HG10009094 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10009094 vs. NCBI nr
Match: XP_008437346.1 (PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Cucumis melo])

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 607/833 (72.87%), Postives = 662/833 (79.47%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +D+AIANN+VPSKAH LP LFKQLCQ+KHS R KAALMVLM+TIKNACK +WFSEKDAEE
Sbjct: 47  IDYAIANNAVPSKAHSLPILFKQLCQLKHSHRSKAALMVLMLTIKNACKVRWFSEKDAEE 106

Query: 61  LYSLANEIGNDFFGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAIDF 120
           L  LANEIGNDFFGD NIGQ+NSLTTITTVMER+FP LKLGQIVAS+EVKPGYGV+A+DF
Sbjct: 107 LQRLANEIGNDFFGDMNIGQANSLTTITTVMERYFPCLKLGQIVASLEVKPGYGVYALDF 166

Query: 121 NILKTIQYAPQEKI---------------------------------------DTGPQLP 180
           NI +TIQ APQEK+                                       DTGPQLP
Sbjct: 167 NISRTIQCAPQEKLRLFVIQKDNTETSACMISPPQVNFLVNGRGVNGRINTHMDTGPQLP 226

Query: 181 TNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDTGYINL 240
           TNITHMLKLGSNLLQAIGSFNGHYV+AVAI GTAP+P+SSVLQDH+QPV+ST+D+     
Sbjct: 227 TNITHMLKLGSNLLQAIGSFNGHYVLAVAITGTAPSPNSSVLQDHVQPVVSTLDSD---- 286

Query: 241 VSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQ------------- 300
            S+ + GP+ ++            L   + YTRIK+PVKG SCKHLQ             
Sbjct: 287 -SDIIEGPSRIS------------LNCPISYTRIKIPVKGCSCKHLQCFDFDNFIDINSR 346

Query: 301 --------------------------VIREVAENVTEVIISADGSWKAILENDYGDGRPL 360
                                     VIREVAENVTEVIISADGSWKAILEND GDGR L
Sbjct: 347 RPSWRCPHCNQYICFLDIRVDQNMLKVIREVAENVTEVIISADGSWKAILENDNGDGRSL 406

Query: 361 DDSLNHQNERAQEESTAPPDVLDLTEVDDDMDICNLETEDRKPCLGNKNQPVSSTLDISS 420
           DDSLNHQ+ERAQEES+AP DVLDLTEV DDMDI + E EDRKPCLGNKNQPVSS+LD+SS
Sbjct: 407 DDSLNHQSERAQEESSAPSDVLDLTEVGDDMDIFDSEIEDRKPCLGNKNQPVSSSLDMSS 466

Query: 421 GMNMNSLNQNLAAVLNDDFWSGIVTDGILTSSAGSDAPMGNSTPPPGFAGIMQSTVLTDV 480
           GMNMNS +QNL+AV+ DDFWS +  DG+L SSAG DAPM NST PPGF  IMQS VLTDV
Sbjct: 467 GMNMNSFSQNLSAVVEDDFWSRL--DGVLISSAGLDAPMVNSTYPPGFTNIMQSAVLTDV 526

Query: 481 VPPVLNHGAGVPGHANFSSPAFYDQNNFQIQISNSNENNQYGRMTSIARPVSRTPIAVQA 540
           V PVLNHG GV GHANFSSPAFY+QNN  IQ+SNSNENNQYGR+TSI+ P SRTPIAVQA
Sbjct: 527 VQPVLNHGVGVLGHANFSSPAFYNQNNMHIQVSNSNENNQYGRVTSISIPASRTPIAVQA 586

Query: 541 LPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRDGLNTLSRDLERRQQFSRHHGDSHHAT 600
           LPAQS AAGQQYSSRTPII S+PQVGQSIPINRDGLN LSRDLERRQQFSRHHGDSHHAT
Sbjct: 587 LPAQSHAAGQQYSSRTPII-SSPQVGQSIPINRDGLNALSRDLERRQQFSRHHGDSHHAT 646

Query: 601 NLAPCHHSQTMQNRDPQDRSFTPGQSVQASTAVRPSTGLLTDFQNPHLQQALNLRISHLR 660
           NLA  HH QT+QNRDPQDRSFT GQS+Q S   RPSTGLLTDFQNPHLQQAL+ R+ HL+
Sbjct: 647 NLASFHHPQTVQNRDPQDRSFTTGQSIQTSNGARPSTGLLTDFQNPHLQQALS-RMPHLQ 706

Query: 661 NQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAATPNSQHARMMAASQRTEMMRQSSAMSS 720
           NQNSSSVRPSL FSRPMSQV GGGYGGS YT  TPNSQH+RMMAASQR EMMRQS +MS 
Sbjct: 707 NQNSSSVRPSLSFSRPMSQV-GGGYGGSTYTTVTPNSQHSRMMAASQRAEMMRQSPSMSL 766

Query: 721 QNQTSRSAHSLQTTPDGLRRPSGELRNV-GVSQSVTMAAGSVDLSVEQNWQPVGRMRGSL 755
           QNQTSRSAHSLQTTPDGLRRPSG+LRNV GVSQSVT+AAGSVDLS EQNWQP GRMRGSL
Sbjct: 767 QNQTSRSAHSLQTTPDGLRRPSGDLRNVGGVSQSVTIAAGSVDLSAEQNWQPAGRMRGSL 826

BLAST of HG10009094 vs. NCBI nr
Match: XP_011654714.1 (E4 SUMO-protein ligase PIAL2 isoform X1 [Cucumis sativus] >KAE8647846.1 hypothetical protein Csa_000250 [Cucumis sativus])

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 598/832 (71.88%), Postives = 656/832 (78.85%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +D+AIANN+VPSKAH LP+L KQLCQ+KHS R KAALMVLM+TIKNACK +WFSEKDAEE
Sbjct: 47  IDYAIANNAVPSKAHSLPSLVKQLCQLKHSHRSKAALMVLMLTIKNACKVRWFSEKDAEE 106

Query: 61  LYSLANEIGNDFFGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAIDF 120
           L  LANEIGNDFFGDTNIGQ+NSLTTITTVMER+FP LKLGQIVAS+EVKPGYGV+A+DF
Sbjct: 107 LQRLANEIGNDFFGDTNIGQANSLTTITTVMERYFPCLKLGQIVASLEVKPGYGVYALDF 166

Query: 121 NILKTIQYAPQEKI---------------------------------------DTGPQLP 180
           NI +T+QYA QEK+                                       DTGPQLP
Sbjct: 167 NISRTVQYASQEKLRLFVIQKDNTETSACIISPPQVNFLVNGRGINGRINTHMDTGPQLP 226

Query: 181 TNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDTGYINL 240
           TNITHMLKLGSNLLQA+GSFNGHYV+A+AI GTAP+PDSSVL DHIQP++ST+D+     
Sbjct: 227 TNITHMLKLGSNLLQAVGSFNGHYVLAIAITGTAPSPDSSVLHDHIQPIVSTLDSD---- 286

Query: 241 VSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQ------------- 300
            S+ + GP+ ++            L   + YTRIK+PVKG SCKHLQ             
Sbjct: 287 -SDIIEGPSRIS------------LNCPISYTRIKIPVKGCSCKHLQCFDFDNFIDINSR 346

Query: 301 --------------------------VIREVAENVTEVIISADGSWKAILENDYGDGRPL 360
                                     VIREVAENVTEVIIS DGSWKAILEND GDGR L
Sbjct: 347 RPSWRCPHCNQYICFLDIRVDRNMLKVIREVAENVTEVIISVDGSWKAILENDNGDGRSL 406

Query: 361 DDSLNHQNERAQEESTAPPDVLDLTEVDDDMDICNLETEDRKPCLGNKNQPVSSTLDISS 420
           +DSLNHQNERAQEES A PDVLD TEV DDMDI N E EDRKPCLGNKNQ VSS+LD+SS
Sbjct: 407 NDSLNHQNERAQEESAASPDVLDHTEVGDDMDIFNSEIEDRKPCLGNKNQRVSSSLDMSS 466

Query: 421 GMNMNSLNQNLAAVLNDDFWSGIVTDGILTSSAGSDAPMGNSTPPPGFAGIMQSTVLTDV 480
           GMNMNS +QNL+AV++DD WS I  DG+L S+AG DAPM NST PPGF G MQS VLTD 
Sbjct: 467 GMNMNSFSQNLSAVMDDDIWSRI--DGVLISTAGLDAPMVNSTYPPGFTGTMQSAVLTDA 526

Query: 481 VPPVLNHGAGVPGHANFSSPAFYDQNNFQIQISNSNENNQYGRMTSIARPVSRTPIAVQA 540
           V PVLNHG GV GHANF SPAFY+QNN QIQ+SNSNENNQYGR+TSI+RPVSRTP+AVQA
Sbjct: 527 VQPVLNHGVGVSGHANFPSPAFYNQNNVQIQVSNSNENNQYGRVTSISRPVSRTPVAVQA 586

Query: 541 LPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRDGLNTLSRDLERRQQFSRHHGDSHHAT 600
           LPAQS AAGQQYSSRTPII S+PQVGQSIPINRDGLN LSRDLERRQQFSRHHGDSHH+T
Sbjct: 587 LPAQSHAAGQQYSSRTPII-SSPQVGQSIPINRDGLNALSRDLERRQQFSRHHGDSHHST 646

Query: 601 NLAPCHHSQTMQNRDPQDRSFTPGQSVQASTAVRPSTGLLTDFQNPHLQQALNLRISHLR 660
           NLA  HH QT+QNRDPQDRSFT GQS+Q S+  RPS GLL DFQNPHLQQALN+R+ HL+
Sbjct: 647 NLASFHHPQTVQNRDPQDRSFTTGQSIQTSSGARPSPGLLADFQNPHLQQALNMRMPHLQ 706

Query: 661 NQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAATPNSQHARMMAASQRTEMMRQSSAMSS 720
           NQNSSSVR SL FSRPMSQV GGGYGGS YT  TPNSQHARM+AASQR EMMRQS  MS 
Sbjct: 707 NQNSSSVRTSLSFSRPMSQV-GGGYGGSTYTTVTPNSQHARMLAASQRVEMMRQSPPMSL 766

Query: 721 QNQTSRSAHSLQTTPDGLRRPSGELRNVGVSQSVTMAAGSVDLSVEQNWQPVGRMRGSLS 755
            NQTSRSAHSLQTTPDGLRRPSG+LRNVGVSQSVTMAAGSVDLS EQNWQP GRMRGSLS
Sbjct: 767 HNQTSRSAHSLQTTPDGLRRPSGDLRNVGVSQSVTMAAGSVDLSAEQNWQPAGRMRGSLS 826

BLAST of HG10009094 vs. NCBI nr
Match: XP_022928991.1 (E4 SUMO-protein ligase PIAL2-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 590/799 (73.84%), Postives = 643/799 (80.48%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +DFAIAN+ VPSKA GLP+L KQ+CQ KHS  LKAA+MVLMI  KNACK KWFSEK+AEE
Sbjct: 47  IDFAIANDFVPSKAQGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEE 106

Query: 61  LYSLANEIGNDFFGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAIDF 120
           LYSLANEIG+DFF DTN G SNSLTTITTVMERFFPRLKLGQIV S EVKPGYGVFA DF
Sbjct: 107 LYSLANEIGSDFFVDTNTGPSNSLTTITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDF 166

Query: 121 NILKTIQYAPQEKI--------------------------------------------DT 180
           NI KTIQYAPQEKI                                            DT
Sbjct: 167 NISKTIQYAPQEKIRLFVAQKDNTETSACIISPPQLPCQWEGSQWKDKYIHASFLFNKDT 226

Query: 181 GPQLPTNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDT 240
           GPQLPTN+THMLKLGSNLLQ IGSFNGHYVIAVA+MG+AP+PDSSVLQDH QPV+STVD+
Sbjct: 227 GPQLPTNVTHMLKLGSNLLQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPVVSTVDS 286

Query: 241 GYINLVSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQVIREVAEN 300
                 S+ + GP+ ++            L   + YTRIKVPVKGRSCKHLQVIREVAEN
Sbjct: 287 D-----SDIIEGPSRIS------------LNCPISYTRIKVPVKGRSCKHLQVIREVAEN 346

Query: 301 VTEVIISADGSWKAILENDYGDGRPLDDSLNHQNERAQEESTAPPDVLDLTEVDDDMDIC 360
           VTEVIISADGSWKAILEND GDGRPLDDSLN QNERAQ+ESTAPPDVLDLTEVDDDM+IC
Sbjct: 347 VTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDDDMNIC 406

Query: 361 NLETEDRKPCLGNKNQPVSSTLDISSGMNMNSLNQNLAAVLNDDFWSGIVTDGILTSSAG 420
           NLETEDRKPCLGNKNQPVSS+L+I SGMN NSLNQN +A L+DDFWSG+VTD +L SS  
Sbjct: 407 NLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSGMVTDRLLISSIR 466

Query: 421 SDAPMGNSTPPPGFAGIMQSTVLTDVVPPVLNHGAGVPGHANFSSPAFYDQNNFQIQISN 480
           SDAPMG+ST  P FAG+ QS  LTD V PVLNH  GVPG  NF  PAFYDQNN Q+Q+SN
Sbjct: 467 SDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPAFYDQNNVQVQVSN 526

Query: 481 SNENNQYGRMTSIARPVSRTPIAVQALPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRD 540
           SNE+NQYGRMTSIARPVSRT +A Q LPAQSQ +GQQYSSRT  I SAPQVGQSIPI+RD
Sbjct: 527 SNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTISSAPQVGQSIPISRD 586

Query: 541 GLNTLSRDLERRQQFSRHHGDSHHATNLAPCHHSQTMQNRDPQDRSFTPGQSVQASTAVR 600
           GLNT+SRD ERRQ F RHHGD HHATNLAP      +QNR+PQDRSFTPGQSV+ASTA R
Sbjct: 587 GLNTISRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQSVRASTAQR 646

Query: 601 PSTGLLTDFQNPHLQQALNLRISHLRNQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAAT 660
           PS G+LTDFQNPHLQQ+LNLRISHLRNQN SSVRPSL FSRP SQV GGGYGGSAY A T
Sbjct: 647 PSAGILTDFQNPHLQQSLNLRISHLRNQNPSSVRPSLPFSRPTSQV-GGGYGGSAYPAVT 706

Query: 661 PNSQHARMMAASQRTEMMRQSSAMSSQNQTSRSAHSLQTTPDGLRRPSGELRNV-GVSQS 720
           P++QHARMM ASQR EMMRQSSAMS QNQTSRS H LQTTPDGLRRP+G+LRNV G++QS
Sbjct: 707 PHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLRRPAGDLRNVGGMTQS 766

Query: 721 VTMAAGSVDLSVEQNWQPVGRMRGSLSGRAYSDAYGVIIQPTQAAQSARPPSHLTPTQPS 755
           VTMA+  +D SVEQN QP+GRMRGSLSGRAYSDAYGVIIQPTQ  QS RPPS+LT TQ +
Sbjct: 767 VTMASDLLDPSVEQNRQPIGRMRGSLSGRAYSDAYGVIIQPTQPVQSTRPPSNLTTTQSN 826

BLAST of HG10009094 vs. NCBI nr
Match: XP_023550944.1 (uncharacterized protein LOC111808928 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 588/794 (74.06%), Postives = 641/794 (80.73%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +DFAIAN+ VPSKA GLP+L KQ+CQ KHS  LKAA+MV+MI  KNACK KWFSEK+AEE
Sbjct: 47  IDFAIANDFVPSKAQGLPSLLKQICQKKHSHHLKAAIMVVMIAAKNACKVKWFSEKEAEE 106

Query: 61  LYSLANEIGNDFFGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAIDF 120
           LYSLANEIG+DFF DTN G SN+L TITTVMERFFPRLKLGQIV S EVKPGYGVFA DF
Sbjct: 107 LYSLANEIGSDFFVDTNTGPSNALATITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDF 166

Query: 121 NILKTIQYAPQEKI---------------------------------------DTGPQLP 180
           NI KTIQYAPQEKI                                       DTGPQLP
Sbjct: 167 NISKTIQYAPQEKIRLFVAQKDNTETSACIISPPQVNFLVNGRGVNGRTNIYMDTGPQLP 226

Query: 181 TNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDTGYINL 240
           TN+THMLKLGSNLLQ IGSFNGHYVIAVA+MG+AP+PDSSVLQDH QP +STVD+     
Sbjct: 227 TNVTHMLKLGSNLLQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPAVSTVDSD---- 286

Query: 241 VSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQVIREVAENVTEVI 300
            S+ + GP+ ++            L   + YTRIKVPVKGRSCKHLQVIREVAENVTEVI
Sbjct: 287 -SDIIEGPSRIS------------LNCPISYTRIKVPVKGRSCKHLQVIREVAENVTEVI 346

Query: 301 ISADGSWKAILENDYGDGRPLDDSLNHQNERAQEESTAPPDVLDLTEVDDDMDICNLETE 360
           ISADGSWKAILEND GDGRPLDDSLN QNERA++ESTAPPDVLDLTEVDDDM+ICNLETE
Sbjct: 347 ISADGSWKAILENDCGDGRPLDDSLNQQNERAEQESTAPPDVLDLTEVDDDMNICNLETE 406

Query: 361 DRKPCLGNKNQPVSSTLDISSGMNMNSLNQNLAAVLNDDFWSGIVTDGILTSSAGSDAPM 420
           DRKPCLGNKNQPVSS+L+I SGMN NSLNQN +A L+DDFWSG+VTD +LTSS  SDAPM
Sbjct: 407 DRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSGMVTDRLLTSSIRSDAPM 466

Query: 421 GNSTPPPGFAGIMQSTVLTDVVPPVLNHGAGVPGHANFSSPAFYDQNNFQIQISNSNENN 480
           G+ST  P FAG+ QS  LTD V PVLNH  GVPG  NF  PAFYDQNN Q+Q+SNSNE+N
Sbjct: 467 GSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPAFYDQNNVQVQVSNSNESN 526

Query: 481 QYGRMTSIARPVSRTPIAVQALPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRDGLNTL 540
           QYGRMTSIARPVSRT +A Q LPAQSQ +GQQYSSRT  I SAPQVGQSIPI+RDGLN +
Sbjct: 527 QYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTISSAPQVGQSIPISRDGLNMI 586

Query: 541 SRDLERRQQFSRHHGDSHHATNLAPCHHSQTMQNRDPQDRSFTPGQSVQASTAVRPSTGL 600
           SRD ERRQ F RHHGD HHATNLAP      +QNR+PQD SFTPGQSV+ASTA RPS G+
Sbjct: 587 SRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDCSFTPGQSVRASTAQRPSAGI 646

Query: 601 LTDFQNPHLQQALNLRISHLRNQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAATPNSQH 660
           LTDFQNPHLQQALNLRISHLRNQN SSVRPSL FSRP SQV GGGYGGSAYTA TP++QH
Sbjct: 647 LTDFQNPHLQQALNLRISHLRNQNPSSVRPSLPFSRPTSQV-GGGYGGSAYTAVTPHNQH 706

Query: 661 ARMMAASQRTEMMRQSSAMSSQNQTSRSAHSLQTTPDGLRRPSGELRNV-GVSQSVTMAA 720
           ARMM ASQR EMMRQSSAMS QNQTSRS H LQTTPDGLRRP+GELRNV G++QSVTMA+
Sbjct: 707 ARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLRRPTGELRNVGGMTQSVTMAS 766

Query: 721 GSVDLSVEQNWQPVGRMRGSLSGRAYSDAYGVIIQPTQAAQSARPPSHLTPTQPSAPSMQ 755
             +D SVEQN QP+GRMRGSLSGRAYSDAYGVIIQPTQ  QSARPPS+LT TQ SAPS  
Sbjct: 767 DLLDPSVEQNRQPIGRMRGSLSGRAYSDAYGVIIQPTQPVQSARPPSNLTTTQSSAPSTH 821

BLAST of HG10009094 vs. NCBI nr
Match: XP_022969989.1 (uncharacterized protein LOC111469015 isoform X3 [Cucurbita maxima])

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 587/799 (73.47%), Postives = 642/799 (80.35%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +DFAIAN+ VPSKA GLP+L KQ+CQ KHS  LKAA+MVLMI  KNACK KWFSEK+AEE
Sbjct: 47  IDFAIANDFVPSKAQGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEE 106

Query: 61  LYSLANEIGNDFFGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAIDF 120
           LYSLANEIG+DFF DTN G SNSLTTIT VMERFFPRLKLGQIV S EVKPGYGVFA DF
Sbjct: 107 LYSLANEIGSDFFVDTNTGPSNSLTTITKVMERFFPRLKLGQIVISAEVKPGYGVFAFDF 166

Query: 121 NILKTIQYAPQEKI--------------------------------------------DT 180
           NI KTIQYAPQEKI                                            DT
Sbjct: 167 NISKTIQYAPQEKIRLFVAQKDNTETSACIISPPQLPCQWEGSQWKDKYIHASFLFNKDT 226

Query: 181 GPQLPTNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDT 240
           GPQLPTN+THMLKLGSNLLQ IGSFNGHYVI+VA+MG+AP+PDSSVLQDH QP +STVD+
Sbjct: 227 GPQLPTNVTHMLKLGSNLLQVIGSFNGHYVISVAVMGSAPSPDSSVLQDHEQPAVSTVDS 286

Query: 241 GYINLVSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQVIREVAEN 300
                 S+ + GP+ ++            L   + YTRIKVPVKGRSCKHLQVIREVAEN
Sbjct: 287 D-----SDIIEGPSRIS------------LNCPISYTRIKVPVKGRSCKHLQVIREVAEN 346

Query: 301 VTEVIISADGSWKAILENDYGDGRPLDDSLNHQNERAQEESTAPPDVLDLTEVDDDMDIC 360
           VTEVIISADGSWKAILEND GDGRPLDDSLN QNERAQ+ESTAPPDVLDLTEVDDDM+IC
Sbjct: 347 VTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDDDMNIC 406

Query: 361 NLETEDRKPCLGNKNQPVSSTLDISSGMNMNSLNQNLAAVLNDDFWSGIVTDGILTSSAG 420
           NLETEDRKPCLGNKNQPVSS+L+I SGMN NSLNQN +A L+DDFWS +VTD +LTSS  
Sbjct: 407 NLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSRMVTDRLLTSSIR 466

Query: 421 SDAPMGNSTPPPGFAGIMQSTVLTDVVPPVLNHGAGVPGHANFSSPAFYDQNNFQIQISN 480
           SDAPMG+ST  P FAG+ QS  LTD V PVLNH  GVPG  NF  P+FYDQNN Q+Q+SN
Sbjct: 467 SDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPSFYDQNNVQVQVSN 526

Query: 481 SNENNQYGRMTSIARPVSRTPIAVQALPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRD 540
           SNE+NQYGRMTSIARPVSRT +A Q LPAQSQ +GQQYSSRT  + SAPQVGQSIPI+RD
Sbjct: 527 SNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTVSSAPQVGQSIPISRD 586

Query: 541 GLNTLSRDLERRQQFSRHHGDSHHATNLAPCHHSQTMQNRDPQDRSFTPGQSVQASTAVR 600
           GLNT+SRD E RQ F RHHGD HHATNLAP      +QNR+PQDRSFTPGQSV+ASTA R
Sbjct: 587 GLNTISRDSEMRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQSVRASTAQR 646

Query: 601 PSTGLLTDFQNPHLQQALNLRISHLRNQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAAT 660
           PS G+LTDFQNPHLQQALNLRISHL+NQN SSVRPSL FSRP SQV GGGYGGSAYTA T
Sbjct: 647 PSVGILTDFQNPHLQQALNLRISHLQNQNPSSVRPSLPFSRPTSQV-GGGYGGSAYTAVT 706

Query: 661 PNSQHARMMAASQRTEMMRQSSAMSSQNQTSRSAHSLQTTPDGLRRPSGELRNV-GVSQS 720
           P++QHARMM ASQR EMMRQSSAMS QNQTSRS H LQTTPDGLRRP+GELRNV G++QS
Sbjct: 707 PHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLRRPAGELRNVGGMTQS 766

Query: 721 VTMAAGSVDLSVEQNWQPVGRMRGSLSGRAYSDAYGVIIQPTQAAQSARPPSHLTPTQPS 755
           VTMA+  +D SVEQN QP+GRMRGSLSGRAYSDA+GVIIQPTQ  QSARPPS+LT TQ S
Sbjct: 767 VTMASNLLDPSVEQNRQPIGRMRGSLSGRAYSDAFGVIIQPTQPVQSARPPSNLTTTQSS 826

BLAST of HG10009094 vs. ExPASy Swiss-Prot
Match: F4JYG0 (E4 SUMO-protein ligase PIAL2 OS=Arabidopsis thaliana OX=3702 GN=PIAL2 PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 6.6e-49
Identity = 149/473 (31.50%), Postives = 221/473 (46.72%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +DFAIANN +P K    P L KQLC+       K ALMVLMI++K+AC   WFS+ +++E
Sbjct: 62  IDFAIANNDIPKKVEEFPWLLKQLCRHGTDVYTKTALMVLMISVKHACHLGWFSDSESQE 121

Query: 61  LYSLANEIGNDF--FGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAI 120
           L +LA+EI   F   G T+ G  +  +T + +MERF+P +KLG ++ S EVK GY + A 
Sbjct: 122 LIALADEIRTCFGSSGSTSPGIKSPGSTFSQIMERFYPFVKLGHVLVSFEVKAGYTMLAH 181

Query: 121 DFNILKTIQYAPQEKI---------------------------------------DTGPQ 180
           DF I K + ++ QEKI                                       DTGPQ
Sbjct: 182 DFYISKNMPHSLQEKIRLFVAQTDNIDTSACISNPPEVSFLLNGKGVEKRVNIAMDTGPQ 241

Query: 181 LPTNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDTGYI 240
           LPTN+T  LK G+NLLQ +G+F G+Y+I +A  G    P+  VL+D++Q       +G I
Sbjct: 242 LPTNVTAQLKYGTNLLQVMGNFKGNYIIIIAFTGLVVPPEKPVLKDYLQ-------SGVI 301

Query: 241 NLV--SERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQ--------- 300
                S+ + GP+ V++   +               RIK+PVKG+ CKHLQ         
Sbjct: 302 EASPDSDIIEGPSRVSLSCPISRK------------RIKLPVKGQLCKHLQCFDFSNYVH 361

Query: 301 ------------------------------VIREVAENVTEVIISADGSWKAILENDYGD 360
                                         ++++V  N  +VII A G+WK + +N    
Sbjct: 362 INMRNPTWRCPHCNQPVCYPDIRLDQNMAKILKDVEHNAADVIIDAGGTWK-VTKNTGET 421

Query: 361 GRPLDDSLNHQNERAQEESTAPPDVLDLTEVDDDMDI---CNLETEDRKPCLGNKNQPVS 389
             P+ + + H  E       + P V DLT  DDD ++    + + EDRKPC+        
Sbjct: 422 PEPVREII-HDLEDPMSLLNSGPVVFDLTG-DDDAELEVFGDNKVEDRKPCMS------- 481

BLAST of HG10009094 vs. ExPASy Swiss-Prot
Match: A0A0A7EPL0 (E4 SUMO-protein ligase PIAL1 OS=Arabidopsis thaliana OX=3702 GN=PIAL1 PE=2 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.9e-35
Identity = 210/852 (24.65%), Postives = 341/852 (40.02%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMK-HSPRLKAALMVLMITIKNACKAKWFSEKDAE 60
           +D AI  N VP     L  +   +C+ K    + +A +M LMI++K+AC+  WF E++ +
Sbjct: 31  IDAAIGRNEVPGNIQELALILNNVCRRKCDDYQTRAVVMALMISVKSACQLGWFPERETQ 90

Query: 61  ELYSLANEIGNDFFGDTNIGQ--SNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFA 120
           EL ++ + + N F    N+    ++ +T I+ V+ERF+P +KLG I+ S E KP   +  
Sbjct: 91  ELLAIIDLMWNGFSCPENVTSCVNSPVTLISQVIERFYPCVKLGHILVSFEAKPESKMMM 150

Query: 121 IDFNILKTIQYAPQEKI---------------------------------------DTGP 180
            DF+I K + ++P++K+                                       ++GP
Sbjct: 151 KDFHISKKMPHSPKQKVGLFVVRTEDISRSNCIVHPQGVSFLLNGKGIDKRVNISMESGP 210

Query: 181 QLPTNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQP--VLSTVDT 240
           QLPTN+T +L LG+NLLQAIG F G Y+IA+A M   P P+  +L+D++ P  V S  D 
Sbjct: 211 QLPTNVTALLNLGANLLQAIGCFGGSYLIAIAFMDVIPLPNKPLLKDYVHPEVVGSNSDC 270

Query: 241 GYINLVSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQ-------- 300
             I        GP+ +++   +              TRIK+PVKG  CKHLQ        
Sbjct: 271 DIIE-------GPSRISLSCPISR------------TRIKLPVKGHVCKHLQCFDFWNYV 330

Query: 301 -------------------------------VIREVAENVTEVIISADGSWKAILENDYG 360
                                          ++ EV  N  +V+ISADG+W    END  
Sbjct: 331 NMNTRRPSWRCPHCNQSVCYTDIRVDQKLRKILEEVGRNAADVVISADGTWMVETEND-- 390

Query: 361 DGRPLDDSLNHQNERAQEESTAPPDVLDLTEVDDDMDICNLETEDRKPCLGNKNQPVSST 420
           +   L     H +          P V +    +++M+  + + E+  PCL     P + T
Sbjct: 391 EDVELVPETTHDHGDPNSFINLGPTVKNPARDENEME-TSTQVEEHNPCLSEIQGPSNDT 450

Query: 421 LDISSGMNM--------NSLNQ---NLAAVLNDDFWSGIVTDGILTSSAGSDAPMGNS-T 480
              +S   M        N+L Q    L A     F +         S A    PM  S T
Sbjct: 451 HRPASDYTMLNQSHTSTNTLPQLPRTLNAFDGQQFVNLPQVINTRDSPASQALPMTFSPT 510

Query: 481 PPPGFAGIMQSTVLTDVVPPVLNHGAGVPG--HANFSSPAFYDQNNFQIQISN--SNENN 540
           P P      Q  + T+      N G  +P    + F         N + + S+  +  N+
Sbjct: 511 PSP------QDILATNAA----NFGTSMPAAQSSQFQGSHVTSLGNCEGRTSDLMARWNH 570

Query: 541 QYGRMTSIARPVSRTPIAVQALPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRDGLNTL 600
            YGR+ +   P    P++      Q+Q+         P   + PQ               
Sbjct: 571 IYGRVQTQFPP---APLSHHHYSMQNQSPSPAQQRPVPSYIAHPQT-------------- 630

Query: 601 SRDLERRQQFSRHHGDSHHATNL-APCHHSQTMQNRDPQDRSFTPGQSVQASTAVRPSTG 660
                    F  ++G++     + +   H QT+    P +      Q    S+   P T 
Sbjct: 631 ---------FHVNYGENADQRWMPSSIAHPQTL----PVNYGGNTNQRPIPSSIAHPQT- 690

Query: 661 LLTDFQNPHLQQALNLRISHLR---NQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAATP 720
           L  +++     ++    I+HL+   N   ++ +  +  S    Q     YGG A      
Sbjct: 691 LPVNYRGNTDHRSTPYSITHLQTLLNYGGNADQRPMPSSITNLQTLPATYGGYA------ 750

Query: 721 NSQHARMMAAS---QRTEMMRQSSAMSSQNQTSRSAHSLQTTPDGLRRPSGELRNVGVSQ 742
              H R M++S    RT  +        +   S   H  QT P      + ++ N G + 
Sbjct: 751 ---HQRPMSSSITHPRTSPVNYGGTPDQRPMPSSITHP-QTLPVSYGGTTDQILNPGGAM 806

BLAST of HG10009094 vs. ExPASy TrEMBL
Match: A0A1S3AUE1 (E4 SUMO-protein ligase PIAL2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482791 PE=4 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 607/833 (72.87%), Postives = 662/833 (79.47%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +D+AIANN+VPSKAH LP LFKQLCQ+KHS R KAALMVLM+TIKNACK +WFSEKDAEE
Sbjct: 47  IDYAIANNAVPSKAHSLPILFKQLCQLKHSHRSKAALMVLMLTIKNACKVRWFSEKDAEE 106

Query: 61  LYSLANEIGNDFFGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAIDF 120
           L  LANEIGNDFFGD NIGQ+NSLTTITTVMER+FP LKLGQIVAS+EVKPGYGV+A+DF
Sbjct: 107 LQRLANEIGNDFFGDMNIGQANSLTTITTVMERYFPCLKLGQIVASLEVKPGYGVYALDF 166

Query: 121 NILKTIQYAPQEKI---------------------------------------DTGPQLP 180
           NI +TIQ APQEK+                                       DTGPQLP
Sbjct: 167 NISRTIQCAPQEKLRLFVIQKDNTETSACMISPPQVNFLVNGRGVNGRINTHMDTGPQLP 226

Query: 181 TNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDTGYINL 240
           TNITHMLKLGSNLLQAIGSFNGHYV+AVAI GTAP+P+SSVLQDH+QPV+ST+D+     
Sbjct: 227 TNITHMLKLGSNLLQAIGSFNGHYVLAVAITGTAPSPNSSVLQDHVQPVVSTLDSD---- 286

Query: 241 VSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQ------------- 300
            S+ + GP+ ++            L   + YTRIK+PVKG SCKHLQ             
Sbjct: 287 -SDIIEGPSRIS------------LNCPISYTRIKIPVKGCSCKHLQCFDFDNFIDINSR 346

Query: 301 --------------------------VIREVAENVTEVIISADGSWKAILENDYGDGRPL 360
                                     VIREVAENVTEVIISADGSWKAILEND GDGR L
Sbjct: 347 RPSWRCPHCNQYICFLDIRVDQNMLKVIREVAENVTEVIISADGSWKAILENDNGDGRSL 406

Query: 361 DDSLNHQNERAQEESTAPPDVLDLTEVDDDMDICNLETEDRKPCLGNKNQPVSSTLDISS 420
           DDSLNHQ+ERAQEES+AP DVLDLTEV DDMDI + E EDRKPCLGNKNQPVSS+LD+SS
Sbjct: 407 DDSLNHQSERAQEESSAPSDVLDLTEVGDDMDIFDSEIEDRKPCLGNKNQPVSSSLDMSS 466

Query: 421 GMNMNSLNQNLAAVLNDDFWSGIVTDGILTSSAGSDAPMGNSTPPPGFAGIMQSTVLTDV 480
           GMNMNS +QNL+AV+ DDFWS +  DG+L SSAG DAPM NST PPGF  IMQS VLTDV
Sbjct: 467 GMNMNSFSQNLSAVVEDDFWSRL--DGVLISSAGLDAPMVNSTYPPGFTNIMQSAVLTDV 526

Query: 481 VPPVLNHGAGVPGHANFSSPAFYDQNNFQIQISNSNENNQYGRMTSIARPVSRTPIAVQA 540
           V PVLNHG GV GHANFSSPAFY+QNN  IQ+SNSNENNQYGR+TSI+ P SRTPIAVQA
Sbjct: 527 VQPVLNHGVGVLGHANFSSPAFYNQNNMHIQVSNSNENNQYGRVTSISIPASRTPIAVQA 586

Query: 541 LPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRDGLNTLSRDLERRQQFSRHHGDSHHAT 600
           LPAQS AAGQQYSSRTPII S+PQVGQSIPINRDGLN LSRDLERRQQFSRHHGDSHHAT
Sbjct: 587 LPAQSHAAGQQYSSRTPII-SSPQVGQSIPINRDGLNALSRDLERRQQFSRHHGDSHHAT 646

Query: 601 NLAPCHHSQTMQNRDPQDRSFTPGQSVQASTAVRPSTGLLTDFQNPHLQQALNLRISHLR 660
           NLA  HH QT+QNRDPQDRSFT GQS+Q S   RPSTGLLTDFQNPHLQQAL+ R+ HL+
Sbjct: 647 NLASFHHPQTVQNRDPQDRSFTTGQSIQTSNGARPSTGLLTDFQNPHLQQALS-RMPHLQ 706

Query: 661 NQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAATPNSQHARMMAASQRTEMMRQSSAMSS 720
           NQNSSSVRPSL FSRPMSQV GGGYGGS YT  TPNSQH+RMMAASQR EMMRQS +MS 
Sbjct: 707 NQNSSSVRPSLSFSRPMSQV-GGGYGGSTYTTVTPNSQHSRMMAASQRAEMMRQSPSMSL 766

Query: 721 QNQTSRSAHSLQTTPDGLRRPSGELRNV-GVSQSVTMAAGSVDLSVEQNWQPVGRMRGSL 755
           QNQTSRSAHSLQTTPDGLRRPSG+LRNV GVSQSVT+AAGSVDLS EQNWQP GRMRGSL
Sbjct: 767 QNQTSRSAHSLQTTPDGLRRPSGDLRNVGGVSQSVTIAAGSVDLSAEQNWQPAGRMRGSL 826

BLAST of HG10009094 vs. ExPASy TrEMBL
Match: A0A0A0KMH4 (SP-RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152190 PE=4 SV=1)

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 598/832 (71.88%), Postives = 656/832 (78.85%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +D+AIANN+VPSKAH LP+L KQLCQ+KHS R KAALMVLM+TIKNACK +WFSEKDAEE
Sbjct: 123 IDYAIANNAVPSKAHSLPSLVKQLCQLKHSHRSKAALMVLMLTIKNACKVRWFSEKDAEE 182

Query: 61  LYSLANEIGNDFFGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAIDF 120
           L  LANEIGNDFFGDTNIGQ+NSLTTITTVMER+FP LKLGQIVAS+EVKPGYGV+A+DF
Sbjct: 183 LQRLANEIGNDFFGDTNIGQANSLTTITTVMERYFPCLKLGQIVASLEVKPGYGVYALDF 242

Query: 121 NILKTIQYAPQEKI---------------------------------------DTGPQLP 180
           NI +T+QYA QEK+                                       DTGPQLP
Sbjct: 243 NISRTVQYASQEKLRLFVIQKDNTETSACIISPPQVNFLVNGRGINGRINTHMDTGPQLP 302

Query: 181 TNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDTGYINL 240
           TNITHMLKLGSNLLQA+GSFNGHYV+A+AI GTAP+PDSSVL DHIQP++ST+D+     
Sbjct: 303 TNITHMLKLGSNLLQAVGSFNGHYVLAIAITGTAPSPDSSVLHDHIQPIVSTLDSD---- 362

Query: 241 VSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQ------------- 300
            S+ + GP+ ++            L   + YTRIK+PVKG SCKHLQ             
Sbjct: 363 -SDIIEGPSRIS------------LNCPISYTRIKIPVKGCSCKHLQCFDFDNFIDINSR 422

Query: 301 --------------------------VIREVAENVTEVIISADGSWKAILENDYGDGRPL 360
                                     VIREVAENVTEVIIS DGSWKAILEND GDGR L
Sbjct: 423 RPSWRCPHCNQYICFLDIRVDRNMLKVIREVAENVTEVIISVDGSWKAILENDNGDGRSL 482

Query: 361 DDSLNHQNERAQEESTAPPDVLDLTEVDDDMDICNLETEDRKPCLGNKNQPVSSTLDISS 420
           +DSLNHQNERAQEES A PDVLD TEV DDMDI N E EDRKPCLGNKNQ VSS+LD+SS
Sbjct: 483 NDSLNHQNERAQEESAASPDVLDHTEVGDDMDIFNSEIEDRKPCLGNKNQRVSSSLDMSS 542

Query: 421 GMNMNSLNQNLAAVLNDDFWSGIVTDGILTSSAGSDAPMGNSTPPPGFAGIMQSTVLTDV 480
           GMNMNS +QNL+AV++DD WS I  DG+L S+AG DAPM NST PPGF G MQS VLTD 
Sbjct: 543 GMNMNSFSQNLSAVMDDDIWSRI--DGVLISTAGLDAPMVNSTYPPGFTGTMQSAVLTDA 602

Query: 481 VPPVLNHGAGVPGHANFSSPAFYDQNNFQIQISNSNENNQYGRMTSIARPVSRTPIAVQA 540
           V PVLNHG GV GHANF SPAFY+QNN QIQ+SNSNENNQYGR+TSI+RPVSRTP+AVQA
Sbjct: 603 VQPVLNHGVGVSGHANFPSPAFYNQNNVQIQVSNSNENNQYGRVTSISRPVSRTPVAVQA 662

Query: 541 LPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRDGLNTLSRDLERRQQFSRHHGDSHHAT 600
           LPAQS AAGQQYSSRTPII S+PQVGQSIPINRDGLN LSRDLERRQQFSRHHGDSHH+T
Sbjct: 663 LPAQSHAAGQQYSSRTPII-SSPQVGQSIPINRDGLNALSRDLERRQQFSRHHGDSHHST 722

Query: 601 NLAPCHHSQTMQNRDPQDRSFTPGQSVQASTAVRPSTGLLTDFQNPHLQQALNLRISHLR 660
           NLA  HH QT+QNRDPQDRSFT GQS+Q S+  RPS GLL DFQNPHLQQALN+R+ HL+
Sbjct: 723 NLASFHHPQTVQNRDPQDRSFTTGQSIQTSSGARPSPGLLADFQNPHLQQALNMRMPHLQ 782

Query: 661 NQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAATPNSQHARMMAASQRTEMMRQSSAMSS 720
           NQNSSSVR SL FSRPMSQV GGGYGGS YT  TPNSQHARM+AASQR EMMRQS  MS 
Sbjct: 783 NQNSSSVRTSLSFSRPMSQV-GGGYGGSTYTTVTPNSQHARMLAASQRVEMMRQSPPMSL 842

Query: 721 QNQTSRSAHSLQTTPDGLRRPSGELRNVGVSQSVTMAAGSVDLSVEQNWQPVGRMRGSLS 755
            NQTSRSAHSLQTTPDGLRRPSG+LRNVGVSQSVTMAAGSVDLS EQNWQP GRMRGSLS
Sbjct: 843 HNQTSRSAHSLQTTPDGLRRPSGDLRNVGVSQSVTMAAGSVDLSAEQNWQPAGRMRGSLS 902

BLAST of HG10009094 vs. ExPASy TrEMBL
Match: A0A6J1ELU7 (E4 SUMO-protein ligase PIAL2-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111435722 PE=4 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 590/799 (73.84%), Postives = 643/799 (80.48%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +DFAIAN+ VPSKA GLP+L KQ+CQ KHS  LKAA+MVLMI  KNACK KWFSEK+AEE
Sbjct: 47  IDFAIANDFVPSKAQGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEE 106

Query: 61  LYSLANEIGNDFFGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAIDF 120
           LYSLANEIG+DFF DTN G SNSLTTITTVMERFFPRLKLGQIV S EVKPGYGVFA DF
Sbjct: 107 LYSLANEIGSDFFVDTNTGPSNSLTTITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDF 166

Query: 121 NILKTIQYAPQEKI--------------------------------------------DT 180
           NI KTIQYAPQEKI                                            DT
Sbjct: 167 NISKTIQYAPQEKIRLFVAQKDNTETSACIISPPQLPCQWEGSQWKDKYIHASFLFNKDT 226

Query: 181 GPQLPTNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDT 240
           GPQLPTN+THMLKLGSNLLQ IGSFNGHYVIAVA+MG+AP+PDSSVLQDH QPV+STVD+
Sbjct: 227 GPQLPTNVTHMLKLGSNLLQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPVVSTVDS 286

Query: 241 GYINLVSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQVIREVAEN 300
                 S+ + GP+ ++            L   + YTRIKVPVKGRSCKHLQVIREVAEN
Sbjct: 287 D-----SDIIEGPSRIS------------LNCPISYTRIKVPVKGRSCKHLQVIREVAEN 346

Query: 301 VTEVIISADGSWKAILENDYGDGRPLDDSLNHQNERAQEESTAPPDVLDLTEVDDDMDIC 360
           VTEVIISADGSWKAILEND GDGRPLDDSLN QNERAQ+ESTAPPDVLDLTEVDDDM+IC
Sbjct: 347 VTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDDDMNIC 406

Query: 361 NLETEDRKPCLGNKNQPVSSTLDISSGMNMNSLNQNLAAVLNDDFWSGIVTDGILTSSAG 420
           NLETEDRKPCLGNKNQPVSS+L+I SGMN NSLNQN +A L+DDFWSG+VTD +L SS  
Sbjct: 407 NLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSGMVTDRLLISSIR 466

Query: 421 SDAPMGNSTPPPGFAGIMQSTVLTDVVPPVLNHGAGVPGHANFSSPAFYDQNNFQIQISN 480
           SDAPMG+ST  P FAG+ QS  LTD V PVLNH  GVPG  NF  PAFYDQNN Q+Q+SN
Sbjct: 467 SDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPAFYDQNNVQVQVSN 526

Query: 481 SNENNQYGRMTSIARPVSRTPIAVQALPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRD 540
           SNE+NQYGRMTSIARPVSRT +A Q LPAQSQ +GQQYSSRT  I SAPQVGQSIPI+RD
Sbjct: 527 SNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTISSAPQVGQSIPISRD 586

Query: 541 GLNTLSRDLERRQQFSRHHGDSHHATNLAPCHHSQTMQNRDPQDRSFTPGQSVQASTAVR 600
           GLNT+SRD ERRQ F RHHGD HHATNLAP      +QNR+PQDRSFTPGQSV+ASTA R
Sbjct: 587 GLNTISRDSERRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQSVRASTAQR 646

Query: 601 PSTGLLTDFQNPHLQQALNLRISHLRNQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAAT 660
           PS G+LTDFQNPHLQQ+LNLRISHLRNQN SSVRPSL FSRP SQV GGGYGGSAY A T
Sbjct: 647 PSAGILTDFQNPHLQQSLNLRISHLRNQNPSSVRPSLPFSRPTSQV-GGGYGGSAYPAVT 706

Query: 661 PNSQHARMMAASQRTEMMRQSSAMSSQNQTSRSAHSLQTTPDGLRRPSGELRNV-GVSQS 720
           P++QHARMM ASQR EMMRQSSAMS QNQTSRS H LQTTPDGLRRP+G+LRNV G++QS
Sbjct: 707 PHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLRRPAGDLRNVGGMTQS 766

Query: 721 VTMAAGSVDLSVEQNWQPVGRMRGSLSGRAYSDAYGVIIQPTQAAQSARPPSHLTPTQPS 755
           VTMA+  +D SVEQN QP+GRMRGSLSGRAYSDAYGVIIQPTQ  QS RPPS+LT TQ +
Sbjct: 767 VTMASDLLDPSVEQNRQPIGRMRGSLSGRAYSDAYGVIIQPTQPVQSTRPPSNLTTTQSN 826

BLAST of HG10009094 vs. ExPASy TrEMBL
Match: A0A6J1I477 (uncharacterized protein LOC111469015 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111469015 PE=4 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 587/799 (73.47%), Postives = 642/799 (80.35%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +DFAIAN+ VPSKA GLP+L KQ+CQ KHS  LKAA+MVLMI  KNACK KWFSEK+AEE
Sbjct: 47  IDFAIANDFVPSKAQGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEE 106

Query: 61  LYSLANEIGNDFFGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAIDF 120
           LYSLANEIG+DFF DTN G SNSLTTIT VMERFFPRLKLGQIV S EVKPGYGVFA DF
Sbjct: 107 LYSLANEIGSDFFVDTNTGPSNSLTTITKVMERFFPRLKLGQIVISAEVKPGYGVFAFDF 166

Query: 121 NILKTIQYAPQEKI--------------------------------------------DT 180
           NI KTIQYAPQEKI                                            DT
Sbjct: 167 NISKTIQYAPQEKIRLFVAQKDNTETSACIISPPQLPCQWEGSQWKDKYIHASFLFNKDT 226

Query: 181 GPQLPTNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDT 240
           GPQLPTN+THMLKLGSNLLQ IGSFNGHYVI+VA+MG+AP+PDSSVLQDH QP +STVD+
Sbjct: 227 GPQLPTNVTHMLKLGSNLLQVIGSFNGHYVISVAVMGSAPSPDSSVLQDHEQPAVSTVDS 286

Query: 241 GYINLVSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQVIREVAEN 300
                 S+ + GP+ ++            L   + YTRIKVPVKGRSCKHLQVIREVAEN
Sbjct: 287 D-----SDIIEGPSRIS------------LNCPISYTRIKVPVKGRSCKHLQVIREVAEN 346

Query: 301 VTEVIISADGSWKAILENDYGDGRPLDDSLNHQNERAQEESTAPPDVLDLTEVDDDMDIC 360
           VTEVIISADGSWKAILEND GDGRPLDDSLN QNERAQ+ESTAPPDVLDLTEVDDDM+IC
Sbjct: 347 VTEVIISADGSWKAILENDCGDGRPLDDSLNQQNERAQQESTAPPDVLDLTEVDDDMNIC 406

Query: 361 NLETEDRKPCLGNKNQPVSSTLDISSGMNMNSLNQNLAAVLNDDFWSGIVTDGILTSSAG 420
           NLETEDRKPCLGNKNQPVSS+L+I SGMN NSLNQN +A L+DDFWS +VTD +LTSS  
Sbjct: 407 NLETEDRKPCLGNKNQPVSSSLNILSGMNRNSLNQNFSAALDDDFWSRMVTDRLLTSSIR 466

Query: 421 SDAPMGNSTPPPGFAGIMQSTVLTDVVPPVLNHGAGVPGHANFSSPAFYDQNNFQIQISN 480
           SDAPMG+ST  P FAG+ QS  LTD V PVLNH  GVPG  NF  P+FYDQNN Q+Q+SN
Sbjct: 467 SDAPMGSSTAAPSFAGLTQSAGLTDAVSPVLNHDVGVPGQVNFPFPSFYDQNNVQVQVSN 526

Query: 481 SNENNQYGRMTSIARPVSRTPIAVQALPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRD 540
           SNE+NQYGRMTSIARPVSRT +A Q LPAQSQ +GQQYSSRT  + SAPQVGQSIPI+RD
Sbjct: 527 SNESNQYGRMTSIARPVSRT-LAGQVLPAQSQTSGQQYSSRTSTVSSAPQVGQSIPISRD 586

Query: 541 GLNTLSRDLERRQQFSRHHGDSHHATNLAPCHHSQTMQNRDPQDRSFTPGQSVQASTAVR 600
           GLNT+SRD E RQ F RHHGD HHATNLAP      +QNR+PQDRSFTPGQSV+ASTA R
Sbjct: 587 GLNTISRDSEMRQPFPRHHGDLHHATNLAPFLRPPIVQNREPQDRSFTPGQSVRASTAQR 646

Query: 601 PSTGLLTDFQNPHLQQALNLRISHLRNQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAAT 660
           PS G+LTDFQNPHLQQALNLRISHL+NQN SSVRPSL FSRP SQV GGGYGGSAYTA T
Sbjct: 647 PSVGILTDFQNPHLQQALNLRISHLQNQNPSSVRPSLPFSRPTSQV-GGGYGGSAYTAVT 706

Query: 661 PNSQHARMMAASQRTEMMRQSSAMSSQNQTSRSAHSLQTTPDGLRRPSGELRNV-GVSQS 720
           P++QHARMM ASQR EMMRQSSAMS QNQTSRS H LQTTPDGLRRP+GELRNV G++QS
Sbjct: 707 PHNQHARMMVASQRAEMMRQSSAMSLQNQTSRSPHPLQTTPDGLRRPAGELRNVGGMTQS 766

Query: 721 VTMAAGSVDLSVEQNWQPVGRMRGSLSGRAYSDAYGVIIQPTQAAQSARPPSHLTPTQPS 755
           VTMA+  +D SVEQN QP+GRMRGSLSGRAYSDA+GVIIQPTQ  QSARPPS+LT TQ S
Sbjct: 767 VTMASNLLDPSVEQNRQPIGRMRGSLSGRAYSDAFGVIIQPTQPVQSARPPSNLTTTQSS 826

BLAST of HG10009094 vs. ExPASy TrEMBL
Match: A0A6J1ESZ6 (E4 SUMO-protein ligase PIAL2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435722 PE=4 SV=1)

HSP 1 Score: 1079.7 bits (2791), Expect = 0.0e+00
Identity = 590/833 (70.83%), Postives = 643/833 (77.19%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +DFAIAN+ VPSKA GLP+L KQ+CQ KHS  LKAA+MVLMI  KNACK KWFSEK+AEE
Sbjct: 47  IDFAIANDFVPSKAQGLPSLLKQICQKKHSHHLKAAIMVLMIAAKNACKVKWFSEKEAEE 106

Query: 61  LYSLANEIGNDFFGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAIDF 120
           LYSLANEIG+DFF DTN G SNSLTTITTVMERFFPRLKLGQIV S EVKPGYGVFA DF
Sbjct: 107 LYSLANEIGSDFFVDTNTGPSNSLTTITTVMERFFPRLKLGQIVISAEVKPGYGVFAFDF 166

Query: 121 NILKTIQYAPQEKI---------------------------------------DTGPQLP 180
           NI KTIQYAPQEKI                                       DTGPQLP
Sbjct: 167 NISKTIQYAPQEKIRLFVAQKDNTETSACIISPPQVNFLVNGRGVNGRTNIYMDTGPQLP 226

Query: 181 TNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDTGYINL 240
           TN+THMLKLGSNLLQ IGSFNGHYVIAVA+MG+AP+PDSSVLQDH QPV+STVD+     
Sbjct: 227 TNVTHMLKLGSNLLQVIGSFNGHYVIAVAVMGSAPSPDSSVLQDHEQPVVSTVDSD---- 286

Query: 241 VSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQ------------- 300
            S+ + GP+ ++            L   + YTRIKVPVKGRSCKHLQ             
Sbjct: 287 -SDIIEGPSRIS------------LNCPISYTRIKVPVKGRSCKHLQCFDFYNFIDINSR 346

Query: 301 --------------------------VIREVAENVTEVIISADGSWKAILENDYGDGRPL 360
                                     VIREVAENVTEVIISADGSWKAILEND GDGRPL
Sbjct: 347 RPSWRCPHCNQYICFLDICIDQNMLKVIREVAENVTEVIISADGSWKAILENDCGDGRPL 406

Query: 361 DDSLNHQNERAQEESTAPPDVLDLTEVDDDMDICNLETEDRKPCLGNKNQPVSSTLDISS 420
           DDSLN QNERAQ+ESTAPPDVLDLTEVDDDM+ICNLETEDRKPCLGNKNQPVSS+L+I S
Sbjct: 407 DDSLNQQNERAQQESTAPPDVLDLTEVDDDMNICNLETEDRKPCLGNKNQPVSSSLNILS 466

Query: 421 GMNMNSLNQNLAAVLNDDFWSGIVTDGILTSSAGSDAPMGNSTPPPGFAGIMQSTVLTDV 480
           GMN NSLNQN +A L+DDFWSG+VTD +L SS  SDAPMG+ST  P FAG+ QS  LTD 
Sbjct: 467 GMNRNSLNQNFSAALDDDFWSGMVTDRLLISSIRSDAPMGSSTAAPSFAGLTQSAGLTDA 526

Query: 481 VPPVLNHGAGVPGHANFSSPAFYDQNNFQIQISNSNENNQYGRMTSIARPVSRTPIAVQA 540
           V PVLNH  GVPG  NF  PAFYDQNN Q+Q+SNSNE+NQYGRMTSIARPVSRT +A Q 
Sbjct: 527 VSPVLNHDVGVPGQVNFPFPAFYDQNNVQVQVSNSNESNQYGRMTSIARPVSRT-LAGQV 586

Query: 541 LPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRDGLNTLSRDLERRQQFSRHHGDSHHAT 600
           LPAQSQ +GQQYSSRT  I SAPQVGQSIPI+RDGLNT+SRD ERRQ F RHHGD HHAT
Sbjct: 587 LPAQSQTSGQQYSSRTSTISSAPQVGQSIPISRDGLNTISRDSERRQPFPRHHGDLHHAT 646

Query: 601 NLAPCHHSQTMQNRDPQDRSFTPGQSVQASTAVRPSTGLLTDFQNPHLQQALNLRISHLR 660
           NLAP      +QNR+PQDRSFTPGQSV+ASTA RPS G+LTDFQNPHLQQ+LNLRISHLR
Sbjct: 647 NLAPFLRPPIVQNREPQDRSFTPGQSVRASTAQRPSAGILTDFQNPHLQQSLNLRISHLR 706

Query: 661 NQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAATPNSQHARMMAASQRTEMMRQSSAMSS 720
           NQN SSVRPSL FSRP SQV GGGYGGSAY A TP++QHARMM ASQR EMMRQSSAMS 
Sbjct: 707 NQNPSSVRPSLPFSRPTSQV-GGGYGGSAYPAVTPHNQHARMMVASQRAEMMRQSSAMSL 766

Query: 721 QNQTSRSAHSLQTTPDGLRRPSGELRNV-GVSQSVTMAAGSVDLSVEQNWQPVGRMRGSL 755
           QNQTSRS H LQTTPDGLRRP+G+LRNV G++QSVTMA+  +D SVEQN QP+GRMRGSL
Sbjct: 767 QNQTSRSPHPLQTTPDGLRRPAGDLRNVGGMTQSVTMASDLLDPSVEQNRQPIGRMRGSL 826

BLAST of HG10009094 vs. TAIR 10
Match: AT5G41580.1 (RING/U-box superfamily protein )

HSP 1 Score: 197.2 bits (500), Expect = 4.7e-50
Identity = 149/473 (31.50%), Postives = 221/473 (46.72%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMKHSPRLKAALMVLMITIKNACKAKWFSEKDAEE 60
           +DFAIANN +P K    P L KQLC+       K ALMVLMI++K+AC   WFS+ +++E
Sbjct: 62  IDFAIANNDIPKKVEEFPWLLKQLCRHGTDVYTKTALMVLMISVKHACHLGWFSDSESQE 121

Query: 61  LYSLANEIGNDF--FGDTNIGQSNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFAI 120
           L +LA+EI   F   G T+ G  +  +T + +MERF+P +KLG ++ S EVK GY + A 
Sbjct: 122 LIALADEIRTCFGSSGSTSPGIKSPGSTFSQIMERFYPFVKLGHVLVSFEVKAGYTMLAH 181

Query: 121 DFNILKTIQYAPQEKI---------------------------------------DTGPQ 180
           DF I K + ++ QEKI                                       DTGPQ
Sbjct: 182 DFYISKNMPHSLQEKIRLFVAQTDNIDTSACISNPPEVSFLLNGKGVEKRVNIAMDTGPQ 241

Query: 181 LPTNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQPVLSTVDTGYI 240
           LPTN+T  LK G+NLLQ +G+F G+Y+I +A  G    P+  VL+D++Q       +G I
Sbjct: 242 LPTNVTAQLKYGTNLLQVMGNFKGNYIIIIAFTGLVVPPEKPVLKDYLQ-------SGVI 301

Query: 241 NLV--SERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQ--------- 300
                S+ + GP+ V++   +               RIK+PVKG+ CKHLQ         
Sbjct: 302 EASPDSDIIEGPSRVSLSCPISRK------------RIKLPVKGQLCKHLQCFDFSNYVH 361

Query: 301 ------------------------------VIREVAENVTEVIISADGSWKAILENDYGD 360
                                         ++++V  N  +VII A G+WK + +N    
Sbjct: 362 INMRNPTWRCPHCNQPVCYPDIRLDQNMAKILKDVEHNAADVIIDAGGTWK-VTKNTGET 421

Query: 361 GRPLDDSLNHQNERAQEESTAPPDVLDLTEVDDDMDI---CNLETEDRKPCLGNKNQPVS 389
             P+ + + H  E       + P V DLT  DDD ++    + + EDRKPC+        
Sbjct: 422 PEPVREII-HDLEDPMSLLNSGPVVFDLTG-DDDAELEVFGDNKVEDRKPCMS------- 481

BLAST of HG10009094 vs. TAIR 10
Match: AT1G08910.1 (zinc ion binding;zinc ion binding )

HSP 1 Score: 159.5 bits (402), Expect = 1.1e-38
Identity = 210/834 (25.18%), Postives = 341/834 (40.89%), Query Frame = 0

Query: 1   MDFAIANNSVPSKAHGLPTLFKQLCQMK-HSPRLKAALMVLMITIKNACKAKWFSEKDAE 60
           +D AI  N VP     L  +   +C+ K    + +A +M LMI++K+AC+  WF E++ +
Sbjct: 31  IDAAIGRNEVPGNIQELALILNNVCRRKCDDYQTRAVVMALMISVKSACQLGWFPERETQ 90

Query: 61  ELYSLANEIGNDFFGDTNIGQ--SNSLTTITTVMERFFPRLKLGQIVASVEVKPGYGVFA 120
           EL ++ + + N F    N+    ++ +T I+ V+ERF+P +KLG I+ S E KP   +  
Sbjct: 91  ELLAIIDLMWNGFSCPENVTSCVNSPVTLISQVIERFYPCVKLGHILVSFEAKPESKMMM 150

Query: 121 IDFNILKTIQYAPQEKI---------------------------------------DTGP 180
            DF+I K + ++P++K+                                       ++GP
Sbjct: 151 KDFHISKKMPHSPKQKVGLFVVRTEDISRSNCIVHPQGVSFLLNGKGIDKRVNISMESGP 210

Query: 181 QLPTNITHMLKLGSNLLQAIGSFNGHYVIAVAIMGTAPAPDSSVLQDHIQP--VLSTVDT 240
           QLPTN+T +L LG+NLLQAIG F G Y+IA+A M   P P+  +L+D++ P  V S  D 
Sbjct: 211 QLPTNVTALLNLGANLLQAIGCFGGSYLIAIAFMDVIPLPNKPLLKDYVHPEVVGSNSDC 270

Query: 241 GYINLVSERMPGPTLVAIGRRLRNSWQRRLQEGVFYTRIKVPVKGRSCKHLQ-------- 300
             I        GP+ +++   +              TRIK+PVKG  CKHLQ        
Sbjct: 271 DIIE-------GPSRISLSCPISR------------TRIKLPVKGHVCKHLQCFDFWNYV 330

Query: 301 -------------VIREVAENVTEVIISADGSWKAILENDYGDGRPLDDSLNHQNERAQE 360
                        ++ EV  N  +V+ISADG+W    END  +   L     H +     
Sbjct: 331 NMNTRRHHGAARIILEEVGRNAADVVISADGTWMVETEND--EDVELVPETTHDHGDPNS 390

Query: 361 ESTAPPDVLDLTEVDDDMDICNLETEDRKPCLGNKNQPVSSTLDISSGMNM--------N 420
                P V +    +++M+  + + E+  PCL     P + T   +S   M        N
Sbjct: 391 FINLGPTVKNPARDENEME-TSTQVEEHNPCLSEIQGPSNDTHRPASDYTMLNQSHTSTN 450

Query: 421 SLNQ---NLAAVLNDDFWSGIVTDGILTSSAGSDAPMGNS-TPPPGFAGIMQSTVLTDVV 480
           +L Q    L A     F +         S A    PM  S TP P      Q  + T+  
Sbjct: 451 TLPQLPRTLNAFDGQQFVNLPQVINTRDSPASQALPMTFSPTPSP------QDILATNAA 510

Query: 481 PPVLNHGAGVPG--HANFSSPAFYDQNNFQIQISN--SNENNQYGRMTSIARPVSRTPIA 540
               N G  +P    + F         N + + S+  +  N+ YGR+ +   P    P++
Sbjct: 511 ----NFGTSMPAAQSSQFQGSHVTSLGNCEGRTSDLMARWNHIYGRVQTQFPP---APLS 570

Query: 541 VQALPAQSQAAGQQYSSRTPIIFSAPQVGQSIPINRDGLNTLSRDLERRQQFSRHHGDSH 600
                 Q+Q+         P   + PQ                        F  ++G++ 
Sbjct: 571 HHHYSMQNQSPSPAQQRPVPSYIAHPQT-----------------------FHVNYGENA 630

Query: 601 HATNL-APCHHSQTMQNRDPQDRSFTPGQSVQASTAVRPSTGLLTDFQNPHLQQALNLRI 660
               + +   H QT+    P +      Q    S+   P T L  +++     ++    I
Sbjct: 631 DQRWMPSSIAHPQTL----PVNYGGNTNQRPIPSSIAHPQT-LPVNYRGNTDHRSTPYSI 690

Query: 661 SHLR---NQNSSSVRPSLQFSRPMSQVGGGGYGGSAYTAATPNSQHARMMAAS---QRTE 720
           +HL+   N   ++ +  +  S    Q     YGG A         H R M++S    RT 
Sbjct: 691 THLQTLLNYGGNADQRPMPSSITNLQTLPATYGGYA---------HQRPMSSSITHPRTS 750

Query: 721 MMRQSSAMSSQNQTSRSAHSLQTTPDGLRRPSGELRNVGVSQSVTMAAGSVDLSV--EQN 742
            +        +   S   H  QT P      + ++ N G +     +   ++L+    +N
Sbjct: 751 PVNYGGTPDQRPMPSSITHP-QTLPVSYGGTTDQILNPGGAMGQFSSREFMNLTPANTEN 788