Spg025074 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg025074
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionethylene-responsive transcription factor RAP2-3-like
Locationscaffold12: 9811054 .. 9812462 (+)
RNA-Seq ExpressionSpg025074
SyntenySpg025074
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGAAGAATCTGAGTGAAAGAAAAAGAGAGAAAGATGTGCGGTGGTGCCATCATCTCTGGTTTTATTGAAAAGAAGCGCAGTCGGAAGCTTAGTAATGGATCTCTCTGGTCCGACATCGACCCTTTTTCCGACCTCCTCGGCCTCGATTATTCCAATGGTATTTATCTCTTCATTTTACTTCAACTTTTTATTTTTTTTTTAAATCTTGGATAAATCATAAATCATTATTACCTGAGTATCGAATCATGGAGTAGAGTTAGACATTTTTTAGAAGAGGGGTAAAATTTTTAATTTTTTTTTAAATTCAAACAAACATGGAACATTGAGAATATATATATATCTTAATTAGTTGAACTATTTTGATGTTTTATCTACTTTATAGGATGAAGATCAAGTTGTTCATATTTTATTCCAATGGTTTGAAAAAAAAAACGATTCCTTTTTTAAAATGGTTATTTTATAGAGAGAATAAAAAATTAGTGTACTGGATTTAAAAAAAAAAAAAAATCCAGAAGGGAATGGAAATTTCGATCGTGATCGGTATTGAAGTGTGATTGGTTACTGGTTTGTAGTGAAAAGTGAGAAACCTCAGAAAAGAGAACCAGCTACGGCGGAGACGCCGCCGGAGAAGGCTCGAAAAACTCGGAAAAATATGTACAGAGGAATCCGGCAGCGGCCGTGGGGAAAATGGGCGGCGGAGATTCGGGACCCACATAAAGGCGTCCGAGTTTGGCTCGGCACTTTCAACACCGCCGAGGAAGCCGCCAGAGCCTACGATGAGGCGGCCAAAAGGATCCGAGGAGAAAAGGCAAAACTGAACTTCACTCCTCCGCCGCCGTCGCCGCCGTTACTGGAGGCTCAGCCTCAAATCCCGGCTAAAAAACGGTGCGTCCTTCCGGAACAGACTGGGGATTGTTTCAGTTCCACCGGCCCGCTGCCGCCTGGCACACGTGCGAATAACTCGTGTGAGAACGACGGGTTCTACTACGGCGAGGAATGGGCGAGTTTGAGATCCTTCCTGGGTCTGGATTGTGAAGACCAACAACCGAGTCAACTCGGTGGAAGTGACGAGTTTGACCGAGTTGCTGCAGACCTGTGGATGCTGGACGACATGTCGTTGAATGAGCAGAAATTCAGTCAAATGGTGTAAAACTCCGATTGACTCTTTTGTCCGACATGTCGTTTTACTGCTATGTACGTTTTGAATATTTTATGTGTTTTATTTGAAGTGTGTAGCCTGTAGATATATGGATAGATTGAATAAAAGCTAGCGATATGAACAATCCTTCGTTGTGTTTTTGTTTTCCTTGGAACGACATGTCGGTTCGTGTATCAGCTATCAGATCAGACTGCTTCTCTTTGTTTTTAATATTCACCGCTTTGAATGGATGGTCTTTGTGTTTGAC

mRNA sequence

ATGTGCGGTGGTGCCATCATCTCTGGTTTTATTGAAAAGAAGCGCAGTCGGAAGCTTAGTAATGGATCTCTCTGGTCCGACATCGACCCTTTTTCCGACCTCCTCGGCCTCGATTATTCCAATGTGAAAAGTGAGAAACCTCAGAAAAGAGAACCAGCTACGGCGGAGACGCCGCCGGAGAAGGCTCGAAAAACTCGGAAAAATATGTACAGAGGAATCCGGCAGCGGCCGTGGGGAAAATGGGCGGCGGAGATTCGGGACCCACATAAAGGCGTCCGAGTTTGGCTCGGCACTTTCAACACCGCCGAGGAAGCCGCCAGAGCCTACGATGAGGCGGCCAAAAGGATCCGAGGAGAAAAGGCAAAACTGAACTTCACTCCTCCGCCGCCGTCGCCGCCGTTACTGGAGGCTCAGCCTCAAATCCCGGCTAAAAAACGGTGCGTCCTTCCGGAACAGACTGGGGATTGTTTCAGTTCCACCGGCCCGCTGCCGCCTGGCACACGTGCGAATAACTCGTGTGAGAACGACGGGTTCTACTACGGCGAGGAATGGGCGAGTTTGAGATCCTTCCTGGGTCTGGATTGTGAAGACCAACAACCGAGTCAACTCGGTGGAAGTGACGAGTTTGACCGAGTTGCTGCAGACCTGTGGATGCTGGACGACATGTCGTTGAATGAGCAGAAATTCAGTCAAATGGTGTAA

Coding sequence (CDS)

ATGTGCGGTGGTGCCATCATCTCTGGTTTTATTGAAAAGAAGCGCAGTCGGAAGCTTAGTAATGGATCTCTCTGGTCCGACATCGACCCTTTTTCCGACCTCCTCGGCCTCGATTATTCCAATGTGAAAAGTGAGAAACCTCAGAAAAGAGAACCAGCTACGGCGGAGACGCCGCCGGAGAAGGCTCGAAAAACTCGGAAAAATATGTACAGAGGAATCCGGCAGCGGCCGTGGGGAAAATGGGCGGCGGAGATTCGGGACCCACATAAAGGCGTCCGAGTTTGGCTCGGCACTTTCAACACCGCCGAGGAAGCCGCCAGAGCCTACGATGAGGCGGCCAAAAGGATCCGAGGAGAAAAGGCAAAACTGAACTTCACTCCTCCGCCGCCGTCGCCGCCGTTACTGGAGGCTCAGCCTCAAATCCCGGCTAAAAAACGGTGCGTCCTTCCGGAACAGACTGGGGATTGTTTCAGTTCCACCGGCCCGCTGCCGCCTGGCACACGTGCGAATAACTCGTGTGAGAACGACGGGTTCTACTACGGCGAGGAATGGGCGAGTTTGAGATCCTTCCTGGGTCTGGATTGTGAAGACCAACAACCGAGTCAACTCGGTGGAAGTGACGAGTTTGACCGAGTTGCTGCAGACCTGTGGATGCTGGACGACATGTCGTTGAATGAGCAGAAATTCAGTCAAATGGTGTAA

Protein sequence

MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATAETPPEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCENDGFYYGEEWASLRSFLGLDCEDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQKFSQMV
Homology
BLAST of Spg025074 vs. NCBI nr
Match: XP_008458405.1 (PREDICTED: ethylene-responsive transcription factor RAP2-3-like [Cucumis melo])

HSP 1 Score: 336.7 bits (862), Expect = 1.6e-88
Identity = 173/237 (73.00%), Postives = 197/237 (83.12%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATA----E 60
           MCGGAIIS FIEK+R+ KL+N SLWS IDPFSDLLGL+YS+VK+EKP+KR+ +TA     
Sbjct: 20  MCGGAIISDFIEKRRTCKLNNESLWSHIDPFSDLLGLNYSSVKTEKPKKRDRSTAAEAVA 79

Query: 61  TPPEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRI 120
              EK RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAKRI
Sbjct: 80  VAVEKTRKVRKNLYRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAARAYDEAAKRI 139

Query: 121 RGEKAKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCEND 180
           RG KAKLNF P PPSP    AQPQ P KK+CVLPE T   F++T   PP TRA++SC+++
Sbjct: 140 RGVKAKLNFAPAPPSP---TAQPQTPVKKQCVLPELTPGGFNTTDS-PPCTRADSSCDSE 199

Query: 181 GFYYGEEWASLRSFLGLDCEDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQKFSQMV 234
             YYGEE ASLRSFLGLD E+QQPSQLGG+ EF+RV  DLWM+DDMSLNE+KFSQ+V
Sbjct: 200 ELYYGEELASLRSFLGLDGEEQQPSQLGGNGEFERVDLDLWMMDDMSLNEEKFSQLV 252

BLAST of Spg025074 vs. NCBI nr
Match: KAG6593985.1 (Ethylene-responsive transcription factor RAP2-3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026326.1 Ethylene-responsive transcription factor RAP2-3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 331.6 bits (849), Expect = 5.3e-87
Identity = 174/234 (74.36%), Postives = 187/234 (79.91%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATAETPPE 60
           MCGGA+ISG IEKKR RKLSNGSLW D D FSDLLGLDY  +K+E  QK   ATA   PE
Sbjct: 1   MCGGALISGIIEKKRGRKLSNGSLWPDTDHFSDLLGLDYCALKTETSQKECRATAVPEPE 60

Query: 61  KARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEK 120
           K RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAKRIRGEK
Sbjct: 61  KTRKARKNIYRGIRQRPWGKWAAEIRDPQKGVRVWLGTFNTAEEAARAYDEAAKRIRGEK 120

Query: 121 AKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCENDGFYY 180
           AKLNF P P   PL  +QP  PAKKRCV  E T DCFSST P P  TR NNSCE DG YY
Sbjct: 121 AKLNFAPRP--QPLPVSQP--PAKKRCVAHELTSDCFSSTSPAPASTRVNNSCEIDGLYY 180

Query: 181 GEEWASLRSFLGLDC-EDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQKFSQMV 234
           GEE ASLRSFL LD  E+QQPSQ+GGSDEF + A +LWM+DD SLNE+KF ++V
Sbjct: 181 GEELASLRSFLDLDFEEEQQPSQVGGSDEFGQ-AVELWMMDDPSLNEEKFGEVV 229

BLAST of Spg025074 vs. NCBI nr
Match: XP_023000151.1 (ethylene-responsive transcription factor RAP2-3 [Cucurbita maxima])

HSP 1 Score: 331.6 bits (849), Expect = 5.3e-87
Identity = 177/238 (74.37%), Postives = 190/238 (79.83%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATAET--- 60
           MCGGA+ISG IEKKR RKLSNGSLW D D FSDLLGLDY  +K+E PQK+  ATAET   
Sbjct: 1   MCGGALISGIIEKKRGRKLSNGSLWPDTDHFSDLLGLDYCALKTETPQKKCRATAETAAV 60

Query: 61  -PPEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRI 120
             PEK RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAKRI
Sbjct: 61  PEPEKTRKARKNIYRGIRQRPWGKWAAEIRDPQKGVRVWLGTFNTAEEAARAYDEAAKRI 120

Query: 121 RGEKAKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCEND 180
           RGEKAKLNF P P   PL  +QP  PAKKRCV  E T DCFSST   P  TR NNSCE D
Sbjct: 121 RGEKAKLNFAPRP--QPLPVSQP--PAKKRCVAHELTPDCFSSTSSAPASTRVNNSCEID 180

Query: 181 GFYYGEEWASLRSFLGLDC-EDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQKFSQMV 234
           G YYGEE ASLRSFL LD  E+QQPSQ+GGSDEF + A +LWM+DD SLNE+KFS +V
Sbjct: 181 GLYYGEELASLRSFLDLDFEEEQQPSQVGGSDEFGQ-AVELWMMDDPSLNEEKFSSVV 233

BLAST of Spg025074 vs. NCBI nr
Match: KGN47418.1 (hypothetical protein Csa_023005 [Cucumis sativus])

HSP 1 Score: 328.6 bits (841), Expect = 4.5e-86
Identity = 170/235 (72.34%), Postives = 193/235 (82.13%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATA--ETP 60
           MCGGAIIS FIEKKR+ KL+N SLWS IDPFSDLLGL+YS+VK+E P+KR  +TA     
Sbjct: 1   MCGGAIISDFIEKKRTCKLNNESLWSHIDPFSDLLGLNYSSVKTETPKKRNRSTAAEAVA 60

Query: 61  PEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRG 120
            EK RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAKRIRG
Sbjct: 61  VEKTRKARKNIYRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAARAYDEAAKRIRG 120

Query: 121 EKAKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCENDGF 180
           EKAKLNF P PPS   L AQ Q P K +CV+PE T    ++TG  PP TRA++SC+++  
Sbjct: 121 EKAKLNFAPAPPS---LAAQRQTPVKMQCVVPEFTPSGLNTTGS-PPCTRADSSCDSEEL 180

Query: 181 YYGEEWASLRSFLGLDCEDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQKFSQMV 234
           YYGEE ASLRSFLGLD E+QQ S+LGG+ EF+RV  D+WM+DDMSLNEQKFSQMV
Sbjct: 181 YYGEELASLRSFLGLDSEEQQASELGGNGEFERVDLDVWMMDDMSLNEQKFSQMV 231

BLAST of Spg025074 vs. NCBI nr
Match: XP_004153075.3 (ethylene-responsive transcription factor RAP2-3 [Cucumis sativus])

HSP 1 Score: 328.6 bits (841), Expect = 4.5e-86
Identity = 170/235 (72.34%), Postives = 193/235 (82.13%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATA--ETP 60
           MCGGAIIS FIEKKR+ KL+N SLWS IDPFSDLLGL+YS+VK+E P+KR  +TA     
Sbjct: 20  MCGGAIISDFIEKKRTCKLNNESLWSHIDPFSDLLGLNYSSVKTETPKKRNRSTAAEAVA 79

Query: 61  PEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRG 120
            EK RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAKRIRG
Sbjct: 80  VEKTRKARKNIYRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAARAYDEAAKRIRG 139

Query: 121 EKAKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCENDGF 180
           EKAKLNF P PPS   L AQ Q P K +CV+PE T    ++TG  PP TRA++SC+++  
Sbjct: 140 EKAKLNFAPAPPS---LAAQRQTPVKMQCVVPEFTPSGLNTTGS-PPCTRADSSCDSEEL 199

Query: 181 YYGEEWASLRSFLGLDCEDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQKFSQMV 234
           YYGEE ASLRSFLGLD E+QQ S+LGG+ EF+RV  D+WM+DDMSLNEQKFSQMV
Sbjct: 200 YYGEELASLRSFLGLDSEEQQASELGGNGEFERVDLDVWMMDDMSLNEQKFSQMV 250

BLAST of Spg025074 vs. ExPASy Swiss-Prot
Match: P42736 (Ethylene-responsive transcription factor RAP2-3 OS=Arabidopsis thaliana OX=3702 GN=RAP2-3 PE=1 SV=2)

HSP 1 Score: 169.1 bits (427), Expect = 6.0e-41
Identity = 118/256 (46.09%), Postives = 143/256 (55.86%), Query Frame = 0

Query: 1   MCGGAIISGF---IEKKRSRKLSNGSLWSDID-----------PFSDLLGLDYSNVKSEK 60
           MCGGAIIS +   + K + RKL+   LWS++D             S L   +  NVK E 
Sbjct: 1   MCGGAIISDYAPLVTKAKGRKLTAEELWSELDASAADDFWGFYSTSKLHPTNQVNVKEEA 60

Query: 61  PQKREPATAETPPEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAA 120
            +K +     T P K RK RKN+YRGIR+RPWGKWAAEIRDP KGVRVWLGTFNTAEEAA
Sbjct: 61  VKKEQ----ATEPGKRRK-RKNVYRGIRKRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAA 120

Query: 121 RAYDEAAKRIRGEKAKLNF-----------TPPPPSPPLLEAQPQIPAKKRCVLPEQTGD 180
            AYD AAK+IRG+KAKLNF           TPPP SP    +  Q PAKK CV+ +   +
Sbjct: 121 MAYDVAAKQIRGDKAKLNFPDLHHPPPPNYTPPPSSP---RSTDQPPAKKVCVVSQSESE 180

Query: 181 CFSSTGPLPPGTRANNSCENDGFYYG--------EEWASLRSFLGLDCED-QQPSQLGGS 223
               + P+       N  E     YG        ++ +SL SFL LD    +QPSQL  S
Sbjct: 181 LSQPSFPV-ECIGFGNGDEFQNLSYGFEPDYDLKQQISSLESFLELDGNTAEQPSQLDES 240

BLAST of Spg025074 vs. ExPASy Swiss-Prot
Match: O22259 (Ethylene-responsive transcription factor ERF071 OS=Arabidopsis thaliana OX=3702 GN=ERF071 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.3e-27
Identity = 72/125 (57.60%), Postives = 82/125 (65.60%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATAETPPE 60
           MCGGAIIS FI             WS  +     LG    +V S K  KR+P +  +   
Sbjct: 1   MCGGAIISDFI-------------WSKSESEPSQLG----SVSSRK--KRKPVSV-SEER 60

Query: 61  KARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEK 120
             ++ RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTF TA+EAARAYD AA +IRG K
Sbjct: 61  DGKRERKNLYRGIRQRPWGKWAAEIRDPSKGVRVWLGTFKTADEAARAYDVAAIKIRGRK 105

Query: 121 AKLNF 126
           AKLNF
Sbjct: 121 AKLNF 105

BLAST of Spg025074 vs. ExPASy Swiss-Prot
Match: Q9LUM4 (Ethylene-responsive transcription factor RAP2-2 OS=Arabidopsis thaliana OX=3702 GN=RAP2-2 PE=1 SV=2)

HSP 1 Score: 122.1 bits (305), Expect = 8.4e-27
Identity = 83/202 (41.09%), Postives = 103/202 (50.99%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDI-----------DPFSDLLGLD----------- 60
           MCGGAIIS FI   RS +++N  +W D+           +  SD   LD           
Sbjct: 1   MCGGAIISDFIPPPRSLRVTNEFIWPDLKNKVKASKKRSNKRSDFFDLDDDFEADFQGFK 60

Query: 61  -------------YSNVK------SEKP-----------------QKREPATAETPPEKA 120
                        + NVK      + KP                  K+   +AE   + +
Sbjct: 61  DDSAFDCEDDDDVFVNVKPFVFTATTKPVASAFVSTGIYLVGSAYAKKTVESAEQAEKSS 120

Query: 121 RKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAK 145
           ++ RKN YRGIRQRPWGKWAAEIRDP KG R WLGTF+TAEEAARAYD AA+RIRG KAK
Sbjct: 121 KRKRKNQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAYDAAARRIRGTKAK 180

BLAST of Spg025074 vs. ExPASy Swiss-Prot
Match: Q8H0T5 (Ethylene-responsive transcription factor ERF073 OS=Arabidopsis thaliana OX=3702 GN=ERF073 PE=1 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 1.4e-26
Identity = 67/132 (50.76%), Postives = 84/132 (63.64%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFS----DLLGLDYSNVKSEKP---QKREPA 60
           MCGGA+IS +I  ++  + S  S W     F     D  G ++  ++S++P         
Sbjct: 1   MCGGAVISDYIAPEKIARSSGKSSWRSNGVFDCSIYDFDG-NFDELESDEPFVFSSTHKH 60

Query: 61  TAETPPEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAA 120
            A       +K + + Y+GIR+RPWG+WAAEIRDP KGVRVWLGTFNTAEEAARAYD  A
Sbjct: 61  HASGSASDGKKKQSSRYKGIRRRPWGRWAAEIRDPIKGVRVWLGTFNTAEEAARAYDLEA 120

Query: 121 KRIRGEKAKLNF 126
           KRIRG KAKLNF
Sbjct: 121 KRIRGAKAKLNF 131

BLAST of Spg025074 vs. ExPASy Swiss-Prot
Match: Q9SSA8 (Ethylene-responsive transcription factor RAP2-12 OS=Arabidopsis thaliana OX=3702 GN=RAP2-12 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.9e-26
Identity = 75/180 (41.67%), Postives = 93/180 (51.67%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLW----------------------------SDIDPFS 60
           MCGGAIIS FI   RSR++++  +W                            +D   F 
Sbjct: 1   MCGGAIISDFIPPPRSRRVTSEFIWPDLKKNLKGSKKSSKNRSNFFDFDAEFEADFQGFK 60

Query: 61  DLLGLD----------YSNVK----SEKPQKREPATAE-------------TPPEKARKT 120
           D   +D          +++VK    +  P+    A AE                + A + 
Sbjct: 61  DDSSIDCDDDFDVGDVFADVKPFVFTSTPKPAVSAAAEGSVFGKKVTGLDGDAEKSANRK 120

Query: 121 RKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAKLNF 126
           RKN YRGIRQRPWGKWAAEIRDP +G R+WLGTF TAEEAARAYD AA+RIRG KAK+NF
Sbjct: 121 RKNQYRGIRQRPWGKWAAEIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSKAKVNF 180

BLAST of Spg025074 vs. ExPASy TrEMBL
Match: A0A1S3C7W9 (ethylene-responsive transcription factor RAP2-3-like OS=Cucumis melo OX=3656 GN=LOC103497827 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 8.0e-89
Identity = 173/237 (73.00%), Postives = 197/237 (83.12%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATA----E 60
           MCGGAIIS FIEK+R+ KL+N SLWS IDPFSDLLGL+YS+VK+EKP+KR+ +TA     
Sbjct: 20  MCGGAIISDFIEKRRTCKLNNESLWSHIDPFSDLLGLNYSSVKTEKPKKRDRSTAAEAVA 79

Query: 61  TPPEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRI 120
              EK RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAKRI
Sbjct: 80  VAVEKTRKVRKNLYRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAARAYDEAAKRI 139

Query: 121 RGEKAKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCEND 180
           RG KAKLNF P PPSP    AQPQ P KK+CVLPE T   F++T   PP TRA++SC+++
Sbjct: 140 RGVKAKLNFAPAPPSP---TAQPQTPVKKQCVLPELTPGGFNTTDS-PPCTRADSSCDSE 199

Query: 181 GFYYGEEWASLRSFLGLDCEDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQKFSQMV 234
             YYGEE ASLRSFLGLD E+QQPSQLGG+ EF+RV  DLWM+DDMSLNE+KFSQ+V
Sbjct: 200 ELYYGEELASLRSFLGLDGEEQQPSQLGGNGEFERVDLDLWMMDDMSLNEEKFSQLV 252

BLAST of Spg025074 vs. ExPASy TrEMBL
Match: A0A6J1KF31 (ethylene-responsive transcription factor RAP2-3 OS=Cucurbita maxima OX=3661 GN=LOC111494435 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 2.6e-87
Identity = 177/238 (74.37%), Postives = 190/238 (79.83%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATAET--- 60
           MCGGA+ISG IEKKR RKLSNGSLW D D FSDLLGLDY  +K+E PQK+  ATAET   
Sbjct: 1   MCGGALISGIIEKKRGRKLSNGSLWPDTDHFSDLLGLDYCALKTETPQKKCRATAETAAV 60

Query: 61  -PPEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRI 120
             PEK RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAKRI
Sbjct: 61  PEPEKTRKARKNIYRGIRQRPWGKWAAEIRDPQKGVRVWLGTFNTAEEAARAYDEAAKRI 120

Query: 121 RGEKAKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCEND 180
           RGEKAKLNF P P   PL  +QP  PAKKRCV  E T DCFSST   P  TR NNSCE D
Sbjct: 121 RGEKAKLNFAPRP--QPLPVSQP--PAKKRCVAHELTPDCFSSTSSAPASTRVNNSCEID 180

Query: 181 GFYYGEEWASLRSFLGLDC-EDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQKFSQMV 234
           G YYGEE ASLRSFL LD  E+QQPSQ+GGSDEF + A +LWM+DD SLNE+KFS +V
Sbjct: 181 GLYYGEELASLRSFLDLDFEEEQQPSQVGGSDEFGQ-AVELWMMDDPSLNEEKFSSVV 233

BLAST of Spg025074 vs. ExPASy TrEMBL
Match: A0A0A0KD18 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G318160 PE=4 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 2.2e-86
Identity = 170/235 (72.34%), Postives = 193/235 (82.13%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATA--ETP 60
           MCGGAIIS FIEKKR+ KL+N SLWS IDPFSDLLGL+YS+VK+E P+KR  +TA     
Sbjct: 1   MCGGAIISDFIEKKRTCKLNNESLWSHIDPFSDLLGLNYSSVKTETPKKRNRSTAAEAVA 60

Query: 61  PEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRG 120
            EK RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAKRIRG
Sbjct: 61  VEKTRKARKNIYRGIRQRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAARAYDEAAKRIRG 120

Query: 121 EKAKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCENDGF 180
           EKAKLNF P PPS   L AQ Q P K +CV+PE T    ++TG  PP TRA++SC+++  
Sbjct: 121 EKAKLNFAPAPPS---LAAQRQTPVKMQCVVPEFTPSGLNTTGS-PPCTRADSSCDSEEL 180

Query: 181 YYGEEWASLRSFLGLDCEDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQKFSQMV 234
           YYGEE ASLRSFLGLD E+QQ S+LGG+ EF+RV  D+WM+DDMSLNEQKFSQMV
Sbjct: 181 YYGEELASLRSFLGLDSEEQQASELGGNGEFERVDLDVWMMDDMSLNEQKFSQMV 231

BLAST of Spg025074 vs. ExPASy TrEMBL
Match: A0A6J1ER95 (ethylene-responsive transcription factor RAP2-3 OS=Cucurbita moschata OX=3662 GN=LOC111436844 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 4.8e-86
Identity = 171/234 (73.08%), Postives = 186/234 (79.49%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATAETPPE 60
           MCGG++ISG IEKKR RKL NGSLW D D FSDLLGLDY  +K+E  QK   ATA   PE
Sbjct: 1   MCGGSLISGIIEKKRGRKLRNGSLWPDTDHFSDLLGLDYCALKTETSQKECRATAVPEPE 60

Query: 61  KARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEK 120
           K RK RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTFNTAEEAARAYDEAAKRIRGEK
Sbjct: 61  KTRKARKNIYRGIRQRPWGKWAAEIRDPQKGVRVWLGTFNTAEEAARAYDEAAKRIRGEK 120

Query: 121 AKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCENDGFYY 180
           AKLNF P P   PL  +QP  PAKKRCV  E T DCFSST P P  TR NNSCE DG YY
Sbjct: 121 AKLNFAPRP--QPLPVSQP--PAKKRCVAHELTSDCFSSTSPAPASTRVNNSCEIDGLYY 180

Query: 181 GEEWASLRSFLGLDC-EDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQKFSQMV 234
           GEE ASLRSFL LD  E+Q+PSQ+GGSDEF + A +LWM+DD SLNE+KF ++V
Sbjct: 181 GEELASLRSFLDLDFEEEQEPSQVGGSDEFGQ-AVELWMMDDPSLNEEKFGEVV 229

BLAST of Spg025074 vs. ExPASy TrEMBL
Match: A0A6J1CA63 (ethylene-responsive transcription factor RAP2-3-like OS=Momordica charantia OX=3673 GN=LOC111009718 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 4.1e-85
Identity = 177/246 (71.95%), Postives = 191/246 (77.64%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKRE--PATAETP 60
           MCGGAIIS FIEKKRSRK SNG LWS+IDPFSDLLGLD+S+VKSEKPQKRE  PA A   
Sbjct: 1   MCGGAIISDFIEKKRSRKFSNGDLWSEIDPFSDLLGLDFSSVKSEKPQKRERSPAVAAEG 60

Query: 61  PE-KARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIR 120
            E K RKTRKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTF+TAEEAARAYDEAAKRIR
Sbjct: 61  IELKPRKTRKNLYRGIRQRPWGKWAAEIRDPQKGVRVWLGTFSTAEEAARAYDEAAKRIR 120

Query: 121 GEKAKLNFTPPPPSPPLLEAQPQIPAKKRCVLPEQTGDCFSSTGPLPPGTRANNSCENDG 180
           GEKAKLNF P         AQPQ PAKKRCVLPE T +  ++TGP P  +R +NS  NDG
Sbjct: 121 GEKAKLNFAP--------AAQPQAPAKKRCVLPELTREGLNTTGP-PTRSRTSNS-YNDG 180

Query: 181 FYYGEEWAS----------LRSFLGLDCEDQQPSQLGGSDEFDRVAADLWMLDDMSLNEQ 234
            YYG+E AS          L SFLGLD EDQQ SQLGGSDEFDRV  DLWM+DD SLN Q
Sbjct: 181 IYYGDELASGFELKEQISNLTSFLGLDGEDQQLSQLGGSDEFDRVVVDLWMMDDRSLNRQ 236

BLAST of Spg025074 vs. TAIR 10
Match: AT3G16770.1 (ethylene-responsive element binding protein )

HSP 1 Score: 169.1 bits (427), Expect = 4.2e-42
Identity = 118/256 (46.09%), Postives = 143/256 (55.86%), Query Frame = 0

Query: 1   MCGGAIISGF---IEKKRSRKLSNGSLWSDID-----------PFSDLLGLDYSNVKSEK 60
           MCGGAIIS +   + K + RKL+   LWS++D             S L   +  NVK E 
Sbjct: 1   MCGGAIISDYAPLVTKAKGRKLTAEELWSELDASAADDFWGFYSTSKLHPTNQVNVKEEA 60

Query: 61  PQKREPATAETPPEKARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAA 120
            +K +     T P K RK RKN+YRGIR+RPWGKWAAEIRDP KGVRVWLGTFNTAEEAA
Sbjct: 61  VKKEQ----ATEPGKRRK-RKNVYRGIRKRPWGKWAAEIRDPRKGVRVWLGTFNTAEEAA 120

Query: 121 RAYDEAAKRIRGEKAKLNF-----------TPPPPSPPLLEAQPQIPAKKRCVLPEQTGD 180
            AYD AAK+IRG+KAKLNF           TPPP SP    +  Q PAKK CV+ +   +
Sbjct: 121 MAYDVAAKQIRGDKAKLNFPDLHHPPPPNYTPPPSSP---RSTDQPPAKKVCVVSQSESE 180

Query: 181 CFSSTGPLPPGTRANNSCENDGFYYG--------EEWASLRSFLGLDCED-QQPSQLGGS 223
               + P+       N  E     YG        ++ +SL SFL LD    +QPSQL  S
Sbjct: 181 LSQPSFPV-ECIGFGNGDEFQNLSYGFEPDYDLKQQISSLESFLELDGNTAEQPSQLDES 240

BLAST of Spg025074 vs. TAIR 10
Match: AT2G47520.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 9.2e-29
Identity = 72/125 (57.60%), Postives = 82/125 (65.60%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDIDPFSDLLGLDYSNVKSEKPQKREPATAETPPE 60
           MCGGAIIS FI             WS  +     LG    +V S K  KR+P +  +   
Sbjct: 1   MCGGAIISDFI-------------WSKSESEPSQLG----SVSSRK--KRKPVSV-SEER 60

Query: 61  KARKTRKNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEK 120
             ++ RKN+YRGIRQRPWGKWAAEIRDP KGVRVWLGTF TA+EAARAYD AA +IRG K
Sbjct: 61  DGKRERKNLYRGIRQRPWGKWAAEIRDPSKGVRVWLGTFKTADEAARAYDVAAIKIRGRK 105

Query: 121 AKLNF 126
           AKLNF
Sbjct: 121 AKLNF 105

BLAST of Spg025074 vs. TAIR 10
Match: AT3G14230.3 (related to AP2 2 )

HSP 1 Score: 124.0 bits (310), Expect = 1.6e-28
Identity = 83/197 (42.13%), Postives = 103/197 (52.28%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDI-----------DPFSDLLGLD----------- 60
           MCGGAIIS FI   RS +++N  +W D+           +  SD   LD           
Sbjct: 1   MCGGAIISDFIPPPRSLRVTNEFIWPDLKNKVKASKKRSNKRSDFFDLDDDFEADFQGFK 60

Query: 61  -------------YSNVK------SEKP------------QKREPATAETPPEKARKTRK 120
                        + NVK      + KP             K+   +AE   + +++ RK
Sbjct: 61  DDSAFDCEDDDDVFVNVKPFVFTATTKPVASAFVSTGSAYAKKTVESAEQAEKSSKRKRK 120

Query: 121 NMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAKLNFTP 145
           N YRGIRQRPWGKWAAEIRDP KG R WLGTF+TAEEAARAYD AA+RIRG KAK+NF  
Sbjct: 121 NQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAYDAAARRIRGTKAKVNF-- 180

BLAST of Spg025074 vs. TAIR 10
Match: AT3G14230.2 (related to AP2 2 )

HSP 1 Score: 123.6 bits (309), Expect = 2.0e-28
Identity = 83/198 (41.92%), Postives = 103/198 (52.02%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLWSDI-----------DPFSDLLGLD----------- 60
           MCGGAIIS FI   RS +++N  +W D+           +  SD   LD           
Sbjct: 1   MCGGAIISDFIPPPRSLRVTNEFIWPDLKNKVKASKKRSNKRSDFFDLDDDFEADFQGFK 60

Query: 61  -------------YSNVK------SEKP-------------QKREPATAETPPEKARKTR 120
                        + NVK      + KP              K+   +AE   + +++ R
Sbjct: 61  DDSAFDCEDDDDVFVNVKPFVFTATTKPVASAFVSTVGSAYAKKTVESAEQAEKSSKRKR 120

Query: 121 KNMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAKLNFT 145
           KN YRGIRQRPWGKWAAEIRDP KG R WLGTF+TAEEAARAYD AA+RIRG KAK+NF 
Sbjct: 121 KNQYRGIRQRPWGKWAAEIRDPRKGSREWLGTFDTAEEAARAYDAAARRIRGTKAKVNF- 180

BLAST of Spg025074 vs. TAIR 10
Match: AT1G53910.3 (related to AP2 12 )

HSP 1 Score: 122.5 bits (306), Expect = 4.6e-28
Identity = 76/178 (42.70%), Postives = 94/178 (52.81%), Query Frame = 0

Query: 1   MCGGAIISGFIEKKRSRKLSNGSLW----------------------------SDIDPFS 60
           MCGGAIIS FI   RSR++++  +W                            +D   F 
Sbjct: 1   MCGGAIISDFIPPPRSRRVTSEFIWPDLKKNLKGSKKSSKNRSNFFDFDAEFEADFQGFK 60

Query: 61  DLLGLD----------YSNVK----SEKPQKREPATAETPPEK-----------ARKTRK 120
           D   +D          +++VK    +  P+    A AE   +K           A + RK
Sbjct: 61  DDSSIDCDDDFDVGDVFADVKPFVFTSTPKPAVSAAAEVFGKKVTGLDGDAEKSANRKRK 120

Query: 121 NMYRGIRQRPWGKWAAEIRDPHKGVRVWLGTFNTAEEAARAYDEAAKRIRGEKAKLNF 126
           N YRGIRQRPWGKWAAEIRDP +G R+WLGTF TAEEAARAYD AA+RIRG KAK+NF
Sbjct: 121 NQYRGIRQRPWGKWAAEIRDPREGARIWLGTFKTAEEAARAYDAAARRIRGSKAKVNF 178

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008458405.11.6e-8873.00PREDICTED: ethylene-responsive transcription factor RAP2-3-like [Cucumis melo][more]
KAG6593985.15.3e-8774.36Ethylene-responsive transcription factor RAP2-3, partial [Cucurbita argyrosperma... [more]
XP_023000151.15.3e-8774.37ethylene-responsive transcription factor RAP2-3 [Cucurbita maxima][more]
KGN47418.14.5e-8672.34hypothetical protein Csa_023005 [Cucumis sativus][more]
XP_004153075.34.5e-8672.34ethylene-responsive transcription factor RAP2-3 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
P427366.0e-4146.09Ethylene-responsive transcription factor RAP2-3 OS=Arabidopsis thaliana OX=3702 ... [more]
O222591.3e-2757.60Ethylene-responsive transcription factor ERF071 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LUM48.4e-2741.09Ethylene-responsive transcription factor RAP2-2 OS=Arabidopsis thaliana OX=3702 ... [more]
Q8H0T51.4e-2650.76Ethylene-responsive transcription factor ERF073 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9SSA81.9e-2641.67Ethylene-responsive transcription factor RAP2-12 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A1S3C7W98.0e-8973.00ethylene-responsive transcription factor RAP2-3-like OS=Cucumis melo OX=3656 GN=... [more]
A0A6J1KF312.6e-8774.37ethylene-responsive transcription factor RAP2-3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0KD182.2e-8672.34AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G318160 PE=... [more]
A0A6J1ER954.8e-8673.08ethylene-responsive transcription factor RAP2-3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1CA634.1e-8571.95ethylene-responsive transcription factor RAP2-3-like OS=Momordica charantia OX=3... [more]
Match NameE-valueIdentityDescription
AT3G16770.14.2e-4246.09ethylene-responsive element binding protein [more]
AT2G47520.19.2e-2957.60Integrase-type DNA-binding superfamily protein [more]
AT3G14230.31.6e-2842.13related to AP2 2 [more]
AT3G14230.22.0e-2841.92related to AP2 2 [more]
AT1G53910.34.6e-2842.70related to AP2 12 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 102..122
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 43..66
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..67
NoneNo IPR availablePANTHERPTHR31190:SF210EREBP TRANSCRIPTION FACTORcoord: 45..131
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 70..81
score: 70.45
coord: 92..108
score: 73.69
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 69..132
e-value: 3.0E-39
score: 146.4
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 70..119
e-value: 3.7E-15
score: 55.9
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 69..126
score: 23.74897
IPR001471AP2/ERF domainCDDcd00018AP2coord: 70..125
e-value: 1.15508E-33
score: 113.906
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 69..127
e-value: 6.1E-27
score: 95.6
IPR044808Ethylene-responsive transcription factorPANTHERPTHR31190DNA-BINDING DOMAINcoord: 45..131
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 69..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg025074.1Spg025074.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009873 ethylene-activated signaling pathway
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity