Moc02g04540 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g04540
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Nuclear transcription factor Y subunit B-3
Location	chr2: 3368241 .. 3368861 (-)
RNA-Seq Expression	Moc02g04540
Synteny	Moc02g04540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGATTCGGATAACGAATCGGGAGGACACAACAGCAACGCGAACAGCGAGCTATCGGCGAAAGAGCAGGACAGATTCCTGCCAATAGCGAACGTCAGCAGGATCATGAAGAAAGCGCTACCGGCGAACGCGAAGATCTCGAAAGACGCGAAAGAAACGGTACAGGAGTGCGTATCGGAGTTCATCAGCTTCATCACCGGAGAGGCATCGGACAAGTGCCAGAGAGAGAAGAGGAAGACGATCAACGGCGACGATTTGTTGTGGGCCATGACGACGCTAGGTTTCGAAGAGTATGTGGAACCGCTCAAGACTTATCTGCAGAAGTACAGGGAAATGGAAGGGGAGAAGAGCACTATGGGGAGACAGGGGGAGAAAGACGGTGGTGGTGGTGGGCCCGGCGGCAGTGGGGCTGGTGGCGGTGGTGGTGGAGGAGTCAACTCTGGTACTGCTGGTGGTGCTGGTGGCGGCGGCTTTAACGGAGTGGGTGGAATGTATGGTGGGATGATGATGATGGAGCATCATCAGGGGGGCGTCTACGGTGGCTCTGGTGGATTTCACCACATGGGCATCGGCGGCGGGAAGGGGGGAAGCGGTGGCTCCGGCACGGGGCACCGATAG

mRNA sequence

ATGGCGGATTCGGATAACGAATCGGGAGGACACAACAGCAACGCGAACAGCGAGCTATCGGCGAAAGAGCAGGACAGATTCCTGCCAATAGCGAACGTCAGCAGGATCATGAAGAAAGCGCTACCGGCGAACGCGAAGATCTCGAAAGACGCGAAAGAAACGGTACAGGAGTGCGTATCGGAGTTCATCAGCTTCATCACCGGAGAGGCATCGGACAAGTGCCAGAGAGAGAAGAGGAAGACGATCAACGGCGACGATTTGTTGTGGGCCATGACGACGCTAGGTTTCGAAGAGTATGTGGAACCGCTCAAGACTTATCTGCAGAAGTACAGGGAAATGGAAGGGGAGAAGAGCACTATGGGGAGACAGGGGGAGAAAGACGGTGGTGGTGGTGGGCCCGGCGGCAGTGGGGCTGGTGGCGGTGGTGGTGGAGGAGTCAACTCTGGTACTGCTGGTGGTGCTGGTGGCGGCGGCTTTAACGGAGTGGGTGGAATGTATGGTGGGATGATGATGATGGAGCATCATCAGGGGGGCGTCTACGGTGGCTCTGGTGGATTTCACCACATGGGCATCGGCGGCGGGAAGGGGGGAAGCGGTGGCTCCGGCACGGGGCACCGATAG

Coding sequence (CDS)

ATGGCGGATTCGGATAACGAATCGGGAGGACACAACAGCAACGCGAACAGCGAGCTATCGGCGAAAGAGCAGGACAGATTCCTGCCAATAGCGAACGTCAGCAGGATCATGAAGAAAGCGCTACCGGCGAACGCGAAGATCTCGAAAGACGCGAAAGAAACGGTACAGGAGTGCGTATCGGAGTTCATCAGCTTCATCACCGGAGAGGCATCGGACAAGTGCCAGAGAGAGAAGAGGAAGACGATCAACGGCGACGATTTGTTGTGGGCCATGACGACGCTAGGTTTCGAAGAGTATGTGGAACCGCTCAAGACTTATCTGCAGAAGTACAGGGAAATGGAAGGGGAGAAGAGCACTATGGGGAGACAGGGGGAGAAAGACGGTGGTGGTGGTGGGCCCGGCGGCAGTGGGGCTGGTGGCGGTGGTGGTGGAGGAGTCAACTCTGGTACTGCTGGTGGTGCTGGTGGCGGCGGCTTTAACGGAGTGGGTGGAATGTATGGTGGGATGATGATGATGGAGCATCATCAGGGGGGCGTCTACGGTGGCTCTGGTGGATTTCACCACATGGGCATCGGCGGCGGGAAGGGGGGAAGCGGTGGCTCCGGCACGGGGCACCGATAG

Protein sequence

MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTMGRQGEKDGGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR

Homology

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BLAST of Moc02g04540 vs. NCBI nr
Match: XP_008464158.1 (PREDICTED: nuclear transcription factor Y subunit B-3 [Cucumis melo] >XP_008464159.1 PREDICTED: nuclear transcription factor Y subunit B-3 [Cucumis melo] >KAA0060811.1 nuclear transcription factor Y subunit B-3 [Cucumis melo var. makuwa] >TYK08821.1 nuclear transcription factor Y subunit B-3 [Cucumis melo var. makuwa])

HSP 1 Score: 330.5 bits (846), Expect = 1.0e-86
Identity = 191/207 (92.27%), Postives = 194/207 (93.72%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVN-SGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGV 180
           GRQGEKDGGGGGPGGS      GGGVN SGTA G  GGG+NGVGGMYGG+MMM HHQGGV
Sbjct: 121 GRQGEKDGGGGGPGGS------GGGVNSSGTAAGGAGGGYNGVGGMYGGVMMMGHHQGGV 180

Query: 181 YGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           YGG+ GFHHMGIGGGKGGSG SGTGHR
Sbjct: 181 YGGA-GFHHMGIGGGKGGSGASGTGHR 200

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BLAST of Moc02g04540 vs. NCBI nr
Match: XP_022971106.1 (nuclear transcription factor Y subunit B-3-like [Cucurbita maxima])

HSP 1 Score: 329.3 bits (843), Expect = 2.3e-86
Identity = 196/211 (92.89%), Postives = 199/211 (94.31%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLK+YLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKSYLQKYREMEGEKSTM 120

Query: 121 -GRQGEKD--GGGGGPGGSGA--GGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHH 180
            G +GEKD  GGGGGPGGSGA  GGGGGGGVN GT GG  GGGFNGVGGMYGGMMMM HH
Sbjct: 121 GGGKGEKDGGGGGGGPGGSGANVGGGGGGGVNPGTGGGVSGGGFNGVGGMYGGMMMMVHH 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           QGGVY GSGGFHHMGIGGGKGG+GGSGTGHR
Sbjct: 181 QGGVY-GSGGFHHMGIGGGKGGNGGSGTGHR 210

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BLAST of Moc02g04540 vs. NCBI nr
Match: XP_022947534.1 (nuclear transcription factor Y subunit B-3-like [Cucurbita moschata] >KAG6604918.1 Nuclear transcription factor Y subunit B-3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 326.2 bits (835), Expect = 2.0e-85
Identity = 195/211 (92.42%), Postives = 198/211 (93.84%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLK+YLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKSYLQKYREMEGEKSTM 120

Query: 121 -GRQGEKD--GGGGGPGGSGA--GGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHH 180
            G +GEKD  GGGGGPGGSGA  GGGGGGGVN GT GG  GGGFNGVGGMYGGMMMM HH
Sbjct: 121 GGGKGEKDGGGGGGGPGGSGANVGGGGGGGVNPGTGGGVSGGGFNGVGGMYGGMMMMGHH 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           QGGVY GSGGFH MGIGGGKGG+GGSGTGHR
Sbjct: 181 QGGVY-GSGGFHQMGIGGGKGGNGGSGTGHR 210

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BLAST of Moc02g04540 vs. NCBI nr
Match: XP_004139999.1 (nuclear transcription factor Y subunit B-3 [Cucumis sativus] >KGN46611.1 hypothetical protein Csa_005340 [Cucumis sativus])

HSP 1 Score: 323.9 bits (829), Expect = 9.8e-85
Identity = 190/208 (91.35%), Postives = 193/208 (92.79%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVN-SGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGV 180
           GRQGEKDGGGGGPGGS      GGGVN SG A G  GGG+NGVGGMYGG+MMM HHQGGV
Sbjct: 121 GRQGEKDGGGGGPGGS------GGGVNSSGAAAGGAGGGYNGVGGMYGGVMMMGHHQGGV 180

Query: 181 YGGSGGFHHMGIGGGKGGSGG-SGTGHR 207
           YGG+ GFHHMGIG GKGGSGG SGTGHR
Sbjct: 181 YGGA-GFHHMGIGSGKGGSGGASGTGHR 201

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BLAST of Moc02g04540 vs. NCBI nr
Match: XP_023532503.1 (nuclear transcription factor Y subunit B-3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 323.6 bits (828), Expect = 1.3e-84
Identity = 193/209 (92.34%), Postives = 196/209 (93.78%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLK+YLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKSYLQKYREMEGEKSTM 120

Query: 121 -GRQGEKD--GGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHHQG 180
            G +GEKD  GGGGGPGGSGA   GGGGVN GT GG  GGGFNGVGGMYGGMMMM HHQG
Sbjct: 121 GGGKGEKDGGGGGGGPGGSGA-NVGGGGVNPGTGGGVSGGGFNGVGGMYGGMMMMGHHQG 180

Query: 181 GVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           GVY GSGGFHHMGIGGGKGG+GGSGTGHR
Sbjct: 181 GVY-GSGGFHHMGIGGGKGGNGGSGTGHR 207

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BLAST of Moc02g04540 vs. ExPASy Swiss-Prot
Match: O23310 (Nuclear transcription factor Y subunit B-3 OS=Arabidopsis thaliana OX=3702 GN=NFYB3 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 7.1e-54
Identity = 124/151 (82.12%), Postives = 135/151 (89.40%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDN+SGGH    N+  S +EQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNDSGGHKDGGNA--STREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEK-ST 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFE+YVEPLK YLQKYRE+EGEK +T
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQKYREVEGEKTTT 120

Query: 121 MGRQGEKDGGGGGPGGSGAGGGG----GGGV 147
            GRQG+K+GGGGG GG+G+G GG    GGG+
Sbjct: 121 AGRQGDKEGGGGG-GGAGSGSGGAPMYGGGM 148

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BLAST of Moc02g04540 vs. ExPASy Swiss-Prot
Match: Q69J40 (Nuclear transcription factor Y subunit B-10 OS=Oryza sativa subsp. japonica OX=39947 GN=NFYB10 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.0e-44
Identity = 136/220 (61.82%), Postives = 148/220 (67.27%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           M DSDNESGG  SNA    SA+EQDRFLPIANVSRIMK+ALPANAKISKDAKETVQECVS
Sbjct: 1   MPDSDNESGG-PSNAGEYASAREQDRFLPIANVSRIMKRALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFE+Y++PLK YL K+RE+EGEK+  
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEDYIDPLKLYLHKFRELEGEKAI- 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGMYGG-----MMMMEHH 180
                   G  G GG GA   GG G  SG+         NG  GMYGG     MMM +  
Sbjct: 121 --------GAAGSGGGGAASSGGSGSGSGSHHHQDASRNNGGYGMYGGGGGMIMMMGQPM 180

Query: 181 QGGVYGGSGGF---------HHMGIGGGKG--GSGGSGTG 205
            G     S G+         HH  + GGKG  G GG G G
Sbjct: 181 YGSPPASSAGYAQPPPPHHHHHQMVMGGKGAYGHGGGGGG 210

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BLAST of Moc02g04540 vs. ExPASy Swiss-Prot
Match: Q9FGJ3 (Nuclear transcription factor Y subunit B-2 OS=Arabidopsis thaliana OX=3702 GN=NFYB2 PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.0e-44
Identity = 124/207 (59.90%), Postives = 141/207 (68.12%), Query Frame = 0


Query: 1   MADSDNESG----GHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQ 60
           M DSD +SG    G+N N  S LS +EQDRFLPIANVSRIMKKALPANAKISKDAKET+Q
Sbjct: 1   MGDSDRDSGGGQNGNNQNGQSSLSPREQDRFLPIANVSRIMKKALPANAKISKDAKETMQ 60

Query: 61  ECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGE 120
           ECVSEFISF+TGEASDKCQ+EKRKTINGDDLLWAMTTLGFE+YVEPLK YLQ++RE+EGE
Sbjct: 61  ECVSEFISFVTGEASDKCQKEKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQRFREIEGE 120

Query: 121 KSTMGRQGEKDGGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGM-YGGMMMMEHH 180
           ++ +GR                  GG  G +   A G GGG + G GGM Y       H 
Sbjct: 121 RTGLGRPQT---------------GGEVGEHQRDAVGDGGGFYGGGGGMQYHQHHQFLHQ 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSG 203
           Q  +YG +GG      G   GG   SG
Sbjct: 181 QNHMYGATGG------GSDSGGGAASG 186

[Close]

BLAST of Moc02g04540 vs. ExPASy Swiss-Prot
Match: Q75IZ7 (Nuclear transcription factor Y subunit B-8 OS=Oryza sativa subsp. japonica OX=39947 GN=NFYB8 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 1.5e-40
Identity = 129/215 (60.00%), Postives = 143/215 (66.51%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSA-KEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECV 60
           M DSDN+SGG ++ A  ELS+ +EQDRFLPIANVSRIMKKALPANAKISKDAKETVQECV
Sbjct: 1   MPDSDNDSGGPSNYAGGELSSPREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECV 60

Query: 61  SEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKST 120
           SEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFE+YV+PLK YL K+RE+EGE++ 
Sbjct: 61  SEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEDYVDPLKHYLHKFREIEGERAA 120

Query: 121 MGRQGE-KDGGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGG--MYGG-----MMM 180
               G         P          GG     A GAG GG   + G  MYG         
Sbjct: 121 ASTTGAGTSAASTTPPQQQHTANAAGGYAGYAAPGAGPGGMMMMMGQPMYGSPPPPPQQQ 180

Query: 181 MEHHQGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
            + H     GG GGF H   GGG G S  SG G +
Sbjct: 181 QQQHHHMAMGGRGGFGHHPGGGGGGSSSSSGHGRQ 215

[Close]

BLAST of Moc02g04540 vs. ExPASy Swiss-Prot
Match: Q0J7P4 (Nuclear transcription factor Y subunit B-11 OS=Oryza sativa subsp. japonica OX=39947 GN=HD5 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 6.7e-36
Identity = 113/194 (58.25%), Postives = 131/194 (67.53%), Query Frame = 0


Query: 11  HNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISFITGEA 70
           +      +  AKEQDRFLPIANVSRIMK++LPANAKISK++KETVQECVSEFISF+TGEA
Sbjct: 46  YGGGGGGDSPAKEQDRFLPIANVSRIMKRSLPANAKISKESKETVQECVSEFISFVTGEA 105

Query: 71  SDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTMGRQGEKDGGG 130
           SDKCQREKRKTINGDDLLWAMTTLGFE YV PLK+YL +YRE EGEK+ +          
Sbjct: 106 SDKCQREKRKTINGDDLLWAMTTLGFEAYVGPLKSYLNRYREAEGEKADV---------L 165

Query: 131 GGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEH-HQGGVYGGSGGFHHM 190
           GG GG+ A   G GG   G  GGA G   +G   + GG+    H HQ    GG  G    
Sbjct: 166 GGAGGAAAARHGEGGCCGGGGGGADGVVIDGHYPLAGGLSHSHHGHQQQDGGGDVGLMMG 225

Query: 191 GIGGGKGGSGGSGT 204
           G   G G + G+G+
Sbjct: 226 GGDAGVGYNAGAGS 230

[Close]

BLAST of Moc02g04540 vs. ExPASy TrEMBL
Match: A0A1S3CMC5 (nuclear transcription factor Y subunit B-3 OS=Cucumis melo OX=3656 GN=LOC103502105 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 5.1e-87
Identity = 191/207 (92.27%), Postives = 194/207 (93.72%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVN-SGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGV 180
           GRQGEKDGGGGGPGGS      GGGVN SGTA G  GGG+NGVGGMYGG+MMM HHQGGV
Sbjct: 121 GRQGEKDGGGGGPGGS------GGGVNSSGTAAGGAGGGYNGVGGMYGGVMMMGHHQGGV 180

Query: 181 YGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           YGG+ GFHHMGIGGGKGGSG SGTGHR
Sbjct: 181 YGGA-GFHHMGIGGGKGGSGASGTGHR 200

[Close]

BLAST of Moc02g04540 vs. ExPASy TrEMBL
Match: A0A5A7V4W8 (Nuclear transcription factor Y subunit B-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00280 PE=3 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 5.1e-87
Identity = 191/207 (92.27%), Postives = 194/207 (93.72%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVN-SGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGV 180
           GRQGEKDGGGGGPGGS      GGGVN SGTA G  GGG+NGVGGMYGG+MMM HHQGGV
Sbjct: 121 GRQGEKDGGGGGPGGS------GGGVNSSGTAAGGAGGGYNGVGGMYGGVMMMGHHQGGV 180

Query: 181 YGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           YGG+ GFHHMGIGGGKGGSG SGTGHR
Sbjct: 181 YGGA-GFHHMGIGGGKGGSGASGTGHR 200

[Close]

BLAST of Moc02g04540 vs. ExPASy TrEMBL
Match: A0A6J1I2E0 (nuclear transcription factor Y subunit B-3-like OS=Cucurbita maxima OX=3661 GN=LOC111469875 PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-86
Identity = 196/211 (92.89%), Postives = 199/211 (94.31%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLK+YLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKSYLQKYREMEGEKSTM 120

Query: 121 -GRQGEKD--GGGGGPGGSGA--GGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHH 180
            G +GEKD  GGGGGPGGSGA  GGGGGGGVN GT GG  GGGFNGVGGMYGGMMMM HH
Sbjct: 121 GGGKGEKDGGGGGGGPGGSGANVGGGGGGGVNPGTGGGVSGGGFNGVGGMYGGMMMMVHH 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           QGGVY GSGGFHHMGIGGGKGG+GGSGTGHR
Sbjct: 181 QGGVY-GSGGFHHMGIGGGKGGNGGSGTGHR 210

[Close]

BLAST of Moc02g04540 vs. ExPASy TrEMBL
Match: A0A6J1G731 (nuclear transcription factor Y subunit B-3-like OS=Cucurbita moschata OX=3662 GN=LOC111451370 PE=3 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 9.5e-86
Identity = 195/211 (92.42%), Postives = 198/211 (93.84%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLK+YLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKSYLQKYREMEGEKSTM 120

Query: 121 -GRQGEKD--GGGGGPGGSGA--GGGGGGGVNSGTAGGAGGGGFNGVGGMYGGMMMMEHH 180
            G +GEKD  GGGGGPGGSGA  GGGGGGGVN GT GG  GGGFNGVGGMYGGMMMM HH
Sbjct: 121 GGGKGEKDGGGGGGGPGGSGANVGGGGGGGVNPGTGGGVSGGGFNGVGGMYGGMMMMGHH 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSGTGHR 207
           QGGVY GSGGFH MGIGGGKGG+GGSGTGHR
Sbjct: 181 QGGVY-GSGGFHQMGIGGGKGGNGGSGTGHR 210

[Close]

BLAST of Moc02g04540 vs. ExPASy TrEMBL
Match: A0A0A0KFU2 (CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G112490 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 4.7e-85
Identity = 190/208 (91.35%), Postives = 193/208 (92.79%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTM 120

Query: 121 GRQGEKDGGGGGPGGSGAGGGGGGGVN-SGTAGGAGGGGFNGVGGMYGGMMMMEHHQGGV 180
           GRQGEKDGGGGGPGGS      GGGVN SG A G  GGG+NGVGGMYGG+MMM HHQGGV
Sbjct: 121 GRQGEKDGGGGGPGGS------GGGVNSSGAAAGGAGGGYNGVGGMYGGVMMMGHHQGGV 180

Query: 181 YGGSGGFHHMGIGGGKGGSGG-SGTGHR 207
           YGG+ GFHHMGIG GKGGSGG SGTGHR
Sbjct: 181 YGGA-GFHHMGIGSGKGGSGGASGTGHR 201

[Close]

BLAST of Moc02g04540 vs. TAIR 10
Match: AT4G14540.1 (nuclear factor Y, subunit B3 )

HSP 1 Score: 211.8 bits (538), Expect = 5.0e-55
Identity = 124/151 (82.12%), Postives = 135/151 (89.40%), Query Frame = 0


Query: 1   MADSDNESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60
           MADSDN+SGGH    N+  S +EQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS
Sbjct: 1   MADSDNDSGGHKDGGNA--STREQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVS 60

Query: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEK-ST 120
           EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFE+YVEPLK YLQKYRE+EGEK +T
Sbjct: 61  EFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQKYREVEGEKTTT 120

Query: 121 MGRQGEKDGGGGGPGGSGAGGGG----GGGV 147
            GRQG+K+GGGGG GG+G+G GG    GGG+
Sbjct: 121 AGRQGDKEGGGGG-GGAGSGSGGAPMYGGGM 148

[Close]

BLAST of Moc02g04540 vs. TAIR 10
Match: AT5G47640.1 (nuclear factor Y, subunit B2 )

HSP 1 Score: 179.9 bits (455), Expect = 2.1e-45
Identity = 124/207 (59.90%), Postives = 141/207 (68.12%), Query Frame = 0


Query: 1   MADSDNESG----GHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQ 60
           M DSD +SG    G+N N  S LS +EQDRFLPIANVSRIMKKALPANAKISKDAKET+Q
Sbjct: 1   MGDSDRDSGGGQNGNNQNGQSSLSPREQDRFLPIANVSRIMKKALPANAKISKDAKETMQ 60

Query: 61  ECVSEFISFITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGE 120
           ECVSEFISF+TGEASDKCQ+EKRKTINGDDLLWAMTTLGFE+YVEPLK YLQ++RE+EGE
Sbjct: 61  ECVSEFISFVTGEASDKCQKEKRKTINGDDLLWAMTTLGFEDYVEPLKVYLQRFREIEGE 120

Query: 121 KSTMGRQGEKDGGGGGPGGSGAGGGGGGGVNSGTAGGAGGGGFNGVGGM-YGGMMMMEHH 180
           ++ +GR                  GG  G +   A G GGG + G GGM Y       H 
Sbjct: 121 RTGLGRPQT---------------GGEVGEHQRDAVGDGGGFYGGGGGMQYHQHHQFLHQ 180

Query: 181 QGGVYGGSGGFHHMGIGGGKGGSGGSG 203
           Q  +YG +GG      G   GG   SG
Sbjct: 181 QNHMYGATGG------GSDSGGGAASG 186

[Close]

BLAST of Moc02g04540 vs. TAIR 10
Match: AT2G37060.1 (nuclear factor Y, subunit B8 )

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-42
Identity = 89/127 (70.08%), Postives = 102/127 (80.31%), Query Frame = 0


Query: 6   NESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISF 65
           +ESGG  S  +  L  +EQDRFLPIAN+SRIMK+ LPAN KI+KDAKE VQECVSEFISF
Sbjct: 15  HESGGDQSPRS--LHVREQDRFLPIANISRIMKRGLPANGKIAKDAKEIVQECVSEFISF 74

Query: 66  ITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTMGRQGE 125
           +T EASDKCQREKRKTINGDDLLWAM TLGFE+Y+EPLK YL +YREMEG+     + G+
Sbjct: 75  VTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREMEGDTKGSAKGGD 134

Query: 126 ----KDG 129
               KDG
Sbjct: 135 PNAKKDG 139

[Close]

BLAST of Moc02g04540 vs. TAIR 10
Match: AT2G37060.2 (nuclear factor Y, subunit B8 )

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-42
Identity = 89/127 (70.08%), Postives = 102/127 (80.31%), Query Frame = 0


Query: 6   NESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISF 65
           +ESGG  S  +  L  +EQDRFLPIAN+SRIMK+ LPAN KI+KDAKE VQECVSEFISF
Sbjct: 15  HESGGDQSPRS--LHVREQDRFLPIANISRIMKRGLPANGKIAKDAKEIVQECVSEFISF 74

Query: 66  ITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTMGRQGE 125
           +T EASDKCQREKRKTINGDDLLWAM TLGFE+Y+EPLK YL +YREMEG+     + G+
Sbjct: 75  VTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREMEGDTKGSAKGGD 134

Query: 126 ----KDG 129
               KDG
Sbjct: 135 PNAKKDG 139

[Close]

BLAST of Moc02g04540 vs. TAIR 10
Match: AT2G37060.3 (nuclear factor Y, subunit B8 )

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-42
Identity = 89/127 (70.08%), Postives = 102/127 (80.31%), Query Frame = 0


Query: 6   NESGGHNSNANSELSAKEQDRFLPIANVSRIMKKALPANAKISKDAKETVQECVSEFISF 65
           +ESGG  S  +  L  +EQDRFLPIAN+SRIMK+ LPAN KI+KDAKE VQECVSEFISF
Sbjct: 15  HESGGDQSPRS--LHVREQDRFLPIANISRIMKRGLPANGKIAKDAKEIVQECVSEFISF 74

Query: 66  ITGEASDKCQREKRKTINGDDLLWAMTTLGFEEYVEPLKTYLQKYREMEGEKSTMGRQGE 125
           +T EASDKCQREKRKTINGDDLLWAM TLGFE+Y+EPLK YL +YREMEG+     + G+
Sbjct: 75  VTSEASDKCQREKRKTINGDDLLWAMATLGFEDYMEPLKVYLMRYREMEGDTKGSAKGGD 134

Query: 126 ----KDG 129
               KDG
Sbjct: 135 PNAKKDG 139

The following BLAST results are available for this feature:

BLAST of Moc02g04540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008464158.1	1.0e-86	92.27	PREDICTED: nuclear transcription factor Y subunit B-3 [Cucumis melo] >XP_0084641...	[more]
XP_022971106.1	2.3e-86	92.89	nuclear transcription factor Y subunit B-3-like [Cucurbita maxima]	[more]
XP_022947534.1	2.0e-85	92.42	nuclear transcription factor Y subunit B-3-like [Cucurbita moschata] >KAG6604918...	[more]
XP_004139999.1	9.8e-85	91.35	nuclear transcription factor Y subunit B-3 [Cucumis sativus] >KGN46611.1 hypothe...	[more]
XP_023532503.1	1.3e-84	92.34	nuclear transcription factor Y subunit B-3-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc02g04540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23310	7.1e-54	82.12	Nuclear transcription factor Y subunit B-3 OS=Arabidopsis thaliana OX=3702 GN=NF...	[more]
Q69J40	1.0e-44	61.82	Nuclear transcription factor Y subunit B-10 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q9FGJ3	3.0e-44	59.90	Nuclear transcription factor Y subunit B-2 OS=Arabidopsis thaliana OX=3702 GN=NF...	[more]
Q75IZ7	1.5e-40	60.00	Nuclear transcription factor Y subunit B-8 OS=Oryza sativa subsp. japonica OX=39...	[more]
Q0J7P4	6.7e-36	58.25	Nuclear transcription factor Y subunit B-11 OS=Oryza sativa subsp. japonica OX=3...	[more]

BLAST of Moc02g04540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CMC5	5.1e-87	92.27	nuclear transcription factor Y subunit B-3 OS=Cucumis melo OX=3656 GN=LOC1035021...	[more]
A0A5A7V4W8	5.1e-87	92.27	Nuclear transcription factor Y subunit B-3 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A6J1I2E0	1.1e-86	92.89	nuclear transcription factor Y subunit B-3-like OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1G731	9.5e-86	92.42	nuclear transcription factor Y subunit B-3-like OS=Cucurbita moschata OX=3662 GN...	[more]
A0A0A0KFU2	4.7e-85	91.35	CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G1124...	[more]

BLAST of Moc02g04540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14540.1	5.0e-55	82.12	nuclear factor Y, subunit B3	[more]
AT5G47640.1	2.1e-45	59.90	nuclear factor Y, subunit B2	[more]
AT2G37060.1	1.3e-42	70.08	nuclear factor Y, subunit B8	[more]
AT2G37060.2	1.3e-42	70.08	nuclear factor Y, subunit B8	[more]
AT2G37060.3	1.3e-42	70.08	nuclear factor Y, subunit B8	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00615	CCAATSUBUNTA	coord: 93..111 score: 44.86 coord: 55..73 score: 50.24 coord: 74..92 score: 38.52
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 114..144
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..21
None	No IPR available	PANTHER	PTHR11064:SF128	NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-3	coord: 1..181
IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain	PFAM	PF00808	CBFD_NFYB_HMF	coord: 27..91 e-value: 2.0E-27 score: 95.2
IPR009072	Histone-fold	GENE3D	1.10.20.10	Histone, subunit A	coord: 15..131 e-value: 1.2E-47 score: 163.3
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 24..126
IPR027113	Transcription factor NFYB/HAP3	PANTHER	PTHR11064	CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED	coord: 1..181
IPR003956	Transcription factor, NFYB/HAP3, conserved site	PROSITE	PS00685	NFYB_HAP3	coord: 58..74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g04540.1	Moc02g04540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0045944	positive regulation of transcription by RNA polymerase II
cellular_component	GO:0016602	CCAAT-binding factor complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0001228	DNA-binding transcription activator activity, RNA polymerase II-specific
molecular_function	GO:0016853	isomerase activity
molecular_function	GO:0046982	protein heterodimerization activity
molecular_function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function	GO:0043565	sequence-specific DNA binding