HG10014729 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10014729
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	tRNA (guanine(26)-N(2))-dimethyltransferase
Location	Chr02: 18792803 .. 18796705 (+)
RNA-Seq Expression	HG10014729
Synteny	HG10014729

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAACGGTGGCGCCGAGAACCCTATCTTCTTCCCCGTTTCTTCACAGCCCACAAACAAATTCCCATTCCTTCTTCTCTTCAACAATTCATCTCTTCCAATTTTCACACTCCAACGTAAAATCCCTCAACAATTGCACCGAAAGGGGCATAGAATTTGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTCTACCGTAAATCCAAGGGCCATCTTCGGGTTCTTGACGCATTGTGCGGTTGCGGCATCCGGTCGCTTCGGTACTTGGTCGAGGCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGACGATAATCGGAGGGTTATTGTGAAGAATCTATCGACGGTTGCGGAAGATTCTGGAGATGCTCGGCGGTGGGTGGTTACTAATTGTGATGCGAACAGGGTTATGACGGAGTTCTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCATCGTTTTTGAGGCCCGCTATAAATGCGTTGAAATTGGATGGATTGCTCTATGTTACGTCCACAGATGGCTTCTCTTCCGGCGGTCATCGGCCTTCTCAGTGAGTGCAATCTGTTATACTTTGTACTCTGTTAATTATTTGCTTTATCCGCTTCATTGAATTTCTTGATATGAAATGTCTGATCATTTTAACGGCTTGTATCTATATATAAAAGATTAAACATAGCATTATTTTAGTTTTGAGTTTTAGTAGTTTAATATGGTGTTAGCTCTCAATTAATATTGCTAATCACTTGTTGAGTTGTTGAGTCTTTGTATTTAGGACTAAGCAATTCGACTTGGAATTGACAGATTGGTTTAACGAAAACTTTAAACTGACTTTAAAGCTGAATTTCTTAAAGATTTCGGATCAATCTATATTGATTGACTGTCTCCATTATGTTGTTGCTAGCAAATACCACTATTTTTTGTTTTGGATCTTCCAAATCATTCCTAGGGGAGGTTCGGACCCATTTTTTTCTGATCCTTCGATAAAAAGATTCATTAGTTAGTTATATATATCGCCTCCTTTATTACAGTTCTATGGGGGATATCACATGTCGTACTCTATCAAAATTTTAGATTTTCTATTTAATGATAATGAAAAAATAAATTGTAAAAATGGAATTCCGACCATTTCCTATAAACATCTTATATAGATAAGATACCCAATTAGATTTAGATAATAGTAATCGTTTATGTTCGGGGGTTTACATATACTCATAATCGCTATTATAATTTTAATTAAGAAGGATTTTTTATGGTTATGGAAACGAATCAATACGGATGGATTAGTAATGATTAGTTATGTATCAATTTTGATTTTTTTAGGTAAGGTATCAATTTTGGGATTGTTTTCTTATATCATTAGCTAAACTGACAAACCAGCCGAAATAGAATAAGGGTTACTAAAAATAACAAAATTTATCCTAATCATTCTGACTGTTTTTTGGTTTTGACTTGCACCCTTACTTGTGCTAAGTTTTAAGTCTAAAGGTAAAATGGTTGCTAAGTAACTGAATATAGGTGGGCAATTATATGAGATGTGGTAATTTAAATTCTTTTTTTCTTTTTCTTGTTTCTCTCTTTTTTTTCATTCCATCTCTCTAGATCTTTAGCTGCATATGGAGCATATGTGCGTCCCATGCCGTTTTCGAATGAAATTGGTTTGCGAATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACGCCACTCTTTTCATATTATTCATACCATGGACCTGTTTTTAGAGTTATGTTACGAATCAATCGAGGGAGGATTTATAAAAACAGGTGATAGTTGATGAGTTTAGCATGTCTGCTAGTGCTGGATGTATCTTGTATGATCATTGATGGTGATTGATTCAAGCTCTCACTATTTATATCAGGCATCATGGTTTCGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGTTGTCCTTGCAATGATTCAAGGGTAAGACTATGACTACTATGACTGTCATTCTGCTACCCTTTTGAGCTGAAGCATCCACTGGTTTCAGCTTCCACATTTCAAACTGATTCCAAAACTTTCTCATTTGGTTTGCATGGTCCAGATAACCAGTGGCTTCAAGCTGAATATGCTTGAATTTATTGTTTTTTTATTCATTGGTTCTTGCTTTCTAATGTAATTGTTAAAGTGTTGATATAATGTTACATTTACCTCCACCCAACAGCTTAAGCTTTTGGGTCAATAGATGATTTAACATCCTATTAGAATAGGAGGTCTAGAAGGTTATGTGTTCAAACCCCTATAATATTATTTCCTCCTCAATTAATATTAATTAATATTGATTTTCACTTGTTAGACCTTCTACAAATTTCCAAGGCCACAAGTGAGAGGGAGCATTAAAGTATTGATATAATATTAAATTTACCTTCACCCATCAACTTAAGTCTTTGGTCAATCGGTGATTTAACAGTAATTACTTTCAACATTCAATCTTGTTTGTTCGAGATACATTAAAAATTTTGACTTGTTAGGTTTCTGAGTCACTTGTGGTTTCGGGACCTCTTTGGACTGGACCTCTACACAGTGCAGACTACATTGAAGACATGTTAACCTTGGCTAAGCAGTGGGAATGGATAGGCAATGGTCAGGGGAAGGATCTTGAGAAACTTCTGGGGCAGATGGTGGATGAGAGTGACTCCAAACTGCCAGTAGGGTACATCAAAATGGACGAGGTGGCCAAATTTAACTTATTCTTCTTATGGTTTAATATTTCATTTCTGTTTTTGGTCTAACAATGAAAGAATTGATCTAACAAGGCCCCCTTCTCTATGCTTAGGTGGCAAGTCGAGCAAAAGTAAATTCACCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGTGAGAATATTGGTAATTAAGATGTGATAAATGGTGTGACGCTTGTTATTGTCCACACGGCTGAACTGTTTTTGTTGTCATGAGATTCTACTAAATGCTAGAAAGCAAAGAAATATTGGCACAGGCATTAGACACAAATACAACAGGACACAAATACAATGATCGGTTATTCTCTATGAGTAGGACATAGACACGGCATGGATATGTAATATGTTTAGTATAATGTATATCTTTTTAAACTATATCGTTTTTATACAATTTTGTAGCTTGTAAGCTTTATGTATTCATCTGTTTTGAGAAAAATGAGTTTGGATATGTTTTGCTTTCAAATTTATTATTATTATCGTCAAAGTAATCTTGGCTCAGTGGTATTGGCATGCATTCCGCCCCTAGAGGTTGGAGGTTCAATCCCCCCACCCCTTGTTGTACTCAAAAAAAAAAAGAAGAAAAAAAGAAAGAAAGAAATTATTATTATTGCAATCTTACCATACATATCTATTTATTAAGCTTAACAAGTATCTGACAGTGTCTAATAAGTGTTAAAACTTATATTCAATTTGTTCAATTAGTGTCTAAACACACATCTACTTGTCTATTGTGCAAAACAAGTATACCACGTGTGTTTGACATGTTTTGGAGTGCCCAATACGTGTTGAACATGGACAAGTTATCCAAACTAGAATGTTTGTGTTTCATAACTGGAAAGTGAACAGGTTTTGGCTTGGAATGTAGATGCGTGAAAAGAAAAATAGTTCTCGCTTTTCTTTCTCTTTCTGAAGGATTTTGGTCGTTGCAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAATGCAATCAAGACAAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCTTGAACTGCGGCAGAATTGTCTTGGTGTGCATCAAGTCACATGA

mRNA sequence

ATGTTAACGGTGGCGCCGAGAACCCTATCTTCTTCCCCGTTTCTTCACAGCCCACAAACAAATTCCCATTCCTTCTTCTCTTCAACAATTCATCTCTTCCAATTTTCACACTCCAACGTAAAATCCCTCAACAATTGCACCGAAAGGGGCATAGAATTTGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTCTACCGTAAATCCAAGGGCCATCTTCGGGTTCTTGACGCATTGTGCGGTTGCGGCATCCGGTCGCTTCGGTACTTGGTCGAGGCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGACGATAATCGGAGGGTTATTGTGAAGAATCTATCGACGGTTGCGGAAGATTCTGGAGATGCTCGGCGGTGGGTGGTTACTAATTGTGATGCGAACAGGGTTATGACGGAGTTCTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCATCGTTTTTGAGGCCCGCTATAAATGCGTTGAAATTGGATGGATTGCTCTATGTTACGTCCACAGATGGCTTCTCTTCCGGCGGTCATCGGCCTTCTCAATCTTTAGCTGCATATGGAGCATATGTGCGTCCCATGCCGTTTTCGAATGAAATTGGTTTGCGAATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACGCCACTCTTTTCATATTATTCATACCATGGACCTGTTTTTAGAGTTATGTTACGAATCAATCGAGGGAGGATTTATAAAAACAGGCATCATGGTTTCGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGTTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTTGTGGTTTCGGGACCTCTTTGGACTGGACCTCTACACAGTGCAGACTACATTGAAGACATGTTAACCTTGGCTAAGCAGTGGGAATGGATAGGCAATGGTCAGGGGAAGGATCTTGAGAAACTTCTGGGGCAGATGGTGGATGAGAGTGACTCCAAACTGCCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGAGCAAAAGTAAATTCACCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAATGCAATCAAGACAAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCTTGAACTGCGGCAGAATTGTCTTGGTGTGCATCAAGTCACATGA

Coding sequence (CDS)

ATGTTAACGGTGGCGCCGAGAACCCTATCTTCTTCCCCGTTTCTTCACAGCCCACAAACAAATTCCCATTCCTTCTTCTCTTCAACAATTCATCTCTTCCAATTTTCACACTCCAACGTAAAATCCCTCAACAATTGCACCGAAAGGGGCATAGAATTTGACATCGGAGACACCTTCTTCCGCCATGAAAGCGCCACCGGACGCGACTTGGGCGTCCTCGCCGCCGCGCTCTACCGTAAATCCAAGGGCCATCTTCGGGTTCTTGACGCATTGTGCGGTTGCGGCATCCGGTCGCTTCGGTACTTGGTCGAGGCCGAGGCTGATTTTGTGTTGGCGAATGACGCTAATGACGATAATCGGAGGGTTATTGTGAAGAATCTATCGACGGTTGCGGAAGATTCTGGAGATGCTCGGCGGTGGGTGGTTACTAATTGTGATGCGAACAGGGTTATGACGGAGTTCTACTTGAAGAAGGATTTCTTTGATTTTATTGATGTTGATTCCTTTGGGAGTGATTCATCGTTTTTGAGGCCCGCTATAAATGCGTTGAAATTGGATGGATTGCTCTATGTTACGTCCACAGATGGCTTCTCTTCCGGCGGTCATCGGCCTTCTCAATCTTTAGCTGCATATGGAGCATATGTGCGTCCCATGCCGTTTTCGAATGAAATTGGTTTGCGAATGCTTATAGGTGGGGTTGCACGTGAAGCTTCTGTACTGGGTTACTATGCTACGCCACTCTTTTCATATTATTCATACCATGGACCTGTTTTTAGAGTTATGTTACGAATCAATCGAGGGAGGATTTATAAAAACAGGCATCATGGTTTCGTCAGCTATTGCGAAAAATGTGGAAATTCACAAGCTTTTTCATGGAGTGAGCTTGGACAGATGAGTTGTCCTTGCAATGATTCAAGGGTTTCTGAGTCACTTGTGGTTTCGGGACCTCTTTGGACTGGACCTCTACACAGTGCAGACTACATTGAAGACATGTTAACCTTGGCTAAGCAGTGGGAATGGATAGGCAATGGTCAGGGGAAGGATCTTGAGAAACTTCTGGGGCAGATGGTGGATGAGAGTGACTCCAAACTGCCAGTAGGGTACATCAAAATGGACGAGGTGGCAAGTCGAGCAAAAGTAAATTCACCTCCCCTCAATACCATGATGAGCGAGATGATCAAGGCTGGATATGCTGCTAGTAGGTCTCATATTGCCTCAAATGCAATCAAGACAAACTGTCCTATGGCAGAGTGCATCAGAATTGCTCTTGAACTGCGGCAGAATTGTCTTGGTGTGCATCAAGTCACATGA

Protein sequence

MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNCTERGIEFDIGDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDANDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPMAECIRIALELRQNCLGVHQVT

Homology

[Close]

BLAST of HG10014729 vs. NCBI nr
Match: XP_038892632.1 (tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida])

HSP 1 Score: 829.7 bits (2142), Expect = 1.2e-236
Identity = 408/441 (92.52%), Postives = 425/441 (96.37%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNC-----TERGIEFDI 60
           ML+V PRT S SPFLHSPQTNSHS  +STIHLFQFSHSN  SLNNC     TERG+EFDI
Sbjct: 1   MLSVLPRTPSPSPFLHSPQTNSHSLSASTIHLFQFSHSNKNSLNNCLPNNRTERGLEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           G TFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GGTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           ND+NRRVIVKNLS VAEDSGDARRW+VTNCDANRVMT FYL+KDFFDFID+DSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAEDSGDARRWMVTNCDANRVMTGFYLQKDFFDFIDIDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGR+YKNRH+GF+SYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGHYATPLFSYYSYHGPVFRVMLRINRGRVYKNRHYGFISYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDSRVS+SLVVSGPLWTGPLHSADYIE+ML+LAKQW+WIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDSRVSDSLVVSGPLWTGPLHSADYIENMLSLAKQWKWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MMDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELRQNCLGVHQVT 437
           AECIRIALEL+QNCLG HQVT
Sbjct: 421 AECIRIALELQQNCLGFHQVT 441

[Close]

BLAST of HG10014729 vs. NCBI nr
Match: XP_011655038.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >KGN50729.1 hypothetical protein Csa_005829 [Cucumis sativus])

HSP 1 Score: 812.4 bits (2097), Expect = 1.9e-231
Identity = 405/441 (91.84%), Postives = 420/441 (95.24%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNC-----TERGIEFDI 60
           MLTVAPRTLS SPFLH PQTNSH   SS+IHLFQFSHSN   LNNC     TERG++FDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           ND+NRRVIVKNLS VAE+ GD RRW+VTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI+KNRH+GFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIED+LTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELRQNCLGVHQVT 437
           AECIRIA +L++NCLG HQVT
Sbjct: 421 AECIRIA-QLQRNCLGAHQVT 440

[Close]

BLAST of HG10014729 vs. NCBI nr
Match: XP_008456934.1 (PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >XP_008456936.1 PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo] >TYK22417.1 tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 804.7 bits (2077), Expect = 4.0e-229
Identity = 401/441 (90.93%), Postives = 418/441 (94.78%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNC-----TERGIEFDI 60
           MLTVA  TLS SPFL+  QTNSH   S +IHLFQFSHSN KS NNC     TERG++FDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           ND+NRRVIVKNLS VAE+ GD RRW+VTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRI KNRH+GFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIED+LTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELRQNCLGVHQVT 437
           A+CIRIA +L+QNCLGVHQVT
Sbjct: 421 ADCIRIA-QLQQNCLGVHQVT 440

[Close]

BLAST of HG10014729 vs. NCBI nr
Match: XP_023529370.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 800.0 bits (2065), Expect = 9.9e-228
Identity = 399/441 (90.48%), Postives = 414/441 (93.88%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNC-----TERGIEFDI 60
           MLTV   TLS SPFL S QT SH F SSTIHLFQFS S +KSLNNC     TERGIEFDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQIKSLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           ND+NR VIVKNLS VAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSKVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRI+RGRI KNRH+GFVSYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIEDMLTLA QWEWIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELRQNCLGVHQVT 437
            ECIRIALEL+Q+CLGVHQVT
Sbjct: 421 TECIRIALELQQSCLGVHQVT 441

[Close]

BLAST of HG10014729 vs. NCBI nr
Match: XP_022943113.1 (tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata])

HSP 1 Score: 797.0 bits (2057), Expect = 8.3e-227
Identity = 398/441 (90.25%), Postives = 412/441 (93.42%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNC-----TERGIEFDI 60
           MLTV   TLS SPFL + QT SH F SSTIHLFQFS S  K LNNC     TERGIEFDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           ND+NR VIVKNLS VAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRINRGRI KNRH+GFVSYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIEDMLTLA QWEWIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELRQNCLGVHQVT 437
           AECIRIALEL+Q+CLGVHQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]

BLAST of HG10014729 vs. ExPASy Swiss-Prot
Match: B6YUU9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain NA1) OX=523850 GN=trm1 PE=3 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.5e-32
Identity = 120/379 (31.66%), Postives = 183/379 (48.28%), Query Frame = 0


Query: 59  FFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDANDD 118
           F+    A  RD+ VLA     K+ G  RVLDAL   GIR +RY +E  A  +  ND N +
Sbjct: 27  FYNPLMALNRDISVLAL----KALGSRRVLDALSATGIRGIRYALETPAGEIWLNDINPN 86

Query: 119 NRRVIVKNL----STVAEDSG------DARRWVVTNCDANRVMTEFYLKKDFFDFIDVDS 178
             R+IV N+    S   E  G        +  VVTN DANR+M E +    +FDF+D+D 
Sbjct: 87  AFRLIVDNVKLNFSGELEIEGKMAVLEGEKTLVVTNADANRLMNEQF---RYFDFVDLDP 146

Query: 179 FGSDSSFLRPAINALKLDGLLYVTSTD-GFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLR 238
           FGS   FL  A+ A+K  G+L +T+TD G   G +R +  L  Y A        +E GLR
Sbjct: 147 FGSPMEFLDSALRAVKRRGVLAITATDTGVLCGAYR-NACLRKYLAEPIRGELCHEAGLR 206

Query: 239 MLIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNS 298
           +LIG + R A+        L +YY  H   FR  L++  G    ++    + Y  +    
Sbjct: 207 ILIGTLVRYAAKYDLGVEVLLAYYRDH--YFRAFLQLRNGAKTADKSMKQLGYLYQ---- 266

Query: 299 QAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGK 358
                 E G+      ++ +       GPLW GPL + +++E++  L+ +   +G+   K
Sbjct: 267 -----DESGKFE--FENTFLPSKATAHGPLWLGPLKNQEFVEELHRLSAEHP-LGS---K 326

Query: 359 DLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASN 418
             +K LG + DE +  +P  Y     VA R  +    L  ++  + + GY A+R+H +  
Sbjct: 327 KTQKFLGLLKDELE--VPFFY-DTHAVARRHGLEVRKLAGVIEILYEKGYKATRTHFSPT 377

Query: 419 AIKTNCPMAECIRIALELR 427
           A+KT+ P  E +    EL+
Sbjct: 387 ALKTDAPFEEVLEALRELQ 377

[Close]

BLAST of HG10014729 vs. ExPASy Swiss-Prot
Match: Q9V1P3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=trm1 PE=3 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 2.1e-31
Identity = 106/369 (28.73%), Postives = 176/369 (47.70%), Query Frame = 0


Query: 59  FFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDANDD 118
           F+    A  RD+ V+   + +       VLDAL   GIR +R+ +E  A+ +  ND ++D
Sbjct: 27  FYNPRMALNRDIAVVLLNVLKPR----IVLDALSATGIRGIRFALETPAEEIWMNDISED 86

Query: 119 NRRVIVKNL-----STVAEDSGDA-----RRWVVTNCDANRVMTEFYLKKDFFDFIDVDS 178
              ++ KN+       + E +G A     +  VV + DANR+M E   K  +F FID+D 
Sbjct: 87  AYNLMKKNVLLNFKGELEESNGRAVLKSEKTLVVNHDDANRLMAE---KHRYFHFIDLDP 146

Query: 179 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 238
           FGS   FL  A+ ++K  G+L +T+TDG    G  P   +  Y A        +E+G R+
Sbjct: 147 FGSPMEFLDTALRSVKRKGILGITATDGAPLCGAHPKACMRKYLAVPLRGELCHEVGTRI 206

Query: 239 LIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKN---RHHGFVSYCEKCG 298
           L+G VAR A+        + +YY  H   FR  +++  G    +    + G++ + E  G
Sbjct: 207 LVGVVARYAAKYDLGIEVILAYYKDH--YFRAFIKLKDGARKGDESLENLGYIYFDESTG 266

Query: 299 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQ 358
                   E+ +   P   +         GP+W GPL S + +E+ML +++Q   +   +
Sbjct: 267 K------FEVERSFLPSKPN-------AYGPVWLGPLKSQEIVEEMLEISQQ---LSLAR 326

Query: 359 GKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 415
            K   KLL  + DE D  L   +     +  R K+ +  +  +++E+   GY ASR+H +
Sbjct: 327 KKQAVKLLKILKDELDVPL---FYDTHGLGRRLKIEARKIEEIINELRSLGYRASRTHFS 367

[Close]

BLAST of HG10014729 vs. ExPASy Swiss-Prot
Match: P81554 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=trm1 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 4.7e-31
Identity = 111/369 (30.08%), Postives = 171/369 (46.34%), Query Frame = 0


Query: 59  FFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDANDD 118
           F+    A  RD+ VL   + +       VLDAL   GIR +R+ +E  A+ +  ND N+ 
Sbjct: 29  FYNPRMAPNRDVVVLLLNVLKPK----IVLDALSATGIRGIRFALETPAEEIWMNDINEL 88

Query: 119 NRRVIVKNL-----STVAEDSGDA-----RRWVVTNCDANRVMTEFYLKKDFFDFIDVDS 178
              ++ KN+      T+ E++  A     +  V+ N DANR+M E   K  +F FID+D 
Sbjct: 89  AYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAE---KHRYFHFIDLDP 148

Query: 179 FGSDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRM 238
           FGS   FL  A+ ++K  G+L VT+TDG    G  P   L  Y A        +E+G R+
Sbjct: 149 FGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRI 208

Query: 239 LIGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKN---RHHGFVSYCEKCG 298
           L+G +AR A+        L +YY  H   FR  +++  G    +    + G+V + EK G
Sbjct: 209 LVGVIARYAAKYDLGMEVLLAYYKDH--YFRAFVKLKDGAKKGDETLENLGYVYFDEKTG 268

Query: 299 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQ 358
             +                S +S      GPLW GPL +   + +ML L      + N  
Sbjct: 269 RFEV-------------EKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVAN-- 328

Query: 359 GKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 415
            +++ KLL  + +E D  L   +     +  R K+    L  ++ E+   GY A+R+H +
Sbjct: 329 YREVLKLLHMLHEELDIPL---FYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFS 370

[Close]

BLAST of HG10014729 vs. ExPASy Swiss-Prot
Match: Q97ZH0 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=trm1 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.4e-29
Identity = 104/374 (27.81%), Postives = 190/374 (50.80%), Query Frame = 0


Query: 59  FFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEA-EADFVLANDAND 118
           F+  +    RDL V+  ++ +       ++DAL   GIR +RY VE+ +++ ++ ND N 
Sbjct: 35  FYNPKMTFNRDLSVIVVSILKPK----IIVDALSATGIRGIRYYVESWKSEQLILNDKNP 94

Query: 119 DNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLR 178
           +   +I  N    A+++G      + N DAN ++ E        D+ID+D FGS + F+ 
Sbjct: 95  NATSLIQIN----AKNNG-IENAKIYNKDANALLYEVK-----SDYIDIDPFGSPAPFIL 154

Query: 179 PAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREA 238
            ++NA   +G++  T+TD     G  P+     Y A    +  S E+GLR+LIG + REA
Sbjct: 155 SSLNAATRNGIVAFTATDLSPLEGSSPTSCRRKYDAINHKLSSSKELGLRVLIGKIIREA 214

Query: 239 SVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYK----NRHHGFVSYCEKCGNSQAFSWS 298
           ++L     PLFS+Y+ +   +R+ +R+  G        N+H  +   C +CG  Q F   
Sbjct: 215 AILEKTVYPLFSFYADY--YYRLFVRVENGARKADDNINKHLKYFGECPRCG-FQTFV-E 274

Query: 299 ELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLL 358
           E  +  CP       E   + GPL+ GPL+S ++++ ++ L   + ++ +     ++KLL
Sbjct: 275 ENCKTKCPV----CGEIFSIIGPLYIGPLYSMEFLKRIMDLYSNFNYLTS--FNRIQKLL 334

Query: 359 GQMVDESDSKLPVGYIKMDEVASRAKVNS-PPLNTMMSEMIKAGYAASRSHIASNAIKTN 418
              V E +++    +  + ++AS+ K+++ PP+ +++  +      ASR+H A   I+T+
Sbjct: 335 N--VIEKEARFKNVFYNISKLASKLKISAIPPIESILECL----GDASRTHFAPTGIRTD 378

Query: 419 CPMAECIRIALELR 427
               E  +    LR
Sbjct: 395 KEYEEITKCIKSLR 378

[Close]

BLAST of HG10014729 vs. ExPASy Swiss-Prot
Match: Q9YDY7 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=trm1 PE=3 SV=3)

HSP 1 Score: 130.2 bits (326), Expect = 5.8e-29
Identity = 105/380 (27.63%), Postives = 180/380 (47.37%), Query Frame = 0


Query: 57  DTFFRHESATGRDLGVLAAALYRKSKGHLR---VLDALCGCGIRSLRYLVEAEADFVLAN 116
           + F+       RD+ V+AA+  R++    R     DA  G G+R +RY VEA    V+ N
Sbjct: 39  EVFYNPAMEFNRDVSVVAASALRRTGLLTRSGVAFDAHAGVGVRGVRYAVEAGYVKVIMN 98

Query: 117 DANDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDF---FDFIDVDSFG 176
           D N     +   N      + G    +++ N ++N +M  F+L ++       ID+D +G
Sbjct: 99  DINPKASMLAALNARANGLEPGS---YMIFNKESNSLM--FHLSRERPTPVSLIDIDPYG 158

Query: 177 SDSSFLRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYG-AYVRPMPFSNEIGLRML 236
           S + F+  A+       ++ +T+TD     G +   ++  Y    V   P S E GLR+L
Sbjct: 159 SPAPFVDAALALSGKGTVVAMTATDLAVLEGGKARAAVRRYMLRSVSKTPVSKETGLRVL 218

Query: 237 IGGVAREASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKN----RHHGFVSYCEKCG 296
           +G VAR A+       PL +YY+ H   +RV + + RG    +     + G + YC + G
Sbjct: 219 LGYVARVAAAHDKAVKPLLAYYADH--YYRVYVAVERGARRSDSMLEENLGRLVYCPETG 278

Query: 297 NSQAFSWSELGQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQ 356
            + A S++E    +  C  S V    V + P W G L    ++E ML +A +  W+G   
Sbjct: 279 VALALSYAE--DPASACGGSYV----VAADPAWIGSLGDQAFLEAMLNIAVEAVWLGT-- 338

Query: 357 GKDLEKLLGQMVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIA 416
              +EKLL  +  E+       Y+ +  VAS+A+VN+P  + ++  +   GY A  +H +
Sbjct: 339 RPRVEKLLNTLHGEAPLSPRSIYVSLTSVASKARVNTPKKSKVVELLRSMGYRAVATHFS 398

Query: 417 SNAIKTNCPMAECIRIALEL 426
              ++T+ P  E +   ++L
Sbjct: 399 GEGVRTDAPWGEVLEAVVKL 403

[Close]

BLAST of HG10014729 vs. ExPASy TrEMBL
Match: A0A0A0KP99 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G220920 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 9.3e-232
Identity = 405/441 (91.84%), Postives = 420/441 (95.24%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNC-----TERGIEFDI 60
           MLTVAPRTLS SPFLH PQTNSH   SS+IHLFQFSHSN   LNNC     TERG++FDI
Sbjct: 1   MLTVAPRTLSPSPFLHIPQTNSHFLTSSSIHLFQFSHSNKIPLNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGRLRVLDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           ND+NRRVIVKNLS VAE+ GD RRW+VTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSRVAENIGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+YATPLFSYYSYHGPVFRVMLRINRGRI+KNRH+GFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGFYATPLFSYYSYHGPVFRVMLRINRGRIHKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIED+LTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLRQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELRQNCLGVHQVT 437
           AECIRIA +L++NCLG HQVT
Sbjct: 421 AECIRIA-QLQRNCLGAHQVT 440

[Close]

BLAST of HG10014729 vs. ExPASy TrEMBL
Match: A0A1S3C509 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496736 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 1.9e-229
Identity = 401/441 (90.93%), Postives = 418/441 (94.78%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNC-----TERGIEFDI 60
           MLTVA  TLS SPFL+  QTNSH   S +IHLFQFSHSN KS NNC     TERG++FDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           ND+NRRVIVKNLS VAE+ GD RRW+VTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRI KNRH+GFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIED+LTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELRQNCLGVHQVT 437
           A+CIRIA +L+QNCLGVHQVT
Sbjct: 421 ADCIRIA-QLQQNCLGVHQVT 440

[Close]

BLAST of HG10014729 vs. ExPASy TrEMBL
Match: A0A5D3DFE8 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold530G00120 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 1.9e-229
Identity = 401/441 (90.93%), Postives = 418/441 (94.78%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNC-----TERGIEFDI 60
           MLTVA  TLS SPFL+  QTNSH   S +IHLFQFSHSN KS NNC     TERG++FDI
Sbjct: 1   MLTVASTTLSPSPFLYIQQTNSHFLTSPSIHLFQFSHSNKKSPNNCLSNNRTERGLDFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKGHLR+LDALCGCGIRSLRYLVE+EADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRILDALCGCGIRSLRYLVESEADFVLANDA 120

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           ND+NRRVIVKNLS VAE+ GD RRW+VTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF
Sbjct: 121 NDENRRVIVKNLSKVAENFGDNRRWLVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAY+RPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYIRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRI KNRH+GFVSYCEKCGNSQAFSWSEL
Sbjct: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRISKNRHYGFVSYCEKCGNSQAFSWSEL 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCNDSRVSESLVVSGPLW GPLHSADYIED+LTLAKQWEWIGNGQGKDLEKLL Q
Sbjct: 301 GQMSCPCNDSRVSESLVVSGPLWIGPLHSADYIEDILTLAKQWEWIGNGQGKDLEKLLMQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M+DESDSKLPVGYIKMDEVASRAKVNSPPL+TMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MLDESDSKLPVGYIKMDEVASRAKVNSPPLSTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELRQNCLGVHQVT 437
           A+CIRIA +L+QNCLGVHQVT
Sbjct: 421 ADCIRIA-QLQQNCLGVHQVT 440

[Close]

BLAST of HG10014729 vs. ExPASy TrEMBL
Match: A0A6J1FWD4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111447941 PE=3 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 4.0e-227
Identity = 398/441 (90.25%), Postives = 412/441 (93.42%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNC-----TERGIEFDI 60
           MLTV   TLS SPFL + QT SH F SSTIHLFQFS S  K LNNC     TERGIEFDI
Sbjct: 1   MLTVMLNTLSPSPFLRTQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           ND+NR VIVKNLS VAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRINRGRI KNRH+GFVSYC+KCGNSQAFSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRINRGRINKNRHYGFVSYCKKCGNSQAFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIEDMLTLA QWEWIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELRQNCLGVHQVT 437
           AECIRIALEL+Q+CLGVHQ T
Sbjct: 421 AECIRIALELQQSCLGVHQAT 441

[Close]

BLAST of HG10014729 vs. ExPASy TrEMBL
Match: A0A6J1JBD3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484232 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 6.9e-227
Identity = 398/441 (90.25%), Postives = 412/441 (93.42%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFLHSPQTNSHSFFSSTIHLFQFSHSNVKSLNNC-----TERGIEFDI 60
           MLTV   TLS SPFL S QT SH F SSTIHLFQFS S  K LNNC     TERGIEFDI
Sbjct: 1   MLTVMLNTLSPSPFLRSQQTISHCFSSSTIHLFQFSQSQKKPLNNCLPNNRTERGIEFDI 60

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           GDTFFRHESATGRDLGVLAAALYRKSKG LRVLDALCGCGIRSLRYLVEAEADFVLANDA
Sbjct: 61  GDTFFRHESATGRDLGVLAAALYRKSKGSLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           ND+NR VIVKNLS VAE  G+ RRW+VTNCDANRVM+EFYL+KDFFDFIDVDSFGSDS+F
Sbjct: 121 NDENRGVIVKNLSRVAEGFGETRRWMVTNCDANRVMSEFYLQKDFFDFIDVDSFGSDSTF 180

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LRPAINALKLDGLLYVTSTDGFSSGGH PSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR
Sbjct: 181 LRPAINALKLDGLLYVTSTDGFSSGGHHPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EASVLG+Y TPLFSYYSYHGPVFRVMLRI+RGRI KNRH+GFVSYC+KCGNSQ FSWSE+
Sbjct: 241 EASVLGFYVTPLFSYYSYHGPVFRVMLRISRGRINKNRHYGFVSYCKKCGNSQGFSWSEV 300

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           GQMSCPCND+RVSESLVVSGPLWTGPLHSADYIEDMLTLA QWEWIGNGQGKDLEKLLGQ
Sbjct: 301 GQMSCPCNDTRVSESLVVSGPLWTGPLHSADYIEDMLTLAMQWEWIGNGQGKDLEKLLGQ 360

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM
Sbjct: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420

Query: 421 AECIRIALELRQNCLGVHQVT 437
           AECIRIALEL+Q+CLGVHQVT
Sbjct: 421 AECIRIALELQQSCLGVHQVT 441

[Close]

BLAST of HG10014729 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 557.4 bits (1435), Expect = 1.0e-158
Identity = 273/429 (63.64%), Postives = 333/429 (77.62%), Query Frame = 0


Query: 1   MLTVAPRTLSSSPFL-----HSPQTNSHSFFSSTIHLFQFSHSNVKSLNNCTERGIEFDI 60
           +LT++P+TLSSS F       +P+  S            F    VKS     ER +EF+ 
Sbjct: 2   LLTLSPKTLSSSSFFTVHKSQNPKCKSPDSCRFKSLKCSFDRILVKS-EVQIERNLEFET 61

Query: 61  GDTFFRHESATGRDLGVLAAALYRKSKGHLRVLDALCGCGIRSLRYLVEAEADFVLANDA 120
           G+TFFRHESA GRDLGVL+A LY++S G LRVLDA+CGCGIRSLRYLVEAEADFV+ANDA
Sbjct: 62  GETFFRHESARGRDLGVLSATLYKRSNGSLRVLDAMCGCGIRSLRYLVEAEADFVMANDA 121

Query: 121 NDDNRRVIVKNLSTVAEDSGDARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSF 180
           NDDNRRVI  NLS V   +GD RRWVVT+  AN+ M E Y+  DFFD ID+DSFGSDSSF
Sbjct: 122 NDDNRRVITDNLSKVERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSF 181

Query: 181 LRPAINALKLDGLLYVTSTDGFSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAR 240
           LR A NAL+L GLLY+TSTDG+SSGGHRP  SLAAYGA++RPMPF NEIGLRMLIGG  R
Sbjct: 182 LRDAFNALRLGGLLYLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVR 241

Query: 241 EASVLGYYATPLFSYYSYHGPVFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSEL 300
           EA++LGY+ TPLFSYYSYHGPVFRVMLR++RG+++++R++GFV++C  CG+S    + EL
Sbjct: 242 EAALLGYHVTPLFSYYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFDEL 301

Query: 301 GQMSCPCNDSRVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQ 360
           G M CPC+D++ S SLVVSGP+W GPLH A Y+ +ML LAK+W W+  G G DL+KLL  
Sbjct: 302 GLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLSI 361

Query: 361 MVDESDSKLPVGYIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 420
           M++ESD +LP GY KMDE+ASRAK+NSPPL  MMS ++K GYAASRSHI  NA+KT+CPM
Sbjct: 362 MIEESDPRLPPGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDCPM 421

Query: 421 AECIRIALE 425
           +  +RIA E
Sbjct: 422 SHFVRIAKE 429

[Close]

BLAST of HG10014729 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 84.7 bits (208), Expect = 2.0e-16
Identity = 85/356 (23.88%), Postives = 147/356 (41.29%), Query Frame = 0


Query: 86  RVLDALCGCGIRSLRYLVEAEA-DFVLANDANDDNRRVIVKN--------LSTVAEDSGD 145
           +VL+AL   G+R+LRY  E E    V+A D +  +     +N        +S V     D
Sbjct: 123 KVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVAISKVESHHTD 182

Query: 146 ARRWVVTNCDANRVMTEFYLKKDFFDFIDVDSFGSDSSFLRPAINALKLDGLLYVTSTDG 205
           AR  ++T+                FD +D+D +GS S FL  AI ++   GLL  T+TD 
Sbjct: 183 ARVHMLTH-------------PKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDM 242

Query: 206 FSSGGHRPSQSLAAYGAYVRPMPFSNEIGLRMLIGGVAREASVLGYYATPLFSYYSYHGP 265
               G       + YG+Y     + +E+ LR+L+  +   A+    Y  P+ S       
Sbjct: 243 AVLCGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDF-- 302

Query: 266 VFRVMLRINRGRIYKNRHHGFVSYCEKCGNSQAFSWSELGQMSCPCNDS----------- 325
             RV +R+             +SY  +C    +F    +G+ S P N+S           
Sbjct: 303 YVRVFVRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGR-SLPKNNSVRYLPAIGPVV 362

Query: 326 -----RVSESLVVSGPLWTGPLHSADYIEDMLTLAKQWEWIGNGQGKDLEKLLGQMVDES 385
                   +   + GP+W+ P+H  +++  +L   K  +     +    +++   +   S
Sbjct: 363 KQDCNHCGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMK----DRYPAYDRISAVLTTVS 422

Query: 386 DSKLPVG-YIKMDEVASRAKVNSPPLNTMMSEMIKAGYAASRSHIASNAIKTNCPM 416
           +  L V  ++ +  + +  K  SP      S +I A Y  S +H+    +KT+ PM
Sbjct: 423 EELLDVPLFLSLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPM 458

The following BLAST results are available for this feature:

BLAST of HG10014729 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892632.1	1.2e-236	92.52	tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Benincasa hispida]	[more]
XP_011655038.1	1.9e-231	91.84	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucumis sativus] >XP_011655039.1 tR...	[more]
XP_008456934.1	4.0e-229	90.93	PREDICTED: tRNA (guanine(26)-N(2))-dimethyltransferase isoform X1 [Cucumis melo]...	[more]
XP_023529370.1	9.9e-228	90.48	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita pepo subsp. pepo]	[more]
XP_022943113.1	8.3e-227	90.25	tRNA (guanine(26)-N(2))-dimethyltransferase [Cucurbita moschata]	[more]

BLAST of HG10014729 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B6YUU9	1.5e-32	31.66	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Thermococcus onnurineus (strain N...	[more]
Q9V1P3	2.1e-31	28.73	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus abyssi (strain GE5 / O...	[more]
P81554	4.7e-31	30.08	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Pyrococcus furiosus (strain ATCC ...	[more]
Q97ZH0	3.4e-29	27.81	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Saccharolobus solfataricus (strai...	[more]
Q9YDY7	5.8e-29	27.63	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Aeropyrum pernix (strain ATCC 700...	[more]

BLAST of HG10014729 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KP99	9.3e-232	91.84	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A1S3C509	1.9e-229	90.93	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103496...	[more]
A0A5D3DFE8	1.9e-229	90.93	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A6J1FWD4	4.0e-227	90.25	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1JBD3	6.9e-227	90.25	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]

BLAST of HG10014729 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56330.1	1.0e-158	63.64	N2,N2-dimethylguanosine tRNA methyltransferase	[more]
AT3G02320.1	2.0e-16	23.88	N2,N2-dimethylguanosine tRNA methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002905	tRNA methyltransferase, Trm1	PFAM	PF02005	TRM	coord: 59..421 e-value: 4.7E-65 score: 220.2
IPR002905	tRNA methyltransferase, Trm1	PANTHER	PTHR10631	N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE	coord: 10..427
IPR002905	tRNA methyltransferase, Trm1	PROSITE	PS51626	SAM_MT_TRM1	coord: 44..422 score: 96.185844
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 34..362 e-value: 1.9E-74 score: 252.3
None	No IPR available	PANTHER	PTHR10631:SF9	TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE	coord: 10..427
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 86..192 e-value: 2.5306E-4 score: 38.1799
IPR042296	tRNA methyltransferase, Trm1, C-terminal	GENE3D	3.30.56.70		coord: 367..423 e-value: 3.3E-16 score: 60.7
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 41..422

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10014729.1	HG10014729.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002940	tRNA N2-guanine methylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0004809	tRNA (guanine-N2-)-methyltransferase activity
molecular_function	GO:0003723	RNA binding