Sed0020106 (gene) Chayote v1

Overview


Name	Sed0020106
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Auxin-responsive GH3 family protein
Location	LG02: 46937820 .. 46940903 (-)
RNA-Seq Expression	Sed0020106
Synteny	Sed0020106

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGAAACAGAATCCCTCCACACAGAAAAAAAAAACAAAAAACTTGCACAATCAAATTGTCAGTTTATGGTTATAGTTATGAGCTGAATTCAAGAAAAAAACAGCTTTGATGCCATGTTGTAAAACTAAACTTTTGTGTTCAAATCCCAAACTCTTTCAGTTCAAACCCCTCTTTTGTGTTCTTGATTTCTTAGATATGTTGCCACAATTTGATCCCAACAACAATGAAGATGGGTTGAAGATTCTTGAGGATCTGACTGAAAATGCAGGGCAAATACAAGAGCAGGTTATTCAGAAGATTCTTGCACAAAACTTAAACACTGAATATCTCAAAAACTTCATCAATGGCGACTCTGCTGACGTGGAACTTTTCAAGAAATCTGTCCCTGTGGTCAACTATGAAGATATCAAGCCTTATGTTGAGAGAATTGCCAATGGGGAGCCTTCCCACATAATTTCAGCTCAACCCATCACCGAGCTGCTCACAAGGTAAGAACTTTGCTTATCGACCGCGTCCATCCCAAGGTTGAGACCCGAGAGACAATACGAGATTCTACAATTCAGCTCACTCATGGATTCGAATCTGGGATCCTTGAAGGTCCCAAGTCTCTACCAACGAGGCTGTCTCTTGGGGACCAGTTTATGGACCGCGTCCATTCCAAGACCTGAATTGGGAAGACAACATGAGATTTTACAATTAACTCACTCGGGGTTTTGTAATTAGGATCTTCAAGGTTTTGAAGATCCCAAGCCTCTACCAACGAGGCGGGCCGCTGAGGACATGGTTATTGTTCATTCTTACTCTTTTCTACTTTTTTAAAGGTTTCTAAATGTGTTGAAATTACATGCAGCTCTGGAACATCTGGAGGACAGCCAAAGATGATGCCTTCTACAGAAGAAGATTTGGACAGAAAGACATTCTTTTACAACCTCCTCATCCCAGTGATGAACAAGTAAACAAACCCCTTCATGAATAAAACAGTGCAGCAGTTTTTTGTTTTGTTTAAGGTTTTTTGTTTATGTTTTTGGTGATCAAATTCACAGGTATGTGGATGGATTAGAGGAAGGAAAAGGGATGTACCTATTGTTTATAAAGCCAGAAATCAGCACCCCATCAGGGTTAATGGCAAGGCCAGTTCTCACAAGCTATTACAAAAGCAAAAACTTCACAAACAGACTATTCAATCAGTACAATGTTTACACAAGCCCCAATGAGACAATTTTGTGCTCTGACAGCCGACAGAGCATGTACTGCCAATTGCTCTGTGGGTTAGTCCAAAGAGACCATGTTCTAAGAGTTGGTGCTGTTTTTGCCTCTGCATTTCTTAGAGCCATCAAGTTCTTAGAAGATCACTGGAAAGAGCTGAGCCACAACATAAGAACTGGCCATGTCAGCGACTGGATCACAGACCCCAGCTGCCGAGCGGCTGTTTTGTTAATCCTCTCGAACCCCGACGTTGGTTTGGCCGATTCGATTGACGCGGAGTGCGGGGAGAAGTCGTGGGAAGGGATAATCAAGAGACTTTGGCCTAAAACCAAGTACATTGAGGTTATTGTTACTGGTTCTATGGCTCAGTATATTTCCACTCTTGAATTCTATAGTGGTGGTCTTCCTTTGGTTTCCACCATGTATGCTTCCTCTGAGTGCTATTTTGGAATCAATATCAATCCTTTGAACAAACCATGTGATGTTTCTTATACCCTTCTTCCGAACATGGGGTTTTTCGAGTTCTTGCCTGTTCAACACCAGAGTTACTGCAATGGAGGCTCTGTTCAAACAGTTGAAGAGTTCGAAACAGTGGATCTCGTCGATGTGAAGCTCGGTCACTATTACGAATTAGTGGTCACAACTTTCACAGGTGACAACCCATCTTTCCCTTTTCCTTTTGGGATCTGACCCACATCATTTATGAATAATCAAAACAACAATAAAGAATTAAAGAAACATGAAACAAAGAACACATTTATTTATGTGGTTCACAAACATCGTGGCAGTTACGTTTATGGACAAATGTCATAAACTGTTTAATTTAGAAGATAAGAATACAAATACTGAATTCAACTGTGCTAGGATTTTAAGAGAGTTTACAGAGTGTAGTTTCAAATAATCCTAATAATACAATGTATGTCTCAAAATATGTAAAAGTCCAATACGAAAGTATAAACTCAATCGCGTATTTTTATTGTGTTTTGAGGGTGTGCCCTGAGCCTCTCAGTTAGCTGACCTAGCCGCAAGATCCCTATTAAGAAATCATTACGCAACATAGTCCAAGACATTTCAGTCATCATTTTTTAAGAATAAGACTATTCAACTAAACCCATGTTCAAAATCATTTTTTGGTGTCTTTTTCTGGATGTATTCAGGGCTATACAGATACAGAGTTGGAGACATCCTAATGGTAACTGGATTCCACAACAACGCCCCTCAATTCCGATTCATTCATCGACGCAACGTCGTGTTGAGTATCGACACCGACAAAACAAACGAAGATGACCTGTTGAAGGCAATAACCAACGCAAAAACATTGCTCGAGCCGCTTGGCTTTTTGCTGACGGAGTACACTAGCTATGCAGACACAGGCTCAATCCCAGGCCATTATGTGCTGTTTTGGGAGCTGAAGATGAGAGGAAGCAGCGAGAATTTAGCCGAGCTAGACTCGGGTTTGATGGAAGAATGCTGTTCGAGCGTAGAACAATCTCTGGATTATGTGTACAGAAGGTGCAGAAACAAGGACAAATCCATTGGAGCATTGGAGATTAGAGTGGTGAAACATGGAGCTTTTGATGCACTTATGGATTTTTGTGTGTCACAAGGCTCATCTGTGAACCAATACAAGACACCAAGATGCATTAAATCAGAAGAGGCTATTAAAATTCTGGATTCCAGATTGGTGGGGAGATTTTACAGCAAGAAAATGCCATTTTGGGAGCCTTTCAGAGTGTAGCAGTGTGTTTAATTATGTGCATTGTTTTAAAAAAGACATGCTTTTTATGAAGAAACTGTTATCTTTTTCATGATTTGTGTTGGAATTATTTGAAGAAAGTTACAGTTTTTTTTTAGCATCATTTGTGTTGAAATCCTTGC

mRNA sequence

TGGAAACAGAATCCCTCCACACAGAAAAAAAAAACAAAAAACTTGCACAATCAAATTGTCAGTTTATGGTTATAGTTATGAGCTGAATTCAAGAAAAAAACAGCTTTGATGCCATGTTGTAAAACTAAACTTTTGTGTTCAAATCCCAAACTCTTTCAGTTCAAACCCCTCTTTTGTGTTCTTGATTTCTTAGATATGTTGCCACAATTTGATCCCAACAACAATGAAGATGGGTTGAAGATTCTTGAGGATCTGACTGAAAATGCAGGGCAAATACAAGAGCAGGTTATTCAGAAGATTCTTGCACAAAACTTAAACACTGAATATCTCAAAAACTTCATCAATGGCGACTCTGCTGACGTGGAACTTTTCAAGAAATCTGTCCCTGTGGTCAACTATGAAGATATCAAGCCTTATGTTGAGAGAATTGCCAATGGGGAGCCTTCCCACATAATTTCAGCTCAACCCATCACCGAGCTGCTCACAAGCTCTGGAACATCTGGAGGACAGCCAAAGATGATGCCTTCTACAGAAGAAGATTTGGACAGAAAGACATTCTTTTACAACCTCCTCATCCCAGTGATGAACAAGTATGTGGATGGATTAGAGGAAGGAAAAGGGATGTACCTATTGTTTATAAAGCCAGAAATCAGCACCCCATCAGGGTTAATGGCAAGGCCAGTTCTCACAAGCTATTACAAAAGCAAAAACTTCACAAACAGACTATTCAATCAGTACAATGTTTACACAAGCCCCAATGAGACAATTTTGTGCTCTGACAGCCGACAGAGCATGTACTGCCAATTGCTCTGTGGGTTAGTCCAAAGAGACCATGTTCTAAGAGTTGGTGCTGTTTTTGCCTCTGCATTTCTTAGAGCCATCAAGTTCTTAGAAGATCACTGGAAAGAGCTGAGCCACAACATAAGAACTGGCCATGTCAGCGACTGGATCACAGACCCCAGCTGCCGAGCGGCTGTTTTGTTAATCCTCTCGAACCCCGACGTTGGTTTGGCCGATTCGATTGACGCGGAGTGCGGGGAGAAGTCGTGGGAAGGGATAATCAAGAGACTTTGGCCTAAAACCAAGTACATTGAGGTTATTGTTACTGGTTCTATGGCTCAGTATATTTCCACTCTTGAATTCTATAGTGGTGGTCTTCCTTTGGTTTCCACCATGTATGCTTCCTCTGAGTGCTATTTTGGAATCAATATCAATCCTTTGAACAAACCATGTGATGTTTCTTATACCCTTCTTCCGAACATGGGGTTTTTCGAGTTCTTGCCTGTTCAACACCAGAGTTACTGCAATGGAGGCTCTGTTCAAACAGTTGAAGAGTTCGAAACAGTGGATCTCGTCGATGTGAAGCTCGGTCACTATTACGAATTAGTGGTCACAACTTTCACAGGGCTATACAGATACAGAGTTGGAGACATCCTAATGGTAACTGGATTCCACAACAACGCCCCTCAATTCCGATTCATTCATCGACGCAACGTCGTGTTGAGTATCGACACCGACAAAACAAACGAAGATGACCTGTTGAAGGCAATAACCAACGCAAAAACATTGCTCGAGCCGCTTGGCTTTTTGCTGACGGAGTACACTAGCTATGCAGACACAGGCTCAATCCCAGGCCATTATGTGCTGTTTTGGGAGCTGAAGATGAGAGGAAGCAGCGAGAATTTAGCCGAGCTAGACTCGGGTTTGATGGAAGAATGCTGTTCGAGCGTAGAACAATCTCTGGATTATGTGTACAGAAGGTGCAGAAACAAGGACAAATCCATTGGAGCATTGGAGATTAGAGTGGTGAAACATGGAGCTTTTGATGCACTTATGGATTTTTGTGTGTCACAAGGCTCATCTGTGAACCAATACAAGACACCAAGATGCATTAAATCAGAAGAGGCTATTAAAATTCTGGATTCCAGATTGGTGGGGAGATTTTACAGCAAGAAAATGCCATTTTGGGAGCCTTTCAGAGTGTAGCAGTGTGTTTAATTATGTGCATTGTTTTAAAAAAGACATGCTTTTTATGAAGAAACTGTTATCTTTTTCATGATTTGTGTTGGAATTATTTGAAGAAAGTTACAGTTTTTTTTTAGCATCATTTGTGTTGAAATCCTTGC

Coding sequence (CDS)

ATGCCATGTTGTAAAACTAAACTTTTGTGTTCAAATCCCAAACTCTTTCAGTTCAAACCCCTCTTTTGTGTTCTTGATTTCTTAGATATGTTGCCACAATTTGATCCCAACAACAATGAAGATGGGTTGAAGATTCTTGAGGATCTGACTGAAAATGCAGGGCAAATACAAGAGCAGGTTATTCAGAAGATTCTTGCACAAAACTTAAACACTGAATATCTCAAAAACTTCATCAATGGCGACTCTGCTGACGTGGAACTTTTCAAGAAATCTGTCCCTGTGGTCAACTATGAAGATATCAAGCCTTATGTTGAGAGAATTGCCAATGGGGAGCCTTCCCACATAATTTCAGCTCAACCCATCACCGAGCTGCTCACAAGCTCTGGAACATCTGGAGGACAGCCAAAGATGATGCCTTCTACAGAAGAAGATTTGGACAGAAAGACATTCTTTTACAACCTCCTCATCCCAGTGATGAACAAGTATGTGGATGGATTAGAGGAAGGAAAAGGGATGTACCTATTGTTTATAAAGCCAGAAATCAGCACCCCATCAGGGTTAATGGCAAGGCCAGTTCTCACAAGCTATTACAAAAGCAAAAACTTCACAAACAGACTATTCAATCAGTACAATGTTTACACAAGCCCCAATGAGACAATTTTGTGCTCTGACAGCCGACAGAGCATGTACTGCCAATTGCTCTGTGGGTTAGTCCAAAGAGACCATGTTCTAAGAGTTGGTGCTGTTTTTGCCTCTGCATTTCTTAGAGCCATCAAGTTCTTAGAAGATCACTGGAAAGAGCTGAGCCACAACATAAGAACTGGCCATGTCAGCGACTGGATCACAGACCCCAGCTGCCGAGCGGCTGTTTTGTTAATCCTCTCGAACCCCGACGTTGGTTTGGCCGATTCGATTGACGCGGAGTGCGGGGAGAAGTCGTGGGAAGGGATAATCAAGAGACTTTGGCCTAAAACCAAGTACATTGAGGTTATTGTTACTGGTTCTATGGCTCAGTATATTTCCACTCTTGAATTCTATAGTGGTGGTCTTCCTTTGGTTTCCACCATGTATGCTTCCTCTGAGTGCTATTTTGGAATCAATATCAATCCTTTGAACAAACCATGTGATGTTTCTTATACCCTTCTTCCGAACATGGGGTTTTTCGAGTTCTTGCCTGTTCAACACCAGAGTTACTGCAATGGAGGCTCTGTTCAAACAGTTGAAGAGTTCGAAACAGTGGATCTCGTCGATGTGAAGCTCGGTCACTATTACGAATTAGTGGTCACAACTTTCACAGGGCTATACAGATACAGAGTTGGAGACATCCTAATGGTAACTGGATTCCACAACAACGCCCCTCAATTCCGATTCATTCATCGACGCAACGTCGTGTTGAGTATCGACACCGACAAAACAAACGAAGATGACCTGTTGAAGGCAATAACCAACGCAAAAACATTGCTCGAGCCGCTTGGCTTTTTGCTGACGGAGTACACTAGCTATGCAGACACAGGCTCAATCCCAGGCCATTATGTGCTGTTTTGGGAGCTGAAGATGAGAGGAAGCAGCGAGAATTTAGCCGAGCTAGACTCGGGTTTGATGGAAGAATGCTGTTCGAGCGTAGAACAATCTCTGGATTATGTGTACAGAAGGTGCAGAAACAAGGACAAATCCATTGGAGCATTGGAGATTAGAGTGGTGAAACATGGAGCTTTTGATGCACTTATGGATTTTTGTGTGTCACAAGGCTCATCTGTGAACCAATACAAGACACCAAGATGCATTAAATCAGAAGAGGCTATTAAAATTCTGGATTCCAGATTGGTGGGGAGATTTTACAGCAAGAAAATGCCATTTTGGGAGCCTTTCAGAGTGTAG

Protein sequence

MPCCKTKLLCSNPKLFQFKPLFCVLDFLDMLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDSADVELFKKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRKTFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFNQYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKELSHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYIEVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFFEFLPVQHQSYCNGGSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILMVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADTGSIPGHYVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEPFRV

Homology

[Close]

BLAST of Sed0020106 vs. NCBI nr
Match: KAG6581972.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018391.1 Indole-3-acetic acid-amido synthetase GH3.17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1054.7 bits (2726), Expect = 3.2e-304
Identity = 516/600 (86.00%), Postives = 550/600 (91.67%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFING-DSADVELF 89
           MLP FDPN+NE GLK+LEDLT+NA QIQEQVI+KIL QN NTEYLK+FING  S DV LF
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 90  KKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRK 149
           K +VPVVNYEDIKPY+ERIANGEPS+IISAQPITELLTSSGTSGG PKMMPST EDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 150 TFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFN 209
           TFFYNLL+PVMNKYVDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNF NR FN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 210 QYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKEL 269
           +YNVYTSP+ETILC+DSRQSMYCQLLCGLVQRD VLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 270 SHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYI 329
           S NIR G+VSDWITDP+CRA+V L+L+ P+  LAD IDA CGEK WEGIIK++WPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 330 EVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFF 389
           EVIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGIN NPL+KP DVSYTLLPNM FF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 390 EFLPVQHQ----SYCNGGSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 449
           EFLPV+      S+CNGGSV   +EFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 450 VTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADT 509
           VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AK LLEPLG LLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 510 GSIPGHYVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEI 569
           GSIPGHYVLFWELK RG  E+L EL+S LMEECCSSVEQSLD VYRRCR+KDKSIG LEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIGPLEI 540

Query: 570 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEPFR 625
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGRF+S+K PFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFWEPFR 600

[Close]

BLAST of Sed0020106 vs. NCBI nr
Match: XP_022980842.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita maxima])

HSP 1 Score: 1053.9 bits (2724), Expect = 5.4e-304
Identity = 516/600 (86.00%), Postives = 550/600 (91.67%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFING-DSADVELF 89
           ML  FDPN+NE GLK+LEDLT+NA QIQEQVI+KIL QN NTEYLK+FING  S DV LF
Sbjct: 1   MLSSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 90  KKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRK 149
           K +VPVVNYEDIKPY+ERIANGEPS+IISAQPITELLTSSGTSGGQPKMMPST EDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120

Query: 150 TFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFN 209
           TFFYNLL+PVMNKYVDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNF NR FN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 210 QYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKEL 269
           +YNVYTSP+ETILC+DSRQSMYCQLLCGLVQRD VLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 270 SHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYI 329
           S NIR G+VSDWITDP+CRA+V L+L+ P+  LAD ID+ CGEK WEGIIK++WPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDSMCGEKLWEGIIKKIWPKTKYI 300

Query: 330 EVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFF 389
           EVIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGIN NPL+KP DVSYTLLPNM FF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 390 EFLPVQHQ----SYCNGGSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 449
           EFLPV+      S+CNGGSV   +EFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 450 VTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADT 509
           VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AK LLEPLG LLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 510 GSIPGHYVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEI 569
           GSIPGHYVLFWELK RG  E+L EL+S LMEECCSSVEQSLD VYRRCR+KDKSIG LEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIGPLEI 540

Query: 570 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEPFR 625
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSR VGRF+S+K PFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRAVGRFFSRKTPFWEPFR 600

[Close]

BLAST of Sed0020106 vs. NCBI nr
Match: XP_023528388.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1052.7 bits (2721), Expect = 1.2e-303
Identity = 515/600 (85.83%), Postives = 550/600 (91.67%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFING-DSADVELF 89
           MLP FDPN++E GLK+LEDLT+NA QIQEQVI+KIL QN NTEYLK+FING  S DV LF
Sbjct: 1   MLPSFDPNDDEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 90  KKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRK 149
           K +VPVVNYEDIKPY+ERIANGEPS+IISAQPITELLTSSGTSGG PKMMPST EDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 150 TFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFN 209
           TFFYNLL+PVMNKYVDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNF NR FN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 210 QYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKEL 269
           +YNVYTSP+ETILC+DSRQSMYCQLLCGLVQRD VLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 270 SHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYI 329
           S NIR G+VSDWITDP+CRA+V L+L+ P+  LAD IDA CGEK WEGIIK++WPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 330 EVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFF 389
           EVIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGIN NPL+KP DVSYTLLPNM FF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 390 EFLPVQHQ----SYCNGGSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 449
           EFLPV+      S+CNGGSV   +EFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 450 VTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADT 509
           VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AK LLEPLG LLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 510 GSIPGHYVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEI 569
           GSIPGHYVLFWELK RG  E+L EL+S LMEECCSSVEQSLD VYRRCR+KDKSIG LEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIGPLEI 540

Query: 570 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEPFR 625
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGRF+S+K PFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFWEPFR 600

[Close]

BLAST of Sed0020106 vs. NCBI nr
Match: XP_022955484.1 (indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita moschata])

HSP 1 Score: 1049.3 bits (2712), Expect = 1.3e-302
Identity = 513/600 (85.50%), Postives = 549/600 (91.50%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFING-DSADVELF 89
           MLP FDPN+NE GLK+LEDLT+NA QIQEQVI+KIL QN NTEYLK+FING  S DV LF
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 90  KKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRK 149
           K +VPVVNYEDIKPY+ERIANGEPS+IISAQPI ELLTSSGTSGG PKMMPST EDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPIIELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 150 TFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFN 209
           TFFYNLL+PVMNKYVDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNF NR FN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 210 QYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKEL 269
           +YNVYTSP+ETILC+DSRQSMYCQLLCGLVQR+ VLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQREDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 270 SHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYI 329
           S NIR G+VSDWITDP+CRA+V L+L+ P+  LAD IDA CGEK WEGIIK++WPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 330 EVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFF 389
           EVIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGIN NPL+KP DVSYTLLPNM FF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 390 EFLPVQHQ----SYCNGGSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 449
           EFLPV+      S+CNGGSV   +EFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 450 VTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADT 509
           VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AK LLEPLG LLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 510 GSIPGHYVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEI 569
           GSIPGHYVLFWELK RG  E+L EL+S LMEECCSSVE+SLD VYRRCR+KDKSIG LEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEKSLDSVYRRCRSKDKSIGPLEI 540

Query: 570 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEPFR 625
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGRF+S+K PFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFWEPFR 600

[Close]

BLAST of Sed0020106 vs. NCBI nr
Match: XP_038900786.1 (indole-3-acetic acid-amido synthetase GH3.17-like [Benincasa hispida])

HSP 1 Score: 1024.6 bits (2648), Expect = 3.5e-295
Identity = 502/609 (82.43%), Postives = 542/609 (89.00%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDSADVELFK 89
           MLP+FDP NNE GLK+LEDLT+NA QIQEQVIQKIL QN NTEYL  F+NG S D++ FK
Sbjct: 1   MLPKFDPTNNESGLKLLEDLTQNAKQIQEQVIQKILTQNFNTEYLNTFLNGHSLDLQTFK 60

Query: 90  KSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRKT 149
            SVPVVNYEDIKPY+ERIANGEPS IIS+QPITELLTSSGTSGGQPKMMPST EDLD KT
Sbjct: 61  NSVPVVNYEDIKPYIERIANGEPSQIISSQPITELLTSSGTSGGQPKMMPSTTEDLDNKT 120

Query: 150 FFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFNQ 209
           FFYNLL+PV+NKYVDGLEEGKGMYLLFIKPE+STPSGLMARPVLTSYYKSKNF NR FN+
Sbjct: 121 FFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNRPFNK 180

Query: 210 YNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKELS 269
           YNVYTSP+ETILCSDS+QSMYCQLLCGLVQRD VLRVGAVFASAFLRAIKFLED+WKELS
Sbjct: 181 YNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYWKELS 240

Query: 270 HNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYIE 329
            NIR G +S WITDP+CR AV  IL+ P+  LAD ID  C EKSWEGIIK++WPKTKYIE
Sbjct: 241 DNIRNGEISKWITDPNCRVAVSSILTKPNAALADLIDGLCCEKSWEGIIKKIWPKTKYIE 300

Query: 330 VIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFFE 389
           VIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGIN NPL+KPCDVSYTLLPNM FFE
Sbjct: 301 VIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPCDVSYTLLPNMAFFE 360

Query: 390 FLPVQHQ----SYCNG-GSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 449
           FLPV+      S+CNG  SVQ ++E +TVDLVDVKLG YYELVVTTFTGLYRYRVGDILM
Sbjct: 361 FLPVEKNDGELSHCNGRSSVQAIDELKTVDLVDVKLGQYYELVVTTFTGLYRYRVGDILM 420

Query: 450 VTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADT 509
           VTGFHNNAPQF+FIHRRNVVLSIDTDKTNEDDLL AIT AK L+EPLG LLTEYTSYADT
Sbjct: 421 VTGFHNNAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLIEPLGVLLTEYTSYADT 480

Query: 510 GSIPGHYVLFWELKMRGSSENLAE---------LDSGLMEECCSSVEQSLDYVYRRCRNK 569
            SIPGHYV+FWELK R  +ENL E         ++ GLMEECCSS+E+SLD VYRRCR+K
Sbjct: 481 ESIPGHYVVFWELKKREGNENLEEENEEEEEEVVEVGLMEECCSSIEESLDSVYRRCRSK 540

Query: 570 DKSIGALEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSK 625
           DKSIGALEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSR+V R++SK
Sbjct: 541 DKSIGALEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSK 600

[Close]

BLAST of Sed0020106 vs. ExPASy Swiss-Prot
Match: Q9FZ87 (Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana OX=3702 GN=GH3.17 PE=1 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 5.3e-254
Identity = 425/605 (70.25%), Postives = 507/605 (83.80%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDS-ADVELF 89
           M+P +DPN+ E GLK+LEDLT NA  IQ+QV+ +IL+QN  T+YL+ F++G++  + + F
Sbjct: 1   MIPSYDPNDTEAGLKLLEDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLDGEADKNQQSF 60

Query: 90  KKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRK 149
           K  VPVVNY+D+KP+++RIA+GE S I+SAQPITELLTSSGTS G+PK+MPST E+L+RK
Sbjct: 61  KNKVPVVNYDDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEELERK 120

Query: 150 TFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFN 209
           TFFY++L+P+MNKYVDGL+EGKGMYLLFIKPEI TPSGLMARPVLTSYYKS++F NR FN
Sbjct: 121 TFFYSMLVPIMNKYVDGLDEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFN 180

Query: 210 QYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKEL 269
           +YNVYTSP++TILC DS+QSMYCQLLCGLVQR HVLRVGAVFASAFLRA+KFLEDH+KEL
Sbjct: 181 KYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKEL 240

Query: 270 SHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYI 329
             +IRTG V+ WITD SCR +VL IL+ P+  LAD I++EC EKSWEGI++R+WPK KY+
Sbjct: 241 CADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAEKSWEGILRRIWPKAKYV 300

Query: 330 EVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFF 389
           EVIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGIN+NPL  P DVSYTLLPNM +F
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYF 360

Query: 390 EFLPVQHQSY---------CNGGSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRV 449
           EFLPV  +S+                 ++E   V+LV+V++G YYE+V+TTFTGLYRYRV
Sbjct: 361 EFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRV 420

Query: 450 GDILMVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKT--LLEPLGFLLTE 509
           GDIL VTGFHN APQFRF+ RRNVVLSIDTDKT+E+DLL A+T AK   L  P   LLTE
Sbjct: 421 GDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTE 480

Query: 510 YTSYADTGSIPGHYVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDK 569
           YTSYADT SIPGHYVLFWELK R S++   +LD   ME+CCS VE  LDYVYRRCRN+DK
Sbjct: 481 YTSYADTSSIPGHYVLFWELKPRHSNDP-PKLDDKTMEDCCSEVEDCLDYVYRRCRNRDK 540

Query: 570 SIGALEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKM 623
           SIG LEIRVV  G FD+LMDFCVSQGSS+NQYKTPRC+KS  A++ILDSR++GRF+SK++
Sbjct: 541 SIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRV 600

[Close]

BLAST of Sed0020106 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 5.8e-200
Identity = 345/589 (58.57%), Postives = 440/589 (74.70%), Query Frame = 0


Query: 39  NEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDSADVELFKKSVPVVNYE 98
           N++ L+ +ED+T NA  +Q +V+++IL++N + EYLK        D E FK  +PVV YE
Sbjct: 21  NKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYE 80

Query: 99  DIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRKTFFYNLLIPV 158
           DI+P + RIANG+ S ++ + PI+E LTSSGTSGG+ K+MP+ EE+LDR++  Y+LL+PV
Sbjct: 81  DIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPV 140

Query: 159 MNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFNQYNVYTSPNE 218
           M+++V GL++GKGMY LFIK E  TP GL ARPVLTSYYKS +F NR ++ Y  YTSPN+
Sbjct: 141 MDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQ 200

Query: 219 TILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKELSHNIRTGHVS 278
           TILCSDS QSMY Q+LCGL Q   VLRVGAVFAS F+RAIKFLE HW EL+ +IRTG +S
Sbjct: 201 TILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLS 260

Query: 279 DWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYIEVIVTGSMAQ 338
             ITD S R AV  IL  PD  LAD +++EC + SW+GII RLWP TKY++VIVTG+M+Q
Sbjct: 261 SEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQ 320

Query: 339 YISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFFEFLPVQHQSY 398
           YI TL++YS GLPLV TMYASSECYFG+N+ PL KP +VSYTL+PNM +FEFLPV   S 
Sbjct: 321 YIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSG 380

Query: 399 CNGG-----SVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILMVTGFHNNAP 458
                    ++   E+ E VDLVDVKLG  YELVVTT+ GLYRYRVGD+L V GF NNAP
Sbjct: 381 VTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAP 440

Query: 459 QFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADTGSIPGHYVL 518
           QF FI R+NVVLSID+DKT+E +L  A+ NA T L P    L+EYTSYADT SIPGHYVL
Sbjct: 441 QFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVL 500

Query: 519 FWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEIRVVKHGAFD 578
           FWEL + G++     +   + E+CC ++E+SL+ VYR+ R  DKSIG LEI++V+ G FD
Sbjct: 501 FWELCLNGNT----PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFD 560

Query: 579 ALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEP 623
            LMD+ +S G+S+NQYKTPRC+K    I++L+SR+V  ++S K P W P
Sbjct: 561 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

[Close]

BLAST of Sed0020106 vs. ExPASy Swiss-Prot
Match: P0C0M3 (Probable indole-3-acetic acid-amido synthetase GH3.11 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.11 PE=2 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 7.6e-200
Identity = 341/577 (59.10%), Postives = 438/577 (75.91%), Query Frame = 0


Query: 43  LKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDSADVELFKKSVPVVNYEDIKP 102
           L+ LE LT NA + QE ++ KIL +N  TEYL  F+NG S ++  FK+ VPVV Y+ + P
Sbjct: 14  LEELEMLTVNAKEAQELILTKILERNQATEYLSKFMNG-STNISAFKRHVPVVTYDKVHP 73

Query: 103 YVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRKTFFYNLLIPVMNKY 162
           Y+ RIA GE S I+  + I ELL SSGTS G+P++MPS  +DLDR+T+ Y+L++P+MNKY
Sbjct: 74  YILRIATGEESSILCGEYILELLRSSGTSRGEPRLMPSILKDLDRRTYLYSLIMPIMNKY 133

Query: 163 VDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFNQYNVYTSPNETILC 222
           + GL EGK MYLLF+K E  T SG+  R VLTSYYKS +F +R  + YN YTSP+E ILC
Sbjct: 134 ISGLGEGKAMYLLFVKAETLTDSGIPVRSVLTSYYKSPHFLHRKHDLYNNYTSPDEVILC 193

Query: 223 SDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKELSHNIRTGHVSDWIT 282
            DS+QSMYCQLLCGLV+R HVLR+GAVFASAFLR+I FLE HW++L ++IR G ++  IT
Sbjct: 194 PDSQQSMYCQLLCGLVERQHVLRIGAVFASAFLRSISFLEQHWRDLVNDIRIGQLNSSIT 253

Query: 283 DPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYIEVIVTGSMAQYIST 342
            P+CR A+L  L+ P+  LAD ++A C   SW+GI+ RLWP  KYIE ++TG+MAQYI  
Sbjct: 254 SPACRLAMLNFLALPNPELADQVEAICSCGSWKGILGRLWPNVKYIEAVLTGTMAQYIPM 313

Query: 343 LEFYSGG-LPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFFEFLPVQHQSYCNG 402
           LEFY GG +P V TMYASSE YFG+N++PL  P DVSYT+LPNM +FEF+P++       
Sbjct: 314 LEFYGGGAIPFVCTMYASSESYFGVNLSPLCSPADVSYTILPNMAYFEFIPLEDGLRLTD 373

Query: 403 GSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILMVTGFHNNAPQFRFIHRR 462
              + +E  + V LVDVK+G YYELVVTTF+GLYRYRVGD+L VTGF+N APQF+FI RR
Sbjct: 374 HE-EVIENDKLVSLVDVKVGCYYELVVTTFSGLYRYRVGDVLQVTGFYNRAPQFKFICRR 433

Query: 463 NVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADTGSIPGHYVLFWELKMRG 522
           NV+LSID+DKTNE+DL  ++T AK +LE   +LL EYTSY D  ++PGHYVLFWE+K   
Sbjct: 434 NVILSIDSDKTNEEDLHNSVTTAKKILENQNYLLLEYTSYTDISTVPGHYVLFWEIKST- 493

Query: 523 SSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEIRVVKHGAFDALMDFCVS 582
             E  A LD+ L+E CC++VE+SLDYVYRRCR  D+SIG LEIR+V+ GAFDALMD  VS
Sbjct: 494 HDERPAPLDAQLLESCCAAVEESLDYVYRRCRAHDRSIGPLEIRLVEAGAFDALMDLLVS 553

Query: 583 QGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMP 619
            GSS+NQYKTPRCI+S  A+K+L+S+++  F+S + P
Sbjct: 554 HGSSINQYKTPRCIESSLALKLLNSKVIACFFSPQDP 587

[Close]

BLAST of Sed0020106 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 1.1e-198
Identity = 345/589 (58.57%), Postives = 436/589 (74.02%), Query Frame = 0


Query: 39  NEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDSADVELFKKSVPVVNYE 98
           N+  L+++E+LT NA Q+Q QV+++IL +N + EYL+        D E FK  +PV+ YE
Sbjct: 21  NKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVITYE 80

Query: 99  DIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRKTFFYNLLIPV 158
           DI+P + RIANG+ S I+S++PI+E LTSSGTSGG+ K+MP+ EE+LDR++  Y+LL+PV
Sbjct: 81  DIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPV 140

Query: 159 MNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFNQYNVYTSPNE 218
           M+++V GLE GKGMY LFIK E  TP GL ARPVLTSYYKS +F  R ++ Y  YTSPNE
Sbjct: 141 MSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNE 200

Query: 219 TILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKELSHNIRTGHVS 278
           TILCSDS QSMY Q+LCGL Q   VLRVGAVFAS F+RAIKFLE HW EL  +IRTG +S
Sbjct: 201 TILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLS 260

Query: 279 DWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYIEVIVTGSMAQ 338
             ITDPS R AV  IL  P   LAD ++ EC + SW+GII RLWP TKY++VIVTG+M+Q
Sbjct: 261 SLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQ 320

Query: 339 YISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFFEFLPVQHQSY 398
           YI TL++YS GLPLV TMYASSECYFG+N+ PL KP +VSYTL+P+M +FEFLPV   + 
Sbjct: 321 YIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNG 380

Query: 399 CNGG-----SVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILMVTGFHNNAP 458
                    ++   E+ E VDLVDVKLG  YELVVTT+ GL RYRVGD+L VTGF N AP
Sbjct: 381 VTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKAP 440

Query: 459 QFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADTGSIPGHYVL 518
           QF FI R+NVVLSID+DKT+E +L  A+ NA T L P    L+EYTSYADT SIPGHYVL
Sbjct: 441 QFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVL 500

Query: 519 FWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEIRVVKHGAFD 578
           FWEL + G++     +   + E+CC +VE+S + VYR+ R  DKSIG LEI++V+ G FD
Sbjct: 501 FWELCLDGNT----PIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 560

Query: 579 ALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEP 623
            LMD+ +S G+S+NQYKTPRC+K    I++L+SR+V  ++S K P W P
Sbjct: 561 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

[Close]

BLAST of Sed0020106 vs. ExPASy Swiss-Prot
Match: O82243 (Putative indole-3-acetic acid-amido synthetase GH3.9 OS=Arabidopsis thaliana OX=3702 GN=GH3.9 PE=2 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 1.4e-193
Identity = 338/593 (57.00%), Postives = 435/593 (73.36%), Query Frame = 0


Query: 38  NNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDSADVELFKKSVPVVNY 97
           +++  LK LE +T  A ++Q+ +++ IL +N +TEYL  ++NG S DV  FK++VP++ Y
Sbjct: 7   DHDSVLKELERITSKAAEVQDNILRGILERNKDTEYLSKYMNG-SKDVLEFKRAVPIIIY 66

Query: 98  EDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRKTFFYNLLIP 157
           +DI PY++RIANGE S +I+   ITE+L SSGTS G+PK+MP+  EDLDR+TF YNL+IP
Sbjct: 67  KDIYPYIQRIANGEDSSLITGHSITEILCSSGTSAGEPKLMPTIPEDLDRRTFLYNLIIP 126

Query: 158 VMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFNQYNVYTSPN 217
           ++NKY+ GL++GK MYL F+K E STP GL  R VLTSYYKSK+F  R ++ +N  TSP 
Sbjct: 127 IVNKYITGLDKGKAMYLNFVKAETSTPCGLPIRAVLTSYYKSKHFQCRPYDPFNDLTSPI 186

Query: 218 ETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKELSHNIRTGHV 277
           +TILC DS QSMYCQLL GL+ R  V+R+GAVFASAFLRAI +LE  W +L  +IRTG +
Sbjct: 187 QTILCEDSNQSMYCQLLAGLIHRHKVMRLGAVFASAFLRAISYLEKKWSQLCEDIRTGSL 246

Query: 278 SDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYIEVIVTGSMA 337
           +  ITDP C+ A+  +L +P+  LA  I+  CG  SW+GI+ +LWPK K+IE +VTGSMA
Sbjct: 247 NPMITDPGCQMAMSCLLMSPNPELASEIEEICGRSSWKGILCQLWPKAKFIEAVVTGSMA 306

Query: 338 QYISTLEFYS-GGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFFEFLPVQHQ 397
           QYI  LEF+S G +PLV  MYASSE YFG+N+ PL+KP DV +TLLPNM +FEF+P+   
Sbjct: 307 QYIPALEFFSQGKIPLVCPMYASSETYFGVNVEPLSKPSDVVFTLLPNMCYFEFIPLGK- 366

Query: 398 SYCNGGSVQTVEEFE------TVDLVDVKLGHYYELVVTTFTGLYRYRVGDILMVTGFHN 457
              NG     +++ E       VDLV+VKLG YYELVVTTF GLYRYR+GD+L V GF+N
Sbjct: 367 ---NGTLSFDLDDDEQVPCDKVVDLVNVKLGRYYELVVTTFAGLYRYRIGDVLQVAGFYN 426

Query: 458 NAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADTGSIPGH 517
            APQFRFI RRNVVLSID DKTNE+DL ++IT AK  L    F L EYTSYADT S+PGH
Sbjct: 427 GAPQFRFICRRNVVLSIDLDKTNEEDLHRSITLAKKKLGSNAF-LAEYTSYADTSSVPGH 486

Query: 518 YVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEIRVVKHG 577
           YVLFWE++          L+  LMEECC +VE+ LDY+YR+CR K++SIGALEIRVVK G
Sbjct: 487 YVLFWEIQ--------GHLEPKLMEECCVAVEEELDYIYRQCRTKERSIGALEIRVVKPG 546

Query: 578 AFDALMDFCVSQGSSVNQYKTPRCIKSEEA-IKILDSRLVGRFYSKKMPFWEP 623
            F+ LMD  +SQG S NQYKTPRC+KS  A  K+L+  ++  F+S + P W P
Sbjct: 547 TFEKLMDLIISQGGSFNQYKTPRCVKSNSATFKLLNGHVMASFFSPRDPTWVP 585

[Close]

BLAST of Sed0020106 vs. ExPASy TrEMBL
Match: A0A6J1ISD7 (indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita maxima OX=3661 GN=LOC111480114 PE=3 SV=1)

HSP 1 Score: 1053.9 bits (2724), Expect = 2.6e-304
Identity = 516/600 (86.00%), Postives = 550/600 (91.67%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFING-DSADVELF 89
           ML  FDPN+NE GLK+LEDLT+NA QIQEQVI+KIL QN NTEYLK+FING  S DV LF
Sbjct: 1   MLSSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 90  KKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRK 149
           K +VPVVNYEDIKPY+ERIANGEPS+IISAQPITELLTSSGTSGGQPKMMPST EDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAEDLDRK 120

Query: 150 TFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFN 209
           TFFYNLL+PVMNKYVDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNF NR FN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 210 QYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKEL 269
           +YNVYTSP+ETILC+DSRQSMYCQLLCGLVQRD VLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 270 SHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYI 329
           S NIR G+VSDWITDP+CRA+V L+L+ P+  LAD ID+ CGEK WEGIIK++WPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDSMCGEKLWEGIIKKIWPKTKYI 300

Query: 330 EVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFF 389
           EVIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGIN NPL+KP DVSYTLLPNM FF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 390 EFLPVQHQ----SYCNGGSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 449
           EFLPV+      S+CNGGSV   +EFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 450 VTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADT 509
           VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AK LLEPLG LLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 510 GSIPGHYVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEI 569
           GSIPGHYVLFWELK RG  E+L EL+S LMEECCSSVEQSLD VYRRCR+KDKSIG LEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEQSLDSVYRRCRSKDKSIGPLEI 540

Query: 570 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEPFR 625
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSR VGRF+S+K PFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRAVGRFFSRKTPFWEPFR 600

[Close]

BLAST of Sed0020106 vs. ExPASy TrEMBL
Match: A0A6J1GWE7 (indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita moschata OX=3662 GN=LOC111457496 PE=3 SV=1)

HSP 1 Score: 1049.3 bits (2712), Expect = 6.5e-303
Identity = 513/600 (85.50%), Postives = 549/600 (91.50%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFING-DSADVELF 89
           MLP FDPN+NE GLK+LEDLT+NA QIQEQVI+KIL QN NTEYLK+FING  S DV LF
Sbjct: 1   MLPSFDPNDNEGGLKLLEDLTQNARQIQEQVIEKILTQNANTEYLKSFINGCHSPDVHLF 60

Query: 90  KKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRK 149
           K +VPVVNYEDIKPY+ERIANGEPS+IISAQPI ELLTSSGTSGG PKMMPST EDLDRK
Sbjct: 61  KNTVPVVNYEDIKPYIERIANGEPSNIISAQPIIELLTSSGTSGGHPKMMPSTAEDLDRK 120

Query: 150 TFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFN 209
           TFFYNLL+PVMNKYVDGLE+GKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNF NR FN
Sbjct: 121 TFFYNLLVPVMNKYVDGLEQGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRNRPFN 180

Query: 210 QYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKEL 269
           +YNVYTSP+ETILC+DSRQSMYCQLLCGLVQR+ VLRVGAVFASAFLRAIKFLEDHWKE+
Sbjct: 181 RYNVYTSPDETILCADSRQSMYCQLLCGLVQREDVLRVGAVFASAFLRAIKFLEDHWKEM 240

Query: 270 SHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYI 329
           S NIR G+VSDWITDP+CRA+V L+L+ P+  LAD IDA CGEK WEGIIK++WPKTKYI
Sbjct: 241 SDNIRHGYVSDWITDPNCRASVSLLLTKPNAALADLIDAMCGEKLWEGIIKKIWPKTKYI 300

Query: 330 EVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFF 389
           EVIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGIN NPL+KP DVSYTLLPNM FF
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFNPLSKPSDVSYTLLPNMAFF 360

Query: 390 EFLPVQHQ----SYCNGGSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 449
           EFLPV+      S+CNGGSV   +EFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM
Sbjct: 361 EFLPVEKNNGELSHCNGGSVPPADEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILM 420

Query: 450 VTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADT 509
           VTGFHN+APQFRF+HRRNVVLSIDTDKTNEDDLL AIT AK LLEPLG LLTEYTSYADT
Sbjct: 421 VTGFHNSAPQFRFVHRRNVVLSIDTDKTNEDDLLNAITKAKLLLEPLGVLLTEYTSYADT 480

Query: 510 GSIPGHYVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEI 569
           GSIPGHYVLFWELK RG  E+L EL+S LMEECCSSVE+SLD VYRRCR+KDKSIG LEI
Sbjct: 481 GSIPGHYVLFWELKNRGGCESLGELESSLMEECCSSVEKSLDSVYRRCRSKDKSIGPLEI 540

Query: 570 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEPFR 625
           RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAI+ILDSR VGRF+S+K PFWEPFR
Sbjct: 541 RVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIRILDSRAVGRFFSRKTPFWEPFR 600

[Close]

BLAST of Sed0020106 vs. ExPASy TrEMBL
Match: A0A6J1DHN6 (indole-3-acetic acid-amido synthetase GH3.17-like OS=Momordica charantia OX=3673 GN=LOC111020995 PE=3 SV=1)

HSP 1 Score: 1009.6 bits (2609), Expect = 5.7e-291
Identity = 503/617 (81.52%), Postives = 549/617 (88.98%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFING-----DSAD 89
           MLP FDPN+NE GLK+LEDLT+NA QIQEQVIQKIL+QN NTEYLKNFIN       S D
Sbjct: 1   MLPNFDPNDNESGLKLLEDLTQNARQIQEQVIQKILSQNANTEYLKNFINNGCCSHSSPD 60

Query: 90  VELFKKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEED 149
           ++LFK SVPVVNYEDIKPY+ERIANGEPS+IISAQPITELLTSSGTSGGQPKMMPST ED
Sbjct: 61  LKLFKNSVPVVNYEDIKPYIERIANGEPSNIISAQPITELLTSSGTSGGQPKMMPSTAED 120

Query: 150 LDRKTFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTN 209
           LDRKTFFYNLL+P+MNK+V GLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNF N
Sbjct: 121 LDRKTFFYNLLVPIMNKHVGGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFRN 180

Query: 210 RLFNQYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDH 269
           R FN+YNVYTSP+ETILCSDS+QSMYCQLLCGL+QRD VLRVGAVFASAFLRAIKFLED+
Sbjct: 181 RPFNRYNVYTSPDETILCSDSKQSMYCQLLCGLIQRDEVLRVGAVFASAFLRAIKFLEDY 240

Query: 270 WKELSHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPK 329
           WKE+SHNIR+G VS+WITDPSCR++V LIL+ P+  LAD IDA CGEKSWEGIIK++W K
Sbjct: 241 WKEMSHNIRSGCVSEWITDPSCRSSVSLILTKPNEELADLIDAMCGEKSWEGIIKKIWAK 300

Query: 330 TKYIEVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPN 389
           TKYIEVIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGIN +PL+KP DVSYTLLPN
Sbjct: 301 TKYIEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINFSPLSKPEDVSYTLLPN 360

Query: 390 MGFFEFLPVQHQS---------YCNGGSVQ----TVEEFETVDLVDVKLGHYYELVVTTF 449
           M FFEFLPV+            + NGG+V+     + + ETVDLVDV+LG YYELVVTTF
Sbjct: 361 MAFFEFLPVERNDGGELARSGPHRNGGAVRDDGDEMGKLETVDLVDVELGRYYELVVTTF 420

Query: 450 TGLYRYRVGDILMVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPL 509
           TGLYRYRVGDIL VTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAK L+EPL
Sbjct: 421 TGLYRYRVGDILKVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKLLVEPL 480

Query: 510 GFLLTEYTSYADTGSIPGHYVLFWELKMR-GSSENLA--ELDSGLMEECCSSVEQSLDYV 569
           G LLTEY+SYADT SIPGHYVLFWELK R G  EN A   +D G+MEECCSS+E+SLD V
Sbjct: 481 GILLTEYSSYADTSSIPGHYVLFWELKKRGGGGENAAATAVDLGVMEECCSSIEESLDSV 540

Query: 570 YRRCRNKDKSIGALEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRL 626
           YRRCR+KDKSIG LEIRVVK GAFDALMD+CVSQGSSVNQYKTPRCIKSEEAI+ILDSR+
Sbjct: 541 YRRCRSKDKSIGPLEIRVVKGGAFDALMDYCVSQGSSVNQYKTPRCIKSEEAIRILDSRV 600

[Close]

BLAST of Sed0020106 vs. ExPASy TrEMBL
Match: A0A5A7TMG2 (Indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002300 PE=3 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.2e-288
Identity = 498/607 (82.04%), Postives = 540/607 (88.96%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFIN----GDSADV 89
           MLP FDPNNNE GLK+LEDLT NA QIQEQVI+KIL QN NTEYLK+F++      S D+
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIRKILTQNSNTEYLKSFLHNHHYSHSLDL 60

Query: 90  ELFKKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDL 149
           + FK SVPVVNYEDIKPY+ERIANGEPSHIIS+QPITELLTSSGTSGGQPKMMPST EDL
Sbjct: 61  QTFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120

Query: 150 DRKTFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNR 209
           DRKTFFYNLL+PV+NKYVDGLEEGKGMYLLFIKPE+STPSGLMARPVLTSYYKSKNF NR
Sbjct: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNR 180

Query: 210 LFNQYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHW 269
            FN+YNVYTSP+ETILCSDS+QSMYCQLLCGLVQRD VLRVGAVFASAFLRAIKFLED+W
Sbjct: 181 PFNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYW 240

Query: 270 KELSHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKT 329
           KELS NIR G +S WI+DP+CRA+V LIL+  +  LAD ID  CGEKSWEGIIK+LWPKT
Sbjct: 241 KELSDNIRKGELSQWISDPNCRASVSLILTKSNPVLADLIDGLCGEKSWEGIIKKLWPKT 300

Query: 330 KYIEVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNM 389
           KYIEVIVTGSMAQYI TLEFYSGGLPL+STMYASSECYFGIN NPL+KP DVSYTLLPNM
Sbjct: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNM 360

Query: 390 GFFEFLPVQHQ----SYCNGGSVQTV-EEFETVDLVDVKLGHYYELVVTTFTGLYRYRVG 449
            FFEFLPV+      ++CNG +  +V +EF+TVDLVDVKLG YYELVVTTFTGLYRYRVG
Sbjct: 361 AFFEFLPVEKNDGELTHCNGTTNTSVQDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG 420

Query: 450 DILMVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTS 509
           DIL VTGFHN APQF+FIHRRNVVLSIDTDKTNEDDLL AIT AK L+EPLG LLTEYTS
Sbjct: 421 DILKVTGFHNKAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS 480

Query: 510 YADTGSIPGHYVLFWELKMRGSS--ENLAEL-DSGLMEECCSSVEQSLDYVYRRCRNKDK 569
           YADT SIPGHYVLFWE+K R  S  EN  EL +  LMEECCSS+E+SLD VYRRCR+KDK
Sbjct: 481 YADTESIPGHYVLFWEMKRRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCRSKDK 540

Query: 570 SIGALEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKM 625
           SIG LEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSR+V R++SKK 
Sbjct: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSKKT 600

[Close]

BLAST of Sed0020106 vs. ExPASy TrEMBL
Match: A0A1S3BYQ7 (indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo OX=3656 GN=LOC103494645 PE=3 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.2e-288
Identity = 498/607 (82.04%), Postives = 540/607 (88.96%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFIN----GDSADV 89
           MLP FDPNNNE GLK+LEDLT NA QIQEQVI+KIL QN NTEYLK+F++      S D+
Sbjct: 1   MLPNFDPNNNESGLKLLEDLTTNAKQIQEQVIRKILTQNSNTEYLKSFLHNHHYSHSLDL 60

Query: 90  ELFKKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDL 149
           + FK SVPVVNYEDIKPY+ERIANGEPSHIIS+QPITELLTSSGTSGGQPKMMPST EDL
Sbjct: 61  QTFKHSVPVVNYEDIKPYIERIANGEPSHIISSQPITELLTSSGTSGGQPKMMPSTAEDL 120

Query: 150 DRKTFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNR 209
           DRKTFFYNLL+PV+NKYVDGLEEGKGMYLLFIKPE+STPSGLMARPVLTSYYKSKNF NR
Sbjct: 121 DRKTFFYNLLVPVLNKYVDGLEEGKGMYLLFIKPEMSTPSGLMARPVLTSYYKSKNFRNR 180

Query: 210 LFNQYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHW 269
            FN+YNVYTSP+ETILCSDS+QSMYCQLLCGLVQRD VLRVGAVFASAFLRAIKFLED+W
Sbjct: 181 PFNKYNVYTSPDETILCSDSKQSMYCQLLCGLVQRDEVLRVGAVFASAFLRAIKFLEDYW 240

Query: 270 KELSHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKT 329
           KELS NIR G +S WI+DP+CRA+V LIL+  +  LAD ID  CGEKSWEGIIK+LWPKT
Sbjct: 241 KELSDNIRKGELSQWISDPNCRASVSLILTKSNPVLADLIDGLCGEKSWEGIIKKLWPKT 300

Query: 330 KYIEVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNM 389
           KYIEVIVTGSMAQYI TLEFYSGGLPL+STMYASSECYFGIN NPL+KP DVSYTLLPNM
Sbjct: 301 KYIEVIVTGSMAQYIPTLEFYSGGLPLISTMYASSECYFGINFNPLSKPSDVSYTLLPNM 360

Query: 390 GFFEFLPVQHQ----SYCNGGSVQTV-EEFETVDLVDVKLGHYYELVVTTFTGLYRYRVG 449
            FFEFLPV+      ++CNG +  +V +EF+TVDLVDVKLG YYELVVTTFTGLYRYRVG
Sbjct: 361 AFFEFLPVEKNDGELTHCNGTTNTSVQDEFKTVDLVDVKLGQYYELVVTTFTGLYRYRVG 420

Query: 450 DILMVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTS 509
           DIL VTGFHN APQF+FIHRRNVVLSIDTDKTNEDDLL AIT AK L+EPLG LLTEYTS
Sbjct: 421 DILKVTGFHNKAPQFKFIHRRNVVLSIDTDKTNEDDLLNAITKAKLLVEPLGVLLTEYTS 480

Query: 510 YADTGSIPGHYVLFWELKMRGSS--ENLAEL-DSGLMEECCSSVEQSLDYVYRRCRNKDK 569
           YADT SIPGHYVLFWE+K R  S  EN  EL +  LMEECCSS+E+SLD VYRRCR+KDK
Sbjct: 481 YADTESIPGHYVLFWEMKRRSGSNYENFEELVEVDLMEECCSSIEESLDSVYRRCRSKDK 540

Query: 570 SIGALEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKM 625
           SIG LEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSR+V R++SKK 
Sbjct: 541 SIGPLEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRVVARYFSKKT 600

[Close]

BLAST of Sed0020106 vs. TAIR 10
Match: AT1G28130.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 878.2 bits (2268), Expect = 3.8e-255
Identity = 425/605 (70.25%), Postives = 507/605 (83.80%), Query Frame = 0


Query: 30  MLPQFDPNNNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDS-ADVELF 89
           M+P +DPN+ E GLK+LEDLT NA  IQ+QV+ +IL+QN  T+YL+ F++G++  + + F
Sbjct: 1   MIPSYDPNDTEAGLKLLEDLTTNAEAIQQQVLHQILSQNSGTQYLRAFLDGEADKNQQSF 60

Query: 90  KKSVPVVNYEDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRK 149
           K  VPVVNY+D+KP+++RIA+GE S I+SAQPITELLTSSGTS G+PK+MPST E+L+RK
Sbjct: 61  KNKVPVVNYDDVKPFIQRIADGESSDIVSAQPITELLTSSGTSAGKPKLMPSTAEELERK 120

Query: 150 TFFYNLLIPVMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFN 209
           TFFY++L+P+MNKYVDGL+EGKGMYLLFIKPEI TPSGLMARPVLTSYYKS++F NR FN
Sbjct: 121 TFFYSMLVPIMNKYVDGLDEGKGMYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFN 180

Query: 210 QYNVYTSPNETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKEL 269
           +YNVYTSP++TILC DS+QSMYCQLLCGLVQR HVLRVGAVFASAFLRA+KFLEDH+KEL
Sbjct: 181 KYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKEL 240

Query: 270 SHNIRTGHVSDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYI 329
             +IRTG V+ WITD SCR +VL IL+ P+  LAD I++EC EKSWEGI++R+WPK KY+
Sbjct: 241 CADIRTGTVTSWITDSSCRDSVLSILNGPNQELADEIESECAEKSWEGILRRIWPKAKYV 300

Query: 330 EVIVTGSMAQYISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFF 389
           EVIVTGSMAQYI TLEFYSGGLPLVSTMYASSECYFGIN+NPL  P DVSYTLLPNM +F
Sbjct: 301 EVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYF 360

Query: 390 EFLPVQHQSY---------CNGGSVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRV 449
           EFLPV  +S+                 ++E   V+LV+V++G YYE+V+TTFTGLYRYRV
Sbjct: 361 EFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRV 420

Query: 450 GDILMVTGFHNNAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKT--LLEPLGFLLTE 509
           GDIL VTGFHN APQFRF+ RRNVVLSIDTDKT+E+DLL A+T AK   L  P   LLTE
Sbjct: 421 GDILKVTGFHNKAPQFRFVQRRNVVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTE 480

Query: 510 YTSYADTGSIPGHYVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDK 569
           YTSYADT SIPGHYVLFWELK R S++   +LD   ME+CCS VE  LDYVYRRCRN+DK
Sbjct: 481 YTSYADTSSIPGHYVLFWELKPRHSNDP-PKLDDKTMEDCCSEVEDCLDYVYRRCRNRDK 540

Query: 570 SIGALEIRVVKHGAFDALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKM 623
           SIG LEIRVV  G FD+LMDFCVSQGSS+NQYKTPRC+KS  A++ILDSR++GRF+SK++
Sbjct: 541 SIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRV 600

[Close]

BLAST of Sed0020106 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 698.7 bits (1802), Expect = 4.1e-201
Identity = 345/589 (58.57%), Postives = 440/589 (74.70%), Query Frame = 0


Query: 39  NEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDSADVELFKKSVPVVNYE 98
           N++ L+ +ED+T NA  +Q +V+++IL++N + EYLK        D E FK  +PVV YE
Sbjct: 21  NKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVTYE 80

Query: 99  DIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRKTFFYNLLIPV 158
           DI+P + RIANG+ S ++ + PI+E LTSSGTSGG+ K+MP+ EE+LDR++  Y+LL+PV
Sbjct: 81  DIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPV 140

Query: 159 MNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFNQYNVYTSPNE 218
           M+++V GL++GKGMY LFIK E  TP GL ARPVLTSYYKS +F NR ++ Y  YTSPN+
Sbjct: 141 MDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQ 200

Query: 219 TILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKELSHNIRTGHVS 278
           TILCSDS QSMY Q+LCGL Q   VLRVGAVFAS F+RAIKFLE HW EL+ +IRTG +S
Sbjct: 201 TILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGTLS 260

Query: 279 DWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYIEVIVTGSMAQ 338
             ITD S R AV  IL  PD  LAD +++EC + SW+GII RLWP TKY++VIVTG+M+Q
Sbjct: 261 SEITDSSVREAVGEIL-KPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQ 320

Query: 339 YISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFFEFLPVQHQSY 398
           YI TL++YS GLPLV TMYASSECYFG+N+ PL KP +VSYTL+PNM +FEFLPV   S 
Sbjct: 321 YIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSG 380

Query: 399 CNGG-----SVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILMVTGFHNNAP 458
                    ++   E+ E VDLVDVKLG  YELVVTT+ GLYRYRVGD+L V GF NNAP
Sbjct: 381 VTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNNAP 440

Query: 459 QFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADTGSIPGHYVL 518
           QF FI R+NVVLSID+DKT+E +L  A+ NA T L P    L+EYTSYADT SIPGHYVL
Sbjct: 441 QFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVL 500

Query: 519 FWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEIRVVKHGAFD 578
           FWEL + G++     +   + E+CC ++E+SL+ VYR+ R  DKSIG LEI++V+ G FD
Sbjct: 501 FWELCLNGNT----PIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFD 560

Query: 579 ALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEP 623
            LMD+ +S G+S+NQYKTPRC+K    I++L+SR+V  ++S K P W P
Sbjct: 561 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSP 604

[Close]

BLAST of Sed0020106 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 694.5 bits (1791), Expect = 7.8e-200
Identity = 345/589 (58.57%), Postives = 436/589 (74.02%), Query Frame = 0


Query: 39  NEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDSADVELFKKSVPVVNYE 98
           N+  L+++E+LT NA Q+Q QV+++IL +N + EYL+        D E FK  +PV+ YE
Sbjct: 21  NKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPVITYE 80

Query: 99  DIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRKTFFYNLLIPV 158
           DI+P + RIANG+ S I+S++PI+E LTSSGTSGG+ K+MP+ EE+LDR++  Y+LL+PV
Sbjct: 81  DIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLMPV 140

Query: 159 MNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFNQYNVYTSPNE 218
           M+++V GLE GKGMY LFIK E  TP GL ARPVLTSYYKS +F  R ++ Y  YTSPNE
Sbjct: 141 MSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNE 200

Query: 219 TILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKELSHNIRTGHVS 278
           TILCSDS QSMY Q+LCGL Q   VLRVGAVFAS F+RAIKFLE HW EL  +IRTG +S
Sbjct: 201 TILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLS 260

Query: 279 DWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYIEVIVTGSMAQ 338
             ITDPS R AV  IL  P   LAD ++ EC + SW+GII RLWP TKY++VIVTG+M+Q
Sbjct: 261 SLITDPSVREAVAKIL-KPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQ 320

Query: 339 YISTLEFYSGGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFFEFLPVQHQSY 398
           YI TL++YS GLPLV TMYASSECYFG+N+ PL KP +VSYTL+P+M +FEFLPV   + 
Sbjct: 321 YIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNG 380

Query: 399 CNGG-----SVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILMVTGFHNNAP 458
                    ++   E+ E VDLVDVKLG  YELVVTT+ GL RYRVGD+L VTGF N AP
Sbjct: 381 VTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFKNKAP 440

Query: 459 QFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADTGSIPGHYVL 518
           QF FI R+NVVLSID+DKT+E +L  A+ NA T L P    L+EYTSYADT SIPGHYVL
Sbjct: 441 QFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVL 500

Query: 519 FWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEIRVVKHGAFD 578
           FWEL + G++     +   + E+CC +VE+S + VYR+ R  DKSIG LEI++V+ G FD
Sbjct: 501 FWELCLDGNT----PIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 560

Query: 579 ALMDFCVSQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEP 623
            LMD+ +S G+S+NQYKTPRC+K    I++L+SR+V  ++S K P W P
Sbjct: 561 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWVP 604

[Close]

BLAST of Sed0020106 vs. TAIR 10
Match: AT1G28130.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 684.1 bits (1764), Expect = 1.1e-196
Identity = 334/462 (72.29%), Postives = 384/462 (83.12%), Query Frame = 0


Query: 172 MYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFNQYNVYTSPNETILCSDSRQSMYC 231
           MYLLFIKPEI TPSGLMARPVLTSYYKS++F NR FN+YNVYTSP++TILC DS+QSMYC
Sbjct: 1   MYLLFIKPEIKTPSGLMARPVLTSYYKSQHFRNRPFNKYNVYTSPDQTILCQDSKQSMYC 60

Query: 232 QLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKELSHNIRTGHVSDWITDPSCRAAVL 291
           QLLCGLVQR HVLRVGAVFASAFLRA+KFLEDH+KEL  +IRTG V+ WITD SCR +VL
Sbjct: 61  QLLCGLVQRSHVLRVGAVFASAFLRAVKFLEDHYKELCADIRTGTVTSWITDSSCRDSVL 120

Query: 292 LILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYIEVIVTGSMAQYISTLEFYSGGLP 351
            IL+ P+  LAD I++EC EKSWEGI++R+WPK KY+EVIVTGSMAQYI TLEFYSGGLP
Sbjct: 121 SILNGPNQELADEIESECAEKSWEGILRRIWPKAKYVEVIVTGSMAQYIPTLEFYSGGLP 180

Query: 352 LVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFFEFLPVQHQSY---------CNGG 411
           LVSTMYASSECYFGIN+NPL  P DVSYTLLPNM +FEFLPV  +S+             
Sbjct: 181 LVSTMYASSECYFGINLNPLCDPADVSYTLLPNMAYFEFLPVDDKSHEEIHFATHSNTDD 240

Query: 412 SVQTVEEFETVDLVDVKLGHYYELVVTTFTGLYRYRVGDILMVTGFHNNAPQFRFIHRRN 471
               ++E   V+LV+V++G YYE+V+TTFTGLYRYRVGDIL VTGFHN APQFRF+ RRN
Sbjct: 241 DDDALKEDLIVNLVNVEVGQYYEIVITTFTGLYRYRVGDILKVTGFHNKAPQFRFVQRRN 300

Query: 472 VVLSIDTDKTNEDDLLKAITNAKT--LLEPLGFLLTEYTSYADTGSIPGHYVLFWELKMR 531
           VVLSIDTDKT+E+DLL A+T AK   L  P   LLTEYTSYADT SIPGHYVLFWELK R
Sbjct: 301 VVLSIDTDKTSEEDLLNAVTQAKLNHLQHPSSLLLTEYTSYADTSSIPGHYVLFWELKPR 360

Query: 532 GSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEIRVVKHGAFDALMDFCV 591
            S++   +LD   ME+CCS VE  LDYVYRRCRN+DKSIG LEIRVV  G FD+LMDFCV
Sbjct: 361 HSNDP-PKLDDKTMEDCCSEVEDCLDYVYRRCRNRDKSIGPLEIRVVSLGTFDSLMDFCV 420

Query: 592 SQGSSVNQYKTPRCIKSEEAIKILDSRLVGRFYSKKMPFWEP 623
           SQGSS+NQYKTPRC+KS  A++ILDSR++GRF+SK++P WEP
Sbjct: 421 SQGSSLNQYKTPRCVKSGGALEILDSRVIGRFFSKRVPQWEP 461

[Close]

BLAST of Sed0020106 vs. TAIR 10
Match: AT2G47750.1 (putative indole-3-acetic acid-amido synthetase GH3.9 )

HSP 1 Score: 677.6 bits (1747), Expect = 9.8e-195
Identity = 338/593 (57.00%), Postives = 435/593 (73.36%), Query Frame = 0


Query: 38  NNEDGLKILEDLTENAGQIQEQVIQKILAQNLNTEYLKNFINGDSADVELFKKSVPVVNY 97
           +++  LK LE +T  A ++Q+ +++ IL +N +TEYL  ++NG S DV  FK++VP++ Y
Sbjct: 7   DHDSVLKELERITSKAAEVQDNILRGILERNKDTEYLSKYMNG-SKDVLEFKRAVPIIIY 66

Query: 98  EDIKPYVERIANGEPSHIISAQPITELLTSSGTSGGQPKMMPSTEEDLDRKTFFYNLLIP 157
           +DI PY++RIANGE S +I+   ITE+L SSGTS G+PK+MP+  EDLDR+TF YNL+IP
Sbjct: 67  KDIYPYIQRIANGEDSSLITGHSITEILCSSGTSAGEPKLMPTIPEDLDRRTFLYNLIIP 126

Query: 158 VMNKYVDGLEEGKGMYLLFIKPEISTPSGLMARPVLTSYYKSKNFTNRLFNQYNVYTSPN 217
           ++NKY+ GL++GK MYL F+K E STP GL  R VLTSYYKSK+F  R ++ +N  TSP 
Sbjct: 127 IVNKYITGLDKGKAMYLNFVKAETSTPCGLPIRAVLTSYYKSKHFQCRPYDPFNDLTSPI 186

Query: 218 ETILCSDSRQSMYCQLLCGLVQRDHVLRVGAVFASAFLRAIKFLEDHWKELSHNIRTGHV 277
           +TILC DS QSMYCQLL GL+ R  V+R+GAVFASAFLRAI +LE  W +L  +IRTG +
Sbjct: 187 QTILCEDSNQSMYCQLLAGLIHRHKVMRLGAVFASAFLRAISYLEKKWSQLCEDIRTGSL 246

Query: 278 SDWITDPSCRAAVLLILSNPDVGLADSIDAECGEKSWEGIIKRLWPKTKYIEVIVTGSMA 337
           +  ITDP C+ A+  +L +P+  LA  I+  CG  SW+GI+ +LWPK K+IE +VTGSMA
Sbjct: 247 NPMITDPGCQMAMSCLLMSPNPELASEIEEICGRSSWKGILCQLWPKAKFIEAVVTGSMA 306

Query: 338 QYISTLEFYS-GGLPLVSTMYASSECYFGININPLNKPCDVSYTLLPNMGFFEFLPVQHQ 397
           QYI  LEF+S G +PLV  MYASSE YFG+N+ PL+KP DV +TLLPNM +FEF+P+   
Sbjct: 307 QYIPALEFFSQGKIPLVCPMYASSETYFGVNVEPLSKPSDVVFTLLPNMCYFEFIPLGK- 366

Query: 398 SYCNGGSVQTVEEFE------TVDLVDVKLGHYYELVVTTFTGLYRYRVGDILMVTGFHN 457
              NG     +++ E       VDLV+VKLG YYELVVTTF GLYRYR+GD+L V GF+N
Sbjct: 367 ---NGTLSFDLDDDEQVPCDKVVDLVNVKLGRYYELVVTTFAGLYRYRIGDVLQVAGFYN 426

Query: 458 NAPQFRFIHRRNVVLSIDTDKTNEDDLLKAITNAKTLLEPLGFLLTEYTSYADTGSIPGH 517
            APQFRFI RRNVVLSID DKTNE+DL ++IT AK  L    F L EYTSYADT S+PGH
Sbjct: 427 GAPQFRFICRRNVVLSIDLDKTNEEDLHRSITLAKKKLGSNAF-LAEYTSYADTSSVPGH 486

Query: 518 YVLFWELKMRGSSENLAELDSGLMEECCSSVEQSLDYVYRRCRNKDKSIGALEIRVVKHG 577
           YVLFWE++          L+  LMEECC +VE+ LDY+YR+CR K++SIGALEIRVVK G
Sbjct: 487 YVLFWEIQ--------GHLEPKLMEECCVAVEEELDYIYRQCRTKERSIGALEIRVVKPG 546

Query: 578 AFDALMDFCVSQGSSVNQYKTPRCIKSEEA-IKILDSRLVGRFYSKKMPFWEP 623
            F+ LMD  +SQG S NQYKTPRC+KS  A  K+L+  ++  F+S + P W P
Sbjct: 547 TFEKLMDLIISQGGSFNQYKTPRCVKSNSATFKLLNGHVMASFFSPRDPTWVP 585

The following BLAST results are available for this feature:

BLAST of Sed0020106 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6581972.1	3.2e-304	86.00	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022980842.1	5.4e-304	86.00	indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita maxima]	[more]
XP_023528388.1	1.2e-303	85.83	indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita pepo subsp. pepo]	[more]
XP_022955484.1	1.3e-302	85.50	indole-3-acetic acid-amido synthetase GH3.17 [Cucurbita moschata]	[more]
XP_038900786.1	3.5e-295	82.43	indole-3-acetic acid-amido synthetase GH3.17-like [Benincasa hispida]	[more]

BLAST of Sed0020106 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FZ87	5.3e-254	70.25	Indole-3-acetic acid-amido synthetase GH3.17 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9LSQ4	5.8e-200	58.57	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
P0C0M3	7.6e-200	59.10	Probable indole-3-acetic acid-amido synthetase GH3.11 OS=Oryza sativa subsp. jap...	[more]
O81829	1.1e-198	58.57	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
O82243	1.4e-193	57.00	Putative indole-3-acetic acid-amido synthetase GH3.9 OS=Arabidopsis thaliana OX=...	[more]

BLAST of Sed0020106 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ISD7	2.6e-304	86.00	indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1GWE7	6.5e-303	85.50	indole-3-acetic acid-amido synthetase GH3.17 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1DHN6	5.7e-291	81.52	indole-3-acetic acid-amido synthetase GH3.17-like OS=Momordica charantia OX=3673...	[more]
A0A5A7TMG2	1.2e-288	82.04	Indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo var. makuwa OX...	[more]
A0A1S3BYQ7	1.2e-288	82.04	indole-3-acetic acid-amido synthetase GH3.17-like OS=Cucumis melo OX=3656 GN=LOC...	[more]

BLAST of Sed0020106 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G28130.1	3.8e-255	70.25	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	4.1e-201	58.57	Auxin-responsive GH3 family protein	[more]
AT4G27260.1	7.8e-200	58.57	Auxin-responsive GH3 family protein	[more]
AT1G28130.2	1.1e-196	72.29	Auxin-responsive GH3 family protein	[more]
AT2G47750.1	9.8e-195	57.00	putative indole-3-acetic acid-amido synthetase GH3.9	[more]

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 46..598 e-value: 3.8E-202 score: 672.8
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 32..620
None	No IPR available	PANTHER	PTHR31901:SF33	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.17	coord: 32..620

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0020106.1	Sed0020106.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016881	acid-amino acid ligase activity