HG10008538 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10008538 vs. NCBI nr
Match: XP_038878698.1 (flavonoid 3'-monooxygenase [Benincasa hispida])

HSP 1 Score: 920.2 bits (2377), Expect = 7.8e-264
Identity = 466/519 (89.79%), Postives = 482/519 (92.87%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           MDEI+LIVAA VVTLA  ILLHR FF R RF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1   MDEIALIVAA-VVTLASTILLHRTFFRRSRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAGEYG LMYLRLGSV VVVAASA+VAE+LLK+H TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGEYGSLMYLRLGSVGVVVAASAAVAEKLLKVHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEI VLVQALA SGE PA LGSLLTVCTA
Sbjct: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIAVLVQALASSGEVPAALGSLLTVCTA 180

Query: 181 NVLGRTMISRRVFGD-GSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGD GSG D EA  FKAMVEQVM+L GKFNPGDFFPSLEWLDLLGVAS
Sbjct: 181 NALGRTMIGRRVFGDGGSGDDSEAKQFKAMVEQVMILAGKFNPGDFFPSLEWLDLLGVAS 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIK 300
           EMKKVHKWFDDFLTKIVEEHRNLLA G   GG QDLLSTLLSS   AEDENEK+ND+EIK
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGRG-GGHQDLLSTLLSS---AEDENEKINDTEIK 300

Query: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 360
           ALLLNMFTAGTDTSASTVEWALTELIRHPK+MA+AQ+ELD+VVGRDRAVSDLDL QL YL
Sbjct: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKMMAQAQQELDSVVGRDRAVSDLDLPQLVYL 360

Query: 361 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 420
           QAVVKETFRLHPPTPLS+PRMAAESCEINGYHIPKG+TLLVD+WAI RDPKEWADPLEFR
Sbjct: 361 QAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGTTLLVDVWAIGRDPKEWADPLEFR 420

Query: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNG 480
           PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATL H FDWALPNG
Sbjct: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLVHYFDWALPNG 480

Query: 481 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 519
           LLPD+LNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQK P
Sbjct: 481 LLPDELNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKSP 514

BLAST of HG10008538 vs. NCBI nr
Match: XP_008453263.1 (PREDICTED: flavonoid 3'-monooxygenase [Cucumis melo] >KAA0057987.1 flavonoid 3'-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 904.0 bits (2335), Expect = 5.8e-259
Identity = 452/522 (86.59%), Postives = 479/522 (91.76%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+EI+LIVAA ++TLA A LLHR FFLRRRF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1   MEEITLIVAA-IITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKIS VHLFSSKALDDHRRIR++EI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 180

Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGDGSG D  E+N FKAMVEQVMVL GKFNPGDFFP LE LDL+GV S
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLA---GGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           EMKKVHKWFDDFLTKIVEEHRNLLA   GG   GG QDLLSTLLS KDE  DENEKLND+
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD+VVGRDRAVSDLDL QL
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPKEW DPL
Sbjct: 361 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
           EF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDWAL
Sbjct: 421 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 519
           PNGL PDKLNMDE YGLTLRRAQPLVVHPRPRLL GVYQKLP
Sbjct: 481 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of HG10008538 vs. NCBI nr
Match: XP_004138192.1 (flavonoid 3'-monooxygenase [Cucumis sativus] >KGN63725.2 hypothetical protein Csa_014030 [Cucumis sativus])

HSP 1 Score: 889.0 bits (2296), Expect = 1.9e-254
Identity = 441/517 (85.30%), Postives = 474/517 (91.68%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+EI+ IVAA ++TLA A LLHRAFFLRRR  LPPGPKPWPIVGNMPQIG A LHSLLA 
Sbjct: 1   MEEITPIVAA-LITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLAT 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIA 180

Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGDGSG D  E+N FK MVEQVMVL GKFNPGDFFP LEWLDL+GV  
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGR 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIK 300
           EMKKVHKWFDDFLTKIVEEHRNLLA G   GG QDLLSTLLS KD+ EDENEKLND+EIK
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGVG-GGHQDLLSTLLSMKDDGEDENEKLNDTEIK 300

Query: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 360
           ALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD++VGRDRAVSD+DL QL YL
Sbjct: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSIVGRDRAVSDVDLHQLVYL 360

Query: 361 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 420
           QAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPK+W DPLEFR
Sbjct: 361 QAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFR 420

Query: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNG 480
           PNRFLP GEK HVDVKGNDFEVIPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDW LPNG
Sbjct: 421 PNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNG 480

Query: 481 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQK 517
           L PDKLNMDE YGLTLRRAQPL++HPRPRLL GVY++
Sbjct: 481 LTPDKLNMDEHYGLTLRRAQPLIMHPRPRLLLGVYER 515

BLAST of HG10008538 vs. NCBI nr
Match: XP_023529494.1 (flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo] >XP_023529495.1 flavonoid 3'-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 870.2 bits (2247), Expect = 9.3e-249
Identity = 433/521 (83.11%), Postives = 470/521 (90.21%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
           MDEISL  +A  +TLA  +LL+R  F +R      LPPGPKPWPIVGN+PQIGGAPLHSL
Sbjct: 1   MDEISLFFSA-AITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
           LA M+GEYGPLMYLR GSVDVVVAASA++AEQ LKI  TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61  LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
           VFAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+GVLVQALARSGEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180

Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
           CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPGDFFPSL+WLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFPSLDWLDLLG 240

Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGG--DQDLLSTLLSSKDEAEDENEKLN 300
           VA EMKKVHKWFDD L+KI+EEHRNLLA GG  GG   QDLLSTLLS++D AEDENEKLN
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRNLLASGGGGGGGEHQDLLSTLLSARDRAEDENEKLN 300

Query: 301 DSEIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLS 360
           D+EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD++VGRDR VSDLDL 
Sbjct: 301 DTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMHQAQQELDSIVGRDRVVSDLDLP 360

Query: 361 QLPYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWAD 420
           +L YLQAVVKETFRLHPPTPLS+PRMAAESCEI+GY IPKGSTLLVD+WAISRDPKEW  
Sbjct: 361 KLGYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYRIPKGSTLLVDVWAISRDPKEWTS 420

Query: 421 PLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDW 480
           PLEFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGLRMVQ+LTA+L HSFDW
Sbjct: 421 PLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQILTASLVHSFDW 480

Query: 481 ALPNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 516
           ALP+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 ALPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 520

BLAST of HG10008538 vs. NCBI nr
Match: XP_022933611.1 (flavonoid 3'-monooxygenase [Cucurbita moschata])

HSP 1 Score: 865.9 bits (2236), Expect = 1.7e-247
Identity = 432/519 (83.24%), Postives = 468/519 (90.17%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
           MDEISL  +A  +TLA  +LL+R  F +R      LPPGPKPWPIVGN+PQIGGAPLHSL
Sbjct: 1   MDEISLFFSA-AITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
           LA M+GEYGPLMYLR GSVDVVVAASA++AEQ LKI  TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61  LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
           VFAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+GVLVQALARSGEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180

Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
           CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPGDFF  LEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLG 240

Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           VA EMKKVHKWFDD ++KI+EEHRNLLA GG  G  QDLLSTLLS++D A+DENEKLND+
Sbjct: 241 VAGEMKKVHKWFDDLMSKILEEHRNLLATGGG-GEHQDLLSTLLSTRDRAKDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD+VVGRDR VSDLDL +L
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMAAESCEI+GYHIPKGSTLLVD+WAISRDPKEW  PL
Sbjct: 361 GYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
           EFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGLRMVQMLTA+L HSFDWAL
Sbjct: 421 EFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 516
           P+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 PSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of HG10008538 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 1.0e-189
Identity = 328/516 (63.57%), Postives = 406/516 (78.68%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+ +SLI+  V+ +     +L   F  R    LPPGPKPWPI+GN+  +G  P H   AA
Sbjct: 1   MEILSLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKP-HQSTAA 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MA  YGPLMYL++G VDVVVAASASVA Q LK H  NF SRPPN+GA+++AYN +D+VFA
Sbjct: 61  MAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWR+LRKI +VHLFS+KALDD R +R++E+  L +ALA +G+ P  LG LL VCT 
Sbjct: 121 PYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVCTT 180

Query: 181 NVLGRTMISRRVFGDGSGG-DPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N L R M+ +RVF DGSG  DP+A  FK+MV ++MV+ G FN GDF P L WLD+ GVA+
Sbjct: 181 NALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAA 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSK-DEAEDENEKLNDSEI 300
           +MKK+H  FD FLT I+EEH+     G   G  +DLLSTL+S K D+A+++  KL D+EI
Sbjct: 241 KMKKLHARFDAFLTDILEEHK-----GKIFGEMKDLLSTLISLKNDDADNDGGKLTDTEI 300

Query: 301 KALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPY 360
           KALLLN+F AGTDTS+STVEWA+ ELIR+PKI+A+AQ+E+D VVGRDR V +LDL+QL Y
Sbjct: 301 KALLLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTY 360

Query: 361 LQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEF 420
           L+A+VKETFRLHP TPLS+PR+A+ESCEINGY IPKGSTLL+++WAI+RDP  WADPLEF
Sbjct: 361 LEAIVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEF 420

Query: 421 RPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPN 480
           RP RFLPGGEK  VDV+GNDFEVIPFGAGRRIC GM++G+RMVQ++ ATL H+F+W L +
Sbjct: 421 RPERFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVS 480

Query: 481 GLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
           G LP+ LNM+EAYGLTL+RA PLVVHPRPRL    Y
Sbjct: 481 GQLPEMLNMEEAYGLTLQRADPLVVHPRPRLEAQAY 510

BLAST of HG10008538 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.8e-183
Identity = 318/515 (61.75%), Postives = 396/515 (76.89%), Query Frame = 0

Query: 5   SLIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAA 64
           +L +  ++ T+ + IL  R F  RR       LPPGP PWPI+GN+P +G  P H  L+A
Sbjct: 3   TLFLTILLATVLFLIL--RIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSA 62

Query: 65  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 124
           M   YGP+++LRLG VDVVVAAS SVAEQ LKIH  NF SRPPN+GAK++AYN +D+VFA
Sbjct: 63  MVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFA 122

Query: 125 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 184
           PYG RWRLLRKIS+VHLFS+KAL+D + +R+EE+G L + L R G  P  LG L+ +C  
Sbjct: 123 PYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVV 182

Query: 185 NVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASE 244
           N LGR MI RR+F  G+  D +A+ F++MV ++M L G FN GDF PSL+WLDL GVA +
Sbjct: 183 NALGREMIGRRLF--GADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGK 242

Query: 245 MKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSK-DEAEDENEKLNDSEIK 304
           MK++HK FD FL+ I++EH       G      D+LSTL+S K  + + +   L D+EIK
Sbjct: 243 MKRLHKRFDAFLSSILKEHEM----NGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIK 302

Query: 305 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 364
           ALLLNMFTAGTDTSASTV+WA+ ELIRHP IM +AQ+ELD VVGRDR V++ D++QLPYL
Sbjct: 303 ALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYL 362

Query: 365 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 424
           QAV+KE FRLHPPTPLS+P +A+ESCEINGYHIPKGSTLL +IWAI+RDP +W+DPL F+
Sbjct: 363 QAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFK 422

Query: 425 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNG 484
           P RFLPGGEK+ VDVKG+DFE+IPFGAGRRIC G+S+GLR +Q LTATL   FDW L  G
Sbjct: 423 PERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGG 482

Query: 485 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
           + P+KLNM+E+YGLTL+RA PLVVHP+PRL P VY
Sbjct: 483 VTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of HG10008538 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 1.3e-165
Identity = 293/516 (56.78%), Postives = 373/516 (72.29%), Query Frame = 0

Query: 5   SLIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAA 64
           +L++++ +V L Y          RRR      LPPGP  WPI+GN+PQ+G  P H  L A
Sbjct: 11  TLLLSSFIVYLLY----------RRRSAQCPPLPPGPNGWPILGNLPQLGAKP-HQTLDA 70

Query: 65  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 124
           ++ +YGPL  LRLGSV+VVVA+S++VA Q L+ H  NF +RPPN+GA+++AYN +D+VFA
Sbjct: 71  LSKQYGPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFA 130

Query: 125 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAP--ATLGSLLTVC 184
           PYGPRWR+LRK+ +VHLFS KALDD R +R+ E+  LV+ L R  +      LG  L VC
Sbjct: 131 PYGPRWRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVC 190

Query: 185 TANVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVA 244
             N L R M+ RRVF D      EA+ FK MV ++M L G FN GDF P L WLDL GV 
Sbjct: 191 ATNALARAMLGRRVFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVV 250

Query: 245 SEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEI 304
            +MK++H+ +D FL +++EE++     G       DLLS L+  K+   +   KLN+++I
Sbjct: 251 GKMKRLHRRYDAFLDRVIEENQANAKSG-------DLLSVLIRLKEADAEGEIKLNNTDI 310

Query: 305 KALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPY 364
           KALLLN+FTAGTDTS+STVEW L ELIRHP I+ + Q ELD+V+GRDR V++ DL  LPY
Sbjct: 311 KALLLNLFTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPY 370

Query: 365 LQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEF 424
           LQAVVKETFRLHP TPLS+PRMA+E C +NGY IPK +TLLV++W+I RD   W DPLEF
Sbjct: 371 LQAVVKETFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEF 430

Query: 425 RPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPN 484
           RP+RFLPGGE+ HVDVKGNDFEVIPFGAGRRIC G+S+GLRMVQ +TAT+ H++DW+LP 
Sbjct: 431 RPSRFLPGGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPK 490

Query: 485 GLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
           G    KL+M+EAYGLTL+RA PL+V P PRL    Y
Sbjct: 491 GQECQKLDMEEAYGLTLQRAVPLMVQPIPRLSHKAY 508

BLAST of HG10008538 vs. ExPASy Swiss-Prot
Match: Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 6.2e-163
Identity = 284/493 (57.61%), Postives = 358/493 (72.62%), Query Frame = 0

Query: 28  RRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEYGPLMYLRLGSVDVVVAASASVA 87
           R+R  LPPGP+ WP++GN+PQ+G  P H  + A+A +YGPL  LR G  +VVVAASA VA
Sbjct: 36  RKRRPLPPGPRGWPVLGNLPQLGDKP-HHTMCALARQYGPLFRLRFGCAEVVVAASAPVA 95

Query: 88  EQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPRWRLLRKISTVHLFSSKALDDHR 147
            Q L+ H  NF +RPPN+GA+++AYN +D+VFAPYG RWR LRK+  +HLFS+KALDD R
Sbjct: 96  AQFLRGHDANFSNRPPNSGAEHVAYNYQDLVFAPYGARWRALRKLCALHLFSAKALDDLR 155

Query: 148 RIREEEIGVLVQALARSGEAPATLGSLLTVCTANVLGRTMISRRVFG-DGSGGDPEANIF 207
            +RE E+ ++V+ LAR   A   LG    VC  N L R  I  RVF  DG  G   A  F
Sbjct: 156 AVREGEVALMVRNLARQQAASVALGQEANVCATNTLARATIGHRVFAVDGGEG---AREF 215

Query: 208 KAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEMKKVHKWFDDFLTKIVEEHRNLLAGG 267
           K MV ++M L G FN GDF P+L WLD  GV ++MK++H+ +D+ +   + E +      
Sbjct: 216 KEMVVELMQLAGVFNVGDFVPALRWLDPQGVVAKMKRLHRRYDNMMNGFINERKAGAQPD 275

Query: 268 GDRGGD--QDLLSTLLSSKDE---AEDENEKLNDSEIKALLLNMFTAGTDTSASTVEWAL 327
           G   G+   DLLS LL+   E    + + EK+ +++IKALLLN+FTAGTDT++STVEWAL
Sbjct: 276 GVAAGEHGNDLLSVLLARMQEEQKLDGDGEKITETDIKALLLNLFTAGTDTTSSTVEWAL 335

Query: 328 TELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQAVVKETFRLHPPTPLSVPRMA 387
            ELIRHP ++ EAQ ELDTVVGR R VS+ DL +LPYL AV+KETFRLHP TPLS+PR A
Sbjct: 336 AELIRHPDVLKEAQHELDTVVGRGRLVSESDLPRLPYLTAVIKETFRLHPSTPLSLPREA 395

Query: 388 AESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPNRFLPGGEKAHVDVKGNDFEV 447
           AE CE++GY IPKG+TLLV++WAI+RDP +W DPL+++P+RFLPG   A VDVKG DF +
Sbjct: 396 AEECEVDGYRIPKGATLLVNVWAIARDPTQWPDPLQYQPSRFLPGRMHADVDVKGADFGL 455

Query: 448 IPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLLPDKLNMDEAYGLTLRRAQPL 507
           IPFGAGRRIC G+S GLRMV ++TATL H FDW L NG  PDKLNM+EAYGLTL+RA PL
Sbjct: 456 IPFGAGRRICAGLSWGLRMVTLMTATLVHGFDWTLANGATPDKLNMEEAYGLTLQRAVPL 515

Query: 508 VVHPRPRLLPGVY 515
           +V P PRLLP  Y
Sbjct: 516 MVQPVPRLLPSAY 524

BLAST of HG10008538 vs. ExPASy Swiss-Prot
Match: Q8LM92 (Flavonoid 3'-monooxygenase CYP75B4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B4 PE=2 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 1.6e-158
Identity = 285/522 (54.60%), Postives = 359/522 (68.77%), Query Frame = 0

Query: 6   LIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEY 65
           L   A+ V + YA++  RA   R    LPPGP+ WP++GN+PQ+GG   H  L  M   Y
Sbjct: 14  LTTVALSVIVCYALVFSRAGKARAPLPLPPGPRGWPVLGNLPQLGG-KTHQTLHEMTKVY 73

Query: 66  GPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPR 125
           GPL+ LR GS DVVVA SA VA Q L+ H  NF SRP N+G +++AYN RD+VF PYGPR
Sbjct: 74  GPLIRLRFGSSDVVVAGSAPVAAQFLRTHDANFSSRPRNSGGEHMAYNGRDVVFGPYGPR 133

Query: 126 WRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPAT-------LGSLLTVC 185
           WR +RKI  V+LFS++ALDD R  RE E  ++V++LA +  AP +       LG  + VC
Sbjct: 134 WRAMRKICAVNLFSARALDDLRAFREREAVLMVRSLAEASAAPGSSSPAAVVLGKEVNVC 193

Query: 186 TANVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVA 245
           T N L R  + RRVF  G+G    A  FK +V +VM + G  N GDF P+L WLD  GV 
Sbjct: 194 TTNALSRAAVGRRVFAAGAG--EGAREFKEIVLEVMEVGGVLNVGDFVPALRWLDPQGVV 253

Query: 246 SEMKKVHKWFDDFLTKIVEEHR--NLLAGGGDRGGDQDLLSTLLSSKDEAE----DENEK 305
           + MKK+H+ FDD +  I+ E R  +LL     R   +DLL  LL+   E E     E+++
Sbjct: 254 ARMKKLHRRFDDMMNAIIAERRAGSLLKPTDSREEGKDLLGLLLAMVQEQEWLAAGEDDR 313

Query: 306 LNDSEIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLD 365
           + D+EIKAL+LN+F AGTDT+++ VEW + ELIRHP I+  AQ+ELD VVGRDR +S+ D
Sbjct: 314 ITDTEIKALILNLFVAGTDTTSTIVEWTMAELIRHPDILKHAQEELDVVVGRDRLLSESD 373

Query: 366 LSQLPYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEW 425
           LS L +  A++KETFRLHP TPLS+PRMA+E CEI GY IPKG+ LLV++W I+RDP  W
Sbjct: 374 LSHLTFFHAIIKETFRLHPSTPLSLPRMASEECEIAGYRIPKGAELLVNVWGIARDPAIW 433

Query: 426 ADPLEFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSF 485
            DPLE++P+RFLPGG    VDVKGNDF +IPFGAGRRIC G+S GLRMV M  ATL H+F
Sbjct: 434 PDPLEYKPSRFLPGGTHTDVDVKGNDFGLIPFGAGRRICAGLSWGLRMVTMTAATLVHAF 493

Query: 486 DWALPNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
           DW LP    PDKLNMDEA+ L L+RA+PLVVHP PRLLP  Y
Sbjct: 494 DWQLPADQTPDKLNMDEAFTLLLQRAEPLVVHPVPRLLPSAY 532

BLAST of HG10008538 vs. ExPASy TrEMBL
Match: A0A5A7UTD5 (Flavonoid 3'-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002910 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.8e-259
Identity = 452/522 (86.59%), Postives = 479/522 (91.76%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+EI+LIVAA ++TLA A LLHR FFLRRRF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1   MEEITLIVAA-IITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKIS VHLFSSKALDDHRRIR++EI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 180

Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGDGSG D  E+N FKAMVEQVMVL GKFNPGDFFP LE LDL+GV S
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLA---GGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           EMKKVHKWFDDFLTKIVEEHRNLLA   GG   GG QDLLSTLLS KDE  DENEKLND+
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD+VVGRDRAVSDLDL QL
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPKEW DPL
Sbjct: 361 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
           EF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDWAL
Sbjct: 421 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 519
           PNGL PDKLNMDE YGLTLRRAQPLVVHPRPRLL GVYQKLP
Sbjct: 481 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of HG10008538 vs. ExPASy TrEMBL
Match: A0A1S3BVU8 (flavonoid 3'-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103494036 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 2.8e-259
Identity = 452/522 (86.59%), Postives = 479/522 (91.76%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+EI+LIVAA ++TLA A LLHR FFLRRRF LPPGPKPWPIVGNMPQIGGAPLHSLLAA
Sbjct: 1   MEEITLIVAA-IITLASAFLLHRTFFLRRRFRLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKIS VHLFSSKALDDHRRIR++EI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISAVHLFSSKALDDHRRIRQDEISVLVQALARSGEAPANLGSLLTVCIA 180

Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGDGSG D  E+N FKAMVEQVMVL GKFNPGDFFP LE LDL+GV S
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKAMVEQVMVLAGKFNPGDFFPWLERLDLMGVGS 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLA---GGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           EMKKVHKWFDDFLTKIVEEHRNLLA   GG   GG QDLLSTLLS KDE  DENEKLND+
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGGGGSGGGGHQDLLSTLLSMKDEGVDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWALTELIRHP++MA+AQ+ELD+VVGRDRAVSDLDL QL
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELDSVVGRDRAVSDLDLPQL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPKEW DPL
Sbjct: 361 VYLQAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKEWVDPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
           EF PNRFLP GEK HVDVKGNDFE IPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDWAL
Sbjct: 421 EFGPNRFLPDGEKPHVDVKGNDFEAIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQKLP 519
           PNGL PDKLNMDE YGLTLRRAQPLVVHPRPRLL GVYQKLP
Sbjct: 481 PNGLTPDKLNMDEHYGLTLRRAQPLVVHPRPRLLLGVYQKLP 521

BLAST of HG10008538 vs. ExPASy TrEMBL
Match: A0A6J1EZJ7 (flavonoid 3'-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111440987 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 8.5e-248
Identity = 432/519 (83.24%), Postives = 468/519 (90.17%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
           MDEISL  +A  +TLA  +LL+R  F +R      LPPGPKPWPIVGN+PQIGGAPLHSL
Sbjct: 1   MDEISLFFSA-AITLASVVLLYRLLFHQRNSRSLPLPPGPKPWPIVGNLPQIGGAPLHSL 60

Query: 61  LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
           LA M+GEYGPLMYLR GSVDVVVAASA++AEQ LKI  TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61  LADMSGEYGPLMYLRFGSVDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
           VFAPYGPRWRLLRKIS VHLFSSKAL+DHRR+REEE+GVLVQALARSGEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDHRRVREEEVGVLVQALARSGEAPATLGSLLTV 180

Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
           CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPGDFF  LEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGDFFSWLEWLDLLG 240

Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           VA EMKKVHKWFDD ++KI+EEHRNLLA GG  G  QDLLSTLLS++D A+DENEKLND+
Sbjct: 241 VAGEMKKVHKWFDDLMSKILEEHRNLLATGGG-GEHQDLLSTLLSTRDRAKDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD+VVGRDR VSDLDL +L
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMAAESCEI+GYHIPKGSTLLVD+WAISRDPKEW  PL
Sbjct: 361 GYLQAVVKETFRLHPPTPLSLPRMAAESCEISGYHIPKGSTLLVDVWAISRDPKEWTSPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
           EFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGLRMVQMLTA+L HSFDWAL
Sbjct: 421 EFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 516
           P+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 PSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 517

BLAST of HG10008538 vs. ExPASy TrEMBL
Match: A0A6J1L0D6 (flavonoid 3'-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111499893 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.6e-246
Identity = 429/519 (82.66%), Postives = 466/519 (89.79%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRR---FCLPPGPKPWPIVGNMPQIGGAPLHSL 60
           MDEISL  +A  +TLA  +LL+R  F +R      LPPGPKPWP VGN+PQIGGAPLHS 
Sbjct: 1   MDEISLFFSA-AITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQ 60

Query: 61  LAAMAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDM 120
           LAAM+GEYGPLMYLR GS+DVVVAASA++AEQ LKI  TNFLSRPPNAGAKYIAYN +DM
Sbjct: 61  LAAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDM 120

Query: 121 VFAPYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTV 180
           VFAPYGPRWRLLRKIS VHLFSSKAL+D+R +REEE+GVLVQALAR GEAPATLGSLLTV
Sbjct: 121 VFAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTV 180

Query: 181 CTANVLGRTMISRRVFGDGS-GGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLG 240
           CTAN LGRTMI RRVFGDGS GGDP ++ FKAMVEQVM+L GKFNPG+FFPSLEWLDLLG
Sbjct: 181 CTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLG 240

Query: 241 VASEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDS 300
           VA EMKKVHKWFDD L+KI+EEHR LLA GG+    QDLLSTLLS++D AEDENEKLND+
Sbjct: 241 VAREMKKVHKWFDDLLSKILEEHRTLLASGGEH---QDLLSTLLSTRDHAEDENEKLNDT 300

Query: 301 EIKALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQL 360
           EIKALLLNMFTAGTDTSASTVEWAL ELIRHPK+M +AQ+ELD+VVGRDR VSDLDL +L
Sbjct: 301 EIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKL 360

Query: 361 PYLQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPL 420
            YLQAVVKETFRLHPPTPLS+PRMAAESCEINGYHIPKGSTLLVD+WAISRDPKEW  PL
Sbjct: 361 GYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPL 420

Query: 421 EFRPNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWAL 480
           EFRP+RFLPGGEKAHVDVKGNDF VIPFGAGRRICVGMSMGLRMVQMLTA+L HSFDWAL
Sbjct: 421 EFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWAL 480

Query: 481 PNGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVYQ 516
           P+GL P +LNMDEA+GLTLRRAQPLV HPRPRL PGVYQ
Sbjct: 481 PSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ 515

BLAST of HG10008538 vs. ExPASy TrEMBL
Match: A0A0A0LP99 (Flavonoid 3' hydroxylase OS=Cucumis sativus OX=3659 GN=Csa_1G013190 PE=3 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.8e-227
Identity = 404/508 (79.53%), Postives = 434/508 (85.43%), Query Frame = 0

Query: 1   MDEISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAA 60
           M+EI+ IVAA ++TLA A LLHRAFFLRRR  LPPGPKPWPIVGNMPQIG A LHSLLA 
Sbjct: 1   MEEITPIVAA-LITLASAFLLHRAFFLRRRLRLPPGPKPWPIVGNMPQIGEAHLHSLLAT 60

Query: 61  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 120
           MAG+YGPLMYLRLGSVD+VVAASA+VAEQ+LKIH TNFLSRPPNAGAKYIAYN +DMVFA
Sbjct: 61  MAGKYGPLMYLRLGSVDMVVAASAAVAEQVLKIHDTNFLSRPPNAGAKYIAYNYQDMVFA 120

Query: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 180
           PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEI VLVQALARSGEAPA LGSLLTVC A
Sbjct: 121 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEISVLVQALARSGEAPANLGSLLTVCIA 180

Query: 181 NVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVAS 240
           N LGRTMI RRVFGDGSG D  E+N FK MVEQVMVL GKFNPGDFFP LEWLDL+GV  
Sbjct: 181 NSLGRTMIGRRVFGDGSGSDDLESNQFKLMVEQVMVLAGKFNPGDFFPWLEWLDLMGVGR 240

Query: 241 EMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIK 300
           EMKKVHKWFDDFLTKIVEEHRNLLA G   GG QDLLSTLLS KD+ EDENEKLND+EIK
Sbjct: 241 EMKKVHKWFDDFLTKIVEEHRNLLARGVG-GGHQDLLSTLLSMKDDGEDENEKLNDTEIK 300

Query: 301 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 360
           ALLL +F                           ++  L+  +GRDRAVSD+DL QL YL
Sbjct: 301 ALLLVLFL--------------------------SKFYLNCFIGRDRAVSDVDLHQLVYL 360

Query: 361 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 420
           QAVVKETFRLHPPTPLS+PRMA++SCE+NGYHIPKGSTLLVD+WAI RDPK+W DPLEFR
Sbjct: 361 QAVVKETFRLHPPTPLSLPRMASDSCEVNGYHIPKGSTLLVDVWAIGRDPKQWVDPLEFR 420

Query: 421 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNG 480
           PNRFLP GEK HVDVKGNDFEVIPFGAGRRICVG+S+GLRMVQMLTAT+ HSFDW LPNG
Sbjct: 421 PNRFLPNGEKPHVDVKGNDFEVIPFGAGRRICVGLSLGLRMVQMLTATIVHSFDWTLPNG 480

Query: 481 LLPDKLNMDEAYGLTLRRAQPLVVHPRP 508
           L PDKLNMDE YGLTLRRAQPL++HPRP
Sbjct: 481 LTPDKLNMDEHYGLTLRRAQPLIMHPRP 480

BLAST of HG10008538 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 643.7 bits (1659), Expect = 1.3e-184
Identity = 318/515 (61.75%), Postives = 396/515 (76.89%), Query Frame = 0

Query: 5   SLIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAA 64
           +L +  ++ T+ + IL  R F  RR       LPPGP PWPI+GN+P +G  P H  L+A
Sbjct: 3   TLFLTILLATVLFLIL--RIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSA 62

Query: 65  MAGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFA 124
           M   YGP+++LRLG VDVVVAAS SVAEQ LKIH  NF SRPPN+GAK++AYN +D+VFA
Sbjct: 63  MVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFA 122

Query: 125 PYGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEAPATLGSLLTVCTA 184
           PYG RWRLLRKIS+VHLFS+KAL+D + +R+EE+G L + L R G  P  LG L+ +C  
Sbjct: 123 PYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVV 182

Query: 185 NVLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASE 244
           N LGR MI RR+F  G+  D +A+ F++MV ++M L G FN GDF PSL+WLDL GVA +
Sbjct: 183 NALGREMIGRRLF--GADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGK 242

Query: 245 MKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSK-DEAEDENEKLNDSEIK 304
           MK++HK FD FL+ I++EH       G      D+LSTL+S K  + + +   L D+EIK
Sbjct: 243 MKRLHKRFDAFLSSILKEHEM----NGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIK 302

Query: 305 ALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYL 364
           ALLLNMFTAGTDTSASTV+WA+ ELIRHP IM +AQ+ELD VVGRDR V++ D++QLPYL
Sbjct: 303 ALLLNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYL 362

Query: 365 QAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFR 424
           QAV+KE FRLHPPTPLS+P +A+ESCEINGYHIPKGSTLL +IWAI+RDP +W+DPL F+
Sbjct: 363 QAVIKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFK 422

Query: 425 PNRFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNG 484
           P RFLPGGEK+ VDVKG+DFE+IPFGAGRRIC G+S+GLR +Q LTATL   FDW L  G
Sbjct: 423 PERFLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGG 482

Query: 485 LLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
           + P+KLNM+E+YGLTL+RA PLVVHP+PRL P VY
Sbjct: 483 VTPEKLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of HG10008538 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 340.9 bits (873), Expect = 1.8e-93
Identity = 183/503 (36.38%), Postives = 286/503 (56.86%), Query Frame = 0

Query: 9   AAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEYGPL 68
           A +++T  ++IL +  F    +  LPPGP+  PIVGN+P +    LH+    +A  +GP+
Sbjct: 21  AVLILTATFSILWY-IFKRSPQPPLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAQSHGPI 80

Query: 69  MYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYGPRWRL 128
             L LGS   VV  S S+A ++LK    NF +      A+ + Y   D+V+ PYG  WR+
Sbjct: 81  FKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRM 140

Query: 129 LRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSG--EAPATLGSLLTVCTANVLGRT 188
           LRK+    LFS K LD    +R +EI    + L + G  ++P  +G  L +   N++   
Sbjct: 141 LRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNM 200

Query: 189 MISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEMKKVHK 248
           +    V  +    +     FK ++ ++  L G  N  DFFP L   DL G+  +M    +
Sbjct: 201 LWGGSVKAEDM--ESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYAR 260

Query: 249 WFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIKALLLNMF 308
             D  L + +E+ + L +  GD G  +D L  L+  +D+  D +  +  + +KA+L++M 
Sbjct: 261 DLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHVKAVLMDMV 320

Query: 309 TAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQAVVKET 368
             GT++S +T+E+ + ELI +P++M  AQ+ELD VVG+D  V +  ++ LPY+ AV+KET
Sbjct: 321 VGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYILAVLKET 380

Query: 369 FRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPNRFLPG 428
            RL+P  PL VP   +E+  + GY IPK + + +++W+I RDP  W  P EFRP RFL  
Sbjct: 381 LRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPERFL-- 440

Query: 429 GEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLLPDKLN 488
            +K   D  G D+  +PFG+GRRIC G+++  RM+    ATL HSFDW +P+G +   L+
Sbjct: 441 -DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDGHV---LD 500

Query: 489 MDEAYGLTLRRAQPLVVHPRPRL 510
           ++E +G+ L+   PLV  P PRL
Sbjct: 501 LEEKFGIVLKLKTPLVALPIPRL 513

BLAST of HG10008538 vs. TAIR 10
Match: AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2 )

HSP 1 Score: 339.0 bits (868), Expect = 6.9e-93
Identity = 192/517 (37.14%), Postives = 285/517 (55.13%), Query Frame = 0

Query: 6   LIVAAVVVTLAYAILLHRAFFLRRRFC----LPPGPKPWPIVGNMPQIGGAPLHSLLAAM 65
           L++A++   L   +LL R  +L+   C    LPPGP   PI+GN+ Q+G  P H  LA++
Sbjct: 3   LVLASLFAVLILNVLLWR--WLKASACKAQRLPPGPPRLPILGNLLQLGPLP-HRDLASL 62

Query: 66  AGEYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAP 125
             +YGPL+YLRLG+VD +         ++L      F SRP    A ++AY   D+  AP
Sbjct: 63  CDKYGPLVYLRLGNVDAITTNDPDTIREILLRQDDVFSSRPKTLAAVHLAYGCGDVALAP 122

Query: 126 YGPRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSGEA--PATLGSLLTVCT 185
            GP W+ +R+I   HL ++K L+     R EE   L++ + +  E   P  L  +L   +
Sbjct: 123 MGPHWKRMRRICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVLGAFS 182

Query: 186 ANVLGRTMISRRVFGDGSGGDP-EANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVA 245
            N + R ++ ++ FG GS   P EA  F  +  ++  L G    GD+ P   W+D  G  
Sbjct: 183 MNNVTRMLLGKQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCE 242

Query: 246 SEMKKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEI 305
            EM+ V K  D+F TKI++EHR       D+ GD D +  LLS     E+    + D EI
Sbjct: 243 KEMRDVEKRVDEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSL--PGENGKAHMEDVEI 302

Query: 306 KALLLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPY 365
           KAL+ +M  A TDTSA T EWA+ E I+ P++M + Q+ELD VVG +R V + DL  L Y
Sbjct: 303 KALIQDMIAAATDTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNY 362

Query: 366 LQAVVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEF 425
           L+ VV+ETFR+HP  P  +P  +  +  INGY+IP  + + ++   + R+ K W D  +F
Sbjct: 363 LRCVVRETFRMHPAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDF 422

Query: 426 RPNRFLPGGEKAHVDVK-GNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALP 485
           RP R  P      V++  G DF+++PF AG+R C G  +G+ MV M  A L H F+W+ P
Sbjct: 423 RPERHWPVEGSGRVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWSSP 482

Query: 486 NGLLPDKLNMDEAYGLTLRRAQPLVVHPRPRLLPGVY 515
                  ++  E YG+T+ +A+PL    +PRL   +Y
Sbjct: 483 G-----NIDTVEVYGMTMPKAKPLRAIAKPRLAAHLY 509

BLAST of HG10008538 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 337.4 bits (864), Expect = 2.0e-92
Identity = 182/508 (35.83%), Postives = 285/508 (56.10%), Query Frame = 0

Query: 4   ISLIVAAVVVTLAYAILLHRAFFLRRRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAG 63
           I+L   A+++ +A   +L   F    +  LPPGP+  PIVGN+P +    LH+    +A 
Sbjct: 13  INLTPYAILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAE 72

Query: 64  EYGPLMYLRLGSVDVVVAASASVAEQLLKIHGTNFLSRPPNAGAKYIAYNNRDMVFAPYG 123
            YGP+  L LGS   VV  + S+A ++LK    NF +      A+ + Y   D+V+ PYG
Sbjct: 73  SYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYG 132

Query: 124 PRWRLLRKISTVHLFSSKALDDHRRIREEEIGVLVQALARSG--EAPATLGSLLTVCTAN 183
             WR+LRK+  + L S + L+    +R +EI    + L + G  E+P  +G  + +   N
Sbjct: 133 AEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMMN 192

Query: 184 VLGRTMISRRVFGDGSGGDPEANIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEM 243
           +    +    V  +    +     FK ++ ++  L G+ N  DFFP L   DL G+  +M
Sbjct: 193 LTMNMLWGGSVKAEEM--ESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKKM 252

Query: 244 KKVHKWFDDFLTKIVEEHRNLLAGGGDRGGDQDLLSTLLSSKDEAEDENEKLNDSEIKAL 303
               +  D  L + +E+ + L    GD G  +D L  L+  KD+  D    +  + +KA+
Sbjct: 253 HVCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVKAV 312

Query: 304 LLNMFTAGTDTSASTVEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQA 363
           L+++   GTDTS +T+E+A+ ELIR P++M  AQ+ELD VVG+D  + +  +++LP++ A
Sbjct: 313 LVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLPFISA 372

Query: 364 VVKETFRLHPPTPLSVPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPN 423
           ++KET RL+P  PL VP   +E+  + GY IPK + + +++W+I RDP  W  P EFRP 
Sbjct: 373 IMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPE 432

Query: 424 RFLPGGEKAHVDVKGNDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLL 483
           RFL   +K   D  G D+  +PFG+GRRIC G+++  RM+    ATL HSFDW +P G +
Sbjct: 433 RFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKIPEGHI 492

Query: 484 PDKLNMDEAYGLTLRRAQPLVVHPRPRL 510
              L++ E +G+ L+   PLV  P PRL
Sbjct: 493 ---LDLKEKFGIVLKLKSPLVALPVPRL 511

BLAST of HG10008538 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 333.6 bits (854), Expect = 2.9e-91
Identity = 190/489 (38.85%), Postives = 285/489 (58.28%), Query Frame = 0

Query: 29  RRFCLPPGPKPWPIVGNMPQIGGAPLHSLLAAMAGEYGPLMYLRLGSVDVVVAASASVAE 88
           R   LPPGP   PI+GN+ Q+G  P HS  A +A  YGP+M L+ G ++ VV  S   A 
Sbjct: 34  RAATLPPGPPRLPIIGNIHQVGKNP-HSSFADLAKIYGPIMSLKFGCLNSVVITSPEAAR 93

Query: 89  QLLKIHGTNFLSRPPNAGAKYIAYNNRDMVF-APYGPRWRLLRKISTVHLFSSKALDDHR 148
           ++L+ H      R  N   +   +    +++  P   RWR+LRK+S   +FS +  +  +
Sbjct: 94  EVLRTHDQILSGRKSNDSIRCFGHEEVSVIWLPPSSARWRMLRKLSVTLMFSPQRTEATK 153

Query: 149 RIREEEIGVLVQALARSGEAPATL----GSLLTVCTANVLGRTMISRRVFGDGSGGDPEA 208
            +R +++  LV  +  S E    +     S  TV   N++   + S  +   GS    ++
Sbjct: 154 ALRMKKVQELVSFMNESSERKEAVDISRASYTTV--LNIISNILFSVDL---GSYDSKKS 213

Query: 209 NIFKAMVEQVMVLPGKFNPGDFFPSLEWLDLLGVASEMK----KVHKWFDDFL-TKIVEE 268
           N F+  V   M   GK +  ++FP + +LDL G    M+    ++ + F  F+  KI E+
Sbjct: 214 NEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGFMDAKIAEK 273

Query: 269 HRNLLAGGGDRGGDQDLLSTL--LSSKDEAEDENEKLNDSEIKALLLNMFTAGTDTSAST 328
               L        ++D L +L  L+  DEAE     L++++I+ LLL+MFTAGTDTS+ST
Sbjct: 274 S---LGNYSKDVSNRDFLDSLLILNEGDEAE-----LDNNDIEHLLLDMFTAGTDTSSST 333

Query: 329 VEWALTELIRHPKIMAEAQKELDTVVGRDRAVSDLDLSQLPYLQAVVKETFRLHPPTPLS 388
           +EWA+ EL+R+PK M +AQ E+D V+G++  V + D+S LPYLQAVVKETFRLHP  PL 
Sbjct: 334 LEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPAAPLL 393

Query: 389 VPRMAAESCEINGYHIPKGSTLLVDIWAISRDPKEWADPLEFRPNRFLPGGEKAHVDVKG 448
           VPR A    E+ G+ +PK + +LV++WAI RDP  W +P +F P RF+       +DVKG
Sbjct: 394 VPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFM----GKDIDVKG 453

Query: 449 NDFEVIPFGAGRRICVGMSMGLRMVQMLTATLAHSFDWALPNGLLPDKLNMDEAYGLTLR 506
            D+E+ PFG GRRIC G+ + ++ V ++ A+L +SFDW LPNG++ + L+MDE +G+TL 
Sbjct: 454 RDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLH 504