Moc03g33490 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g33490
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPectate lyase
Locationchr3: 23724761 .. 23729359 (-)
RNA-Seq ExpressionMoc03g33490
SyntenyMoc03g33490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACGCCATCGACAGATGCTGGAGATCCAACCCATATTGGAGGAGAAACAGGCAACAGCTAGGTGTTTGTTCGGTGGGTTTTGTGGGGAAAATGACCAACAACATCGGAAGAAACCTCATACGCTACCAAGTCACTGACCCAAGTGACGATCCCATAAACCCTCGCCCGGGTACCCTTAGATATGGCGCCTCTGTCATCAGAGGCAAGGTGTGGATCACATTTCGGAGGGACATGCGTATCACGCTTCACAAACCGCTCATGATTAGTAGCTTCACTGCCATCGATGGCCGAGGTGCGACCGTCCATATTGCAGGGAATGCGTGCCTGATGGTGCTTAGGGCCAGCAATATTATAATCCACGGGCTTATTATCCACCACTGCAGGGCCCAGGCCGCAGCCCAAGTGATGGGCCCAAATTCCAAGATCATTTCCTTAGGACACGTGGATGGAGATGCCATTAGATTAGTTACGGCCTCGAAGGTTTGGATCGACCACAACACGCTGTACCGATGCGAGGACGGATTGCTGGATGTGACCAGAGGGTCCACCGACATTACGATTTCGAACAATTGGTTTAGAGATCAGGACAAGGTTATACTTCTGGGACATGACGATGGTTACGTTAGAGATCGGAGGATGAAAGTGACGGTGGTGTACAACCATTTTGGACCCAACTGCAACCAGAGGATGCCAAGGTGATTAAATTAACAAACATTATATTATTGGTATTGTATTTGTTGTGGTAACATGCTTCGAGATTAAAATTAAATTGTTAATTAGGATTCGATATGGGTATGCACACGTGGCAAACAATATGTACCAAGGGTGGACGCAATACGCGATAGGAGGGAGCATGAATCCAAGCGTGAAGAGTGAAGCCAACCTTTTCATCGCATCAAAATCGAAAGAGGCAAGATATGATTGGGTTTGATAATTATATAATATTTATTTTATTTTATGAAAATTAATAAAAGAAAATATTGAATTGGCGGTTGCAGGTGGTATGGAGTACAGGAAACGTGGAGGAGACAAAGTGGAATTTTCATTCAGTGAGAGACGTTTTTGAGAATGGGGCTTCGTTTGCACAAATTGGCGTTGGCCGAGGAGTGGTAACGCCAAACTACAACGCGCAGCAACGGTTTCAAGTGGCAGACGCCAAGTGGGTGAGGTCGTTGACCACATCCTCAGGGGCTTTGCGTTGCGCACGAAACTCAAGGTGTTAAATCCTTTTTATCTCGACCAATAACACCTCGCCCATCATCATCATCATCAATATAATGGAACCTCTTCACGGTTTTATTTTATAAATAAATGGATTCTATCTATATTTTGATAAATCCACCTACAATAAAACTATATATATATATGAGCAGATGAGAGACAATTTTTTTTTCCTTTTAATATTGCCATTTAATCGATCTCTAGACTCAAGTAATTAATGAGGATATGGGATGGTGTAATCCACTCTTTCTCGTGCTTTGTATCATCTTAAATTTAGTTCAATGCAATATATTAATGAGCATCTCCAAACTTTTTTAATAGTTTCTTTGTACTACATTTTTTTCCCCCACAAACTTCTTCTCCATACATATATACATATACACCAAATAAGAAAAAAAAAATACAAGTATAGTATGACATTATTTGTTTTAGACATAAATCTCACCGCCGTTTTAGTTTTGGTTTTACCTAAAAAAATCTCAATAATGGTCTCGTCTATATACTATAAATTACTTGATCTATATGTTTTAGTCATGGGTGTGATGACTTTGTTTAAATAGTTTGCAATCCCAATCTATCCGACCTTTATTTTGTGAGTTATATACATAAGAATATGTGTGACAGTACTAGTCCATGAGTAATGGATGATATAAAATCATAAACATATTGCGATGTTGATCTGTGTTGTAGAATATATGGGCACATTGAAAGTTGAAGAAATGAGAAATAAAGGGGAAGAATTGACGAACACACTGAACGAAAGAGGCTTTAGTTCAATTATGATAGAAGGAATAATATTGAAAAACTAACTTAATTAACTTAACTGCTGCAGCAGAGCAGGGTGTAGGGAGTAGGTGCAGAATGTGGCAGCACGCGAGCGATGCCGACATTCCAAATATGGTCAATTAAAAGTACATATATATATATATATATATATTAAAAACACAGACACGCAGAGAGAGAGAGAGAGAGAGAGAGAACTTGGAGGATACGTATAGTGTAGTGTGTGTGAAAGAGAGAGATGAGAGATGAGATGGGAGGCGGTGGTGGAGTTACAATAAGTATAACTAAAGGGCTTCCGGGAAACGTTTGTCCAAGAATCTCCAAAAGAGAAAAGAAAAAAAGAAAAAGAAAACGATCCCAATCCCAATCCCAATCCCAGTCCCCCGGCCGCCTGCTTTTAGTTATAAGTTTCAACCATGTATGTGTGTATATATAGGGCGGTGTGGCGATACATGTGGAGAAGAAAGGAAAGAAAAGTATGGCGCCAGTGGGTCTGCCTCCAGGGTTCAGGTTTCACCCGACGGACGAGGAGCTGGTGAACTACTACCTGAAGAGGAAAATCAGCGGGCAAGAAATAGAGCTGGATATAATCCCGGAGGTGGACCTGTACAAATGCGAGCCTTGGGAGCTGTCGGACAAGTCGTTTCTGCCCAGTAGAGACCCGGAGTGGTACTTCTTCGGCCCACGAGACAGAAAGTATCCCAACGGCTTCAGAACCAACAGGGCCACCAGAGCCGGCTACTGGAAATCAACCGGAAAGGACCGCCGGGTCACCTCACAGAACCGCCCCATTGGGATGAAGAAGACCCTCGTTTACTACAGGGGCCGAGCCCCCCAGGGCATCAGGACCGACTGGGTCATGCATGAATATCGCCTCCACGACAACGACTACTGCGACAGCGACACCTCCGCCATCCAGGTATTTCCTAATTTAATTTGCAATTTTACTTACTTTACTTATATACCTTTAATTAATTTGGAATGATTACCTATTACCGACTTCTTTTCCTTTTATATATTGTTGGGTTCCGTTCAAAAGATTTTTTTGCATTTGTTGTTGTTGGTTGGCTGGCTAAGGGTTCTCAAGCTCTCTAAAGTTCGCCACATTCTTCCAAATTAAAGGTCTAAAACTAGTGCTCAAATCATATCAATCAAAGGACTCCACCGAAAGCTGTTTTCCTCAGACTCTCATCCACCATTTATTTATCAAACTTATCAACTTTGACTTCTACTGTCTCCTTCGCTGACACATGCACGCATGCTTCTCTCTTTTTTCTTAAAACAAAAACATGTTTCTTTACTCTTTTTTTTTCCCTTGCTTTCAAGAATCTTTGGTTTTAAGCTCATTTATAATTTAGTACCTAAACTTTTGAAATTATTTTAGATGCTTAAAGTCTAAAGACCTACGAGATTATACTATTCTTAAAAATAGTTTTTCATGAACTAGAGCAAGTTGCTGACACGCCTCCTGTGGTCGGACTTATAAAAAGATAAAATTGAACTTAATTGTAAAATTTTGCCTCTCTACAAGAGGGACGGGGGTGTAATAATATTAATAGAATTGTAGAGGGCACAGCAAAACAGCATGACAAAAATGGACATAAATGGGTGACATATTCTTTACTTGTACCAGGATCTGTGAAGCGATAAAGTTGGGTTTGGGTTTGGGTTTTGTTTTGATATGGCAGATGAGACAAAAGTGCAATCTAATTCCCCCTATGACTTAAAACACACCAGCTCTTATTTTGTTTGGTTGTAAAAGAAGATGTCTCCCACTTTTCACTTTATTCCAATCACCAAAATCACCCAATGGATCTCTCTTTGTCCACGAGTTTTACATACGATACAACTAATTAACTTACCTTTCTTTCTCTCTCCCTCTCCCTTCATTTAAATATGACCTAATAACATCCTCTTTTTCTTTGTTTTCTCTCAACTTTATATATATAAATAACATTTTAATTAGACGATTCTCATTCTATTTCTGCTTTTACTTTCAATACTATTACTATGGAGTCTTTATGCATGGATTTAGAACATAATCGAAACATCTAAAATTTGTATTAAAGGCGATGCAAATTCAATTTTGTTAAGATATCTTAATTAAAGTAAAAGAGTGTAGATTAAAATAAGTGTAAAAGGTGTCGAAGAATGGCTGAAATTTTTAAATATAAAGTAGTTAGAAGAGAAGAGTAACGGAGAGATAAAGTCAACTCGGAAAATTGGAAGTGTCGTTTTTTTGTTTTATTTTTATATTGGGGGTTGCAGGATTCTTACGCCTTGTGTAGAGTATTCAAGAAAAATGGAATATGTTCGGAAGTGGAAGAAGCAGGAGGACAGAGCAGCAGCAGCGGCTTGTCGTTCATGGACAACTCAACGTCACAAACTTTGATGAATCATGACTACGAGACTCTGTCACCGGATGTTCTGATGGCGGCTTCGTCTTCTTGTGTGGAGGAGGAAGAGAAGGACGATTCCTGGATGCAGTTCATTACAGAAGATGCATGGTGCGCTTCCAATTCATCACCCATCGCCGGCGCCGGCGCCGGCCTCGATGACCTCTCTCATCTGACTTTCACCAATTTATCATAA

mRNA sequence

ATGAACGCCATCGACAGATGCTGGAGATCCAACCCATATTGGAGGAGAAACAGGCAACAGCTAGGTGTTTGTTCGGTGGGTTTTGTGGGGAAAATGACCAACAACATCGGAAGAAACCTCATACGCTACCAAGTCACTGACCCAAGTGACGATCCCATAAACCCTCGCCCGGGTACCCTTAGATATGGCGCCTCTGTCATCAGAGGCAAGGTGTGGATCACATTTCGGAGGGACATGCGTATCACGCTTCACAAACCGCTCATGATTAGTAGCTTCACTGCCATCGATGGCCGAGGTGCGACCGTCCATATTGCAGGGAATGCGTGCCTGATGGTGCTTAGGGCCAGCAATATTATAATCCACGGGCTTATTATCCACCACTGCAGGGCCCAGGCCGCAGCCCAAGTGATGGGCCCAAATTCCAAGATCATTTCCTTAGGACACGTGGATGGAGATGCCATTAGATTAGTTACGGCCTCGAAGGTTTGGATCGACCACAACACGCTGTACCGATGCGAGGACGGATTGCTGGATGTGACCAGAGGGTCCACCGACATTACGATTTCGAACAATTGGTTTAGAGATCAGGACAAGGTTATACTTCTGGGACATGACGATGGTTACGTTAGAGATCGGAGGATGAAAGTGACGGTGGTGTACAACCATTTTGGACCCAACTGCAACCAGAGGATGCCAAGGATTCGATATGGGTATGCACACGTGGCAAACAATATGTACCAAGGGTGGACGCAATACGCGATAGGAGGGAGCATGAATCCAAGCGTGAAGAGTGAAGCCAACCTTTTCATCGCATCAAAATCGAAAGAGGCAAGATATGATTGGGTGGTATGGAGTACAGGAAACGTGGAGGAGACAAAGTGGAATTTTCATTCAGTGAGAGACGTTTTTGAGAATGGGGCTTCGTTTGCACAAATTGGCGTTGGCCGAGGAGTGGTAACGCCAAACTACAACGCGCAGCAACGGTTTCAAGTGGCAGACGCCAAGTGGGGCGGTGTGGCGATACATGTGGAGAAGAAAGGAAAGAAAAGTATGGCGCCAGTGGGTCTGCCTCCAGGGTTCAGGTTTCACCCGACGGACGAGGAGCTGGTGAACTACTACCTGAAGAGGAAAATCAGCGGGCAAGAAATAGAGCTGGATATAATCCCGGAGGTGGACCTGTACAAATGCGAGCCTTGGGAGCTGTCGGACAAGTCGTTTCTGCCCAGTAGAGACCCGGAGTGGTACTTCTTCGGCCCACGAGACAGAAAGTATCCCAACGGCTTCAGAACCAACAGGGCCACCAGAGCCGGCTACTGGAAATCAACCGGAAAGGACCGCCGGGTCACCTCACAGAACCGCCCCATTGGGATGAAGAAGACCCTCGTTTACTACAGGGGCCGAGCCCCCCAGGGCATCAGGACCGACTGGGTCATGCATGAATATCGCCTCCACGACAACGACTACTGCGACAGCGACACCTCCGCCATCCAGGATTCTTACGCCTTGTGTAGAGTATTCAAGAAAAATGGAATATGTTCGGAAGTGGAAGAAGCAGGAGGACAGAGCAGCAGCAGCGGCTTGTCGTTCATGGACAACTCAACGTCACAAACTTTGATGAATCATGACTACGAGACTCTGTCACCGGATGTTCTGATGGCGGCTTCGTCTTCTTGTGTGGAGGAGGAAGAGAAGGACGATTCCTGGATGCAGTTCATTACAGAAGATGCATGGTGCGCTTCCAATTCATCACCCATCGCCGGCGCCGGCGCCGGCCTCGATGACCTCTCTCATCTGACTTTCACCAATTTATCATAA

Coding sequence (CDS)

ATGAACGCCATCGACAGATGCTGGAGATCCAACCCATATTGGAGGAGAAACAGGCAACAGCTAGGTGTTTGTTCGGTGGGTTTTGTGGGGAAAATGACCAACAACATCGGAAGAAACCTCATACGCTACCAAGTCACTGACCCAAGTGACGATCCCATAAACCCTCGCCCGGGTACCCTTAGATATGGCGCCTCTGTCATCAGAGGCAAGGTGTGGATCACATTTCGGAGGGACATGCGTATCACGCTTCACAAACCGCTCATGATTAGTAGCTTCACTGCCATCGATGGCCGAGGTGCGACCGTCCATATTGCAGGGAATGCGTGCCTGATGGTGCTTAGGGCCAGCAATATTATAATCCACGGGCTTATTATCCACCACTGCAGGGCCCAGGCCGCAGCCCAAGTGATGGGCCCAAATTCCAAGATCATTTCCTTAGGACACGTGGATGGAGATGCCATTAGATTAGTTACGGCCTCGAAGGTTTGGATCGACCACAACACGCTGTACCGATGCGAGGACGGATTGCTGGATGTGACCAGAGGGTCCACCGACATTACGATTTCGAACAATTGGTTTAGAGATCAGGACAAGGTTATACTTCTGGGACATGACGATGGTTACGTTAGAGATCGGAGGATGAAAGTGACGGTGGTGTACAACCATTTTGGACCCAACTGCAACCAGAGGATGCCAAGGATTCGATATGGGTATGCACACGTGGCAAACAATATGTACCAAGGGTGGACGCAATACGCGATAGGAGGGAGCATGAATCCAAGCGTGAAGAGTGAAGCCAACCTTTTCATCGCATCAAAATCGAAAGAGGCAAGATATGATTGGGTGGTATGGAGTACAGGAAACGTGGAGGAGACAAAGTGGAATTTTCATTCAGTGAGAGACGTTTTTGAGAATGGGGCTTCGTTTGCACAAATTGGCGTTGGCCGAGGAGTGGTAACGCCAAACTACAACGCGCAGCAACGGTTTCAAGTGGCAGACGCCAAGTGGGGCGGTGTGGCGATACATGTGGAGAAGAAAGGAAAGAAAAGTATGGCGCCAGTGGGTCTGCCTCCAGGGTTCAGGTTTCACCCGACGGACGAGGAGCTGGTGAACTACTACCTGAAGAGGAAAATCAGCGGGCAAGAAATAGAGCTGGATATAATCCCGGAGGTGGACCTGTACAAATGCGAGCCTTGGGAGCTGTCGGACAAGTCGTTTCTGCCCAGTAGAGACCCGGAGTGGTACTTCTTCGGCCCACGAGACAGAAAGTATCCCAACGGCTTCAGAACCAACAGGGCCACCAGAGCCGGCTACTGGAAATCAACCGGAAAGGACCGCCGGGTCACCTCACAGAACCGCCCCATTGGGATGAAGAAGACCCTCGTTTACTACAGGGGCCGAGCCCCCCAGGGCATCAGGACCGACTGGGTCATGCATGAATATCGCCTCCACGACAACGACTACTGCGACAGCGACACCTCCGCCATCCAGGATTCTTACGCCTTGTGTAGAGTATTCAAGAAAAATGGAATATGTTCGGAAGTGGAAGAAGCAGGAGGACAGAGCAGCAGCAGCGGCTTGTCGTTCATGGACAACTCAACGTCACAAACTTTGATGAATCATGACTACGAGACTCTGTCACCGGATGTTCTGATGGCGGCTTCGTCTTCTTGTGTGGAGGAGGAAGAGAAGGACGATTCCTGGATGCAGTTCATTACAGAAGATGCATGGTGCGCTTCCAATTCATCACCCATCGCCGGCGCCGGCGCCGGCCTCGATGACCTCTCTCATCTGACTTTCACCAATTTATCATAA

Protein sequence

MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWGGVAIHVEKKGKKSMAPVGLPPGFRFHPTDEELVNYYLKRKISGQEIELDIIPEVDLYKCEPWELSDKSFLPSRDPEWYFFGPRDRKYPNGFRTNRATRAGYWKSTGKDRRVTSQNRPIGMKKTLVYYRGRAPQGIRTDWVMHEYRLHDNDYCDSDTSAIQDSYALCRVFKKNGICSEVEEAGGQSSSSGLSFMDNSTSQTLMNHDYETLSPDVLMAASSSCVEEEEKDDSWMQFITEDAWCASNSSPIAGAGAGLDDLSHLTFTNLS
Homology
BLAST of Moc03g33490 vs. NCBI nr
Match: KAG6584442.1 (putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1004.6 bits (2596), Expect = 3.6e-289
Identity = 505/642 (78.66%), Postives = 536/642 (83.49%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNN+GRNLIRYQVTDPSDDPINPRPGTL
Sbjct: 30  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNVGRNLIRYQVTDPSDDPINPRPGTL 89

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RYGA++I+ KVWITF++DM I LHKPL+ISS+TAIDGRGA+VHI+GNACL+V +A+NIII
Sbjct: 90  RYGATMIKSKVWITFQKDMHILLHKPLLISSYTAIDGRGASVHISGNACLVVSKATNIII 149

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HGLIIHHC+AQ A QVMGPNSK++SLGHVDGDAIRLV+ASKVWIDHNTLYRCEDGLLDVT
Sbjct: 150 HGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIRLVSASKVWIDHNTLYRCEDGLLDVT 209

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
           RGSTDITISNNWFRDQDKVILLGH+DGYVRD+ M+VTVVYNHFGPNCNQRMPRIR+GYAH
Sbjct: 210 RGSTDITISNNWFRDQDKVILLGHEDGYVRDKNMRVTVVYNHFGPNCNQRMPRIRHGYAH 269

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 300
           VANN+YQGWTQYAIGGSMNPS                    +VWSTG VE  KW FHSVR
Sbjct: 270 VANNLYQGWTQYAIGGSMNPS--------------------IVWSTGKVEAAKWKFHSVR 329

Query: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKWG----------------------- 360
           DVFENGASF QIGVGRG V PNY+AQQRF VADAKW                        
Sbjct: 330 DVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKWARLTEAVFGLLCRPGRHDAFSSSI 389

Query: 361 ---GVAIH---------VEKKGKKSMAPVGLPPGFRFHPTDEELVNYYLKRKISGQEIEL 420
               V +          V K+  K MAPVGLPPGFRFHPTDEELVNYYLKRKISGQ IEL
Sbjct: 390 SYFAVVVSLSESWARRVVGKELGKEMAPVGLPPGFRFHPTDEELVNYYLKRKISGQVIEL 449

Query: 421 DIIPEVDLYKCEPWELSDKSFLPSRDPEWYFFGPRDRKYPNGFRTNRATRAGYWKSTGKD 480
           DIIPEVDLYKCEPWELS+KSFLPSRDPEWYFFGPRDRKYPNGFRTNRATRAGYWKSTGKD
Sbjct: 450 DIIPEVDLYKCEPWELSEKSFLPSRDPEWYFFGPRDRKYPNGFRTNRATRAGYWKSTGKD 509

Query: 481 RRVTSQNRPIGMKKTLVYYRGRAPQGIRTDWVMHEYRLHDNDYCDSDTSAIQDSYALCRV 540
           RRVTS  R IGMKKTLVYYRGRAPQGIRTDWVMHEYRL D D C+ D+S IQDSYALCRV
Sbjct: 510 RRVTSHQRAIGMKKTLVYYRGRAPQGIRTDWVMHEYRLDDKD-CE-DSSGIQDSYALCRV 569

Query: 541 FKKNGICSEVEE-AGGQ--SSSSGLSFMD--NSTSQTLMNHDYETLSPDVLM-AASSSCV 600
            KKNGICSEVEE   GQ  SSSS LSF+D  NSTSQTL+N DYETLSPDVLM   SSSCV
Sbjct: 570 LKKNGICSEVEEQISGQSSSSSSSLSFIDNSNSTSQTLVN-DYETLSPDVLMPPPSSSCV 629

Query: 601 EEEEKDDSWMQFITEDAWCASNSSPIAGAGAGLDDLSHLTFT 602
           EEEEKDDSWMQFITEDAWCASNSSPIA     L DLSHLTFT
Sbjct: 630 EEEEKDDSWMQFITEDAWCASNSSPIA-----LHDLSHLTFT 643

BLAST of Moc03g33490 vs. NCBI nr
Match: XP_022137718.1 (putative pectate lyase 2 isoform X1 [Momordica charantia])

HSP 1 Score: 692.2 bits (1785), Expect = 4.0e-195
Identity = 332/339 (97.94%), Postives = 333/339 (98.23%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL
Sbjct: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII
Sbjct: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
           RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDW---VVWSTGNVEETKWNFH 300
           VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE   +W   VVWSTGNVEETKWNFH
Sbjct: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEKILNWRLQVVWSTGNVEETKWNFH 300

Query: 301 SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 337
           SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW
Sbjct: 301 SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 339

BLAST of Moc03g33490 vs. NCBI nr
Match: XP_022137719.1 (putative pectate lyase 2 isoform X2 [Momordica charantia])

HSP 1 Score: 688.7 bits (1776), Expect = 4.4e-194
Identity = 331/336 (98.51%), Postives = 331/336 (98.51%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL
Sbjct: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII
Sbjct: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
           RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 300
           VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE     VVWSTGNVEETKWNFHSVR
Sbjct: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE-----VVWSTGNVEETKWNFHSVR 300

Query: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 337
           DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW
Sbjct: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 331

BLAST of Moc03g33490 vs. NCBI nr
Match: XP_008439120.1 (PREDICTED: putative pectate lyase 2 [Cucumis melo])

HSP 1 Score: 632.9 bits (1631), Expect = 2.9e-177
Identity = 297/336 (88.39%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MNAIDRCWRSNPYWRRNRQQL  CSVGF GKMTNN+GRNLI YQVTDPSDDPINPRPGTL
Sbjct: 33  MNAIDRCWRSNPYWRRNRQQLATCSVGFAGKMTNNMGRNLIHYQVTDPSDDPINPRPGTL 92

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RYGA++I+ KVWITF++DM I L KPL+ISSFTAIDGRGATVHI GNACLMVLRA+NIII
Sbjct: 93  RYGATMIKHKVWITFQKDMHIVLQKPLLISSFTAIDGRGATVHITGNACLMVLRATNIII 152

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HGLIIHHCR+QAA +VMGPNSK+ISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 153 HGLIIHHCRSQAAGEVMGPNSKVISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 212

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
           RGSTDITISNNWFRDQDKVILLGHDDGYVRDR MKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 213 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRNMKVTVVYNHFGPNCNQRMPRIRYGYAH 272

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 300
           VANN+YQGWTQYAIGGSMNPSVKSEANLFIASKSK+     V+WSTGNVEE KW FHSVR
Sbjct: 273 VANNLYQGWTQYAIGGSMNPSVKSEANLFIASKSKQ-----VIWSTGNVEEAKWKFHSVR 332

Query: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 337
           DVFENGA F +IGVGRG V PNYN QQRF VADAKW
Sbjct: 333 DVFENGALFTEIGVGRGRVKPNYNPQQRFPVADAKW 363

BLAST of Moc03g33490 vs. NCBI nr
Match: XP_038896018.1 (putative pectate lyase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 631.3 bits (1627), Expect = 8.4e-177
Identity = 295/336 (87.80%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MNAIDRCWRSNPYWRRNRQQL +CS+GF GKMTNNIGRNLI+YQVTDPSDDP NPRPGTL
Sbjct: 1   MNAIDRCWRSNPYWRRNRQQLAICSIGFAGKMTNNIGRNLIQYQVTDPSDDPTNPRPGTL 60

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RYGA++I+ KVWITFR+DM I L KPL+ISSFTAIDGRGATVHI GNACLMV +A+NIII
Sbjct: 61  RYGATMIKSKVWITFRKDMHIVLKKPLLISSFTAIDGRGATVHITGNACLMVFKATNIII 120

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HGLIIHHCRAQAA QVMGPNSKII+LGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 121 HGLIIHHCRAQAAGQVMGPNSKIITLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
           RGSTDITISNNWFRDQDKVILLGHDDGYVRDR M+VTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRNMRVTVVYNHFGPNCNQRMPRIRYGYAH 240

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 300
           VANN+YQGWTQYAIGGSMNPSVKSEANLFIASKSK+     ++WSTG VE+ KW FHSVR
Sbjct: 241 VANNLYQGWTQYAIGGSMNPSVKSEANLFIASKSKQ-----IIWSTGKVEQAKWKFHSVR 300

Query: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 337
           DVFENGA FAQIG GRG V PNYNAQQRF VADAKW
Sbjct: 301 DVFENGALFAQIGSGRGNVKPNYNAQQRFPVADAKW 331

BLAST of Moc03g33490 vs. ExPASy Swiss-Prot
Match: O65388 (Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2)

HSP 1 Score: 314.3 bits (804), Expect = 3.0e-84
Identity = 163/341 (47.80%), Postives = 214/341 (62.76%), Query Frame = 0

Query: 2   NAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTLR 61
           N ID CWR NPYW  NR+ L  C+VGF GK     G+    Y VT+PSDDP NPRPGTLR
Sbjct: 37  NPIDSCWRRNPYWASNRRALADCAVGF-GKSAVG-GKYGSIYVVTNPSDDPENPRPGTLR 96

Query: 62  YGASVIRGK-VWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 121
           Y  +VI+ K +WITF RDM I L   L+++S+  IDGRGA V IA   C+ +   S++II
Sbjct: 97  Y--AVIQSKPLWITFARDMVIVLRNELIMNSYKTIDGRGAKVEIAYGPCITIQHVSHVII 156

Query: 122 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 181
           HG+ IH C+   + +V    + + S    DGDAI +  +S +WIDH    RC+DGL+DV 
Sbjct: 157 HGISIHDCKPGKSGRVRSSPTHVGSRKGSDGDAIAIFDSSHIWIDHCFFSRCQDGLIDVL 216

Query: 182 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 241
             ST +TISNN+F   DKV+LLGH+D  V D+ M+VT+ +NHFGP   +RMPR+R GYAH
Sbjct: 217 HASTAVTISNNYFTQHDKVMLLGHNDNNVEDKIMRVTIAFNHFGPGLIERMPRVRRGYAH 276

Query: 242 VANNMYQGWTQYAIGGSMNPSVKSEANLFIAS---------KSKEARYDWVVWSTGNVEE 301
           VANN Y+ W  YAIGGS +P++ SE N F+AS         K  ++ YDW          
Sbjct: 277 VANNRYEKWQMYAIGGSADPTIFSEGNYFVASDDPSKKQVTKRIDSGYDW---------- 336

Query: 302 TKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVA 333
            +W + + +DVF+NGA F  +  G G VTP Y   +RF V+
Sbjct: 337 KRWKWRTSKDVFKNGAYF--VPSGYGTVTPLYGRAERFPVS 361

BLAST of Moc03g33490 vs. ExPASy Swiss-Prot
Match: Q9C8G4 (Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.2e-80
Identity = 159/332 (47.89%), Postives = 204/332 (61.45%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           +N ID CWR NP W  NRQ L  C+VG+ GK     G+N   Y VT+PSD+P  P PGTL
Sbjct: 35  LNPIDACWRRNPKWATNRQALAHCAVGY-GKAAIG-GKNGPIYVVTNPSDNPTRPSPGTL 94

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RY  S  +  +WITF RDM I L   LMI+S+  IDGRGA V IA   CL + +  ++II
Sbjct: 95  RYAVSQPK-PLWITFARDMVIVLKSQLMINSYKTIDGRGAKVEIANGPCLRIRQVKHVII 154

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HG+ IH C+A        PN        +DGD IR+  ++ VWIDH  L RC DGL+DV 
Sbjct: 155 HGISIHDCKAD-------PNG-------MDGDGIRVFQSTHVWIDHCFLSRCHDGLIDVI 214

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
             ST +TISNN+F   DKV+LLGHDD Y+ D+ M+VT+ +N FGP   +RMPR+R GYAH
Sbjct: 215 VSSTAVTISNNYFTQHDKVMLLGHDDSYMGDKDMRVTIAFNTFGPGLIERMPRVRRGYAH 274

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 300
           VANN Y+ W  YAIGGS NP + SE N F+A + + ++        G  +  +W + + R
Sbjct: 275 VANNRYEKWQMYAIGGSANPIIFSEGNYFVAPEKRSSKQVTKRMMAG-PDSKRWKWGTSR 334

Query: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVA 333
           DVF NGA F   GV   +V P Y   + F+VA
Sbjct: 335 DVFMNGAFFGPPGV---IVRPLYKGGEGFRVA 345

BLAST of Moc03g33490 vs. ExPASy Swiss-Prot
Match: Q9LRM5 (Probable pectate lyase 9 OS=Arabidopsis thaliana OX=3702 GN=At3g24230 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 2.2e-79
Identity = 151/315 (47.94%), Postives = 192/315 (60.95%), Query Frame = 0

Query: 2   NAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDP-INPRPGTL 61
           N ID CWR +  W +NR+ L  C +GF  K     GRN   Y VTD SDD  +NP+PGTL
Sbjct: 104 NLIDDCWRCDRNWNKNRKHLADCGMGFGSKAFG--GRNGSYYVVTDHSDDDVVNPKPGTL 163

Query: 62  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 121
           R+    +   +WI F+RDM I L + L+++SF  ID RGA VHIA  AC+ +   +N+I+
Sbjct: 164 RHAVIQVE-PLWIIFKRDMVIKLKQELIMNSFKTIDARGANVHIANGACITIQNITNVIV 223

Query: 122 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 181
           HGL IH C+      V    S+    G  DGDAI +  +S +WIDHN+L  C DGL+DV 
Sbjct: 224 HGLHIHDCKRTGNVTVRSSPSQAGFRGTADGDAINIFGSSHIWIDHNSLSNCTDGLVDVV 283

Query: 182 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 241
            GST ITISNN F   D+V+LLGH+D Y RD+ M+VTV YNHFG    QRMPR R+GY H
Sbjct: 284 NGSTAITISNNHFTHHDEVMLLGHNDSYTRDKMMQVTVAYNHFGEGLIQRMPRCRHGYFH 343

Query: 242 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 301
           V NN Y  W  YAIGGS NP++ S+ N F A K+  A+       T   E  +WN+ S +
Sbjct: 344 VVNNDYTHWKMYAIGGSANPTINSQGNRFAAPKNHSAKEVTKRLDTKGNEWMEWNWRSEK 403

Query: 302 DVFENGASFAQIGVG 316
           D+  NGA F   G G
Sbjct: 404 DLLVNGAFFTPSGEG 415

BLAST of Moc03g33490 vs. ExPASy Swiss-Prot
Match: Q9SVQ6 (Putative pectate lyase 14 OS=Arabidopsis thaliana OX=3702 GN=At4g13210 PE=3 SV=2)

HSP 1 Score: 295.0 bits (754), Expect = 1.9e-78
Identity = 151/315 (47.94%), Postives = 190/315 (60.32%), Query Frame = 0

Query: 2   NAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSD-DPINPRPGTL 61
           N ID CWR +  W R R++L  C++GF        GR+   Y VTDPSD DP+ P+PGTL
Sbjct: 90  NPIDDCWRCDKKWHRRRKRLADCAIGFGRNAVG--GRDGRYYIVTDPSDHDPVTPKPGTL 149

Query: 62  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 121
           RY A +    +WI F+RDM ITL + L+++SF  IDGRG  VHIAG ACL V   +NIII
Sbjct: 150 RY-AVIQDEPLWIVFKRDMVITLSQELIMNSFKTIDGRGVNVHIAGGACLTVQYVTNIII 209

Query: 122 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 181
           HG+ IH C+    A V    S        DGD I +  +S +WIDHN+L  C DGL+D  
Sbjct: 210 HGINIHDCKRTGNAMVRSSESHYGWRTMADGDGISIFGSSHIWIDHNSLSSCADGLIDAI 269

Query: 182 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 241
            GST ITISNN+    ++ ILLGH D Y RD+ M+VT+ YNHFG    QRMPR R+GY H
Sbjct: 270 MGSTAITISNNYLTHHNEAILLGHTDSYTRDKMMQVTIAYNHFGEGLIQRMPRCRHGYFH 329

Query: 242 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 301
           V NN Y  W  YAIGGS NP++ S+ N F+A  ++ A+        G  E   WN+ S  
Sbjct: 330 VVNNDYTHWEMYAIGGSANPTINSQGNRFLAPGNRFAKEVTKRVGAGKGEWNNWNWRSQG 389

Query: 302 DVFENGASFAQIGVG 316
           D+  NGA F   G G
Sbjct: 390 DLMLNGAYFTSSGAG 401

BLAST of Moc03g33490 vs. ExPASy Swiss-Prot
Match: O65456 (Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.9e-78
Identity = 158/336 (47.02%), Postives = 204/336 (60.71%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MN +D CWR    W  NR+ L  C VGF          NL  Y VT+P D+  NP+PG+L
Sbjct: 46  MNPVDSCWRLKSDWAANRKDLADCVVGFGSSTLGGKKGNL--YVVTNPYDNAQNPQPGSL 105

Query: 61  RYGASVIRGK-VWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNII 120
           RYG  VI+ K +WITF +DM ITL   LM++S+  IDGRGA V IA   C+ +   +N+I
Sbjct: 106 RYG--VIQAKPLWITFAKDMVITLENELMVNSYKTIDGRGAKVEIAYGPCITIQDVTNVI 165

Query: 121 IHGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDV 180
           +HG+ IH C+      V    + +      DGDAI +  +S +WIDH  L  C DGL+DV
Sbjct: 166 VHGISIHDCKPGKYGMVRSSPTHVGHRKGSDGDAIAIFGSSNIWIDHCYLASCTDGLIDV 225

Query: 181 TRGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYA 240
              ST ITISNN+F   DKV+LLGH+D +V+D +MKVTV +NHFGP   +RMPR+R GYA
Sbjct: 226 IHASTGITISNNYFTQHDKVMLLGHNDDFVQDVKMKVTVAFNHFGPGLVERMPRVRRGYA 285

Query: 241 HVANNMYQGWTQYAIGGSMNPSVKSEANLFIA---SKSKEARYDWVVWSTGNVEETKWNF 300
           HVANN Y  W  YAIGGS +P++ SE N FIA   S SKE     V     N     W +
Sbjct: 286 HVANNRYDKWIMYAIGGSADPTIFSEGNYFIASDKSNSKEVTKREVKGGWNN-----WRW 345

Query: 301 HSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVA 333
            + +DVF+NGA F  +  G G ++  Y++ QRF VA
Sbjct: 346 RTSKDVFKNGAYF--VPSGYGSISLPYSSAQRFTVA 370

BLAST of Moc03g33490 vs. ExPASy TrEMBL
Match: A0A6J1CB55 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009084 PE=3 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.9e-195
Identity = 332/339 (97.94%), Postives = 333/339 (98.23%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL
Sbjct: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII
Sbjct: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
           RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDW---VVWSTGNVEETKWNFH 300
           VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE   +W   VVWSTGNVEETKWNFH
Sbjct: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEKILNWRLQVVWSTGNVEETKWNFH 300

Query: 301 SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 337
           SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW
Sbjct: 301 SVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 339

BLAST of Moc03g33490 vs. ExPASy TrEMBL
Match: A0A6J1C826 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009084 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 2.1e-194
Identity = 331/336 (98.51%), Postives = 331/336 (98.51%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL
Sbjct: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII
Sbjct: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
           RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 300
           VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE     VVWSTGNVEETKWNFHSVR
Sbjct: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKE-----VVWSTGNVEETKWNFHSVR 300

Query: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 337
           DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW
Sbjct: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 331

BLAST of Moc03g33490 vs. ExPASy TrEMBL
Match: A0A1S3AY18 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103483978 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 1.4e-177
Identity = 297/336 (88.39%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MNAIDRCWRSNPYWRRNRQQL  CSVGF GKMTNN+GRNLI YQVTDPSDDPINPRPGTL
Sbjct: 33  MNAIDRCWRSNPYWRRNRQQLATCSVGFAGKMTNNMGRNLIHYQVTDPSDDPINPRPGTL 92

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RYGA++I+ KVWITF++DM I L KPL+ISSFTAIDGRGATVHI GNACLMVLRA+NIII
Sbjct: 93  RYGATMIKHKVWITFQKDMHIVLQKPLLISSFTAIDGRGATVHITGNACLMVLRATNIII 152

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HGLIIHHCR+QAA +VMGPNSK+ISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 153 HGLIIHHCRSQAAGEVMGPNSKVISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 212

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
           RGSTDITISNNWFRDQDKVILLGHDDGYVRDR MKVTVVYNHFGPNCNQRMPRIRYGYAH
Sbjct: 213 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRNMKVTVVYNHFGPNCNQRMPRIRYGYAH 272

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 300
           VANN+YQGWTQYAIGGSMNPSVKSEANLFIASKSK+     V+WSTGNVEE KW FHSVR
Sbjct: 273 VANNLYQGWTQYAIGGSMNPSVKSEANLFIASKSKQ-----VIWSTGNVEEAKWKFHSVR 332

Query: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 337
           DVFENGA F +IGVGRG V PNYN QQRF VADAKW
Sbjct: 333 DVFENGALFTEIGVGRGRVKPNYNPQQRFPVADAKW 363

BLAST of Moc03g33490 vs. ExPASy TrEMBL
Match: A0A6J1E7R9 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431420 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 4.5e-176
Identity = 292/336 (86.90%), Postives = 316/336 (94.05%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL
Sbjct: 30  MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 89

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RYGA++I+ KVWITF++DM I LHKPL+ISS+TAIDGRGA+VHI+GNACL+V +A+NIII
Sbjct: 90  RYGATMIKSKVWITFQKDMHILLHKPLLISSYTAIDGRGASVHISGNACLVVSKATNIII 149

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HGLIIHHC+AQ A QVMGPNSK++SLGHVDGDAIRLV+ASKVWIDHNTLYRCEDGLLDVT
Sbjct: 150 HGLIIHHCKAQGAGQVMGPNSKLVSLGHVDGDAIRLVSASKVWIDHNTLYRCEDGLLDVT 209

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
           RGSTDITISNNWFRDQDKVILLGH+DGYVRD+ M+VTVVYNHFGPNCNQRMPRIR+GYAH
Sbjct: 210 RGSTDITISNNWFRDQDKVILLGHEDGYVRDKNMRVTVVYNHFGPNCNQRMPRIRHGYAH 269

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 300
           VANN+YQGWTQYAIGGSMNPSVKSEANLFIASKSK+     +VWSTG VE  KW FHSVR
Sbjct: 270 VANNLYQGWTQYAIGGSMNPSVKSEANLFIASKSKQ-----IVWSTGKVEAAKWKFHSVR 329

Query: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 337
           DVFENGASF QIGVGRG V PNY+AQQRF VADAKW
Sbjct: 330 DVFENGASFGQIGVGRGGVKPNYSAQQRFPVADAKW 360

BLAST of Moc03g33490 vs. ExPASy TrEMBL
Match: A0A0A0LPZ1 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G031910 PE=3 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 7.2e-174
Identity = 287/336 (85.42%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           MNAIDRCWRSNPYWR+NRQQL  CSVGF GKMTNN+GRNL+ YQVTDPSDDPINPRPGTL
Sbjct: 35  MNAIDRCWRSNPYWRKNRQQLATCSVGFAGKMTNNMGRNLMHYQVTDPSDDPINPRPGTL 94

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RYGA++I+ KVWITF++DM I L KPL++SSFTAIDGRG+TVHI GNACLMV+RA+NIII
Sbjct: 95  RYGATMIKHKVWITFKKDMHIVLQKPLLVSSFTAIDGRGSTVHITGNACLMVVRATNIII 154

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HGLIIHHC++QAA QVMGPN+K+ISLG+VDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT
Sbjct: 155 HGLIIHHCKSQAAGQVMGPNAKVISLGNVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 214

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
           RGSTDITISNNWFRDQDKVILLGHDDGYVRDR M+VTV+YNHFGPNCNQRMPRIRYGYAH
Sbjct: 215 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRSMRVTVLYNHFGPNCNQRMPRIRYGYAH 274

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 300
           VANN+YQGWTQYAIGGSMNPSVKSEANLFIASKSK+     V+WSTG VEE KW FHSVR
Sbjct: 275 VANNLYQGWTQYAIGGSMNPSVKSEANLFIASKSKQ-----VIWSTGKVEEAKWKFHSVR 334

Query: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVADAKW 337
           D FENGASFA+IGVG+G V PNYN QQRF VADAKW
Sbjct: 335 DAFENGASFAEIGVGKGRVKPNYNPQQRFPVADAKW 365

BLAST of Moc03g33490 vs. TAIR 10
Match: AT5G09280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 411.0 bits (1055), Expect = 1.7e-114
Identity = 183/243 (75.31%), Postives = 221/243 (90.95%), Query Frame = 0

Query: 32  MTNNIGRNLIRYQVTDPSDDPINPRPGTLRYGASVIRGKVWITFRRDMRITLHKPLMISS 91
           MT NIG+ + +Y+VTDPSDDP+NP+PGTLRYGA++++GK WITF+R+M+I LHKPL+ISS
Sbjct: 1   MTGNIGKGVTQYKVTDPSDDPLNPKPGTLRYGATLVKGKKWITFKRNMKIKLHKPLLISS 60

Query: 92  FTAIDGRGATVHIAGNACLMVLRASNIIIHGLIIHHCRAQAAAQVMGPNSKIISLGHVDG 151
           FTA+DGRGA+VHI+G ACL+V RA+++IIHGL IH C+A   + VMGP+SKII LGH+DG
Sbjct: 61  FTALDGRGASVHISGPACLIVYRATDVIIHGLKIHDCKAHPPSSVMGPDSKIIQLGHMDG 120

Query: 152 DAIRLVTASKVWIDHNTLYRCEDGLLDVTRGSTDITISNNWFRDQDKVILLGHDDGYVRD 211
           DAIRLVTA KVWIDHNTLY CEDGLLDVT GSTD+T+SNNWFR+QDKV+LLGHDDGYV+D
Sbjct: 121 DAIRLVTAKKVWIDHNTLYDCEDGLLDVTLGSTDVTVSNNWFRNQDKVMLLGHDDGYVKD 180

Query: 212 RRMKVTVVYNHFGPNCNQRMPRIRYGYAHVANNMYQGWTQYAIGGSMNPSVKSEANLFIA 271
           + M+VTVV+NHFGPNCNQRMPR+R+GYAHVANN YQGWTQYAIGGSM+P VKSE+N F+A
Sbjct: 181 KDMRVTVVFNHFGPNCNQRMPRVRHGYAHVANNYYQGWTQYAIGGSMSPRVKSESNYFVA 240

Query: 272 SKS 275
            KS
Sbjct: 241 PKS 243

BLAST of Moc03g33490 vs. TAIR 10
Match: AT3G17730.1 (NAC domain containing protein 57 )

HSP 1 Score: 375.2 bits (962), Expect = 1.0e-103
Identity = 187/249 (75.10%), Postives = 209/249 (83.94%), Query Frame = 0

Query: 351 MAPVGLPPGFRFHPTDEELVNYYLKRKISGQEIELDIIPEVDLYKCEPWELSDKSFLPSR 410
           MAPVGLPPGFRFHPTDEELVNYYLKRKI+GQEIELDIIPEVDLYKCEPW+L++KSFLPSR
Sbjct: 1   MAPVGLPPGFRFHPTDEELVNYYLKRKINGQEIELDIIPEVDLYKCEPWDLAEKSFLPSR 60

Query: 411 DPEWYFFGPRDRKYPNGFRTNRATRAGYWKSTGKDRRVTSQNRPIGMKKTLVYYRGRAPQ 470
           DPEWYFFGPRDRKYPNGFRTNRATR GYWKSTGKDRRVTSQ+R IGMKKTLVYY+GRAPQ
Sbjct: 61  DPEWYFFGPRDRKYPNGFRTNRATRGGYWKSTGKDRRVTSQSRAIGMKKTLVYYKGRAPQ 120

Query: 471 GIRTDWVMHEYRLHDNDYCDSDTSAIQDSYALCRVFKKNGICSEVEE----AGGQSSSSG 530
           GIRTDWVMHEYRL D D CD D S++QDSYALCRVFKKNGICSE+E       GQ S + 
Sbjct: 121 GIRTDWVMHEYRLDDKD-CD-DPSSLQDSYALCRVFKKNGICSELESERQLQTGQCSFTT 180

Query: 531 LSFMD-NSTSQTLMNHDYETLSPDVLMAASSSCVEE--EEKDDSWMQFITEDAWCASNSS 590
            S  + NS +    N+DYET+SP+V    SS+CVEE  ++KDDSWMQFIT+DAW  S++ 
Sbjct: 181 ASMEEINSNNNNNYNNDYETMSPEV--GVSSACVEEVVDDKDDSWMQFITDDAWDTSSNG 240

Query: 591 PIAGAGAGL 593
              G G G+
Sbjct: 241 AAMGHGQGV 245

BLAST of Moc03g33490 vs. TAIR 10
Match: AT1G11920.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 2.1e-85
Identity = 163/341 (47.80%), Postives = 214/341 (62.76%), Query Frame = 0

Query: 2   NAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTLR 61
           N ID CWR NPYW  NR+ L  C+VGF GK     G+    Y VT+PSDDP NPRPGTLR
Sbjct: 37  NPIDSCWRRNPYWASNRRALADCAVGF-GKSAVG-GKYGSIYVVTNPSDDPENPRPGTLR 96

Query: 62  YGASVIRGK-VWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 121
           Y  +VI+ K +WITF RDM I L   L+++S+  IDGRGA V IA   C+ +   S++II
Sbjct: 97  Y--AVIQSKPLWITFARDMVIVLRNELIMNSYKTIDGRGAKVEIAYGPCITIQHVSHVII 156

Query: 122 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 181
           HG+ IH C+   + +V    + + S    DGDAI +  +S +WIDH    RC+DGL+DV 
Sbjct: 157 HGISIHDCKPGKSGRVRSSPTHVGSRKGSDGDAIAIFDSSHIWIDHCFFSRCQDGLIDVL 216

Query: 182 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 241
             ST +TISNN+F   DKV+LLGH+D  V D+ M+VT+ +NHFGP   +RMPR+R GYAH
Sbjct: 217 HASTAVTISNNYFTQHDKVMLLGHNDNNVEDKIMRVTIAFNHFGPGLIERMPRVRRGYAH 276

Query: 242 VANNMYQGWTQYAIGGSMNPSVKSEANLFIAS---------KSKEARYDWVVWSTGNVEE 301
           VANN Y+ W  YAIGGS +P++ SE N F+AS         K  ++ YDW          
Sbjct: 277 VANNRYEKWQMYAIGGSADPTIFSEGNYFVASDDPSKKQVTKRIDSGYDW---------- 336

Query: 302 TKWNFHSVRDVFENGASFAQIGVGRGVVTPNYNAQQRFQVA 333
            +W + + +DVF+NGA F  +  G G VTP Y   +RF V+
Sbjct: 337 KRWKWRTSKDVFKNGAYF--VPSGYGTVTPLYGRAERFPVS 361

BLAST of Moc03g33490 vs. TAIR 10
Match: AT1G30350.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 302.4 bits (773), Expect = 8.3e-82
Identity = 159/332 (47.89%), Postives = 204/332 (61.45%), Query Frame = 0

Query: 1   MNAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDPINPRPGTL 60
           +N ID CWR NP W  NRQ L  C+VG+ GK     G+N   Y VT+PSD+P  P PGTL
Sbjct: 35  LNPIDACWRRNPKWATNRQALAHCAVGY-GKAAIG-GKNGPIYVVTNPSDNPTRPSPGTL 94

Query: 61  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 120
           RY  S  +  +WITF RDM I L   LMI+S+  IDGRGA V IA   CL + +  ++II
Sbjct: 95  RYAVSQPK-PLWITFARDMVIVLKSQLMINSYKTIDGRGAKVEIANGPCLRIRQVKHVII 154

Query: 121 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 180
           HG+ IH C+A        PN        +DGD IR+  ++ VWIDH  L RC DGL+DV 
Sbjct: 155 HGISIHDCKAD-------PNG-------MDGDGIRVFQSTHVWIDHCFLSRCHDGLIDVI 214

Query: 181 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 240
             ST +TISNN+F   DKV+LLGHDD Y+ D+ M+VT+ +N FGP   +RMPR+R GYAH
Sbjct: 215 VSSTAVTISNNYFTQHDKVMLLGHDDSYMGDKDMRVTIAFNTFGPGLIERMPRVRRGYAH 274

Query: 241 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 300
           VANN Y+ W  YAIGGS NP + SE N F+A + + ++        G  +  +W + + R
Sbjct: 275 VANNRYEKWQMYAIGGSANPIIFSEGNYFVAPEKRSSKQVTKRMMAG-PDSKRWKWGTSR 334

Query: 301 DVFENGASFAQIGVGRGVVTPNYNAQQRFQVA 333
           DVF NGA F   GV   +V P Y   + F+VA
Sbjct: 335 DVFMNGAFFGPPGV---IVRPLYKGGEGFRVA 345

BLAST of Moc03g33490 vs. TAIR 10
Match: AT3G24230.1 (Pectate lyase family protein )

HSP 1 Score: 298.1 bits (762), Expect = 1.6e-80
Identity = 151/315 (47.94%), Postives = 192/315 (60.95%), Query Frame = 0

Query: 2   NAIDRCWRSNPYWRRNRQQLGVCSVGFVGKMTNNIGRNLIRYQVTDPSDDP-INPRPGTL 61
           N ID CWR +  W +NR+ L  C +GF  K     GRN   Y VTD SDD  +NP+PGTL
Sbjct: 104 NLIDDCWRCDRNWNKNRKHLADCGMGFGSKAFG--GRNGSYYVVTDHSDDDVVNPKPGTL 163

Query: 62  RYGASVIRGKVWITFRRDMRITLHKPLMISSFTAIDGRGATVHIAGNACLMVLRASNIII 121
           R+    +   +WI F+RDM I L + L+++SF  ID RGA VHIA  AC+ +   +N+I+
Sbjct: 164 RHAVIQVE-PLWIIFKRDMVIKLKQELIMNSFKTIDARGANVHIANGACITIQNITNVIV 223

Query: 122 HGLIIHHCRAQAAAQVMGPNSKIISLGHVDGDAIRLVTASKVWIDHNTLYRCEDGLLDVT 181
           HGL IH C+      V    S+    G  DGDAI +  +S +WIDHN+L  C DGL+DV 
Sbjct: 224 HGLHIHDCKRTGNVTVRSSPSQAGFRGTADGDAINIFGSSHIWIDHNSLSNCTDGLVDVV 283

Query: 182 RGSTDITISNNWFRDQDKVILLGHDDGYVRDRRMKVTVVYNHFGPNCNQRMPRIRYGYAH 241
            GST ITISNN F   D+V+LLGH+D Y RD+ M+VTV YNHFG    QRMPR R+GY H
Sbjct: 284 NGSTAITISNNHFTHHDEVMLLGHNDSYTRDKMMQVTVAYNHFGEGLIQRMPRCRHGYFH 343

Query: 242 VANNMYQGWTQYAIGGSMNPSVKSEANLFIASKSKEARYDWVVWSTGNVEETKWNFHSVR 301
           V NN Y  W  YAIGGS NP++ S+ N F A K+  A+       T   E  +WN+ S +
Sbjct: 344 VVNNDYTHWKMYAIGGSANPTINSQGNRFAAPKNHSAKEVTKRLDTKGNEWMEWNWRSEK 403

Query: 302 DVFENGASFAQIGVG 316
           D+  NGA F   G G
Sbjct: 404 DLLVNGAFFTPSGEG 415

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6584442.13.6e-28978.66putative pectate lyase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022137718.14.0e-19597.94putative pectate lyase 2 isoform X1 [Momordica charantia][more]
XP_022137719.14.4e-19498.51putative pectate lyase 2 isoform X2 [Momordica charantia][more]
XP_008439120.12.9e-17788.39PREDICTED: putative pectate lyase 2 [Cucumis melo][more]
XP_038896018.18.4e-17787.80putative pectate lyase 2 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O653883.0e-8447.80Putative pectate lyase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g11920 PE=3 SV=2[more]
Q9C8G41.2e-8047.89Probable pectate lyase 4 OS=Arabidopsis thaliana OX=3702 GN=At1g30350 PE=2 SV=1[more]
Q9LRM52.2e-7947.94Probable pectate lyase 9 OS=Arabidopsis thaliana OX=3702 GN=At3g24230 PE=2 SV=1[more]
Q9SVQ61.9e-7847.94Putative pectate lyase 14 OS=Arabidopsis thaliana OX=3702 GN=At4g13210 PE=3 SV=2[more]
O654561.9e-7847.02Probable pectate lyase 16 OS=Arabidopsis thaliana OX=3702 GN=At4g22080 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CB551.9e-19597.94Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009084 PE=3 SV=1[more]
A0A6J1C8262.1e-19498.51Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009084 PE=3 SV=1[more]
A0A1S3AY181.4e-17788.39Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103483978 PE=3 SV=1[more]
A0A6J1E7R94.5e-17686.90Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111431420 PE=3 SV=1[more]
A0A0A0LPZ17.2e-17485.42Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G031910 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G09280.11.7e-11475.31Pectin lyase-like superfamily protein [more]
AT3G17730.11.0e-10375.10NAC domain containing protein 57 [more]
AT1G11920.12.1e-8547.80Pectin lyase-like superfamily protein [more]
AT1G30350.18.3e-8247.89Pectin lyase-like superfamily protein [more]
AT3G24230.11.6e-8047.94Pectate lyase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 150..171
score: 48.48
coord: 230..249
score: 62.62
coord: 49..74
score: 42.31
coord: 252..271
score: 58.57
coord: 294..318
score: 39.81
coord: 86..102
score: 51.82
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 78..275
e-value: 3.8E-69
score: 245.7
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 92..269
e-value: 7.4E-24
score: 84.5
IPR036093NAC domain superfamilyGENE3D2.170.150.80NAC domaincoord: 365..511
e-value: 1.6E-57
score: 195.6
IPR036093NAC domain superfamilySUPERFAMILY101941NAC domaincoord: 350..508
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 1..343
e-value: 9.9E-88
score: 296.4
IPR003441NAC domainPFAMPF02365NAMcoord: 357..483
e-value: 4.1E-39
score: 134.4
IPR003441NAC domainPROSITEPS51005NACcoord: 356..508
score: 60.070797
NoneNo IPR availablePANTHERPTHR31683:SF74PECTATE LYASEcoord: 1..335
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 1..335
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 1..321

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g33490.1Moc03g33490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003677 DNA binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity