Homology
BLAST of Moc03g27430 vs. NCBI nr
Match:
ALJ53304.1 (polyphenol oxidase [Luffa aegyptiaca] >APO14263.1 polyphenol oxidase 4 [Luffa aegyptiaca])
HSP 1 Score: 599.4 bits (1544), Expect = 3.5e-167
Identity = 329/552 (59.60%), Postives = 387/552 (70.11%), Query Frame = 0
Query: 56 NNGDETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDT 115
+NG +TP + SNP E G S++KFDRRDVLFGLGGLY A+L G DR ALAD ISPD
Sbjct: 38 SNGVQTP-MNSNPQE-GDSMSKFDRRDVLFGLGGLYSAAAL-GRDRSALAD---PISPDV 97
Query: 116 ANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLM 175
+C P A + +CCPP I DI DF+ PP + + RVRPAAHLIT EQLAK E+A+ M
Sbjct: 98 IHCEPITLEADEKVDCCPPSILDITDFV-PPAVENLRVRPAAHLITQEQLAKLEEAVTKM 157
Query: 176 KHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERIL 235
K L DDPR F QA+VHCAYCN AYKQ D P+ + ++HWN LFFPFHRCYLYF ERIL
Sbjct: 158 KALDPDDPRNFYQQARVHCAYCNDAYKQ-DGFPELDFDIHWNWLFFPFHRCYLYFHERIL 217
Query: 236 GYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPD--SSLYDRLRDARHVPPKLAYLNYDG-D 295
G LI+DP FAIPFWNWD+ PGM+MP IY N D S LYD LRD H PPKL LNYDG
Sbjct: 218 GSLINDPDFAIPFWNWDAIPGMQMPAIYINDDKNSPLYDELRDPNHKPPKLLDLNYDGAT 277
Query: 296 EDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWV 355
EDP +DV+ CN R M +QM + K+A GF G+ +R G+EA GMGSIE S HNN+H W
Sbjct: 278 EDPSYDVLFCNPRLMYRQMVINSKTAEGFHGKPLRAGQEAYRTGMGSIELSAHNNVHTWS 337
Query: 356 GDPNSPHNEDMGTFYSAG---MFFDSSNECMKLET--------KRRDYTDPDWLDSSFVL 415
GDP + EDMG FY+A +F+ ++ T K RD+TDPDWL++SFV
Sbjct: 338 GDPQQKYREDMGHFYAAARDPLFYAHHANVDRMWTIWQGLIDGKSRDFTDPDWLNASFVF 397
Query: 416 YDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPLAPPPEVY 475
YDENKNA+RIKV+DC+D+ LGY YQ V+IPWL+T+ IPRQ S + PP EV
Sbjct: 398 YDENKNAIRIKVQDCLDNEKLGYKYQAVEIPWLHTKPIPRQKTSQLKSSKAQETPPIEV- 457
Query: 476 FPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDV 535
LNS++ V RP RSR+ QK EDEVLVIE +E D +SRVHFNVLVNVLD+G DV
Sbjct: 458 ----LNSKMTKEVKRPQRSRSKTQKSVEDEVLVIE-MELDTTSRVHFNVLVNVLDDGTDV 517
Query: 536 SPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVT 593
SP N EFAGSF++IP H H A SL VITELIEELG DD ET+ V I PKLGG+ VT
Sbjct: 518 SPGNTEFAGSFKSIPLGHKH-RANTSLSLVITELIEELGYDDDETVFVTIDPKLGGDLVT 574
BLAST of Moc03g27430 vs. NCBI nr
Match:
XP_023542623.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 591.3 bits (1523), Expect = 9.4e-165
Identity = 304/560 (54.29%), Postives = 386/560 (68.93%), Query Frame = 0
Query: 45 IRRSHRKLASYNNG-DETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFA 104
I+ KL + + G P SN G S +KFDRRDVLFGLGGLYG A+L+ A
Sbjct: 13 IKNQTSKLQNKSRGISHVPCRASN----GESTSKFDRRDVLFGLGGLYGAAALTNQQSPA 72
Query: 105 LADDTHTISPDTANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPE 164
A ++PD NC PA CCPPKIH IIDFI PP R+RP AH +TP+
Sbjct: 73 SA---APLAPDVNNCEPATIEGNQHIPCCPPKIHHIIDFIHPPHTTKLRIRPPAHSLTPK 132
Query: 165 QLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPF 224
Q FEKAI++MK LP +DPR F QA VHCAYC+YAYKQL P AE++VHWNALFFPF
Sbjct: 133 QQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQL-GFPNAEMQVHWNALFFPF 192
Query: 225 HRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPK 284
HR YLYFFERILG+LI++P F IPFWNWD+ GM+MPKIY+NP+S LYD+LR+ RH P K
Sbjct: 193 HRAYLYFFERILGHLINEPNFVIPFWNWDNNEGMQMPKIYDNPNSPLYDKLRNPRHRPHK 252
Query: 285 LAYLNYDGDEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEA-KTAGMGSIES 344
L LNY GD++P +DVVDCNLRWM+KQM +A GF G+L+ EA + GMG+IE
Sbjct: 253 LLNLNYHGDDNPSYDVVDCNLRWMHKQMITDADTAEGFHGKLLIASHEANEETGMGTIER 312
Query: 345 SPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMKL--------ETKRRDYTD 404
SPHNN+H+WVGDPN+ ++ +MG FYSA +F+ ++ K RD+ +
Sbjct: 313 SPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKPRDFDN 372
Query: 405 PDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPE 464
PDWL+SSFV Y+E+K AVRIKV DC+D+ LGYVYQ VD+PWL+T+Q PR ++
Sbjct: 373 PDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQLKATQA 432
Query: 465 PPLAPPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVL 524
P + + FP LNS + T V RP++ R ++K E+DEVLVIE IE D+ VHFNVL
Sbjct: 433 PQV-----IKFPQGLNSMVTTTVKRPNKLRDEKEKSEKDEVLVIEGIELDKRYWVHFNVL 492
Query: 525 VNVLDEGLDVSPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVKI 584
VNV+D+G DV P N EF G+F +PH HNH K SLR VITE+++ELG++D E++ V++
Sbjct: 493 VNVVDDGSDVCPGNTEFVGTFTTVPHGHNH-RGKTSLRVVITEVLKELGIEDDESVIVRL 552
Query: 585 APKLGGENVTIGGIKIELQP 591
PK+GG+ VTIGG+KIELQP
Sbjct: 553 DPKVGGDKVTIGGMKIELQP 558
BLAST of Moc03g27430 vs. NCBI nr
Match:
XP_022994369.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])
HSP 1 Score: 587.0 bits (1512), Expect = 1.8e-163
Identity = 298/533 (55.91%), Postives = 379/533 (71.11%), Query Frame = 0
Query: 72 GISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPRN-- 131
G S +KFDRRDVLFGLGGLYG A+L+ A A ++PD NC PA T G +
Sbjct: 40 GDSTSKFDRRDVLFGLGGLYGAAALTNQQSPASA---KPLAPDVNNCEPA-TIVGNEHIP 99
Query: 132 CCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQA 191
CCPPKIHDIIDFI PP R+R AH +TP+Q FEKAI++MK L +DPR F QA
Sbjct: 100 CCPPKIHDIIDFIHPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQA 159
Query: 192 QVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWN 251
VHCAYC+YAYKQL P AE++VHWNALFFPFHR YLYFFERILG+LI++P FAIPFWN
Sbjct: 160 DVHCAYCDYAYKQL-GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWN 219
Query: 252 WDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDVVDCNLRWMNKQ 311
WD+ GM+MP+IY++P+S LYD+LR+ RH P KL LNY GD++PP+DVVDCNLRWM+KQ
Sbjct: 220 WDNKKGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQ 279
Query: 312 MKVAGKSARGFLGQLIRRGEEA-KTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSA 371
M +A GF G+L+ +EA + GMG+IE SPHNN+H+WVGDPN+ +N +MG FYSA
Sbjct: 280 MITDADTAEGFHGKLLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSA 339
Query: 372 G---MFFDSSNECMKL--------ETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVD 431
+F+ ++ K RD+ +PDWL+SSFV Y+E+K AVRIKV DC+D
Sbjct: 340 ARDPLFYGHHANVDRMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLD 399
Query: 432 SVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPS 491
+ LGYVYQ VD+PWL+T+Q PR ++ P + + FP LNS + T V RP
Sbjct: 400 TRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQV-----IKFPQGLNSMVTTTVKRPK 459
Query: 492 RSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHA 551
+ R ++K E+DEVLVIE IE D+ VHFNVLVNV+D+G DV P N EF G+F + H
Sbjct: 460 KLRDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHG 519
Query: 552 HNHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQP 591
HNH K SLR VITE+++ELG++D E++ V++ PK+GG+ VTIGG+KIELQP
Sbjct: 520 HNH-RGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561
BLAST of Moc03g27430 vs. NCBI nr
Match:
KAG6573251.1 (hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 587.0 bits (1512), Expect = 1.8e-163
Identity = 301/561 (53.65%), Postives = 388/561 (69.16%), Query Frame = 0
Query: 44 EIRRSHRKLASYNNG-DETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRF 103
+I+ L + + G + P SN D S +KFDRRDVLFGLGGLYG A+L+
Sbjct: 15 QIKNQTSNLQNKSRGINHVPCRASNGD----STSKFDRRDVLFGLGGLYGAAALTNQQSP 74
Query: 104 ALADDTHTISPDTANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITP 163
A A ++PD NC PA CCPPKIH IIDFI PP R+RP AH +TP
Sbjct: 75 ASA---APLAPDVNNCEPATIEGNQHIPCCPPKIHHIIDFIHPPHTTKLRIRPPAHSLTP 134
Query: 164 EQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFP 223
+Q FEKAI++MK LP +DPR F QA VHCAYC+YAYKQL P AE++VHWNALFFP
Sbjct: 135 KQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQL-GFPNAEMQVHWNALFFP 194
Query: 224 FHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPP 283
FHR YLYFFERILG+LI++P F IPFWNWD+ GM+MP+IY++P+S LYD+LR+ RH P
Sbjct: 195 FHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPH 254
Query: 284 KLAYLNYDGDEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEA-KTAGMGSIE 343
KL LNY GD++P +DVVD NLRWM+KQM +A GFLG+L+ +EA + GMG+IE
Sbjct: 255 KLLNLNYHGDDNPSYDVVDSNLRWMHKQMIKDADTAEGFLGKLLVASDEANEETGMGTIE 314
Query: 344 SSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMKL--------ETKRRDYT 403
SPHNN+H+WVGDPN+ ++ +MG FYSA +F+ ++ K RD+
Sbjct: 315 RSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKPRDFD 374
Query: 404 DPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSP 463
+PDWL+SSFV Y+E+K AVRIKVRDC+D+ LGYVYQ VD+PWL+T+Q PR ++
Sbjct: 375 NPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQ 434
Query: 464 EPPLAPPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNV 523
P + + FP LNS + T V RP++ ++K E+DEVLVIE IE D+ VHFNV
Sbjct: 435 APQV-----IKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNV 494
Query: 524 LVNVLDEGLDVSPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVK 583
LVNV+D+G DV P N EF G+F +PH HNH K SLR VITE+++ELG++D E++ V+
Sbjct: 495 LVNVVDDGSDVCPGNTEFVGTFTTVPHGHNH-RGKTSLRVVITEVLKELGIEDDESVIVR 554
Query: 584 IAPKLGGENVTIGGIKIELQP 591
+ PK+GG+ VTIGG+KIELQP
Sbjct: 555 LDPKVGGDKVTIGGMKIELQP 561
BLAST of Moc03g27430 vs. NCBI nr
Match:
XP_022954613.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])
HSP 1 Score: 585.9 bits (1509), Expect = 4.0e-163
Identity = 300/561 (53.48%), Postives = 387/561 (68.98%), Query Frame = 0
Query: 44 EIRRSHRKLASYNNG-DETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRF 103
+I+ L + + G ++ P SN D S +KFDRRDVLFGLGGLYG A+L+
Sbjct: 15 QIKNQTSNLQNKSRGINQVPCRASNGD----STSKFDRRDVLFGLGGLYGAAALTNQQSP 74
Query: 104 ALADDTHTISPDTANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITP 163
A A ++PD NC A CCPPKIHDIIDFI PP R+RP AH +TP
Sbjct: 75 ASA---APLAPDVNNCEAATIEGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRPPAHSLTP 134
Query: 164 EQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFP 223
+Q FEKAI++MK LP +DPR F QA VHCAYC+YAYKQL P AE++VHWNALFFP
Sbjct: 135 KQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQL-GFPNAEMQVHWNALFFP 194
Query: 224 FHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPP 283
FHR YLYFFERILG+LI++P F IPFWNWD+ GM+MP+IY++P+S LYD+LR+ RH P
Sbjct: 195 FHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPH 254
Query: 284 KLAYLNYDGDEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEA-KTAGMGSIE 343
KL LNY GD++P +DVVD NLRWM+KQM +A GF G+L+ EA + GMG+IE
Sbjct: 255 KLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEETGMGTIE 314
Query: 344 SSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMKL--------ETKRRDYT 403
SPHNN+H+WVGDPN+ ++ +MG FYSA +F+ ++ K RD+
Sbjct: 315 KSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKARDFD 374
Query: 404 DPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSP 463
+PDWL+SSFV Y+E+K AVRIKVRDC+D+ LGYVYQ VD+PWL+T+Q PR ++
Sbjct: 375 NPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQ 434
Query: 464 EPPLAPPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNV 523
P + + FP LNS + T V RP++ ++K E+DEVLVIE IE D+ VHFNV
Sbjct: 435 APQV-----IKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNV 494
Query: 524 LVNVLDEGLDVSPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVK 583
LVNV+D+G DV P N EF G+F +PH HNH K SLR VITE+++ELG++D E++ V+
Sbjct: 495 LVNVVDDGSDVCPGNTEFVGTFTTVPHGHNH-RGKTSLRVVITEVLKELGIEDDESVIVR 554
Query: 584 IAPKLGGENVTIGGIKIELQP 591
+ PK+GG+ VTIGG+KIELQP
Sbjct: 555 LDPKVGGDKVTIGGMKIELQP 561
BLAST of Moc03g27430 vs. ExPASy Swiss-Prot
Match:
P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)
HSP 1 Score: 454.1 bits (1167), Expect = 2.4e-126
Identity = 266/550 (48.36%), Postives = 340/550 (61.82%), Query Frame = 0
Query: 66 SNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPA 125
+N D+ + +K DRR+VL GLGGLYGVA + G D FA A PD + CGPA P
Sbjct: 51 TNNDQNDQAQSKLDRRNVLLGLGGLYGVAGM-GTDPFAFAKP--IAPPDVSKCGPADLPQ 110
Query: 126 G--PRNCCPPKIHDIIDF-IPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDP 185
G P NCCPP IIDF +P P R+RP AH + K+ KA+ LMK LP DDP
Sbjct: 111 GAVPTNCCPPPSTKIIDFKLPAP--AKLRIRPPAHAVDQAYRDKYYKAMELMKALPDDDP 170
Query: 186 RRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPT 245
R F QA VHCAYC+ AY Q+ P+ EL++H + LFFPFHR YLYFFE+ILG LI+DPT
Sbjct: 171 RSFKQQAAVHCAYCDGAYDQV-GFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPT 230
Query: 246 FAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDED--PPFDVVD 305
FA+PFWNWDSP GM +P IY +P S LYD+LR A H PP L L+Y+G ED ++
Sbjct: 231 FALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTIN 290
Query: 306 CNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNE 365
NL+ M +QM K+A+ F G R G+E G GSIE +PH +H W GD P+ E
Sbjct: 291 ANLKIMYRQMVSNSKNAKLFFGNPYRAGDEPDPGG-GSIEGTPHAPVHLWTGDNTQPNFE 350
Query: 366 DMGTFYSAG---MFFDSSNECMK-------LETKRRDYTDPDWLDSSFVLYDENKNAVRI 425
DMG FYSAG +FF + + L KR D TD DWLDS F+ Y+EN VR+
Sbjct: 351 DMGNFYSAGRDPIFFAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRV 410
Query: 426 KVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPL-------APPPEVY--- 485
KVRDC+++ NLGYVYQDVDIPWL+++ PR+ S L A +V
Sbjct: 411 KVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGT 470
Query: 486 -FPAMLNSEINTVVGRP-SRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGL 545
FP L S+I+TVV RP + R+ + K +E+E+LVIE IEFDR V F+V VN +D+ L
Sbjct: 471 EFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDD-L 530
Query: 546 DVSPANIEFAGSFRNIPHAHNHNNAKNS-LRFVITELIEELGLDDAETLHVKIAPKLGGE 588
P EFAGSF ++PH+H H N+ LR +T+L+EE+ +D +++ V + PK G
Sbjct: 531 PSGPDKTEFAGSFVSVPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFGA- 590
BLAST of Moc03g27430 vs. ExPASy Swiss-Prot
Match:
P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)
HSP 1 Score: 442.2 bits (1136), Expect = 9.3e-123
Identity = 264/581 (45.44%), Postives = 340/581 (58.52%), Query Frame = 0
Query: 32 FKIVAEGPAIGDEIRRSHRKLASYNNGDETPIIKSNPDEVGISVNKFDRRDVLFGLGGLY 91
F+ + P + RR S N+ + P S D S K DRR+VL G+GGLY
Sbjct: 29 FQRASHVPVARNRSRRFAPSKVSCNSANGDPNSDSTSDVRETSSGKLDRRNVLLGIGGLY 88
Query: 92 GVASLSGGDRFALADDTHTISPDTANCGPARTPAG--PRNCCPPKIHDIIDFIPPPMIPH 151
G A G + LA +PD + CG A P G P NCCPP IIDF P
Sbjct: 89 GAAGGLGATK-PLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDFQLPSSGSP 148
Query: 152 PRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKA 211
R RPAAHL++ E LAK++KAI L K LP DDPR F QA VHC YC AY Q+
Sbjct: 149 MRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQV-GYTDL 208
Query: 212 ELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSL 271
EL+VH + LF PFHR YLYF ERIL LIDDPTFA+P+W WD+P GM MP IY + SSL
Sbjct: 209 ELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSPSSL 268
Query: 272 YDRLRDARHVPPKLAYLNYDGDED--PPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRR 331
YD R+A+H+PP + L+YDG E P ++ NL M KQ+ + + FLG R
Sbjct: 269 YDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFLGYPYRA 328
Query: 332 GEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMKL--- 391
G +A G G++E +PHN +H W G + P +EDMG FY+AG +FF ++
Sbjct: 329 G-DAIDPGAGTLEHAPHNIVHKWTGLADKP-SEDMGNFYTAGRDPIFFGHHANVDRMWNI 388
Query: 392 -----ETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWL--N 451
R+D+TD DWLD++FV YDENK V++KV DCVD+ L Y YQD+ IPWL N
Sbjct: 389 WKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLPKN 448
Query: 452 TRQIPRQNPPTSSSPEPPLAPPPEVY------FPAMLNSEINTVVGRPSRSRTIEQKGEE 511
T+ + +S S A P+ FP LNS I V RP +SR+ ++K +E
Sbjct: 449 TKAKAKTTTKSSKSGVAKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRSKKEKEDE 508
Query: 512 DEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVS-PANIEFAGSFRNIPHAH-NHNNAKNS 571
+EVL+I+ IE DR + V F+V +N DE VS P N EFAGSF N+PH H K +
Sbjct: 509 EEVLLIKGIELDRENFVKFDVYIN--DEDYSVSRPKNSEFAGSFVNVPHKHMKEMKTKTN 568
Query: 572 LRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIE 588
LRF I EL+E+LG +D E++ V I P+ GG++VTIGGI+IE
Sbjct: 569 LRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIE 603
BLAST of Moc03g27430 vs. ExPASy Swiss-Prot
Match:
Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)
HSP 1 Score: 398.3 bits (1022), Expect = 1.5e-109
Identity = 235/557 (42.19%), Postives = 327/557 (58.71%), Query Frame = 0
Query: 53 ASYNNGDETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTH-TI 112
AS +N D K N E S+ DRR++L GLGGLYG +++ ++ LA +
Sbjct: 35 ASNDNKD-----KPNDQEKTFSI---DRRNMLIGLGGLYGASNVFPSNQSTLAAPIQPPV 94
Query: 113 SPDTANCGPARTPAGPRN--CCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPEQLAKFEK 172
PD +C P A N CCPP + D IDF P R+RPAAHL P + K++K
Sbjct: 95 LPD--SCHPPEDLAEGVNVLCCPPDVKDPIDFQMPSNPSRLRIRPAAHLADPTYIEKYKK 154
Query: 173 AIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYF 232
A+ MK LP +DPR F QA +HCAYCN AY Q+ P ++VH + LF PFHR YLYF
Sbjct: 155 ALAAMKALPQNDPRSFYQQANIHCAYCNGAYDQV-GFPDVNIQVHHSWLFLPFHRWYLYF 214
Query: 233 FERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYD 292
+ERILG LIDDPTFAIPFWNWD+P GM MP ++ +P+S LYD R+ H P + L++
Sbjct: 215 YERILGSLIDDPTFAIPFWNWDAPKGMHMPHMFIDPNSPLYDAKRNPAHFPDTIVDLDFS 274
Query: 293 GDEDPPFD--VVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNL 352
E P + + NL M KQ+ V K AR F G+ ++ G +G GS+E +PH N+
Sbjct: 275 SGEAPSHNPRQIGNNLSIMYKQV-VRAKKARLFHGRPLQAGSFPDESGDGSLEGTPHGNI 334
Query: 353 HDWVGDPNSPHNEDMGTFYSAG---MFF------DSSNECMKLETKRRDYTDPDWLDSSF 412
H W GDP + E+MG FYSAG +F+ D K R+DY DWL++ F
Sbjct: 335 HLWSGDPRQSNFENMGNFYSAGRDPLFYAHHANVDRMWYIWKNSLGRKDYKKKDWLNAGF 394
Query: 413 VLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPLAPPPE 472
+L+DEN VR+ VRD +D LGY YQ+VDIPW+N++ PR+ P + +
Sbjct: 395 LLFDENAQPVRVYVRDALDERKLGYAYQEVDIPWINSKPKPRKANPWPNRLRSKATTTTK 454
Query: 473 VY--FPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDE 532
+ FP L+S ++ V RP +SR+ +K +E+EVLVIE I+ + + F+V +N DE
Sbjct: 455 LINKFPLTLDSTVSFEVKRPKKSRSKSEKEDEEEVLVIEKIKHEPQFPLKFDVYIN--DE 514
Query: 533 GLDVSPAN-IEFAGSFRNIPHAHNHNNAKN-----SLRFVITELIEELGLDDAETLHVKI 588
D S A+ EFAGSF N+PH H H + K+ +L I+E+++EL +D +++ V +
Sbjct: 515 DEDPSAADQTEFAGSFVNVPHFHRHGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTL 574
BLAST of Moc03g27430 vs. ExPASy Swiss-Prot
Match:
Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)
HSP 1 Score: 397.9 bits (1021), Expect = 2.0e-109
Identity = 247/580 (42.59%), Postives = 339/580 (58.45%), Query Frame = 0
Query: 44 EIRRSHRKLASYNNGDETPIIKSNP-DEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRF 103
++R+ R++ +N ++ +NP +E +S RR+VL GLGG+YG + +
Sbjct: 38 KLRKPKRQVTCSSNNNQ-----NNPKEEQELSNIVGHRRNVLIGLGGIYGTLATNPS--- 97
Query: 104 ALADDTHTISPDTANC-GPARTPAGPR----NCCPPKIHDIIDFIPPPMIPHPRVRPAAH 163
ALA PD + C P+ P+G NCCPP I DF P P RVR AAH
Sbjct: 98 ALASPIS--PPDLSKCVPPSDLPSGTTPPNINCCPPYSTKITDFKFPSNQP-LRVRQAAH 157
Query: 164 LITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNA 223
L+ E L K++KA LMK LP +DPR F+ QA +HCAYC+ AY Q+ P +L+VH +
Sbjct: 158 LVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQI-GFPDLKLQVHGSW 217
Query: 224 LFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDAR 283
LFFPFHR YLYF+ERILG LI+DPTFA+PFWN+D+P GM++P IY + S LYD LR+A
Sbjct: 218 LFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPLYDELRNAS 277
Query: 284 HVPPKLAYLNY--DGDEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAG 343
H PP L LN+ G + +++ NL M +Q+ GK++R FLG R G +A+ G
Sbjct: 278 HQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYRAG-DAEPQG 337
Query: 344 MGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMK-------LETKR 403
GSIE+ PH +H W GD + EDMG FYSA +F+ + + L K+
Sbjct: 338 AGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYIWKTLGGKK 397
Query: 404 RDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPT 463
D+TD DWL+S F+ YDENKN VR+ V+D +D LGY YQDV IPW + +PR+
Sbjct: 398 HDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKAKPVPRRTKVQ 457
Query: 464 S------------SSPEPPL----APPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEED 523
SP L +P V FP +LN++++ +V RP + R+ ++K EE+
Sbjct: 458 KLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKKLRSKKEKEEEE 517
Query: 524 EVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPH-AHNHNNAK--NS 583
EVLVIE IEF + + F+V +N DE V N EFAGSF NIPH AH H N K S
Sbjct: 518 EVLVIEGIEFYMNIAIKFDVYIN--DEDDKVGAGNTEFAGSFVNIPHSAHGHKNKKIITS 577
Query: 584 LRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKI 587
LR IT+L+E+L ++ + + V + PK G V I ++I
Sbjct: 578 LRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEI 602
BLAST of Moc03g27430 vs. ExPASy Swiss-Prot
Match:
Q9FRX6 (Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1)
HSP 1 Score: 393.3 bits (1009), Expect = 4.9e-108
Identity = 227/521 (43.57%), Postives = 309/521 (59.31%), Query Frame = 0
Query: 89 GLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAG--PRNCCPPKIHDIIDFIPPPM 148
GLY SL+ RFA +PD CG P G P NCCPP IIDF PP
Sbjct: 43 GLYIANSLAYA-RFASTSTGPIAAPDVTKCGQPDLPPGTAPINCCPPIPAKIIDFELPPP 102
Query: 149 IPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSV 208
RVR AAHL+ +AKF+KA+ LM+ LP DDPR F QA VHCAYC AY Q
Sbjct: 103 STTMRVRRAAHLVDDAYIAKFKKAVELMRALPEDDPRSFKQQANVHCAYCAGAYNQA-GF 162
Query: 209 PKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPD 268
+L++H + LFFPFHR Y+YFFERILG LI+D TFA+PFWN+DSP GM +P ++ + +
Sbjct: 163 TNLKLQIHRSWLFFPFHRYYIYFFERILGKLINDTTFALPFWNYDSPGGMTIPSMFIDTN 222
Query: 269 SSLYDRLRDARHVPPKLAYLNY---DGDE-DPPFDVVDCNLRWMNKQMKVAGKSARGFLG 328
SSLYD LRD+ H PP + LNY D D P + + NL+ + +QM + K+ + F G
Sbjct: 223 SSLYDSLRDSNHQPPTIVDLNYAFSDSDNTTTPEEQMIINLKIVYRQMVSSAKTPQLFFG 282
Query: 329 QLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECM 388
+ RRG++ + G+GSIE PH +H W G N+P+ E+MG FYS +FF +
Sbjct: 283 RPYRRGDQ-EFPGVGSIELVPHGMIHLWTGSENTPYGENMGAFYSTARDPIFFAHHSNVD 342
Query: 389 KLET--------KRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIP 448
++ + +R D TDPD+LD+SFV YDEN VR+KVRDC+D LGYVYQDV+IP
Sbjct: 343 RMWSIWKTLGGPRRTDLTDPDFLDASFVFYDENAEMVRVKVRDCLDEKKLGYVYQDVEIP 402
Query: 449 WLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEV 508
WLNTR P+ +P P FPA+L+ + +V RP ++R+ ++K E +E+
Sbjct: 403 WLNTRPTPKVSPSLLKKFHRTNTANPRQVFPAILDRVLKVIVTRPKKTRSRKEKDELEEI 462
Query: 509 LVIEDIEFDRS-SRVHFNVLVNVLDEGLDV-SPANIEFAGSFRNIPH--AHNHNNAKNSL 568
LVIE IE +R V F+V +N ++ L V SP N EFAGSF ++ H L
Sbjct: 463 LVIEGIELERDHGHVKFDVYINADEDDLAVISPENAEFAGSFVSLWHKPIKGKRTKTQLL 522
Query: 569 RFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIEL 589
I +++E+L D+ + + V + P+ G+ + I +KIEL
Sbjct: 523 TLSICDILEDLDADEDDYVLVTLVPRNAGDAIKIHNVKIEL 560
BLAST of Moc03g27430 vs. ExPASy TrEMBL
Match:
A0A172DC01 (Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1)
HSP 1 Score: 599.4 bits (1544), Expect = 1.7e-167
Identity = 329/552 (59.60%), Postives = 387/552 (70.11%), Query Frame = 0
Query: 56 NNGDETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDT 115
+NG +TP + SNP E G S++KFDRRDVLFGLGGLY A+L G DR ALAD ISPD
Sbjct: 38 SNGVQTP-MNSNPQE-GDSMSKFDRRDVLFGLGGLYSAAAL-GRDRSALAD---PISPDV 97
Query: 116 ANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLM 175
+C P A + +CCPP I DI DF+ PP + + RVRPAAHLIT EQLAK E+A+ M
Sbjct: 98 IHCEPITLEADEKVDCCPPSILDITDFV-PPAVENLRVRPAAHLITQEQLAKLEEAVTKM 157
Query: 176 KHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERIL 235
K L DDPR F QA+VHCAYCN AYKQ D P+ + ++HWN LFFPFHRCYLYF ERIL
Sbjct: 158 KALDPDDPRNFYQQARVHCAYCNDAYKQ-DGFPELDFDIHWNWLFFPFHRCYLYFHERIL 217
Query: 236 GYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPD--SSLYDRLRDARHVPPKLAYLNYDG-D 295
G LI+DP FAIPFWNWD+ PGM+MP IY N D S LYD LRD H PPKL LNYDG
Sbjct: 218 GSLINDPDFAIPFWNWDAIPGMQMPAIYINDDKNSPLYDELRDPNHKPPKLLDLNYDGAT 277
Query: 296 EDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWV 355
EDP +DV+ CN R M +QM + K+A GF G+ +R G+EA GMGSIE S HNN+H W
Sbjct: 278 EDPSYDVLFCNPRLMYRQMVINSKTAEGFHGKPLRAGQEAYRTGMGSIELSAHNNVHTWS 337
Query: 356 GDPNSPHNEDMGTFYSAG---MFFDSSNECMKLET--------KRRDYTDPDWLDSSFVL 415
GDP + EDMG FY+A +F+ ++ T K RD+TDPDWL++SFV
Sbjct: 338 GDPQQKYREDMGHFYAAARDPLFYAHHANVDRMWTIWQGLIDGKSRDFTDPDWLNASFVF 397
Query: 416 YDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPLAPPPEVY 475
YDENKNA+RIKV+DC+D+ LGY YQ V+IPWL+T+ IPRQ S + PP EV
Sbjct: 398 YDENKNAIRIKVQDCLDNEKLGYKYQAVEIPWLHTKPIPRQKTSQLKSSKAQETPPIEV- 457
Query: 476 FPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDV 535
LNS++ V RP RSR+ QK EDEVLVIE +E D +SRVHFNVLVNVLD+G DV
Sbjct: 458 ----LNSKMTKEVKRPQRSRSKTQKSVEDEVLVIE-MELDTTSRVHFNVLVNVLDDGTDV 517
Query: 536 SPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVT 593
SP N EFAGSF++IP H H A SL VITELIEELG DD ET+ V I PKLGG+ VT
Sbjct: 518 SPGNTEFAGSFKSIPLGHKH-RANTSLSLVITELIEELGYDDDETVFVTIDPKLGGDLVT 574
BLAST of Moc03g27430 vs. ExPASy TrEMBL
Match:
A0A6J1K2N9 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111490108 PE=3 SV=1)
HSP 1 Score: 587.0 bits (1512), Expect = 8.6e-164
Identity = 298/533 (55.91%), Postives = 379/533 (71.11%), Query Frame = 0
Query: 72 GISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPRN-- 131
G S +KFDRRDVLFGLGGLYG A+L+ A A ++PD NC PA T G +
Sbjct: 40 GDSTSKFDRRDVLFGLGGLYGAAALTNQQSPASA---KPLAPDVNNCEPA-TIVGNEHIP 99
Query: 132 CCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQA 191
CCPPKIHDIIDFI PP R+R AH +TP+Q FEKAI++MK L +DPR F QA
Sbjct: 100 CCPPKIHDIIDFIHPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQA 159
Query: 192 QVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWN 251
VHCAYC+YAYKQL P AE++VHWNALFFPFHR YLYFFERILG+LI++P FAIPFWN
Sbjct: 160 DVHCAYCDYAYKQL-GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWN 219
Query: 252 WDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDVVDCNLRWMNKQ 311
WD+ GM+MP+IY++P+S LYD+LR+ RH P KL LNY GD++PP+DVVDCNLRWM+KQ
Sbjct: 220 WDNKKGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQ 279
Query: 312 MKVAGKSARGFLGQLIRRGEEA-KTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSA 371
M +A GF G+L+ +EA + GMG+IE SPHNN+H+WVGDPN+ +N +MG FYSA
Sbjct: 280 MITDADTAEGFHGKLLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSA 339
Query: 372 G---MFFDSSNECMKL--------ETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVD 431
+F+ ++ K RD+ +PDWL+SSFV Y+E+K AVRIKV DC+D
Sbjct: 340 ARDPLFYGHHANVDRMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLD 399
Query: 432 SVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPS 491
+ LGYVYQ VD+PWL+T+Q PR ++ P + + FP LNS + T V RP
Sbjct: 400 TRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQV-----IKFPQGLNSMVTTTVKRPK 459
Query: 492 RSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHA 551
+ R ++K E+DEVLVIE IE D+ VHFNVLVNV+D+G DV P N EF G+F + H
Sbjct: 460 KLRDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHG 519
Query: 552 HNHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQP 591
HNH K SLR VITE+++ELG++D E++ V++ PK+GG+ VTIGG+KIELQP
Sbjct: 520 HNH-RGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561
BLAST of Moc03g27430 vs. ExPASy TrEMBL
Match:
A0A6J1GRK5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456826 PE=3 SV=1)
HSP 1 Score: 585.9 bits (1509), Expect = 1.9e-163
Identity = 300/561 (53.48%), Postives = 387/561 (68.98%), Query Frame = 0
Query: 44 EIRRSHRKLASYNNG-DETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRF 103
+I+ L + + G ++ P SN D S +KFDRRDVLFGLGGLYG A+L+
Sbjct: 15 QIKNQTSNLQNKSRGINQVPCRASNGD----STSKFDRRDVLFGLGGLYGAAALTNQQSP 74
Query: 104 ALADDTHTISPDTANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITP 163
A A ++PD NC A CCPPKIHDIIDFI PP R+RP AH +TP
Sbjct: 75 ASA---APLAPDVNNCEAATIEGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRPPAHSLTP 134
Query: 164 EQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFP 223
+Q FEKAI++MK LP +DPR F QA VHCAYC+YAYKQL P AE++VHWNALFFP
Sbjct: 135 KQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQL-GFPNAEMQVHWNALFFP 194
Query: 224 FHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPP 283
FHR YLYFFERILG+LI++P F IPFWNWD+ GM+MP+IY++P+S LYD+LR+ RH P
Sbjct: 195 FHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPH 254
Query: 284 KLAYLNYDGDEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEA-KTAGMGSIE 343
KL LNY GD++P +DVVD NLRWM+KQM +A GF G+L+ EA + GMG+IE
Sbjct: 255 KLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEETGMGTIE 314
Query: 344 SSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMKL--------ETKRRDYT 403
SPHNN+H+WVGDPN+ ++ +MG FYSA +F+ ++ K RD+
Sbjct: 315 KSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKARDFD 374
Query: 404 DPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSP 463
+PDWL+SSFV Y+E+K AVRIKVRDC+D+ LGYVYQ VD+PWL+T+Q PR ++
Sbjct: 375 NPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQ 434
Query: 464 EPPLAPPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNV 523
P + + FP LNS + T V RP++ ++K E+DEVLVIE IE D+ VHFNV
Sbjct: 435 APQV-----IKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNV 494
Query: 524 LVNVLDEGLDVSPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVK 583
LVNV+D+G DV P N EF G+F +PH HNH K SLR VITE+++ELG++D E++ V+
Sbjct: 495 LVNVVDDGSDVCPGNTEFVGTFTTVPHGHNH-RGKTSLRVVITEVLKELGIEDDESVIVR 554
Query: 584 IAPKLGGENVTIGGIKIELQP 591
+ PK+GG+ VTIGG+KIELQP
Sbjct: 555 LDPKVGGDKVTIGGMKIELQP 561
BLAST of Moc03g27430 vs. ExPASy TrEMBL
Match:
A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)
HSP 1 Score: 573.5 bits (1477), Expect = 9.9e-160
Identity = 304/554 (54.87%), Postives = 382/554 (68.95%), Query Frame = 0
Query: 51 KLASYNNGDETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHT 110
K AS NNG++ SV+KFDRR+VLFGLGGLYGV + +G A
Sbjct: 37 KAASSNNGED-------------SVSKFDRREVLFGLGGLYGVVTATGSVLAA------P 96
Query: 111 ISPDTANCGPARTPAGPRNCCPPKIHDIIDFIPPPMIPHPRV-RPAAHLITPEQLAKFEK 170
++PD NC A+TP G +NCCPP+I II F+ PP I P V RPAAHL+ Q+ + E+
Sbjct: 97 LTPDVFNCDMAQTPGGIKNCCPPRIGKIIHFV-PPKIQQPYVRRPAAHLMNQTQIEELER 156
Query: 171 AIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYF 230
I +K L DDP FS+QA++HCAYC+YAY QL S+ + EVH NALFFPFHR YLYF
Sbjct: 157 GIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYNQLGSITETRFEVHSNALFFPFHRAYLYF 216
Query: 231 FERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYD 290
FERIL + I +P FAIPFWNWDSPPGM MP+ YN P SSLYD LRD RH P KL LNY+
Sbjct: 217 FERILRHYIGNPDFAIPFWNWDSPPGMHMPEFYNRPSSSLYDNLRDPRHDPHKLIDLNYN 276
Query: 291 G-DEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLH 350
G ED FDVVDCNLRWMN +MKV +S R FLG++++ GE K + MG+IE SPHNN+H
Sbjct: 277 GKTEDHSFDVVDCNLRWMNDRMKV--QSPRSFLGRVLKAGETPKPSDMGTIEQSPHNNIH 336
Query: 351 DWVGDPNSPHNEDMGTFYSAG---MFFDSS-------NECMKLETKRRDYTDPDWLDSSF 410
DWVG +PH EDMG+FYSA +F+ N L+ +D+TDPDWL+SSF
Sbjct: 337 DWVG-TKTPHFEDMGSFYSAALDPLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNSSF 396
Query: 411 VLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNP-PTSSSPEPPLAPPP 470
V YDENKNAVRI+V DC+D+ LGYVYQ+V +PWL +IP NP + PEPP+
Sbjct: 397 VFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPI---- 456
Query: 471 EVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEG 530
+ FP LNS+I T+V RP + + E E D VLV+ED+E+D ++RVHF+VLVNV+DE
Sbjct: 457 -INFPQTLNSDITTMVDRPEEAGSGE---EVDVVLVLEDVEYDPTTRVHFDVLVNVVDEA 516
Query: 531 LDVSPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGE 590
D A +EF GSF+NIPH +H++ K SLRFVITE+I+ LGL+ + L VKI P +GGE
Sbjct: 517 QDRCAAFLEFVGSFKNIPHGLHHHD-KTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGE 558
Query: 591 NVTIGGIKIELQPI 592
VT+GGIKI+L+P+
Sbjct: 577 GVTLGGIKIQLEPV 558
BLAST of Moc03g27430 vs. ExPASy TrEMBL
Match:
A0A1S3BFJ5 (polyphenol oxidase I, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489300 PE=3 SV=1)
HSP 1 Score: 570.5 bits (1469), Expect = 8.3e-159
Identity = 298/531 (56.12%), Postives = 374/531 (70.43%), Query Frame = 0
Query: 74 SVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPRNCCPP 133
SV+KFDRR+VLFGLGGLYGV + +G A ++PD NC A+TP G +NCCPP
Sbjct: 212 SVSKFDRREVLFGLGGLYGVVTATGSVLAA------PLTPDVFNCDMAQTPGGIKNCCPP 271
Query: 134 KIHDIIDFIPPPMIPHPRV-RPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVH 193
+I II F+ PP I P V RPAAHL+ Q+ + E+ I +K L DDP FS+QA++H
Sbjct: 272 RIGKIIHFV-PPKIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIH 331
Query: 194 CAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDS 253
CAYC+YAY QL S+ + EVH NALFFPFHR YLYFFERIL + I +P FAIPFWNWDS
Sbjct: 332 CAYCDYAYNQLGSITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDS 391
Query: 254 PPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDG-DEDPPFDVVDCNLRWMNKQMK 313
PPGM MP+ YN P SSLYD LRD RH P KL LNY+G ED FDVVDCNLRWMN +MK
Sbjct: 392 PPGMHMPEFYNRPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMK 451
Query: 314 VAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAG-- 373
V +S R FLG++++ GE K + MG+IE SPHNN+HDWVG +PH EDMG+FYSA
Sbjct: 452 V--QSPRSFLGRVLKAGETPKPSDMGTIEQSPHNNIHDWVG-TKTPHFEDMGSFYSAALD 511
Query: 374 -MFFDSS-------NECMKLETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNL 433
+F+ N L+ +D+TDPDWL+SSFV YDENKNAVRI+V DC+D+ L
Sbjct: 512 PLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKL 571
Query: 434 GYVYQDVDIPWLNTRQIPRQNP-PTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSR 493
GYVYQ+V +PWL +IP NP + PEPP+ + FP LNS+I T+V RP +
Sbjct: 572 GYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPI-----INFPQTLNSDITTMVDRPEEAG 631
Query: 494 TIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHNH 553
+ E E D VLV+ED+E+D ++RVHF+VLVNV+DE D A +EF GSF+NIPH +H
Sbjct: 632 SGE---EVDVVLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHH 691
Query: 554 NNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQPI 592
++ K SLRFVITE+I+ LGL+ + L VKI P +GGE VT+GGIKI+L+P+
Sbjct: 692 HD-KTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 723
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| ALJ53304.1 | 3.5e-167 | 59.60 | polyphenol oxidase [Luffa aegyptiaca] >APO14263.1 polyphenol oxidase 4 [Luffa ae... | [more] |
| XP_023542623.1 | 9.4e-165 | 54.29 | polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo] | [more] |
| XP_022994369.1 | 1.8e-163 | 55.91 | polyphenol oxidase, chloroplastic-like [Cucurbita maxima] | [more] |
| KAG6573251.1 | 1.8e-163 | 53.65 | hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sorori... | [more] |
| XP_022954613.1 | 4.0e-163 | 53.48 | polyphenol oxidase, chloroplastic-like [Cucurbita moschata] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| P43309 | 2.4e-126 | 48.36 | Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1 | [more] |
| P43311 | 9.3e-123 | 45.44 | Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1 | [more] |
| Q6UIL3 | 1.5e-109 | 42.19 | (+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 S... | [more] |
| Q06215 | 2.0e-109 | 42.59 | Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1 | [more] |
| Q9FRX6 | 4.9e-108 | 43.57 | Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A172DC01 | 1.7e-167 | 59.60 | Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1 | [more] |
| A0A6J1K2N9 | 8.6e-164 | 55.91 | polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114901... | [more] |
| A0A6J1GRK5 | 1.9e-163 | 53.48 | polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11145... | [more] |
| A0A5A7UM88 | 9.9e-160 | 54.87 | Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861... | [more] |
| A0A1S3BFJ5 | 8.3e-159 | 56.12 | polyphenol oxidase I, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489300... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
