HG10023033 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10023033 vs. NCBI nr
Match: NP_001267675.1 (steryl-sulfatase [Cucumis sativus] >ABQ53598.1 stachyose synthase [Cucumis sativus])

HSP 1 Score: 1755.3 bits (4545), Expect = 0.0e+00
Identity = 833/864 (96.41%), Postives = 852/864 (98.61%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKS+GLENLIDFS+GKISVKGVP+LSEVPTNVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPS+RLTN LGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFD 180
           SDLQMETQWVMLN+PEIKSYVVIIPIIEGSFRSA+HPGTDGQVLICAESGS HVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVG 240
           AIAY+HVSDNPY+LMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGV+DFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAKAIEIEHAEK+RDKAIGSGVT+VSKFET IQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           K+EL  IFGKEEEEESSAI+KGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THL+SKI+PCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720
           NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIK 780
           DQKPEAAPMGNFVEYIVYLNQA QILHTTPKSEPLKATI PSTFELFNFIPLRKLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDS 840
           FAPIGLTNMFN SGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKC+SNGIEVEFEW S
Sbjct: 781 FAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIEVEFEWKS 840

Query: 841 DGKLSFDLHWIEEAGGVSNLDILF 865
           DGKLSFDLHWIEEAGGVSNLDI F
Sbjct: 841 DGKLSFDLHWIEEAGGVSNLDIFF 864

BLAST of HG10023033 vs. NCBI nr
Match: KAA0046706.1 (stachyose synthase [Cucumis melo var. makuwa] >TYK18242.1 stachyose synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1753.4 bits (4540), Expect = 0.0e+00
Identity = 830/864 (96.06%), Postives = 853/864 (98.73%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKS+GLENLIDFS+GKISVKGVP+LSEVP NVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPS+RLTN LGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFD 180
           SDLQMETQWVMLN+PEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGS HVKASSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVG 240
           AIAY+HVSDNPY+LMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAKAIEIEHAEK+RDKAIGSGVT+VSKFET IQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           K+ELL+IFG EEEEES+AI+KGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELLEIFGNEEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THL+SKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720
           NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIK 780
           DQKPEAAPMGNFVEYIVYLNQA QI+HTTPKSEPLKATI PSTFELFNFIPLRKLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQIIHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDS 840
           FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNG E++F W+S
Sbjct: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGTELKFVWNS 840

Query: 841 DGKLSFDLHWIEEAGGVSNLDILF 865
           DGKLSFD+HWIEEAGG+SNLDI F
Sbjct: 841 DGKLSFDVHWIEEAGGISNLDIFF 864

BLAST of HG10023033 vs. NCBI nr
Match: ABV44498.1 (stachyose synthetase variant 1 [Cucumis sativus] >ABV44499.1 stachyose synthetase variant 2 [Cucumis sativus] >ABV44500.1 stachyose synthetase variant 3 [Cucumis sativus])

HSP 1 Score: 1753.0 bits (4539), Expect = 0.0e+00
Identity = 832/864 (96.30%), Postives = 851/864 (98.50%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKS+GLENLIDFS+GKISVKGVP+LSEVPTNVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPS+RLTN LGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFD 180
           SDLQMETQWVMLN+PEIKSYVVIIPIIEGSFRSA+HPGTDGQVLICAESGS HVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVG 240
           AIAY+HVSDNPY+LMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGV+DFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAKAIEIEHAEK+RDKAIGSGVT+VSKFET IQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           K+EL  IFGKEEEEESSAI+KGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THL+SKI+PCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720
           NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIK 780
           DQKPEAAPMGNFVEYIVYLNQA QILHTTPKSEPLKATI PSTFELFNFIPLRKLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDS 840
           FAPIGLTNMFN SGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKC+SNGIEVEFEW S
Sbjct: 781 FAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIEVEFEWKS 840

Query: 841 DGKLSFDLHWIEEAGGVSNLDILF 865
           DGKLSFDLHWIEEAGGV NLDI F
Sbjct: 841 DGKLSFDLHWIEEAGGVFNLDIFF 864

BLAST of HG10023033 vs. NCBI nr
Match: XP_008451468.1 (PREDICTED: stachyose synthase [Cucumis melo])

HSP 1 Score: 1752.3 bits (4537), Expect = 0.0e+00
Identity = 829/864 (95.95%), Postives = 853/864 (98.73%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKS+GLENLIDFS+GKISVKGVP+LSEVP NVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPS+RLTN LGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFD 180
           SDLQMETQWVMLN+PEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGS HVKASSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVG 240
           AIAY+HVSDNPY+LMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLY FDECEK
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYSFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKL 360
           FRKYKGGSL+GPNAPSFDPKKPKLLIAKAIEIEHAEK+RDKAIGSGVT+VSKFET IQKL
Sbjct: 301 FRKYKGGSLMGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           K+ELL+IFG EEEEES+AI+KGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELLEIFGNEEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THL+SKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720
           NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIK 780
           DQKPEAAPMGNFVEYIVYLNQA QI+HTTPKSEPLKATI PSTFELFNFIPLRKLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQIIHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDS 840
           FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNG E++F W+S
Sbjct: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGTELKFVWNS 840

Query: 841 DGKLSFDLHWIEEAGGVSNLDILF 865
           DGKLSFD+HWIEEAGG+SNLDI F
Sbjct: 841 DGKLSFDVHWIEEAGGISNLDIFF 864

BLAST of HG10023033 vs. NCBI nr
Match: XP_038897999.1 (stachyose synthase [Benincasa hispida])

HSP 1 Score: 1747.6 bits (4525), Expect = 0.0e+00
Identity = 830/864 (96.06%), Postives = 852/864 (98.61%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKSE LENLIDF +GKISVKGVP+LSEVPTNVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSESLENLIDFLDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGF++TQPS+RLTN LGKFKGREFVS+FRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFNRTQPSDRLTNSLGKFKGREFVSIFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFD 180
           SDLQMETQWVMLN+PEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGS HVKASSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVG 240
           AIAY+HVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKL 360
           FRKYKGGSL GP+APSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTD+SKFET I+KL
Sbjct: 301 FRKYKGGSLSGPSAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDISKFETKIKKL 360

Query: 361 KKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           K+EL +IFGKEEEE+S+AISKGCTSCSCKA+NSGMKAFTRDLRT+FKGLDDIFVWHALAG
Sbjct: 361 KEELHEIFGKEEEEDSTAISKGCTSCSCKAENSGMKAFTRDLRTQFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPGSTHL+SKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGSTHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720
           NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKG+PECYKPMSTTVHVNDIEW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGYPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIK 780
           DQKPEAAPMGNFVEYIVYLNQA QILHTTPKSEPLKATI PSTFELFNFIPLRKLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDS 840
           FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGG+FLAYSSGSPKKCLSNGIEVEFEWDS
Sbjct: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGDFLAYSSGSPKKCLSNGIEVEFEWDS 840

Query: 841 DGKLSFDLHWIEEAGGVSNLDILF 865
           DGKLSFDL WIEE GGVSNLDI F
Sbjct: 841 DGKLSFDLPWIEETGGVSNLDIFF 864

BLAST of HG10023033 vs. ExPASy Swiss-Prot
Match: Q93XK2 (Stachyose synthase OS=Pisum sativum OX=3888 GN=STS1 PE=1 SV=1)

HSP 1 Score: 1209.9 bits (3129), Expect = 0.0e+00
Identity = 567/866 (65.47%), Postives = 688/866 (79.45%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQ--SS 60
           MAPP     LN++       E++ D SE K  VKG PL  +VP NV F  FSSI +   S
Sbjct: 1   MAPP-----LNSTTSNLIKTESIFDLSERKFKVKGFPLFHDVPENVSFRSFSSICKPSES 60

Query: 61  DAPLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGN 120
           +AP  LLQ+V + S+KGGF GF    PS+RL N +G F G++F+S+FRFKTWWST W+G 
Sbjct: 61  NAPPSLLQKVLAYSHKGGFFGFSHETPSDRLMNSIGSFNGKDFLSIFRFKTWWSTQWIGK 120

Query: 121 SGSDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASS 180
           SGSDLQMETQW+++ VPE KSYVVIIPIIE  FRSAL PG +  V I AESGS  VK S+
Sbjct: 121 SGSDLQMETQWILIEVPETKSYVVIIPIIEKCFRSALFPGFNDHVKIIAESGSTKVKEST 180

Query: 181 FDAIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEP 240
           F++IAY+H S+NPY LMKEAY+A+RVHLN+FRLLEEK + +LVDKFGWCTWDAFYLTV P
Sbjct: 181 FNSIAYVHFSENPYDLMKEAYSAIRVHLNSFRLLEEKTIPNLVDKFGWCTWDAFYLTVNP 240

Query: 241 VGIWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDEC 300
           +GI++G++DF +GG+ PRF+IIDDGWQSI+ DG DP  DAKNLVLGG QM+ RL+RFDEC
Sbjct: 241 IGIFHGLDDFSKGGVEPRFVIIDDGWQSISFDGYDPNEDAKNLVLGGEQMSGRLHRFDEC 300

Query: 301 EKFRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQ 360
            KFRKY+ G LLGPN+P +DP     LI K IE E   K R++AI S  +D+++ E+ I+
Sbjct: 301 YKFRKYESGLLLGPNSPPYDPNNFTDLILKGIEHEKLRKKREEAISSKSSDLAEIESKIK 360

Query: 361 KLKKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHAL 420
           K+ KE+  +FG E+       S G    S      G+KAFT+DLRTKFKGLDD++VWHAL
Sbjct: 361 KVVKEIDDLFGGEQ------FSSG--EKSEMKSEYGLKAFTKDLRTKFKGLDDVYVWHAL 420

Query: 421 AGAWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMH 480
            GAWGGVRP +THL +KI+PCKLSPGLDGTM DLAVV+I + S+GLVHP QA++ +DSMH
Sbjct: 421 CGAWGGVRPETTHLDTKIVPCKLSPGLDGTMEDLAVVEISKASLGLVHPSQANELYDSMH 480

Query: 481 SYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQC 540
           SYL++ GITGVKVDV+H+LEYV +EYGGRVDLAK YY+GLT S+VKNF G G+ +SMQ C
Sbjct: 481 SYLAESGITGVKVDVIHSLEYVCDEYGGRVDLAKVYYEGLTKSIVKNFNGNGMIASMQHC 540

Query: 541 NDFFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDM 600
           NDFF+LGTKQ S+GRVGDDFWFQDPNGDPMG +WLQGVHMIHC+YNS+WMGQ+IQPDWDM
Sbjct: 541 NDFFFLGTKQISMGRVGDDFWFQDPNGDPMGSFWLQGVHMIHCSYNSLWMGQMIQPDWDM 600

Query: 601 FQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCL 660
           FQSDH+CAKFHAGSRAICGGP+YVSD+VG H+FDLIK+LV+PDGTIP+C +F LPTRDCL
Sbjct: 601 FQSDHVCAKFHAGSRAICGGPIYVSDNVGSHDFDLIKKLVFPDGTIPKCIYFPLPTRDCL 660

Query: 661 FKNPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDI 720
           FKNPLFD+ TVLKIWN NKYGGVIG FNCQGAGWDP  Q+ +G PECYKP+  TVHV ++
Sbjct: 661 FKNPLFDHTTVLKIWNFNKYGGVIGAFNCQGAGWDPIMQKFRGFPECYKPIPGTVHVTEV 720

Query: 721 EWDQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSN 780
           EWDQK E + +G   EY+VYLNQA ++   T KSEP++ TI PSTFEL++F+P+ KL   
Sbjct: 721 EWDQKEETSHLGKAEEYVVYLNQAEELSLMTLKSEPIQFTIQPSTFELYSFVPVTKLCGG 780

Query: 781 IKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEW 840
           IKFAPIGLTNMFNS GT+  L+Y  NG ++KVKGGG+FLAYSS SPKK   NG EV+FEW
Sbjct: 781 IKFAPIGLTNMFNSGGTVIDLEYVGNGAKIKVKGGGSFLAYSSESPKKFQLNGCEVDFEW 840

Query: 841 DSDGKLSFDLHWIEEAGGVSNLDILF 865
             DGKL  ++ WIEEA GVS+++I F
Sbjct: 841 LGDGKLCVNVPWIEEACGVSDMEIFF 853

BLAST of HG10023033 vs. ExPASy Swiss-Prot
Match: Q9SYJ4 (Probable galactinol--sucrose galactosyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=RFS4 PE=2 SV=3)

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 519/855 (60.70%), Postives = 650/855 (76.02%), Query Frame = 0

Query: 22  NLIDFSEGKISVK-GVPLLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGF 81
           N  + SEG +  K   P+L +VP NV F+PFSS S S+DAPLP+L RV + ++KGGFLGF
Sbjct: 32  NSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGF 91

Query: 82  DQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVMLNVPEIKSY 141
            +  PS+RLTN LG+F+ REF+S+FRFK WWST W+G SGSDLQ ETQWVML +PEI SY
Sbjct: 92  TKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIPEIDSY 151

Query: 142 VVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFDAIAYIHVSDNPYKLMKEAYA 201
           V IIP IEG+FR++L PG  G VLICAESGS  VK SSF +IAYIH+ DNPY LMKEA++
Sbjct: 152 VAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEAFS 211

Query: 202 AVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVGIWNGVNDFVEGGISPRFLII 261
           A+RVH+NTF+LLEEK +  +VDKFGWCTWDA YLTV+P  IW GV +F +GG+ P+F+II
Sbjct: 212 ALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFVII 271

Query: 262 DDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLLGPNAPSFDPK 321
           DDGWQSIN DG++  +DA+NLVLGG QMTARL  F EC+KFR YKGGS +  +A  F+P 
Sbjct: 272 DDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPL 331

Query: 322 KPKLLIAKAIEIEHAEKDRDKAI-GSGVTDVSKFETTIQKLKKELLQIFGKEEEEESSAI 381
           KPK+LI KA E   A   R K +  SG  D+++ +  I+ L +EL  +F + E+EES   
Sbjct: 332 KPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLG- 391

Query: 382 SKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGS-THLSSKIIP 441
                  S     SGM AFT+DLR +FK LDDI+VWHAL GAW GVRP +   L +K+ P
Sbjct: 392 -------SDDVSGSGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKVAP 451

Query: 442 CKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMHTLE 501
            +LSP L  TM DLAV K++E  IGLVHP +A +F+DSMHSYL+ VG+TG K+DV  TLE
Sbjct: 452 FELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDVFQTLE 511

Query: 502 YVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYLGTKQNSIGRVGDDF 561
            ++EE+GGRV+LAKAYY GLT S++KNF GT + +SMQQCN+FF+L TKQ SIGRVGDDF
Sbjct: 512 SLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQISIGRVGDDF 571

Query: 562 WFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGG 621
           W+QDP GDP GVYWLQGVHMIHC+YNS+WMGQ+IQPDWDMFQSDH+CA++HA SRAICGG
Sbjct: 572 WWQDPYGDPQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAICGG 631

Query: 622 PVYVSDSVG--GHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLN 681
           PVY+SD +G   HNFDLIK+L + DGTIPRC H+ALPTRD LFKNPLFD +++LKI+N N
Sbjct: 632 PVYLSDHLGKASHNFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKESILKIFNFN 691

Query: 682 KYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEWDQKPEAAPMGNFV--- 741
           K+GGVIGTFNCQGAGW P+E R KG+ ECY  +S TVHV+DIEWDQ PEAA  G+ V   
Sbjct: 692 KFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAA--GSQVTYT 751

Query: 742 -EYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKL-GSNIKFAPIGLTNMFN 801
            +Y+VY  Q+ +IL    KSE +K T+ PS F+L +F+P+ +L  S ++FAP+GL NMFN
Sbjct: 752 GDYLVYKQQSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFAPLGLINMFN 811

Query: 802 SSGTIQHLKY-NENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDSD-GKLSFDLH 861
             GT+Q +K   +N + + VKG G F+AYSS +P KC  N  E EF+W+ + GKLSF + 
Sbjct: 812 CVGTVQDMKVTGDNSIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWEEETGKLSFFVP 871

Query: 862 WIEEAGGVSNLDILF 865
           W+EE+GG+S+L   F
Sbjct: 872 WVEESGGISHLSFTF 876

BLAST of HG10023033 vs. ExPASy Swiss-Prot
Match: Q9FND9 (Probable galactinol--sucrose galactosyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=RFS5 PE=1 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 6.6e-210
Identity = 375/867 (43.25%), Postives = 513/867 (59.17%), Query Frame = 0

Query: 16  KSEGLENLIDFSE------GKISVKGVPLLSEVPTNVFFSPFSSISQSSDAPLPLLQRVH 75
           KS+   N +DF+E        +   G  +L++VP NV  +    +      PL +     
Sbjct: 8   KSDSGINGVDFTEKFRLEDSTLLANGQVVLTDVPVNVTLTSSPYLVDKDGVPLDV----- 67

Query: 76  SLSYKGGFLGFD-QTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQ 135
                G F+GF+   +P       +GK K   F+S+FRFK WW+T WVG++G D++ ETQ
Sbjct: 68  ---SAGSFIGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQ 127

Query: 136 WVMLNVPEIKS---------YVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSF 195
            ++L+     S         YV+++P++EGSFRS+   G D  V +C ESGS  V  S F
Sbjct: 128 IIILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEF 187

Query: 196 DAIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPV 255
             I Y+H  D+P+KL+K+A   +RVH+NTF+LLEEK    +VDKFGWCTWDAFYLTV P 
Sbjct: 188 RQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPD 247

Query: 256 GIWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECE 315
           G+  GV   V+GG  P  ++IDDGWQSI  D +    +  N+ + G QM  RL +F+E  
Sbjct: 248 GVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENH 307

Query: 316 KFRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQK 375
           KF+ Y                              + KD+                    
Sbjct: 308 KFKDYV-----------------------------SPKDQ-------------------- 367

Query: 376 LKKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALA 435
                                          ++ GMKAF RDL+ +F  +D I+VWHAL 
Sbjct: 368 -------------------------------NDVGMKAFVRDLKDEFSTVDYIYVWHALC 427

Query: 436 GAWGGVRPGSTHL-SSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMH 495
           G WGG+RP +  L  S II  +LSPGL  TM DLAV KIIE  IG   PD A +F++ +H
Sbjct: 428 GYWGGLRPEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLH 487

Query: 496 SYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQC 555
           S+L   GI GVKVDV+H LE + ++YGGRVDLAKAY+K LT+S+ K+F G G+ +SM+ C
Sbjct: 488 SHLQNAGIDGVKVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHC 547

Query: 556 NDFFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDM 615
           NDF +LGT+  S+GRVGDDFW  DP+GDP G +WLQG HM+HCAYNS+WMG  IQPDWDM
Sbjct: 548 NDFMFLGTEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDM 607

Query: 616 FQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCL 675
           FQS H CA+FHA SRAI GGP+Y+SD VG H+FDL+K+LV P+G+I RC+++ALPTRD L
Sbjct: 608 FQSTHPCAEFHAASRAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRL 667

Query: 676 FKNPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDI 735
           F++PL D KT+LKIWNLNKY GVIG FNCQG GW  + +R +   EC   ++ T    D+
Sbjct: 668 FEDPLHDGKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDV 727

Query: 736 EWDQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKL-GS 795
           EW+       + N  E+ ++L+Q+ ++L  +  ++ L+ T+ P  FEL    P+  + G+
Sbjct: 728 EWNSGSSPISIANVEEFALFLSQSKKLL-LSGLNDDLELTLEPFKFELITVSPVVTIEGN 783

Query: 796 NIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFE 855
           +++FAPIGL NM N+SG I+ L YN+  VE+ V G G F  Y+S  P  CL +G  VEF 
Sbjct: 788 SVRFAPIGLVNMLNTSGAIRSLVYNDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFG 783

Query: 856 WDSDGKLSFDLHWIEEAGGVSNLDILF 865
           ++ D  +   + W     G+S++  LF
Sbjct: 848 YE-DSMVMVQVPW-SGPDGLSSIQYLF 783

BLAST of HG10023033 vs. ExPASy Swiss-Prot
Match: Q8VWN6 (Galactinol--sucrose galactosyltransferase OS=Pisum sativum OX=3888 GN=RFS PE=1 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 7.2e-209
Identity = 374/859 (43.54%), Postives = 503/859 (58.56%), Query Frame = 0

Query: 1   MAPPN-DPAALNASVLKSEGLEN--LIDFS---EGKISVKGVPLLSEVPTNVFF------ 60
           MAPP+    A    V+ +  + N  L+  S        V G P L++VP N+        
Sbjct: 1   MAPPSITKTATQQDVISTVDIGNSPLLSISLDQSRNFLVNGHPFLTQVPPNITTTTTSTP 60

Query: 61  SPFSSISQSSDAPLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFK 120
           SPF     + D    +    ++L  +G F+GF+ T+       PLGK KG +F S+FRFK
Sbjct: 61  SPFLDFKSNKDT---IANNNNTLQQQGCFVGFNTTEAKSHHVVPLGKLKGIKFTSIFRFK 120

Query: 121 TWWSTMWVGNSGSDLQMETQWVML--NVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLIC 180
            WW+T WVG +G +LQ ETQ ++L  N+   + YV+++PI+E SFR++L PG +  V + 
Sbjct: 121 VWWTTHWVGTNGHELQHETQILILDKNISLGRPYVLLLPILENSFRTSLQPGLNDYVDMS 180

Query: 181 AESGSAHVKASSFDAIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGW 240
            ESGS HV  S+F A  Y+H+S++PY+L+KEA   ++  L TF+ LEEK    +++KFGW
Sbjct: 181 VESGSTHVTGSTFKACLYLHLSNDPYRLVKEAVKVIQTKLGTFKTLEEKTPPSIIEKFGW 240

Query: 241 CTWDAFYLTVEPVGIWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPT--RDAKNLVLG 300
           CTWDAFYL V P G+W GV    +GG  P F+IIDDGWQSI+ D +DP   RD  N    
Sbjct: 241 CTWDAFYLKVHPKGVWEGVKALTDGGCPPGFVIIDDGWQSISHDDDDPVTERDGMNRTSA 300

Query: 301 GTQMTARLYRFDECEKFRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIG 360
           G QM  RL +++E  KFR+Y+ G                                     
Sbjct: 301 GEQMPCRLIKYEENYKFREYENGD------------------------------------ 360

Query: 361 SGVTDVSKFETTIQKLKKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRT 420
                                                            G+  F RDL+ 
Sbjct: 361 --------------------------------------------NGGKKGLVGFVRDLKE 420

Query: 421 KFKGLDDIFVWHALAGAWGGVRPGSTHL-SSKIIPCKLSPGLDGTMTDLAVVKIIEGSIG 480
           +F+ ++ ++VWHAL G WGGVRP    +  +K++  KLSPG+  TM DLAV KI+E  +G
Sbjct: 421 EFRSVESVYVWHALCGYWGGVRPKVCGMPEAKVVVPKLSPGVKMTMEDLAVDKIVENGVG 480

Query: 481 LVHPDQADDFFDSMHSYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLV 540
           LV P+ A + FD +HS+L   GI GVKVDV+H LE +SEEYGGRV+LAKAYYK LT+S+ 
Sbjct: 481 LVPPNLAQEMFDGIHSHLESAGIDGVKVDVIHLLELLSEEYGGRVELAKAYYKALTSSVN 540

Query: 541 KNFKGTGLFSSMQQCNDFFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAY 600
           K+FKG G+ +SM+ CNDFF LGT+  S+GRVGDDFW  DP+GDP G YWLQG HM+HCAY
Sbjct: 541 KHFKGNGVIASMEHCNDFFLLGTEAISLGRVGDDFWCCDPSGDPNGTYWLQGCHMVHCAY 600

Query: 601 NSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGT 660
           NS+WMG  I PDWDMFQS H CA+FHA SRAI GGPVYVSD VG HNF L+K  V PDG+
Sbjct: 601 NSLWMGNFIHPDWDMFQSTHPCAEFHAASRAISGGPVYVSDCVGNHNFKLLKSFVLPDGS 660

Query: 661 IPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHP 720
           I RCQH+ALPTRDCLF++PL + KT+LKIWNLNKY GV+G FNCQG GW P+ +R K   
Sbjct: 661 ILRCQHYALPTRDCLFEDPLHNGKTMLKIWNLNKYAGVLGLFNCQGGGWCPETRRNKSAS 720

Query: 721 ECYKPMSTTVHVNDIEWDQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPST 780
           E    ++      DIEW        +     + VY  +  + L     S+ L+ ++ P +
Sbjct: 721 EFSHAVTCYASPEDIEWCNGKTPMDIKGVDVFAVYFFKEKK-LSLMKCSDRLEVSLEPFS 775

Query: 781 FELFNFIPLRKLGSN-IKFAPIGLTNMFNSSGTIQHLKYNENG--VELKVKGGGNFLAYS 840
           FEL    PL+      I+FAPIGL NM NS G +Q L+++++   V++ V+G G    ++
Sbjct: 781 FELMTVSPLKVFSKRLIQFAPIGLVNMLNSGGAVQSLEFDDSASLVKIGVRGCGELSVFA 775

BLAST of HG10023033 vs. ExPASy Swiss-Prot
Match: Q5VQG4 (Galactinol--sucrose galactosyltransferase OS=Oryza sativa subsp. japonica OX=39947 GN=RFS PE=1 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 2.3e-199
Identity = 358/852 (42.02%), Postives = 487/852 (57.16%), Query Frame = 0

Query: 31  ISVKGVPLLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGFDQTQPSERLT 90
           ++V G P L +VP N+  +P S++  +SD P          +  G FLGFD     +R  
Sbjct: 35  LAVDGHPFLLDVPANIRLTPASTLVPNSDVP---------AAAAGSFLGFDAPAAKDRHV 94

Query: 91  NPLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVMLNVPEIKS-------YVVI 150
            P+GK +   F+S+FRFK WW+T WVG +G D++ ETQ ++L+    KS       YV++
Sbjct: 95  VPIGKLRDTRFMSIFRFKVWWTTHWVGTNGRDVENETQMMILDQSGTKSSPTGPRPYVLL 154

Query: 151 IPIIEGSFRSALHPG-TDGQVLICAESGSAHVKASSFDAIAYIHVSDNPYKLMKEAYAAV 210
           +PI+EG FR+ L  G  +  V +  ESGS+ V+ S F +  Y+H  D+P+ L+K+A   V
Sbjct: 155 LPIVEGPFRACLESGKAEDYVHMVLESGSSTVRGSVFRSAVYLHAGDDPFDLVKDAMRVV 214

Query: 211 RVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVGIWNGVNDFVEGGISPRFLIIDD 270
           R HL TFRL+EEK    +VDKFGWCTWDAFYL V P G+W GV    +GG  P  ++IDD
Sbjct: 215 RAHLGTFRLMEEKTPPPIVDKFGWCTWDAFYLKVHPEGVWEGVRRLADGGCPPGLVLIDD 274

Query: 271 GWQSINLDGEDPTRDAK--NLVLGGTQMTARLYRFDECEKFRKYKGGSLLGPNAPSFDPK 330
           GWQSI  D +D    A+  N    G QM  RL +F E  KFR+YKG              
Sbjct: 275 GWQSICHDDDDLGSGAEGMNRTSAGEQMPCRLIKFQENYKFREYKG-------------- 334

Query: 331 KPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKLKKELLQIFGKEEEEESSAIS 390
                                                                       
Sbjct: 335 ------------------------------------------------------------ 394

Query: 391 KGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGSTHL-SSKIIPC 450
                        GM  F R+++  F  ++ ++VWHAL G WGG+RPG+  L  +K++  
Sbjct: 395 -------------GMGGFVREMKAAFPTVEQVYVWHALCGYWGGLRPGAPGLPPAKVVAP 454

Query: 451 KLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMHTLEY 510
           +LSPGL  TM DLAV KI+   +GLV P +A + ++ +HS+L   GI GVKVDV+H LE 
Sbjct: 455 RLSPGLQRTMEDLAVDKIVNNGVGLVDPRRARELYEGLHSHLQASGIDGVKVDVIHLLEM 514

Query: 511 VSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYLGTKQNSIGRVGDDFW 570
           V EEYGGRV+LAKAY+ GLT S+ ++F G G+ +SM+ CNDF  LGT+  ++GRVGDDFW
Sbjct: 515 VCEEYGGRVELAKAYFAGLTESVRRHFNGNGVIASMEHCNDFMLLGTEAVALGRVGDDFW 574

Query: 571 FQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGGP 630
             DP+GDP G +WLQG HM+HCAYNS+WMG  I PDWDMFQS H CA FHA SRA+ GGP
Sbjct: 575 CTDPSGDPDGTFWLQGCHMVHCAYNSLWMGAFIHPDWDMFQSTHPCAAFHAASRAVSGGP 634

Query: 631 VYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLNKYG 690
           VYVSD+VG H+FDL+++L  PDGTI RC+ +ALPTRDCLF +PL D KT+LKIWN+NK+ 
Sbjct: 635 VYVSDAVGCHDFDLLRRLALPDGTILRCERYALPTRDCLFADPLHDGKTMLKIWNVNKFS 694

Query: 691 GVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEWDQKPEAAPMGNFVEYIVYL 750
           GV+G FNCQG GW  + +R         P++      D+EW         G    + VY 
Sbjct: 695 GVLGAFNCQGGGWSREARRNMCAAGFSVPVTARASPADVEWSHGG-----GGGDRFAVYF 754

Query: 751 NQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGS---NIKFAPIGLTNMFNSSGTI 810
            +A + L    + E ++ T+ P T+EL    P+R + S    I FAPIGL NM N+ G +
Sbjct: 755 VEARK-LQLLRRDESVELTLEPFTYELLVVAPVRAIVSPELGIGFAPIGLANMLNAGGAV 783

Query: 811 QHL----KYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDSDGKLSFDLHWIE 865
           Q      K  +   E+ VKG G  +AYSS  P+ C  NG + EF+++ DG ++ D+ W  
Sbjct: 815 QGFEAARKDGDVAAEVAVKGAGEMVAYSSARPRLCKVNGQDAEFKYE-DGIVTVDVPWTG 783

BLAST of HG10023033 vs. ExPASy TrEMBL
Match: B8LG99 (Stachyose synthase OS=Cucumis sativus OX=3659 GN=Csa_7G407800 PE=2 SV=1)

HSP 1 Score: 1755.3 bits (4545), Expect = 0.0e+00
Identity = 833/864 (96.41%), Postives = 852/864 (98.61%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKS+GLENLIDFS+GKISVKGVP+LSEVPTNVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPS+RLTN LGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFD 180
           SDLQMETQWVMLN+PEIKSYVVIIPIIEGSFRSA+HPGTDGQVLICAESGS HVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVG 240
           AIAY+HVSDNPY+LMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGV+DFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAKAIEIEHAEK+RDKAIGSGVT+VSKFET IQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           K+EL  IFGKEEEEESSAI+KGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THL+SKI+PCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720
           NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIK 780
           DQKPEAAPMGNFVEYIVYLNQA QILHTTPKSEPLKATI PSTFELFNFIPLRKLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDS 840
           FAPIGLTNMFN SGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKC+SNGIEVEFEW S
Sbjct: 781 FAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIEVEFEWKS 840

Query: 841 DGKLSFDLHWIEEAGGVSNLDILF 865
           DGKLSFDLHWIEEAGGVSNLDI F
Sbjct: 841 DGKLSFDLHWIEEAGGVSNLDIFF 864

BLAST of HG10023033 vs. ExPASy TrEMBL
Match: A0A5A7TXN2 (Stachyose synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001820 PE=3 SV=1)

HSP 1 Score: 1753.4 bits (4540), Expect = 0.0e+00
Identity = 830/864 (96.06%), Postives = 853/864 (98.73%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKS+GLENLIDFS+GKISVKGVP+LSEVP NVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPS+RLTN LGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFD 180
           SDLQMETQWVMLN+PEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGS HVKASSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVG 240
           AIAY+HVSDNPY+LMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAKAIEIEHAEK+RDKAIGSGVT+VSKFET IQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           K+ELL+IFG EEEEES+AI+KGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELLEIFGNEEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THL+SKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720
           NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIK 780
           DQKPEAAPMGNFVEYIVYLNQA QI+HTTPKSEPLKATI PSTFELFNFIPLRKLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQIIHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDS 840
           FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNG E++F W+S
Sbjct: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGTELKFVWNS 840

Query: 841 DGKLSFDLHWIEEAGGVSNLDILF 865
           DGKLSFD+HWIEEAGG+SNLDI F
Sbjct: 841 DGKLSFDVHWIEEAGGISNLDIFF 864

BLAST of HG10023033 vs. ExPASy TrEMBL
Match: A8CM21 (Stachyose synthetase variant 1 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1753.0 bits (4539), Expect = 0.0e+00
Identity = 832/864 (96.30%), Postives = 851/864 (98.50%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKS+GLENLIDFS+GKISVKGVP+LSEVPTNVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPTNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPS+RLTN LGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFD 180
           SDLQMETQWVMLN+PEIKSYVVIIPIIEGSFRSA+HPGTDGQVLICAESGS HVK SSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSAMHPGTDGQVLICAESGSTHVKTSSFD 180

Query: 181 AIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVG 240
           AIAY+HVSDNPY+LMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGV+DFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK
Sbjct: 241 IWNGVSDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKL 360
           FRKYKGGSL GPNAPSFDPKKPKLLIAKAIEIEHAEK+RDKAIGSGVT+VSKFET IQKL
Sbjct: 301 FRKYKGGSLTGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           K+EL  IFGKEEEEESSAI+KGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELHGIFGKEEEEESSAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THL+SKI+PCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIVPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720
           NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIK 780
           DQKPEAAPMGNFVEYIVYLNQA QILHTTPKSEPLKATI PSTFELFNFIPLRKLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDS 840
           FAPIGLTNMFN SGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKC+SNGIEVEFEW S
Sbjct: 781 FAPIGLTNMFNCSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCVSNGIEVEFEWKS 840

Query: 841 DGKLSFDLHWIEEAGGVSNLDILF 865
           DGKLSFDLHWIEEAGGV NLDI F
Sbjct: 841 DGKLSFDLHWIEEAGGVFNLDIFF 864

BLAST of HG10023033 vs. ExPASy TrEMBL
Match: A0A1S3BRI8 (stachyose synthase OS=Cucumis melo OX=3656 GN=LOC103492745 PE=3 SV=1)

HSP 1 Score: 1752.3 bits (4537), Expect = 0.0e+00
Identity = 829/864 (95.95%), Postives = 853/864 (98.73%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDA 60
           MAPPNDPAALNASVLKS+GLENLIDFS+GKISVKGVP+LSEVP NVFFSPFSSISQSSDA
Sbjct: 1   MAPPNDPAALNASVLKSDGLENLIDFSDGKISVKGVPVLSEVPNNVFFSPFSSISQSSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRVHSLSYKGGFLGFDQTQPS+RLTN LGKFKGREFVSVFRFKTWWSTMWVGNSG
Sbjct: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSDRLTNSLGKFKGREFVSVFRFKTWWSTMWVGNSG 120

Query: 121 SDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFD 180
           SDLQMETQWVMLN+PEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGS HVKASSFD
Sbjct: 121 SDLQMETQWVMLNIPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSTHVKASSFD 180

Query: 181 AIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVG 240
           AIAY+HVSDNPY+LMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTV+PVG
Sbjct: 181 AIAYVHVSDNPYRLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPVG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLY FDECEK
Sbjct: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYSFDECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKL 360
           FRKYKGGSL+GPNAPSFDPKKPKLLIAKAIEIEHAEK+RDKAIGSGVT+VSKFET IQKL
Sbjct: 301 FRKYKGGSLMGPNAPSFDPKKPKLLIAKAIEIEHAEKERDKAIGSGVTNVSKFETKIQKL 360

Query: 361 KKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           K+ELL+IFG EEEEES+AI+KGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG
Sbjct: 361 KEELLEIFGNEEEEESTAINKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THL+SKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLNSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720
           NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW
Sbjct: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720

Query: 721 DQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIK 780
           DQKPEAAPMGNFVEYIVYLNQA QI+HTTPKSEPLKATI PSTFELFNFIPLRKLGSNIK
Sbjct: 721 DQKPEAAPMGNFVEYIVYLNQAEQIIHTTPKSEPLKATIQPSTFELFNFIPLRKLGSNIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDS 840
           FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNG E++F W+S
Sbjct: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGTELKFVWNS 840

Query: 841 DGKLSFDLHWIEEAGGVSNLDILF 865
           DGKLSFD+HWIEEAGG+SNLDI F
Sbjct: 841 DGKLSFDVHWIEEAGGISNLDIFF 864

BLAST of HG10023033 vs. ExPASy TrEMBL
Match: A0A6J1D420 (stachyose synthase-like OS=Momordica charantia OX=3673 GN=LOC111016788 PE=3 SV=1)

HSP 1 Score: 1662.9 bits (4305), Expect = 0.0e+00
Identity = 782/864 (90.51%), Postives = 825/864 (95.49%), Query Frame = 0

Query: 1   MAPPNDPAALNASVLKSEGLENLIDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDA 60
           MAPPND A L +SVLKSE LENLIDFS GKISVKGVPLLSEVP+NVFFSPFSSI Q SDA
Sbjct: 1   MAPPNDSANLTSSVLKSESLENLIDFSNGKISVKGVPLLSEVPSNVFFSPFSSICQPSDA 60

Query: 61  PLPLLQRVHSLSYKGGFLGFDQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSG 120
           PLPLLQRV+ LS+KGGFLGFDQT+P++RLTN LGKFKGREFVSVFRFKTWWSTMW+GNSG
Sbjct: 61  PLPLLQRVNGLSHKGGFLGFDQTRPADRLTNSLGKFKGREFVSVFRFKTWWSTMWIGNSG 120

Query: 121 SDLQMETQWVMLNVPEIKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFD 180
           SDLQMETQWV+LNVPEIKSYVV IPIIEGSFRSALHPGTDGQVLICAESGS HVK SSFD
Sbjct: 121 SDLQMETQWVILNVPEIKSYVVFIPIIEGSFRSALHPGTDGQVLICAESGSTHVKVSSFD 180

Query: 181 AIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVG 240
           AIAY+HVSDNPYKLMKEAYAA+RVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTV+P+G
Sbjct: 181 AIAYVHVSDNPYKLMKEAYAAIRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVDPIG 240

Query: 241 IWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEK 300
           IWNGVNDF EGGISPRFLIIDDGWQSIN+D EDP RD KNL+LGGTQMT+RLYRF+ECEK
Sbjct: 241 IWNGVNDFAEGGISPRFLIIDDGWQSINMDDEDPNRDGKNLILGGTQMTSRLYRFEECEK 300

Query: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKL 360
           FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIE+EHAE+DRD+AI SGV D SKFE  IQKL
Sbjct: 301 FRKYKGGSLLGPNAPSFDPKKPKLLIAKAIELEHAERDRDEAIASGVNDTSKFEIKIQKL 360

Query: 361 KKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAG 420
           K+E+ +IFGK E+EES A++KGC+SCSCKADNSGMKAFTRDL+TKFKGLDDIFVWHALAG
Sbjct: 361 KEEVDEIFGK-EDEESGAVTKGCSSCSCKADNSGMKAFTRDLKTKFKGLDDIFVWHALAG 420

Query: 421 AWGGVRPGSTHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSY 480
           AWGGVRPG+THLSSKI+PCKLSPGLDGTMTDLAVVKI+EGSIGLVHP+QADDFFDSMHSY
Sbjct: 421 AWGGVRPGATHLSSKIVPCKLSPGLDGTMTDLAVVKIVEGSIGLVHPNQADDFFDSMHSY 480

Query: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCND 540
           LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSL+KNFKGTGLFSSMQQCND
Sbjct: 481 LSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLLKNFKGTGLFSSMQQCND 540

Query: 541 FFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600
           FF+LGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ
Sbjct: 541 FFFLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQ 600

Query: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660
           SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK
Sbjct: 601 SDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFK 660

Query: 661 NPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEW 720
           N LFD KTVLKIWNLNKYGGVIG FNCQGAGWDPKEQRIKGHPECYKP STTVHV+D+EW
Sbjct: 661 NSLFDGKTVLKIWNLNKYGGVIGAFNCQGAGWDPKEQRIKGHPECYKPTSTTVHVSDVEW 720

Query: 721 DQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIK 780
           DQK EAAPMGNFVEYIVYLNQA QILHTTPKSEPLK T+ PSTFELF+FIPLRKLGS+IK
Sbjct: 721 DQKQEAAPMGNFVEYIVYLNQAEQILHTTPKSEPLKVTLQPSTFELFSFIPLRKLGSSIK 780

Query: 781 FAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDS 840
           FAPIGLTNMFNSSGTIQHLKYNE GVELKVKGGG+FLAYS+ SPKKC+SNG+EVEFEWDS
Sbjct: 781 FAPIGLTNMFNSSGTIQHLKYNEKGVELKVKGGGSFLAYSNASPKKCVSNGMEVEFEWDS 840

Query: 841 DGKLSFDLHWIEEAGGVSNLDILF 865
           DGKL FDL W  EAGGVSNLDI F
Sbjct: 841 DGKLGFDLPWNGEAGGVSNLDIFF 863

BLAST of HG10023033 vs. TAIR 10
Match: AT4G01970.1 (stachyose synthase )

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 519/855 (60.70%), Postives = 650/855 (76.02%), Query Frame = 0

Query: 22  NLIDFSEGKISVK-GVPLLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGF 81
           N  + SEG +  K   P+L +VP NV F+PFSS S S+DAPLP+L RV + ++KGGFLGF
Sbjct: 32  NSFNLSEGSLCAKDSTPILFDVPQNVTFTPFSSHSISTDAPLPILLRVQANAHKGGFLGF 91

Query: 82  DQTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVMLNVPEIKSY 141
            +  PS+RLTN LG+F+ REF+S+FRFK WWST W+G SGSDLQ ETQWVML +PEI SY
Sbjct: 92  TKESPSDRLTNSLGRFEDREFLSLFRFKMWWSTAWIGKSGSDLQAETQWVMLKIPEIDSY 151

Query: 142 VVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFDAIAYIHVSDNPYKLMKEAYA 201
           V IIP IEG+FR++L PG  G VLICAESGS  VK SSF +IAYIH+ DNPY LMKEA++
Sbjct: 152 VAIIPTIEGAFRASLTPGEKGNVLICAESGSTKVKESSFKSIAYIHICDNPYNLMKEAFS 211

Query: 202 AVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVGIWNGVNDFVEGGISPRFLII 261
           A+RVH+NTF+LLEEK +  +VDKFGWCTWDA YLTV+P  IW GV +F +GG+ P+F+II
Sbjct: 212 ALRVHMNTFKLLEEKKLPKIVDKFGWCTWDACYLTVDPATIWTGVKEFEDGGVCPKFVII 271

Query: 262 DDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGSLLGPNAPSFDPK 321
           DDGWQSIN DG++  +DA+NLVLGG QMTARL  F EC+KFR YKGGS +  +A  F+P 
Sbjct: 272 DDGWQSINFDGDELDKDAENLVLGGEQMTARLTSFKECKKFRNYKGGSFITSDASHFNPL 331

Query: 322 KPKLLIAKAIEIEHAEKDRDKAI-GSGVTDVSKFETTIQKLKKELLQIFGKEEEEESSAI 381
           KPK+LI KA E   A   R K +  SG  D+++ +  I+ L +EL  +F + E+EES   
Sbjct: 332 KPKMLIYKATERIQAIILRRKLVKESGEQDLTELDEKIKILSEELNAMFDEVEKEESLG- 391

Query: 382 SKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPGS-THLSSKIIP 441
                  S     SGM AFT+DLR +FK LDDI+VWHAL GAW GVRP +   L +K+ P
Sbjct: 392 -------SDDVSGSGMAAFTKDLRLRFKSLDDIYVWHALCGAWNGVRPETMMDLKAKVAP 451

Query: 442 CKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITGVKVDVMHTLE 501
            +LSP L  TM DLAV K++E  IGLVHP +A +F+DSMHSYL+ VG+TG K+DV  TLE
Sbjct: 452 FELSPSLGATMADLAVDKVVEAGIGLVHPSKAHEFYDSMHSYLASVGVTGAKIDVFQTLE 511

Query: 502 YVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYLGTKQNSIGRVGDDF 561
            ++EE+GGRV+LAKAYY GLT S++KNF GT + +SMQQCN+FF+L TKQ SIGRVGDDF
Sbjct: 512 SLAEEHGGRVELAKAYYDGLTESMIKNFNGTDVIASMQQCNEFFFLATKQISIGRVGDDF 571

Query: 562 WFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKFHAGSRAICGG 621
           W+QDP GDP GVYWLQGVHMIHC+YNS+WMGQ+IQPDWDMFQSDH+CA++HA SRAICGG
Sbjct: 572 WWQDPYGDPQGVYWLQGVHMIHCSYNSIWMGQMIQPDWDMFQSDHVCAEYHAASRAICGG 631

Query: 622 PVYVSDSVG--GHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDNKTVLKIWNLN 681
           PVY+SD +G   HNFDLIK+L + DGTIPRC H+ALPTRD LFKNPLFD +++LKI+N N
Sbjct: 632 PVYLSDHLGKASHNFDLIKKLAFFDGTIPRCVHYALPTRDSLFKNPLFDKESILKIFNFN 691

Query: 682 KYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEWDQKPEAAPMGNFV--- 741
           K+GGVIGTFNCQGAGW P+E R KG+ ECY  +S TVHV+DIEWDQ PEAA  G+ V   
Sbjct: 692 KFGGVIGTFNCQGAGWSPEEHRFKGYKECYTTVSGTVHVSDIEWDQNPEAA--GSQVTYT 751

Query: 742 -EYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKL-GSNIKFAPIGLTNMFN 801
            +Y+VY  Q+ +IL    KSE +K T+ PS F+L +F+P+ +L  S ++FAP+GL NMFN
Sbjct: 752 GDYLVYKQQSEEILFMNSKSEAMKITLEPSAFDLLSFVPVTELVSSGVRFAPLGLINMFN 811

Query: 802 SSGTIQHLKY-NENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWDSD-GKLSFDLH 861
             GT+Q +K   +N + + VKG G F+AYSS +P KC  N  E EF+W+ + GKLSF + 
Sbjct: 812 CVGTVQDMKVTGDNSIRVDVKGEGRFMAYSSSAPVKCYLNDKEAEFKWEEETGKLSFFVP 871

Query: 862 WIEEAGGVSNLDILF 865
           W+EE+GG+S+L   F
Sbjct: 872 WVEESGGISHLSFTF 876

BLAST of HG10023033 vs. TAIR 10
Match: AT5G40390.1 (Raffinose synthase family protein )

HSP 1 Score: 732.3 bits (1889), Expect = 4.7e-211
Identity = 375/867 (43.25%), Postives = 513/867 (59.17%), Query Frame = 0

Query: 16  KSEGLENLIDFSE------GKISVKGVPLLSEVPTNVFFSPFSSISQSSDAPLPLLQRVH 75
           KS+   N +DF+E        +   G  +L++VP NV  +    +      PL +     
Sbjct: 8   KSDSGINGVDFTEKFRLEDSTLLANGQVVLTDVPVNVTLTSSPYLVDKDGVPLDV----- 67

Query: 76  SLSYKGGFLGFD-QTQPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQ 135
                G F+GF+   +P       +GK K   F+S+FRFK WW+T WVG++G D++ ETQ
Sbjct: 68  ---SAGSFIGFNLDGEPKSHHVASIGKLKNIRFMSIFRFKVWWTTHWVGSNGRDIENETQ 127

Query: 136 WVMLNVPEIKS---------YVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSF 195
            ++L+     S         YV+++P++EGSFRS+   G D  V +C ESGS  V  S F
Sbjct: 128 IIILDQSGSDSGPGSGSGRPYVLLLPLLEGSFRSSFQSGEDDDVAVCVESGSTEVTGSEF 187

Query: 196 DAIAYIHVSDNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPV 255
             I Y+H  D+P+KL+K+A   +RVH+NTF+LLEEK    +VDKFGWCTWDAFYLTV P 
Sbjct: 188 RQIVYVHAGDDPFKLVKDAMKVIRVHMNTFKLLEEKSPPGIVDKFGWCTWDAFYLTVNPD 247

Query: 256 GIWNGVNDFVEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECE 315
           G+  GV   V+GG  P  ++IDDGWQSI  D +    +  N+ + G QM  RL +F+E  
Sbjct: 248 GVHKGVKCLVDGGCPPGLVLIDDGWQSIGHDSDGIDVEGMNITVAGEQMPCRLLKFEENH 307

Query: 316 KFRKYKGGSLLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQK 375
           KF+ Y                              + KD+                    
Sbjct: 308 KFKDYV-----------------------------SPKDQ-------------------- 367

Query: 376 LKKELLQIFGKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALA 435
                                          ++ GMKAF RDL+ +F  +D I+VWHAL 
Sbjct: 368 -------------------------------NDVGMKAFVRDLKDEFSTVDYIYVWHALC 427

Query: 436 GAWGGVRPGSTHL-SSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMH 495
           G WGG+RP +  L  S II  +LSPGL  TM DLAV KIIE  IG   PD A +F++ +H
Sbjct: 428 GYWGGLRPEAPALPPSTIIRPELSPGLKLTMEDLAVDKIIETGIGFASPDLAKEFYEGLH 487

Query: 496 SYLSKVGITGVKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQC 555
           S+L   GI GVKVDV+H LE + ++YGGRVDLAKAY+K LT+S+ K+F G G+ +SM+ C
Sbjct: 488 SHLQNAGIDGVKVDVIHILEMLCQKYGGRVDLAKAYFKALTSSVNKHFNGNGVIASMEHC 547

Query: 556 NDFFYLGTKQNSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDM 615
           NDF +LGT+  S+GRVGDDFW  DP+GDP G +WLQG HM+HCAYNS+WMG  IQPDWDM
Sbjct: 548 NDFMFLGTEAISLGRVGDDFWCTDPSGDPNGTFWLQGCHMVHCAYNSLWMGNFIQPDWDM 607

Query: 616 FQSDHLCAKFHAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCL 675
           FQS H CA+FHA SRAI GGP+Y+SD VG H+FDL+K+LV P+G+I RC+++ALPTRD L
Sbjct: 608 FQSTHPCAEFHAASRAISGGPIYISDCVGKHDFDLLKRLVLPNGSILRCEYYALPTRDRL 667

Query: 676 FKNPLFDNKTVLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDI 735
           F++PL D KT+LKIWNLNKY GVIG FNCQG GW  + +R +   EC   ++ T    D+
Sbjct: 668 FEDPLHDGKTMLKIWNLNKYTGVIGAFNCQGGGWCRETRRNQCFSECVNTLTATTSPKDV 727

Query: 736 EWDQKPEAAPMGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKL-GS 795
           EW+       + N  E+ ++L+Q+ ++L  +  ++ L+ T+ P  FEL    P+  + G+
Sbjct: 728 EWNSGSSPISIANVEEFALFLSQSKKLL-LSGLNDDLELTLEPFKFELITVSPVVTIEGN 783

Query: 796 NIKFAPIGLTNMFNSSGTIQHLKYNENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFE 855
           +++FAPIGL NM N+SG I+ L YN+  VE+ V G G F  Y+S  P  CL +G  VEF 
Sbjct: 788 SVRFAPIGLVNMLNTSGAIRSLVYNDESVEVGVFGAGEFRVYASKKPVSCLIDGEVVEFG 783

Query: 856 WDSDGKLSFDLHWIEEAGGVSNLDILF 865
           ++ D  +   + W     G+S++  LF
Sbjct: 848 YE-DSMVMVQVPW-SGPDGLSSIQYLF 783

BLAST of HG10023033 vs. TAIR 10
Match: AT5G20250.1 (Raffinose synthase family protein )

HSP 1 Score: 472.6 bits (1215), Expect = 6.7e-133
Identity = 280/843 (33.21%), Postives = 410/843 (48.64%), Query Frame = 0

Query: 24  IDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGFDQT 83
           +  S+G + +K   +L+ VP NV      + S S   P+           +G F+G    
Sbjct: 7   VRISDGNLIIKNRTILTGVPDNVI-----TTSASEAGPV-----------EGVFVGAVFN 66

Query: 84  QPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVMLNVPE------- 143
           +   +   P+G  +   F+S FRFK WW    +G  G D+  ETQ++++   +       
Sbjct: 67  KEESKHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESD 126

Query: 144 --------IKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFDAIAYIHVS 203
                    K Y V +P+IEGSFRS L    + +V +C ESG    K SSF    YIH  
Sbjct: 127 GANGVECNQKVYTVFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYIHAG 186

Query: 204 DNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVGIWNGVNDF 263
            +P++ + +A   V++HLN+FR   EK +  +VD FGWCTWDAFY  V   G+  G+   
Sbjct: 187 TDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSL 246

Query: 264 VEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGS 323
             GG  P+F+IIDDGWQS+  D      D K           RL    E EKF+K     
Sbjct: 247 AAGGTPPKFVIIDDGWQSVERDATVEAGDEKK-----ESPIFRLTGIKENEKFKK----- 306

Query: 324 LLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKLKKELLQIF 383
                    DP                                                 
Sbjct: 307 -------KDDP------------------------------------------------- 366

Query: 384 GKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPG 443
                                  N G+K   +  + K  GL  ++VWHA+ G WGGVRPG
Sbjct: 367 -----------------------NVGIKNIVKIAKEK-HGLKYVYVWHAITGYWGGVRPG 426

Query: 444 STHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITG 503
             + S    P      ++   T    V  ++G +GLV P +   F++ +HSYL+  G+ G
Sbjct: 427 EEYGSVMKYPNMSKGVVENDPTWKTDVMTLQG-LGLVSPKKVYKFYNELHSYLADAGVDG 486

Query: 504 VKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYLGTKQ 563
           VKVDV   LE +    GGRV+L + +++ L +S+ KNF   G  + M    D  Y  +KQ
Sbjct: 487 VKVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC-SKQ 546

Query: 564 NSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKF 623
            ++ R  DDF+ +DP            +H+   AYNS+++G+ +QPDWDMF S H  A++
Sbjct: 547 AAVIRASDDFYPRDPVS--------HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEY 606

Query: 624 HAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDNKT 683
           HA +RAI GGP+YVSDS G HNF+L+++LV PDG+I R +    PTRDCLF +P  D  +
Sbjct: 607 HASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARDGVS 666

Query: 684 VLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEWDQKPEAAP 743
           +LKIWN+NKY GV+G +NCQGA W   E++   H      ++ ++   D+    +    P
Sbjct: 667 LLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEASTDP 726

Query: 744 MGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIKFAPIGLTN 803
                +  VY    G+++   P +  L  ++     E+F   P+  L   + FAPIGL N
Sbjct: 727 TTWNGDCAVYSQSRGELI-VMPYNVSLPVSLKIREHEIFTVSPISHLVDGVSFAPIGLVN 732

Query: 804 MFNSSGTIQHLKY--NENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWD-SDGKLS 849
           M+NS G I+ L+Y   +  V ++VKG G F +YSS  PK+C+    E+ FE+D S G ++
Sbjct: 787 MYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYDSSSGLVT 732

BLAST of HG10023033 vs. TAIR 10
Match: AT5G20250.2 (Raffinose synthase family protein )

HSP 1 Score: 472.6 bits (1215), Expect = 6.7e-133
Identity = 280/843 (33.21%), Postives = 410/843 (48.64%), Query Frame = 0

Query: 24  IDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGFDQT 83
           +  S+G + +K   +L+ VP NV      + S S   P+           +G F+G    
Sbjct: 7   VRISDGNLIIKNRTILTGVPDNVI-----TTSASEAGPV-----------EGVFVGAVFN 66

Query: 84  QPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVMLNVPE------- 143
           +   +   P+G  +   F+S FRFK WW    +G  G D+  ETQ++++   +       
Sbjct: 67  KEESKHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESD 126

Query: 144 --------IKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFDAIAYIHVS 203
                    K Y V +P+IEGSFRS L    + +V +C ESG    K SSF    YIH  
Sbjct: 127 GANGVECNQKVYTVFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYIHAG 186

Query: 204 DNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVGIWNGVNDF 263
            +P++ + +A   V++HLN+FR   EK +  +VD FGWCTWDAFY  V   G+  G+   
Sbjct: 187 TDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSL 246

Query: 264 VEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGS 323
             GG  P+F+IIDDGWQS+  D      D K           RL    E EKF+K     
Sbjct: 247 AAGGTPPKFVIIDDGWQSVERDATVEAGDEKK-----ESPIFRLTGIKENEKFKK----- 306

Query: 324 LLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKLKKELLQIF 383
                    DP                                                 
Sbjct: 307 -------KDDP------------------------------------------------- 366

Query: 384 GKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPG 443
                                  N G+K   +  + K  GL  ++VWHA+ G WGGVRPG
Sbjct: 367 -----------------------NVGIKNIVKIAKEK-HGLKYVYVWHAITGYWGGVRPG 426

Query: 444 STHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITG 503
             + S    P      ++   T    V  ++G +GLV P +   F++ +HSYL+  G+ G
Sbjct: 427 EEYGSVMKYPNMSKGVVENDPTWKTDVMTLQG-LGLVSPKKVYKFYNELHSYLADAGVDG 486

Query: 504 VKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYLGTKQ 563
           VKVDV   LE +    GGRV+L + +++ L +S+ KNF   G  + M    D  Y  +KQ
Sbjct: 487 VKVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC-SKQ 546

Query: 564 NSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKF 623
            ++ R  DDF+ +DP            +H+   AYNS+++G+ +QPDWDMF S H  A++
Sbjct: 547 AAVIRASDDFYPRDPVS--------HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEY 606

Query: 624 HAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDNKT 683
           HA +RAI GGP+YVSDS G HNF+L+++LV PDG+I R +    PTRDCLF +P  D  +
Sbjct: 607 HASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARDGVS 666

Query: 684 VLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEWDQKPEAAP 743
           +LKIWN+NKY GV+G +NCQGA W   E++   H      ++ ++   D+    +    P
Sbjct: 667 LLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEASTDP 726

Query: 744 MGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIKFAPIGLTN 803
                +  VY    G+++   P +  L  ++     E+F   P+  L   + FAPIGL N
Sbjct: 727 TTWNGDCAVYSQSRGELI-VMPYNVSLPVSLKIREHEIFTVSPISHLVDGVSFAPIGLVN 732

Query: 804 MFNSSGTIQHLKY--NENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWD-SDGKLS 849
           M+NS G I+ L+Y   +  V ++VKG G F +YSS  PK+C+    E+ FE+D S G ++
Sbjct: 787 MYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYDSSSGLVT 732

BLAST of HG10023033 vs. TAIR 10
Match: AT5G20250.3 (Raffinose synthase family protein )

HSP 1 Score: 472.6 bits (1215), Expect = 6.7e-133
Identity = 280/843 (33.21%), Postives = 410/843 (48.64%), Query Frame = 0

Query: 24  IDFSEGKISVKGVPLLSEVPTNVFFSPFSSISQSSDAPLPLLQRVHSLSYKGGFLGFDQT 83
           +  S+G + +K   +L+ VP NV      + S S   P+           +G F+G    
Sbjct: 7   VRISDGNLIIKNRTILTGVPDNVI-----TTSASEAGPV-----------EGVFVGAVFN 66

Query: 84  QPSERLTNPLGKFKGREFVSVFRFKTWWSTMWVGNSGSDLQMETQWVMLNVPE------- 143
           +   +   P+G  +   F+S FRFK WW    +G  G D+  ETQ++++   +       
Sbjct: 67  KEESKHIVPIGTLRNSRFMSCFRFKLWWMAQRMGEMGRDIPYETQFLLVESNDGSHLESD 126

Query: 144 --------IKSYVVIIPIIEGSFRSALHPGTDGQVLICAESGSAHVKASSFDAIAYIHVS 203
                    K Y V +P+IEGSFRS L    + +V +C ESG    K SSF    YIH  
Sbjct: 127 GANGVECNQKVYTVFLPLIEGSFRSCLQGNVNDEVELCLESGDVDTKRSSFTHSLYIHAG 186

Query: 204 DNPYKLMKEAYAAVRVHLNTFRLLEEKPVTHLVDKFGWCTWDAFYLTVEPVGIWNGVNDF 263
            +P++ + +A   V++HLN+FR   EK +  +VD FGWCTWDAFY  V   G+  G+   
Sbjct: 187 TDPFQTITDAIRTVKLHLNSFRQRHEKKLPGIVDYFGWCTWDAFYQEVTQEGVEAGLKSL 246

Query: 264 VEGGISPRFLIIDDGWQSINLDGEDPTRDAKNLVLGGTQMTARLYRFDECEKFRKYKGGS 323
             GG  P+F+IIDDGWQS+  D      D K           RL    E EKF+K     
Sbjct: 247 AAGGTPPKFVIIDDGWQSVERDATVEAGDEKK-----ESPIFRLTGIKENEKFKK----- 306

Query: 324 LLGPNAPSFDPKKPKLLIAKAIEIEHAEKDRDKAIGSGVTDVSKFETTIQKLKKELLQIF 383
                    DP                                                 
Sbjct: 307 -------KDDP------------------------------------------------- 366

Query: 384 GKEEEEESSAISKGCTSCSCKADNSGMKAFTRDLRTKFKGLDDIFVWHALAGAWGGVRPG 443
                                  N G+K   +  + K  GL  ++VWHA+ G WGGVRPG
Sbjct: 367 -----------------------NVGIKNIVKIAKEK-HGLKYVYVWHAITGYWGGVRPG 426

Query: 444 STHLSSKIIPCKLSPGLDGTMTDLAVVKIIEGSIGLVHPDQADDFFDSMHSYLSKVGITG 503
             + S    P      ++   T    V  ++G +GLV P +   F++ +HSYL+  G+ G
Sbjct: 427 EEYGSVMKYPNMSKGVVENDPTWKTDVMTLQG-LGLVSPKKVYKFYNELHSYLADAGVDG 486

Query: 504 VKVDVMHTLEYVSEEYGGRVDLAKAYYKGLTNSLVKNFKGTGLFSSMQQCNDFFYLGTKQ 563
           VKVDV   LE +    GGRV+L + +++ L +S+ KNF   G  + M    D  Y  +KQ
Sbjct: 487 VKVDVQCVLETLGGGLGGRVELTRQFHQALDSSVAKNFPDNGCIACMSHNTDALYC-SKQ 546

Query: 564 NSIGRVGDDFWFQDPNGDPMGVYWLQGVHMIHCAYNSMWMGQIIQPDWDMFQSDHLCAKF 623
            ++ R  DDF+ +DP            +H+   AYNS+++G+ +QPDWDMF S H  A++
Sbjct: 547 AAVIRASDDFYPRDPVS--------HTIHIASVAYNSVFLGEFMQPDWDMFHSVHPAAEY 606

Query: 624 HAGSRAICGGPVYVSDSVGGHNFDLIKQLVYPDGTIPRCQHFALPTRDCLFKNPLFDNKT 683
           HA +RAI GGP+YVSDS G HNF+L+++LV PDG+I R +    PTRDCLF +P  D  +
Sbjct: 607 HASARAISGGPLYVSDSPGKHNFELLRKLVLPDGSILRARLPGRPTRDCLFADPARDGVS 666

Query: 684 VLKIWNLNKYGGVIGTFNCQGAGWDPKEQRIKGHPECYKPMSTTVHVNDIEWDQKPEAAP 743
           +LKIWN+NKY GV+G +NCQGA W   E++   H      ++ ++   D+    +    P
Sbjct: 667 LLKIWNMNKYTGVLGVYNCQGAAWSSTERKNIFHQTKTDSLTGSIRGRDVHSISEASTDP 726

Query: 744 MGNFVEYIVYLNQAGQILHTTPKSEPLKATILPSTFELFNFIPLRKLGSNIKFAPIGLTN 803
                +  VY    G+++   P +  L  ++     E+F   P+  L   + FAPIGL N
Sbjct: 727 TTWNGDCAVYSQSRGELI-VMPYNVSLPVSLKIREHEIFTVSPISHLVDGVSFAPIGLVN 732

Query: 804 MFNSSGTIQHLKY--NENGVELKVKGGGNFLAYSSGSPKKCLSNGIEVEFEWD-SDGKLS 849
           M+NS G I+ L+Y   +  V ++VKG G F +YSS  PK+C+    E+ FE+D S G ++
Sbjct: 787 MYNSGGAIEGLRYEAEKMKVVMEVKGCGKFGSYSSVKPKRCVVESNEIAFEYDSSSGLVT 732