Moc10g02820 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g02820
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionpre-mRNA-splicing factor CWC25 homolog
Locationchr10: 1943938 .. 1945998 (-)
RNA-Seq ExpressionMoc10g02820
SyntenyMoc10g02820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGTTGAAGTTTCTGAACAAGAAAGGATGGCACACCGGTAGTCTCCGGAACATAGAAAACGTGTGGAAGGCGGAGCAAAAGCACGAGGCTGAGCAGAAGAAGTTGGACGAGCTTCGAAAGCAGATCCAGGAGGAGAAAGAGCGCCAAGAGTTTCGTCTTCTTCAAGAACAGGCCGGACTTGTTCCGTATGTTTTTCTTTTTTCTTTCTTCTCTGTCCTCTATCCTTTAGTTTGCAGATTGTATATTTAATTGCTAGTCTATGAGTTTCAAATTTGATTGCTGAATGGATGAAGATTGAATTGAAGGTGTGCCCTAGGTTAGAGTTATTGTGCAGCGATGGATTAGGGTTTAATTTGTGCAACTGGTTTTACTAATATTCGATTTTTTCGTCGATTATGGTGTTCTAGGAAGCAAGAGAGATTGGATTTTCTTTATGAGTCGGGTTTGGCAGTCGGGAAAGGTAGTAGTTCTGATGGATTTAAGAGTCTCGAAACCCTGCCAAGTACATCTGCTATTGTGGCCACCACTGAACCATCTAGCTCCAAGGTATTTTCTCTCTTGTTGGCTTTGTTTTGTTAACTTGTGTGCTCTTGCTATCTCTTCACACTGCAGGTTTGACCTTGACACACTTTTGTGTTGTTTCTGCAGGAAGCTGCTGCTCCCGGAGCTTTGTTTGAGAACAAGCCTCATTCTGCCAATGATTCATGGAGGAAGCTCCATTCTGATCCTTTGCTTATCATTCGGCAGCGTGAGCAAGAAGCTCTTGCACGTGTAAAGAATAACCCCATTCAGATGGCTATGATTCGCAAAACTGTAAGTTCACATGTTTTTTCTCCTACTGTTTTATCTTCTCCATATGTTGCTTTAAACGCTATTAAGTTCTAGGACTTTTATTGTTATAACAGGGGCCATGGAAATTATATGGAAATATCCTTTCATTCAGTATTTGTCTGGGAAATTCTCTGAACTTTTCTATAGACACTACTATGTGGTAGAGAAAGTTGGTGGCTTATCTTAATTCTAAGTTATCCTTTTGAGTTGATGTAATGGATATCTCTGCATGTCTGACCCTTAGGATAGGAGCTAAAGATGAATCTATGATCTGAAATTTCTTTCTATATATCGTCATGACCGGTCAATAGTCTAGAAAGTGGAAGCTTTTGTTAAAAGAATTTAGAATGTTCCATATGATCATTACGGTTCTTGTGATAGAATATCTCTTTCAACTGATATCGTTGAATGCTGCAAGCTCTTTCCCTTATTTGACTTTAAGTTTGGACCTGAGGATTCCATTTAATATATATGTAGGTTAAAGTGGAAAAACACAAGGAAAAGAATCCTGATGACAAAAGAAAACGAAAGAAGCACAACCATAGTAAATCCAAACGTCATAAAGATTTTTCTTCCGAAAGAGATTATGATTCTGAAAACGCCAGCCCTGAGAGGCAGAGGAGACATGATCGTGACAAAAGTTCAAAACACGATCATTTTTATTCAGAAGATAGAAGGAGCAAAACTGATTTTAAGAATGAGAGGGTTCATGACAGAGGCTCGAAATATGCTGCACATTCGACTGATCAGTCAGATCGAAAGACGTCCAAAACCAGTACTCATGGTTCTGCAGAGAGACATCATGACAGATCAAAGAGAGAGAGAGATTCTTATGCTAGCAGTGAAAAGAACAGCAGAAGAGTAGGGGAATTGAGATCTTATGAATCTAATTCTTCTGAAACTCCTCACGAGTCACGCCATAAGCATCGTAGGACCATTAATAAGCTTTCAGAAGAAGAGCGTGCAGCTAGGCTGCGGGAGATGCAGCAGGATGCTGAGTTACACGATGAGCAAAGATGGAGGCGTTTGAAGAAGGCAGCTGAGGATGATGCCCTAGAAGCCAAACAAGCTGCAGAGCCATCAGGCAAAAATTTCTTGGATGCTGCTCAGAAACGAATGTATGGTGCTGAGAAGGGAGGAAGCTCAACAATCGAGGAGAGTATTCGCCGTAGGACGTACTATTCACAGGGTAAATCTGAAATTGAAGCCAATGCTTTTCGAAGGTGA

mRNA sequence

ATGGCGTTGAAGTTTCTGAACAAGAAAGGATGGCACACCGGTAGTCTCCGGAACATAGAAAACGTGTGGAAGGCGGAGCAAAAGCACGAGGCTGAGCAGAAGAAGTTGGACGAGCTTCGAAAGCAGATCCAGGAGGAGAAAGAGCGCCAAGAGTTTCGTCTTCTTCAAGAACAGGCCGGACTTGTTCCGAAGCAAGAGAGATTGGATTTTCTTTATGAGTCGGGTTTGGCAGTCGGGAAAGGTAGTAGTTCTGATGGATTTAAGAGTCTCGAAACCCTGCCAAGTACATCTGCTATTGTGGCCACCACTGAACCATCTAGCTCCAAGGAAGCTGCTGCTCCCGGAGCTTTGTTTGAGAACAAGCCTCATTCTGCCAATGATTCATGGAGGAAGCTCCATTCTGATCCTTTGCTTATCATTCGGCAGCGTGAGCAAGAAGCTCTTGCACGTGTAAAGAATAACCCCATTCAGATGGCTATGATTCGCAAAACTGTTAAAGTGGAAAAACACAAGGAAAAGAATCCTGATGACAAAAGAAAACGAAAGAAGCACAACCATAGTAAATCCAAACGTCATAAAGATTTTTCTTCCGAAAGAGATTATGATTCTGAAAACGCCAGCCCTGAGAGGCAGAGGAGACATGATCGTGACAAAAGTTCAAAACACGATCATTTTTATTCAGAAGATAGAAGGAGCAAAACTGATTTTAAGAATGAGAGGGTTCATGACAGAGGCTCGAAATATGCTGCACATTCGACTGATCAGTCAGATCGAAAGACGTCCAAAACCAGTACTCATGGTTCTGCAGAGAGACATCATGACAGATCAAAGAGAGAGAGAGATTCTTATGCTAGCAGTGAAAAGAACAGCAGAAGAGTAGGGGAATTGAGATCTTATGAATCTAATTCTTCTGAAACTCCTCACGAGTCACGCCATAAGCATCGTAGGACCATTAATAAGCTTTCAGAAGAAGAGCGTGCAGCTAGGCTGCGGGAGATGCAGCAGGATGCTGAGTTACACGATGAGCAAAGATGGAGGCGTTTGAAGAAGGCAGCTGAGGATGATGCCCTAGAAGCCAAACAAGCTGCAGAGCCATCAGGCAAAAATTTCTTGGATGCTGCTCAGAAACGAATGTATGGTGCTGAGAAGGGAGGAAGCTCAACAATCGAGGAGAGTATTCGCCGTAGGACGTACTATTCACAGGGTAAATCTGAAATTGAAGCCAATGCTTTTCGAAGGTGA

Coding sequence (CDS)

ATGGCGTTGAAGTTTCTGAACAAGAAAGGATGGCACACCGGTAGTCTCCGGAACATAGAAAACGTGTGGAAGGCGGAGCAAAAGCACGAGGCTGAGCAGAAGAAGTTGGACGAGCTTCGAAAGCAGATCCAGGAGGAGAAAGAGCGCCAAGAGTTTCGTCTTCTTCAAGAACAGGCCGGACTTGTTCCGAAGCAAGAGAGATTGGATTTTCTTTATGAGTCGGGTTTGGCAGTCGGGAAAGGTAGTAGTTCTGATGGATTTAAGAGTCTCGAAACCCTGCCAAGTACATCTGCTATTGTGGCCACCACTGAACCATCTAGCTCCAAGGAAGCTGCTGCTCCCGGAGCTTTGTTTGAGAACAAGCCTCATTCTGCCAATGATTCATGGAGGAAGCTCCATTCTGATCCTTTGCTTATCATTCGGCAGCGTGAGCAAGAAGCTCTTGCACGTGTAAAGAATAACCCCATTCAGATGGCTATGATTCGCAAAACTGTTAAAGTGGAAAAACACAAGGAAAAGAATCCTGATGACAAAAGAAAACGAAAGAAGCACAACCATAGTAAATCCAAACGTCATAAAGATTTTTCTTCCGAAAGAGATTATGATTCTGAAAACGCCAGCCCTGAGAGGCAGAGGAGACATGATCGTGACAAAAGTTCAAAACACGATCATTTTTATTCAGAAGATAGAAGGAGCAAAACTGATTTTAAGAATGAGAGGGTTCATGACAGAGGCTCGAAATATGCTGCACATTCGACTGATCAGTCAGATCGAAAGACGTCCAAAACCAGTACTCATGGTTCTGCAGAGAGACATCATGACAGATCAAAGAGAGAGAGAGATTCTTATGCTAGCAGTGAAAAGAACAGCAGAAGAGTAGGGGAATTGAGATCTTATGAATCTAATTCTTCTGAAACTCCTCACGAGTCACGCCATAAGCATCGTAGGACCATTAATAAGCTTTCAGAAGAAGAGCGTGCAGCTAGGCTGCGGGAGATGCAGCAGGATGCTGAGTTACACGATGAGCAAAGATGGAGGCGTTTGAAGAAGGCAGCTGAGGATGATGCCCTAGAAGCCAAACAAGCTGCAGAGCCATCAGGCAAAAATTTCTTGGATGCTGCTCAGAAACGAATGTATGGTGCTGAGAAGGGAGGAAGCTCAACAATCGAGGAGAGTATTCGCCGTAGGACGTACTATTCACAGGGTAAATCTGAAATTGAAGCCAATGCTTTTCGAAGGTGA

Protein sequence

MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFENKPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRKRKKHNHSKSKRHKDFSSERDYDSENASPERQRRHDRDKSSKHDHFYSEDRRSKTDFKNERVHDRGSKYAAHSTDQSDRKTSKTSTHGSAERHHDRSKRERDSYASSEKNSRRVGELRSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDALEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR
Homology
BLAST of Moc10g02820 vs. NCBI nr
Match: XP_022141219.1 (pre-mRNA-splicing factor CWC25 homolog [Momordica charantia])

HSP 1 Score: 752.3 bits (1941), Expect = 2.2e-213
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120
           LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120

Query: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180
           KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK
Sbjct: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180

Query: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRRHDRDKSSKHDHFYSEDRRSKTDFKNER 240
           RKKHNHSKSKRHKDFSSERDYDSENASPERQRRHDRDKSSKHDHFYSEDRRSKTDFKNER
Sbjct: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRRHDRDKSSKHDHFYSEDRRSKTDFKNER 240

Query: 241 VHDRGSKYAAHSTDQSDRKTSKTSTHGSAERHHDRSKRERDSYASSEKNSRRVGELRSYE 300
           VHDRGSKYAAHSTDQSDRKTSKTSTHGSAERHHDRSKRERDSYASSEKNSRRVGELRSYE
Sbjct: 241 VHDRGSKYAAHSTDQSDRKTSKTSTHGSAERHHDRSKRERDSYASSEKNSRRVGELRSYE 300

Query: 301 SNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDALEAK 360
           SNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDALEAK
Sbjct: 301 SNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDALEAK 360

Query: 361 QAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 414
           QAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR
Sbjct: 361 QAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 413

BLAST of Moc10g02820 vs. NCBI nr
Match: XP_038896474.1 (pre-mRNA-splicing factor CWC25 homolog [Benincasa hispida])

HSP 1 Score: 627.5 bits (1617), Expect = 8.3e-176
Identity = 361/417 (86.57%), Postives = 381/417 (91.37%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120
           LVPKQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKEAA PGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHKEKNPD KR+
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKEKNPDGKRE 180

Query: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRR-HDRDKSSKHD-HFYSEDRRSKTDFKN 240
           RKKH HSKSKRHKD SSERDYDSE+ASPERQRR HD + SSKHD H + EDRRSK D KN
Sbjct: 181 RKKHRHSKSKRHKDSSSERDYDSEDASPERQRRKHDHENSSKHDAHSHLEDRRSKADSKN 240

Query: 241 ERVHDRGSKYAAHST-DQSDRKTSKTSTHGS-AERHHDRSKRERDSYASSEKNSRRVGEL 300
           ER  DRGSKYAA +T DQSDR+TSK++ H S AERHHDRS+R+RDSYA++EKNSRRVGE 
Sbjct: 241 ERDRDRGSKYAARTTYDQSDRRTSKSNPHDSAAERHHDRSRRDRDSYANNEKNSRRVGEP 300

Query: 301 RSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDA 360
           RSY SN+SET HESRHKHRR   KLSEEER ARL EMQQDAELH+EQRWRRLKKAAEDDA
Sbjct: 301 RSYGSNASET-HESRHKHRRPATKLSEEERVARLHEMQQDAELHEEQRWRRLKKAAEDDA 360

Query: 361 LEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 414
           LEAKQ A PS +NFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR
Sbjct: 361 LEAKQNAAPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 416

BLAST of Moc10g02820 vs. NCBI nr
Match: XP_008453851.1 (PREDICTED: pre-mRNA-splicing factor CWC25 homolog [Cucumis melo] >KAA0044681.1 pre-mRNA-splicing factor CWC25-like protein [Cucumis melo var. makuwa] >TYK16902.1 pre-mRNA-splicing factor CWC25-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 621.3 bits (1601), Expect = 5.9e-174
Identity = 358/419 (85.44%), Postives = 384/419 (91.65%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120
           LVPKQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKEAAAPGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHK+KNPDDKR+
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRR-HDRDKSSKHD-HFYSEDRRSKTDFKN 240
           RKKH HSKSKRHKD S ERDYDSE+ASPERQRR HD DKSS+HD H +SEDRRSK D KN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240

Query: 241 ERVHDRGSKYAAH-STDQSDRKTSKTSTHGS-AERHHDRSK--RERDSYASSEKNSRRVG 300
           ER  DRGSKYAA  S DQSDRKTSK++   S A+RHHDRSK  R+RDS+A+++K+SRRVG
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVG 300

Query: 301 ELRSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAED 360
           E R YES++SETP+ESRH+HRR   KLSEEERAARLREMQQDAELH+EQRW+RLKKAAED
Sbjct: 301 EPRYYESHASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360

Query: 361 DALEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 414
           DALEAKQ A PS +NFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR
Sbjct: 361 DALEAKQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 419

BLAST of Moc10g02820 vs. NCBI nr
Match: XP_004146897.1 (pre-mRNA-splicing factor CWC25 homolog [Cucumis sativus] >KGN53175.1 hypothetical protein Csa_014942 [Cucumis sativus])

HSP 1 Score: 615.5 bits (1586), Expect = 3.3e-172
Identity = 352/417 (84.41%), Postives = 381/417 (91.37%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120
           LVPKQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKEAA PGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHK+KNPDDKR+
Sbjct: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRR-HDRDKSSKHD-HFYSEDRRSKTDFKN 240
           RKKH HSKSKRHKD S ERDYDSE+ SPERQRR HD DKSS+HD H +SEDRRSK + KN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRRKHDHDKSSRHDGHSHSEDRRSKAETKN 240

Query: 241 ERVHDRGSKYAAH-STDQSDRKTSKTSTHGS-AERHHDRSKRERDSYASSEKNSRRVGEL 300
           ER  DRGSKYAA  S DQSDRKT K++ H S A+R+HDRSKR+RDSYA+++++SRRVGEL
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRYHDRSKRDRDSYATNDRDSRRVGEL 300

Query: 301 RSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDA 360
           R YESN+SETP+ESRH+HRR   KLSEEERAARLREMQQDAELH+EQR++RLKKA EDDA
Sbjct: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360

Query: 361 LEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 414
           LEAKQ A PS +NFLD AQKRMY AEKGGSSTIEESIRRRTYYSQGKS+IEANAFRR
Sbjct: 361 LEAKQNAVPSSRNFLDMAQKRMYSAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 417

BLAST of Moc10g02820 vs. NCBI nr
Match: XP_023551879.1 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 590.1 bits (1520), Expect = 1.5e-164
Identity = 343/417 (82.25%), Postives = 367/417 (88.01%), Query Frame = 0

Query: 2   ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGL 61
           ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAGL
Sbjct: 7   ALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAGL 66

Query: 62  VPKQERLDFLYESGLAVGKGSSSDGFKSLETLP-STSAIVATTEPSSSKEAAAPGALFEN 121
           VPKQERLDFLYESGLAVGKGS+SDGFKSLETLP +TS   A TEPSSSKEAAAPGALFE+
Sbjct: 67  VPKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAATEPSSSKEAAAPGALFED 126

Query: 122 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 181
           KPHSANDSWRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHK+KNPDDKR+
Sbjct: 127 KPHSANDSWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 186

Query: 182 RKKHNHSKSKRHKDFSSERDYDSENASPERQRR-HDRDKSSKHD-HFYSEDRRSKTDFKN 241
           RKKH HSKSKR K  S ERDYDSE+ SPE QRR HDRDKSSKHD H YSEDRRSKTD+ N
Sbjct: 187 RKKHRHSKSKRRKASSPERDYDSESVSPEMQRRNHDRDKSSKHDVHSYSEDRRSKTDYVN 246

Query: 242 ERVHDRGSKYAAHSTDQSDRKTSKTSTHG--SAERHHDRSKRERDSYASSEKNSRRVGEL 301
           ER  DRGSKY     DQSDRKT K++ H   + ERHHD  KR+RDSY  +EK+S +VGE 
Sbjct: 247 ERDRDRGSKY-----DQSDRKTPKSNPHDYYAEERHHDSXKRDRDSYTHNEKDSTKVGES 306

Query: 302 RSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDA 361
           R+ E N+S++P+E RHKHRR   KLSEE RAARLREMQQDAE H+EQRWRRLKKAAEDDA
Sbjct: 307 RTREPNASKSPYEPRHKHRRPATKLSEEGRAARLREMQQDAEFHEEQRWRRLKKAAEDDA 366

Query: 362 LEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 414
           LEA+Q A PS +NFLD AQKRMYGAEKGGSSTIEESI  RTYYSQGKSEIEANAFRR
Sbjct: 367 LEAEQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESICWRTYYSQGKSEIEANAFRR 418

BLAST of Moc10g02820 vs. ExPASy Swiss-Prot
Match: Q9NXE8 (Pre-mRNA-splicing factor CWC25 homolog OS=Homo sapiens OX=9606 GN=CWC25 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.8e-17
Identity = 136/434 (31.34%), Postives = 213/434 (49.08%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLV-PKQE 67
           KK WH  +LRN+E VWKAEQKHEAE+KK++EL+++++EE+ R+E +   E  G V  K+E
Sbjct: 9   KKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEE 68

Query: 68  RLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPS----SSKEAAAPGALFENKP 127
           +LD++Y+     G G   +  + L   P    +    E      SS+    PG++F   P
Sbjct: 69  KLDWMYQ-----GPGGMVNRDEYLLGRPIDKYVFEKMEEKEAGCSSETGLLPGSIF--AP 128

Query: 128 HSAN---DSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKR 187
             AN   D   K+  DPL IIR++E+E    V NNP++M  I++ +++   K++    K 
Sbjct: 129 SGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQMSLEKKEKKKKKE 188

Query: 188 KRKKHNHSKSKRHKDFSSERDYDSENASPERQRRHDRDKS---SKHDHFYSEDRRSKTD- 247
           K+KKH   K  +H+  SS+R    +  S  R ++   + S   SK   +  + R S  + 
Sbjct: 189 KKKKH---KKHKHRSSSSDRSSSEDEHSAGRSQKKMANSSPVLSKVPGYGLQVRNSDRNQ 248

Query: 248 -----FKNERVHDRGSKYAAHSTDQS---DRKTSKTSTH--GSAERHH----DRSKRERD 307
                   E+    G K  + S   S    R  SK ST   GS +R       RS+  R 
Sbjct: 249 GLQGPLTAEQKRGHGMKNHSRSRSSSHSPPRHASKKSTREAGSRDRRSRSLGRRSRSPRP 308

Query: 308 SYASSEK-NSRRVGELRSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELH 367
           S   + K N R  G+ RS   +  +  ++ RH    T  KLS EE   + +EM ++A+  
Sbjct: 309 SKLHNSKVNRRETGQTRS--PSPKKEVYQRRHAPGYT-RKLSAEELERKRQEMMENAKWR 368

Query: 368 DEQRWRRLKKAAEDDALEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYS 414
           +E+R   LK+ A+D+  E +Q  E       D         E   +S++E+ ++R  Y  
Sbjct: 369 EEERLNILKRHAKDE--EREQRLEKLDSR--DGKFIHRMKLESASTSSLEDRVKRNIYSL 425

BLAST of Moc10g02820 vs. ExPASy Swiss-Prot
Match: Q9DBF7 (Pre-mRNA-splicing factor CWC25 homolog OS=Mus musculus OX=10090 GN=Cwc25 PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 1.8e-16
Identity = 123/422 (29.15%), Postives = 203/422 (48.10%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLV-PKQE 67
           KK WH  +LRN+E VWKAEQKHEAE+KK++EL+++++EE+ R+E +   E  G V  K+E
Sbjct: 9   KKSWHPQTLRNVEKVWKAEQKHEAERKKIEELQRELREERAREEMQRYAEDVGAVKKKEE 68

Query: 68  RLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEP----SSSKEAAAPGALFENKP 127
           +LD++Y+     G G   +  + L   P    +    E      SS+    PG++F   P
Sbjct: 69  KLDWMYQ-----GPGGMVNRDEYLLGRPIDKYVFEKMEEREAGCSSETGLLPGSIF--AP 128

Query: 128 HSAN---DSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKR 187
             AN   D   K+  DPL IIR++E+E    V NNP++M  I++ +++   K++    K 
Sbjct: 129 SGANSLLDMASKIREDPLFIIRKKEEEKKREVLNNPVKMKKIKELLQMSLEKKEKKKKKE 188

Query: 188 KRKKHNHSKSKRHKDFSSERDYDSENASPERQRRHDRDKSSKHDHFYSEDRRSKTD--FK 247
           K+KKH   K +      S  + +   A  +++  +     SK   +  + R S  +   +
Sbjct: 189 KKKKHRKHKHRSSSSGGSSSEDEQSQARSQKKMANSFPVLSKVPGYGLQVRDSDRNRGLQ 248

Query: 248 NERVHDRGSKYAAHSTDQS-DRKTSKTSTHGSAERHHDRSKRERDSYASSEKNSRRVGEL 307
                 R  K  + S   S  R  SK ST    ER  DR  R     + S + S+     
Sbjct: 249 GSLGEQRAIKNNSRSRSSSPPRHASKKST--KEERPRDRRSRSPSRRSRSPRPSKPHTSK 308

Query: 308 RSYESNSSETPHESRHKHRRT---INKLSEEERAARLREMQQDAELHDEQRWRRLKKAAE 367
            + +   S +P +  ++ R       KLS EE   + +EM ++A+  +E+R   LK+ A 
Sbjct: 309 VNRKERDSPSPKKEAYQRRHASGYTRKLSAEELERKRQEMMENAKWREEERLNTLKRHAR 368

Query: 368 DDALEAK-QAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKS-EIEANAF 414
           +D  E + +  +     FL   +      E   +S++E+ ++R  +  Q  S  +E N  
Sbjct: 369 EDEREHRLERLDSRSGKFLHRMK-----LESASTSSLEDRVKRNIHSLQRTSVALEKNFM 416

BLAST of Moc10g02820 vs. ExPASy Swiss-Prot
Match: Q4P6I3 (Pre-mRNA-splicing factor CWC25 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=237631 GN=CWC25 PE=3 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 1.6e-09
Identity = 102/395 (25.82%), Postives = 185/395 (46.84%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLVPKQER 67
           KK WH     N E VWK E++   E+KKL+ELR++ ++E+E Q+ + LQE+AG   + +R
Sbjct: 9   KKSWHPLLQVNQERVWKREKEALEERKKLEELRREREQEREMQQLQRLQEEAGGKKRIDR 68

Query: 68  LDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFENKPHSAND 127
           +D++Y +    G GS+++    L        ++   E     +      +     +SA D
Sbjct: 69  VDWMYATPATTGSGSAAEMEDYLLGKKRVDKLLQGEENKEMSKTTQKSLVSLQNANSARD 128

Query: 128 SWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKR----KRKK 187
              K+  DP+L I+Q+EQ A   +  +P ++  ++    ++   +   + +R    KR++
Sbjct: 129 LAAKIREDPMLAIKQQEQAAYEALLRDPTRLRQLKMQAGIDTDTQSKDERRRSKEEKRRR 188

Query: 188 HNHSKSKRHKDFSSERDYDSENASPERQRRHDRDK-------SSKHDHFYSEDRRSKTDF 247
           H+  + +R       R  +S + S    RR DRD+       SS   H   E+RRS    
Sbjct: 189 HDRDEHRRR----GSRHGESRSESRSHSRRDDRDRKSSWNEGSSSRRHDGDEERRSLKRH 248

Query: 248 KNERVHDRGSKYAAHSTDQSDRKTSKTSTHGSAERH--HDRSKRERDSYASSEKNSRRVG 307
           ++++  DR  +Y     D  D + +  S     ++    + S R+  SY+ S   S    
Sbjct: 249 RDDQDRDR-HRYERRLHDADDSRPASRSNRDRRDKDAVDESSSRQSTSYSRSRSRSPPAR 308

Query: 308 ELRSYESNSSETPHESRHKHRRTINKLSEEE-----RAARLREMQQDAELHDEQRWRRLK 367
           + R+ +  SS +            N+  +EE     R  +L+EM+Q+A   ++QR   + 
Sbjct: 309 DYRNDKRLSSSSAFTRPSNGGSARNREEDEERKQQARQLKLQEMEQNARSMEQQRSSYVS 368

Query: 368 KAAEDDALEAKQAAEPSGKNFLDAAQKRMYGAEKG 385
           K   ++A + ++ AE   K  L  A+ + +G  KG
Sbjct: 369 KINAEEAEQERREAELRQK--LIDARSKGHGDGKG 396

BLAST of Moc10g02820 vs. ExPASy Swiss-Prot
Match: Q5B0I1 (Pre-mRNA-splicing factor cwc25 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=cwc25 PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.0e-07
Identity = 117/420 (27.86%), Postives = 194/420 (46.19%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLVPKQER 67
           KK WH   LRN E VW  E++   E+K++D+L+++  EE++ QE + LQE AG   + +R
Sbjct: 8   KKSWHPSLLRNQERVWAEEKRALEERKRIDQLKRERDEERQIQELQRLQESAGGTRQLQR 67

Query: 68  LDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKE---AAAPGALFENKP-- 127
           +D++Y+   +     + +    L        I+   + S   E   A  PGA     P  
Sbjct: 68  VDWMYQEPSSASGHYAEEMEGYLLGKRRIDGILLKNDESKKLEKGAATGPGAAAGLPPVV 127

Query: 128 HSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVK---VEKHKEKNPDDKR 187
           H+  D+  K+ +DPLL IR+REQ A           AM++++V+    E+ +E++ + +R
Sbjct: 128 HNTRDTMAKVMADPLLEIRKREQAAYE---------AMVKESVRRREREQGRERHQERER 187

Query: 188 KRKKHNH---SKSKRHKDFSSERDYDSENASPERQRRHDRDKSSKHDHFYSEDRRSKTDF 247
           +R  H H   S +  H+     R   S + SP  ++   R +  +HD     DR  + + 
Sbjct: 188 ER-GHRHRRDSGNDSHRSHRHRRRSRSRSRSPRVEKDDRRHRDREHDRRSHRDRDGEREN 247

Query: 248 KNERV-------HDRGSKYAAHSTDQSDRKTSKTSTHGSAERHHDRSKRERDSYASSEKN 307
           + ERV       HDR  +Y        DR   ++++     R  DR  R++D     ++N
Sbjct: 248 RVERVYHSSRRDHDRDERY--------DRAARRSASPRPDRRRDDRRDRDQDQDYRRDRN 307

Query: 308 SRRV-GELRSYESNSSETPHESRHKHRRTINKLS-------------EEERAARLREMQQ 367
           SR    + RS     ++     R++ R + ++               EE+R  RL EMQ 
Sbjct: 308 SRSSRRDARSSRDRDTDAFGRDRNRDRGSHSRNGNADANADQHAQELEEQRKRRLAEMQA 367

Query: 368 DAELHDEQRWRRLKKAAEDDALEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRR 396
           +A+  D +  RR ++ AE  ALE KQ  E   K   D  Q       +    +++E IRR
Sbjct: 368 NAQ--DMEATRR-QRVAEVTALEEKQ-REEDDKRRSDRGQFMSGLHRQLQEDSLDERIRR 405

BLAST of Moc10g02820 vs. ExPASy Swiss-Prot
Match: Q4IRI9 (Pre-mRNA-splicing factor CWC25 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=CWC25 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.7e-06
Identity = 104/398 (26.13%), Postives = 175/398 (43.97%), Query Frame = 0

Query: 8   KKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAGLVPKQER 67
           KK +H    RN + VW  EQK  AE+K+  +   +I+EE+ ++E +   E AG   K +R
Sbjct: 9   KKSFHPALRRNQQAVWDEEQKALAERKRTQQRLDEIKEERAKEEVQRQLEAAGGKKKVDR 68

Query: 68  LDFLYESGLAVGKGSSSD------GFKSLETLPSTSAIVATTEPSSSKEAAAPGALFENK 127
           ++++Y+       G++ +      G + ++ L     I  T      K+A     +    
Sbjct: 69  VEWMYQGPNDGQTGTTEETEAYLLGKRRIDNL-----IKGTEHKKLEKDAGTESFMALQN 128

Query: 128 PHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRKR 187
            ++A D+  K+  DPLL I+++EQ A   + N+P +    R+ +   +  +++  DK +R
Sbjct: 129 ANTARDTAAKIREDPLLAIKRQEQAAYEAMMNDPAR----RRQLLASRGIDEDKKDKSRR 188

Query: 188 KKHNHSKSKRHKDFSSERDYDSENASPERQRRHDRDKSSKHDHFYSEDRRSKTDFKNERV 247
           K+  H +  RH      R  D E+    R+R + R +S +      EDR  +   + +  
Sbjct: 189 KEDRHRRRHRH------RSADGEDRHHRRRRSYSRSQSPRRRDGSREDRHRR---RRDDS 248

Query: 248 HDRGSKYAAHSTDQSDRKTSKTSTHGSAERHHDRSKRERDSYASSEKNSRRVGELRSYES 307
            DR S+     +D+  R+     + G   R HD  +R  D      +++RR    R    
Sbjct: 249 RDRHSRRRRDGSDEEGRRDRDNRSGGDRHR-HDGDRRHSDEPRDERRDNRRDERPRDNNR 308

Query: 308 NSSET--PHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLK--KAAEDDAL 367
             S    P + R +H    +K  EEER  +L  MQ  A   D  R  RL   +  E  A 
Sbjct: 309 QYSRRSYPSDRRPRHNEEDDKAKEEERQRKLAAMQSAATELDVDRQERLADLEKREQAAR 368

Query: 368 EAKQAAEPSG--KNFLD---------AAQKRMYGAEKG 385
           EA   A   G  + F++            +RM GA +G
Sbjct: 369 EADNKARERGGDRGFVNKLHQQAEHKGLAERMGGARRG 387

BLAST of Moc10g02820 vs. ExPASy TrEMBL
Match: A0A6J1CHZ1 (pre-mRNA-splicing factor CWC25 homolog OS=Momordica charantia OX=3673 GN=LOC111011671 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.1e-213
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120
           LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120

Query: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180
           KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK
Sbjct: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180

Query: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRRHDRDKSSKHDHFYSEDRRSKTDFKNER 240
           RKKHNHSKSKRHKDFSSERDYDSENASPERQRRHDRDKSSKHDHFYSEDRRSKTDFKNER
Sbjct: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRRHDRDKSSKHDHFYSEDRRSKTDFKNER 240

Query: 241 VHDRGSKYAAHSTDQSDRKTSKTSTHGSAERHHDRSKRERDSYASSEKNSRRVGELRSYE 300
           VHDRGSKYAAHSTDQSDRKTSKTSTHGSAERHHDRSKRERDSYASSEKNSRRVGELRSYE
Sbjct: 241 VHDRGSKYAAHSTDQSDRKTSKTSTHGSAERHHDRSKRERDSYASSEKNSRRVGELRSYE 300

Query: 301 SNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDALEAK 360
           SNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDALEAK
Sbjct: 301 SNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDALEAK 360

Query: 361 QAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 414
           QAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR
Sbjct: 361 QAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 413

BLAST of Moc10g02820 vs. ExPASy TrEMBL
Match: A0A5A7TSZ0 (Pre-mRNA-splicing factor CWC25-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00360 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.9e-174
Identity = 358/419 (85.44%), Postives = 384/419 (91.65%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120
           LVPKQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKEAAAPGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHK+KNPDDKR+
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRR-HDRDKSSKHD-HFYSEDRRSKTDFKN 240
           RKKH HSKSKRHKD S ERDYDSE+ASPERQRR HD DKSS+HD H +SEDRRSK D KN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240

Query: 241 ERVHDRGSKYAAH-STDQSDRKTSKTSTHGS-AERHHDRSK--RERDSYASSEKNSRRVG 300
           ER  DRGSKYAA  S DQSDRKTSK++   S A+RHHDRSK  R+RDS+A+++K+SRRVG
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVG 300

Query: 301 ELRSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAED 360
           E R YES++SETP+ESRH+HRR   KLSEEERAARLREMQQDAELH+EQRW+RLKKAAED
Sbjct: 301 EPRYYESHASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360

Query: 361 DALEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 414
           DALEAKQ A PS +NFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR
Sbjct: 361 DALEAKQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 419

BLAST of Moc10g02820 vs. ExPASy TrEMBL
Match: A0A1S3BY15 (pre-mRNA-splicing factor CWC25 homolog OS=Cucumis melo OX=3656 GN=LOC103494454 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.9e-174
Identity = 358/419 (85.44%), Postives = 384/419 (91.65%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120
           LVPKQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKEAAAPGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAAPGALFED 120

Query: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHK+KNPDDKR+
Sbjct: 121 KPHSANDAWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRR-HDRDKSSKHD-HFYSEDRRSKTDFKN 240
           RKKH HSKSKRHKD S ERDYDSE+ASPERQRR HD DKSS+HD H +SEDRRSK D KN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDASPERQRRKHDHDKSSRHDGHSHSEDRRSKADSKN 240

Query: 241 ERVHDRGSKYAAH-STDQSDRKTSKTSTHGS-AERHHDRSK--RERDSYASSEKNSRRVG 300
           ER  DRGSKYAA  S DQSDRKTSK++   S A+RHHDRSK  R+RDS+A+++K+SRRVG
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTSKSNPRDSAADRHHDRSKRDRDRDSHANNDKDSRRVG 300

Query: 301 ELRSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAED 360
           E R YES++SETP+ESRH+HRR   KLSEEERAARLREMQQDAELH+EQRW+RLKKAAED
Sbjct: 301 EPRYYESHASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRWKRLKKAAED 360

Query: 361 DALEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 414
           DALEAKQ A PS +NFLD AQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR
Sbjct: 361 DALEAKQNAVPSSRNFLDTAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 419

BLAST of Moc10g02820 vs. ExPASy TrEMBL
Match: A0A0A0KU62 (Cir_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G025080 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.6e-172
Identity = 352/417 (84.41%), Postives = 381/417 (91.37%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQILEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALFEN 120
           LVPKQERLDFLYESGLAVGK SSSDGFKSLETLPS+S   A TEPSSSKEAA PGALFE+
Sbjct: 61  LVPKQERLDFLYESGLAVGKASSSDGFKSLETLPSSSTAAAATEPSSSKEAAVPGALFED 120

Query: 121 KPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKRK 180
           KPHSAND+WRKLHSDPLLIIRQREQ+ALARVKNNPIQMAMIRKTV+VEKHK+KNPDDKR+
Sbjct: 121 KPHSANDTWRKLHSDPLLIIRQREQQALARVKNNPIQMAMIRKTVEVEKHKDKNPDDKRE 180

Query: 181 RKKHNHSKSKRHKDFSSERDYDSENASPERQRR-HDRDKSSKHD-HFYSEDRRSKTDFKN 240
           RKKH HSKSKRHKD S ERDYDSE+ SPERQRR HD DKSS+HD H +SEDRRSK + KN
Sbjct: 181 RKKHRHSKSKRHKDSSPERDYDSEDVSPERQRRKHDHDKSSRHDGHSHSEDRRSKAETKN 240

Query: 241 ERVHDRGSKYAAH-STDQSDRKTSKTSTHGS-AERHHDRSKRERDSYASSEKNSRRVGEL 300
           ER  DRGSKYAA  S DQSDRKT K++ H S A+R+HDRSKR+RDSYA+++++SRRVGEL
Sbjct: 241 ERDRDRGSKYAARTSYDQSDRKTFKSNPHDSAADRYHDRSKRDRDSYATNDRDSRRVGEL 300

Query: 301 RSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAAEDDA 360
           R YESN+SETP+ESRH+HRR   KLSEEERAARLREMQQDAELH+EQR++RLKKA EDDA
Sbjct: 301 RYYESNASETPNESRHRHRRPTTKLSEEERAARLREMQQDAELHEEQRFKRLKKADEDDA 360

Query: 361 LEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFRR 414
           LEAKQ A PS +NFLD AQKRMY AEKGGSSTIEESIRRRTYYSQGKS+IEANAFRR
Sbjct: 361 LEAKQNAVPSSRNFLDMAQKRMYSAEKGGSSTIEESIRRRTYYSQGKSQIEANAFRR 417

BLAST of Moc10g02820 vs. ExPASy TrEMBL
Match: A0A6J1E963 (LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita moschata OX=3662 GN=LOC111430542 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 1.0e-163
Identity = 344/421 (81.71%), Postives = 364/421 (86.46%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQI EEKERQEFRLLQEQAG
Sbjct: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIHEEKERQEFRLLQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLP-STSAIVATTEPSSSKEAAAPGALFE 120
           LVPKQERLDFLYESGLAVGKGS+SDGFKSLETLP +TS   A  EPSSSKEAAAPGALFE
Sbjct: 61  LVPKQERLDFLYESGLAVGKGSNSDGFKSLETLPTTTSTAAAAPEPSSSKEAAAPGALFE 120

Query: 121 NKPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKR 180
           +KPHSAND+WRKLHSDPLLIIRQREQ ALARVKNNPIQMAMIRKTV+VEKHK KNPDD+ 
Sbjct: 121 DKPHSANDAWRKLHSDPLLIIRQREQXALARVKNNPIQMAMIRKTVEVEKHKAKNPDDEG 180

Query: 181 KRKKHNHSKSKRHKDFSSERDYDSENASPERQRR----HDRDKSSKHD-HFYSEDRRSKT 240
           +RKKH HSKSKR KD    R+YDSE+ASPERQRR    HDRDKSSK D   YSEDRRSKT
Sbjct: 181 ERKKHCHSKSKRRKDSPPGREYDSESASPERQRRKRRNHDRDKSSKRDVRSYSEDRRSKT 240

Query: 241 DFKNERVHDRGSKYAAHSTDQSDRKTSKTSTHG--SAERHHDRSKRERDSYASSEKNSRR 300
           D+ NER  D GSKY     DQSDRKT K++ H   + ERHHD SKR RDSY  +EK+SRR
Sbjct: 241 DYMNERDRDSGSKY-----DQSDRKTPKSNPHDYYAEERHHDSSKRYRDSYTHNEKDSRR 300

Query: 301 VGELRSYESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAELHDEQRWRRLKKAA 360
           VGE R+YE N+S TP E RHKHRR   KLSEEERAARLREMQQDAE H+EQRWRRLKKAA
Sbjct: 301 VGESRTYEPNASNTPFEPRHKHRRPATKLSEEERAARLREMQQDAEFHEEQRWRRLKKAA 360

Query: 361 EDDALEAKQAAEPSGKNFLDAAQKRMYGAEKGGSSTIEESIRRRTYYSQGKSEIEANAFR 414
           EDDALEA+Q A PS +NFLD AQKRMY AEKGGSSTIEESIRRRTYYSQGKSEIEANAFR
Sbjct: 361 EDDALEAEQNAVPSSRNFLDTAQKRMYSAEKGGSSTIEESIRRRTYYSQGKSEIEANAFR 416

BLAST of Moc10g02820 vs. TAIR 10
Match: AT2G44200.1 (CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor )

HSP 1 Score: 305.8 bits (782), Expect = 5.1e-83
Identity = 229/494 (46.36%), Postives = 283/494 (57.29%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           M +KFLNKKGWHTGSLRNIE VWKAEQK EAEQKKL+ELR QI +E+ER EFR LQEQAG
Sbjct: 1   MGMKFLNKKGWHTGSLRNIETVWKAEQKQEAEQKKLEELRLQILQERERSEFRALQEQAG 60

Query: 61  LVPKQERLDFLYESGLAVGKGSSSDGFKSLETLPSTSA-IVATTEPSSSKEAAAPGALFE 120
           L+P+QERL+FLY+SGLAVGKGS+S    S +      A   A +  S   + +APGALFE
Sbjct: 61  LIPRQERLEFLYDSGLAVGKGSASGSGVSFQKEEQPLAKSEAGSSASEKPDQSAPGALFE 120

Query: 121 NKPHSANDSWRKLHSDPLLIIRQREQEALARVKNNPIQMAMIRKTVKVEKHKEKNPDDKR 180
            K  SANDSWRKLHSDPLL+IRQREQEALA++KNNP++MA+IRK+V+ EK K K+ D K 
Sbjct: 121 EKAQSANDSWRKLHSDPLLLIRQREQEALAKIKNNPVKMALIRKSVE-EKGKGKDGDTKE 180

Query: 181 KRKKHNHSKSKRHKDFSSERDYDSENASPER---QRRHDRDKSSKHDHFYS--------- 240
            +KKH     K  K  SS +  DSE  S E    ++ H +  S  HD  Y          
Sbjct: 181 HKKKHKRKSGKHQKQSSSRQRSDSEEDSGEENNGRKSHHQKTSGTHDRHYERPRSDLEDE 240

Query: 241 -----------EDRRSKTDFKNERVHDRGSKYAAHSTDQSDRKTSKTSTHGSAERHH--- 300
                      E RRS+ D  ++R     + Y    ++  D    + S     ER     
Sbjct: 241 SKGRESRDRHYEKRRSELDDGHKRRERHDTHYERRRSEMDDESKRRESRDNHYERRRSDL 300

Query: 301 -DRSKRER--------------DSYASSEKNSRR-------------------------- 360
            D SKR                D Y   E   +R                          
Sbjct: 301 DDESKRRESHDKHFERQRSDLDDEYKRRESQDKRRRSDIDDEPKRRDARPNEKYRNRSPK 360

Query: 361 ----VGELRSY---------ESNSSETPHESRHKHRRTINKLSEEERAARLREMQQDAEL 414
                  L+SY         E   S  P+E +++ R+  +KLSEEERAARL++MQ DAE+
Sbjct: 361 GGVERENLKSYGQEDKKRKAEDLDSGKPNEYQNRRRKGGSKLSEEERAARLKQMQMDAEV 420

BLAST of Moc10g02820 vs. TAIR 10
Match: AT2G44195.1 (CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor )

HSP 1 Score: 142.5 bits (358), Expect = 7.5e-34
Identity = 84/149 (56.38%), Postives = 101/149 (67.79%), Query Frame = 0

Query: 1   MALKFLNKKGWHTGSLRNIENVWKAEQKHEAEQKKLDELRKQIQEEKERQEFRLLQEQAG 60
           MALKFLNKKGWHTGS+RN+E VWKAEQKHEAEQ+K++          ER EFR +QEQAG
Sbjct: 1   MALKFLNKKGWHTGSIRNVEKVWKAEQKHEAEQEKIE----------ERSEFRAIQEQAG 60

Query: 61  LVPKQ-ERLDFLYESGLAVGKGSSSDGFKSLETLPSTSAIVATTEPSSSKEAAAPGALF- 120
           LVP++ ERL+FLY+  LAV K          + + S+   V          A  PGALF 
Sbjct: 61  LVPRRPERLEFLYDPELAVAK----------QNVSSSRHGVLFQNQDQRARATIPGALFD 120

Query: 121 -ENKPHSANDSWRKLHSDPLLIIRQREQE 147
            ++K HSANDSWRK HSDPLL+IRQ+EQE
Sbjct: 121 DDDKRHSANDSWRKFHSDPLLLIRQKEQE 129

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141219.12.2e-213100.00pre-mRNA-splicing factor CWC25 homolog [Momordica charantia][more]
XP_038896474.18.3e-17686.57pre-mRNA-splicing factor CWC25 homolog [Benincasa hispida][more]
XP_008453851.15.9e-17485.44PREDICTED: pre-mRNA-splicing factor CWC25 homolog [Cucumis melo] >KAA0044681.1 p... [more]
XP_004146897.13.3e-17284.41pre-mRNA-splicing factor CWC25 homolog [Cucumis sativus] >KGN53175.1 hypothetica... [more]
XP_023551879.11.5e-16482.25LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog, partial [Cucurbita ... [more]
Match NameE-valueIdentityDescription
Q9NXE82.8e-1731.34Pre-mRNA-splicing factor CWC25 homolog OS=Homo sapiens OX=9606 GN=CWC25 PE=1 SV=... [more]
Q9DBF71.8e-1629.15Pre-mRNA-splicing factor CWC25 homolog OS=Mus musculus OX=10090 GN=Cwc25 PE=2 SV... [more]
Q4P6I31.6e-0925.82Pre-mRNA-splicing factor CWC25 OS=Ustilago maydis (strain 521 / FGSC 9021) OX=23... [more]
Q5B0I12.0e-0727.86Pre-mRNA-splicing factor cwc25 OS=Emericella nidulans (strain FGSC A4 / ATCC 381... [more]
Q4IRI91.7e-0626.13Pre-mRNA-splicing factor CWC25 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 12... [more]
Match NameE-valueIdentityDescription
A0A6J1CHZ11.1e-213100.00pre-mRNA-splicing factor CWC25 homolog OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5A7TSZ02.9e-17485.44Pre-mRNA-splicing factor CWC25-like protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BY152.9e-17485.44pre-mRNA-splicing factor CWC25 homolog OS=Cucumis melo OX=3656 GN=LOC103494454 P... [more]
A0A0A0KU621.6e-17284.41Cir_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G025080 PE=3 ... [more]
A0A6J1E9631.0e-16381.71LOW QUALITY PROTEIN: pre-mRNA-splicing factor CWC25 homolog OS=Cucurbita moschat... [more]
Match NameE-valueIdentityDescription
AT2G44200.15.1e-8346.36CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor [more]
AT2G44195.17.5e-3456.38CBF1-interacting co-repressor CIR, N-terminal;Pre-mRNA splicing factor [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 22..49
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 302..324
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 176..190
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 101..129
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 150..324
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 191..295
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 380..413
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 396..413
NoneNo IPR availablePANTHERPTHR16196CELL CYCLE CONTROL PROTEIN CWF25coord: 1..413
IPR019339CBF1-interacting co-repressor CIR, N-terminal domainSMARTSM01083Cir_N_3coord: 10..46
e-value: 3.9E-13
score: 59.6
IPR019339CBF1-interacting co-repressor CIR, N-terminal domainPFAMPF10197Cir_Ncoord: 11..46
e-value: 7.9E-15
score: 54.8
IPR022209Pre-mRNA splicing factorPFAMPF12542CWC25coord: 64..155
e-value: 2.2E-11
score: 44.5

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g02820.1Moc10g02820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000398 mRNA splicing, via spliceosome
cellular_component GO:0005684 U2-type spliceosomal complex