Spg022632 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg022632
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionDipeptide epimerase
Locationscaffold2: 3508704 .. 3511359 (+)
RNA-Seq ExpressionSpg022632
SyntenySpg022632
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAATTGCAGTTGGTACTGTTGTTCTTTGGTTCCTTCTGGCTTTCTTCTGAACAAAACGAAGCAAAGCGAAAATGACAAAGCCGGTTCTTTTCTGTCTCCTTCGCTGTCCAATTGTTGAACAGCGACGGAATTCAAAATTCCCAAATTCACCGTTCAAATCCCCTTCTGAACACTCTCATGAGTTCCACTGGGTTTTTGATGCAATTGAAATCCAATACAGCGCCCATATACTGTAAGTTGTTTGACGCTCGAAGGAAGAGGAAGACATCCACACCCATTTCGCGGCTGTGTGTGAAGATGATGGCGGCAGCGGCAGCGGCGGAGGCCAGAACGAGCTTCGGGTTCAAGAATTTGATGGAAACGTTCACTGTGAATGTGCAGAGAGCGGAAAATAGGGCACTGAATGTTCCGCTGGTCGAACCATTTACGATTGCATCTTCGAGGCTTGAAATGGTGGAGAATGTAGCGATAAGGATCGAATTGAGTGATGGGTGCGTCGGATGGGGCGAGGCCCCGATTTTGCCTTTTGTCACCGCGGAGGATCAGCCCACCGCCATGGCGAAGGCAGAGGAGGTTTGTGAGCTGTTGCGACAGAGACCGCCGATTACTTTGAGCTCGGCGATGTTGCAGATTAGCGAGACTCTTCCTGGGCATGAATTTGCTTCTGTGAGTAATCAATTAAGACACCGGATTTTAACTTTTGACTTCATTTTTCTTTTTTCGTTACTCAAATTGGTAATATAATGCATTTTGATGAAGAATTGCTTCCATAGAAGGATTTTGTTCCATGGCTTCGTGTTGTAATGCTTGGAATGCCCTGCCCTGCATTTTTAAGAACCAATCACTGAGTTTCTGAACCCATGTTTGTTTGATCACTGTATATCATCTACACTGTTGAAGGTTAGAGCAGGAGTTGAAATGGCATTGATTGATGCAGTTGCTAACAGCATTAACATACCTCTATGGAGGCTGTTTGGTGGAGTTTCAGACAGTATAACCACTGATATAACCGTAAGTTTCCTTTTATCTTGCAGCGAAAGCCATGTTTGAATCATCATGAACTGCAAACTCTTTTAGTTTTATCCAAATGAGCCTAGTAAATATAAGGATAAATGAAAATTCTAGTTATACCAATGAGAAGTAAGCCAAAAGAAAATGGTCACAAAACTACTCTCATCCATGTTATTTATGCAGATTCCTATTACTTCTGCTTCTAATGCTGCCAAACTGGCTGCAAAATATCGTGATCAAGGATTCAAGACTTTGAAGTTAAAGGTAGGAAAGGATCTGAAAGCGGATATTGAAGTTCTGCAGTGCATCAGAATGGTCCATCCTGATTGCGAATTTATCCTGGATGCTAATGAGGGATATGACACTGAGGAAGCTATTCAAGTTCTTGAAAAATTACATGGTAACGGATGAGTACTTCTGTAATTGGAATCACTTTTATTCTGCCTTGGTGCCTTCTGAAATATCTGTTTCCTTCTCACCCCTGCAATTTTTTAAATTTATTGTTTTTCTCCATGGTGGTTTCCCTAAAAATGGATTATTATTCATACAGAATTGGGGGTGACGCCTACTCTGTTTGAACAACCAGTTCATAGAGACAACTGGGAGGGTCTTGGAATGATTAGTCGCATCGCAAGAGATAAATACGGGGTGTCCGTTGCAGCTGATGAGAGTTGTCGGAGTGTAAATGATGTTAAAAGAATAGTGAAAGAGGATCTCGCAGATGTTATAAATATTAAGCTTGCCAAAGTTGGAGTTTTGGGAGCCATTGAAATTATTGAAGTAGCAAGAGCTTCAGGCTTAACTTTAATGATTGGTGGTATGGTAGAGACTCGACTCGCCATGGGCTTTTCTGGTCATCTTGCTGCTGGCCTTGGGTGTTTCAAGTATGCATTCTTTATGGATTATGCTCGTTGATTTTAACATTTCTCTATAAATCAATTGTTTTTTGTCCAATTGCAGATACATCGACTTAGATACACCACTTTTATTGTCAGAAGATCCTGTTCATGGGGGTTATGAAGGTATATATTGAACTTTGATTCATTAAAGTTTTAGACTTTCCTATAACTTACTTGGAAGTAGTTTGGTAATTCTGATATAATTGTAATGAATTTTGGCAAGTAGTTTCTGGTGCTGTCTACAAGTTCACAAATGCCAGAGGCCATGGTGGTTTCCTTCACTGGGATAACTTTGCATGGTAAGTTTTGAAGTTTCCTTGAAGATTTAGATATGGGCTTATCTGCTATTTCAGGAGCCACTGAGCTTCAATCCAATTTTACATGAACCTTGTTTGATTACATTAACAAACCCCTCTACTCAGGACTGATAATGATGAATATGTATGGTTTTTGTAGTTGATGATTACTTTCAACTGAATCTTCTACATCAATGGAACTCTCGCTCGCCATGATTTTCCCCGAACCGAAGTCTTTCCAAGTCCGAATTTTCGGGGCAAGTTTGGTAATTGAGATTCATGAATGTTCATCAGCACTCTAAGTTATTGATGTAAATCTTGTTTGTACCTATGGTTGATTCCTTGCTACACAAATAGTCATTTCAGCTGTCAGTGACTACAGTCATGGTCAAAATATCAAGAAAATGGAAGCATTTTAAAACTTCATTTGCTGTCTCGTTTAAT

mRNA sequence

ATGAGTTCCACTGGGTTTTTGATGCAATTGAAATCCAATACAGCGCCCATATACTGTAAGTTGTTTGACGCTCGAAGGAAGAGGAAGACATCCACACCCATTTCGCGGCTGTGTGTGAAGATGATGGCGGCAGCGGCAGCGGCGGAGGCCAGAACGAGCTTCGGGTTCAAGAATTTGATGGAAACGTTCACTGTGAATGTGCAGAGAGCGGAAAATAGGGCACTGAATGTTCCGCTGGTCGAACCATTTACGATTGCATCTTCGAGGCTTGAAATGGTGGAGAATGTAGCGATAAGGATCGAATTGAGTGATGGGTGCGTCGGATGGGGCGAGGCCCCGATTTTGCCTTTTGTCACCGCGGAGGATCAGCCCACCGCCATGGCGAAGGCAGAGGAGGTTTGTGAGCTGTTGCGACAGAGACCGCCGATTACTTTGAGCTCGGCGATGTTGCAGATTAGCGAGACTCTTCCTGGGCATGAATTTGCTTCTGTTAGAGCAGGAGTTGAAATGGCATTGATTGATGCAGTTGCTAACAGCATTAACATACCTCTATGGAGGCTGTTTGGTGGAGTTTCAGACAGTATAACCACTGATATAACCATTCCTATTACTTCTGCTTCTAATGCTGCCAAACTGGCTGCAAAATATCGTGATCAAGGATTCAAGACTTTGAAGTTAAAGGTAGGAAAGGATCTGAAAGCGGATATTGAAGTTCTGCAGTGCATCAGAATGGTCCATCCTGATTGCGAATTTATCCTGGATGCTAATGAGGGATATGACACTGAGGAAGCTATTCAAGTTCTTGAAAAATTACATGAATTGGGGGTGACGCCTACTCTGTTTGAACAACCAGTTCATAGAGACAACTGGGAGGGTCTTGGAATGATTAGTCGCATCGCAAGAGATAAATACGGGGTGTCCGTTGCAGCTGATGAGAGTTGTCGGAGTGTAAATGATGTTAAAAGAATAGTGAAAGAGGATCTCGCAGATGTTATAAATATTAAGCTTGCCAAAGTTGGAGTTTTGGGAGCCATTGAAATTATTGAAGTAGCAAGAGCTTCAGGCTTAACTTTAATGATTGGTGGTATGGTAGAGACTCGACTCGCCATGGGCTTTTCTGGTCATCTTGCTGCTGGCCTTGGGTGTTTCAAATACATCGACTTAGATACACCACTTTTATTGTCAGAAGATCCTGTTCATGGGGGTTATGAAGTAGTTTCTGGTGCTGTCTACAAGTTCACAAATGCCAGAGGCCATGGTGGTTTCCTTCACTGGGATAACTTTGCATGTTGA

Coding sequence (CDS)

ATGAGTTCCACTGGGTTTTTGATGCAATTGAAATCCAATACAGCGCCCATATACTGTAAGTTGTTTGACGCTCGAAGGAAGAGGAAGACATCCACACCCATTTCGCGGCTGTGTGTGAAGATGATGGCGGCAGCGGCAGCGGCGGAGGCCAGAACGAGCTTCGGGTTCAAGAATTTGATGGAAACGTTCACTGTGAATGTGCAGAGAGCGGAAAATAGGGCACTGAATGTTCCGCTGGTCGAACCATTTACGATTGCATCTTCGAGGCTTGAAATGGTGGAGAATGTAGCGATAAGGATCGAATTGAGTGATGGGTGCGTCGGATGGGGCGAGGCCCCGATTTTGCCTTTTGTCACCGCGGAGGATCAGCCCACCGCCATGGCGAAGGCAGAGGAGGTTTGTGAGCTGTTGCGACAGAGACCGCCGATTACTTTGAGCTCGGCGATGTTGCAGATTAGCGAGACTCTTCCTGGGCATGAATTTGCTTCTGTTAGAGCAGGAGTTGAAATGGCATTGATTGATGCAGTTGCTAACAGCATTAACATACCTCTATGGAGGCTGTTTGGTGGAGTTTCAGACAGTATAACCACTGATATAACCATTCCTATTACTTCTGCTTCTAATGCTGCCAAACTGGCTGCAAAATATCGTGATCAAGGATTCAAGACTTTGAAGTTAAAGGTAGGAAAGGATCTGAAAGCGGATATTGAAGTTCTGCAGTGCATCAGAATGGTCCATCCTGATTGCGAATTTATCCTGGATGCTAATGAGGGATATGACACTGAGGAAGCTATTCAAGTTCTTGAAAAATTACATGAATTGGGGGTGACGCCTACTCTGTTTGAACAACCAGTTCATAGAGACAACTGGGAGGGTCTTGGAATGATTAGTCGCATCGCAAGAGATAAATACGGGGTGTCCGTTGCAGCTGATGAGAGTTGTCGGAGTGTAAATGATGTTAAAAGAATAGTGAAAGAGGATCTCGCAGATGTTATAAATATTAAGCTTGCCAAAGTTGGAGTTTTGGGAGCCATTGAAATTATTGAAGTAGCAAGAGCTTCAGGCTTAACTTTAATGATTGGTGGTATGGTAGAGACTCGACTCGCCATGGGCTTTTCTGGTCATCTTGCTGCTGGCCTTGGGTGTTTCAAATACATCGACTTAGATACACCACTTTTATTGTCAGAAGATCCTGTTCATGGGGGTTATGAAGTAGTTTCTGGTGCTGTCTACAAGTTCACAAATGCCAGAGGCCATGGTGGTTTCCTTCACTGGGATAACTTTGCATGTTGA

Protein sequence

MSSTGFLMQLKSNTAPIYCKLFDARRKRKTSTPISRLCVKMMAAAAAAEARTSFGFKNLMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFVTAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVANSINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIEVLQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISRIARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTLMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNARGHGGFLHWDNFAC
Homology
BLAST of Spg022632 vs. NCBI nr
Match: XP_008453435.1 (PREDICTED: L-Ala-D/L-amino acid epimerase [Cucumis melo] >TYK28449.1 L-Ala-D/L-amino acid epimerase [Cucumis melo var. makuwa])

HSP 1 Score: 754.2 bits (1946), Expect = 6.1e-214
Identity = 387/431 (89.79%), Postives = 407/431 (94.43%), Query Frame = 0

Query: 1   MSSTGFLMQLKSNTAPIYCKLFDARRKRK--TSTPISRLCVKMMAAAAAAEARTSFGFKN 60
           M+S GF +QLK NTA IY  LFD+R  RK  TS PISRLCVK+MAAAA A++RTS GFKN
Sbjct: 1   MTSIGFFVQLKFNTASIYPLLFDSRSHRKGITSVPISRLCVKIMAAAAPADSRTSLGFKN 60

Query: 61  LMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFV 120
           LMETFTVNVQRAENRALNVPL+EPFTIASSRLEMVENVAIRIELS+GCVGWGEAPILPFV
Sbjct: 61  LMETFTVNVQRAENRALNVPLIEPFTIASSRLEMVENVAIRIELSNGCVGWGEAPILPFV 120

Query: 121 TAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVAN 180
           TAEDQPTAMAKAEEVCELLRQRPP TL  AM+QISETLPGHEFASVRAGVEMALIDAVAN
Sbjct: 121 TAEDQPTAMAKAEEVCELLRQRPPSTLGLAMMQISETLPGHEFASVRAGVEMALIDAVAN 180

Query: 181 SINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIEV 240
           SINIPLW+LFGG SDSITTDITIPITSAS+AAKLAAKYRDQGFKTLKLKVGKDLK+DIEV
Sbjct: 181 SINIPLWKLFGGASDSITTDITIPITSASHAAKLAAKYRDQGFKTLKLKVGKDLKSDIEV 240

Query: 241 LQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISR 300
           L+ IRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLG +SR
Sbjct: 241 LKSIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGNVSR 300

Query: 301 IARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTL 360
           +ARDKYGVSVAADESCR V+DVKRIV+ DLADV+NIKLAKVGVLGAIEIIEVAR SGL+L
Sbjct: 301 VARDKYGVSVAADESCRGVDDVKRIVEGDLADVVNIKLAKVGVLGAIEIIEVARGSGLSL 360

Query: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNARG 420
           MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE VSGAVYKFTN+RG
Sbjct: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE-VSGAVYKFTNSRG 420

Query: 421 HGGFLHWDNFA 430
           HGGFLHWDN A
Sbjct: 421 HGGFLHWDNIA 430

BLAST of Spg022632 vs. NCBI nr
Match: KAA0058096.1 (L-Ala-D/L-amino acid epimerase [Cucumis melo var. makuwa])

HSP 1 Score: 750.4 bits (1936), Expect = 8.8e-213
Identity = 386/431 (89.56%), Postives = 406/431 (94.20%), Query Frame = 0

Query: 1   MSSTGFLMQLKSNTAPIYCKLFDAR--RKRKTSTPISRLCVKMMAAAAAAEARTSFGFKN 60
           M+  GF +QLK NTA I   LFD+R  RK KTS PISRLCVK+MAAAA A++RTS GFKN
Sbjct: 1   MTFIGFFVQLKFNTASICPLLFDSRSHRKGKTSVPISRLCVKIMAAAAPADSRTSLGFKN 60

Query: 61  LMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFV 120
           LMETFTVNVQRAENRALNVPL+EPFTIASSRLEMVENVAIRIELS+GCVGWGEAPILPFV
Sbjct: 61  LMETFTVNVQRAENRALNVPLIEPFTIASSRLEMVENVAIRIELSNGCVGWGEAPILPFV 120

Query: 121 TAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVAN 180
           TAEDQPTAMAKAEEVCELLRQRPP TL  AM+QISETLPGHEFASVRAGVEMALIDAVAN
Sbjct: 121 TAEDQPTAMAKAEEVCELLRQRPPSTLGLAMMQISETLPGHEFASVRAGVEMALIDAVAN 180

Query: 181 SINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIEV 240
           SINIPLW+LFGG SDSITTDITIPITSAS+AAKLAAKYRDQGFKTLKLKVGKDLK+DIEV
Sbjct: 181 SINIPLWKLFGGASDSITTDITIPITSASHAAKLAAKYRDQGFKTLKLKVGKDLKSDIEV 240

Query: 241 LQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISR 300
           L+ IRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLG +SR
Sbjct: 241 LKSIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGNVSR 300

Query: 301 IARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTL 360
           +ARDKYGVSVAADESCR V+DVKRIV+ DLADV+NIKLAKVGVLGAIEIIEVAR SGL+L
Sbjct: 301 VARDKYGVSVAADESCRGVDDVKRIVEGDLADVVNIKLAKVGVLGAIEIIEVARGSGLSL 360

Query: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNARG 420
           MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE VSGAVYKFTN+RG
Sbjct: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE-VSGAVYKFTNSRG 420

Query: 421 HGGFLHWDNFA 430
           HGGFLHWDN A
Sbjct: 421 HGGFLHWDNIA 430

BLAST of Spg022632 vs. NCBI nr
Match: XP_004137322.1 (L-Ala-D/L-amino acid epimerase [Cucumis sativus] >KGN63844.2 hypothetical protein Csa_013780 [Cucumis sativus])

HSP 1 Score: 747.7 bits (1929), Expect = 5.7e-212
Identity = 386/432 (89.35%), Postives = 407/432 (94.21%), Query Frame = 0

Query: 1   MSSTGFLMQLKSNTAPIYCKLFDARR--KRKTSTP-ISRLCVKMMAAAAAAEARTSFGFK 60
           M+ST F +QLKSNTA +Y  LFD+R   K KTS P I RLCVK+MAAAA A++R S GFK
Sbjct: 1   MTSTAFFLQLKSNTASVYPLLFDSRSHWKGKTSVPIILRLCVKIMAAAAPADSRNSLGFK 60

Query: 61  NLMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPF 120
           NLMETFTVNVQRAENR LNVPL+EPFTIASSRLEMVENVAIRIELS+GCVGWGEAPILPF
Sbjct: 61  NLMETFTVNVQRAENRTLNVPLIEPFTIASSRLEMVENVAIRIELSNGCVGWGEAPILPF 120

Query: 121 VTAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVA 180
           VTAEDQ TAMAKAEEVCELLRQRPP TL SAM+QISETLPGHEFASVRAGVEMALIDAVA
Sbjct: 121 VTAEDQSTAMAKAEEVCELLRQRPPSTLGSAMMQISETLPGHEFASVRAGVEMALIDAVA 180

Query: 181 NSINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIE 240
           NSINIPLW+LFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLK+DIE
Sbjct: 181 NSINIPLWKLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKSDIE 240

Query: 241 VLQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMIS 300
           VL+ IRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLG +S
Sbjct: 241 VLKSIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGNVS 300

Query: 301 RIARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLT 360
           R+ARDKYGVSVAADESCRSV+DVKRIV+ DLADVINIKLAKVGVLGAIEIIEVAR SGL+
Sbjct: 301 RVARDKYGVSVAADESCRSVDDVKRIVEGDLADVINIKLAKVGVLGAIEIIEVARGSGLS 360

Query: 361 LMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNAR 420
           LMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE VSGAVYKFTN++
Sbjct: 361 LMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE-VSGAVYKFTNSQ 420

Query: 421 GHGGFLHWDNFA 430
           GHGG+LHWDN A
Sbjct: 421 GHGGYLHWDNIA 431

BLAST of Spg022632 vs. NCBI nr
Match: XP_022134566.1 (L-Ala-D/L-amino acid epimerase [Momordica charantia])

HSP 1 Score: 746.9 bits (1927), Expect = 9.8e-212
Identity = 389/433 (89.84%), Postives = 403/433 (93.07%), Query Frame = 0

Query: 1   MSSTGFLMQLKSNTAPIYCKLFDAR--RKRKTSTPISRLCVKMMAAAAAA--EARTSFGF 60
           MS  GF MQLKS TA IYC LF+AR  RK K S PI   CVK MAAAAAA  EARTSFGF
Sbjct: 1   MSFAGFSMQLKSYTASIYCPLFNARSQRKGKPSIPIFPQCVKQMAAAAAAPVEARTSFGF 60

Query: 61  KNLMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILP 120
            NLMETFTVNVQRAENR LNVPLV PFTIASSRLEMVENVAIRIELS+GCVGWGEAPILP
Sbjct: 61  NNLMETFTVNVQRAENRPLNVPLVAPFTIASSRLEMVENVAIRIELSNGCVGWGEAPILP 120

Query: 121 FVTAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAV 180
           FVTAEDQPTAMAKA+E CELL+QRPP TL SAM+QISE LPGHEFASVRAGVEMALIDAV
Sbjct: 121 FVTAEDQPTAMAKAKEACELLQQRPPTTLRSAMMQISEVLPGHEFASVRAGVEMALIDAV 180

Query: 181 ANSINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADI 240
           ANSINIPLWRLFGGVSDSITTDITIPITSASNAAKLA+KYR+QGFKTLKLKVGKDLKADI
Sbjct: 181 ANSINIPLWRLFGGVSDSITTDITIPITSASNAAKLASKYREQGFKTLKLKVGKDLKADI 240

Query: 241 EVLQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMI 300
           EVLQCIRMVHP+CEFILDANEGY TEEAIQVL KL+ELGVTPTLFEQPVHRDNWEGLG +
Sbjct: 241 EVLQCIRMVHPECEFILDANEGYGTEEAIQVLAKLYELGVTPTLFEQPVHRDNWEGLGQV 300

Query: 301 SRIARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGL 360
           SRIARDK+G+SVAADESCRS+ DVKRIV+EDLADVINIKLAKVGVLGAIEIIEVARASGL
Sbjct: 301 SRIARDKFGISVAADESCRSLTDVKRIVEEDLADVINIKLAKVGVLGAIEIIEVARASGL 360

Query: 361 TLMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNA 420
           TLMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE VSGAVYKFTNA
Sbjct: 361 TLMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE-VSGAVYKFTNA 420

Query: 421 RGHGGFLHWDNFA 430
           RGHGGFLHWDN A
Sbjct: 421 RGHGGFLHWDNIA 432

BLAST of Spg022632 vs. NCBI nr
Match: XP_038880333.1 (L-Ala-D/L-amino acid epimerase [Benincasa hispida])

HSP 1 Score: 745.3 bits (1923), Expect = 2.8e-211
Identity = 389/431 (90.26%), Postives = 401/431 (93.04%), Query Frame = 0

Query: 1   MSSTGFLMQLKSNTAPIYCKLFDAR--RKRKTSTPISRLCVKMMAAAAAAEARTSFGFKN 60
           MSS GF ++ KS+TA IY  LFD+R  RK  TS PIS LCVKMM    +AEARTS GFKN
Sbjct: 1   MSSIGFFVRSKSSTASIYSPLFDSRSHRKGSTSIPISPLCVKMM----SAEARTSLGFKN 60

Query: 61  LMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFV 120
            METFTVNVQRAENRALNVPL+EPFTIASSRLEMVENVAIRIELS+GCVGWGEAPILPFV
Sbjct: 61  FMETFTVNVQRAENRALNVPLIEPFTIASSRLEMVENVAIRIELSNGCVGWGEAPILPFV 120

Query: 121 TAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVAN 180
           TAEDQPTAMAKAEE CELLRQRPP TL SAMLQISE LPGHEFASVRAGVEMALIDAVAN
Sbjct: 121 TAEDQPTAMAKAEEACELLRQRPPSTLGSAMLQISEALPGHEFASVRAGVEMALIDAVAN 180

Query: 181 SINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIEV 240
           SINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKY DQGFKTLKLKVGKDLKADIEV
Sbjct: 181 SINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYCDQGFKTLKLKVGKDLKADIEV 240

Query: 241 LQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISR 300
           LQ IR+VHP+CEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLG +S 
Sbjct: 241 LQSIRLVHPECEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGHVSH 300

Query: 301 IARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTL 360
           IARDKYGVSVAADESCRSVNDVKRIV+ DLADVINIKLAKVGVLGAIEIIEVARASGLTL
Sbjct: 301 IARDKYGVSVAADESCRSVNDVKRIVEGDLADVINIKLAKVGVLGAIEIIEVARASGLTL 360

Query: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNARG 420
           MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE VSGAVYKFTN+RG
Sbjct: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE-VSGAVYKFTNSRG 420

Query: 421 HGGFLHWDNFA 430
           HGGFL WDN A
Sbjct: 421 HGGFLRWDNIA 426

BLAST of Spg022632 vs. ExPASy Swiss-Prot
Match: B9I2J6 (L-Ala-D/L-amino acid epimerase OS=Populus trichocarpa OX=3694 GN=POPTR_0012s05040g PE=3 SV=2)

HSP 1 Score: 580.1 bits (1494), Expect = 2.1e-164
Identity = 297/399 (74.44%), Postives = 337/399 (84.46%), Query Frame = 0

Query: 29  KTSTPISRLCVKMMAAAAAAEARTSFGFKNLMETFTVNVQRAENRALNVPLVEPFTIASS 88
           KT  PI +L      A +  +  ++F F++LMETF V+V+RAENR LNVPL+ PFTIASS
Sbjct: 22  KTPKPIRKLYATKAMAGSNPKQVSTFEFRDLMETFAVDVKRAENRPLNVPLIAPFTIASS 81

Query: 89  RLEMVENVAIRIELSDGCVGWGEAPILPFVTAEDQPTAMAKAEEVCELLRQRPPITLSSA 148
           RL+ VENVAIRIELSDGCVGWGEAPILPFVTAEDQ TAM KA E CELL+    + L   
Sbjct: 82  RLDKVENVAIRIELSDGCVGWGEAPILPFVTAEDQSTAMIKAREACELLKNSSSMKLGLV 141

Query: 149 MLQISETLPGHEFASVRAGVEMALIDAVANSINIPLWRLFGGVSDSITTDITIPITSASN 208
           + ++SE LPGHEFASVRAGVEMALIDAVA SIN+PLW LFGG SDSITTDITIPI S++ 
Sbjct: 142 LERVSEILPGHEFASVRAGVEMALIDAVAKSINVPLWILFGGASDSITTDITIPIVSSAE 201

Query: 209 AAKLAAKYRDQGFKTLKLKVGKDLKADIEVLQCIRMVHPDCEFILDANEGYDTEEAIQVL 268
           AA+LA+KYR QGF+TLKLKVGK+LK DIEVLQ IR VHPDC FILDANEGY  EEAI+VL
Sbjct: 202 AAELASKYRKQGFQTLKLKVGKNLKEDIEVLQAIRAVHPDCLFILDANEGYKPEEAIEVL 261

Query: 269 EKLHELGVTPTLFEQPVHRDNWEGLGMISRIARDKYGVSVAADESCRSVNDVKRIVKEDL 328
           E+LH++GVTP LFEQPVHRD+WEGLG ++ IA+ KYGVSVAADESCRS+ D KRI+K +L
Sbjct: 262 EELHKMGVTPILFEQPVHRDDWEGLGHVTHIAKGKYGVSVAADESCRSLVDAKRIIKGNL 321

Query: 329 ADVINIKLAKVGVLGAIEIIEVARASGLTLMIGGMVETRLAMGFSGHLAAGLGCFKYIDL 388
           ADVINIKLAKVGV+G +EIIE AR SGL LMIGGMVETRLAMGF+GHLAAG GCFK+IDL
Sbjct: 322 ADVINIKLAKVGVVGGLEIIEEARTSGLDLMIGGMVETRLAMGFAGHLAAGFGCFKFIDL 381

Query: 389 DTPLLLSEDPVHGGYEVVSGAVYKFTNARGHGGFLHWDN 428
           DTPLLLSEDPV  GYE VSGAVYKFT+A+GH GFL WDN
Sbjct: 382 DTPLLLSEDPVLEGYE-VSGAVYKFTDAQGHAGFLDWDN 419

BLAST of Spg022632 vs. ExPASy Swiss-Prot
Match: A9B055 (Aromatic dipeptide epimerase OS=Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95) OX=316274 GN=Haur_1114 PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 3.3e-61
Identity = 144/357 (40.34%), Postives = 203/357 (56.86%), Query Frame = 0

Query: 67  VQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFVTAEDQPTA 126
           +Q     A+N+PL EPF IAS    +  NV ++++L+DG +G GEA   P V+ E Q   
Sbjct: 5   IQAISAEAINLPLTEPFAIASGAQAVAANVLVKVQLADGTLGLGEAAPFPAVSGETQTGT 64

Query: 127 MAKAEEVCELL--RQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVANSINIPL 186
            A  E +   L           +AML  +E    HE A+ R G+EMA++DA+    ++PL
Sbjct: 65  SAAIERLQSHLLGADVRGWRKLAAMLDHAE----HEAAAARCGLEMAMLDALTRHYHMPL 124

Query: 187 WRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLK-VGKDLKADIEVLQCIR 246
              FGGVS  + TD+TI      +AA  A     +G K++K+K  G D+  D+  L+ I 
Sbjct: 125 HVFFGGVSKQLETDMTITAGDEVHAAASAKAILARGIKSIKVKTAGVDVAYDLARLRAIH 184

Query: 247 MVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISRIARDK 306
              P    I+D N GYD E A+          +   LFEQP+ R++W G+  ++     +
Sbjct: 185 QAAPTAPLIVDGNCGYDVERALAFCAACKAESIPMVLFEQPLPREDWAGMAQVTA----Q 244

Query: 307 YGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTLMIGGM 366
            G +VAADES RS +DV RI +E  A VINIKL K GV   +++I +A+A+GL LMIGGM
Sbjct: 245 SGFAVAADESARSAHDVLRIAREGTASVINIKLMKAGVAEGLKMIAIAQAAGLGLMIGGM 304

Query: 367 VETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNARGHG 421
           VE+ LAM FS +LAAG G F +IDLDTPL ++E P  GG+   +G   +  +  GHG
Sbjct: 305 VESILAMSFSANLAAGNGGFDFIDLDTPLFIAEHPFIGGF-AQTGGTLQLADVAGHG 352

BLAST of Spg022632 vs. ExPASy Swiss-Prot
Match: B5EFW2 (Hydrophobic dipeptide epimerase OS=Geobacter bemidjiensis (strain Bem / ATCC BAA-1014 / DSM 16622) OX=404380 GN=Gbem_2417 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 5.3e-51
Identity = 118/342 (34.50%), Postives = 180/342 (52.63%), Query Frame = 0

Query: 67  VQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFVTAEDQPTA 126
           +Q A    +  PL  PF IA+ + + +ENV +++   DG  G+GEA +   +T E  P  
Sbjct: 7   IQDAVASVIRAPLASPFRIATGQHDELENVFLKLTTRDGVSGYGEAAVASHITGETVPGT 66

Query: 127 MAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVANSINIPLWR 186
           +A  +     LR +      SA  Q +    G+   +  A +EMAL+D  +    IP +R
Sbjct: 67  LANLQNAAAALRGQTVDDAESACRQFAAAFAGNH--AGLAALEMALLDLSSRVRGIPFYR 126

Query: 187 LFGGVSD-----SITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIEVLQC 246
           LF  V+      + +TDIT+ I S   A   A ++  +GFK  K+K+G+D + D+  +  
Sbjct: 127 LFAPVAALEPRLAFSTDITVVIGSLDEARATAREFASRGFKAFKIKIGRDEQLDLARVLA 186

Query: 247 IRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISRIAR 306
           +  + PD + ILDAN G+     +  L++L   GV P L EQPV + +W+GL  I+  A 
Sbjct: 187 VHEIAPDSQIILDANMGFSAGSMLAFLDRLAAKGVRPVLLEQPVPKMDWDGLSEIT-AAL 246

Query: 307 DKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTLMIG 366
                 V ADES  S+ D +R +  +    IN+K  K G+L   EI  +A + G+ LM+G
Sbjct: 247 TGSETLVCADESVGSLADARRAIDSNAVSAINVKFMKSGILEGAEIARLAASRGIRLMLG 306

Query: 367 GMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGY 404
            M+E+ LA+  S H AAGL CF Y+D+DT   L  +  H  Y
Sbjct: 307 AMMESALAVTASAHFAAGLACFDYLDMDTTFFLKGELAHSPY 345

BLAST of Spg022632 vs. ExPASy Swiss-Prot
Match: Q9WXM1 (L-Ala-D/L-Glu epimerase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_0006 PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.2e-47
Identity = 119/323 (36.84%), Postives = 182/323 (56.35%), Query Frame = 0

Query: 81  EPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFVTAEDQPTAMAKAEEVCELLRQR 140
           +PF I  S      NV + I L  G  G+GEA     V  E     +A    V E++   
Sbjct: 18  KPFHITGSVSSESRNVEVEIVLESGVKGYGEASPSFRVNGERVEALLAIENAVREMI-TG 77

Query: 141 PPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVANSINIPLWRLFGGVSDSITTDIT 200
             +   + + +I++ L G  F S++A V+ A +DA++  +   +  L GG  D I TD T
Sbjct: 78  IDVRNYARIFEITDRLFG--FPSLKAAVQFATLDALSQELGTQVCYLLGGKRDEIETDKT 137

Query: 201 IPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIEVLQCIRMVHPDCEFILDANEGYD 260
           + I +  N  K A K  ++GF+ +K+KVG++LK DIE ++ I  V    ++I+DAN GY 
Sbjct: 138 VGIDTVENRVKEAKKIFEEGFRVIKIKVGENLKEDIEAVEEIAKVTRGAKYIVDANMGYT 197

Query: 261 TEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISRIARDKYGVSVAADESCRSVNDV 320
            +EA++    +++ G+   ++EQPV R++ EGL    +  R      VAADES R+  DV
Sbjct: 198 QKEAVEFARAVYQKGIDIAVYEQPVRREDIEGL----KFVRFHSPFPVAADESARTKFDV 257

Query: 321 KRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTLMIGGMVETRLAMGFSGHLAAGL 380
            R+VKE+  D +NIKL K G+  A+ I+E+A +SGL LMIG M E+ L +  S H A G 
Sbjct: 258 MRLVKEEAVDYVNIKLMKSGISDALAIVEIAESSGLKLMIGCMGESSLGINQSVHFALGT 317

Query: 381 GCFKYIDLDTPLLLSEDPVHGGY 404
           G F++ DLD+ L+L E+   G +
Sbjct: 318 GAFEFHDLDSHLMLKEEVFRGKF 333

BLAST of Spg022632 vs. ExPASy Swiss-Prot
Match: O34508 (L-Ala-D/L-Glu epimerase OS=Bacillus subtilis (strain 168) OX=224308 GN=ykfB PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 4.3e-45
Identity = 124/360 (34.44%), Postives = 183/360 (50.83%), Query Frame = 0

Query: 65  VNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFVTAEDQP 124
           + + R E   + VPL +PF  A   +   E+V +RI    G VGWGEAP    +T +   
Sbjct: 1   MKIIRIETSRIAVPLTKPFKTALRTVYTAESVIVRITYDSGAVGWGEAPPTLVITGDSMD 60

Query: 125 TAMAKAEEVCELLRQRPPITLSSAML-QISETLPGHEFASVRAGVEMALIDAVANSINIP 184
           +  +    V +       +    A+L  I   L G+   S +A VEMAL D  A    +P
Sbjct: 61  SIESAIHHVLKPALLGKSLAGYEAILHDIQHLLTGN--MSAKAAVEMALYDGWAQMCGLP 120

Query: 185 LWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGK-DLKADIEVLQCI 244
           L+++ GG  D++ TD T+ + S    A  A  Y  QGF+TLK+KVGK D+  DI  +Q I
Sbjct: 121 LYQMLGGYRDTLETDYTVSVNSPEEMAADAENYLKQGFQTLKIKVGKDDIATDIARIQEI 180

Query: 245 R-MVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISRIAR 304
           R  V    +  LDAN+G+  +EA+  + K+ + G+   L EQPVH+D+  GL  ++    
Sbjct: 181 RKRVGSAVKLRLDANQGWRPKEAVTAIRKMEDAGLGIELVEQPVHKDDLAGLKKVT---- 240

Query: 305 DKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKV-GVLGAIEIIEVARASGLTLMI 364
           D     + ADES  +      +++   AD+INIKL K  G+ GA +I  +A A G+  M+
Sbjct: 241 DATDTPIMADESVFTPRQAFEVLQTRSADLINIKLMKAGGISGAEKINAMAEACGVECMV 300

Query: 365 GGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNARGHG 421
           G M+ET+L +  + H AA        D D PL+L  D  +GG    SG+        G G
Sbjct: 301 GSMIETKLGITAAAHFAASKRNITRFDFDAPLMLKTDVFNGGI-TYSGSTISMPGKPGLG 353

BLAST of Spg022632 vs. ExPASy TrEMBL
Match: A0A5D3DYK1 (Dipeptide epimerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00810 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 3.0e-214
Identity = 387/431 (89.79%), Postives = 407/431 (94.43%), Query Frame = 0

Query: 1   MSSTGFLMQLKSNTAPIYCKLFDARRKRK--TSTPISRLCVKMMAAAAAAEARTSFGFKN 60
           M+S GF +QLK NTA IY  LFD+R  RK  TS PISRLCVK+MAAAA A++RTS GFKN
Sbjct: 1   MTSIGFFVQLKFNTASIYPLLFDSRSHRKGITSVPISRLCVKIMAAAAPADSRTSLGFKN 60

Query: 61  LMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFV 120
           LMETFTVNVQRAENRALNVPL+EPFTIASSRLEMVENVAIRIELS+GCVGWGEAPILPFV
Sbjct: 61  LMETFTVNVQRAENRALNVPLIEPFTIASSRLEMVENVAIRIELSNGCVGWGEAPILPFV 120

Query: 121 TAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVAN 180
           TAEDQPTAMAKAEEVCELLRQRPP TL  AM+QISETLPGHEFASVRAGVEMALIDAVAN
Sbjct: 121 TAEDQPTAMAKAEEVCELLRQRPPSTLGLAMMQISETLPGHEFASVRAGVEMALIDAVAN 180

Query: 181 SINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIEV 240
           SINIPLW+LFGG SDSITTDITIPITSAS+AAKLAAKYRDQGFKTLKLKVGKDLK+DIEV
Sbjct: 181 SINIPLWKLFGGASDSITTDITIPITSASHAAKLAAKYRDQGFKTLKLKVGKDLKSDIEV 240

Query: 241 LQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISR 300
           L+ IRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLG +SR
Sbjct: 241 LKSIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGNVSR 300

Query: 301 IARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTL 360
           +ARDKYGVSVAADESCR V+DVKRIV+ DLADV+NIKLAKVGVLGAIEIIEVAR SGL+L
Sbjct: 301 VARDKYGVSVAADESCRGVDDVKRIVEGDLADVVNIKLAKVGVLGAIEIIEVARGSGLSL 360

Query: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNARG 420
           MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE VSGAVYKFTN+RG
Sbjct: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE-VSGAVYKFTNSRG 420

Query: 421 HGGFLHWDNFA 430
           HGGFLHWDN A
Sbjct: 421 HGGFLHWDNIA 430

BLAST of Spg022632 vs. ExPASy TrEMBL
Match: A0A1S3BWB1 (Dipeptide epimerase OS=Cucumis melo OX=3656 GN=LOC103494142 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 3.0e-214
Identity = 387/431 (89.79%), Postives = 407/431 (94.43%), Query Frame = 0

Query: 1   MSSTGFLMQLKSNTAPIYCKLFDARRKRK--TSTPISRLCVKMMAAAAAAEARTSFGFKN 60
           M+S GF +QLK NTA IY  LFD+R  RK  TS PISRLCVK+MAAAA A++RTS GFKN
Sbjct: 1   MTSIGFFVQLKFNTASIYPLLFDSRSHRKGITSVPISRLCVKIMAAAAPADSRTSLGFKN 60

Query: 61  LMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFV 120
           LMETFTVNVQRAENRALNVPL+EPFTIASSRLEMVENVAIRIELS+GCVGWGEAPILPFV
Sbjct: 61  LMETFTVNVQRAENRALNVPLIEPFTIASSRLEMVENVAIRIELSNGCVGWGEAPILPFV 120

Query: 121 TAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVAN 180
           TAEDQPTAMAKAEEVCELLRQRPP TL  AM+QISETLPGHEFASVRAGVEMALIDAVAN
Sbjct: 121 TAEDQPTAMAKAEEVCELLRQRPPSTLGLAMMQISETLPGHEFASVRAGVEMALIDAVAN 180

Query: 181 SINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIEV 240
           SINIPLW+LFGG SDSITTDITIPITSAS+AAKLAAKYRDQGFKTLKLKVGKDLK+DIEV
Sbjct: 181 SINIPLWKLFGGASDSITTDITIPITSASHAAKLAAKYRDQGFKTLKLKVGKDLKSDIEV 240

Query: 241 LQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISR 300
           L+ IRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLG +SR
Sbjct: 241 LKSIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGNVSR 300

Query: 301 IARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTL 360
           +ARDKYGVSVAADESCR V+DVKRIV+ DLADV+NIKLAKVGVLGAIEIIEVAR SGL+L
Sbjct: 301 VARDKYGVSVAADESCRGVDDVKRIVEGDLADVVNIKLAKVGVLGAIEIIEVARGSGLSL 360

Query: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNARG 420
           MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE VSGAVYKFTN+RG
Sbjct: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE-VSGAVYKFTNSRG 420

Query: 421 HGGFLHWDNFA 430
           HGGFLHWDN A
Sbjct: 421 HGGFLHWDNIA 430

BLAST of Spg022632 vs. ExPASy TrEMBL
Match: A0A5A7UVA5 (Dipeptide epimerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004040 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.3e-213
Identity = 386/431 (89.56%), Postives = 406/431 (94.20%), Query Frame = 0

Query: 1   MSSTGFLMQLKSNTAPIYCKLFDAR--RKRKTSTPISRLCVKMMAAAAAAEARTSFGFKN 60
           M+  GF +QLK NTA I   LFD+R  RK KTS PISRLCVK+MAAAA A++RTS GFKN
Sbjct: 1   MTFIGFFVQLKFNTASICPLLFDSRSHRKGKTSVPISRLCVKIMAAAAPADSRTSLGFKN 60

Query: 61  LMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFV 120
           LMETFTVNVQRAENRALNVPL+EPFTIASSRLEMVENVAIRIELS+GCVGWGEAPILPFV
Sbjct: 61  LMETFTVNVQRAENRALNVPLIEPFTIASSRLEMVENVAIRIELSNGCVGWGEAPILPFV 120

Query: 121 TAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVAN 180
           TAEDQPTAMAKAEEVCELLRQRPP TL  AM+QISETLPGHEFASVRAGVEMALIDAVAN
Sbjct: 121 TAEDQPTAMAKAEEVCELLRQRPPSTLGLAMMQISETLPGHEFASVRAGVEMALIDAVAN 180

Query: 181 SINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIEV 240
           SINIPLW+LFGG SDSITTDITIPITSAS+AAKLAAKYRDQGFKTLKLKVGKDLK+DIEV
Sbjct: 181 SINIPLWKLFGGASDSITTDITIPITSASHAAKLAAKYRDQGFKTLKLKVGKDLKSDIEV 240

Query: 241 LQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISR 300
           L+ IRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLG +SR
Sbjct: 241 LKSIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGNVSR 300

Query: 301 IARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTL 360
           +ARDKYGVSVAADESCR V+DVKRIV+ DLADV+NIKLAKVGVLGAIEIIEVAR SGL+L
Sbjct: 301 VARDKYGVSVAADESCRGVDDVKRIVEGDLADVVNIKLAKVGVLGAIEIIEVARGSGLSL 360

Query: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNARG 420
           MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE VSGAVYKFTN+RG
Sbjct: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE-VSGAVYKFTNSRG 420

Query: 421 HGGFLHWDNFA 430
           HGGFLHWDN A
Sbjct: 421 HGGFLHWDNIA 430

BLAST of Spg022632 vs. ExPASy TrEMBL
Match: A0A6J1BY55 (Dipeptide epimerase OS=Momordica charantia OX=3673 GN=LOC111006801 PE=3 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 4.7e-212
Identity = 389/433 (89.84%), Postives = 403/433 (93.07%), Query Frame = 0

Query: 1   MSSTGFLMQLKSNTAPIYCKLFDAR--RKRKTSTPISRLCVKMMAAAAAA--EARTSFGF 60
           MS  GF MQLKS TA IYC LF+AR  RK K S PI   CVK MAAAAAA  EARTSFGF
Sbjct: 1   MSFAGFSMQLKSYTASIYCPLFNARSQRKGKPSIPIFPQCVKQMAAAAAAPVEARTSFGF 60

Query: 61  KNLMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILP 120
            NLMETFTVNVQRAENR LNVPLV PFTIASSRLEMVENVAIRIELS+GCVGWGEAPILP
Sbjct: 61  NNLMETFTVNVQRAENRPLNVPLVAPFTIASSRLEMVENVAIRIELSNGCVGWGEAPILP 120

Query: 121 FVTAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAV 180
           FVTAEDQPTAMAKA+E CELL+QRPP TL SAM+QISE LPGHEFASVRAGVEMALIDAV
Sbjct: 121 FVTAEDQPTAMAKAKEACELLQQRPPTTLRSAMMQISEVLPGHEFASVRAGVEMALIDAV 180

Query: 181 ANSINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADI 240
           ANSINIPLWRLFGGVSDSITTDITIPITSASNAAKLA+KYR+QGFKTLKLKVGKDLKADI
Sbjct: 181 ANSINIPLWRLFGGVSDSITTDITIPITSASNAAKLASKYREQGFKTLKLKVGKDLKADI 240

Query: 241 EVLQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMI 300
           EVLQCIRMVHP+CEFILDANEGY TEEAIQVL KL+ELGVTPTLFEQPVHRDNWEGLG +
Sbjct: 241 EVLQCIRMVHPECEFILDANEGYGTEEAIQVLAKLYELGVTPTLFEQPVHRDNWEGLGQV 300

Query: 301 SRIARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGL 360
           SRIARDK+G+SVAADESCRS+ DVKRIV+EDLADVINIKLAKVGVLGAIEIIEVARASGL
Sbjct: 301 SRIARDKFGISVAADESCRSLTDVKRIVEEDLADVINIKLAKVGVLGAIEIIEVARASGL 360

Query: 361 TLMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNA 420
           TLMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE VSGAVYKFTNA
Sbjct: 361 TLMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE-VSGAVYKFTNA 420

Query: 421 RGHGGFLHWDNFA 430
           RGHGGFLHWDN A
Sbjct: 421 RGHGGFLHWDNIA 432

BLAST of Spg022632 vs. ExPASy TrEMBL
Match: A0A6J1EY69 (Dipeptide epimerase OS=Cucurbita moschata OX=3662 GN=LOC111439853 PE=3 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 4.9e-209
Identity = 381/431 (88.40%), Postives = 401/431 (93.04%), Query Frame = 0

Query: 1   MSSTGFLMQLKSNTAPIYCKLFDAR--RKRKTSTPISRLCVKMMAAAAAAEARTSFGFKN 60
           MSSTGF M L+S+TA  YC LFD R  RK K+S PISRLCVKM AA    E+  SFGFKN
Sbjct: 1   MSSTGFFMPLESDTASRYCPLFDFRSNRKEKSSIPISRLCVKMKAATVPFESGMSFGFKN 60

Query: 61  LMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPILPFV 120
           LMETFTVNVQRAE+RALNVPL EPFTIASSRLEMVENVAIRIELS+GCVGWGEAPILPFV
Sbjct: 61  LMETFTVNVQRAESRALNVPLFEPFTIASSRLEMVENVAIRIELSNGCVGWGEAPILPFV 120

Query: 121 TAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDAVAN 180
           TAEDQPTA+ KA EV ELL+QRPP  L SAM+QI ETLPGHEFASVRAGVEMALIDAVAN
Sbjct: 121 TAEDQPTAITKAGEVGELLQQRPPCNLGSAMMQIGETLPGHEFASVRAGVEMALIDAVAN 180

Query: 181 SINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKADIEV 240
           SINIPLWRLFGGVS+SITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDL+ADIEV
Sbjct: 181 SINIPLWRLFGGVSNSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLRADIEV 240

Query: 241 LQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGMISR 300
           LQ IR VH DCEFILDANEGYDTEEAIQVL+KLHELGVTPTLFEQPVHRDNWEGLG++SR
Sbjct: 241 LQRIRTVHSDCEFILDANEGYDTEEAIQVLDKLHELGVTPTLFEQPVHRDNWEGLGIVSR 300

Query: 301 IARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASGLTL 360
           IARDKYGVSVAADESCRS++DVKRIV+EDLADVINIKLAKVGVLGAIEIIEVARA+GL+L
Sbjct: 301 IARDKYGVSVAADESCRSLSDVKRIVEEDLADVINIKLAKVGVLGAIEIIEVARAAGLSL 360

Query: 361 MIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTNARG 420
           MIGGM+ETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE VSGAVYKFTNARG
Sbjct: 361 MIGGMIETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYE-VSGAVYKFTNARG 420

Query: 421 HGGFLHWDNFA 430
           HGGFLHWDN A
Sbjct: 421 HGGFLHWDNLA 430

BLAST of Spg022632 vs. TAIR 10
Match: AT3G18270.1 (cytochrome P450, family 77, subfamily A, polypeptide 5 pseudogene )

HSP 1 Score: 516.5 bits (1329), Expect = 2.0e-146
Identity = 252/374 (67.38%), Postives = 309/374 (82.62%), Query Frame = 0

Query: 56  FKNLMETFTVNVQRAENRALNVPLVEPFTIASSRLEMVENVAIRIELSDGCVGWGEAPIL 115
           FK L E FTV V +AENR LNV L+ PFTIASSRL+ V NVAIRIEL+DG VGWGEAPIL
Sbjct: 37  FKTLTENFTVRVLKAENRELNVALLSPFTIASSRLDSVSNVAIRIELNDGFVGWGEAPIL 96

Query: 116 PFVTAEDQPTAMAKAEEVCELLRQRPPITLSSAMLQISETLPGHEFASVRAGVEMALIDA 175
           P VTAEDQ  AM KA E  E LR+ P + L + + +I   LPGH+FASVRAG+EMA+IDA
Sbjct: 97  PSVTAEDQIMAMVKAREASEFLRELPEMKLGNVLQEIGRFLPGHQFASVRAGMEMAMIDA 156

Query: 176 VANSINIPLWRLFGGVSDSITTDITIPITSASNAAKLAAKYRDQGFKTLKLKVGKDLKAD 235
            A S+ +PLW+LFGG S +ITTDITIPI S + A+ LA+KYR +GF+TLKLKVGK+LKAD
Sbjct: 157 AAKSVRVPLWKLFGGASSTITTDITIPIVSPAEASVLASKYRKRGFETLKLKVGKNLKAD 216

Query: 236 IEVLQCIRMVHPDCEFILDANEGYDTEEAIQVLEKLHELGVTPTLFEQPVHRDNWEGLGM 295
           IEVLQ IR VHP C FILDANEGY TEEA++VLE LHE+ VTP LFEQPVHRDNWEGL  
Sbjct: 217 IEVLQAIRAVHPTCSFILDANEGYQTEEAVKVLETLHEMKVTPVLFEQPVHRDNWEGLSH 276

Query: 296 ISRIARDKYGVSVAADESCRSVNDVKRIVKEDLADVINIKLAKVGVLGAIEIIEVARASG 355
           ++R A++++GVS+AADESCR + D+K+I++ ++ DV+NIKLAK G+L ++E+IE+AR+SG
Sbjct: 277 VTRTAKNRFGVSIAADESCRGLTDLKKIIEGNIVDVVNIKLAKTGILESLEVIELARSSG 336

Query: 356 LTLMIGGMVETRLAMGFSGHLAAGLGCFKYIDLDTPLLLSEDPVHGGYEVVSGAVYKFTN 415
           + LMIGGMVETRLAMGFSGHLAAGLGCF++IDLDTPLLL++DPV GGY+   GAVY+F +
Sbjct: 337 IELMIGGMVETRLAMGFSGHLAAGLGCFRFIDLDTPLLLADDPVQGGYK-ACGAVYEFKD 396

Query: 416 ARGHGGFLHWDNFA 430
             GHGG+L W++ A
Sbjct: 397 EGGHGGYLQWNDVA 409

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008453435.16.1e-21489.79PREDICTED: L-Ala-D/L-amino acid epimerase [Cucumis melo] >TYK28449.1 L-Ala-D/L-a... [more]
KAA0058096.18.8e-21389.56L-Ala-D/L-amino acid epimerase [Cucumis melo var. makuwa][more]
XP_004137322.15.7e-21289.35L-Ala-D/L-amino acid epimerase [Cucumis sativus] >KGN63844.2 hypothetical protei... [more]
XP_022134566.19.8e-21289.84L-Ala-D/L-amino acid epimerase [Momordica charantia][more]
XP_038880333.12.8e-21190.26L-Ala-D/L-amino acid epimerase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
B9I2J62.1e-16474.44L-Ala-D/L-amino acid epimerase OS=Populus trichocarpa OX=3694 GN=POPTR_0012s0504... [more]
A9B0553.3e-6140.34Aromatic dipeptide epimerase OS=Herpetosiphon aurantiacus (strain ATCC 23779 / D... [more]
B5EFW25.3e-5134.50Hydrophobic dipeptide epimerase OS=Geobacter bemidjiensis (strain Bem / ATCC BAA... [more]
Q9WXM11.2e-4736.84L-Ala-D/L-Glu epimerase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / J... [more]
O345084.3e-4534.44L-Ala-D/L-Glu epimerase OS=Bacillus subtilis (strain 168) OX=224308 GN=ykfB PE=1... [more]
Match NameE-valueIdentityDescription
A0A5D3DYK13.0e-21489.79Dipeptide epimerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G... [more]
A0A1S3BWB13.0e-21489.79Dipeptide epimerase OS=Cucumis melo OX=3656 GN=LOC103494142 PE=3 SV=1[more]
A0A5A7UVA54.3e-21389.56Dipeptide epimerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G... [more]
A0A6J1BY554.7e-21289.84Dipeptide epimerase OS=Momordica charantia OX=3673 GN=LOC111006801 PE=3 SV=1[more]
A0A6J1EY694.9e-20988.40Dipeptide epimerase OS=Cucurbita moschata OX=3662 GN=LOC111439853 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G18270.12.0e-14667.38cytochrome P450, family 77, subfamily A, polypeptide 5 pseudogene [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013342Mandelate racemase/muconate lactonizing enzyme, C-terminalSMARTSM00922MR_MLE_2coord: 206..300
e-value: 2.6E-15
score: 66.9
IPR036849Enolase-like, C-terminal domain superfamilyGENE3D3.20.20.120coord: 181..420
e-value: 8.6E-93
score: 313.0
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILY51604Enolase C-terminal domain-likecoord: 189..424
IPR029017Enolase-like, N-terminalGENE3D3.30.390.10coord: 74..180
e-value: 8.6E-93
score: 313.0
IPR029017Enolase-like, N-terminalSUPERFAMILY54826Enolase N-terminal domain-likecoord: 66..190
IPR029065Enolase C-terminal domain-likePFAMPF13378MR_MLE_Ccoord: 211..420
e-value: 5.6E-35
score: 120.9
IPR013341Mandelate racemase/muconate lactonizing enzyme, N-terminal domainPFAMPF02746MR_MLE_Ncoord: 77..189
e-value: 1.6E-10
score: 41.2
NoneNo IPR availablePIRSRPIRSR634603-3PIRSR634603-3coord: 67..422
e-value: 9.0E-90
score: 299.0
NoneNo IPR availableSFLDSFLDF00009o-succinylbenzoate_synthasecoord: 70..399
e-value: 0.0
score: 152.0
NoneNo IPR availableSFLDSFLDS00001Enolasecoord: 70..399
e-value: 0.0
score: 152.0
NoneNo IPR availablePIRSRPIRSR634603-1PIRSR634603-1coord: 67..422
e-value: 9.0E-90
score: 299.0
NoneNo IPR availablePANTHERPTHR48073O-SUCCINYLBENZOATE SYNTHASE-RELATEDcoord: 19..430
NoneNo IPR availablePANTHERPTHR48073:SF4DIPEPTIDE EPIMERASEcoord: 19..430
IPR034603Dipeptide epimeraseCDDcd03319L-Ala-DL-Glu_epimerasecoord: 69..394
e-value: 2.92189E-106
score: 315.282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg022632.1Spg022632.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016829 lyase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016855 racemase and epimerase activity, acting on amino acids and derivatives