Spg010035 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg010035
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Ubiquitin-conjugating enzyme E2 C
Location	scaffold7: 6138965 .. 6141383 (-)
RNA-Seq Expression	Spg010035
Synteny	Spg010035

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTATTATTAACTTCCTTTCACAGCATAAAAGAAATAGTGAAAAGCCTTCGTCTTCATCCACCTTCTCCACTGACCCAAGAATTTCCTCGAGAAAAACAGAGAAAGAAACTGAAATTTTGGTGATTTTCACTCGATTTCGTCATCGAATCGAAGCATTTCTCGTGGATTTTGAGTCTAAGACGGTGGTATTGTGCATTTGATTGTCTGTTTGGTTCGTAGTTGGATCTAATTGGAGAGGATTCTTTGTGTTATTGATCGTTGTGGATATGGAGGTGAGACCCAATCCGACGGCCGACAACTCGTCGATCTCGCAGCATCATCTCACCAGTTCTCCATTGAAGCAGCCTGCCGCTTCTTCTAATCCTATCGACTCCACCGCTGTTGCGCAGAGGTGAAACTTCTTGTCGATCGTTCTTTTTGGTTTGTTGATTGTTTTGTTCCCGAGATTGTGGAGAATGTAAAGAATTGGATAAGAACGGAAAGAAAATGATGGAAAGTATTGAGGAATGTATGATTTGATGTTGCTTTCTTGTGTTTATAGTAAATCTGCTATGTTGATTGTTGGCTGCTTTTCCATGTTTTGGTTACCAAATTTGTAGAAGGCTTCAGATTCAAGCATTCAACGAATTCTTATTGATTGTGATTGTTGTTATGGGATTTTGACATTTTGCTTAATTTGGCTGCAGGTTGCAAAAGGAATTGATGTCTCTGATGGTATGTTTCCCAATTTCATTTTAGTTTTACATTTCTCATGGTGTTTCTTTGATTCTTGAATTTTGGTATTTCGTGGTCCTATTGAGTGGGTATTGATGGCATGTAGTTGGTTGAAACAGTGAGAATAAGCAAATTTTGGGTTTAATGATAGATCTTCATTTGGAAAATGTGTAATTTTTACTGCTCTTACCTTTTGTTGTAGAACCATGGTCCCATCCCATCCACTCCCTGGGACAAGTGTAGCTTATTTGAACCTTGACATTTTGTTTTGCAGCCTCTATGCTTCTTAATATCTATCATGTATGGATTTCAAATTTGCATTTCTTTTGTAATTATGGACATCTGTTAATTGGATTTTTTTGTATGTACCGTCGTAGTCTCATTCTTTTGGATTGGAGTCCATCCGGTAGGTAGTTTGAGGACTCTTTTTGTGAACCTTGTTTTTTAATATGCCCTTGTACATTCTTTCTTTTTTTCTTAATGAAAGCTTGGTTTCTTACCAGAAAAACTTTGCATTTCTTTTGTTGTAGAGCCATGATCATTAGGTGATTGCTGTTCTTGGATTTCAACTTTGCATTTCTTTGTCTTGTATCATGCTGCATGTTTGTTTATATGACATCTTGTTATGCAATAATAATCCGTGTTATCTTTACAACTGGAATTTCAAAACAATGAAATGATTGGAAAGGGTTAGGAACTAGGTTCGTTGTCTGCCTGCCTTCTGGATAGTTCATTAACCATATTTGTTTGCTGGTGCTCTCTACTATAGATGAGTGGAGGTGATCTTGGTGTATCAGCTTTTCCAGAAGGTGAGAGCATTTTCACATGGATTGGCACAATTGAGGGTGGGAAAGGAACTGCATATGAGGGTTTGTCTTACAAACTCTCTTTACATTTTCCCATGGATTACCCTTTCAAGCCACCCCTTGTTAAGTTTGAGACGATGTGCTTCCATCCAAATGTTGATCAATATGGCAACATTTGTCTCGACATTCTCCAGGTATCAATTAAATCTGCAGGGGTGTTTTTGCTTTTATGTTGTGGATGGTTATTGTACAAGATGGGAAAAATAACTATGATCCACATTTGGAAAAACTTTTTGATGTTTTTCTTTGTTGTTTATTATTGAAGTTTGTTAGGGCATATAAAGCACCACAACGTTCGTTTAGTTGATTTTTTTGAATCTCCTCCTGATATTGATGTAACTTCCTTTTCAATAGGACAAGTGGTCTTCAGCATATGATTGCAGAACCATTCTTTTGTCTATTCAAAGCCTATTGGGAGGTCTGCTCATTTCTGCTTGCATATTTAGCAATCTTCTAATCTCGATCTTCTCACCCTTATGAGGTTGTGTTTACTTGCAGAGCCCAACCCAGAAAGTCCTCTCAACAGCTATGCTGCTGCTCTGTGGAATAACAAGGAAGGTTAGACTCGACTCGACTCCCTCTCTCTCTCTCTCTCTCGCCTCCCTCTGCCCCCAATGACTGAAAAGTTCTTACTTCATCTGACACTAATAAATTATAAGCTTCGACATCGTCCTTTGCATTGAATTTGTTTCAAGGGCTGACTTCTCTTGCTCTTCTCATTATAAACATGAAGTAATGAAGGTATAAAGCTAAGCTGATTATTTGTTTTCTTGTTGTTCAGATTACAGAAAGATGGTCCACAAACAGTATTTTTCTGGCGCAGTCTTAGACGGCTGA

mRNA sequence

ATGGAGGTGAGACCCAATCCGACGGCCGACAACTCGTCGATCTCGCAGCATCATCTCACCAGTTCTCCATTGAAGCAGCCTGCCGCTTCTTCTAATCCTATCGACTCCACCGCTGTTGCGCAGAGGTTGCAAAAGGAATTGATGTCTCTGATGATGAGTGGAGGTGATCTTGGTGTATCAGCTTTTCCAGAAGGTGAGAGCATTTTCACATGGATTGGCACAATTGAGGGTGGGAAAGGAACTGCATATGAGGGTTTGTCTTACAAACTCTCTTTACATTTTCCCATGGATTACCCTTTCAAGCCACCCCTTGTTAAGTTTGAGACGATGTGCTTCCATCCAAATGTTGATCAATATGGCAACATTTGTCTCGACATTCTCCAGGACAAGTGGTCTTCAGCATATGATTGCAGAACCATTCTTTTGTCTATTCAAAGCCTATTGGGAGAGCCCAACCCAGAAAGTCCTCTCAACAGCTATGCTGCTGCTCTGTGGAATAACAAGGAAGATTACAGAAAGATGGTCCACAAACAGTATTTTTCTGGCGCAGTCTTAGACGGCTGA

Coding sequence (CDS)

ATGGAGGTGAGACCCAATCCGACGGCCGACAACTCGTCGATCTCGCAGCATCATCTCACCAGTTCTCCATTGAAGCAGCCTGCCGCTTCTTCTAATCCTATCGACTCCACCGCTGTTGCGCAGAGGTTGCAAAAGGAATTGATGTCTCTGATGATGAGTGGAGGTGATCTTGGTGTATCAGCTTTTCCAGAAGGTGAGAGCATTTTCACATGGATTGGCACAATTGAGGGTGGGAAAGGAACTGCATATGAGGGTTTGTCTTACAAACTCTCTTTACATTTTCCCATGGATTACCCTTTCAAGCCACCCCTTGTTAAGTTTGAGACGATGTGCTTCCATCCAAATGTTGATCAATATGGCAACATTTGTCTCGACATTCTCCAGGACAAGTGGTCTTCAGCATATGATTGCAGAACCATTCTTTTGTCTATTCAAAGCCTATTGGGAGAGCCCAACCCAGAAAGTCCTCTCAACAGCTATGCTGCTGCTCTGTGGAATAACAAGGAAGATTACAGAAAGATGGTCCACAAACAGTATTTTTCTGGCGCAGTCTTAGACGGCTGA

Protein sequence

MEVRPNPTADNSSISQHHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQYFSGAVLDG

Homology

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BLAST of Spg010035 vs. NCBI nr
Match: XP_022138984.1 (probable ubiquitin-conjugating enzyme E2 C [Momordica charantia])

HSP 1 Score: 375.9 bits (964), Expect = 2.0e-100
Identity = 181/187 (96.79%), Postives = 184/187 (98.40%), Query Frame = 0


Query: 1   MEVRPNPTADNSSISQHHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVS 60
           MEVRPNPTADNSSI QHHLT+SPLKQPAASSNPIDS AVAQRLQKELMSLMMSGGDLGVS
Sbjct: 1   MEVRPNPTADNSSIPQHHLTTSPLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGVS 60

Query: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYG 120
           AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSL+FPMDYPFKPPLVKFET+CFHPNVDQYG
Sbjct: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLNFPMDYPFKPPLVKFETLCFHPNVDQYG 120

Query: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQYF 180
           NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNN EDYRKMVHKQYF
Sbjct: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNTEDYRKMVHKQYF 180

Query: 181 SGAVLDG 188
           SGAVLDG
Sbjct: 181 SGAVLDG 187

[Close]

BLAST of Spg010035 vs. NCBI nr
Match: XP_038893450.1 (probable ubiquitin-conjugating enzyme E2 C isoform X1 [Benincasa hispida])

HSP 1 Score: 374.0 bits (959), Expect = 7.5e-100
Identity = 179/187 (95.72%), Postives = 183/187 (97.86%), Query Frame = 0


Query: 1   MEVRPNPTADNSSISQHHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVS 60
           MEVRPNPT+DNSS+ QHHL SSPLKQPAASSNPIDS AVAQRLQKELMSLMMSGGDLGVS
Sbjct: 1   MEVRPNPTSDNSSMLQHHLISSPLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGVS 60

Query: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYG 120
           AFP+GESIFTWIGTIEGGKGT YEGLSYKLSLHFPM+YPFKPPLVKFETMCFHPNVDQYG
Sbjct: 61  AFPDGESIFTWIGTIEGGKGTTYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQYG 120

Query: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQYF 180
           NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQYF
Sbjct: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQYF 180

Query: 181 SGAVLDG 188
           SGAVLDG
Sbjct: 181 SGAVLDG 187

[Close]

BLAST of Spg010035 vs. NCBI nr
Match: KAG6573830.1 (Ubiquitin-conjugating enzyme E2 19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012897.1 Ubiquitin-conjugating enzyme E2 19 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 368.2 bits (944), Expect = 4.1e-98
Identity = 178/187 (95.19%), Postives = 181/187 (96.79%), Query Frame = 0


Query: 1   MEVRPNPTADNSSISQHHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVS 60
           MEVRPNPT DNSSISQ HLTSS LKQP+ASSNPIDS AVAQRLQKELMSLMMSG DLGVS
Sbjct: 1   MEVRPNPTGDNSSISQQHLTSSSLKQPSASSNPIDSNAVAQRLQKELMSLMMSGDDLGVS 60

Query: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYG 120
           AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPM+YPFKPPLVKFETMCFHPNVDQYG
Sbjct: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQYG 120

Query: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQYF 180
           NICLDILQDKWSSAYDCRTILLSIQSLLGE NPESPLNSYAAALWNNKEDYRKMVHKQYF
Sbjct: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGESNPESPLNSYAAALWNNKEDYRKMVHKQYF 180

Query: 181 SGAVLDG 188
           SGAV+DG
Sbjct: 181 SGAVMDG 187

[Close]

BLAST of Spg010035 vs. NCBI nr
Match: XP_004142430.1 (probable ubiquitin-conjugating enzyme E2 C isoform X1 [Cucumis sativus] >KGN52282.1 hypothetical protein Csa_008141 [Cucumis sativus])

HSP 1 Score: 366.7 bits (940), Expect = 1.2e-97
Identity = 178/188 (94.68%), Postives = 183/188 (97.34%), Query Frame = 0


Query: 1   MEVRPNPTADNSSISQ-HHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPT +NSS+SQ HHL SS LKQPAASSNPIDS AVAQRLQKELMSLMMSGGDLGV
Sbjct: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60

Query: 61  SAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQY 120
           SAFP+GESIFTWIGTIEGGKGT+YEGLSYKLSLHFPM+YPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180
           GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180

Query: 181 FSGAVLDG 188
           FSGAVLDG
Sbjct: 181 FSGAVLDG 188

[Close]

BLAST of Spg010035 vs. NCBI nr
Match: XP_023542480.1 (probable ubiquitin-conjugating enzyme E2 C isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 366.7 bits (940), Expect = 1.2e-97
Identity = 177/187 (94.65%), Postives = 181/187 (96.79%), Query Frame = 0


Query: 1   MEVRPNPTADNSSISQHHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVS 60
           MEVRPNPT DNSSISQ HLTSS LKQP+ASSNPIDS AVAQRLQKELMSLMMSG DLGVS
Sbjct: 1   MEVRPNPTGDNSSISQQHLTSSSLKQPSASSNPIDSNAVAQRLQKELMSLMMSGDDLGVS 60

Query: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYG 120
           AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPM+YPFKPPLVKFETMCFHPNVDQYG
Sbjct: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQYG 120

Query: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQYF 180
           NICLDILQDKWSSAYDCRTILLSIQSLLGE NPESPLNSYAAALWNNKEDYRKMVHKQYF
Sbjct: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGESNPESPLNSYAAALWNNKEDYRKMVHKQYF 180

Query: 181 SGAVLDG 188
           SGAV++G
Sbjct: 181 SGAVMEG 187

[Close]

BLAST of Spg010035 vs. ExPASy Swiss-Prot
Match: Q8L7T3 (Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 3.3e-58
Identity = 111/159 (69.81%), Postives = 125/159 (78.62%), Query Frame = 0


Query: 21  SSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKG 80
           S+  KQPAA +  +DS +V +RLQ ELM LMM GG  G+SAFPE ++IF W GTI G K 
Sbjct: 18  SNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGP-GISAFPEEDNIFCWKGTITGSKD 77

Query: 81  TAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTI 140
           T +EG  Y+LSL F  DYPFKPP VKFET CFHPNVD YGNICLDILQDKWSSAYD RTI
Sbjct: 78  TVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICLDILQDKWSSAYDVRTI 137

Query: 141 LLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180
           LLSIQSLLGEPN  SPLN+ AA LW+N+E+YRKMV K Y
Sbjct: 138 LLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLY 175

[Close]

BLAST of Spg010035 vs. ExPASy Swiss-Prot
Match: Q9LJZ5 (Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 3.4e-55
Identity = 107/155 (69.03%), Postives = 120/155 (77.42%), Query Frame = 0


Query: 25  KQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTAYE 84
           KQ A  +  +DS +V +RLQ ELM LMM G D G+SAFPE ++IF W GTI G K T +E
Sbjct: 23  KQSAPPTKTVDSHSVLKRLQSELMGLMM-GADPGISAFPEEDNIFCWKGTITGSKDTVFE 82

Query: 85  GLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSI 144
           G  Y+LSL F  DYPFK P VKFET CFHPNVD YGNICLDILQDKWSSAYD RTILLSI
Sbjct: 83  GTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNICLDILQDKWSSAYDVRTILLSI 142

Query: 145 QSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180
           QSLLGEPN  SPLN+ AA LW+N+E+YRKMV K Y
Sbjct: 143 QSLLGEPNISSPLNNQAAQLWSNQEEYRKMVEKLY 176

[Close]

BLAST of Spg010035 vs. ExPASy Swiss-Prot
Match: Q54XS6 (Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum OX=44689 GN=ube2c PE=3 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 5.5e-53
Identity = 93/142 (65.49%), Postives = 120/142 (84.51%), Query Frame = 0


Query: 38  AVAQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMD 97
           +V++RLQ ELM+LMM G   G+SAFP+G++IF WIGTI+G K T YE + +K+SL FP D
Sbjct: 6   SVSKRLQSELMNLMM-GPSPGISAFPDGDNIFNWIGTIQGAKDTVYEQMEFKMSLKFPTD 65

Query: 98  YPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPL 157
           YP+KPP VKFET CFHPNVD  GNICLDIL+DKWS  Y+ R++L+SIQSLLGEPN ESPL
Sbjct: 66  YPYKPPTVKFETQCFHPNVDNNGNICLDILKDKWSPVYNVRSLLISIQSLLGEPNNESPL 125

Query: 158 NSYAAALWNNKEDYRKMVHKQY 180
           NSYAA+LW+N+++Y+K++ K+Y
Sbjct: 126 NSYAASLWSNQDEYKKVLDKRY 146

[Close]

BLAST of Spg010035 vs. ExPASy Swiss-Prot
Match: P56616 (Ubiquitin-conjugating enzyme E2 C OS=Xenopus laevis OX=8355 GN=ube2c PE=1 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.7e-52
Identity = 96/142 (67.61%), Postives = 114/142 (80.28%), Query Frame = 0


Query: 38  AVAQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMD 97
           +V +RLQ+ELM+LMMS GD G+SAFPE +++F WIGTI+G  GT YE L YKLSL FP  
Sbjct: 30  SVGKRLQQELMTLMMS-GDKGISAFPESDNLFKWIGTIDGAVGTVYEDLRYKLSLEFPSG 89

Query: 98  YPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPL 157
           YP+  P VKF T CFHPNVD +GNICLDIL+DKWS+ YD RTILLS+QSLLGEPN ESPL
Sbjct: 90  YPYNAPTVKFVTPCFHPNVDSHGNICLDILKDKWSALYDVRTILLSLQSLLGEPNNESPL 149

Query: 158 NSYAAALWNNKEDYRKMVHKQY 180
           N YAA LW N+  Y+K +H+QY
Sbjct: 150 NPYAAELWQNQTAYKKHLHEQY 170

[Close]

BLAST of Spg010035 vs. ExPASy Swiss-Prot
Match: Q95044 (Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima OX=6584 GN=UBE2C PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.8e-49
Identity = 86/155 (55.48%), Postives = 125/155 (80.65%), Query Frame = 0


Query: 25  KQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTAYE 84
           ++P   +   +  +V++RLQ+EL +L+MS GD G++AFP+G+++F W+ T++G K T YE
Sbjct: 17  ERPRDMTTSKERHSVSKRLQQELRTLLMS-GDPGITAFPDGDNLFKWVATLDGPKDTVYE 76

Query: 85  GLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSI 144
            L YKL+L FP DYP+KPP+VKF T C+HPNVDQ GNICLDIL++ W+++YD RTILLS+
Sbjct: 77  SLKYKLTLEFPSDYPYKPPVVKFTTPCWHPNVDQSGNICLDILKENWTASYDVRTILLSL 136

Query: 145 QSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180
           QSLLGEPN  SPLN+ AA +W+N+ +Y+K++H++Y
Sbjct: 137 QSLLGEPNNASPLNAQAADMWSNQTEYKKVLHEKY 170

[Close]

BLAST of Spg010035 vs. ExPASy TrEMBL
Match: A0A6J1CBM1 (probable ubiquitin-conjugating enzyme E2 C OS=Momordica charantia OX=3673 GN=LOC111010030 PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 9.5e-101
Identity = 181/187 (96.79%), Postives = 184/187 (98.40%), Query Frame = 0


Query: 1   MEVRPNPTADNSSISQHHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVS 60
           MEVRPNPTADNSSI QHHLT+SPLKQPAASSNPIDS AVAQRLQKELMSLMMSGGDLGVS
Sbjct: 1   MEVRPNPTADNSSIPQHHLTTSPLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGVS 60

Query: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYG 120
           AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSL+FPMDYPFKPPLVKFET+CFHPNVDQYG
Sbjct: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLNFPMDYPFKPPLVKFETLCFHPNVDQYG 120

Query: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQYF 180
           NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNN EDYRKMVHKQYF
Sbjct: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNTEDYRKMVHKQYF 180

Query: 181 SGAVLDG 188
           SGAVLDG
Sbjct: 181 SGAVLDG 187

[Close]

BLAST of Spg010035 vs. ExPASy TrEMBL
Match: A0A0A0KV09 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623460 PE=3 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 5.8e-98
Identity = 178/188 (94.68%), Postives = 183/188 (97.34%), Query Frame = 0


Query: 1   MEVRPNPTADNSSISQ-HHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPT +NSS+SQ HHL SS LKQPAASSNPIDS AVAQRLQKELMSLMMSGGDLGV
Sbjct: 1   MEVRPNPTPENSSMSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60

Query: 61  SAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQY 120
           SAFP+GESIFTWIGTIEGGKGT+YEGLSYKLSLHFPM+YPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPDGESIFTWIGTIEGGKGTSYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180
           GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180

Query: 181 FSGAVLDG 188
           FSGAVLDG
Sbjct: 181 FSGAVLDG 188

[Close]

BLAST of Spg010035 vs. ExPASy TrEMBL
Match: A0A5D3CDL5 (Putative ubiquitin-conjugating enzyme E2 C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002790 PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 9.9e-98
Identity = 177/188 (94.15%), Postives = 182/188 (96.81%), Query Frame = 0


Query: 1   MEVRPNPTADNSSISQ-HHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPT +NSS+SQ HHL SS LKQPAASSNPIDS AVAQRLQKELMSLMMSGGDLGV
Sbjct: 1   MEVRPNPTPENSSVSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60

Query: 61  SAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQY 120
           SAFP+GESIFTWIGTI+GGKGT YEGLSYKLSLHFPM+YPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPDGESIFTWIGTIQGGKGTTYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180
           GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180

Query: 181 FSGAVLDG 188
           FSGAVLDG
Sbjct: 181 FSGAVLDG 188

[Close]

BLAST of Spg010035 vs. ExPASy TrEMBL
Match: A0A1S3BH18 (probable ubiquitin-conjugating enzyme E2 C OS=Cucumis melo OX=3656 GN=LOC103489475 PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 9.9e-98
Identity = 177/188 (94.15%), Postives = 182/188 (96.81%), Query Frame = 0


Query: 1   MEVRPNPTADNSSISQ-HHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGV 60
           MEVRPNPT +NSS+SQ HHL SS LKQPAASSNPIDS AVAQRLQKELMSLMMSGGDLGV
Sbjct: 1   MEVRPNPTPENSSVSQHHHLISSSLKQPAASSNPIDSNAVAQRLQKELMSLMMSGGDLGV 60

Query: 61  SAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQY 120
           SAFP+GESIFTWIGTI+GGKGT YEGLSYKLSLHFPM+YPFKPPLVKFETMCFHPNVDQY
Sbjct: 61  SAFPDGESIFTWIGTIQGGKGTTYEGLSYKLSLHFPMEYPFKPPLVKFETMCFHPNVDQY 120

Query: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180
           GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY
Sbjct: 121 GNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180

Query: 181 FSGAVLDG 188
           FSGAVLDG
Sbjct: 181 FSGAVLDG 188

[Close]

BLAST of Spg010035 vs. ExPASy TrEMBL
Match: A0A6J1G1M3 (probable ubiquitin-conjugating enzyme E2 C isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449838 PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 4.9e-97
Identity = 176/186 (94.62%), Postives = 179/186 (96.24%), Query Frame = 0


Query: 1   MEVRPNPTADNSSISQHHLTSSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVS 60
           MEVRPNPT DNSSISQ HLTSS LKQP+ASSNPIDS AVAQRLQKELMSLMMSG DLGVS
Sbjct: 1   MEVRPNPTGDNSSISQQHLTSSSLKQPSASSNPIDSNAVAQRLQKELMSLMMSGDDLGVS 60

Query: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYG 120
           AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPM+YPFKPPLVKFET CFHPNVDQYG
Sbjct: 61  AFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMEYPFKPPLVKFETTCFHPNVDQYG 120

Query: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQYF 180
           NICLDILQDKWSSAYDCRTILLSIQSLLGE NPESPLNSYAAALWNNKEDYRKMVHKQYF
Sbjct: 121 NICLDILQDKWSSAYDCRTILLSIQSLLGESNPESPLNSYAAALWNNKEDYRKMVHKQYF 180

Query: 181 SGAVLD 187
           SGAV+D
Sbjct: 181 SGAVMD 186

[Close]

BLAST of Spg010035 vs. TAIR 10
Match: AT1G50490.1 (ubiquitin-conjugating enzyme 20 )

HSP 1 Score: 226.1 bits (575), Expect = 2.3e-59
Identity = 111/159 (69.81%), Postives = 125/159 (78.62%), Query Frame = 0


Query: 21  SSPLKQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKG 80
           S+  KQPAA +  +DS +V +RLQ ELM LMM GG  G+SAFPE ++IF W GTI G K 
Sbjct: 18  SNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGP-GISAFPEEDNIFCWKGTITGSKD 77

Query: 81  TAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTI 140
           T +EG  Y+LSL F  DYPFKPP VKFET CFHPNVD YGNICLDILQDKWSSAYD RTI
Sbjct: 78  TVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICLDILQDKWSSAYDVRTI 137

Query: 141 LLSIQSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180
           LLSIQSLLGEPN  SPLN+ AA LW+N+E+YRKMV K Y
Sbjct: 138 LLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLY 175

[Close]

BLAST of Spg010035 vs. TAIR 10
Match: AT3G20060.1 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 216.1 bits (549), Expect = 2.4e-56
Identity = 107/155 (69.03%), Postives = 120/155 (77.42%), Query Frame = 0


Query: 25  KQPAASSNPIDSTAVAQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTAYE 84
           KQ A  +  +DS +V +RLQ ELM LMM G D G+SAFPE ++IF W GTI G K T +E
Sbjct: 23  KQSAPPTKTVDSHSVLKRLQSELMGLMM-GADPGISAFPEEDNIFCWKGTITGSKDTVFE 82

Query: 85  GLSYKLSLHFPMDYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSI 144
           G  Y+LSL F  DYPFK P VKFET CFHPNVD YGNICLDILQDKWSSAYD RTILLSI
Sbjct: 83  GTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNICLDILQDKWSSAYDVRTILLSI 142

Query: 145 QSLLGEPNPESPLNSYAAALWNNKEDYRKMVHKQY 180
           QSLLGEPN  SPLN+ AA LW+N+E+YRKMV K Y
Sbjct: 143 QSLLGEPNISSPLNNQAAQLWSNQEEYRKMVEKLY 176

[Close]

BLAST of Spg010035 vs. TAIR 10
Match: AT3G20060.2 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 198.4 bits (503), Expect = 5.2e-51
Identity = 96/132 (72.73%), Postives = 105/132 (79.55%), Query Frame = 0


Query: 48  MSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKF 107
           M LMM G D G+SAFPE ++IF W GTI G K T +EG  Y+LSL F  DYPFK P VKF
Sbjct: 1   MGLMM-GADPGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKF 60

Query: 108 ETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWNN 167
           ET CFHPNVD YGNICLDILQDKWSSAYD RTILLSIQSLLGEPN  SPLN+ AA LW+N
Sbjct: 61  ETCCFHPNVDLYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSN 120

Query: 168 KEDYRKMVHKQY 180
           +E+YRKMV K Y
Sbjct: 121 QEEYRKMVEKLY 131

[Close]

BLAST of Spg010035 vs. TAIR 10
Match: AT5G62540.1 (ubiquitin-conjugating enzyme 3 )

HSP 1 Score: 124.8 bits (312), Expect = 7.4e-29
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 0


Query: 57  LGVSAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFPMDYPFKPPLVKFETMCFHPNV 116
           +G+S  P+  +I  W   I G + T ++G ++KL+LHF  DYP KPP+V+F +  FHPN+
Sbjct: 22  VGISGAPQDNNIMHWNALIFGPEDTPWDGGTFKLTLHFTEDYPNKPPIVRFVSRMFHPNI 81

Query: 117 DQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPESPLNSYAAALWN-NKEDYRKMV 176
              G+ICLDILQ++WS  YD   +L SIQSLL +PNP+SP N+ AA L++ NK +Y + V
Sbjct: 82  YADGSICLDILQNQWSPIYDVAAVLTSIQSLLCDPNPDSPANAEAARLFSENKREYNRKV 141

[Close]

BLAST of Spg010035 vs. TAIR 10
Match: AT1G14400.1 (ubiquitin carrier protein 1 )

HSP 1 Score: 121.7 bits (304), Expect = 6.2e-28
Identity = 63/141 (44.68%), Postives = 86/141 (60.99%), Query Frame = 0


Query: 36  STAVAQRLQKELMSLMMSGGDLGVSAFPEGESIFTWIGTIEGGKGTAYEGLSYKLSLHFP 95
           ST   +RL ++   L       G+S  P+  +I  W   I G   T ++G ++KLSL F 
Sbjct: 2   STPARKRLMRDFKRLQQD-PPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFS 61

Query: 96  MDYPFKPPLVKFETMCFHPNVDQYGNICLDILQDKWSSAYDCRTILLSIQSLLGEPNPES 155
            DYP KPP V+F +  FHPN+   G+ICLDILQ++WS  YD   IL SIQSLL +PNP S
Sbjct: 62  EDYPNKPPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNS 121

Query: 156 PLNSYAAALWN-NKEDYRKMV 176
           P NS AA +++ +K +Y + V
Sbjct: 122 PANSEAARMYSESKREYNRRV 141

The following BLAST results are available for this feature:

BLAST of Spg010035 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022138984.1	2.0e-100	96.79	probable ubiquitin-conjugating enzyme E2 C [Momordica charantia]	[more]
XP_038893450.1	7.5e-100	95.72	probable ubiquitin-conjugating enzyme E2 C isoform X1 [Benincasa hispida]	[more]
KAG6573830.1	4.1e-98	95.19	Ubiquitin-conjugating enzyme E2 19, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_004142430.1	1.2e-97	94.68	probable ubiquitin-conjugating enzyme E2 C isoform X1 [Cucumis sativus] >KGN5228...	[more]
XP_023542480.1	1.2e-97	94.65	probable ubiquitin-conjugating enzyme E2 C isoform X2 [Cucurbita pepo subsp. pep...	[more]

BLAST of Spg010035 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L7T3	3.3e-58	69.81	Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2...	[more]
Q9LJZ5	3.4e-55	69.03	Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1...	[more]
Q54XS6	5.5e-53	65.49	Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum OX=44689 ...	[more]
P56616	2.7e-52	67.61	Ubiquitin-conjugating enzyme E2 C OS=Xenopus laevis OX=8355 GN=ube2c PE=1 SV=1	[more]
Q95044	2.8e-49	55.48	Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima OX=6584 GN=UBE2C PE=1 S...	[more]

BLAST of Spg010035 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CBM1	9.5e-101	96.79	probable ubiquitin-conjugating enzyme E2 C OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A0A0KV09	5.8e-98	94.68	UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G623460 PE...	[more]
A0A5D3CDL5	9.9e-98	94.15	Putative ubiquitin-conjugating enzyme E2 C OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BH18	9.9e-98	94.15	probable ubiquitin-conjugating enzyme E2 C OS=Cucumis melo OX=3656 GN=LOC1034894...	[more]
A0A6J1G1M3	4.9e-97	94.62	probable ubiquitin-conjugating enzyme E2 C isoform X2 OS=Cucurbita moschata OX=3...	[more]

BLAST of Spg010035 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G50490.1	2.3e-59	69.81	ubiquitin-conjugating enzyme 20	[more]
AT3G20060.1	2.4e-56	69.03	ubiquitin-conjugating enzyme19	[more]
AT3G20060.2	5.2e-51	72.73	ubiquitin-conjugating enzyme19	[more]
AT5G62540.1	7.4e-29	48.33	ubiquitin-conjugating enzyme 3	[more]
AT1G14400.1	6.2e-28	44.68	ubiquitin carrier protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00212	ubc_7	coord: 41..184 e-value: 4.9E-54 score: 195.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..34
None	No IPR available	PANTHER	PTHR24068	UBIQUITIN-CONJUGATING ENZYME E2	coord: 21..182
None	No IPR available	PANTHER	PTHR24068:SF320	UBIQUITIN-CONJUGATING ENZYME E2 C-RELATED	coord: 21..182
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 33..181 e-value: 1.4E-53 score: 182.6
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 35..183
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 42..177 e-value: 2.5E-43 score: 147.1
IPR000608	Ubiquitin-conjugating enzyme E2	PROSITE	PS50127	UBC_2	coord: 38..184 score: 44.460594
IPR000608	Ubiquitin-conjugating enzyme E2	CDD	cd00195	UBCc	coord: 41..175 e-value: 9.81991E-61 score: 183.558
IPR023313	Ubiquitin-conjugating enzyme, active site	PROSITE	PS00183	UBC_1	coord: 112..127

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg010035.1	Spg010035.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031145	anaphase-promoting complex-dependent catabolic process
biological_process	GO:0006952	defense response
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0030071	regulation of mitotic metaphase/anaphase transition
biological_process	GO:0009607	response to biotic stimulus
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0061631	ubiquitin conjugating enzyme activity