Moc01g19860 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc01g19860
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPyruvate decarboxylase
Locationchr1: 13879682 .. 13883049 (-)
RNA-Seq ExpressionMoc01g19860
SyntenyMoc01g19860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCACCGACGCATCGACGCTAGGCCACTATCTCGGCCGCCGCCTCGTTGAAATCGGCGTTTCCGATATTTTTGCCGTTCCGGGAGACTCCATTCTTTCACTACTCGACTATTTCGCGGCGGAGCAAGGCCTGAATCTCGTCGGCTGCTGCAGCGAGCTGAATGCCGGCTACGCGGCTGATGGCTACGCCAGGCGCCGCGGCGTCGGAGCCTGCGCTGTCACCTTCACCGTCGGCGGCCTCAGCCTGATCAACGCCGTCGCCGGAGCTTACTCCGAGGACCTTCCGGTGATTTGTATTGTGGGTGGGCCCAACTCCAATTATTATGGGACTAAAAACATTCTCCACCATACCATTGGATCCCCCGATTTCAGTCAAGAACTCCGCTGCTTCCGAAATTTTACCTGTTATCAGGTTGTATTAGAAGCCCCCAAAAAATGTTTTAATTTTTTAGAAAATTTTCAAACCACTTATTATTATTAAATTAATTTTTTTAATGTTTTTAATCCGGACAAACATATTTTTTACCCAATTTAACCAAACACTACGATTCCTATTTCTATTTTTATTATTATTAACAAGCACTTTTCATCATTCATGAATTCTATAAAAAAGAGTACCTTAGCTTTAGTGCATCATTAACCAACAATAATTAAACATCCTTCTCAAGAACAATTATGGATATGCATGAAAGTAAAACTAACATCCAAAACTAAAATAGATTTATGTAGATCTATAATCGGGTGGACGTGTCTCTTTATCAACTATGTAAATAGTGTTCGATTGGTAAGGAATGCAAACAAAGTCGCATGTTGGTTGGGAATGGATAATATCCGTGGTATATAAGAATGAGATCTTAATTGATATTACGTCTTTTGGGTGAAATCAAAAGCAAAATAGTGAAGTTATGCCCAAAACAAACAATATTATATTATTGTTCAAACAAGAGAGTATCGAAGTTTAAGAAGAAATATCATATAGATCATCAAAACTAAAGAACATGTTTATAAAATTATCGAGATATAATAATTGTTCTTTAATTTATTACTCAACGATGAATTAACATAAAATTAAATATTCCATGTATTGGTTTGTTTTTGGTCTTGTTGTGGTGGTGTTAGGCCATTATTGAGAGTGTGGAGGAGGCACAGTGGCAGATAGACACAGCAATTTGTAAATGTTTGGAAGAGAGCAAACCAGTTTACATCAGCATTTGCTGCAATTTGGTCGACATTCCTCATCCTTCCTTCTCAATGCAACCACTCATCCCACTCTCCATCTCTCCCAAGTAATTCATTTCTCTATCACATCAATCAAAAAGAGCTACTTACAAAACATATAATATATCTTTTAGTTATAAAATAGTTTTATCTATGAACTAACAATTTAGTCTATTTAATCTTTTAATTTGTAATAATTTTGTCATGCGAATTTAAATTTGTAGTAATTTAGTTCCTACACTTCAATATGTAATCATTTGGTCTGTCTAATCCAAAACTTATTACAAGTTAGTCCCATAGTGAAAAATATTGTTGAAATTTAATGAGGTTTGGTATATATAAACTGATAAACCGATGAGAATGACCAAATCAATTTATTATTACAAACTACAAAGATTAAGTCGTTGACTAAAATTTTACAAATTTTAACCATCATAGGTGGTCCGTGGATCGAAATAGACTAAGTATAATATAAGAGAGAATTAGTTCAAATCCTATGATGAATATTTATTTTAGAAAGTAATTTTTATATGTTTCCTTAGAAACAAATGTGGTAAGGTTGGACAGTTGTCCCTGAGAGATATCAAAAAGAAAAAAAGGAAGTTACAAATTTTAAATTATAATGAATAAATTGTTACCGCATAAACTAAATATTATAAAAATTTTGAAATTATTAGTTAAATTAAATGGAAGAATAGGGTATTAATTTCTCAGAACGATCTCACAGGGAAAGTAACCAGATGGGTTTGGAGGTGGCAGTGGCCCAGGCGGCGGAGCTGCTAAATAGCGCCGTAAAACCAGTGATGGTCGGCGGGAGGAAGCTGAGACCCGCCGGAGCCGGGGCGGCGTTCCTCGACCTCGCCGACGCTTGTGGCTATGCCGTCGCCGTTACGCCGTCGGCGAAAGGCCTGTTTCCGGAGACTCACCCTCACTTCATCGGAACTTACTGGGGAGCCATCAGCTCCGCCGTCTGCCGCGAAACGGTGGAAATAGCCGACGCCTCGCTCTTCGCCGGCCCCAATTTCGACGACGTCGAAACGCTCGGCTTCTCGCTTGCCTACAAGAAGAACAAGGCCATTATTGTAGAGCCCGAACGCGTTGTGTTCCCTAATGGGCCGCGTTTTGGGCCCATTTTAATGAAGGACTTCCTTTGGGCTTTGGCCAAGCAACTGAAGCCCAACCCAACTGCTTATGAGAATTATCGCCGGATTTATATTCCGGAGTCGGGTCACGTCCAGTCGGAGCCGGGCGAGGCCTTGCGGGTCAACGTTCTTTTCAAACATATCCGAAAGATGTTAATGAGTGACATGACTGTCATATCTGAAAGCGGTGACTCTTGGTTTCACTGTCAAAAATTGAAATTACCACAATCATGCGGGTAAAGTAAAACCCACTATCTTTCTTGACATGTAAATATGCTAAATAGTCTTTTTAACATTGAATTATAGAAAAATATAAATATTGCAGGTACGAGGTGCAATTGCTATATGGATCAATTGGATGGTCTCTAGGTGCAACTCTTGGTTATGCACAAGCCGCACCAGAAAAGCGAGTGGTTCTTTTTATCGGTGATGGGAGCTTTCAGGTCTAATTTATTCTAGTTTAGCATTTTTTTTAATGTTTTATTGAAAAAATTTATAAACTAATTAAAATGAAAACATATGTAAATGATTGATCATATTTGCGATGGGATAGATGTGTCCACAAGATGTATCGACAATGTTAAGATTGGGACAAAGAAATATAATATTTTTGATAAATAATGGTGGATACACCCTTCAAGTAGAGTTTCACGATGGTCCTTATACTGTCATCAAGAATTGGGACTACACTCTTCTCGTTGATGCTATGAACAATCATGTTGGCAAATGTTGGACTACCAAGGTAAAATCTCAATATTTTTTTCATATATATATAGTGGCATTACTTTATATTTTTAATGAGTTATTTTTTTTATAAATTTTAAATTTTTATCTCAAGGTTGAAACGGAAGCAGAGTTAGTGCAAGCAATTGAAACATCAATGAGAGATCAAAATGATTGTTTGTGCTTCATAGAAGCTATTGTTCATAGAGATGATACAAGTAAAGAGTTACTTGAATTTGGGAACACCCTTGATGCTATCATTAGTCGTCCTCCCAAACTTTAG

mRNA sequence

ATGGCCACCGACGCATCGACGCTAGGCCACTATCTCGGCCGCCGCCTCGTTGAAATCGGCGTTTCCGATATTTTTGCCGTTCCGGGAGACTCCATTCTTTCACTACTCGACTATTTCGCGGCGGAGCAAGGCCTGAATCTCGTCGGCTGCTGCAGCGAGCTGAATGCCGGCTACGCGGCTGATGGCTACGCCAGGCGCCGCGGCGTCGGAGCCTGCGCTGTCACCTTCACCGTCGGCGGCCTCAGCCTGATCAACGCCGTCGCCGGAGCTTACTCCGAGGACCTTCCGGTGATTTGTATTGTGGGTGGGCCCAACTCCAATTATTATGGGACTAAAAACATTCTCCACCATACCATTGGATCCCCCGATTTCAGTCAAGAACTCCGCTGCTTCCGAAATTTTACCTGTTATCAGGCCATTATTGAGAGTGTGGAGGAGGCACAGTGGCAGATAGACACAGCAATTTGTAAATGTTTGGAAGAGAGCAAACCAGTTTACATCAGCATTTGCTGCAATTTGGTCGACATTCCTCATCCTTCCTTCTCAATGCAACCACTCATCCCACTCTCCATCTCTCCCAAGGAAAGTAACCAGATGGGTTTGGAGGTGGCAGTGGCCCAGGCGGCGGAGCTGCTAAATAGCGCCGTAAAACCAGTGATGGTCGGCGGGAGGAAGCTGAGACCCGCCGGAGCCGGGGCGGCGTTCCTCGACCTCGCCGACGCTTGTGGCTATGCCGTCGCCGTTACGCCGTCGGCGAAAGGCCTGTTTCCGGAGACTCACCCTCACTTCATCGGAACTTACTGGGGAGCCATCAGCTCCGCCGTCTGCCGCGAAACGGTGGAAATAGCCGACGCCTCGCTCTTCGCCGGCCCCAATTTCGACGACGTCGAAACGCTCGGCTTCTCGCTTGCCTACAAGAAGAACAAGGCCATTATTGTAGAGCCCGAACGCGTTGTGTTCCCTAATGGGCCGCGTTTTGGGCCCATTTTAATGAAGGACTTCCTTTGGGCTTTGGCCAAGCAACTGAAGCCCAACCCAACTGCTTATGAGAATTATCGCCGGATTTATATTCCGGAGTCGGGTCACGTCCAGTCGGAGCCGGGCGAGGCCTTGCGGGTCAACGTTCTTTTCAAACATATCCGAAAGATGTTAATGAGTGACATGACTGTCATATCTGAAAGCGGTGACTCTTGGTTTCACTGTCAAAAATTGAAATTACCACAATCATGCGGGTACGAGGTGCAATTGCTATATGGATCAATTGGATGGTCTCTAGGTGCAACTCTTGGTTATGCACAAGCCGCACCAGAAAAGCGAGTGGTTCTTTTTATCGGTGATGGGAGCTTTCAGATGTGTCCACAAGATGTATCGACAATGTTAAGATTGGGACAAAGAAATATAATATTTTTGATAAATAATGGTGGATACACCCTTCAAGTAGAGTTTCACGATGGTCCTTATACTGTCATCAAGAATTGGGACTACACTCTTCTCGTTGATGCTATGAACAATCATGTTGGCAAATGTTGGACTACCAAGGTTGAAACGGAAGCAGAGTTAGTGCAAGCAATTGAAACATCAATGAGAGATCAAAATGATTGTTTGTGCTTCATAGAAGCTATTGTTCATAGAGATGATACAAGTAAAGAGTTACTTGAATTTGGGAACACCCTTGATGCTATCATTAGTCGTCCTCCCAAACTTTAG

Coding sequence (CDS)

ATGGCCACCGACGCATCGACGCTAGGCCACTATCTCGGCCGCCGCCTCGTTGAAATCGGCGTTTCCGATATTTTTGCCGTTCCGGGAGACTCCATTCTTTCACTACTCGACTATTTCGCGGCGGAGCAAGGCCTGAATCTCGTCGGCTGCTGCAGCGAGCTGAATGCCGGCTACGCGGCTGATGGCTACGCCAGGCGCCGCGGCGTCGGAGCCTGCGCTGTCACCTTCACCGTCGGCGGCCTCAGCCTGATCAACGCCGTCGCCGGAGCTTACTCCGAGGACCTTCCGGTGATTTGTATTGTGGGTGGGCCCAACTCCAATTATTATGGGACTAAAAACATTCTCCACCATACCATTGGATCCCCCGATTTCAGTCAAGAACTCCGCTGCTTCCGAAATTTTACCTGTTATCAGGCCATTATTGAGAGTGTGGAGGAGGCACAGTGGCAGATAGACACAGCAATTTGTAAATGTTTGGAAGAGAGCAAACCAGTTTACATCAGCATTTGCTGCAATTTGGTCGACATTCCTCATCCTTCCTTCTCAATGCAACCACTCATCCCACTCTCCATCTCTCCCAAGGAAAGTAACCAGATGGGTTTGGAGGTGGCAGTGGCCCAGGCGGCGGAGCTGCTAAATAGCGCCGTAAAACCAGTGATGGTCGGCGGGAGGAAGCTGAGACCCGCCGGAGCCGGGGCGGCGTTCCTCGACCTCGCCGACGCTTGTGGCTATGCCGTCGCCGTTACGCCGTCGGCGAAAGGCCTGTTTCCGGAGACTCACCCTCACTTCATCGGAACTTACTGGGGAGCCATCAGCTCCGCCGTCTGCCGCGAAACGGTGGAAATAGCCGACGCCTCGCTCTTCGCCGGCCCCAATTTCGACGACGTCGAAACGCTCGGCTTCTCGCTTGCCTACAAGAAGAACAAGGCCATTATTGTAGAGCCCGAACGCGTTGTGTTCCCTAATGGGCCGCGTTTTGGGCCCATTTTAATGAAGGACTTCCTTTGGGCTTTGGCCAAGCAACTGAAGCCCAACCCAACTGCTTATGAGAATTATCGCCGGATTTATATTCCGGAGTCGGGTCACGTCCAGTCGGAGCCGGGCGAGGCCTTGCGGGTCAACGTTCTTTTCAAACATATCCGAAAGATGTTAATGAGTGACATGACTGTCATATCTGAAAGCGGTGACTCTTGGTTTCACTGTCAAAAATTGAAATTACCACAATCATGCGGGTACGAGGTGCAATTGCTATATGGATCAATTGGATGGTCTCTAGGTGCAACTCTTGGTTATGCACAAGCCGCACCAGAAAAGCGAGTGGTTCTTTTTATCGGTGATGGGAGCTTTCAGATGTGTCCACAAGATGTATCGACAATGTTAAGATTGGGACAAAGAAATATAATATTTTTGATAAATAATGGTGGATACACCCTTCAAGTAGAGTTTCACGATGGTCCTTATACTGTCATCAAGAATTGGGACTACACTCTTCTCGTTGATGCTATGAACAATCATGTTGGCAAATGTTGGACTACCAAGGTTGAAACGGAAGCAGAGTTAGTGCAAGCAATTGAAACATCAATGAGAGATCAAAATGATTGTTTGTGCTTCATAGAAGCTATTGTTCATAGAGATGATACAAGTAAAGAGTTACTTGAATTTGGGAACACCCTTGATGCTATCATTAGTCGTCCTCCCAAACTTTAG

Protein sequence

MATDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSPDFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFSMQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADACGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFSLAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGHVQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEFHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIVHRDDTSKELLEFGNTLDAIISRPPKL
Homology
BLAST of Moc01g19860 vs. NCBI nr
Match: XP_022144022.1 (pyruvate decarboxylase 1-like [Momordica charantia])

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MATDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAA 60
           MATDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAA
Sbjct: 62  MATDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAA 121

Query: 61  DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIG 120
           DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIG
Sbjct: 122 DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIG 181

Query: 121 SPDFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPS 180
           SPDFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPS
Sbjct: 182 SPDFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPS 241

Query: 181 FSMQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLAD 240
           FSMQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLAD
Sbjct: 242 FSMQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLAD 301

Query: 241 ACGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLG 300
           ACGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLG
Sbjct: 302 ACGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLG 361

Query: 301 FSLAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPES 360
           FSLAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPES
Sbjct: 362 FSLAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPES 421

Query: 361 GHVQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGS 420
           GHVQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGS
Sbjct: 422 GHVQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGS 481

Query: 421 IGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQV 480
           IGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQV
Sbjct: 482 IGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQV 541

Query: 481 EFHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEA 540
           EFHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEA
Sbjct: 542 EFHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEA 601

Query: 541 IVHRDDTSKELLEFGNTLDAIISRPPKL 569
           IVHRDDTSKELLEFGNTLDAIISRPPKL
Sbjct: 602 IVHRDDTSKELLEFGNTLDAIISRPPKL 629

BLAST of Moc01g19860 vs. NCBI nr
Match: XP_022144001.1 (pyruvate decarboxylase 1-like [Momordica charantia])

HSP 1 Score: 968.0 bits (2501), Expect = 3.6e-278
Identity = 464/561 (82.71%), Postives = 510/561 (90.91%), Query Frame = 0

Query: 7   TLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYARR 66
           TLGHYL RRLVEIGV DIF+VPGD  L LLDYF AE GLNLVGCCSELNAGYAADGYARR
Sbjct: 44  TLGHYLARRLVEIGVGDIFSVPGDXNLLLLDYFVAEXGLNLVGCCSELNAGYAADGYARR 103

Query: 67  RGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSPDFSQ 126
           RGVGACAVTFTVGGLSLINAVAGAYSEDLPV+CIVGGPNSN YG+K I+HHTIG PDFSQ
Sbjct: 104 RGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDFSQ 163

Query: 127 ELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFSMQPL 186
           ELRCF+N TCYQAII++VEE QW+ID AICKCL E KPVYISICCNLV IPHPSFSM+ L
Sbjct: 164 ELRCFQNVTCYQAIIDNVEETQWEIDRAICKCLGERKPVYISICCNLVAIPHPSFSMERL 223

Query: 187 IPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADACGYAV 246
           IPLS+ PK+SNQMGLEVA  +AA+LLNSAVKPVMV G+KLRPA A AAF++LADACGYAV
Sbjct: 224 IPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRPAKAQAAFVELADACGYAV 283

Query: 247 AVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFSLAYK 306
           AVTPSAKGLFPE HPHFIGTYWGA+S+A C ETVEIADAS+FAG   D++ET+ FSL YK
Sbjct: 284 AVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLTYK 343

Query: 307 KNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGHVQSE 366
           KNKAIIVEPERVVFPNGP FGPILMKDFL ALA++LKPNPTAYENYRRIY+PESG +QSE
Sbjct: 344 KNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQSE 403

Query: 367 PGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIGWSLG 426
           PGEALRVNVLFKHI+KML S+MTVISE+GDSWFHCQKLKLP+SCGYEVQLLY SIGWSLG
Sbjct: 404 PGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWSLG 463

Query: 427 ATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEFHDGP 486
           ATLGYAQAAP++RVVL IGDGSFQM PQDVSTMLRLGQ+NIIFLINNGGYT++VE HDGP
Sbjct: 464 ATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHDGP 523

Query: 487 YTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIVHRDD 546
           Y VIKNWDYT +V+AM+N  GKCWT KV TE ELV+AIET+ +D+NDCLCFIEAI+HRDD
Sbjct: 524 YNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHRDD 583

Query: 547 TSKELLEFGNTLDAIISRPPK 568
           TSKELLEFG+ + AI  RPP+
Sbjct: 584 TSKELLEFGSKIAAISCRPPR 604

BLAST of Moc01g19860 vs. NCBI nr
Match: XP_022144024.1 (pyruvate decarboxylase 1-like [Momordica charantia])

HSP 1 Score: 962.2 bits (2486), Expect = 2.0e-276
Identity = 466/568 (82.04%), Postives = 509/568 (89.61%), Query Frame = 0

Query: 1   MATDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAA 60
           MAT AST+GHYLGRRLVEIGV DIF+VPGD  L LLDYF AEQGLNLVGCCSELNAGYAA
Sbjct: 1   MATAASTVGHYLGRRLVEIGVGDIFSVPGDFNLLLLDYFVAEQGLNLVGCCSELNAGYAA 60

Query: 61  DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIG 120
           DGYARRRGVGACAVTFTVGGLSLINAVAGAYSED+PV+CIVGGPNSN Y +K ILHHTIG
Sbjct: 61  DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDVPVVCIVGGPNSNDYWSKKILHHTIG 120

Query: 121 SPDFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPS 180
            PD  QELRCFRN TC+QAII+++EEAQW+ID AICKCLE+ KPVYISICC+LV IPHPS
Sbjct: 121 LPDIGQELRCFRNVTCHQAIIDNLEEAQWEIDRAICKCLEKRKPVYISICCDLVAIPHPS 180

Query: 181 FSMQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLAD 240
           FS+ PLIPLS S K SN+MGLEVAV +AA+LLN+AVKPV+VGG+KLR AGA AAF++LAD
Sbjct: 181 FSIHPLIPLSPSTKPSNKMGLEVAVEKAAQLLNNAVKPVLVGGKKLRAAGAEAAFVELAD 240

Query: 241 ACGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLG 300
           ACGYAVA+TPSAKGLFPE HPHFIGTYWGA+SSA C ETVEIADASLFAGPNF+DVET+ 
Sbjct: 241 ACGYAVALTPSAKGLFPENHPHFIGTYWGAVSSAFCGETVEIADASLFAGPNFNDVETVA 300

Query: 301 FSLAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPES 360
           FSLAYKKNKAIIVEP RVVFPNGP FGPILMKDFLWALAK+LKPNPTAYENYRR+YIPES
Sbjct: 301 FSLAYKKNKAIIVEPNRVVFPNGPSFGPILMKDFLWALAKRLKPNPTAYENYRRVYIPES 360

Query: 361 GHVQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGS 420
           G +QS+PGEALR NVLF+HI+KML  +MTVISE+GDSWF CQKLKLPQSCGYEVQL Y S
Sbjct: 361 GPLQSKPGEALRANVLFQHIQKMLTGNMTVISETGDSWFQCQKLKLPQSCGYEVQLQYAS 420

Query: 421 IGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQV 480
           IGWSLGATLGYAQAA ++RVVL IGDGSFQM PQDVSTMLRLGQRNIIFLINNGGYT++V
Sbjct: 421 IGWSLGATLGYAQAALDERVVLCIGDGSFQMSPQDVSTMLRLGQRNIIFLINNGGYTIEV 480

Query: 481 EFHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEA 540
           E HDGPY +IKNWDYT  V AM NH GKCWT KV+TE EL+ AIET++ DQ D LCFIEA
Sbjct: 481 ELHDGPYNIIKNWDYTAFVHAMRNHDGKCWTAKVQTEEELMIAIETAIEDQKDSLCFIEA 540

Query: 541 IVHRDDTSKELLEFGNTLDAIISRPPKL 569
           IVHRDDTSKELL FGN + A  S  PKL
Sbjct: 541 IVHRDDTSKELLGFGNRILAANSHSPKL 568

BLAST of Moc01g19860 vs. NCBI nr
Match: XP_004148631.3 (pyruvate decarboxylase 1 [Cucumis sativus] >KGN54392.1 hypothetical protein Csa_018074 [Cucumis sativus])

HSP 1 Score: 942.6 bits (2435), Expect = 1.6e-270
Identity = 448/566 (79.15%), Postives = 501/566 (88.52%), Query Frame = 0

Query: 3   TDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADG 62
           T  STLGHYL  RLVEIGVSDIF+VPGDS L L DYF AE+GLNLVGCC+ELNAGYAADG
Sbjct: 72  TAPSTLGHYLASRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGYAADG 131

Query: 63  YARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSP 122
           YAR RGVGACAVTFTVG LSLINA+AGAYSEDLPVICIVGGPNSN YG+K ILHHTIG P
Sbjct: 132 YARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 191

Query: 123 DFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFS 182
           DFSQELRCF+N TCYQAII+S+E+AQWQID AICKCLEESKPVYISICCNLV IPHPSFS
Sbjct: 192 DFSQELRCFQNVTCYQAIIDSLEDAQWQIDKAICKCLEESKPVYISICCNLVAIPHPSFS 251

Query: 183 MQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADAC 242
            QPLIPLS+SPK+SNQMGLE+AV +AA+LLN+A+KPVM+GG+KLRPA A AAFL+LAD+C
Sbjct: 252 AQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKAEAAFLELADSC 311

Query: 243 GYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFS 302
           GYAVAVTPSAKG+FPE HPHFIGTYWG +S+A C ETVEIADAS+F G N D++ET+G+S
Sbjct: 312 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGANLDELETVGYS 371

Query: 303 LAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGH 362
           LAYKKNKAIIV+P+ VVFPNG  +G + MKDFLWAL K+LKPN  AYENYRRIYI ES  
Sbjct: 372 LAYKKNKAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNSRAYENYRRIYIAESSP 431

Query: 363 VQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIG 422
            +SE GE LRVNVLFKHI+KML S+MTVI+E+GDSWFH QKLKLP+SCGYEVQLLY SIG
Sbjct: 432 PESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCGYEVQLLYASIG 491

Query: 423 WSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEF 482
           WSLGATLGYAQAAP KR++L IGDGSFQM PQDVSTML+LGQ+NIIFLINNGGYT++VE 
Sbjct: 492 WSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLGQKNIIFLINNGGYTIEVEI 551

Query: 483 HDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIV 542
           HDGPY +I NWDYT  VDA+NNH   CWTTKV TE ELV AIE +M+D+NDCLCFIE I 
Sbjct: 552 HDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIEIAMKDRNDCLCFIEVIA 611

Query: 543 HRDDTSKELLEFGNTLDAIISRPPKL 569
           HRDDTSKELLEFG+ + A+ S PPKL
Sbjct: 612 HRDDTSKELLEFGSRIAAMGSHPPKL 637

BLAST of Moc01g19860 vs. NCBI nr
Match: XP_038881352.1 (pyruvate decarboxylase 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 939.1 bits (2426), Expect = 1.8e-269
Identity = 450/566 (79.51%), Postives = 500/566 (88.34%), Query Frame = 0

Query: 3   TDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADG 62
           T  STLGHYL RRLV+IG+SDIF+VPGDS L L DYF AE+GLNLVGCC+ELNAGYAADG
Sbjct: 58  TTPSTLGHYLARRLVQIGISDIFSVPGDSNLVLFDYFVAERGLNLVGCCNELNAGYAADG 117

Query: 63  YARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSP 122
           YARRRGVGACAVTFTVG LSLINA+AGAYSEDLPVICIVGGPNSN YG+K ILHHTIG P
Sbjct: 118 YARRRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 177

Query: 123 DFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFS 182
           DFSQELRCF+N TCYQAII+++E+AQWQID AI KCLEE KPVYISICCNLV IPHPSFS
Sbjct: 178 DFSQELRCFQNVTCYQAIIDNLEDAQWQIDRAISKCLEERKPVYISICCNLVAIPHPSFS 237

Query: 183 MQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADAC 242
            Q LIPLS+SPK+SNQM LEVAV +AA+LLNSA+KPVM+GG+KLRPA A  AF++LADAC
Sbjct: 238 AQQLIPLSLSPKKSNQMALEVAVEKAAQLLNSAIKPVMIGGKKLRPAKAQEAFMELADAC 297

Query: 243 GYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFS 302
            YAVAVTPSAKG+FPE HPHFIGTYWG +S+A C ETVEIADAS+FAG   D++ET+ FS
Sbjct: 298 RYAVAVTPSAKGMFPEYHPHFIGTYWGTVSTAYCGETVEIADASIFAGATLDELETVAFS 357

Query: 303 LAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGH 362
           L YKKNKAIIVE +RVVFPNGP FGPILMKDFL A+AK+L PNPTAYENYRRIYI ESG 
Sbjct: 358 LTYKKNKAIIVEADRVVFPNGPSFGPILMKDFLSAIAKRLNPNPTAYENYRRIYIAESGP 417

Query: 363 VQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIG 422
           +QS PGEALRVNVLFKHI+KML S++ VISE+GDSWF CQKLKLP+SCGYE+QLLY SIG
Sbjct: 418 LQSNPGEALRVNVLFKHIQKMLSSNIVVISETGDSWFQCQKLKLPKSCGYEIQLLYASIG 477

Query: 423 WSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEF 482
           WSLGATLGYAQAAPEKR+VL IGDGSFQM PQDVSTM+ L Q NIIFLINNGGYT++VE 
Sbjct: 478 WSLGATLGYAQAAPEKRMVLCIGDGSFQMTPQDVSTMITLKQNNIIFLINNGGYTIEVEI 537

Query: 483 HDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIV 542
           HDGPY VIKNWDYT +V+AM+N  G CWTTK+ TE ELV AIE +M DQ DCLCFIE I+
Sbjct: 538 HDGPYNVIKNWDYTAIVNAMHNRDGNCWTTKIHTEEELVNAIEKAMGDQKDCLCFIEVII 597

Query: 543 HRDDTSKELLEFGNTLDAIISRPPKL 569
           HRDDTSKELLEFG+   AI SRPP+L
Sbjct: 598 HRDDTSKELLEFGSKFAAISSRPPRL 623

BLAST of Moc01g19860 vs. ExPASy Swiss-Prot
Match: A2XFI3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=2)

HSP 1 Score: 790.8 bits (2041), Expect = 1.0e-227
Identity = 376/561 (67.02%), Postives = 454/561 (80.93%), Query Frame = 0

Query: 6   STLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYAR 65
           ++LG +L RRLV++GVSD+FAVPGD  L+LLD+  AE GL LVGCC+ELNAGYAADGYAR
Sbjct: 44  ASLGRHLARRLVQVGVSDVFAVPGDFNLTLLDHLIAEPGLRLVGCCNELNAGYAADGYAR 103

Query: 66  RRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSPDFS 125
            RGVGACAVTFTVGGLS++NA+AGAYSE+LPVICI GGPNSN YGT  ILHHTIG PDFS
Sbjct: 104 ARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIAGGPNSNDYGTNRILHHTIGLPDFS 163

Query: 126 QELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFSMQP 185
           QELRCF+  TC+QA++ ++E+A  QIDTAI   L ESKPVY+SI CNL  +PHP+FS  P
Sbjct: 164 QELRCFQTVTCHQAVVTNLEDAHEQIDTAIATALRESKPVYLSISCNLPGLPHPTFSRDP 223

Query: 186 LIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADACGYA 245
            +P  ++P+ SN+MGLE AV    E LN AVKPV+VGG KLR A AG AF+DL DA GYA
Sbjct: 224 -VPFFLAPRLSNKMGLEAAVEATVEFLNKAVKPVLVGGPKLRVAKAGKAFVDLVDASGYA 283

Query: 246 VAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFSLAY 305
            AV PSAKGL PETHPHFIGTYWGA+S+A C E VE ADA LFAGP F+D  ++G+S   
Sbjct: 284 YAVMPSAKGLVPETHPHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSFLL 343

Query: 306 KKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGHVQS 365
           KK+KAIIV+PERV+  NGP FG ++MK+FL  LAK++  N TAYENY+RI++PE   ++S
Sbjct: 344 KKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLES 403

Query: 366 EPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIGWSL 425
           EP E LRVNVLFKH++KML SD  VI+E+GDSWF+CQKLKLP+ CGYE Q+ YGSIGWS+
Sbjct: 404 EPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSV 463

Query: 426 GATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEFHDG 485
           GA LGYAQ A +KRV+  IGDGSFQ+  QDVSTM+R  Q +IIFLINNGGYT++VE HDG
Sbjct: 464 GALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDG 523

Query: 486 PYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIVHRD 545
           PY VIKNW+YT LVDA++N  GKCWT+KV+ E EL +AI  ++ ++ DCLCFIE I H+D
Sbjct: 524 PYNVIKNWNYTGLVDAIHNGEGKCWTSKVKCEEELTEAIGMALGEKKDCLCFIEVIAHKD 583

Query: 546 DTSKELLEFGNTLDAIISRPP 567
           DTSKELLE+G+ + A  SRPP
Sbjct: 584 DTSKELLEWGSRVSAANSRPP 603

BLAST of Moc01g19860 vs. ExPASy Swiss-Prot
Match: Q10MW3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 2.5e-226
Identity = 376/561 (67.02%), Postives = 453/561 (80.75%), Query Frame = 0

Query: 6   STLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYAR 65
           ++LG +L RRLV++GVSD+FAVPGD  L+LLD+  AE GL LVGCC+ELNAGYAADGYAR
Sbjct: 44  ASLGRHLARRLVQVGVSDVFAVPGDFNLTLLDHLIAEPGLRLVGCCNELNAGYAADGYAR 103

Query: 66  RRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSPDFS 125
            RGVGACAVTFTVGGLS++NA+AGAYSE+LPVICI GGPNSN YGT  ILHHTIG PDFS
Sbjct: 104 ARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIAGGPNSNDYGTNRILHHTIGLPDFS 163

Query: 126 QELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFSMQP 185
           QELRCF+  TC+QA++ ++E+A  QIDTAI   L ESKPVY+SI CNL  +PHP+FS  P
Sbjct: 164 QELRCFQTVTCHQAVVTNLEDAHEQIDTAIATALRESKPVYLSISCNLPGLPHPTFSRDP 223

Query: 186 LIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADACGYA 245
            +P  ++P+ SN+MGLE AV    E LN AVKPV+VGG KLR A AG AF+DL DA GYA
Sbjct: 224 -VPFFLAPRLSNKMGLEAAVEATVEFLNKAVKPVLVGGPKLRVAKAGKAFVDLVDASGYA 283

Query: 246 VAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFSLAY 305
            AV PSAKGL PETHPHFIGTYWGA+S+A C E VE ADA LFAGP F+D  ++G+S   
Sbjct: 284 YAVMPSAKGLVPETHPHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSFLL 343

Query: 306 KKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGHVQS 365
           KK+KAIIV+PERV+  NGP FG ++MK+FL  LAK++  N TAYENY+RI++PE   ++S
Sbjct: 344 KKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLES 403

Query: 366 EPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIGWSL 425
           EP E LRVNVLFKH++KML SD  VI+E+GDSWF+CQKLKLP+ CGYE Q+ YGSIGWS+
Sbjct: 404 EPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSV 463

Query: 426 GATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEFHDG 485
           GA LGYAQ A +KRV+  IGDGSFQ+  QDVSTM+R  Q +IIFLINNGGYT++VE HDG
Sbjct: 464 GALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTIEVEIHDG 523

Query: 486 PYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIVHRD 545
           PY VIKNW+YT LVDA++N  GKCWT+KV+ E EL +AI  ++  + DCLCFIE I H+D
Sbjct: 524 PYNVIKNWNYTGLVDAIHNGEGKCWTSKVKCEEELTEAIGMAL-GEKDCLCFIEVIAHKD 583

Query: 546 DTSKELLEFGNTLDAIISRPP 567
           DTSKELLE+G+ + A  SRPP
Sbjct: 584 DTSKELLEWGSRVSAANSRPP 602

BLAST of Moc01g19860 vs. ExPASy Swiss-Prot
Match: Q9M040 (Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 6.2e-225
Identity = 374/564 (66.31%), Postives = 448/564 (79.43%), Query Frame = 0

Query: 3   TDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADG 62
           T  STLG +L RRLV+ GV+D+F+VPGD  L+LLD+  AE  LN +GCC+ELNAGYAADG
Sbjct: 38  TSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGYAADG 97

Query: 63  YARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSP 122
           YAR RGVGAC VTFTVGGLS++NA+AGAYSE+LPVICIVGGPNSN +GT  ILHHTIG P
Sbjct: 98  YARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLP 157

Query: 123 DFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFS 182
           DFSQELRCF+  TCYQA++ ++E+A  QID AI   L+ESKPVYISI CNL   PHP+F+
Sbjct: 158 DFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVYISISCNLAATPHPTFA 217

Query: 183 MQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADAC 242
             P +P  ++P+ SN MGLE AV    E LN AVKPVMVGG KLR A A  AFL+LADA 
Sbjct: 218 RDP-VPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKASEAFLELADAS 277

Query: 243 GYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFS 302
           GY +AV PS KGL PE HPHFIGTYWGA+S+  C E VE ADA +FAGP F+D  ++G+S
Sbjct: 278 GYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYS 337

Query: 303 LAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGH 362
           L  KK KAIIV P+RVV  NGP FG +LM DF   LAK++K N TAYENY RI++PE   
Sbjct: 338 LLLKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNETAYENYERIFVPEGKP 397

Query: 363 VQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIG 422
           ++ +PGE LRVN +F+HI+KML S+  VI+E+GDSWF+CQKLKLP+ CGYE Q+ YGSIG
Sbjct: 398 LKCKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIG 457

Query: 423 WSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEF 482
           WS+GATLGYAQA PEKRV+ FIGDGSFQ+  QD+STM+R GQ+ IIFLINNGGYT++VE 
Sbjct: 458 WSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTIEVEI 517

Query: 483 HDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIV 542
           HDGPY VIKNW+YT LVDA++N  GKCWTTKV  E ELV+AI+T+  ++ D LCFIE IV
Sbjct: 518 HDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFIEVIV 577

Query: 543 HRDDTSKELLEFGNTLDAIISRPP 567
           H+DDTSKELLE+G+ + A   RPP
Sbjct: 578 HKDDTSKELLEWGSRVSAANGRPP 600

BLAST of Moc01g19860 vs. ExPASy Swiss-Prot
Match: A2Y5L9 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 2.4e-224
Identity = 375/561 (66.84%), Postives = 449/561 (80.04%), Query Frame = 0

Query: 6   STLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYAR 65
           +TLG +L RRLV+IG +D+FAVPGD  L+LLDY  AE GL L+GCC+ELNAGYAADGYAR
Sbjct: 43  ATLGRHLARRLVQIGATDVFAVPGDFNLTLLDYLIAEPGLKLIGCCNELNAGYAADGYAR 102

Query: 66  RRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSPDFS 125
            RGVGACAVTFTVGGLS++NA+AGAYSE+LPVICIVGGPNSN YGT  ILHHTIG PDFS
Sbjct: 103 ARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFS 162

Query: 126 QELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFSMQP 185
           QELRCF+  TCYQA+I ++++A  QIDTAI   L ESKPVYIS+ CNL  + HP+FS +P
Sbjct: 163 QELRCFQTITCYQAVINNLDDAHEQIDTAIATALRESKPVYISVGCNLAGLSHPTFSREP 222

Query: 186 LIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADACGYA 245
            +PL ISP+ SN+  LE AV  AA+ LN AVKPVMVGG K+R A A  AF  +A++ GY 
Sbjct: 223 -VPLFISPRLSNKANLEYAVEAAADFLNKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYP 282

Query: 246 VAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFSLAY 305
           +AV PSAKGL PE HP FIGTYWGA+S+  C E VE ADA LFAGP F+D  ++G+SL  
Sbjct: 283 IAVMPSAKGLVPEHHPRFIGTYWGAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLL 342

Query: 306 KKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGHVQS 365
           K+ KA+IV+P+RVV  NGP FG ILM +FL ALAK+L  N TAY+NYRRI+IP+      
Sbjct: 343 KREKAVIVQPDRVVVGNGPAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNG 402

Query: 366 EPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIGWSL 425
           +P E LRVN+LFKHI++ML  D  VI+E+GDSWF+CQKL+LP+ CGYE Q+ YGSIGWS+
Sbjct: 403 QPDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSV 462

Query: 426 GATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEFHDG 485
           GATLGYAQAA +KRV+  IGDGSFQM  QDVSTMLR GQ++IIFLINNGGYT++VE HDG
Sbjct: 463 GATLGYAQAAKDKRVISCIGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDG 522

Query: 486 PYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIVHRD 545
           PY VIKNWDYT L+DA++N  G CWT KV TE EL++AI T+   + DCLCFIE IVH+D
Sbjct: 523 PYNVIKNWDYTGLIDAIHNSDGNCWTKKVRTEEELIEAIATATGAKKDCLCFIEIIVHKD 582

Query: 546 DTSKELLEFGNTLDAIISRPP 567
           DTSKELLE+G+ + A  SRPP
Sbjct: 583 DTSKELLEWGSRVSAANSRPP 602

BLAST of Moc01g19860 vs. ExPASy Swiss-Prot
Match: Q0DHF6 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 6.8e-224
Identity = 375/561 (66.84%), Postives = 448/561 (79.86%), Query Frame = 0

Query: 6   STLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYAR 65
           +TLG +L RRLV+IG +D+FAVPGD  L+LLDY  AE GL L+GCC+ELNAGYAADGYAR
Sbjct: 43  ATLGRHLARRLVQIGATDVFAVPGDFNLTLLDYLIAEPGLKLIGCCNELNAGYAADGYAR 102

Query: 66  RRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSPDFS 125
            RGVGACAVTFTVGGLS++NA+AGAYSE+LPVICIVGGPNSN YGT  ILHHTIG PDFS
Sbjct: 103 ARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFS 162

Query: 126 QELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFSMQP 185
           QELRCF+  TCYQA+I ++++A  QIDTAI   L ESKPVYIS+ CNL  + HP+FS +P
Sbjct: 163 QELRCFQTITCYQAVINNLDDAHEQIDTAIATALRESKPVYISVGCNLAGLSHPTFSREP 222

Query: 186 LIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADACGYA 245
            +PL ISP+ SN+  LE AV  AA+ LN AVKPVMVGG K+R A A  AF  +A++ GY 
Sbjct: 223 -VPLFISPRLSNKANLEYAVEAAADFLNKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYP 282

Query: 246 VAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFSLAY 305
            AV PSAKGL PE HP FIGTYWGA+S+  C E VE ADA LFAGP F+D  ++G+SL  
Sbjct: 283 FAVMPSAKGLVPEHHPRFIGTYWGAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLL 342

Query: 306 KKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGHVQS 365
           K+ KA+IV+P+RVV  NGP FG ILM +FL ALAK+L  N TAY+NYRRI+IP+      
Sbjct: 343 KREKAVIVQPDRVVVGNGPAFGCILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNG 402

Query: 366 EPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIGWSL 425
           +P E LRVN+LFKHI++ML  D  VI+E+GDSWF+CQKL+LP+ CGYE Q+ YGSIGWS+
Sbjct: 403 QPDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSV 462

Query: 426 GATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEFHDG 485
           GATLGYAQAA +KRV+  IGDGSFQM  QDVSTMLR GQ++IIFLINNGGYT++VE HDG
Sbjct: 463 GATLGYAQAAKDKRVISCIGDGSFQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDG 522

Query: 486 PYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIVHRD 545
           PY VIKNWDYT L+DA++N  G CWT KV TE EL++AI T+   + DCLCFIE IVH+D
Sbjct: 523 PYNVIKNWDYTGLIDAIHNSDGNCWTKKVRTEEELIEAIATATGAKKDCLCFIEIIVHKD 582

Query: 546 DTSKELLEFGNTLDAIISRPP 567
           DTSKELLE+G+ + A  SRPP
Sbjct: 583 DTSKELLEWGSRVSAANSRPP 602

BLAST of Moc01g19860 vs. ExPASy TrEMBL
Match: A0A6J1CSI1 (Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013814 PE=3 SV=1)

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0

Query: 1   MATDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAA 60
           MATDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAA
Sbjct: 62  MATDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAA 121

Query: 61  DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIG 120
           DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIG
Sbjct: 122 DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIG 181

Query: 121 SPDFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPS 180
           SPDFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPS
Sbjct: 182 SPDFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPS 241

Query: 181 FSMQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLAD 240
           FSMQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLAD
Sbjct: 242 FSMQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLAD 301

Query: 241 ACGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLG 300
           ACGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLG
Sbjct: 302 ACGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLG 361

Query: 301 FSLAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPES 360
           FSLAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPES
Sbjct: 362 FSLAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPES 421

Query: 361 GHVQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGS 420
           GHVQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGS
Sbjct: 422 GHVQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGS 481

Query: 421 IGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQV 480
           IGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQV
Sbjct: 482 IGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQV 541

Query: 481 EFHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEA 540
           EFHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEA
Sbjct: 542 EFHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEA 601

Query: 541 IVHRDDTSKELLEFGNTLDAIISRPPKL 569
           IVHRDDTSKELLEFGNTLDAIISRPPKL
Sbjct: 602 IVHRDDTSKELLEFGNTLDAIISRPPKL 629

BLAST of Moc01g19860 vs. ExPASy TrEMBL
Match: A0A6J1CS04 (Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013786 PE=3 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.7e-278
Identity = 464/561 (82.71%), Postives = 510/561 (90.91%), Query Frame = 0

Query: 7   TLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYARR 66
           TLGHYL RRLVEIGV DIF+VPGD  L LLDYF AE GLNLVGCCSELNAGYAADGYARR
Sbjct: 44  TLGHYLARRLVEIGVGDIFSVPGDXNLLLLDYFVAEXGLNLVGCCSELNAGYAADGYARR 103

Query: 67  RGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSPDFSQ 126
           RGVGACAVTFTVGGLSLINAVAGAYSEDLPV+CIVGGPNSN YG+K I+HHTIG PDFSQ
Sbjct: 104 RGVGACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDFSQ 163

Query: 127 ELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFSMQPL 186
           ELRCF+N TCYQAII++VEE QW+ID AICKCL E KPVYISICCNLV IPHPSFSM+ L
Sbjct: 164 ELRCFQNVTCYQAIIDNVEETQWEIDRAICKCLGERKPVYISICCNLVAIPHPSFSMERL 223

Query: 187 IPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADACGYAV 246
           IPLS+ PK+SNQMGLEVA  +AA+LLNSAVKPVMV G+KLRPA A AAF++LADACGYAV
Sbjct: 224 IPLSLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRPAKAQAAFVELADACGYAV 283

Query: 247 AVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFSLAYK 306
           AVTPSAKGLFPE HPHFIGTYWGA+S+A C ETVEIADAS+FAG   D++ET+ FSL YK
Sbjct: 284 AVTPSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLTYK 343

Query: 307 KNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGHVQSE 366
           KNKAIIVEPERVVFPNGP FGPILMKDFL ALA++LKPNPTAYENYRRIY+PESG +QSE
Sbjct: 344 KNKAIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQSE 403

Query: 367 PGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIGWSLG 426
           PGEALRVNVLFKHI+KML S+MTVISE+GDSWFHCQKLKLP+SCGYEVQLLY SIGWSLG
Sbjct: 404 PGEALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWSLG 463

Query: 427 ATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEFHDGP 486
           ATLGYAQAAP++RVVL IGDGSFQM PQDVSTMLRLGQ+NIIFLINNGGYT++VE HDGP
Sbjct: 464 ATLGYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHDGP 523

Query: 487 YTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIVHRDD 546
           Y VIKNWDYT +V+AM+N  GKCWT KV TE ELV+AIET+ +D+NDCLCFIEAI+HRDD
Sbjct: 524 YNVIKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHRDD 583

Query: 547 TSKELLEFGNTLDAIISRPPK 568
           TSKELLEFG+ + AI  RPP+
Sbjct: 584 TSKELLEFGSKIAAISCRPPR 604

BLAST of Moc01g19860 vs. ExPASy TrEMBL
Match: A0A6J1CR30 (Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013816 PE=3 SV=1)

HSP 1 Score: 962.2 bits (2486), Expect = 9.5e-277
Identity = 466/568 (82.04%), Postives = 509/568 (89.61%), Query Frame = 0

Query: 1   MATDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAA 60
           MAT AST+GHYLGRRLVEIGV DIF+VPGD  L LLDYF AEQGLNLVGCCSELNAGYAA
Sbjct: 1   MATAASTVGHYLGRRLVEIGVGDIFSVPGDFNLLLLDYFVAEQGLNLVGCCSELNAGYAA 60

Query: 61  DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIG 120
           DGYARRRGVGACAVTFTVGGLSLINAVAGAYSED+PV+CIVGGPNSN Y +K ILHHTIG
Sbjct: 61  DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDVPVVCIVGGPNSNDYWSKKILHHTIG 120

Query: 121 SPDFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPS 180
            PD  QELRCFRN TC+QAII+++EEAQW+ID AICKCLE+ KPVYISICC+LV IPHPS
Sbjct: 121 LPDIGQELRCFRNVTCHQAIIDNLEEAQWEIDRAICKCLEKRKPVYISICCDLVAIPHPS 180

Query: 181 FSMQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLAD 240
           FS+ PLIPLS S K SN+MGLEVAV +AA+LLN+AVKPV+VGG+KLR AGA AAF++LAD
Sbjct: 181 FSIHPLIPLSPSTKPSNKMGLEVAVEKAAQLLNNAVKPVLVGGKKLRAAGAEAAFVELAD 240

Query: 241 ACGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLG 300
           ACGYAVA+TPSAKGLFPE HPHFIGTYWGA+SSA C ETVEIADASLFAGPNF+DVET+ 
Sbjct: 241 ACGYAVALTPSAKGLFPENHPHFIGTYWGAVSSAFCGETVEIADASLFAGPNFNDVETVA 300

Query: 301 FSLAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPES 360
           FSLAYKKNKAIIVEP RVVFPNGP FGPILMKDFLWALAK+LKPNPTAYENYRR+YIPES
Sbjct: 301 FSLAYKKNKAIIVEPNRVVFPNGPSFGPILMKDFLWALAKRLKPNPTAYENYRRVYIPES 360

Query: 361 GHVQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGS 420
           G +QS+PGEALR NVLF+HI+KML  +MTVISE+GDSWF CQKLKLPQSCGYEVQL Y S
Sbjct: 361 GPLQSKPGEALRANVLFQHIQKMLTGNMTVISETGDSWFQCQKLKLPQSCGYEVQLQYAS 420

Query: 421 IGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQV 480
           IGWSLGATLGYAQAA ++RVVL IGDGSFQM PQDVSTMLRLGQRNIIFLINNGGYT++V
Sbjct: 421 IGWSLGATLGYAQAALDERVVLCIGDGSFQMSPQDVSTMLRLGQRNIIFLINNGGYTIEV 480

Query: 481 EFHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEA 540
           E HDGPY +IKNWDYT  V AM NH GKCWT KV+TE EL+ AIET++ DQ D LCFIEA
Sbjct: 481 ELHDGPYNIIKNWDYTAFVHAMRNHDGKCWTAKVQTEEELMIAIETAIEDQKDSLCFIEA 540

Query: 541 IVHRDDTSKELLEFGNTLDAIISRPPKL 569
           IVHRDDTSKELL FGN + A  S  PKL
Sbjct: 541 IVHRDDTSKELLGFGNRILAANSHSPKL 568

BLAST of Moc01g19860 vs. ExPASy TrEMBL
Match: A0A0A0L1C6 (Pyruvate decarboxylase OS=Cucumis sativus OX=3659 GN=Csa_4G312250 PE=3 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 7.8e-271
Identity = 448/566 (79.15%), Postives = 501/566 (88.52%), Query Frame = 0

Query: 3   TDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADG 62
           T  STLGHYL  RLVEIGVSDIF+VPGDS L L DYF AE+GLNLVGCC+ELNAGYAADG
Sbjct: 72  TAPSTLGHYLASRLVEIGVSDIFSVPGDSNLVLFDYFVAEKGLNLVGCCNELNAGYAADG 131

Query: 63  YARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSP 122
           YAR RGVGACAVTFTVG LSLINA+AGAYSEDLPVICIVGGPNSN YG+K ILHHTIG P
Sbjct: 132 YARSRGVGACAVTFTVGSLSLINAIAGAYSEDLPVICIVGGPNSNDYGSKKILHHTIGLP 191

Query: 123 DFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFS 182
           DFSQELRCF+N TCYQAII+S+E+AQWQID AICKCLEESKPVYISICCNLV IPHPSFS
Sbjct: 192 DFSQELRCFQNVTCYQAIIDSLEDAQWQIDKAICKCLEESKPVYISICCNLVAIPHPSFS 251

Query: 183 MQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADAC 242
            QPLIPLS+SPK+SNQMGLE+AV +AA+LLN+A+KPVM+GG+KLRPA A AAFL+LAD+C
Sbjct: 252 AQPLIPLSLSPKQSNQMGLEMAVEKAADLLNTAIKPVMIGGKKLRPAKAEAAFLELADSC 311

Query: 243 GYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFS 302
           GYAVAVTPSAKG+FPE HPHFIGTYWG +S+A C ETVEIADAS+F G N D++ET+G+S
Sbjct: 312 GYAVAVTPSAKGMFPENHPHFIGTYWGTVSTAFCGETVEIADASIFVGANLDELETVGYS 371

Query: 303 LAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGH 362
           LAYKKNKAIIV+P+ VVFPNG  +G + MKDFLWAL K+LKPN  AYENYRRIYI ES  
Sbjct: 372 LAYKKNKAIIVKPDSVVFPNGESYGAVQMKDFLWALGKRLKPNSRAYENYRRIYIAESSP 431

Query: 363 VQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIG 422
            +SE GE LRVNVLFKHI+KML S+MTVI+E+GDSWFH QKLKLP+SCGYEVQLLY SIG
Sbjct: 432 PESEAGEELRVNVLFKHIQKMLSSNMTVIAETGDSWFHSQKLKLPKSCGYEVQLLYASIG 491

Query: 423 WSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEF 482
           WSLGATLGYAQAAP KR++L IGDGSFQM PQDVSTML+LGQ+NIIFLINNGGYT++VE 
Sbjct: 492 WSLGATLGYAQAAPHKRLLLCIGDGSFQMAPQDVSTMLKLGQKNIIFLINNGGYTIEVEI 551

Query: 483 HDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIV 542
           HDGPY +I NWDYT  VDA+NNH   CWTTKV TE ELV AIE +M+D+NDCLCFIE I 
Sbjct: 552 HDGPYNIINNWDYTAFVDAVNNHQSNCWTTKVHTEEELVNAIEIAMKDRNDCLCFIEVIA 611

Query: 543 HRDDTSKELLEFGNTLDAIISRPPKL 569
           HRDDTSKELLEFG+ + A+ S PPKL
Sbjct: 612 HRDDTSKELLEFGSRIAAMGSHPPKL 637

BLAST of Moc01g19860 vs. ExPASy TrEMBL
Match: A0A5D3CG96 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G00020 PE=3 SV=1)

HSP 1 Score: 933.3 bits (2411), Expect = 4.7e-268
Identity = 450/566 (79.51%), Postives = 502/566 (88.69%), Query Frame = 0

Query: 6   STLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYAR 65
           STLGHYL RRLV+IG+SDIF+VPGDS L LLDYF AE+GLNLVGCCSELNAGYAADGYAR
Sbjct: 63  STLGHYLARRLVQIGISDIFSVPGDSNLILLDYFVAEKGLNLVGCCSELNAGYAADGYAR 122

Query: 66  RRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYG--TKNILHHTIGSPD 125
           RRGVGACAVTFTVGGLSLINA+AGAYSEDLPV+CIVGGPNSN YG   K ILHHTIG PD
Sbjct: 123 RRGVGACAVTFTVGGLSLINAIAGAYSEDLPVVCIVGGPNSNEYGKSNKKILHHTIGLPD 182

Query: 126 FSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFSM 185
           FSQEL+CFRN TCYQA+I ++++AQW+ID AI KCLEESKPVYISI CNL  IPHPSFSM
Sbjct: 183 FSQELQCFRNVTCYQAVINNLKDAQWEIDCAISKCLEESKPVYISIACNLAAIPHPSFSM 242

Query: 186 QPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADACG 245
           Q LIPLSISPK+SNQM LE+AV +AA+LLN AVKPV+VGG+KLR A A AAF++LA+ C 
Sbjct: 243 QQLIPLSISPKQSNQMSLEMAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANTCD 302

Query: 246 YAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFSL 305
           YAVAVTPSAKG+FPE HPHF+GTYWG +S+A C ETVEIADAS+F G  FD++ET+GFSL
Sbjct: 303 YAVAVTPSAKGMFPENHPHFMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGFSL 362

Query: 306 AYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGHV 365
           +YKKNKAIIVE + VVFPNGPRFGPILMKDFLWALAK++KPN TAYENYRRIYIPES   
Sbjct: 363 SYKKNKAIIVETDHVVFPNGPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESSPA 422

Query: 366 QSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIGW 425
            SEPGEALRVNVLFKHI+KML +DMTVISE+GDSWFH QKL+LPQSCGYE+Q++Y SIGW
Sbjct: 423 TSEPGEALRVNVLFKHIQKMLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASIGW 482

Query: 426 SLGATLGYAQAAPEK--RVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVE 485
           SLGATLGYAQA+     RVVL IGDGSFQM PQD+STMLRLGQRNIIFLINNGGYT++VE
Sbjct: 483 SLGATLGYAQASSSSNDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVE 542

Query: 486 FHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAI 545
            HDGPY VIKNWDY+ LVDAM+NH G CWTTKV+TE ELV+AIE + RD NDCLCFIE I
Sbjct: 543 IHDGPYNVIKNWDYSALVDAMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIEVI 602

Query: 546 VHRDDTSKELLEFGNTLDAIISRPPK 568
           VHRDDTSKELLEFGN L  + SRPP+
Sbjct: 603 VHRDDTSKELLEFGNKLAEMNSRPPE 628

BLAST of Moc01g19860 vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 781.6 bits (2017), Expect = 4.4e-226
Identity = 374/564 (66.31%), Postives = 448/564 (79.43%), Query Frame = 0

Query: 3   TDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADG 62
           T  STLG +L RRLV+ GV+D+F+VPGD  L+LLD+  AE  LN +GCC+ELNAGYAADG
Sbjct: 38  TSESTLGRHLSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGYAADG 97

Query: 63  YARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSP 122
           YAR RGVGAC VTFTVGGLS++NA+AGAYSE+LPVICIVGGPNSN +GT  ILHHTIG P
Sbjct: 98  YARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLP 157

Query: 123 DFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFS 182
           DFSQELRCF+  TCYQA++ ++E+A  QID AI   L+ESKPVYISI CNL   PHP+F+
Sbjct: 158 DFSQELRCFQTVTCYQAVVNNLEDAHEQIDKAIATALKESKPVYISISCNLAATPHPTFA 217

Query: 183 MQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADAC 242
             P +P  ++P+ SN MGLE AV    E LN AVKPVMVGG KLR A A  AFL+LADA 
Sbjct: 218 RDP-VPFDLTPRMSNTMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKASEAFLELADAS 277

Query: 243 GYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFS 302
           GY +AV PS KGL PE HPHFIGTYWGA+S+  C E VE ADA +FAGP F+D  ++G+S
Sbjct: 278 GYPLAVMPSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYS 337

Query: 303 LAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGH 362
           L  KK KAIIV P+RVV  NGP FG +LM DF   LAK++K N TAYENY RI++PE   
Sbjct: 338 LLLKKEKAIIVHPDRVVVANGPTFGCVLMSDFFRELAKRVKRNETAYENYERIFVPEGKP 397

Query: 363 VQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIG 422
           ++ +PGE LRVN +F+HI+KML S+  VI+E+GDSWF+CQKLKLP+ CGYE Q+ YGSIG
Sbjct: 398 LKCKPGEPLRVNAMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIG 457

Query: 423 WSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEF 482
           WS+GATLGYAQA PEKRV+ FIGDGSFQ+  QD+STM+R GQ+ IIFLINNGGYT++VE 
Sbjct: 458 WSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGGYTIEVEI 517

Query: 483 HDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIV 542
           HDGPY VIKNW+YT LVDA++N  GKCWTTKV  E ELV+AI+T+  ++ D LCFIE IV
Sbjct: 518 HDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAIKTATTEKKDSLCFIEVIV 577

Query: 543 HRDDTSKELLEFGNTLDAIISRPP 567
           H+DDTSKELLE+G+ + A   RPP
Sbjct: 578 HKDDTSKELLEWGSRVSAANGRPP 600

BLAST of Moc01g19860 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 773.5 bits (1996), Expect = 1.2e-223
Identity = 368/561 (65.60%), Postives = 449/561 (80.04%), Query Frame = 0

Query: 6   STLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYAR 65
           +TLG +L RRLV+ GV+D+F+VPGD  L+LLD+  AE  LNL+GCC+ELNAGYAADGYAR
Sbjct: 45  ATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYAR 104

Query: 66  RRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSPDFS 125
            RGVGAC VTFTVGGLS++NA+AGAYSE+LP+ICIVGGPNSN YGT  ILHHTIG PDFS
Sbjct: 105 SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFS 164

Query: 126 QELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFSMQP 185
           QELRCF+  TCYQA++ ++++A  QID AI   L+ESKPVYIS+ CNL  IPH +FS  P
Sbjct: 165 QELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFSRDP 224

Query: 186 LIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADACGYA 245
            +P S++P+ SN+MGLE AV    E LN AVKPVMVGG KLR A A  AF++LADA GYA
Sbjct: 225 -VPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYA 284

Query: 246 VAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFSLAY 305
           +A+ PSAKG  PE HPHFIGTYWGA+S+  C E VE ADA +FAGP F+D  ++G+SL  
Sbjct: 285 LAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLL 344

Query: 306 KKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGHVQS 365
           KK KAI+V+P+R+   NGP FG ILM DF   L+K++K N TAYENY RI++PE   ++ 
Sbjct: 345 KKEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKC 404

Query: 366 EPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIGWSL 425
           E  E LRVN +F+HI+KML S+  VI+E+GDSWF+CQKLKLP+ CGYE Q+ YGSIGWS+
Sbjct: 405 ESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSV 464

Query: 426 GATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEFHDG 485
           GATLGYAQA+PEKRV+ FIGDGSFQ+  QD+STMLR GQ+ IIFLINNGGYT++VE HDG
Sbjct: 465 GATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDG 524

Query: 486 PYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIVHRD 545
           PY VIKNW+YT LVDA++N  G CWT KV  E ELV+AI T+  ++ DCLCFIE I+H+D
Sbjct: 525 PYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKD 584

Query: 546 DTSKELLEFGNTLDAIISRPP 567
           DTSKELLE+G+ + A  SRPP
Sbjct: 585 DTSKELLEWGSRVSAANSRPP 604

BLAST of Moc01g19860 vs. TAIR 10
Match: AT5G54960.1 (pyruvate decarboxylase-2 )

HSP 1 Score: 768.1 bits (1982), Expect = 5.0e-222
Identity = 369/561 (65.78%), Postives = 445/561 (79.32%), Query Frame = 0

Query: 6   STLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYAR 65
           +TLG YL RRLVEIGV+D+F+VPGD  L+LLD+  AE  L L+GCC+ELNAGYAADGYAR
Sbjct: 45  ATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEPNLKLIGCCNELNAGYAADGYAR 104

Query: 66  RRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIGSPDFS 125
            RGVGAC VTFTVGGLS++NA+AGAYSE+LP+ICIVGGPNSN YGT  ILHHTIG PDF+
Sbjct: 105 SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFT 164

Query: 126 QELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPSFSMQP 185
           QELRCF+  TC+QA+I ++EEA   IDTAI   L+ESKPVYISI CNL  IP P+FS  P
Sbjct: 165 QELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESKPVYISISCNLPAIPLPTFSRHP 224

Query: 186 LIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADACGYA 245
            +P  +  K SNQ+GL+ AV  AAE LN AVKPV+VGG K+R A A  AF++LADA GY 
Sbjct: 225 -VPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGGPKMRVAKAADAFVELADASGYG 284

Query: 246 VAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGFSLAY 305
           +AV PSAKG  PE H HFIGTYWGA+S+A C E VE ADA LFAGP F+D  ++G+SL  
Sbjct: 285 LAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLL 344

Query: 306 KKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPESGHVQS 365
           KK KAIIV+P+RV   NGP FG +LMKDFL  LAK++K N T+YENY RIY+PE   ++ 
Sbjct: 345 KKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIKHNNTSYENYHRIYVPEGKPLRD 404

Query: 366 EPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGSIGWSL 425
            P E+LRVNVLF+HI+ ML S+  V++E+GDSWF+CQKLKLP+ CGYE Q+ YGSIGWS+
Sbjct: 405 NPNESLRVNVLFQHIQNMLSSESAVLAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSV 464

Query: 426 GATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQVEFHDG 485
           GATLGYAQA P +RV+  IGDGSFQ+  QDVSTM+R GQ+ IIFLINNGGYT++VE HDG
Sbjct: 465 GATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIRCGQKTIIFLINNGGYTIEVEIHDG 524

Query: 486 PYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEAIVHRD 545
           PY VIKNW+YT  V+A++N  GKCWT KV  E ELV+AI T+  ++ +  CFIE IVH+D
Sbjct: 525 PYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKAINTATNEEKESFCFIEVIVHKD 584

Query: 546 DTSKELLEFGNTLDAIISRPP 567
           DTSKELLE+G+ + A  SRPP
Sbjct: 585 DTSKELLEWGSRVSAANSRPP 604

BLAST of Moc01g19860 vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 765.8 bits (1976), Expect = 2.5e-221
Identity = 369/566 (65.19%), Postives = 442/566 (78.09%), Query Frame = 0

Query: 1   MATDASTLGHYLGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAA 60
           + + A+TLG +L RRLV+ GV+DIF VPGD  LSLLD   A   LN +GCC+ELNAGYAA
Sbjct: 25  LGSSAATLGRHLSRRLVQAGVTDIFTVPGDFNLSLLDQLIANPELNNIGCCNELNAGYAA 84

Query: 61  DGYARRRGVGACAVTFTVGGLSLINAVAGAYSEDLPVICIVGGPNSNYYGTKNILHHTIG 120
           DGYAR RGVGAC VTFTVGGLS++NA+AGAYSE+LPVICIVGGPNSN +GT  ILHHTIG
Sbjct: 85  DGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIG 144

Query: 121 SPDFSQELRCFRNFTCYQAIIESVEEAQWQIDTAICKCLEESKPVYISICCNLVDIPHPS 180
            PDFSQELRCF+  TCYQA++  +E+A  QID AI   L ESKPVYISI CNL  IPHP+
Sbjct: 145 LPDFSQELRCFQTVTCYQAVVNHLEDAHEQIDKAIATALRESKPVYISISCNLAAIPHPT 204

Query: 181 FSMQPLIPLSISPKESNQMGLEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLAD 240
           F+  P +P  ++P+ SN+  LE AV    E LN AVKPVMVGG KLR A A  AF++LAD
Sbjct: 205 FASYP-VPFDLTPRLSNKDCLEAAVEATLEFLNKAVKPVMVGGPKLRVAKARDAFVELAD 264

Query: 241 ACGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLG 300
           A GY VAV PSAKG  PE HPHFIGTYWGA+S+  C E VE ADA +FAGP F+D  ++G
Sbjct: 265 ASGYPVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCSEIVESADAYIFAGPIFNDYSSVG 324

Query: 301 FSLAYKKNKAIIVEPERVVFPNGPRFGPILMKDFLWALAKQLKPNPTAYENYRRIYIPES 360
           +SL  KK KAIIV P+ VV  NGP FG + M +F   LAK++KPN TAYENY RI++PE 
Sbjct: 325 YSLLLKKEKAIIVHPDSVVVANGPTFGCVRMSEFFRELAKRVKPNKTAYENYHRIFVPEG 384

Query: 361 GHVQSEPGEALRVNVLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYEVQLLYGS 420
             ++ +P E LR+N +F+HI+KML ++  VI+E+GDSWF+CQKLKLP+ CGYE Q+ YGS
Sbjct: 385 KPLKCKPREPLRINAMFQHIQKMLSNETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGS 444

Query: 421 IGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRLGQRNIIFLINNGGYTLQV 480
           IGWS+GATLGYAQA PEKRV+ FIGDGSFQ+  QDVSTM+R GQ+ IIFLINNGGYT++V
Sbjct: 445 IGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDVSTMIRNGQKTIIFLINNGGYTIEV 504

Query: 481 EFHDGPYTVIKNWDYTLLVDAMNNHVGKCWTTKVETEAELVQAIETSMRDQNDCLCFIEA 540
           E HDGPY VIKNW+YT LVDA++N  GKCWTTKV  E ELV+AI T+  ++ D LCFIE 
Sbjct: 505 EIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAINTATLEKKDSLCFIEV 564

Query: 541 IVHRDDTSKELLEFGNTLDAIISRPP 567
           IVH+DDTSKELLE+G+ + A   RPP
Sbjct: 565 IVHKDDTSKELLEWGSRVSAANGRPP 589

BLAST of Moc01g19860 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 84.0 bits (206), Expect = 4.4e-16
Identity = 128/556 (23.02%), Postives = 219/556 (39.39%), Query Frame = 0

Query: 12  LGRRLVEIGVSDIFAVPGDSILSLLDYFAAEQGLNLVGCCSELNAGYAADGYARRRGVGA 71
           + + L  +GV+ +F V G  + SL    A   G+  +   +E +AGYAA  Y      G 
Sbjct: 18  VAKSLSHLGVTHMFGVVGIPVTSLASR-AMALGIRFIAFHNEQSAGYAASAYGYL--TGK 77

Query: 72  CAVTFTVGGLSLINAVAG---AYSEDLPVICIVGGPNSNYYGTKNILHHTIGSPDFSQEL 131
             +  TV G   ++ +AG   A+    P++ I G  +             +G  DF QEL
Sbjct: 78  PGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCD----------QRDVGRGDF-QEL 137

Query: 132 RCFRNFTCYQAIIESVEEAQWQIDTAICKCLEES---KP--VYISICCNLVDIPHPSFSM 191
                   +  + E  ++ + +I   + + L+ +   +P   Y+ I  +++         
Sbjct: 138 DQIEAVKAFSKLSEKAKDVR-EIPDCVSRVLDRAVSGRPGGCYLDIPTDVLRQKISESEA 197

Query: 192 QPLI-PLSISPKESNQMG-LEVAVAQAAELLNSAVKPVMVGGRKLRPAGAGAAFLDLADA 251
             L+  +  S KE    G L   +  A  LL  A +P++V G+    + A      L + 
Sbjct: 198 DKLVDEVERSRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEI 257

Query: 252 CGYAVAVTPSAKGLFPETHPHFIGTYWGAISSAVCRETVEIADASLFAGPNFDDVETLGF 311
            G     TP  KGL P+TH  F  T   +++   C       D +L  G   + +   G 
Sbjct: 258 TGIPFLPTPMGKGLLPDTH-EFSATAARSLAIGKC-------DVALVVGARLNWLLHFGE 317

Query: 312 SLAYKKN-KAIIVE-PERVVFPNGPRFGPI-LMKDFLWALAKQLKPNPTAYENYRRIYIP 371
           S  + K+ K I+V+  E  +    P  G +   K  +  L +++K +P         ++ 
Sbjct: 318 SPKWDKDVKFILVDVSEEEIELRKPHLGIVGDAKTVIGLLNREIKDDPFCL-GKSNSWVE 377

Query: 372 ESGHVQSEPGEALRVN-----VLFKHIRKMLMSDMTVISESGDSWFHCQKLKLPQSCGYE 431
                  E GE + +      V F  +  M +    +++  G S     +       G  
Sbjct: 378 SISKKAKENGEKMEIQLAKDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRS 437

Query: 432 VQL-----------LYGSIGWSLGATLGYAQAAPEKRVVLFIGDGSFQMCPQDVSTMLRL 491
           V +            +G++G  LG  +  A A+P++ VV   GD  F     +V T++R 
Sbjct: 438 VLVQKEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY 497

Query: 492 GQRNIIFLINNGGY----TLQVEFHDGPY-------TVIKNWDYTLLVDAMNNHVGKCWT 528
               +I + NNGG         E   GP+       + + N  Y  L++A     GK + 
Sbjct: 498 NLAVVIIVFNNGGVYGGDRRGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFG---GKGYI 544

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144022.10.0e+00100.00pyruvate decarboxylase 1-like [Momordica charantia][more]
XP_022144001.13.6e-27882.71pyruvate decarboxylase 1-like [Momordica charantia][more]
XP_022144024.12.0e-27682.04pyruvate decarboxylase 1-like [Momordica charantia][more]
XP_004148631.31.6e-27079.15pyruvate decarboxylase 1 [Cucumis sativus] >KGN54392.1 hypothetical protein Csa_... [more]
XP_038881352.11.8e-26979.51pyruvate decarboxylase 1-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A2XFI31.0e-22767.02Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=... [more]
Q10MW32.5e-22667.02Pyruvate decarboxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC2 PE=2 S... [more]
Q9M0406.2e-22566.31Pyruvate decarboxylase 4 OS=Arabidopsis thaliana OX=3702 GN=PDC4 PE=2 SV=1[more]
A2Y5L92.4e-22466.84Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=... [more]
Q0DHF66.8e-22466.84Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1CSI10.0e+00100.00Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013814 PE=3 SV=1[more]
A0A6J1CS041.7e-27882.71Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013786 PE=3 SV=1[more]
A0A6J1CR309.5e-27782.04Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013816 PE=3 SV=1[more]
A0A0A0L1C67.8e-27179.15Pyruvate decarboxylase OS=Cucumis sativus OX=3659 GN=Csa_4G312250 PE=3 SV=1[more]
A0A5D3CG964.7e-26879.51Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
Match NameE-valueIdentityDescription
AT5G01320.14.4e-22666.31Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT4G33070.11.2e-22365.60Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G54960.15.0e-22265.78pyruvate decarboxylase-2 [more]
AT5G01330.12.5e-22165.19pyruvate decarboxylase-3 [more]
AT5G17380.14.4e-1623.02Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePIRSFPIRSF036565Pyruvt_ip_decrbcoord: 1..561
e-value: 1.3E-184
score: 612.5
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePANTHERPTHR43452PYRUVATE DECARBOXYLASEcoord: 4..567
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 205..313
e-value: 1.1E-20
score: 73.8
NoneNo IPR availableGENE3D3.40.50.970coord: 3..180
e-value: 2.3E-57
score: 195.5
NoneNo IPR availableGENE3D3.40.50.970coord: 362..555
e-value: 1.5E-52
score: 179.7
NoneNo IPR availableGENE3D3.40.50.1220coord: 194..357
e-value: 1.8E-39
score: 136.9
NoneNo IPR availablePIRSRPIRSR036565-2PIRSR036565-2coord: 7..551
e-value: 7.6E-158
score: 523.9
NoneNo IPR availablePANTHERPTHR43452:SF25PYRUVATE DECARBOXYLASE 1coord: 4..567
NoneNo IPR availableCDDcd07038TPP_PYR_PDC_IPDC_likecoord: 11..171
e-value: 1.77606E-82
score: 252.801
NoneNo IPR availableCDDcd02005TPP_PDC_IPDCcoord: 370..552
e-value: 1.15364E-73
score: 230.884
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 415..537
e-value: 1.6E-16
score: 60.5
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 7..176
e-value: 5.0E-35
score: 120.6
IPR000399TPP-binding enzyme, conserved sitePROSITEPS00187TPP_ENZYMEScoord: 429..448
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 372..566
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 7..183
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 200..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc01g19860.1Moc01g19860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004737 pyruvate decarboxylase activity
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0016831 carboxy-lyase activity
molecular_function GO:0003824 catalytic activity