Spg008651 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg008651
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Eukaryotic aspartyl protease family protein
Location	scaffold10: 34846901 .. 34848055 (+)
RNA-Seq Expression	Spg008651
Synteny	Spg008651

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCCGACGAGGAATCGAGCGCGACAAGACCCGGATACAGAATTTCAATATGATGGCCATAAGTAACTTCGATGTTGAAATGCCTGTTGTTGAGAGAAAGAGAAGCTATCTGATGAACTTGTCGCTCAGGATGCCTCCAGTTTCGTTCTCTGCCATTGCCGATACTGGCAGCGACTTGATTTGGACGCAGTGCAAGCCATGCTTGCAATGCTTTGAGCAGCCAAGCCCTGTGTATGATCCTGCACGATCGTCTTCCTTTGCCAATGCCACTTCCTCTGCTGCATTGTGCAAGGCTTTGACCAATTCTAGTTCCAGCAATGGTTGTGAGTATTCGGTTGGTTATGGAGACGGGTCTTCGACCAAAGGTTATTTAGCATTCGAGACTTTGACGCTCGGAGAAGCAAACCAGCAAGTTTCTGCACCAGACATAGCTTTCGGGTGCGGGCTAAAGAACCACGTGAAGGGCCTGAAACAGGGTGCTGGCATGGTGGGACTCAGTCGAGGACCATTGTCGCTTATTTCGCAGCTCGACATTCACAAATTTTCTTACTGTTTAACTCCAAATGGAACTGGGAGTCTGAACTTAGAATCTTCAACAAGTATCGACAACACTACGAATCGAGCCATCAAGGTCACACCGCTGATACAAAATCCATTTGACCCATCTTTCTACTATCTATCTCTACGGGGAATCACAGTAGGCCAAAAGTTCTTGCCCATCCCGTCCTCGTGGTTTAGACTAAACACCGATGGCAGCGGCGACGTTATTATTGATTCTGGCACATCGATCACCTACTTAACATAGGATGCTTTTGATGTGCTGAAACCATTTTTCACTGCCCAGACAAACCTCCCAGTGAAACATTCAGCAGGCATTGGTCTTGATCTCTGCTTTGAGCTGCCTTCCCACAACAAAAGCAAACTTGATGTGCCTGATTTGGTATTTCATTTCGAGGGGCTCAACTTGAAGCTTCCAGTCGAGAACTATATGGTCACCGATGAGGAGCGGGGGTTGGTGTGCTTGGCAATGGGAGCTGCAGGAGCTTTGTCAATTTTCGGCAGCATGCAACACCAGAATATGTTGGTTCTTCATGATCTCAAGAAGGAAGTTGTATCATTTGTTCCCACACAGTGTGCTCGGCTCGGTAACTAG

mRNA sequence

ATGCTCCGACGAGGAATCGAGCGCGACAAGACCCGGATACAGAATTTCAATATGATGGCCATAAGTAACTTCGATGTTGAAATGCCTGTTGTTGAGAGAAAGAGAAGCTATCTGATGAACTTGTCGCTCAGGATGCCTCCAGTTTCGTTCTCTGCCATTGCCGATACTGGCAGCGACTTGATTTGGACGCAGTGCAAGCCATGCTTGCAATGCTTTGAGCAGCCAAGCCCTGTGTATGATCCTGCACGATCGTCTTCCTTTGCCAATGCCACTTCCTCTGCTGCATTGTGCAAGGCTTTGACCAATTCTAGTTCCAGCAATGGTTGTGAGTATTCGGTTGGTTATGGAGACGGGTCTTCGACCAAAGGTTATTTAGCATTCGAGACTTTGACGCTCGGAGAAGCAAACCAGCAAGTTTCTGCACCAGACATAGCTTTCGGGTGCGGGCTAAAGAACCACGTGAAGGGCCTGAAACAGGGTGCTGGCATGGTGGGACTCAGTCGAGGACCATTGTCGCTTATTTCGCAGCTCGACATTCACAAATTTTCTTACTGTTTAACTCCAAATGGAACTGGGAGTCTGAACTTAGAATCTTCAACAAGTATCGACAACACTACGAATCGAGCCATCAAGGATGCTTTTGATGTGCTGAAACCATTTTTCACTGCCCAGACAAACCTCCCAGTGAAACATTCAGCAGGCATTGGTCTTGATCTCTGCTTTGAGCTGCCTTCCCACAACAAAAGCAAACTTGATGTGCCTGATTTGGTATTTCATTTCGAGGGGCTCAACTTGAAGCTTCCAGTCGAGAACTATATGGTCACCGATGAGGAGCGGGGGTTGGTGTGCTTGGCAATGGGAGCTGCAGGAGCTTTGTCAATTTTCGGCAGCATGCAACACCAGAATATGTTGGTTCTTCATGATCTCAAGAAGGAAGTTGTATCATTTGTTCCCACACAGTGTGCTCGGCTCGGTAACTAG

Coding sequence (CDS)

ATGCTCCGACGAGGAATCGAGCGCGACAAGACCCGGATACAGAATTTCAATATGATGGCCATAAGTAACTTCGATGTTGAAATGCCTGTTGTTGAGAGAAAGAGAAGCTATCTGATGAACTTGTCGCTCAGGATGCCTCCAGTTTCGTTCTCTGCCATTGCCGATACTGGCAGCGACTTGATTTGGACGCAGTGCAAGCCATGCTTGCAATGCTTTGAGCAGCCAAGCCCTGTGTATGATCCTGCACGATCGTCTTCCTTTGCCAATGCCACTTCCTCTGCTGCATTGTGCAAGGCTTTGACCAATTCTAGTTCCAGCAATGGTTGTGAGTATTCGGTTGGTTATGGAGACGGGTCTTCGACCAAAGGTTATTTAGCATTCGAGACTTTGACGCTCGGAGAAGCAAACCAGCAAGTTTCTGCACCAGACATAGCTTTCGGGTGCGGGCTAAAGAACCACGTGAAGGGCCTGAAACAGGGTGCTGGCATGGTGGGACTCAGTCGAGGACCATTGTCGCTTATTTCGCAGCTCGACATTCACAAATTTTCTTACTGTTTAACTCCAAATGGAACTGGGAGTCTGAACTTAGAATCTTCAACAAGTATCGACAACACTACGAATCGAGCCATCAAGGATGCTTTTGATGTGCTGAAACCATTTTTCACTGCCCAGACAAACCTCCCAGTGAAACATTCAGCAGGCATTGGTCTTGATCTCTGCTTTGAGCTGCCTTCCCACAACAAAAGCAAACTTGATGTGCCTGATTTGGTATTTCATTTCGAGGGGCTCAACTTGAAGCTTCCAGTCGAGAACTATATGGTCACCGATGAGGAGCGGGGGTTGGTGTGCTTGGCAATGGGAGCTGCAGGAGCTTTGTCAATTTTCGGCAGCATGCAACACCAGAATATGTTGGTTCTTCATGATCTCAAGAAGGAAGTTGTATCATTTGTTCCCACACAGTGTGCTCGGCTCGGTAACTAG

Protein sequence

MLRRGIERDKTRIQNFNMMAISNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGSDLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDGSSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLDIHKFSYCLTPNGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAGIGLDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSIFGSMQHQNMLVLHDLKKEVVSFVPTQCARLGN

Homology

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BLAST of Spg008651 vs. NCBI nr
Match: XP_038886455.1 (aspartic proteinase nepenthesin-2-like [Benincasa hispida])

HSP 1 Score: 489.2 bits (1258), Expect = 2.8e-134
Identity = 257/382 (67.28%), Postives = 284/382 (74.35%), Query Frame = 0


Query: 1   MLRRGIERDKTRIQNFNMMAISNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGSDL 60
           +LRRGI+RDKT IQNFN M ISN  V MPVVE++ SYLMNLSL  PP+SFSAI DTGSDL
Sbjct: 50  ILRRGIKRDKTWIQNFNKMIISNLKVRMPVVEQRGSYLMNLSLGTPPISFSAIFDTGSDL 109

Query: 61  IWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDGSS 120
           +WTQCKPCLQCF+QP+PVYDPA+SSSFANAT SAALCK LTNSSSSNGCEYS  Y D S 
Sbjct: 110 VWTQCKPCLQCFKQPTPVYDPAQSSSFANATRSAALCKELTNSSSSNGCEYSFSYADESF 169

Query: 121 TKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLDIH 180
           T GYLAFETLTLG ANQ+VS PDIAFGCG+KNHVKGL QGAG+VG +RGPLSL+SQL I 
Sbjct: 170 TTGYLAFETLTLGGANQKVSTPDIAFGCGIKNHVKGLTQGAGIVGFNRGPLSLLSQLHIK 229

Query: 181 KFSYCLTPNGTGSLNLESSTSIDNTTNRAIK----------------------------- 240
           KFSYCL+ NGTGSL   SSTSIDNTTNRAIK                             
Sbjct: 230 KFSYCLSLNGTGSLVFGSSTSIDNTTNRAIKTTPLIQNPYNPSYYYLSLQGITVGQKYLP 289

Query: 241 -----------------------------DAFDVLKPFFTAQTNLPVKHSAGIGLDLCFE 300
                                        D FDVLKP F +QTNL V  S+ IGLDLCF+
Sbjct: 290 VPSSSFRLNTDGSGGMIIDSGTSMTYITEDVFDVLKPVFASQTNLQVIWSS-IGLDLCFQ 349

Query: 301 LPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSIFGSMQHQN 325
           LPSHN SKLDVPDL+FHFE L+LKLPVENYMV +EE G+VCLAMGAAG LSIFGSMQHQN
Sbjct: 350 LPSHNNSKLDVPDLIFHFEDLDLKLPVENYMVVNEELGIVCLAMGAAGGLSIFGSMQHQN 409

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BLAST of Spg008651 vs. NCBI nr
Match: KAG6737285.1 (hypothetical protein POTOM_058799 [Populus tomentosa])

HSP 1 Score: 322.4 bits (825), Expect = 4.5e-84
Identity = 173/390 (44.36%), Postives = 234/390 (60.00%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAI---SNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGS 61
           ++ G++R + R+Q F  MA+   SN +++ PV+     +LM L++  PP ++SAI DTGS
Sbjct: 59  IQHGVKRGRHRLQRFKAMALVASSNSEIDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGS 118

Query: 62  DLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDG 121
           DLIWTQCKPC QCF+QP+P++DP +SSSF+  + S+ LC+AL  S+ S+GCEY  GYGD 
Sbjct: 119 DLIWTQCKPCTQCFDQPTPIFDPKKSSSFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDY 178

Query: 122 SSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLD 181
           SST+G LA ETLT G    +VS P +AFGCG  N   G  QG+G+VGL RGPLSL+SQL+
Sbjct: 179 SSTQGILASETLTFG----KVSVPKVAFGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLE 238

Query: 182 IHKFSYCLT--------------------------------------------------- 241
             KFSYCLT                                                   
Sbjct: 239 EPKFSYCLTSVDDTKASTLLMGSLASVKSSDSEIKTTPLIQNSAQPSFYYLSLEGISVGD 298

Query: 242 -------------PNGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAG 301
                         +G+G L ++S T+I        + AFD++   FT+Q NLPV +S  
Sbjct: 299 TSLPIKKSTFSLQEDGSGGLIIDSGTTITYLE----QSAFDLVAKEFTSQINLPVDNSGS 358

Query: 302 IGLDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSI 325
            GL++CF LPS   + ++VP LVFHF+G +L+LP ENYM+ D   G+ CLAMG++  +SI
Sbjct: 359 TGLEVCFTLPS-GSTDIEVPKLVFHFDGADLELPAENYMIADASMGVACLAMGSSSGMSI 418

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BLAST of Spg008651 vs. NCBI nr
Match: XP_011046330.1 (PREDICTED: aspartic proteinase nepenthesin-1-like [Populus euphratica])

HSP 1 Score: 321.6 bits (823), Expect = 7.7e-84
Identity = 172/390 (44.10%), Postives = 233/390 (59.74%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAI---SNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGS 61
           ++ G++R + R+Q F  MA+   SN +++ PV+     +LMNL++  PP ++SAI DTGS
Sbjct: 59  IQHGVKRGRHRLQRFKAMALVASSNSEIDAPVLPGNGEFLMNLAIGTPPATYSAIMDTGS 118

Query: 62  DLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDG 121
           DLIWTQCKPC QCF+QP+P++DP +SSSF+  + S+ LC+AL  S+ S+GCEY  GYGD 
Sbjct: 119 DLIWTQCKPCTQCFDQPTPIFDPKKSSSFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDY 178

Query: 122 SSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLD 181
           SST+G LA ETLT G    +VS P +AFGCG  N   G  QG+G+VGL RGPLSL+SQL 
Sbjct: 179 SSTQGILASETLTFG----KVSVPKVAFGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLK 238

Query: 182 IHKFSYCLT--------------------------------------------------- 241
             KFSYCLT                                                   
Sbjct: 239 EPKFSYCLTSVDDTKASTLLMGSLASVKASDSEIKSTPLIQNSAQPSFYYLSLEGISVGD 298

Query: 242 -------------PNGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAG 301
                         +G+G L ++S T+I        + AFD++   FT+Q NLPV +S  
Sbjct: 299 TSLPIKKSTFSLQEDGSGGLIIDSGTTITYLE----QSAFDLVSKEFTSQMNLPVDNSGA 358

Query: 302 IGLDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSI 325
            GL++CF LPS   + ++VP LVFHF+G +L+LP ENYM+ D   G+ CLAMG++  +SI
Sbjct: 359 TGLEVCFTLPS-GSTDIEVPKLVFHFDGADLELPAENYMIADASMGVACLAMGSSSGMSI 418

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BLAST of Spg008651 vs. NCBI nr
Match: XP_006370905.1 (aspartic proteinase nepenthesin-1 [Populus trichocarpa] >PNS89732.1 hypothetical protein POPTR_019G002100 [Populus trichocarpa])

HSP 1 Score: 321.2 bits (822), Expect = 1.0e-83
Identity = 172/390 (44.10%), Postives = 234/390 (60.00%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAI---SNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGS 61
           ++ G++R + R+Q F  MA+   SN +++ PV+     +LM L++  PP ++SAI DTGS
Sbjct: 59  IQHGVKRGRHRLQRFKAMALVASSNSEIDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGS 118

Query: 62  DLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDG 121
           DLIWTQCKPC QCF+QP+P++DP +SSSF+  + S+ LC+AL  S+ S+GCEY  GYGD 
Sbjct: 119 DLIWTQCKPCTQCFDQPTPIFDPKKSSSFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDY 178

Query: 122 SSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLD 181
           SST+G LA ETLT G    +VS P++AFGCG  N   G  QG+G+VGL RGPLSL+SQL 
Sbjct: 179 SSTQGMLASETLTFG----KVSVPEVAFGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLK 238

Query: 182 IHKFSYCLT--------------------------------------------------- 241
             KFSYCLT                                                   
Sbjct: 239 EPKFSYCLTSVDDTKASTLLMGSLASVKASDSEIKTTPLIQNSAQPSFYYLSLEGISVGD 298

Query: 242 -------------PNGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAG 301
                         +G+G L ++S T+I        + AFD++   FT+Q NLPV +S  
Sbjct: 299 TSLPIKKSTFSLQEDGSGGLIIDSGTTITYLE----QSAFDLVAKEFTSQINLPVDNSGS 358

Query: 302 IGLDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSI 325
            GL++CF LPS   + ++VP LVFHF+G +L+LP ENYM+ D   G+ CLAMG++  +SI
Sbjct: 359 TGLEVCFTLPS-GSTDIEVPKLVFHFDGADLELPAENYMIADASMGVACLAMGSSSGMSI 418

[Close]

BLAST of Spg008651 vs. NCBI nr
Match: XP_034919474.1 (aspartic proteinase nepenthesin-1-like [Populus alba] >TKS10217.1 aspartyl protease family protein [Populus alba])

HSP 1 Score: 321.2 bits (822), Expect = 1.0e-83
Identity = 172/390 (44.10%), Postives = 234/390 (60.00%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAI---SNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGS 61
           ++ G++R + R+Q F  MA+   SN +++ PV+     +LM L++  PP ++SAI DTGS
Sbjct: 59  IQHGVKRGRHRLQRFKAMALVASSNSEIDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGS 118

Query: 62  DLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDG 121
           DLIWTQCKPC QCF+QP+P++DP +SSSF+  + S+ LC+AL  S+ S+GCEY  GYGD 
Sbjct: 119 DLIWTQCKPCTQCFDQPTPIFDPKKSSSFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDY 178

Query: 122 SSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLD 181
           SST+G LA ETLT G    +VS P +AFGCG  N   G  QG+G+VGL RGPLSL+SQL+
Sbjct: 179 SSTQGILASETLTFG----KVSVPKVAFGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLE 238

Query: 182 IHKFSYCLT--------------------------------------------------- 241
             KFSYCLT                                                   
Sbjct: 239 EPKFSYCLTSVDDTKASTLLMGSLASVKASDSEIKTTPLIQNSAQPSFYYLSLEGISVGD 298

Query: 242 -------------PNGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAG 301
                         +G+G L ++S T+I        + AFD++   FT+Q NLPV +S  
Sbjct: 299 TSLPIKKSTFSLQEDGSGGLIIDSGTTITYLE----QSAFDLVAKEFTSQINLPVDNSGS 358

Query: 302 IGLDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSI 325
            GL++CF LPS   + ++VP LVFHF+G +L+LP ENYM+ D   G+ CLAMG++  +SI
Sbjct: 359 TGLEVCFTLPS-GSTDIEVPKLVFHFDGADLELPAENYMIADASMGVACLAMGSSSGMSI 418

[Close]

BLAST of Spg008651 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.9e-70
Identity = 157/386 (40.67%), Postives = 208/386 (53.89%), Query Frame = 0


Query: 1   MLRRGIERDKTRIQNFNMMAISNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGSDL 60
           +L R IER   R+Q    M      VE  V      YLMNLS+  P   FSAI DTGSDL
Sbjct: 59  LLERAIERGSRRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDL 118

Query: 61  IWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNG-CEYSVGYGDGS 120
           IWTQC+PC QCF Q +P+++P  SSSF+    S+ LC+AL++ + SN  C+Y+ GYGDGS
Sbjct: 119 IWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNFCQYTYGYGDGS 178

Query: 121 STKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLDI 180
            T+G +  ETLT G     VS P+I FGCG  N   G   GAG+VG+ RGPLSL SQLD+
Sbjct: 179 ETQGSMGTETLTFG----SVSIPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDV 238

Query: 181 HKFSYCLTP--------------------------------------------------- 240
            KFSYC+TP                                                   
Sbjct: 239 TKFSYCMTPIGSSTPSNLLLGSLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRL 298

Query: 241 ------------NGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAGIG 300
                       NGTG + ++S T++    N    +A+  ++  F +Q NLPV + +  G
Sbjct: 299 PIDPSAFALNSNNGTGGIIIDSGTTLTYFVN----NAYQSVRQEFISQINLPVVNGSSSG 358

Query: 301 LDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAG-ALSIF 322
            DLCF+ PS + S L +P  V HF+G +L+LP ENY ++    GL+CLAMG++   +SIF
Sbjct: 359 FDLCFQTPS-DPSNLQIPTFVMHFDGGDLELPSENYFIS-PSNGLICLAMGSSSQGMSIF 418

[Close]

BLAST of Spg008651 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 5.9e-63
Identity = 146/382 (38.22%), Postives = 210/382 (54.97%), Query Frame = 0


Query: 1   MLRRGIERDKTRIQNFNMMAISNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGSDL 60
           +++R I+R + R+++ N M  S+  +E PV      YLMN+++  P  SFSAI DTGSDL
Sbjct: 60  LIKRAIKRGERRMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDL 119

Query: 61  IWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKAL-TNSSSSNGCEYSVGYGDGS 120
           IWTQC+PC QCF QP+P+++P  SSSF+     +  C+ L + + ++N C+Y+ GYGDGS
Sbjct: 120 IWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDGS 179

Query: 121 STKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLDI 180
           +T+GY+A ET T     +  S P+IAFGCG  N   G   GAG++G+  GPLSL SQL +
Sbjct: 180 TTQGYMATETFTF----ETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV 239

Query: 181 HKFSYCLTPNGTGS---------------------------------------------L 240
            +FSYC+T  G+ S                                             L
Sbjct: 240 GQFSYCMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGDNL 299

Query: 241 NLESST-----------SIDNTTNRAI--KDAFDVLKPFFTAQTNLPVKHSAGIGLDLCF 300
            + SST            ID+ T      +DA++ +   FT Q NLP    +  GL  CF
Sbjct: 300 GIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCF 359

Query: 301 ELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGAL--SIFGSMQ 322
           + PS + S + VP++   F+G  L L  +N +++  E G++CLAMG++  L  SIFG++Q
Sbjct: 360 QQPS-DGSTVQVPEISMQFDGGVLNLGEQNILISPAE-GVICLAMGSSSQLGISIFGNIQ 419

[Close]

BLAST of Spg008651 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.6e-44
Identity = 124/388 (31.96%), Postives = 181/388 (46.65%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAISNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGSDLI 61
           LR  I R   R+ +F     +    ++ +      YLMN+S+  PP    AIADTGSDL+
Sbjct: 56  LRNAIHRSVNRVFHFTEKD-NTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLL 115

Query: 62  WTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTN----SSSSNGCEYSVGYGD 121
           WTQC PC  C+ Q  P++DP  SS++ + + S++ C AL N    S++ N C YS+ YGD
Sbjct: 116 WTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGD 175

Query: 122 GSSTKGYLAFETLTLGEAN-QQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQ 181
            S TKG +A +TLTLG ++ + +   +I  GCG  N     K+G+G+VGL  GP+SLI Q
Sbjct: 176 NSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQ 235

Query: 182 LDIH---KFSYCLTP--------------------------------------------- 241
           L      KFSYCL P                                             
Sbjct: 236 LGDSIDGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKS 295

Query: 242 ----------NGTGSLNLESSTSIDNTTNRAI--KDAFDVLKPFFTAQTNLPVKHSAGIG 301
                     +G+ S + E +  ID+ T   +   + +  L+    +  +   K     G
Sbjct: 296 ISVGSKQIQYSGSDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSG 355

Query: 302 LDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSIFG 325
           L LC+         L VP +  HF+G ++KL   N  V   E  LVC A   + + SI+G
Sbjct: 356 LSLCYSA----TGDLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYG 415

[Close]

BLAST of Spg008651 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 161.8 bits (408), Expect = 1.3e-38
Identity = 124/421 (29.45%), Postives = 181/421 (42.99%), Query Frame = 0


Query: 1   MLRRGIERDKTRIQNFNM------MAISNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIA 60
           +LRR I+R + R+    M       A      E P++     YL+ L +  PP  F+A  
Sbjct: 47  LLRRAIQRSRYRLAGIGMARGEAASARKAVVAETPIMPAGGEYLVKLGIGTPPYKFTAAI 106

Query: 61  DTGSDLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKAL----TNSSSSNGCE 120
           DT SDLIWTQC+PC  C+ Q  P+++P  SS++A    S+  C  L            C+
Sbjct: 107 DTASDLIWTQCQPCTGCYHQVDPMFNPRVSSTYAALPCSSDTCDELDVHRCGHDDDESCQ 166

Query: 121 YSVGYGDGSSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKG-LKQGAGMVGLSRG 180
           Y+  Y   ++T+G LA + L +GE     +   +AFGC   +       Q +G+VGL RG
Sbjct: 167 YTYTYSGNATTEGTLAVDKLVIGED----AFRGVAFGCSTSSTGGAPPPQASGVVGLGRG 226

Query: 181 PLSLISQLDIHKFSYCLTP-------------------NGTGS----------------L 240
           PLSL+SQL + +F+YCL P                   N T                  L
Sbjct: 227 PLSLVSQLSVRRFAYCLPPPASRIPGKLVLGADADAARNATNRIAVPMRRDPRYPSYYYL 286

Query: 241 NLE----------------------------SSTSIDNTTNRAIKDA------------- 300
           NL+                            + T   N T  A+ DA             
Sbjct: 287 NLDGLLIGDRAMSLPPTTTTTATATATAPAPAPTPSPNATAVAVGDANRYGMIIDIASTI 346

Query: 301 -------FDVLKPFFTAQTNLPVKHSAGIGLDLCFELPSHNK-SKLDVPDLVFHFEGLNL 325
                  +D L      +  LP    + +GLDLCF LP      ++ VP +   F+G  L
Sbjct: 347 TFLEASLYDELVNDLEVEIRLPRGTGSSLGLDLCFILPDGVAFDRVYVPAVALAFDGRWL 406

[Close]

BLAST of Spg008651 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-34
Identity = 111/362 (30.66%), Postives = 164/362 (45.30%), Query Frame = 0


Query: 25  DVEMPVV----ERKRSYLMNLSLRMPPVSFSAIADTGSDLIWTQCKPCLQCFEQPSPVYD 84
           D+  PVV    +    Y   + +  P      + DTGSD+ W QC+PC  C++Q  PV++
Sbjct: 146 DLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFN 205

Query: 85  PARSSSFANATSSAALCKAL-TNSSSSNGCEYSVGYGDGSSTKGYLAFETLTLGEANQQV 144
           P  SS++ + T SA  C  L T++  SN C Y V YGDGS T G LA +T+T G + +  
Sbjct: 206 PTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGK-- 265

Query: 145 SAPDIAFGCGLKNHVKGLKQG-AGMVGLSRGPLSLISQLDIHKFSYCLTPNGTG---SLN 204
              ++A GCG  N  +GL  G AG++GL  G LS+ +Q+    FSYCL    +G   SL+
Sbjct: 266 -INNVALGCGHDN--EGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLD 325

Query: 205 LES--------------STSID-------------------------------------- 264
             S              +  ID                                      
Sbjct: 326 FNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDC 385

Query: 265 -NTTNRAIKDAFDVLKPFFTAQTNLPVKHSAGIGL-DLCFELPSHNKSKLDVPDLVFHFE 322
                R    A++ L+  F   T    K S+ I L D C++  S   S + VP + FHF 
Sbjct: 386 GTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSS--LSTVKVPTVAFHFT 445

[Close]

BLAST of Spg008651 vs. ExPASy TrEMBL
Match: A0A2N9HF56 (Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS40859 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 8.0e-87
Identity = 183/386 (47.41%), Postives = 230/386 (59.59%), Query Frame = 0


Query: 6   IERDKTRIQNFNMMA--ISNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGSDLIWT 65
           I R KTR+   + +     N D++ P+      +LM+LS+  P V F AI DTGSDLIWT
Sbjct: 64  INRVKTRLDRLSAITKNAPNDDIQAPIYVGDGEFLMHLSIGTPAVPFLAIMDTGSDLIWT 123

Query: 66  QCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDGSSTKG 125
           QCKPC  CF Q +PV++PA SSSF+N T S+ LCKAL+NS   NGCEY   YGD SS++G
Sbjct: 124 QCKPCSNCFNQSTPVFEPALSSSFSNVTCSSELCKALSNSRCGNGCEYLNRYGDESSSEG 183

Query: 126 YLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLDIHKFS 185
           +LA ETLT GE+ Q+VS P+I FGCG+ N  KG  + AG+VGL RGPLSL+SQL   KFS
Sbjct: 184 FLAMETLTFGESAQRVSVPNIGFGCGVDNKGKGWNKAAGLVGLGRGPLSLVSQLGSKKFS 243

Query: 186 YCLT-------------------------------------------------------- 245
           YCLT                                                        
Sbjct: 244 YCLTSIGDNKSSSLLFGSLADLNLSNGAVLKTTPLIRNPILPSFYYLSLEGITVGQTLLP 303

Query: 246 ---------PNGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAGIGLD 305
                     +G G + L+S T+I +      +DAFDVLK  F AQT L V +    GLD
Sbjct: 304 IPKSLFQLGSDGGGGVILDSGTTITSLH----EDAFDVLKQVFKAQTKLRVSNLYTRGLD 363

Query: 306 LCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSIFGSM 325
           +CF+LPS +   + VP LVFHFEGL+L+LPVENYM+ D + G+VCLAMGA G LSIFG++
Sbjct: 364 VCFDLPSQDVKDIKVPKLVFHFEGLDLELPVENYMIADTKLGVVCLAMGATGRLSIFGNI 423

[Close]

BLAST of Spg008651 vs. ExPASy TrEMBL
Match: A0A6M2F5X8 (Peptidase A1 domain-containing protein OS=Populus davidiana OX=266767 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 9.8e-85
Identity = 174/390 (44.62%), Postives = 233/390 (59.74%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAI---SNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGS 61
           ++ G++R + R+Q F  MA+   SN +++ PV+     +LM L++  PP ++SAI DTGS
Sbjct: 56  IQHGVKRGRHRLQRFKAMALVASSNSEIDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGS 115

Query: 62  DLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDG 121
           DLIWTQCKPC QCF+QP+P++DP +SSSF+  + S+ LC+AL  S+ S+GCEY  GYGD 
Sbjct: 116 DLIWTQCKPCTQCFDQPTPIFDPKKSSSFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDY 175

Query: 122 SSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLD 181
           SST+G LA ETLT G    +VS P +AFGCG  N   G  QG+G+VGL RGPLSL+SQLD
Sbjct: 176 SSTQGILASETLTFG----KVSVPKVAFGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLD 235

Query: 182 IHKFSYCLT--------------------------------------------------- 241
             KFSYCLT                                                   
Sbjct: 236 EPKFSYCLTSVDDTKASTLLMGSLASVKASDSEIKTTPLIQNSAQPSFYYLSLEGISVGA 295

Query: 242 -------------PNGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAG 301
                         +G+G L ++S T+I        + AFD++   FT+Q NLPV  S  
Sbjct: 296 TRLPIKKSTFSLQDDGSGGLIIDSGTTITYLE----QSAFDLVAKEFTSQINLPVDSSGS 355

Query: 302 IGLDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSI 325
            GLD+CF LPS   + ++VP LVFHF+G +L+LP ENYM+ D   G+ CLAMG++  +SI
Sbjct: 356 TGLDVCFTLPS-GSTNIEVPKLVFHFDGADLELPAENYMIGDSSMGVACLAMGSSSGMSI 415

[Close]

BLAST of Spg008651 vs. ExPASy TrEMBL
Match: B9N2J4 (Peptidase A1 domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_019G002100 PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 4.9e-84
Identity = 172/390 (44.10%), Postives = 234/390 (60.00%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAI---SNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGS 61
           ++ G++R + R+Q F  MA+   SN +++ PV+     +LM L++  PP ++SAI DTGS
Sbjct: 59  IQHGVKRGRHRLQRFKAMALVASSNSEIDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGS 118

Query: 62  DLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDG 121
           DLIWTQCKPC QCF+QP+P++DP +SSSF+  + S+ LC+AL  S+ S+GCEY  GYGD 
Sbjct: 119 DLIWTQCKPCTQCFDQPTPIFDPKKSSSFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDY 178

Query: 122 SSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLD 181
           SST+G LA ETLT G    +VS P++AFGCG  N   G  QG+G+VGL RGPLSL+SQL 
Sbjct: 179 SSTQGMLASETLTFG----KVSVPEVAFGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLK 238

Query: 182 IHKFSYCLT--------------------------------------------------- 241
             KFSYCLT                                                   
Sbjct: 239 EPKFSYCLTSVDDTKASTLLMGSLASVKASDSEIKTTPLIQNSAQPSFYYLSLEGISVGD 298

Query: 242 -------------PNGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAG 301
                         +G+G L ++S T+I        + AFD++   FT+Q NLPV +S  
Sbjct: 299 TSLPIKKSTFSLQEDGSGGLIIDSGTTITYLE----QSAFDLVAKEFTSQINLPVDNSGS 358

Query: 302 IGLDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSI 325
            GL++CF LPS   + ++VP LVFHF+G +L+LP ENYM+ D   G+ CLAMG++  +SI
Sbjct: 359 TGLEVCFTLPS-GSTDIEVPKLVFHFDGADLELPAENYMIADASMGVACLAMGSSSGMSI 418

[Close]

BLAST of Spg008651 vs. ExPASy TrEMBL
Match: A0A4U5QLX0 (Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000085760 PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 4.9e-84
Identity = 172/390 (44.10%), Postives = 234/390 (60.00%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAI---SNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGS 61
           ++ G++R + R+Q F  MA+   SN +++ PV+     +LM L++  PP ++SAI DTGS
Sbjct: 59  IQHGVKRGRHRLQRFKAMALVASSNSEIDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGS 118

Query: 62  DLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDG 121
           DLIWTQCKPC QCF+QP+P++DP +SSSF+  + S+ LC+AL  S+ S+GCEY  GYGD 
Sbjct: 119 DLIWTQCKPCTQCFDQPTPIFDPKKSSSFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDY 178

Query: 122 SSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLD 181
           SST+G LA ETLT G    +VS P +AFGCG  N   G  QG+G+VGL RGPLSL+SQL+
Sbjct: 179 SSTQGILASETLTFG----KVSVPKVAFGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLE 238

Query: 182 IHKFSYCLT--------------------------------------------------- 241
             KFSYCLT                                                   
Sbjct: 239 EPKFSYCLTSVDDTKASTLLMGSLASVKASDSEIKTTPLIQNSAQPSFYYLSLEGISVGD 298

Query: 242 -------------PNGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAG 301
                         +G+G L ++S T+I        + AFD++   FT+Q NLPV +S  
Sbjct: 299 TSLPIKKSTFSLQEDGSGGLIIDSGTTITYLE----QSAFDLVAKEFTSQINLPVDNSGS 358

Query: 302 IGLDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSI 325
            GL++CF LPS   + ++VP LVFHF+G +L+LP ENYM+ D   G+ CLAMG++  +SI
Sbjct: 359 TGLEVCFTLPS-GSTDIEVPKLVFHFDGADLELPAENYMIADASMGVACLAMGSSSGMSI 418

[Close]

BLAST of Spg008651 vs. ExPASy TrEMBL
Match: A0A4U5R6H0 (Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000004600 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 8.3e-84
Identity = 172/390 (44.10%), Postives = 234/390 (60.00%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAI---SNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGS 61
           ++ G++R + R+Q F  MA+   SN +++ PV+     +LM L++  PP ++SAI DTGS
Sbjct: 59  IQHGVKRGRHRLQRFKAMALVASSNSEIDAPVLPGNGEFLMKLAIGTPPETYSAIMDTGS 118

Query: 62  DLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSSNGCEYSVGYGDG 121
           DLIWTQCKPC QCF+QP+P++DP +SSSF+  + S+ LC+AL  S+ S+GCEY  GYGD 
Sbjct: 119 DLIWTQCKPCTQCFDQPTPIFDPKKSSSFSKLSCSSKLCEALPQSTCSDGCEYLYGYGDY 178

Query: 122 SSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQLD 181
           SST+G LA ETLT G    +VS P +AFGCG  N   G  QG+G+VGL RGPLSL+SQL+
Sbjct: 179 SSTQGILASETLTFG----KVSVPKVAFGCGEDNEGSGFSQGSGLVGLGRGPLSLVSQLE 238

Query: 182 IHKFSYCLT--------------------------------------------------- 241
             KFSYCLT                                                   
Sbjct: 239 EPKFSYCLTSVDDTKASTLLMGSLASVKASDSEIKTTPLIQNSAQPSFYYLSLEGISVGD 298

Query: 242 -------------PNGTGSLNLESSTSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAG 301
                         +G+G L ++S T+I        + AFD++   FT+Q NLPV +S  
Sbjct: 299 TSLPIKKSTFSLQEDGSGGLIIDSGTTITYLE----QSAFDLVAKEFTSQINLPVDNSGS 358

Query: 302 IGLDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSI 325
            GL++CF LPS   + ++VP LVFHF+G +L+LP ENYM+ D   G+ CLAMG++  +SI
Sbjct: 359 TGLEVCFTLPS-GSTDIEVPKLVFHFDGADLELPPENYMIADASMGVACLAMGSSSGMSI 418

[Close]

BLAST of Spg008651 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 277.3 bits (708), Expect = 1.5e-74
Identity = 165/402 (41.04%), Postives = 220/402 (54.73%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAI--------SNFDVEMPVVERKRSYLMNLSLRMPPVSFSAI 61
           ++RGI R   R+     +A+           +++ P       +LM LS+  P V +SAI
Sbjct: 64  IQRGINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAI 123

Query: 62  ADTGSDLIWTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSS---SSNGCE 121
            DTGSDLIWTQCKPC +CF+QP+P++DP +SSS++    S+ LC AL  S+     + CE
Sbjct: 124 VDTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNEDKDACE 183

Query: 122 YSVGYGDGSSTKGYLAFETLTLGEANQQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGP 181
           Y   YGD SST+G LA ET T  + N   S   I FGCG++N   G  QG+G+VGL RGP
Sbjct: 184 YLYTYGDYSSTRGLLATETFTFEDEN---SISGIGFGCGVENEGDGFSQGSGLVGLGRGP 243

Query: 182 LSLISQLDIHKFSYCLTP--------------------NGTGS----------------- 241
           LSLISQL   KFSYCLT                     N TG+                 
Sbjct: 244 LSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPD 303

Query: 242 ------LNLESST------SIDNTTNRAIKD-------------------AFDVLKPFFT 301
                 L L+  T      S++ +T    +D                   AF VLK  FT
Sbjct: 304 QPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFT 363

Query: 302 AQTNLPVKHSAGIGLDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLV 325
           ++ +LPV  S   GLDLCF+LP   K+ + VP ++FHF+G +L+LP ENYMV D   G++
Sbjct: 364 SRMSLPVDDSGSTGLDLCFKLPDAAKN-IAVPKMIFHFKGADLELPGENYMVADSSTGVL 423

[Close]

BLAST of Spg008651 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 181.4 bits (459), Expect = 1.2e-45
Identity = 124/388 (31.96%), Postives = 181/388 (46.65%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAISNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGSDLI 61
           LR  I R   R+ +F     +    ++ +      YLMN+S+  PP    AIADTGSDL+
Sbjct: 56  LRNAIHRSVNRVFHFTEKD-NTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLL 115

Query: 62  WTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTN----SSSSNGCEYSVGYGD 121
           WTQC PC  C+ Q  P++DP  SS++ + + S++ C AL N    S++ N C YS+ YGD
Sbjct: 116 WTQCAPCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGD 175

Query: 122 GSSTKGYLAFETLTLGEAN-QQVSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQ 181
            S TKG +A +TLTLG ++ + +   +I  GCG  N     K+G+G+VGL  GP+SLI Q
Sbjct: 176 NSYTKGNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQ 235

Query: 182 LDIH---KFSYCLTP--------------------------------------------- 241
           L      KFSYCL P                                             
Sbjct: 236 LGDSIDGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKS 295

Query: 242 ----------NGTGSLNLESSTSIDNTTNRAI--KDAFDVLKPFFTAQTNLPVKHSAGIG 301
                     +G+ S + E +  ID+ T   +   + +  L+    +  +   K     G
Sbjct: 296 ISVGSKQIQYSGSDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSG 355

Query: 302 LDLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSIFG 325
           L LC+         L VP +  HF+G ++KL   N  V   E  LVC A   + + SI+G
Sbjct: 356 LSLCYSA----TGDLKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIYG 415

[Close]

BLAST of Spg008651 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 168.7 bits (426), Expect = 7.8e-42
Identity = 129/387 (33.33%), Postives = 185/387 (47.80%), Query Frame = 0


Query: 2   LRRGIERDKTRIQNFNMMAISNFDVEMPVVERKRSYLMNLSLRMPPVSFSAIADTGSDLI 61
           +R  I R       F+    S    +  +   +  YLMN+S+  PPV   AIADTGSDLI
Sbjct: 51  MRNAIRRSARSTLQFSNDDASPNSPQSFITSNRGEYLMNISIGTPPVPILAIADTGSDLI 110

Query: 62  WTQCKPCLQCFEQPSPVYDPARSSSFANATSSAALCKALTNSSSS---NGCEYSVGYGDG 121
           WTQC PC  C++Q SP++DP  SS++   + S++ C+AL ++S S   N C Y++ YGD 
Sbjct: 111 WTQCNPCEDCYQQTSPLFDPKESSTYRKVSCSSSQCRALEDASCSTDENTCSYTITYGDN 170

Query: 122 SSTKGYLAFETLTLGEANQQ-VSAPDIAFGCGLKNHVKGLKQGAGMVGLSRGPLSLISQL 181
           S TKG +A +T+T+G + ++ VS  ++  GCG +N       G+G++GL  G  SL+SQL
Sbjct: 171 SYTKGDVAVDTVTMGSSGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQL 230

Query: 182 --DIH-KFSYCLTP---------------NGTGS-------------------LNLES-- 241
              I+ KFSYCL P               NG  S                   LNLE+  
Sbjct: 231 RKSINGKFSYCLVPFTSETGLTSKINFGTNGIVSGDGVVSTSMVKKDPATYYFLNLEAIS 290

Query: 242 --STSIDNTTNRAIKDAFDVLKPFFTAQTNLP-------------------VKHSAGIGL 301
             S  I  T+        +++    T  T LP                   V+   GI L
Sbjct: 291 VGSKKIQFTSTIFGTGEGNIVIDSGTTLTLLPSNFYYELESVVASTIKAERVQDPDGI-L 350

Query: 302 DLCFELPSHNKSKLDVPDLVFHFEGLNLKLPVENYMVTDEERGLVCLAMGAAGALSIFGS 325
            LC+     + S   VPD+  HF+G ++KL   N  V   E  + C A  A   L+IFG+
Sbjct: 351 SLCY----RDSSSFKVPDITVHFKGGDVKLGNLNTFVAVSE-DVSCFAFAANEQLTIFGN 410

[Close]

BLAST of Spg008651 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 148.3 bits (373), Expect = 1.1e-35
Identity = 111/362 (30.66%), Postives = 164/362 (45.30%), Query Frame = 0


Query: 25  DVEMPVV----ERKRSYLMNLSLRMPPVSFSAIADTGSDLIWTQCKPCLQCFEQPSPVYD 84
           D+  PVV    +    Y   + +  P      + DTGSD+ W QC+PC  C++Q  PV++
Sbjct: 146 DLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFN 205

Query: 85  PARSSSFANATSSAALCKAL-TNSSSSNGCEYSVGYGDGSSTKGYLAFETLTLGEANQQV 144
           P  SS++ + T SA  C  L T++  SN C Y V YGDGS T G LA +T+T G + +  
Sbjct: 206 PTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSGK-- 265

Query: 145 SAPDIAFGCGLKNHVKGLKQG-AGMVGLSRGPLSLISQLDIHKFSYCLTPNGTG---SLN 204
              ++A GCG  N  +GL  G AG++GL  G LS+ +Q+    FSYCL    +G   SL+
Sbjct: 266 -INNVALGCGHDN--EGLFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLD 325

Query: 205 LES--------------STSID-------------------------------------- 264
             S              +  ID                                      
Sbjct: 326 FNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDC 385

Query: 265 -NTTNRAIKDAFDVLKPFFTAQTNLPVKHSAGIGL-DLCFELPSHNKSKLDVPDLVFHFE 322
                R    A++ L+  F   T    K S+ I L D C++  S   S + VP + FHF 
Sbjct: 386 GTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSS--LSTVKVPTVAFHFT 445

[Close]

BLAST of Spg008651 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.4e-35
Identity = 109/365 (29.86%), Postives = 164/365 (44.93%), Query Frame = 0


Query: 25  DVEMPVV----ERKRSYLMNLSLRMPPVSFSAIADTGSDLIWTQCKPCLQCFEQPSPVYD 84
           D+E P++    +    Y   + +  P      + DTGSD+ W QC PC  C+ Q  P+++
Sbjct: 132 DIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFE 191

Query: 85  PARSSSFANATSSAALCKALTNSSSSNG-CEYSVGYGDGSSTKGYLAFETLTLGEANQQV 144
           P+ SSS+   +     C AL  S   N  C Y V YGDGS T G  A ETLT+G    Q 
Sbjct: 192 PSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVSYGDGSYTVGDFATETLTIGSTLVQ- 251

Query: 145 SAPDIAFGCGLKNHVKGLKQG-AGMVGLSRGPLSLISQLDIHKFSYCLT----------- 204
              ++A GCG  N  +GL  G AG++GL  G L+L SQL+   FSYCL            
Sbjct: 252 ---NVAVGCGHSN--EGLFVGAAGLLGLGGGLLALPSQLNTTSFSYCLVDRDSDSASTVD 311

Query: 205 ------------------------------------------------PNGTGSLNLESS 264
                                                            +G+G + ++S 
Sbjct: 312 FGTSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQIPQSSFEMDESGSGGIIIDSG 371

Query: 265 TSIDNTTNRAIKDAFDVLKPFFTAQTNLPVKHSAGIGL-DLCFELPSHNKSKLDVPDLVF 322
           T++     R   + ++ L+  F   T L ++ +AG+ + D C+ L +  K+ ++VP + F
Sbjct: 372 TAV----TRLQTEIYNSLRDSFVKGT-LDLEKAAGVAMFDTCYNLSA--KTTVEVPTVAF 431

The following BLAST results are available for this feature:

BLAST of Spg008651 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886455.1	2.8e-134	67.28	aspartic proteinase nepenthesin-2-like [Benincasa hispida]	[more]
KAG6737285.1	4.5e-84	44.36	hypothetical protein POTOM_058799 [Populus tomentosa]	[more]
XP_011046330.1	7.7e-84	44.10	PREDICTED: aspartic proteinase nepenthesin-1-like [Populus euphratica]	[more]
XP_006370905.1	1.0e-83	44.10	aspartic proteinase nepenthesin-1 [Populus trichocarpa] >PNS89732.1 hypothetical...	[more]
XP_034919474.1	1.0e-83	44.10	aspartic proteinase nepenthesin-1-like [Populus alba] >TKS10217.1 aspartyl prote...	[more]

BLAST of Spg008651 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q766C3	2.9e-70	40.67	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	5.9e-63	38.22	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q6XBF8	1.6e-44	31.96	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q7XV21	1.3e-38	29.45	Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2	[more]
Q9LS40	1.5e-34	30.66	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]

BLAST of Spg008651 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2N9HF56	8.0e-87	47.41	Peptidase A1 domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4...	[more]
A0A6M2F5X8	9.8e-85	44.62	Peptidase A1 domain-containing protein OS=Populus davidiana OX=266767 PE=3 SV=1	[more]
B9N2J4	4.9e-84	44.10	Peptidase A1 domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_0...	[more]
A0A4U5QLX0	4.9e-84	44.10	Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000085760 PE...	[more]
A0A4U5R6H0	8.3e-84	44.10	Aspartyl protease family protein OS=Populus alba OX=43335 GN=D5086_0000004600 PE...	[more]

BLAST of Spg008651 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G03200.1	1.5e-74	41.04	Eukaryotic aspartyl protease family protein	[more]
AT5G33340.1	1.2e-45	31.96	Eukaryotic aspartyl protease family protein	[more]
AT1G64830.1	7.8e-42	33.33	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	1.1e-35	30.66	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	2.4e-35	29.86	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 37..195 e-value: 1.4E-44 score: 152.4
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 17..192 e-value: 5.7E-48 score: 165.4
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 193..325 e-value: 7.3E-22 score: 79.7
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 35..324
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 2..191
None	No IPR available	PANTHER	PTHR47967:SF23	OS08G0469000 PROTEIN	coord: 2..191 coord: 210..323
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 210..323
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 37..326 score: 16.906471
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 37..321 e-value: 3.62372E-66 score: 207.115

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg008651.1	Spg008651.1	mRNA