HG10000564 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10000564
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
LocationChr09: 6489238 .. 6491306 (+)
RNA-Seq ExpressionHG10000564
SyntenyHG10000564
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCCAAAGCTTTTGGCTTTACATATCCATGCTTCTGAGTTTGCTGGCAATGTTATCATCATATGGGAGTTGCAGCTTTGACAGTGGATTCTCCATTGAAGAAGCAACTTTGAAAGATCTCCAACTTGCTTTCTACCAAAACAAACTCACCTCCAAGCAACTTGTCAAGTTTTACCTCAAACAAGTGCGTAGATTTAACCCCATTCTGAAAGGGATCATAGAAGTGAATCCAGATGCTTTACACCAAGCCTCTCGAGCCGACCTCGAGCGCAAGAGAAACGCCCCGATCACTTTGTCTCCATTGCATGGCATTCCTGTACTTCTAAAAGATAACATTGGAACCAAGGATAAACTCAACACAACGGCTGGCTCTTTTGCTCTGCTTGGCTCTATTGTTCCTCGTGATGCAGGGGTGGTGACGAAGTTGAGGAAGGCAGGTGCAATCATCTTTGGAAAAGCGAGCCTGAGCGAGTGGTCTTATTTCAGGTCCTATAGTCTCCCCAATGGTTGGTGCGCTAGGAGTGGCCAAGGCAAGGTCTGTAACTGTCCATCAATCTTATAAATGTTGTGAGGTCTTTTGATTTTTCTAATGTAGGATCCTCAACATACCCCTCTCTTCTGGGTTACCATTCTTGACCCAAAAGCTTAAGTTGATGAGTTATGGTAAATTTAATTATATCAACACTTTAACATATATCACTAATCCTCTAACTTTTAAAACAAAACAAGTTAGAAAGACCAATTAAACGACAACAAACTAAGAACACTCGTATGCACCATGTTTACAGAATCCTTACACATTGGGAGAACCTTGTGGCTCGAGTAGCGGCTCTGCCATATCTGTTGCAGCAAATATGGTTGCAGTTTCACTAGGAACTGACACTGATGGATCAATATTATGCCCTTCTACTTTCAATTCAGTAGTTGGCATTAGGCCAACAGTAGGTCTCACCAGTCGAGCAGGGGTTGTTCCAATCTCTTTGAGGCAGGATGCTATTGGGTAATTAATTTGTTTCTATTCAAATCTCGCCATTTATATTGATATCCAAAGGAAGTACTTTCTGCTGAATGAGTAATGTATGAACAGGCCTATTTGTAGAACAGTATCAGATGCTGCTTATGTTCTAGATGCAATTGTAGGAGCGGACAGATATGATAATTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGATATGGCCAATTTCTAAGGGCTGATAGGCTGAAAGGAAAGAGAATAGGAATCGCGAGGAAATTCTTTGATTTTGGCCATGATGATGTCTTCTACACAGGAGCTTTTGAGAAAGTTTTCAAAGCCTTGAAGTAAGAAAGAAAACTCACTTTTCATAAACCATTTCTTGATAATTCATGTTAGATGTTTTTATCTGACCCTTCAGCTTCAATAACATGAAAGGCAAGGAGGAGCAATATTGGTGGACAATCTGACAATAGAGAGCCTAGACATAATCATTGGTGGTTTAAGTGGGGAGTGGACTGCATTGCCTGCCGAATTCAAAATATCCTTTAATGCATACCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTGTCAGATGCAATAGAATTCAACAAAAAGAACTCTAAACTTGTGAGCAATTATCATCTATTCTGTTCTAATTTTACAATCAGCACAGCAAAAAGCAGACTATCATAACAATGAAATCTTATCACTATGTAGGAAAAGCTAAAGGAGTATGGTCAGGAGTTATTTCTAAAAGCAGAAGCTACAAAAGGAATTGGAGTTGTAGAAAAGGCAGCATTAACCAGATTAGCAACACTGTCAAAAAAGGGATTTGAGAGAGTAATGATTAAGAATAAGCTTGATGCAATAGCAGCTCCTGGTTGGGCAATATCTCCTGTTCTTGCAATTGGAGGTTTTCCTGGAATTAGTGTACCAGCTGGATATGACCCTGAAGGAGTTCCCTATGGCATCGGCTTTGGAGGATTAAAGGGGTTTGAGCCAAGGCTGATAGAGATAGCATATGGATTTGAGCATTTGACTAAGAGTAGAAAGTCTCCTTCAATGAAGAGAAGTTAA

mRNA sequence

ATGGCTGCCCAAAGCTTTTGGCTTTACATATCCATGCTTCTGAGTTTGCTGGCAATGTTATCATCATATGGGAGTTGCAGCTTTGACAGTGGATTCTCCATTGAAGAAGCAACTTTGAAAGATCTCCAACTTGCTTTCTACCAAAACAAACTCACCTCCAAGCAACTTGTCAAGTTTTACCTCAAACAAGTGCGTAGATTTAACCCCATTCTGAAAGGGATCATAGAAGTGAATCCAGATGCTTTACACCAAGCCTCTCGAGCCGACCTCGAGCGCAAGAGAAACGCCCCGATCACTTTGTCTCCATTGCATGGCATTCCTGTACTTCTAAAAGATAACATTGGAACCAAGGATAAACTCAACACAACGGCTGGCTCTTTTGCTCTGCTTGGCTCTATTGTTCCTCGTGATGCAGGGGTGGTGACGAAGTTGAGGAAGGCAGGTGCAATCATCTTTGGAAAAGCGAGCCTGAGCGAGTGGTCTTATTTCAGGTCCTATAGTCTCCCCAATGGTTGGTGCGCTAGGAGTGGCCAAGGCAAGAATCCTTACACATTGGGAGAACCTTGTGGCTCGAGTAGCGGCTCTGCCATATCTGTTGCAGCAAATATGGTTGCAGTTTCACTAGGAACTGACACTGATGGATCAATATTATGCCCTTCTACTTTCAATTCAGTAGTTGGCATTAGGCCAACAGTAGGTCTCACCAGTCGAGCAGGGGTTGTTCCAATCTCTTTGAGGCAGGATGCTATTGGGCCTATTTGTAGAACAGTATCAGATGCTGCTTATGTTCTAGATGCAATTGTAGGAGCGGACAGATATGATAATTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGATATGGCCAATTTCTAAGGGCTGATAGGCTGAAAGGAAAGAGAATAGGAATCGCGAGGAAATTCTTTGATTTTGGCCATGATGATGTCTTCTACACAGGAGCTTTTGAGAAAGTTTTCAAAGCCTTGAAGCAAGGAGGAGCAATATTGGTGGACAATCTGACAATAGAGAGCCTAGACATAATCATTGGTGGTTTAAGTGGGGAGTGGACTGCATTGCCTGCCGAATTCAAAATATCCTTTAATGCATACCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTGTCAGATGCAATAGAATTCAACAAAAAGAACTCTAAACTTGAAAAGCTAAAGGAGTATGGTCAGGAGTTATTTCTAAAAGCAGAAGCTACAAAAGGAATTGGAGTTGTAGAAAAGGCAGCATTAACCAGATTAGCAACACTGTCAAAAAAGGGATTTGAGAGAGTAATGATTAAGAATAAGCTTGATGCAATAGCAGCTCCTGGTTGGGCAATATCTCCTGTTCTTGCAATTGGAGGTTTTCCTGGAATTAGTGTACCAGCTGGATATGACCCTGAAGGAGTTCCCTATGGCATCGGCTTTGGAGGATTAAAGGGGTTTGAGCCAAGGCTGATAGAGATAGCATATGGATTTGAGCATTTGACTAAGAGTAGAAAGTCTCCTTCAATGAAGAGAAGTTAA

Coding sequence (CDS)

ATGGCTGCCCAAAGCTTTTGGCTTTACATATCCATGCTTCTGAGTTTGCTGGCAATGTTATCATCATATGGGAGTTGCAGCTTTGACAGTGGATTCTCCATTGAAGAAGCAACTTTGAAAGATCTCCAACTTGCTTTCTACCAAAACAAACTCACCTCCAAGCAACTTGTCAAGTTTTACCTCAAACAAGTGCGTAGATTTAACCCCATTCTGAAAGGGATCATAGAAGTGAATCCAGATGCTTTACACCAAGCCTCTCGAGCCGACCTCGAGCGCAAGAGAAACGCCCCGATCACTTTGTCTCCATTGCATGGCATTCCTGTACTTCTAAAAGATAACATTGGAACCAAGGATAAACTCAACACAACGGCTGGCTCTTTTGCTCTGCTTGGCTCTATTGTTCCTCGTGATGCAGGGGTGGTGACGAAGTTGAGGAAGGCAGGTGCAATCATCTTTGGAAAAGCGAGCCTGAGCGAGTGGTCTTATTTCAGGTCCTATAGTCTCCCCAATGGTTGGTGCGCTAGGAGTGGCCAAGGCAAGAATCCTTACACATTGGGAGAACCTTGTGGCTCGAGTAGCGGCTCTGCCATATCTGTTGCAGCAAATATGGTTGCAGTTTCACTAGGAACTGACACTGATGGATCAATATTATGCCCTTCTACTTTCAATTCAGTAGTTGGCATTAGGCCAACAGTAGGTCTCACCAGTCGAGCAGGGGTTGTTCCAATCTCTTTGAGGCAGGATGCTATTGGGCCTATTTGTAGAACAGTATCAGATGCTGCTTATGTTCTAGATGCAATTGTAGGAGCGGACAGATATGATAATTCAACAATTGAAGCATCAAAATACATTCCAAAAGGTGGATATGGCCAATTTCTAAGGGCTGATAGGCTGAAAGGAAAGAGAATAGGAATCGCGAGGAAATTCTTTGATTTTGGCCATGATGATGTCTTCTACACAGGAGCTTTTGAGAAAGTTTTCAAAGCCTTGAAGCAAGGAGGAGCAATATTGGTGGACAATCTGACAATAGAGAGCCTAGACATAATCATTGGTGGTTTAAGTGGGGAGTGGACTGCATTGCCTGCCGAATTCAAAATATCCTTTAATGCATACCTCAAAGAGCTAGTTGCTTCTCCAATTCGATCCTTGTCAGATGCAATAGAATTCAACAAAAAGAACTCTAAACTTGAAAAGCTAAAGGAGTATGGTCAGGAGTTATTTCTAAAAGCAGAAGCTACAAAAGGAATTGGAGTTGTAGAAAAGGCAGCATTAACCAGATTAGCAACACTGTCAAAAAAGGGATTTGAGAGAGTAATGATTAAGAATAAGCTTGATGCAATAGCAGCTCCTGGTTGGGCAATATCTCCTGTTCTTGCAATTGGAGGTTTTCCTGGAATTAGTGTACCAGCTGGATATGACCCTGAAGGAGTTCCCTATGGCATCGGCTTTGGAGGATTAAAGGGGTTTGAGCCAAGGCTGATAGAGATAGCATATGGATTTGAGCATTTGACTAAGAGTAGAAAGTCTCCTTCAATGAAGAGAAGTTAA

Protein sequence

MAAQSFWLYISMLLSLLAMLSSYGSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVKFYLKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQGKNPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLKGKRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGVVEKAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVPYGIGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSMKRS
Homology
BLAST of HG10000564 vs. NCBI nr
Match: XP_038900913.1 (probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 881.7 bits (2277), Expect = 3.0e-252
Identity = 457/514 (88.91%), Postives = 482/514 (93.77%), Query Frame = 0

Query: 1   MAAQSFWLYISMLLSLLAMLSSYGSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVKFY 60
           MAAQSFWLYISM+LSL+A+LSSYG+CSF + F IEEAT+KDLQLAFYQNKLTS+QLVKFY
Sbjct: 1   MAAQSFWLYISMVLSLVAILSSYGNCSFGTHFPIEEATVKDLQLAFYQNKLTSRQLVKFY 60

Query: 61  LKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKDKL 120
           LKQVRRFNPILKGIIEVNPDAL QA+RAD ERKR++  +LSPLHGIPVLLKDNI TKDKL
Sbjct: 61  LKQVRRFNPILKGIIEVNPDALDQATRADHERKRSSLSSLSPLHGIPVLLKDNIATKDKL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRS +LPNGWCAR GQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSQFRS-NLPNGWCARGGQGK 180

Query: 181 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 240
           NPYT+GEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPST+NSVVGIRPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTYNSVVGIRPTVGLTSRAGV 240

Query: 241 VPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLKG 300
           VPIS RQDA+GPICRTVSDAAYVLDAIVGAD+YDNSTIEASKYIPKGGYGQFLR D LKG
Sbjct: 241 VPISSRQDAVGPICRTVSDAAYVLDAIVGADKYDNSTIEASKYIPKGGYGQFLRPDGLKG 300

Query: 301 KRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWTAL 360
            RIGI RKFFDFG D VFY+ A+EKVFK+LKQGGAILVDNLTI+SLDIIIGGLSGEWTAL
Sbjct: 301 TRIGIVRKFFDFGDDGVFYSEAYEKVFKSLKQGGAILVDNLTIDSLDIIIGGLSGEWTAL 360

Query: 361 PAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGVVE 420
           PAEFKIS NAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQ LFL+AEAT GIG VE
Sbjct: 361 PAEFKISINAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQVLFLEAEATNGIGDVE 420

Query: 421 KAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVPYG 480
           KAAL  +A+LSK GFERVMIKNKLDAIAAPG  ISPVLAIGGFPGISVPAGY P+GVPYG
Sbjct: 421 KAALASVASLSKNGFERVMIKNKLDAIAAPGGLISPVLAIGGFPGISVPAGYSPDGVPYG 480

Query: 481 IGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSMKR 515
           IGFGGLKGFEPRLIEIAYGFE LTKSRK P +KR
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEQLTKSRKPPLIKR 513

BLAST of HG10000564 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 856.7 bits (2212), Expect = 1.0e-244
Identity = 438/514 (85.21%), Postives = 471/514 (91.63%), Query Frame = 0

Query: 1   MAAQSFWLYISMLLSLLAMLSSYGSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVKFY 60
           MAAQSF +YISMLL LLA+LSSYGSCSFD+ FSIEEATLKDLQLAFYQNKLTS QLV+FY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKDKL 120
           L+QVRRFNPIL GIIEVNPDAL+QAS+ADLERKR++P +LSPLHGIPVL+KDNI TKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY  PNGW AR GQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 240
           NPYT+GEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLKG 300
           VPISLRQD +GPICRTV+DAAYVLDAI G DRYDNSTIEASKY+PKGGYGQFL+ D LKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWTAL 360
           KRIGI RK +DFGHDDVFY GAFEKVFK LKQGGAILVDNLTI S  +I G  SGEWTA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 PAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGVVE 420
            AEFKIS N YLK+LVASPIRSLSDAIEFN+KNSKLEKLKEYGQELFL+AEATKGIG  E
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 KAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVPYG 480
           KAAL RLA LSK+GFER+MIKNKLDAIAAPG  IS  LAIGGFPG+SVPAGY+P+G+P+G
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 481 IGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSMKR 515
           IGFGGLKGFEPRLIEIAYGFEHLT  RKSPS+ R
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of HG10000564 vs. NCBI nr
Match: XP_038900912.1 (LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 856.3 bits (2211), Expect = 1.4e-244
Identity = 436/514 (84.82%), Postives = 472/514 (91.83%), Query Frame = 0

Query: 1   MAAQSFWLYISMLLSLLAMLSSYGSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVKFY 60
           MAAQSFWLYISMLLSLLA+LSSYGSCSFD+  SIEEAT+KDLQLAFYQNKL S+QLVKFY
Sbjct: 6   MAAQSFWLYISMLLSLLAILSSYGSCSFDTHLSIEEATVKDLQLAFYQNKLISRQLVKFY 65

Query: 61  LKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKDKL 120
           L  VRRFNPILKGIIEVNPDAL QAS+ADLERKRN+P +LSPLHGIPVL+KDNI T+DKL
Sbjct: 66  LNXVRRFNPILKGIIEVNPDALDQASQADLERKRNSPSSLSPLHGIPVLVKDNIATRDKL 125

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLS+WSYFRSY +P+GW AR GQGK
Sbjct: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSKWSYFRSYXIPSGWSARGGQGK 185

Query: 181 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 240
           NPYT+GEPCGSS GSAIS+AANMVAVSLGT+TDGSILCPS  NSVVGI+PTVGLTSRAGV
Sbjct: 186 NPYTMGEPCGSSGGSAISIAANMVAVSLGTETDGSILCPSILNSVVGIKPTVGLTSRAGV 245

Query: 241 VPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLKG 300
           +PISLRQD +GPICRTVSD AYVL AIVGAD+ DNSTIEASKYIP+GGY QFLRAD LKG
Sbjct: 246 IPISLRQDTVGPICRTVSDDAYVLGAIVGADKNDNSTIEASKYIPRGGYAQFLRADGLKG 305

Query: 301 KRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWTAL 360
           KRIGI RKFFDFGHDD FY  A+EKVFK L+QGGA+ VDNLTI+SL +I+G  SGE TAL
Sbjct: 306 KRIGIVRKFFDFGHDDAFYPRAYEKVFKTLRQGGAVFVDNLTIDSLQVIMGSSSGEETAL 365

Query: 361 PAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGVVE 420
            AEFKIS NAYLK+LVASPIRSLSDAIEFN+KNSKLEKL+EYGQ LF++AEATKGIG  E
Sbjct: 366 LAEFKISLNAYLKQLVASPIRSLSDAIEFNEKNSKLEKLREYGQNLFIEAEATKGIGDAE 425

Query: 421 KAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVPYG 480
           KAAL RLA LSK GFERVM+KNKLDAIAAPGW IS VLAIGGFPGISVPAGY+ +GVP+G
Sbjct: 426 KAALARLAKLSKDGFERVMLKNKLDAIAAPGWEISSVLAIGGFPGISVPAGYNSQGVPFG 485

Query: 481 IGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSMKR 515
           I FGGLKGFEPRLIEIAYGFEHLTKSRKSPS+KR
Sbjct: 486 ISFGGLKGFEPRLIEIAYGFEHLTKSRKSPSIKR 519

BLAST of HG10000564 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 851.3 bits (2198), Expect = 4.4e-243
Identity = 438/516 (84.88%), Postives = 472/516 (91.47%), Query Frame = 0

Query: 1   MAAQSFWLYISMLLSLLAMLSSY--GSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVK 60
           MA QSF +YISMLL LLA+LS Y  GSCSFD+ FSIEEATLKD QLAFYQNKLTS+QLV+
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKD 120
           FYL+QVRR NPILKGIIEVNPDAL+QAS+ADL+RKR++  +LSPLHGIPVL+KDNI TKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY  PNGW AR GQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRA 240
           GKNPYT+GEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRL 300
           GVVPISLRQD +GPICRTV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWT 360
           KGKRIGI RK +DFGHDDVFY GAFEKVFK LKQGGAILVDNLTI   D+I G  SGEWT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGV 420
           AL AEFKIS NAYLK+LVASPIRSLSDAIEFNKKNSKLEKL+EYGQELFL+AEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 VEKAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVP 480
            EKAAL RLA LSK+GFER+MIKNKLDAIAAPG  ISP LAIGGFPG+SVPAGY+P+G+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 YGIGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSMKR 515
           +GIGFGGLKGF+PRLIEIAYGFEHLT  RKSPS+ R
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of HG10000564 vs. NCBI nr
Match: ADN34007.1 (amidase [Cucumis melo subsp. melo])

HSP 1 Score: 839.0 bits (2166), Expect = 2.3e-239
Identity = 430/505 (85.15%), Postives = 463/505 (91.68%), Query Frame = 0

Query: 12  MLLSLLAMLSSY--GSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVKFYLKQVRRFNP 71
           MLL LLA+LS Y  GSCSFD+ FSIEEATLKD QLAFYQNKLTS+QLV+FYL+QVRR NP
Sbjct: 1   MLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNP 60

Query: 72  ILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKDKLNTTAGSFAL 131
           ILKGIIEVNPDAL+QAS+ADL+RKR++  +LSPLHGIPVL+KDNI TKDKLNTTAGSFAL
Sbjct: 61  ILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL 120

Query: 132 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQGKNPYTLGEPC 191
           LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY  PNGW AR GQGKNPYT+GEPC
Sbjct: 121 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEPC 180

Query: 192 GSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDA 251
           GSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGVVPISLRQD 
Sbjct: 181 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT 240

Query: 252 IGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLKGKRIGIARKF 311
           +GPICRTV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ LKGKRIGI RK 
Sbjct: 241 VGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRKL 300

Query: 312 FDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWTALPAEFKISFN 371
           +DFGHDDVFY GAFEKVFK LKQGGAILVDNLTI   D+I G  SGEWTAL AEFKIS N
Sbjct: 301 YDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWTALLAEFKISLN 360

Query: 372 AYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGVVEKAALTRLAT 431
           AYLK+LVASPIRSLSDAIEFNKKNSKLEKL+EYGQELFL+AEATKGIG  EKAAL RLA 
Sbjct: 361 AYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAALARLAK 420

Query: 432 LSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVPYGIGFGGLKGF 491
           LSK+GFER+MIKNKLDAIAAPG  ISP LAIGGFPG+SVPAGY+P+G+P+GIGFGGLKGF
Sbjct: 421 LSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGFGGLKGF 480

Query: 492 EPRLIEIAYGFEHLTKSRKSPSMKR 515
           +PRLIEIAYGFEHLT  RKSPS+ R
Sbjct: 481 DPRLIEIAYGFEHLTMGRKSPSLGR 505

BLAST of HG10000564 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 4.8e-160
Identity = 307/518 (59.27%), Postives = 391/518 (75.48%), Query Frame = 0

Query: 1   MAAQSFWLYISMLLSLL---AMLSSYGSCS---FDSGFSIEEATLKDLQLAFYQNKLTSK 60
           M A  F+ + S+L+ L+   A++ S GS S     S FSI+EAT++D+++AF + +LTSK
Sbjct: 1   MVALRFFFFSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSK 60

Query: 61  QLVKFYLKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNI 120
           QLV+ YL+ + + NPIL  +IE NPDAL QA  AD ER       L  LHG+PVLLKD+I
Sbjct: 61  QLVELYLEAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSI 120

Query: 121 GTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCA 180
            TKDKLNTTAGSFALLGS+V RDAGVV +LR++GA+I GKASLSEW++FRS+S+P+GW A
Sbjct: 121 STKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA 180

Query: 181 RSGQGKNPYTL-GEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVG 240
           R  QGKNPY L   P GSSSGSAISV AN+VAVSLGT+TDGSIL P++ NSVVGI+P+VG
Sbjct: 181 RGLQGKNPYVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVG 240

Query: 241 LTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFL 300
           LTSRAGVVPISLRQD+IGPICRTVSDA ++LDAIVG D  D +T  AS++IP+GGY QFL
Sbjct: 241 LTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFL 300

Query: 301 RADRLKGKRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGL 360
               LKGKR+GI  K          ++   +   K L++ GAI+++NLTI ++++I+GG 
Sbjct: 301 TTSGLKGKRLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGT 360

Query: 361 -SGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEA 420
            SGE  AL AEFK+S NAYLKELV SP+RSL+D I +N++ ++ EK+KE+GQE+FL AEA
Sbjct: 361 DSGEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEA 420

Query: 421 TKGIGVVEKAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGY 480
           T G+G  EK AL ++  LS+ G E+++ +NKLDAI   G  +S VLAIGG+PGI+VPAGY
Sbjct: 421 TSGMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGY 480

Query: 481 DPEGVPYGIGFGGLKGFEPRLIEIAYGFEHLTKSRKSP 511
           D  GVPYGI FGGL+  EP+LIEIA+ FE  T  RK P
Sbjct: 481 DSGGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of HG10000564 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 5.7e-76
Identity = 197/513 (38.40%), Postives = 283/513 (55.17%), Query Frame = 0

Query: 33  SIEEATLKDLQLAFYQNKLTSKQLVKFYLKQVRRFNPILKGIIEVNPDALHQASRADLER 92
           ++E+AT+  LQ       LTS  +V  YL +  + NP + GI+++NPD L  AS  D ER
Sbjct: 65  TLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDER 124

Query: 93  KRNAPITLSPLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIF 152
                I   PLHGIP ++KDN  TKDK++TTAGS+ALLGSIVPRDA VV +LR+AGA++F
Sbjct: 125 ANG--IIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVLF 184

Query: 153 GKASLSEWSYFRSYSLPNGWCARSGQGKNPYTLG-EPCGSSSGSAISVAANMVAVSLGTD 212
           G A+LSEW+  RS     G+ AR GQ + P+ L   P GSSSGSAISVA+NM+A +LGT+
Sbjct: 185 GHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGTE 244

Query: 213 TDGSILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGAD 272
           TDGSI+ P+  N VVG++PTVGLTSR GV+P S  QD  GPI RTV DA YV  ++ G D
Sbjct: 245 TDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGID 304

Query: 273 RYDNSTIEASKYIPK-GGYGQFL-RADRLKGKRIGI--ARKFFDFGHDDVFYTGAFEKVF 332
             D  T+  +   P+ G Y +FL     L+G R G+   R + +   D++       +V 
Sbjct: 305 ENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEI---DRLLEVV 364

Query: 333 KALKQGGAILVDNLTIESLDII--------IGGLS-GEWTALPAEFKISFNAYLKELVAS 392
           K +++ GAI+ +N    +LD+I        +G ++  E+T +  +F  +  +YL E+  +
Sbjct: 365 KQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKNT 424

Query: 393 PIRSLSDAIEFNKKNSKLEKLK-------EYGQELFLKAEATKGI--GVVEKAALTRLAT 452
            I SL D +E+N K    E  K         GQ+ FL +    G+      +A      T
Sbjct: 425 EIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRRT 484

Query: 453 LSKKGFERVMIKNKLDAIAAPGWAISPVL------------AIGGFPGISVPAGYDPEGV 511
              +G +  +  N  D      + ++ +L            A  G+P I++P G    G 
Sbjct: 485 SQDEGIDYAL--NYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGR 544

BLAST of HG10000564 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.2e-63
Identity = 173/495 (34.95%), Postives = 259/495 (52.32%), Query Frame = 0

Query: 42  LQLAFYQNKLTSKQLVKFYLKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLS 101
           LQ  + Q  +    +V+ Y+ ++   N  ++ + E+NPDAL  A + D ERK        
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGK--LRG 153

Query: 102 PLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS 161
           PLHG+P+++K+NI T DK+++TAGS+A+ G+    DA V TKLR+AG +I GK+  S+W+
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 162 YFRSYSLPNGWCARSGQGKNPYTLG-EPCGSSSGSAISVAANMVAVSLGTDTDGSILCPS 221
            FRS +  NGW A  GQ    Y    +P GSSSGS ++    +   +LGT+T GSI+ P+
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 222 TFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEA 281
             +++VG++PTVGLTSR  VVPIS RQD +GP+ R+V DAAY+L  I G D  DN T  A
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-SA 333

Query: 282 SKYIPKGGYGQFLRADRLKGKRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDN 341
             +     Y +    + LKGKRIG+ R               F +    +K+ GAI+V+N
Sbjct: 334 IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAIIVEN 393

Query: 342 LTIESLDIIIGGLSGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLK 401
               S          +   L A+   +  A+ K+L  +P  +++D +E  ++ ++  +L+
Sbjct: 394 TDFTSFAEFAQSPIPD-DILYADSLTNLPAFFKQLKVNP-HNITD-LESLRRFTQHHRLE 453

Query: 402 EYGQELFLKAEAT--KGIGVVEKAALTRLATLSKKGFERVMI----KNKLDAIAAPGWAI 461
           EY      + +    KGI   +           K G E  ++    ++KLDA   P    
Sbjct: 454 EYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTDLS 513

Query: 462 SPVLAIGGFPGISVPAGYDPE---------------GVPYGIGFGGLKGFEPRLIEIAYG 515
             + A+ G P I+VP G  P                G+P GIGF G    E +LI +AY 
Sbjct: 514 PYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLAYA 573

BLAST of HG10000564 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.1e-42
Identity = 165/514 (32.10%), Postives = 251/514 (48.83%), Query Frame = 0

Query: 38  TLKDLQLAFYQNKLTSKQLVKFYLKQVRRFNPILKGIIEVNPD-ALHQASRADLERKRNA 97
           T+ +L+    + ++++ ++ K YL++++   P +  +I +  D AL +A  AD E+ +N 
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQRAKEAD-EKIKNG 66

Query: 98  PITLSPLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKAS 157
             T   L GIPV++KDNI T + + TT  S  L   I P +A VV KL + G II GK++
Sbjct: 67  EDT--ALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSN 126

Query: 158 LSEWSYFRSYSLPNGWCARSGQGKNPYTLGE-PCGSSSGSAISVAANMVAVSLGTDTDGS 217
           L E++     S  N     +   KNP+ L   P GSS GSA ++AA+  A +LG+DT GS
Sbjct: 127 LDEFA--MGSSTENSAFKTT---KNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGS 186

Query: 218 ILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDN 277
           I  P++   VVG++PT GL SR G+V  +   D IGP  + V+D A VL+ I+G D  D+
Sbjct: 187 IRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDS 246

Query: 278 STIEASKYIPKGGYGQFLRADRLKGKRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGA 337
           ++++    I K  Y  +L+ D +KG RIG+A++FF  G ++    G  E V +++K    
Sbjct: 247 TSLK----IDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEE----GVKETVQESIK---- 306

Query: 338 ILVDNLTIESLDIIIGGLSGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSK 397
            ++ +L  E +DI I  +     ALPA + I+         AS   +  D I +     K
Sbjct: 307 -VLQDLGAEIIDISIPYVE---YALPAYYIIA------SAEASSNLARYDGIRYGHIAGK 366

Query: 398 LEKL-------------KEYGQELFLKAEATKG--IGVVEKAALTRLATLSKKGFERVMI 457
            E L             KE  + + L   A          K AL ++ TL K  FE+   
Sbjct: 367 YEDLIDMYMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKAL-KVRTLIKNDFEKAF- 426

Query: 458 KNKLDAIAAPGWAISPVLA------------------------IGGFPGISVPAGYDPEG 511
             K D I  P    SP +A                        I G PGIS+P G   +G
Sbjct: 427 -EKCDVIIGP---TSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLS-DG 481

BLAST of HG10000564 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.4e-42
Identity = 162/514 (31.52%), Postives = 250/514 (48.64%), Query Frame = 0

Query: 38  TLKDLQLAFYQNKLTSKQLVKFYLKQVRRFNPILKGIIEVNPD-ALHQASRADLERKRNA 97
           T+ +L+    + ++++ ++ K YL++++   P +  ++ +  D AL +A  AD + K+  
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIKKGE 66

Query: 98  PITLSPLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKAS 157
              L+   GIPV++KDNI T + + TT  S  L   I P +A VV KL + G II GK++
Sbjct: 67  DTALT---GIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSN 126

Query: 158 LSEWSYFRSYSLPNGWCARSGQGKNPYTLGE-PCGSSSGSAISVAANMVAVSLGTDTDGS 217
           L E++     S  N     +   KNP+ L   P GSS GSA ++AA+  A +LG+DT GS
Sbjct: 127 LDEFA--MGSSTENSAFKTT---KNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGS 186

Query: 218 ILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDN 277
           I  P++   VVG++PT GL SR G+V  +   D IGP  + V+D A VL+ I+G D  D+
Sbjct: 187 IRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDS 246

Query: 278 STIEASKYIPKGGYGQFLRADRLKGKRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGA 337
           ++++    I K  Y  +L+ D +KG RIG+A++FF  G ++    G  E V +++K    
Sbjct: 247 TSLK----IDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEE----GVKETVQESIK---- 306

Query: 338 ILVDNLTIESLDIIIGGLSGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSK 397
            ++ +L  E +DI I  +     ALPA + I+         AS   +  D I +     K
Sbjct: 307 -VLQDLGAEIIDISIPYVE---YALPAYYIIA------SAEASSNLARYDGIRYGHIAEK 366

Query: 398 LEKL-------------KEYGQELFLKAEATKG--IGVVEKAALTRLATLSKKGFERVMI 457
            E L             KE  + + L   A          K AL ++ TL K  FE+   
Sbjct: 367 YEDLIDMYMVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKAL-KVRTLIKNDFEKAF- 426

Query: 458 KNKLDAIAAPGWAISPVLA------------------------IGGFPGISVPAGYDPEG 511
             K D I  P    SP +A                        I G PGIS+P G   +G
Sbjct: 427 -EKCDVIIGP---TSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLS-DG 481

BLAST of HG10000564 vs. ExPASy TrEMBL
Match: A0A0A0LLK0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=4 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.5e-244
Identity = 437/514 (85.02%), Postives = 470/514 (91.44%), Query Frame = 0

Query: 1   MAAQSFWLYISMLLSLLAMLSSYGSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVKFY 60
           MAAQSF +YISMLL LLA+LSSYGSCSFD+ FSIEEATLKDLQLAFYQNKLTS QLV+FY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  LKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKDKL 120
           L+QVRRFNPIL GIIEVNPDAL+QAS+ADLERKR++P +LSPLHGIPV +KDNI TKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVFVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY  PNGW AR GQGK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGV 240
           NPYT+GEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLKG 300
           VPISLRQD +GPICRTV+DAAYVLDAI G DRYDNSTIEASKY+PKGGYGQFL+ D LKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWTAL 360
           KRIGI RK +DFGHDDVFY GAFEKVFK LKQGGAILVDNLTI S  +I G  SGEWTA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 PAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGVVE 420
            AEFKIS N YLK+LVASPIRSLSDAIEFN+KNSKLEKLKEYGQELFL+AEATKGIG  E
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 KAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVPYG 480
           KAAL RLA LSK+GFER+MIKNKLDAIAAPG  IS  LAIGGFPG+SVPAGY+P+G+P+G
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 481 IGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSMKR 515
           IGFGGLKGFEPRLIEIAYGFEHLT  RKSPS+ R
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of HG10000564 vs. ExPASy TrEMBL
Match: A0A5D3BN38 (Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001380 PE=4 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 2.1e-243
Identity = 438/516 (84.88%), Postives = 472/516 (91.47%), Query Frame = 0

Query: 1   MAAQSFWLYISMLLSLLAMLSSY--GSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVK 60
           MA QSF +YISMLL LLA+LS Y  GSCSFD+ FSIEEATLKD QLAFYQNKLTS+QLV+
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKD 120
           FYL+QVRR NPILKGIIEVNPDAL+QAS+ADL+RKR++  +LSPLHGIPVL+KDNI TKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY  PNGW AR GQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRA 240
           GKNPYT+GEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRL 300
           GVVPISLRQD +GPICRTV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWT 360
           KGKRIGI RK +DFGHDDVFY GAFEKVFK LKQGGAILVDNLTI   D+I G  SGEWT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGV 420
           AL AEFKIS NAYLK+LVASPIRSLSDAIEFNKKNSKLEKL+EYGQELFL+AEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 VEKAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVP 480
            EKAAL RLA LSK+GFER+MIKNKLDAIAAPG  ISP LAIGGFPG+SVPAGY+P+G+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 YGIGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSMKR 515
           +GIGFGGLKGF+PRLIEIAYGFEHLT  RKSPS+ R
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of HG10000564 vs. ExPASy TrEMBL
Match: A0A1S3C7A8 (putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 2.1e-243
Identity = 438/516 (84.88%), Postives = 472/516 (91.47%), Query Frame = 0

Query: 1   MAAQSFWLYISMLLSLLAMLSSY--GSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVK 60
           MA QSF +YISMLL LLA+LS Y  GSCSFD+ FSIEEATLKD QLAFYQNKLTS+QLV+
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKD 120
           FYL+QVRR NPILKGIIEVNPDAL+QAS+ADL+RKR++  +LSPLHGIPVL+KDNI TKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY  PNGW AR GQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRA 240
           GKNPYT+GEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRL 300
           GVVPISLRQD +GPICRTV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWT 360
           KGKRIGI RK +DFGHDDVFY GAFEKVFK LKQGGAILVDNLTI   D+I G  SGEWT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGV 420
           AL AEFKIS NAYLK+LVASPIRSLSDAIEFNKKNSKLEKL+EYGQELFL+AEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 VEKAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVP 480
            EKAAL RLA LSK+GFER+MIKNKLDAIAAPG  ISP LAIGGFPG+SVPAGY+P+G+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 YGIGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSMKR 515
           +GIGFGGLKGF+PRLIEIAYGFEHLT  RKSPS+ R
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of HG10000564 vs. ExPASy TrEMBL
Match: E5GC08 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 1.1e-239
Identity = 430/505 (85.15%), Postives = 463/505 (91.68%), Query Frame = 0

Query: 12  MLLSLLAMLSSY--GSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVKFYLKQVRRFNP 71
           MLL LLA+LS Y  GSCSFD+ FSIEEATLKD QLAFYQNKLTS+QLV+FYL+QVRR NP
Sbjct: 1   MLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNP 60

Query: 72  ILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKDKLNTTAGSFAL 131
           ILKGIIEVNPDAL+QAS+ADL+RKR++  +LSPLHGIPVL+KDNI TKDKLNTTAGSFAL
Sbjct: 61  ILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKDKLNTTAGSFAL 120

Query: 132 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQGKNPYTLGEPC 191
           LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY  PNGW AR GQGKNPYT+GEPC
Sbjct: 121 LGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEPC 180

Query: 192 GSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDA 251
           GSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRAGVVPISLRQD 
Sbjct: 181 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDT 240

Query: 252 IGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRLKGKRIGIARKF 311
           +GPICRTV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ LKGKRIGI RK 
Sbjct: 241 VGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRKL 300

Query: 312 FDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWTALPAEFKISFN 371
           +DFGHDDVFY GAFEKVFK LKQGGAILVDNLTI   D+I G  SGEWTAL AEFKIS N
Sbjct: 301 YDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWTALLAEFKISLN 360

Query: 372 AYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGVVEKAALTRLAT 431
           AYLK+LVASPIRSLSDAIEFNKKNSKLEKL+EYGQELFL+AEATKGIG  EKAAL RLA 
Sbjct: 361 AYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAALARLAK 420

Query: 432 LSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVPYGIGFGGLKGF 491
           LSK+GFER+MIKNKLDAIAAPG  ISP LAIGGFPG+SVPAGY+P+G+P+GIGFGGLKGF
Sbjct: 421 LSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGFGGLKGF 480

Query: 492 EPRLIEIAYGFEHLTKSRKSPSMKR 515
           +PRLIEIAYGFEHLT  RKSPS+ R
Sbjct: 481 DPRLIEIAYGFEHLTMGRKSPSLGR 505

BLAST of HG10000564 vs. ExPASy TrEMBL
Match: A0A1S3C652 (putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 9.3e-239
Identity = 434/516 (84.11%), Postives = 468/516 (90.70%), Query Frame = 0

Query: 1   MAAQSFWLYISMLLSLLAMLSSY--GSCSFDSGFSIEEATLKDLQLAFYQNKLTSKQLVK 60
           MA QSF +YISMLL LLA+LS Y  GSCSFD+ FSIEEATLKD QLAFYQNKLTS+QLV+
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  FYLKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKD 120
           FYL+QVRR NPILKGIIEVNPDAL+QAS+ADL+RKR++  +LSPLHGIPVL+KDNI TKD
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQ 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSY  PNGW AR GQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRA 240
           GKNPYT+GEPCGSSSGSAISVAANMV VSLGT+TDGSILCPST NSVVGI+PTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADRL 300
           GVVPISLRQD +G    TV+DAAYVLDAI GADRYDNSTIEASKYIP+GGYGQFLRA+ L
Sbjct: 241 GVVPISLRQDTVG----TVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 KGKRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWT 360
           KGKRIGI RK +DFGHDDVFY GAFEKVFK LKQGGAILVDNLTI   D+I G  SGEWT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGV 420
           AL AEFKIS NAYLK+LVASPIRSLSDAIEFNKKNSKLEKL+EYGQELFL+AEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 VEKAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYDPEGVP 480
            EKAAL RLA LSK+GFER+MIKNKLDAIAAPG  ISP LAIGGFPG+SVPAGY+P+G+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 YGIGFGGLKGFEPRLIEIAYGFEHLTKSRKSPSMKR 515
           +GIGFGGLKGF+PRLIEIAYGFEHLT  RKSPS+ R
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 512

BLAST of HG10000564 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 486.9 bits (1252), Expect = 2.0e-137
Identity = 273/517 (52.80%), Postives = 353/517 (68.28%), Query Frame = 0

Query: 1   MAAQSFWLYISMLLSLL---AMLSSYGSCS---FDSGFSIEEATLKDLQLAFYQNKLTSK 60
           M A  F+ + S+L+ L+   A++ S GS S     S FSI+EAT++D+++AF + +LTSK
Sbjct: 1   MVALRFFFFSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSK 60

Query: 61  QLVKFYLKQVRRFNPILKGIIEVNPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNI 120
           QLV+ YL+ + + NPIL  +IE NPDAL QA  AD ER       L  LHG+PVLLKD+I
Sbjct: 61  QLVELYLEAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSI 120

Query: 121 GTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCA 180
            TKDKLNTTAGSFALLGS+V RDAGVV +LR++GA+I GKASLSEW++FRS+S+P+GW A
Sbjct: 121 STKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA 180

Query: 181 RSGQGKNPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGL 240
            S                                              NSVVGI+P+VGL
Sbjct: 181 PSQ---------------------------------------------NSVVGIKPSVGL 240

Query: 241 TSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLR 300
           TSRAGVVPISLRQD+IGPICRTVSDA ++LDAIVG D  D +T  AS++IP+GGY QFL 
Sbjct: 241 TSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLT 300

Query: 301 ADRLKGKRIGIARKFFDFGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGL- 360
              LKGKR+GI  K          ++   +   K L++ GAI+++NLTI ++++I+GG  
Sbjct: 301 TSGLKGKRLGIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTD 360

Query: 361 SGEWTALPAEFKISFNAYLKELVASPIRSLSDAIEFNKKNSKLEKLKEYGQELFLKAEAT 420
           SGE  AL AEFK+S NAYLKELV SP+RSL+D I +N++ ++ EK+KE+GQE+FL AEAT
Sbjct: 361 SGEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEAT 420

Query: 421 KGIGVVEKAALTRLATLSKKGFERVMIKNKLDAIAAPGWAISPVLAIGGFPGISVPAGYD 480
            G+G  EK AL ++  LS+ G E+++ +NKLDAI   G  +S VLAIGG+PGI+VPAGYD
Sbjct: 421 SGMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYD 462

Query: 481 PEGVPYGIGFGGLKGFEPRLIEIAYGFEHLTKSRKSP 511
             GVPYGI FGGL+  EP+LIEIA+ FE  T  RK P
Sbjct: 481 SGGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of HG10000564 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 125.6 bits (314), Expect = 1.2e-28
Identity = 83/232 (35.78%), Postives = 126/232 (54.31%), Query Frame = 0

Query: 50  KLTSKQLVKFYLKQVRRFNPILKGIIEVNPD-ALHQASRADLERKRNAPITLSPLHGIPV 109
           ++TSK+LV+ YLKQ++R+N +L+ ++    + A  QA  AD    +     L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGT--YLGPLHGIPY 275

Query: 110 LLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSL 169
            LKD +       TT GS +     +  +A V  +L+ +GA++  K      +Y      
Sbjct: 276 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAY------ 335

Query: 170 PNGWCARSGQGKNPYTLGE-PCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVG 229
            + W    G+ +NP+ + E   GSS+G A S +A MV  ++G++T GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCGITA 395

Query: 230 IRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIE 280
           +RPT G   R GV+ IS   D +GP CRT +D A +LDAI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 436

BLAST of HG10000564 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 124.4 bits (311), Expect = 2.6e-28
Identity = 143/529 (27.03%), Postives = 238/529 (44.99%), Query Frame = 0

Query: 19  MLSSYGSCSFD-SGFSIEEATLKDLQLAFYQNKLTSKQLVKFYLKQVRRFNPILKGIIEV 78
           ++S+  S + D S  S  ++ +   + +    + T+ ++ K YL ++R   P LK  + V
Sbjct: 25  IVSAASSKTIDTSVISPPQSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHV 84

Query: 79  NPDALHQASRADLERKRNAPITLSPLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRD 138
           + + L  A   D    +     L PL G+ + +KDNI T+  + +TA S  L     P D
Sbjct: 85  SENVLKDAQEIDQRIAKGE--ELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFD 144

Query: 139 AGVVTKLRKAGAIIFGKASLSEWSYFRSYSLPNGWCARSGQGKNPYTLGE-PCGSSSGSA 198
           A  V K+++ G I+ GK ++ E+    +        +      NP+ L   P GSS GSA
Sbjct: 145 ATAVKKIKELGGIVVGKTNMDEFGMGSTTE-----ASAFQVTANPWDLSRVPGGSSGGSA 204

Query: 199 ISVAANMVAVSLGTDTDGSILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRT 258
            +VAA    VSLG+DT GS+  P++F  VVG++PT G  SR G++  +   D IG    T
Sbjct: 205 AAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGST 264

Query: 259 VSDAAYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRADR-----LKGKRIGIARKFFD 318
           V+DA  +L AI G DR+D++   +SK        QFL  D      L G ++GI R+  +
Sbjct: 265 VADAGMLLHAISGYDRFDST---SSKQDVPEFQSQFLSVDHFESKPLNGVKVGIIRETLE 324

Query: 319 FGHDDVFYTGAFEKVFKALKQGGAILVDNLTIESLDIIIGGLSGEWTALPAEFKISFNAY 378
            G D      A ++    L+  G IL + +++ S  +           LPA + I+ +  
Sbjct: 325 DGVDS-GVRSATQEAASHLEALGCILTE-VSLPSFSL----------GLPAYYVIASSES 384

Query: 379 LKELVASPIRSLSDAIEFNKK--NSKLEKLKE------YGQELFLKA-----EATKGIGV 438
              L      S  D + +  +    +L KL E      +G E+ ++        + G   
Sbjct: 385 SSNL------SRYDGVRYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTYALSAGYYD 444

Query: 439 VEKAALTRLATLSKKGFERVMIKNK-LDAIAAP------------------GWAISPVLA 498
                  ++ TL +K F+  + +N  L + AAP                  G  ++  + 
Sbjct: 445 AYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVN 504

Query: 499 IGGFPGISVPAGY---DPEGVPYGIGFGGLKGFEPRLIEIAYGFEHLTK 506
           + G P + +P G     P G+P G+   G    E +L+++ + FE   K
Sbjct: 505 LAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLK 524

BLAST of HG10000564 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 114.8 bits (286), Expect = 2.1e-25
Identity = 81/232 (34.91%), Postives = 122/232 (52.59%), Query Frame = 0

Query: 50  KLTSKQLVKFYLKQVRRFNPILKGIIEVNPD-ALHQASRADLERKRNAPITLSPLHGIPV 109
           ++TSK+LV+ YLKQ++R+N +L+ ++    + A  QA  AD    +     L PLHGIP 
Sbjct: 216 QITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGT--YLGPLHGIPY 275

Query: 110 LLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSYFRSYSL 169
            LKD +       TT GS +     +  +A V  +L+ +GA++  K      +Y      
Sbjct: 276 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAY------ 335

Query: 170 PNGWCARSGQGKNPYTLGE-PCGSSSGSAISVAANMVAVSLGTDTDGSILCPSTFNSVVG 229
            + W    G+ +NP+ + E   GSS+G A S +A       G++T GS+  P+    +  
Sbjct: 336 DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAARCGITA 395

Query: 230 IRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYDNSTIE 280
           +RPT G   R GV+ IS   D +GP CRT +D A +LDAI G D  D S+ E
Sbjct: 396 LRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 429

BLAST of HG10000564 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 105.9 bits (263), Expect = 9.7e-23
Identity = 130/494 (26.32%), Postives = 206/494 (41.70%), Query Frame = 0

Query: 39  LKDLQLAFYQNKLTSKQLVKFYLKQVRRF---NPILKGIIEVNPDALHQASRADLER-KR 98
           ++D   A+     T  Q+ K  +  +  F    P    +I  + + + + + A   R ++
Sbjct: 131 IRDYAYAYRSKLTTPLQVAKRIISIIEEFGYDKPPTPFLIRFDANEVIKQAEASTRRFEQ 190

Query: 99  NAPITLSPLHGIPVLLKDNIGTKDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGK 158
             PI  S L GI V +KD+I           ++      V +D+ VV+KLR  GAI+ GK
Sbjct: 191 GNPI--SVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGK 250

Query: 159 ASLSEWSYFRSYSLPNGWCARSGQGKNPYTLGEPCGSSSGSAISVAANMVAVSLGTDTDG 218
           A++ E     + +  N    R+      YT     GSSSGSA  VAA + + +LGTD  G
Sbjct: 251 ANMHELGMGTTGNNSNYGTTRNPHDPKRYT----GGSSSGSAAIVAAGLCSAALGTDGGG 310

Query: 219 SILCPSTFNSVVGIRPTVGLTSRAGVVPISLRQDAIGPICRTVSDAAYVLDAIVGADRYD 278
           S+  PS    + G++ T G T   G +      + IGP+  ++ DA  V  AI+G+   D
Sbjct: 311 SVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLVYAAILGSSSAD 370

Query: 279 NSTIEAS-----KYIPKGGYGQFLRADRLKGKRIGIARKFF-DFGHDDVFYTGAFEKVFK 338
              ++ S     K +   G      ++ +   R+G   K+F D    D+  +   E + K
Sbjct: 371 RYNLKPSPPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDVSSSDI--SDKCEDILK 430

Query: 339 ALKQGGAILVDNLTIESLD-------IIIGGLS-GEWTALPAEFKISFNAYLKELVASPI 398
            L       V  + +  L+       I IG  +    T      K S  +Y      +  
Sbjct: 431 LLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKNSKLSYDTRTSFAIF 490

Query: 399 RSLSDAIEFNKKNSKLEKLKEYGQELFLKAEATKGIGVVEKAALTRLATLSKKGFERVMI 458
           RS S A ++        +L EY   +F      K + V+    +T    ++        +
Sbjct: 491 RSFS-ASDYIAAQCLRRRLMEYHLNIF------KDVDVI----VTPTTGMTAPVIPPDAL 550

Query: 459 KNKLDAIAAPGWAISPVLAIG--GFPGISVPAGYDPEGVPYGIGFGGLKGFEPRLIEIAY 513
           KN    I      +  VLA    GFP ISVP GYD EG+P G+   G    E  ++ +A 
Sbjct: 551 KNGETNIQVTTDLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGLAA 599

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900913.13.0e-25288.91probable amidase At4g34880 [Benincasa hispida][more]
XP_004147024.31.0e-24485.21probable amidase At4g34880 [Cucumis sativus][more]
XP_038900912.11.4e-24484.82LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida][more]
XP_008457659.14.4e-24384.88PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 puta... [more]
ADN34007.12.3e-23985.15amidase [Cucumis melo subsp. melo][more]
Match NameE-valueIdentityDescription
A0A1P8B7604.8e-16059.27Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY45.7e-7638.40Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C83.2e-6334.95Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
B0KBN41.1e-4232.10Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanol... [more]
B0K3S31.4e-4231.52Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain ... [more]
Match NameE-valueIdentityDescription
A0A0A0LLK01.5e-24485.02Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=... [more]
A0A5D3BN382.1e-24384.88Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3C7A82.1e-24384.88putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4... [more]
E5GC081.1e-23985.15Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A1S3C6529.3e-23984.11putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4... [more]
Match NameE-valueIdentityDescription
AT4G34880.12.0e-13752.80Amidase family protein [more]
AT5G07360.11.2e-2835.78Amidase family protein [more]
AT3G25660.12.6e-2827.03Amidase family protein [more]
AT5G07360.22.1e-2534.91Amidase family protein [more]
AT5G64440.19.7e-2326.32fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 56..457
e-value: 2.7E-69
score: 234.3
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 25..512
e-value: 1.9E-139
score: 467.9
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 33..510
NoneNo IPR availablePANTHERPTHR42678:SF19C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 17..513
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 17..513

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10000564.1HG10000564.1mRNA