLSSNT 72
           E  E  + +DKNGDG +TK EL++ +  + ++  + + Q  ++++    DG +       
Sbjct: 66  EYRESFRLFDKNGDGSITKKELRTVMFSLGKNRTKADLQDMMNEVDLDGDGTI-DFPEFL 125

Query: 73  HVQPKKRLQDKAQKFSAK-VLDRKVSNMSLSQEQMKEIFKHHDNNKDGFLSTRELTKAFG 132
           ++  K +  D+A + + K ++D ++++  +   + +E F+  D N DG+++  EL     
Sbjct: 126 YLMAKNQGHDQAPRHTKKTMVDYQLTDDQIL--EFREAFRVFDKNGDGYITVNELRTTMR 185

Query: 133 FLGSILPFYKAHYGLAYADADNDGLISEAEL-----DKLVDYASK 171
            LG      +    +  ADAD DG IS +E       K++D  SK
Sbjct: 186 SLGETQTKAELQDMINEADADGDGTISFSEFVCVMTGKMIDTQSK 227

BLAST of Spg025189 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 44.3 bits (103), Expect = 1.2e-04
Identity = 42/153 (27.45%), Postives = 70/153 (45.75%), Query Frame = 0

Query: 13  EANELIKKYDKNGDGVLTKDELQSFLHDVRSSNQLNNQGFISKIPEMN-DGIMHKLSSNT 72
           E   + + +DKNGDG +TK+EL         ++ L N G    IP+ +   ++HK+ +N 
Sbjct: 65  ELKRVFQMFDKNGDGRITKEEL---------NDSLENLGIY--IPDKDLTQMIHKIDANG 124

Query: 73  HVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFLSTRELTKAFGF 132
                    D+ +   + ++D   ++    +E MK+ F   D + DGF++  EL      
Sbjct: 125 DGCVD---IDEFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMAS 184

Query: 133 LGSILPFYKAHYG----LAYADADNDGLISEAE 161
           LG  L   K   G    +   DAD DG ++  E
Sbjct: 185 LG--LKQGKTLDGCKKMIMQVDADGDGRVNYKE 201

BLAST of Spg025189 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 44.3 bits (103), Expect = 1.2e-04
Identity = 37/158 (23.42%), Postives = 68/158 (43.04%), Query Frame = 0

Query: 13  EANELIKKYDKNGDGVLTKDELQSFLHDVR---SSNQLNNQGFISKIPEMNDGIMHKLSS 72
           E + + + +DKNGDG + K+EL+ F   V      N++N    I+K+    DG M     
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINE--MIAKMDVNGDGAMD---- 64

Query: 73  NTHVQPKKRLQDKAQKFSAKVLDRKVSNMSLSQEQMKEIFKHHDNNKDGFLSTRELTKAF 132
                    + +    +   V +++       +E M+E F+  D N DGF++  EL    
Sbjct: 65  ---------IDEFGSLYQEMVEEKE------EEEDMREAFRVFDQNGDGFITDEELRSVL 124

Query: 133 GFLG--SILPFYKAHYGLAYADADNDGLISEAELDKLV 166
             +G             ++  D D DG+++  E  +++
Sbjct: 125 ASMGLKQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023002691.18.4e-6169.71probable calcium-binding protein CML11 [Cucurbita maxima][more]
XP_038886759.18.4e-6168.93probable calcium-binding protein CML11 [Benincasa hispida][more]
XP_022951742.11.2e-5969.71probable calcium-binding protein CML11 [Cucurbita moschata][more]
XP_023538396.11.6e-5969.14probable calcium-binding protein CML11 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022951798.16.0e-5968.57probable calcium-binding protein CML11 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q6L5F48.0e-0626.19Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9AWK26.8e-0524.20Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q406423.4e-0426.97Calmodulin-like protein 1 OS=Oryza sativa subsp. indica OX=39946 GN=CML1 PE=1 SV... [more]
Q8S1Y93.4e-0426.97Calmodulin-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CML1 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1KM014.0e-6169.71probable calcium-binding protein CML11 OS=Cucurbita maxima OX=3661 GN=LOC1114964... [more]
A0A6J1GII55.8e-6069.71probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1GIQ82.9e-5968.57probable calcium-binding protein CML11 OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1KK821.6e-5765.14probable calcium-binding protein CML14 OS=Cucurbita maxima OX=3661 GN=LOC1114964... [more]
A0A6J1KK483.5e-5766.29probable calcium-binding protein CML14 OS=Cucurbita maxima OX=3661 GN=LOC1114964... [more]
Match NameE-valueIdentityDescription
AT2G41100.37.4e-0726.06Calcium-binding EF hand family protein [more]
AT2G43290.11.2e-0427.45Calcium-binding EF-hand family protein [more]
AT4G12860.11.2e-0423.42EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 104..132
e-value: 1.2
score: 17.0
coord: 13..41
e-value: 8.2E-5
score: 32.0
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 13..39
e-value: 1.9E-6
score: 27.0
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 104..131
e-value: 9.6E-7
score: 28.1
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 148..165
e-value: 0.039
score: 13.4
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 100..135
score: 13.034948
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 9..44
score: 13.397589
NoneNo IPR availableGENE3D1.10.238.10coord: 45..171
e-value: 3.6E-11
score: 45.3
NoneNo IPR availableGENE3D1.10.238.10coord: 8..44
e-value: 4.4E-7
score: 31.4
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 85..170
NoneNo IPR availablePANTHERPTHR10891:SF911SUBFAMILY NOT NAMEDcoord: 85..170
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 113..125
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 22..34
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 149..161
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 6..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025189.1Spg025189.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding