Moc03g29380 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g29380
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description11-beta-hydroxysteroid dehydrogenase 1B-like
Locationchr3: 21012164 .. 21013629 (-)
RNA-Seq ExpressionMoc03g29380
SyntenyMoc03g29380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCTCGTTTACAGTTTCTTAAACCTCGTACTTACCACAACATCTCTAATTGCCTTCATCTTCCTTCTCCCACCATACTTGTTCTTCAAGTTCCTCTCATTCCTTCTAAGGTCCACCTTCAGCGAAGATGTCGCCGGAAAAGTCGTTCTGATCACCGGAGCATCCTCGGGCATCGGCGAGGTTCTGCCCCCACCCACCCTCTGCTTCCAATTAAAATTATAACCCAACAACAAAATAGATTTTAATAAATTAATTATTTAATTTGGGGTGTGGGTTTTGATTGTTGAAGCACCTGGCGTACGAGTACGCTAAAAGAGGAGCTTATCTAGCGTTGGCCGCCAGAAGGGAAAACCGGCTCCGGGAAGTCGCCGCCGTGGCGGAATCTCTAGGCTCACCATTTGCTCTCGTAATCCCTGCAGATGTTTCCCAGATTCAGGACTGTAAACGATGTATCGAATCCACCGTGGCCCACTTTGGACGACGTAAGTAAACGAAATTCGATTTCTTTTGTGAATATAATATAATATATATAATTGATAAAATTTGTCCCAACAGTGAATCATTTGGTGAACAACGCGGGGGTATCCAGTATCAATCTGTTTGAAGAATACGACGACCTCAACAATGCGGTCCCAATAATGGTGAAGTGACCATTAATCTTAATTTTTTAGCTCATAATTTTTCAATAGTAATTGAGGTATTGATGTTGGGGGCGCAGGATGTGAACTTCTGGGGAATGGTTTATTGCACTTATTATGCGATTCCACACCTGAAAGCAAGCAGAGGGAAGATCATCGGAATCGCTTCCTCCGCCGCGTGGCTACCTACGCCCAGGCTTAGCTTCTACAGGGTATGTGTAACGCACCTATTAATTTATGTTATAATTAATCACTTGATCCAGTTTAAATTAATAGTTTGATGAATTGAAATTGAAGGCAAGCAAAGCGGCAGTGATAAGCTTCTACGAGACACTGAGAGTTGAAGTTGGGGGAGAGATCGGAATCTCCATTATCACGCCGGGGCTGATCGAATCAGAGATGACGCGTGGGAAATTCATGTCCAAAGAAGGCCGTTTGCAATTGGATCAACAACTGAGAGACGTAAGTTAAATTAAGTTAATTAATTCCAACTGTGCATGATTTGGAGCCAGCTTTAAGCCTATAATTATTAAGGATTAATAATGAATAAACTGTCATTTAATTTATCAACGGATATCTCAAACAGTCATTTGCGTAATTCAGGTTGTGCTGAGCGTGATGCCGGTTATGCCGATCGGAGGGGCGGTGAAGGGAATCTTGAGGAGCGGTTGCCGCGGAGATGCGTATGCGACGGAGCCGTCGTGGATGAGGATGGCGTTTTACTACGCCACGCTGTGCCCGGAGTTAGTGGAGTGGCTTAATCGCTTCATTGTTATCACCGCAACTTCTTACTTCTCCACCAACCCAACTCCAATTGCCACATAA

mRNA sequence

ATGGATCTCGTTTACAGTTTCTTAAACCTCGTACTTACCACAACATCTCTAATTGCCTTCATCTTCCTTCTCCCACCATACTTGTTCTTCAAGTTCCTCTCATTCCTTCTAAGGTCCACCTTCAGCGAAGATGTCGCCGGAAAAGTCGTTCTGATCACCGGAGCATCCTCGGGCATCGGCGAGCACCTGGCGTACGAGTACGCTAAAAGAGGAGCTTATCTAGCGTTGGCCGCCAGAAGGGAAAACCGGCTCCGGGAAGTCGCCGCCGTGGCGGAATCTCTAGGCTCACCATTTGCTCTCGTAATCCCTGCAGATGTTTCCCAGATTCAGGACTGTAAACGATGTATCGAATCCACCGTGGCCCACTTTGGACGACTGAATCATTTGGTGAACAACGCGGGGGTATCCAGTATCAATCTGTTTGAAGAATACGACGACCTCAACAATGCGGATGTGAACTTCTGGGGAATGGTTTATTGCACTTATTATGCGATTCCACACCTGAAAGCAAGCAGAGGGAAGATCATCGGAATCGCTTCCTCCGCCGCGTGGCTACCTACGCCCAGGCTTAGCTTCTACAGGGCAAGCAAAGCGGCAGTGATAAGCTTCTACGAGACACTGAGAGTTGAAGTTGGGGGAGAGATCGGAATCTCCATTATCACGCCGGGGCTGATCGAATCAGAGATGACGCGTGGGAAATTCATGTCCAAAGAAGGCCGTTTGCAATTGGATCAACAACTGAGAGACGTTGTGCTGAGCGTGATGCCGGTTATGCCGATCGGAGGGGCGGTGAAGGGAATCTTGAGGAGCGGTTGCCGCGGAGATGCGTATGCGACGGAGCCGTCGTGGATGAGGATGGCGTTTTACTACGCCACGCTGTGCCCGGAGTTAGTGGAGTGGCTTAATCGCTTCATTGTTATCACCGCAACTTCTTACTTCTCCACCAACCCAACTCCAATTGCCACATAA

Coding sequence (CDS)

ATGGATCTCGTTTACAGTTTCTTAAACCTCGTACTTACCACAACATCTCTAATTGCCTTCATCTTCCTTCTCCCACCATACTTGTTCTTCAAGTTCCTCTCATTCCTTCTAAGGTCCACCTTCAGCGAAGATGTCGCCGGAAAAGTCGTTCTGATCACCGGAGCATCCTCGGGCATCGGCGAGCACCTGGCGTACGAGTACGCTAAAAGAGGAGCTTATCTAGCGTTGGCCGCCAGAAGGGAAAACCGGCTCCGGGAAGTCGCCGCCGTGGCGGAATCTCTAGGCTCACCATTTGCTCTCGTAATCCCTGCAGATGTTTCCCAGATTCAGGACTGTAAACGATGTATCGAATCCACCGTGGCCCACTTTGGACGACTGAATCATTTGGTGAACAACGCGGGGGTATCCAGTATCAATCTGTTTGAAGAATACGACGACCTCAACAATGCGGATGTGAACTTCTGGGGAATGGTTTATTGCACTTATTATGCGATTCCACACCTGAAAGCAAGCAGAGGGAAGATCATCGGAATCGCTTCCTCCGCCGCGTGGCTACCTACGCCCAGGCTTAGCTTCTACAGGGCAAGCAAAGCGGCAGTGATAAGCTTCTACGAGACACTGAGAGTTGAAGTTGGGGGAGAGATCGGAATCTCCATTATCACGCCGGGGCTGATCGAATCAGAGATGACGCGTGGGAAATTCATGTCCAAAGAAGGCCGTTTGCAATTGGATCAACAACTGAGAGACGTTGTGCTGAGCGTGATGCCGGTTATGCCGATCGGAGGGGCGGTGAAGGGAATCTTGAGGAGCGGTTGCCGCGGAGATGCGTATGCGACGGAGCCGTCGTGGATGAGGATGGCGTTTTACTACGCCACGCTGTGCCCGGAGTTAGTGGAGTGGCTTAATCGCTTCATTGTTATCACCGCAACTTCTTACTTCTCCACCAACCCAACTCCAATTGCCACATAA

Protein sequence

MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIGEHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTVAHFGRLNHLVNNAGVSSINLFEEYDDLNNADVNFWGMVYCTYYAIPHLKASRGKIIGIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMSKEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPELVEWLNRFIVITATSYFSTNPTPIAT
Homology
BLAST of Moc03g29380 vs. NCBI nr
Match: XP_022137233.1 (11-beta-hydroxysteroid dehydrogenase 1B-like [Momordica charantia])

HSP 1 Score: 623.2 bits (1606), Expect = 1.2e-174
Identity = 322/326 (98.77%), Postives = 322/326 (98.77%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG
Sbjct: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV
Sbjct: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
           AHFGRLNHLVNNAGVSSINLFEEYDDLNNA    DVNFWGMVYCTYYAIPHLKASRGKII
Sbjct: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNAVPIMDVNFWGMVYCTYYAIPHLKASRGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
           GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS
Sbjct: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE
Sbjct: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300

Query: 301 LVEWLNRFIVITATSYFSTNPTPIAT 323
           LVEWLNRFIVITATSYFSTNPTPIAT
Sbjct: 301 LVEWLNRFIVITATSYFSTNPTPIAT 326

BLAST of Moc03g29380 vs. NCBI nr
Match: XP_023542706.1 (11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 484.2 bits (1245), Expect = 8.8e-133
Identity = 246/315 (78.10%), Postives = 275/315 (87.30%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MD + + LN VL  TSLIAFIF LP YLFFKFLSF+ RS FSEDVAGKVVLITGASSGIG
Sbjct: 1   MDFLNNVLNFVLPFTSLIAFIFFLPTYLFFKFLSFVFRSIFSEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           +H+AYEYAKRGAYLAL ARRENRLREVA VA   GSPFALVIPAD+S+I+DCKRCI++TV
Sbjct: 61  KHMAYEYAKRGAYLALVARRENRLREVAGVARFFGSPFALVIPADISKIEDCKRCIQTTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
           A FGRL+HLVNNAGVSSINLFEEYD+ +NA    DVNFWGMVYCTY+AIPHLK SRGKII
Sbjct: 121 AVFGRLDHLVNNAGVSSINLFEEYDNHDNAAPIMDVNFWGMVYCTYFAIPHLKQSRGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
           GI+SSAAWLP PRLSFY ASKAAVISFYETLRVEVG +IGI+I+TPGL+ESEMT+GKFMS
Sbjct: 181 GISSSAAWLPVPRLSFYSASKAAVISFYETLRVEVGRDIGITIVTPGLVESEMTQGKFMS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           K+G L LDQ+LRD ++S MP+MPI  AVKGI+RS CRGD YATEP WMRMAFYY TL PE
Sbjct: 241 KDGGLYLDQELRDAMVSAMPIMPIDSAVKGIVRSACRGDRYATEPRWMRMAFYYKTLWPE 300

Query: 301 LVEWLNRFIVITATS 312
           LVEW+N  I +T +S
Sbjct: 301 LVEWVNYLIGMTGSS 315

BLAST of Moc03g29380 vs. NCBI nr
Match: XP_008446283.1 (PREDICTED: 11-beta-hydroxysteroid dehydrogenase 1B-like [Cucumis melo])

HSP 1 Score: 482.3 bits (1240), Expect = 3.4e-132
Identity = 244/315 (77.46%), Postives = 274/315 (86.98%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MD + +FLNL+L  TSL++F F LP YLFFK LS ++RS FSEDVAGKVVLITGASSGIG
Sbjct: 1   MDPLNNFLNLILPPTSLVSFFFFLPIYLFFKSLSSIIRSIFSEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           EHLAYEYAKRGAYLAL ARRENRLREVAAVA+ LGSP+ALVIPAD+S+I+DCKRCI++TV
Sbjct: 61  EHLAYEYAKRGAYLALVARRENRLREVAAVAQYLGSPYALVIPADISKIEDCKRCIQTTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
            HF RL+HLVNNAGVSS+NLFEEYD+L NA    DVNFWGMVYC+YY IPHLK SRGKII
Sbjct: 121 THFRRLDHLVNNAGVSSVNLFEEYDNLQNAVPVMDVNFWGMVYCSYYGIPHLKQSRGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
           GIASSAAWLP PRLSFY +SKAAVISFYETLRVEVG +IGI+I+TPGL+ESEMT+GKF+S
Sbjct: 181 GIASSAAWLPAPRLSFYTSSKAAVISFYETLRVEVGRDIGITIVTPGLVESEMTQGKFIS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           K+GRL LDQQLRD  +S MP+MPI  AVKGILRS CRGD YATEP WMRMAFYY TL PE
Sbjct: 241 KDGRLYLDQQLRDATVSAMPIMPIDDAVKGILRSVCRGDRYATEPRWMRMAFYYKTLWPE 300

Query: 301 LVEWLNRFIVITATS 312
           LVEW N  I +  +S
Sbjct: 301 LVEWFNYLINMRGSS 315

BLAST of Moc03g29380 vs. NCBI nr
Match: KAG6573143.1 (11-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 481.1 bits (1237), Expect = 7.5e-132
Identity = 244/315 (77.46%), Postives = 275/315 (87.30%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MD + + LN VL  TSLIAFIF LP YLFFKFLSF+ RS FSEDVAGKVVLITGASSGIG
Sbjct: 1   MDFLNNVLNFVLPITSLIAFIFFLPTYLFFKFLSFVFRSIFSEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           +H+AYEYAKRGAYLAL ARRENRLREVA VA   GSPFALVIPAD+S+I+DCKRCI++TV
Sbjct: 61  KHMAYEYAKRGAYLALVARRENRLREVAGVARFFGSPFALVIPADISRIEDCKRCIQTTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
           A FGRL+HLVNNAGVSSINLFEEY++ +NA    DVNFWGMVYCTY+AIPHLK SRGKII
Sbjct: 121 AVFGRLDHLVNNAGVSSINLFEEYNNHDNAAPIMDVNFWGMVYCTYFAIPHLKQSRGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
           GI+SSAAWLP PRLSFY ASKAAVISFYETLRVEVG +IGI+I+TPGL+ESEMT+GKFMS
Sbjct: 181 GISSSAAWLPVPRLSFYSASKAAVISFYETLRVEVGRDIGITIVTPGLVESEMTQGKFMS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           K+G L LDQ+LRD ++S MP+MPI  AV GI+RS CRGD YATEP WMRMAFYY TL PE
Sbjct: 241 KDGGLYLDQELRDAMVSAMPIMPIDSAVNGIVRSACRGDRYATEPRWMRMAFYYKTLWPE 300

Query: 301 LVEWLNRFIVITATS 312
           LVEW+N  I +T++S
Sbjct: 301 LVEWVNYLIGMTSSS 315

BLAST of Moc03g29380 vs. NCBI nr
Match: XP_022955046.1 (11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita moschata])

HSP 1 Score: 479.6 bits (1233), Expect = 2.2e-131
Identity = 244/315 (77.46%), Postives = 273/315 (86.67%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MD + + LN VL  TSLIAFIF LP YLFFKFLSF+ RS FSEDVAGKVVLITGASSGIG
Sbjct: 1   MDFLNNVLNFVLPITSLIAFIFFLPTYLFFKFLSFVFRSIFSEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           +H+AYEYAKRGAYL L ARRENRLREVA VA   GSPFALVIPAD+S+I+DCKRCI++TV
Sbjct: 61  KHMAYEYAKRGAYLVLVARRENRLREVAGVARFFGSPFALVIPADISRIEDCKRCIQTTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
           A FGRL+HLVNNAGVSSINLFEEY++ +NA    DVNFWGMVYCTY+AIPHLK SRGKII
Sbjct: 121 AVFGRLDHLVNNAGVSSINLFEEYNNHDNAAPIMDVNFWGMVYCTYFAIPHLKQSRGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
           GI+SSAAWLP PRLSFY ASKAAVISFYETLRVEVG +IGI+I+TPGLIESEMT+GKFMS
Sbjct: 181 GISSSAAWLPVPRLSFYSASKAAVISFYETLRVEVGRDIGITIVTPGLIESEMTQGKFMS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           K+G L LDQ+LRD ++S MP+MPI  AV GI+RS CRGD YATEP WMRMAFYY TL PE
Sbjct: 241 KDGGLYLDQELRDAMVSAMPIMPIDSAVNGIVRSACRGDRYATEPRWMRMAFYYKTLWPE 300

Query: 301 LVEWLNRFIVITATS 312
           LVEW+N  I +T +S
Sbjct: 301 LVEWVNYLIGMTGSS 315

BLAST of Moc03g29380 vs. ExPASy Swiss-Prot
Match: P0DKC5 (11-beta-hydroxysteroid dehydrogenase 1A OS=Arabidopsis thaliana OX=3702 GN=HSD1 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.3e-91
Identity = 165/314 (52.55%), Postives = 221/314 (70.38%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           M+L+  FLNL     +     F LPP+ FFKFL  +  + FSE++ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           E LAYEYA RGA LAL ARR+NRL EVA +A  LGSP  + + ADVS+  DC+R ++ T+
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
            HFGRL+HLVNNAG++ I++FE  +D+       D NFWG VY T  A+P+L+ S GKI+
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
            ++SSAAWL  PR+SFY ASKAA++SF+ET+R+E+GG++ I+I+TPG IESE+T+GK+ S
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
            EG L ++Q +RDV +   PV    G  K I+   CR   Y TEPSW ++ + +  LCPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 LVEWLNRFIVITAT 311
           L+EW  R + +T T
Sbjct: 301 LIEWGCRLLYMTGT 314

BLAST of Moc03g29380 vs. ExPASy Swiss-Prot
Match: P0DKC6 (11-beta-hydroxysteroid dehydrogenase 1B OS=Arabidopsis thaliana OX=3702 GN=HSD1 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.3e-91
Identity = 165/314 (52.55%), Postives = 221/314 (70.38%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           M+L+  FLNL     +     F LPP+ FFKFL  +  + FSE++ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           E LAYEYA RGA LAL ARR+NRL EVA +A  LGSP  + + ADVS+  DC+R ++ T+
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
            HFGRL+HLVNNAG++ I++FE  +D+       D NFWG VY T  A+P+L+ S GKI+
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
            ++SSAAWL  PR+SFY ASKAA++SF+ET+R+E+GG++ I+I+TPG IESE+T+GK+ S
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
            EG L ++Q +RDV +   PV    G  K I+   CR   Y TEPSW ++ + +  LCPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 LVEWLNRFIVITAT 311
           L+EW  R + +T T
Sbjct: 301 LIEWGCRLLYMTGT 314

BLAST of Moc03g29380 vs. ExPASy Swiss-Prot
Match: Q93W57 (11-beta-hydroxysteroid dehydrogenase A OS=Sesamum indicum OX=4182 GN=SOP2 PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 6.7e-91
Identity = 162/313 (51.76%), Postives = 230/313 (73.48%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MDL+++FLNL+    +    +F LPP+  FKF   +L + FSEDVAGKVV+ITGASSGIG
Sbjct: 1   MDLIHTFLNLIAPPFTFFFLLFFLPPFQIFKFFLSILGTLFSEDVAGKVVVITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           E LAYEYAKRGA L LAARRE  L+EVA  A  LGSP  +V+ ADVS+ +DC++ ++ T+
Sbjct: 61  ESLAYEYAKRGACLVLAARRERSLQEVAERARDLGSPDVVVVRADVSKAEDCRKVVDQTM 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDL----NNADVNFWGMVYCTYYAIPHLKASRGKII 180
             FGRL+HLVNNAG+ S+++ EE +D+       D+NFWG VY T +A P+L+ SRG+I+
Sbjct: 121 NRFGRLDHLVNNAGIMSVSMLEEVEDITGYRETMDINFWGYVYMTRFAAPYLRNSRGRIV 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
            ++SS++W+PTPR+SFY ASKAA+  F+ETLRVE G +IGI+++TPG IESE+T+GKF +
Sbjct: 181 VLSSSSSWMPTPRMSFYNASKAAISQFFETLRVEFGPDIGITLVTPGFIESELTQGKFYN 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
             G   +DQ +RDV +S  P++ +  A + I+RS  RG+ Y TEP+W R+ +++   CPE
Sbjct: 241 -AGERVIDQDMRDVQVSTTPILRVESAARSIVRSAIRGERYVTEPAWFRVTYWWKLFCPE 300

Query: 301 LVEWLNRFIVITA 310
           ++EW+ R + + +
Sbjct: 301 VMEWVFRLMYLAS 312

BLAST of Moc03g29380 vs. ExPASy Swiss-Prot
Match: A7LB60 (11-beta-hydroxysteroid dehydrogenase A OS=Arachis hypogaea OX=3818 GN=STO-A PE=1 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.5e-90
Identity = 174/313 (55.59%), Postives = 220/313 (70.29%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MDL+++FLNLV    +       LPPY   KF+  +L    SE+VAGKVV ITGASSGIG
Sbjct: 1   MDLIHTFLNLVAPPFTFFFLCLFLPPYWGLKFMVSILSWLLSENVAGKVVHITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           E+LAYEYAKRGA LAL+ARRE  L +VA  A  LGSP  +V+ ADVS+ +DC R I+ TV
Sbjct: 61  EYLAYEYAKRGACLALSARRETALHQVADTARHLGSPDVIVMRADVSKPEDCMRLIDQTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNN----ADVNFWGMVYCTYYAIPHLKASRGKII 180
            HFGRL+HLVNNA +S   LFEE  D++N     + NFWG VY T YA+ HL+ SRGKI+
Sbjct: 121 NHFGRLDHLVNNAAISIATLFEETPDISNLRPIMETNFWGSVYTTRYALQHLRKSRGKIV 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
            ++S  +WLP PR   Y ASKAA++S YETLRVEVG EIGI+I+TPG IESE+T+GKF+S
Sbjct: 181 VMSSVDSWLPAPRRHIYSASKAALVSLYETLRVEVGSEIGITIVTPGYIESEITKGKFLS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
            +G + +DQ LRDV +S +PV  + G  + I++S  RGD   T PSW RM +    LCPE
Sbjct: 241 AQGEVDVDQDLRDVEVSAVPVGSVSGCAESIIKSTLRGDRCLTVPSWFRMTYLIKLLCPE 300

Query: 301 LVEWLNRFIVITA 310
           L+EW  R + +TA
Sbjct: 301 LLEWTFRLLYLTA 313

BLAST of Moc03g29380 vs. ExPASy Swiss-Prot
Match: Q9LUE4 (11-beta-hydroxysteroid dehydrogenase-like 6 OS=Arabidopsis thaliana OX=3702 GN=HSD6 PE=2 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.6e-84
Identity = 160/316 (50.63%), Postives = 216/316 (68.35%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MD +   +N +    +L A +   P Y   K    + R+ FSE+VAGKVV+ITGA+SGIG
Sbjct: 1   MDSINKIINFLFPLLTLYALLVFYPTYQRLKSAVSICRNLFSENVAGKVVVITGAASGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           E LAYEY KRGAYLAL   R   L  VAA+AE  GSP  L + ADVS++QDC+R I +TV
Sbjct: 61  EALAYEYGKRGAYLALVDIRGEPLFHVAALAELYGSPEVLPLVADVSKLQDCERFIRATV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
            HFGRL+HLV NAGV+ +  F + +D++ A    D+NFWG VYCT++A P+LK  RG+I+
Sbjct: 121 LHFGRLDHLVTNAGVAPLYFFADIEDVSKASPAMDINFWGSVYCTFFASPYLKKFRGRIV 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
            IAS   ++ +PRLSFY ASKAAVI+FYETLR E G +IG++I+ PG+++SEM+RGKFM+
Sbjct: 181 VIASGCGYIASPRLSFYCASKAAVIAFYETLRTEFGSDIGVTIVAPGIVDSEMSRGKFMT 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           K+G+L +D++LRDV +SV+PV       K I+RS CRGD Y  EP W+         C E
Sbjct: 241 KDGKLVVDKELRDVQMSVLPVESAERCAKAIMRSVCRGDRYLLEPDWIGCVILLKVFCSE 300

Query: 301 LVEWLNRFIVITATSY 313
             EW+ R+++I    +
Sbjct: 301 ATEWVARWLLIARPKF 316

BLAST of Moc03g29380 vs. ExPASy TrEMBL
Match: A0A6J1C9Q9 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Momordica charantia OX=3673 GN=LOC111008745 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 5.9e-175
Identity = 322/326 (98.77%), Postives = 322/326 (98.77%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG
Sbjct: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV
Sbjct: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
           AHFGRLNHLVNNAGVSSINLFEEYDDLNNA    DVNFWGMVYCTYYAIPHLKASRGKII
Sbjct: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNAVPIMDVNFWGMVYCTYYAIPHLKASRGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
           GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS
Sbjct: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE
Sbjct: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300

Query: 301 LVEWLNRFIVITATSYFSTNPTPIAT 323
           LVEWLNRFIVITATSYFSTNPTPIAT
Sbjct: 301 LVEWLNRFIVITATSYFSTNPTPIAT 326

BLAST of Moc03g29380 vs. ExPASy TrEMBL
Match: A0A0A0LR89 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G051680 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 7.3e-133
Identity = 245/320 (76.56%), Postives = 276/320 (86.25%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MDL+ +FLNL+L  TSLI+F F LP YLFFK LS ++RS FSEDVAGKVVLITGASSGIG
Sbjct: 1   MDLLNNFLNLILPPTSLISFFFFLPIYLFFKSLSSIIRSVFSEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           EHLAYEYAKRGAYLAL ARRENRLREVAAVA+ LGSP+ALVIPAD+S+I+DCKRCI++TV
Sbjct: 61  EHLAYEYAKRGAYLALVARRENRLREVAAVAQYLGSPYALVIPADISKIEDCKRCIQTTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
            HF RL+HLVNNAGVSS+NLFEEYD+L NA    DVNFWGMVYC+YY IPHLK SRGKII
Sbjct: 121 THFRRLDHLVNNAGVSSVNLFEEYDNLQNAVPVMDVNFWGMVYCSYYGIPHLKQSRGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
           GIASSAAWLP PRLSFY +SKAAVISFYETLRVEVG +IGI+I+TPGL+ESEMT+GKFMS
Sbjct: 181 GIASSAAWLPAPRLSFYTSSKAAVISFYETLRVEVGRDIGITIVTPGLVESEMTQGKFMS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           K+G L LDQQLRD  +S MP+MPI  AVKGILRS C+GD YATEP WM+MAFYY TL PE
Sbjct: 241 KDGHLYLDQQLRDATVSAMPIMPIDDAVKGILRSVCKGDRYATEPRWMKMAFYYKTLWPE 300

Query: 301 LVEWLNRFIVITATSYFSTN 317
           LVEW N  I +  +S   T+
Sbjct: 301 LVEWFNYLINMRGSSNSPTD 320

BLAST of Moc03g29380 vs. ExPASy TrEMBL
Match: A0A1S3BFD1 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucumis melo OX=3656 GN=LOC103489056 PE=3 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.6e-132
Identity = 244/315 (77.46%), Postives = 274/315 (86.98%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MD + +FLNL+L  TSL++F F LP YLFFK LS ++RS FSEDVAGKVVLITGASSGIG
Sbjct: 1   MDPLNNFLNLILPPTSLVSFFFFLPIYLFFKSLSSIIRSIFSEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           EHLAYEYAKRGAYLAL ARRENRLREVAAVA+ LGSP+ALVIPAD+S+I+DCKRCI++TV
Sbjct: 61  EHLAYEYAKRGAYLALVARRENRLREVAAVAQYLGSPYALVIPADISKIEDCKRCIQTTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
            HF RL+HLVNNAGVSS+NLFEEYD+L NA    DVNFWGMVYC+YY IPHLK SRGKII
Sbjct: 121 THFRRLDHLVNNAGVSSVNLFEEYDNLQNAVPVMDVNFWGMVYCSYYGIPHLKQSRGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
           GIASSAAWLP PRLSFY +SKAAVISFYETLRVEVG +IGI+I+TPGL+ESEMT+GKF+S
Sbjct: 181 GIASSAAWLPAPRLSFYTSSKAAVISFYETLRVEVGRDIGITIVTPGLVESEMTQGKFIS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           K+GRL LDQQLRD  +S MP+MPI  AVKGILRS CRGD YATEP WMRMAFYY TL PE
Sbjct: 241 KDGRLYLDQQLRDATVSAMPIMPIDDAVKGILRSVCRGDRYATEPRWMRMAFYYKTLWPE 300

Query: 301 LVEWLNRFIVITATS 312
           LVEW N  I +  +S
Sbjct: 301 LVEWFNYLINMRGSS 315

BLAST of Moc03g29380 vs. ExPASy TrEMBL
Match: A0A6J1GSM5 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LOC111457123 PE=3 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 1.1e-131
Identity = 244/315 (77.46%), Postives = 273/315 (86.67%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MD + + LN VL  TSLIAFIF LP YLFFKFLSF+ RS FSEDVAGKVVLITGASSGIG
Sbjct: 1   MDFLNNVLNFVLPITSLIAFIFFLPTYLFFKFLSFVFRSIFSEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           +H+AYEYAKRGAYL L ARRENRLREVA VA   GSPFALVIPAD+S+I+DCKRCI++TV
Sbjct: 61  KHMAYEYAKRGAYLVLVARRENRLREVAGVARFFGSPFALVIPADISRIEDCKRCIQTTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
           A FGRL+HLVNNAGVSSINLFEEY++ +NA    DVNFWGMVYCTY+AIPHLK SRGKII
Sbjct: 121 AVFGRLDHLVNNAGVSSINLFEEYNNHDNAAPIMDVNFWGMVYCTYFAIPHLKQSRGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
           GI+SSAAWLP PRLSFY ASKAAVISFYETLRVEVG +IGI+I+TPGLIESEMT+GKFMS
Sbjct: 181 GISSSAAWLPVPRLSFYSASKAAVISFYETLRVEVGRDIGITIVTPGLIESEMTQGKFMS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           K+G L LDQ+LRD ++S MP+MPI  AV GI+RS CRGD YATEP WMRMAFYY TL PE
Sbjct: 241 KDGGLYLDQELRDAMVSAMPIMPIDSAVNGIVRSACRGDRYATEPRWMRMAFYYKTLWPE 300

Query: 301 LVEWLNRFIVITATS 312
           LVEW+N  I +T +S
Sbjct: 301 LVEWVNYLIGMTGSS 315

BLAST of Moc03g29380 vs. ExPASy TrEMBL
Match: A0A6J1K541 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111490142 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.4e-131
Identity = 244/315 (77.46%), Postives = 273/315 (86.67%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MD + + LN VL  TSLIAFIF LP YLFFKFLSF+ RS FSE+VAGKVVLITGASSGIG
Sbjct: 1   MDFLNNVLNFVLPFTSLIAFIFFLPTYLFFKFLSFIFRSIFSENVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           +H+AYEYAKRGAYLAL ARRENRLREVA VA   GSPFALVIPAD+S+I DCKRCI++TV
Sbjct: 61  KHMAYEYAKRGAYLALVARRENRLREVAGVARFFGSPFALVIPADISRIDDCKRCIQTTV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
             FGRL+HLVNNAGVSSINLFEEYD+ +NA    DVNFWGMVYCTY+AIPHLK SRGKII
Sbjct: 121 DVFGRLDHLVNNAGVSSINLFEEYDNHDNAAPIMDVNFWGMVYCTYFAIPHLKQSRGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
           GI+SSAAWLP PRLSFY ASKAAVISFYETLRVEVG +IGI+I+TPGL+ESEMT+GKFMS
Sbjct: 181 GISSSAAWLPVPRLSFYSASKAAVISFYETLRVEVGRDIGITIVTPGLVESEMTQGKFMS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           K+G L LDQ+LRD ++S MP+MPI  AVKGI+RS CRGD YATEP WMRMAFYY TL PE
Sbjct: 241 KDGGLYLDQELRDAMVSAMPIMPIESAVKGIVRSACRGDRYATEPRWMRMAFYYKTLWPE 300

Query: 301 LVEWLNRFIVITATS 312
           LVEW+N  I +T +S
Sbjct: 301 LVEWVNYLIGMTGSS 315

BLAST of Moc03g29380 vs. TAIR 10
Match: AT5G50600.1 (hydroxysteroid dehydrogenase 1 )

HSP 1 Score: 337.0 bits (863), Expect = 1.6e-92
Identity = 165/314 (52.55%), Postives = 221/314 (70.38%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           M+L+  FLNL     +     F LPP+ FFKFL  +  + FSE++ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           E LAYEYA RGA LAL ARR+NRL EVA +A  LGSP  + + ADVS+  DC+R ++ T+
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
            HFGRL+HLVNNAG++ I++FE  +D+       D NFWG VY T  A+P+L+ S GKI+
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
            ++SSAAWL  PR+SFY ASKAA++SF+ET+R+E+GG++ I+I+TPG IESE+T+GK+ S
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
            EG L ++Q +RDV +   PV    G  K I+   CR   Y TEPSW ++ + +  LCPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 LVEWLNRFIVITAT 311
           L+EW  R + +T T
Sbjct: 301 LIEWGCRLLYMTGT 314

BLAST of Moc03g29380 vs. TAIR 10
Match: AT5G50700.1 (hydroxysteroid dehydrogenase 1 )

HSP 1 Score: 337.0 bits (863), Expect = 1.6e-92
Identity = 165/314 (52.55%), Postives = 221/314 (70.38%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           M+L+  FLNL     +     F LPP+ FFKFL  +  + FSE++ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           E LAYEYA RGA LAL ARR+NRL EVA +A  LGSP  + + ADVS+  DC+R ++ T+
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
            HFGRL+HLVNNAG++ I++FE  +D+       D NFWG VY T  A+P+L+ S GKI+
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
            ++SSAAWL  PR+SFY ASKAA++SF+ET+R+E+GG++ I+I+TPG IESE+T+GK+ S
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
            EG L ++Q +RDV +   PV    G  K I+   CR   Y TEPSW ++ + +  LCPE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 LVEWLNRFIVITAT 311
           L+EW  R + +T T
Sbjct: 301 LIEWGCRLLYMTGT 314

BLAST of Moc03g29380 vs. TAIR 10
Match: AT5G50770.1 (hydroxysteroid dehydrogenase 6 )

HSP 1 Score: 312.8 bits (800), Expect = 3.3e-85
Identity = 160/316 (50.63%), Postives = 216/316 (68.35%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MD +   +N +    +L A +   P Y   K    + R+ FSE+VAGKVV+ITGA+SGIG
Sbjct: 1   MDSINKIINFLFPLLTLYALLVFYPTYQRLKSAVSICRNLFSENVAGKVVVITGAASGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           E LAYEY KRGAYLAL   R   L  VAA+AE  GSP  L + ADVS++QDC+R I +TV
Sbjct: 61  EALAYEYGKRGAYLALVDIRGEPLFHVAALAELYGSPEVLPLVADVSKLQDCERFIRATV 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNNA----DVNFWGMVYCTYYAIPHLKASRGKII 180
            HFGRL+HLV NAGV+ +  F + +D++ A    D+NFWG VYCT++A P+LK  RG+I+
Sbjct: 121 LHFGRLDHLVTNAGVAPLYFFADIEDVSKASPAMDINFWGSVYCTFFASPYLKKFRGRIV 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
            IAS   ++ +PRLSFY ASKAAVI+FYETLR E G +IG++I+ PG+++SEM+RGKFM+
Sbjct: 181 VIASGCGYIASPRLSFYCASKAAVIAFYETLRTEFGSDIGVTIVAPGIVDSEMSRGKFMT 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 300
           K+G+L +D++LRDV +SV+PV       K I+RS CRGD Y  EP W+         C E
Sbjct: 241 KDGKLVVDKELRDVQMSVLPVESAERCAKAIMRSVCRGDRYLLEPDWIGCVILLKVFCSE 300

Query: 301 LVEWLNRFIVITATSY 313
             EW+ R+++I    +
Sbjct: 301 ATEWVARWLLIARPKF 316

BLAST of Moc03g29380 vs. TAIR 10
Match: AT5G50590.1 (hydroxysteroid dehydrogenase 4 )

HSP 1 Score: 269.2 bits (687), Expect = 4.2e-72
Identity = 138/302 (45.70%), Postives = 196/302 (64.90%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MDL     N++L   ++   +  +P  +FFK L F+     SE V GKVV+ITG+SSGIG
Sbjct: 1   MDLNNKIFNILLPIVTVSFLLVFMPFSIFFKLLQFIRGCKESEKVNGKVVIITGSSSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           EHLAYEYA+RGAYL L ARRE+RL+ VA     LGSP   V+  DVS I+DCKR ++ T+
Sbjct: 61  EHLAYEYARRGAYLTLVARREDRLQVVADRCRKLGSPDVAVVRGDVSVIKDCKRFVQETI 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNN----ADVNFWGMVYCTYYAIPHLKASRGKII 180
           + FGRL+HLVNNAG++    FE+Y ++++     + NFWG VY T++AIPHLK ++GKII
Sbjct: 121 SRFGRLDHLVNNAGIAEAKFFEDYSEISDVLPIVNTNFWGPVYATHFAIPHLKKTKGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
            +AS A W   PR+S Y ASKAA+I+FYETLR+E+  E+G++I+ PGLIE+  T    ++
Sbjct: 181 AVASPAGWSGVPRMSIYAASKAAMINFYETLRIELHPEVGVTIVFPGLIENGNTNPDLLA 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 299
           ++               V+ +       K ++   CRG  +  EPSW+R+ F+ + +CPE
Sbjct: 241 EKQDWS----------QVVTIESAAECAKAVVNGICRGKTFVAEPSWVRVLFWLSAICPE 292

BLAST of Moc03g29380 vs. TAIR 10
Match: AT5G50690.1 (hydroxysteroid dehydrogenase 7 )

HSP 1 Score: 269.2 bits (687), Expect = 4.2e-72
Identity = 138/302 (45.70%), Postives = 196/302 (64.90%), Query Frame = 0

Query: 1   MDLVYSFLNLVLTTTSLIAFIFLLPPYLFFKFLSFLLRSTFSEDVAGKVVLITGASSGIG 60
           MDL     N++L   ++   +  +P  +FFK L F+     SE V GKVV+ITG+SSGIG
Sbjct: 1   MDLNNKIFNILLPIVTVSFLLVFMPFSIFFKLLQFIRGCKESEKVNGKVVIITGSSSGIG 60

Query: 61  EHLAYEYAKRGAYLALAARRENRLREVAAVAESLGSPFALVIPADVSQIQDCKRCIESTV 120
           EHLAYEYA+RGAYL L ARRE+RL+ VA     LGSP   V+  DVS I+DCKR ++ T+
Sbjct: 61  EHLAYEYARRGAYLTLVARREDRLQVVADRCRKLGSPDVAVVRGDVSVIKDCKRFVQETI 120

Query: 121 AHFGRLNHLVNNAGVSSINLFEEYDDLNN----ADVNFWGMVYCTYYAIPHLKASRGKII 180
           + FGRL+HLVNNAG++    FE+Y ++++     + NFWG VY T++AIPHLK ++GKII
Sbjct: 121 SRFGRLDHLVNNAGIAEAKFFEDYSEISDVLPIVNTNFWGPVYATHFAIPHLKKTKGKII 180

Query: 181 GIASSAAWLPTPRLSFYRASKAAVISFYETLRVEVGGEIGISIITPGLIESEMTRGKFMS 240
            +AS A W   PR+S Y ASKAA+I+FYETLR+E+  E+G++I+ PGLIE+  T    ++
Sbjct: 181 AVASPAGWSGVPRMSIYAASKAAMINFYETLRIELHPEVGVTIVFPGLIENGNTNPDLLA 240

Query: 241 KEGRLQLDQQLRDVVLSVMPVMPIGGAVKGILRSGCRGDAYATEPSWMRMAFYYATLCPE 299
           ++               V+ +       K ++   CRG  +  EPSW+R+ F+ + +CPE
Sbjct: 241 EKQDWS----------QVVTIESAAECAKAVVNGICRGKTFVAEPSWVRVLFWLSAICPE 292

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137233.11.2e-17498.7711-beta-hydroxysteroid dehydrogenase 1B-like [Momordica charantia][more]
XP_023542706.18.8e-13378.1011-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita pepo subsp. pepo][more]
XP_008446283.13.4e-13277.46PREDICTED: 11-beta-hydroxysteroid dehydrogenase 1B-like [Cucumis melo][more]
KAG6573143.17.5e-13277.4611-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022955046.12.2e-13177.4611-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P0DKC52.3e-9152.5511-beta-hydroxysteroid dehydrogenase 1A OS=Arabidopsis thaliana OX=3702 GN=HSD1 ... [more]
P0DKC62.3e-9152.5511-beta-hydroxysteroid dehydrogenase 1B OS=Arabidopsis thaliana OX=3702 GN=HSD1 ... [more]
Q93W576.7e-9151.7611-beta-hydroxysteroid dehydrogenase A OS=Sesamum indicum OX=4182 GN=SOP2 PE=1 S... [more]
A7LB602.5e-9055.5911-beta-hydroxysteroid dehydrogenase A OS=Arachis hypogaea OX=3818 GN=STO-A PE=1... [more]
Q9LUE44.6e-8450.6311-beta-hydroxysteroid dehydrogenase-like 6 OS=Arabidopsis thaliana OX=3702 GN=H... [more]
Match NameE-valueIdentityDescription
A0A6J1C9Q95.9e-17598.7711-beta-hydroxysteroid dehydrogenase 1B-like OS=Momordica charantia OX=3673 GN=L... [more]
A0A0A0LR897.3e-13376.56Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G051680 PE=3 SV=1[more]
A0A1S3BFD11.6e-13277.4611-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1GSM51.1e-13177.4611-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1K5411.4e-13177.4611-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G50600.11.6e-9252.55hydroxysteroid dehydrogenase 1 [more]
AT5G50700.11.6e-9252.55hydroxysteroid dehydrogenase 1 [more]
AT5G50770.13.3e-8550.63hydroxysteroid dehydrogenase 6 [more]
AT5G50590.14.2e-7245.70hydroxysteroid dehydrogenase 4 [more]
AT5G50690.14.2e-7245.70hydroxysteroid dehydrogenase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 124..135
score: 50.12
coord: 173..181
score: 44.89
coord: 193..212
score: 37.17
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 49..66
score: 45.35
coord: 124..135
score: 55.08
coord: 167..183
score: 32.52
coord: 193..212
score: 34.37
coord: 213..230
score: 24.73
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 48..233
e-value: 8.6E-43
score: 146.1
NoneNo IPR availableGENE3D3.40.50.720coord: 36..310
e-value: 1.0E-60
score: 207.2
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 51..250
e-value: 1.7E-16
score: 58.1
NoneNo IPR availablePANTHERPTHR43391RETINOL DEHYDROGENASE-RELATEDcoord: 1..311
NoneNo IPR availablePANTHERPTHR43391:SF6811-BETA-HYDROXYSTEROID DEHYDROGENASE 1B-LIKE ISOFORM X1coord: 1..311
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 43..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g29380.1Moc03g29380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016491 oxidoreductase activity