Sed0019519 (gene) Chayote v1

BLAST of Sed0019519 vs. NCBI nr
Match: XP_022997372.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 [Cucurbita maxima])

HSP 1 Score: 1296.2 bits (3353), Expect = 0.0e+00
Identity = 679/843 (80.55%), Postives = 753/843 (89.32%), Query Frame = 0

Query: 1   MAEEAMEK-KKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTII 60
           + E AMEK KKV RENK +RSHFDVLG+CCSSE+PL+ENILKPLEGIK I+VIVPTRT+I
Sbjct: 2   VGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLVENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLY 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+NGKG I KKKWPSPFAIASGLLLTASFLKY+Y
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQVNGKG-ISKKKWPSPFAIASGLLLTASFLKYVY 121

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLF 180
           HPLRWLA+AAVAAGIFPILLKAISA+RHLRID NILA++AVVGTI++NDYMEAGSIVFLF
Sbjct: 122 HPLRWLAIAAVAAGIFPILLKAISAVRHLRIDANILAIVAVVGTISMNDYMEAGSIVFLF 181

Query: 181 SIAEWLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPID 240
           SIAEWLE+ ASHKANAAMSSLMSLAPQKA IAE+GEVV  K+V+L ++LAVK GE IPID
Sbjct: 182 SIAEWLESRASHKANAAMSSLMSLAPQKAIIAETGEVVAVKEVELKSVLAVKGGEVIPID 241

Query: 241 GIVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAEL 300
           GIVVDGKC+VDEKTLTGE+FPVPK KDS+VWAGTINLNGYI+VQTTVVAEDC VAKMAE 
Sbjct: 242 GIVVDGKCDVDEKTLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 301

Query: 301 VEEAQNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKS+TQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSAC
Sbjct: 302 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 361

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFR 420
           PCALILSTPVAAFCALTKAAMAGVLIKG DHLEVLAK+KV+AFDKTGTITRGEF+V++F+
Sbjct: 362 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTNFQ 421

Query: 421 TLQDDISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGK 480
            L+DDI+ HTLLHWVSSIESKSSHPMAAAL+NHAKLLSIDIKPE VEEFENFPGEGV GK
Sbjct: 422 ALRDDINFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 481

Query: 481 INGNNIYIGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKE 540
           I+G +IYIGS KIAARAGC++   LD+E KQGQT+GY+F G    G+FGLSD+CRSG KE
Sbjct: 482 IDGKDIYIGSTKIAARAGCSSAMKLDEEMKQGQTLGYVFCGEMAVGAFGLSDSCRSGAKE 541

Query: 541 AIEEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKRE-GPT 600
           A+E++KS GIKTAMLTGDCSAAAM+AQQQL NA+D+IHSELLPK KAN+IK FKR+ G T
Sbjct: 542 AMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKV 660
           AMVGDGLNDT ALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSH KV
Sbjct: 602 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCK 720
           VQNVILSI TK AI+GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRG+DHKHG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKACSTKHGRCNGGDGRLSHRHN--HHHDNHRCNVVDHRSPSLENHDHKHSCSEKKDHG 780
           SSK CSTKHGRC+G + R SH H+  HHH +HRC+VVD +SPS +NH+    C +     
Sbjct: 722 SSKLCSTKHGRCDGSNTRPSHHHHHGHHHHSHRCHVVDDKSPSRDNHN--RDCGD----- 781

Query: 781 LHHLNEHKFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHS-HHVAIDIHESANCERV 839
                  KF HN SNQCEK P E+ENN N   KVD+ NCNCHS HHVAIDIHE  +CERV
Sbjct: 782 -------KFHHNDSNQCEKNPHEKENNGNCSTKVDEPNCNCHSHHHVAIDIHEGTDCERV 829

BLAST of Sed0019519 vs. NCBI nr
Match: XP_022996926.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1289.2 bits (3335), Expect = 0.0e+00
Identity = 679/847 (80.17%), Postives = 750/847 (88.55%), Query Frame = 0

Query: 1   MAEEAMEK-KKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTII 60
           + E AMEK KKV  ENK +RSHFDVLG+CCSSE+PLIENILKPLEGIK I+VIVPTRT+I
Sbjct: 2   VGEVAMEKNKKVRGENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLY 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+NGKG I KKKWPSPFAIASGLLLTASFLKY+Y
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQVNGKG-ISKKKWPSPFAIASGLLLTASFLKYVY 121

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLF 180
           HPLRWLA+AAVAAGIFPILLKAISA+RHLRID NILA++AVVGTI++NDYMEAGSIVFLF
Sbjct: 122 HPLRWLAIAAVAAGIFPILLKAISAVRHLRIDANILAIVAVVGTISMNDYMEAGSIVFLF 181

Query: 181 SIAEWLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPID 240
           SIAEWLE+ ASHKANAAMSSLMSLAPQKA IAE+GEVV  K+V+L ++LAVK GE IPID
Sbjct: 182 SIAEWLESRASHKANAAMSSLMSLAPQKAIIAETGEVVAVKEVELKSVLAVKGGEVIPID 241

Query: 241 GIVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAEL 300
           GIVVDGKC+VDEKTLTGE+FPVPK KDS+VWAGTINLNGYI+VQTTVVAEDC VAKMAE 
Sbjct: 242 GIVVDGKCDVDEKTLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 301

Query: 301 VEEAQNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKS+TQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSAC
Sbjct: 302 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 361

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFR 420
           PCALILSTPVAAFCALTKAAMAGVLIKG DHLEVLAK+KV+AFDKTGTITRGEF+V++F+
Sbjct: 362 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTNFQ 421

Query: 421 TLQDDISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGK 480
            L+DDIS HTLLHWVSSIESKSSHPMAAAL+NHAKLLSIDIKPE VEEFENFPGEGV GK
Sbjct: 422 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 481

Query: 481 INGNNIYIGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKE 540
           I+G +IYIGS KIAARAGC++   LD+E KQGQT+GY+F G    G+FGLSD+CRSG KE
Sbjct: 482 IDGKDIYIGSTKIAARAGCSSAMKLDEEMKQGQTLGYVFCGEMAVGAFGLSDSCRSGAKE 541

Query: 541 AIEEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKRE-GPT 600
           A+E++KS GIKTAMLTGDCSAAAM+AQQQL NA+D+IHSELLPK KAN+IK FKR+ G T
Sbjct: 542 AMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKV 660
           AMVGDGLNDT ALA ADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSH KV
Sbjct: 602 AMVGDGLNDTPALAIADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCK 720
           VQNVILSI TK AI+GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRG+DHKHG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKACSTKHGRCNGGDGRLS---HRHNHHHDNHRCNVVDHRSPSLENHDHKHSCSEKKDH 780
           SSK CS KHGRC+G + R S   H H+HHH +HRC+VVD +SPS ENH HKH C EKKDH
Sbjct: 722 SSKLCSMKHGRCDGSNTRPSHHHHHHDHHHHSHRCHVVDDKSPSRENHVHKH-CCEKKDH 781

Query: 781 GLHHLNEH------KFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHSHHVAIDIHES 837
            L    +H      KF HNHS           NN N  +KV + NCNCHSHHVAIDIHE 
Sbjct: 782 NLQLPQDHNGDCGDKFHHNHS-----------NNGNCSKKVGEPNCNCHSHHVAIDIHEG 835

BLAST of Sed0019519 vs. NCBI nr
Match: XP_022929619.1 (cadmium/zinc-transporting ATPase HMA2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1288.5 bits (3333), Expect = 0.0e+00
Identity = 674/840 (80.24%), Postives = 748/840 (89.05%), Query Frame = 0

Query: 1   MAEEAMEK-KKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTII 60
           + E AMEK KKV RENK +RSHFDVLG+CCSSE+PLIENILKPLEGIK I+VIVPTRT+I
Sbjct: 2   VGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLY 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+ GKG I KKKWPSPFAIASGLLLTASFLKY+Y
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQLKGKG-ISKKKWPSPFAIASGLLLTASFLKYVY 121

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISA+RHLRIDVNILA+IAVVG +++NDYMEAGSIVFLF
Sbjct: 122 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 181

Query: 181 SIAEWLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPID 240
           SIAEWLE+ ASHKANAAM SLMSLAPQKATIAE+GEVV+ K+V L ++LAVK GE IPID
Sbjct: 182 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 241

Query: 241 GIVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAEL 300
           GIVVDGKC+VDEK+LTGE+FPVPK KDS+VWAGTINLNGYI+VQTTVVAEDC VAKMAE 
Sbjct: 242 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 301

Query: 301 VEEAQNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKS+TQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSAC
Sbjct: 302 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 361

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFR 420
           PCALILSTPVAAFCALTKAAMAGVLIKG DHLEVLAK+KV+AFDKTGTITRGEF+V+DF+
Sbjct: 362 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 421

Query: 421 TLQDDISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGK 480
            L+DDIS HTLLHWVSSIESKSSHPMAAAL+NHAKLLS DIKPE VEEFENFPGEGV GK
Sbjct: 422 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGK 481

Query: 481 INGNNIYIGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKE 540
           I+G +IYIGS KIAARAGC++   L++E KQGQT+GY+F      GSFGLSD+CRSG KE
Sbjct: 482 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 541

Query: 541 AIEEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKRE-GPT 600
            +EE+KS GIKTAMLTGDCSAAAM+AQQQL NA+D+IHSELLPK KAN+IK FKR+ G T
Sbjct: 542 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKV 660
           AMVGDGLNDT ALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSH KV
Sbjct: 602 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCK 720
           VQNVILSI TK AI+GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRG+DHKHG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKACSTKHGRCNGGDGRLS--HRHNHHHDNHRCNVVDHRSPSLENHDHKHSCSEKKDHG 780
           SSK CSTKHGRC+G + R S  H H+HHH +HRC+VVD + PS +NH+    C +     
Sbjct: 722 SSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDNHN--GDCGD----- 781

Query: 781 LHHLNEHKFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHSHHVAIDIHESANCERVE 837
                  KF HN SNQCEK PLE++NN N  +KV + NCNCHSHHVAIDIH+  +CER+E
Sbjct: 782 -------KFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 826

BLAST of Sed0019519 vs. NCBI nr
Match: XP_022929621.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 668/839 (79.62%), Postives = 744/839 (88.68%), Query Frame = 0

Query: 1   MAEEAMEKKKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIV 60
           + E AMEK K   +NK +RSHFDVLG+CCSSE+PLIENILKPLEGIK I+VIVPTRT+IV
Sbjct: 2   VGEVAMEKNK---KNKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIV 61

Query: 61  VHDSLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLYH 120
           VHDSLL+SQLQIVKALNEARLEA VQ+ GKG I KKKWPSPFAIASGLLLTASFLKY+YH
Sbjct: 62  VHDSLLVSQLQIVKALNEARLEAKVQLKGKG-ISKKKWPSPFAIASGLLLTASFLKYVYH 121

Query: 121 PLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFS 180
           PLRWLAVAAVAAGIFPILLKAISA+RHLRIDVNILA+IAVVGT+++NDYMEAGSIVFLFS
Sbjct: 122 PLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEAGSIVFLFS 181

Query: 181 IAEWLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDG 240
           IAEWLE+ ASHKANAAM SLMSLAPQKATIAE+GEVV+ K+V L ++LAVK GE IPIDG
Sbjct: 182 IAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDG 241

Query: 241 IVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELV 300
           IVVDGKC+VDEK+LTGE+FPVPK KDS+VWAGTINLNGYI+VQTT VAEDC VAKMAE V
Sbjct: 242 IVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTAVAEDCVVAKMAEFV 301

Query: 301 EEAQNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACP 360
           EEAQNNKS+TQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSACP
Sbjct: 302 EEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACP 361

Query: 361 CALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRT 420
           CALILSTPVAAFCALTKAAMAGVLIKG DHLEVLAK+KV+AFDKTGTITRGEF+V+DF+ 
Sbjct: 362 CALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQA 421

Query: 421 LQDDISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKI 480
           L+DDIS HTLLHWVSSIESKSSHPMAAAL+NHAKLLS DIKPE VEEFENFPGEGV GKI
Sbjct: 422 LRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGKI 481

Query: 481 NGNNIYIGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEA 540
           +G +IYIGS KIAARAGC++   L++E K GQT+GY+F      GSFGLSD+CRSG KE 
Sbjct: 482 DGKDIYIGSTKIAARAGCSSAMKLEEEMKPGQTLGYVFCEEMAVGSFGLSDSCRSGAKEG 541

Query: 541 IEEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKRE-GPTA 600
           +EE+KS GIKTAMLTGDCSAAAM+AQQQL NA+D+IHSELLPK KAN+IK FKR+ G TA
Sbjct: 542 MEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGATA 601

Query: 601 MVGDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVV 660
           MVGDGLNDT ALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSH KVV
Sbjct: 602 MVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVV 661

Query: 661 QNVILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCKS 720
           QNVILSI TK AI+GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRG+DHKHG KCCKS
Sbjct: 662 QNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCKS 721

Query: 721 SKACSTKHGRCNGGDGRLS--HRHNHHHDNHRCNVVDHRSPSLENHDHKHSCSEKKDHGL 780
           SK CSTKHGRC+G + R S  H H+HHH +HRC+V+D +SPS +NH+    C +      
Sbjct: 722 SKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVIDDKSPSRDNHN--GDCGD------ 781

Query: 781 HHLNEHKFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHSHHVAIDIHESANCERVE 837
                 KF HN SNQCEK PLE++NN N  +KV + NCNCHSHHVAIDIH+  +CER+E
Sbjct: 782 ------KFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 822

BLAST of Sed0019519 vs. NCBI nr
Match: XP_038891185.1 (LOW QUALITY PROTEIN: putative inactive cadmium/zinc-transporting ATPase HMA3 [Benincasa hispida])

HSP 1 Score: 1275.0 bits (3298), Expect = 0.0e+00
Identity = 686/867 (79.12%), Postives = 755/867 (87.08%), Query Frame = 0

Query: 3   EEAMEK--KKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIV 62
           +EA+EK  KKV+RENKLQRS+FDVLG+CCSSE+PLIENILKPLEGIK I+VIVPTRT+IV
Sbjct: 4   QEALEKKNKKVVRENKLQRSYFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIV 63

Query: 63  VHDSLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLYH 122
           VHDSLLISQLQIVKALNEARLEANVQ+NGKG I KK+WPSPFAIASGLLLTASFLKY+YH
Sbjct: 64  VHDSLLISQLQIVKALNEARLEANVQLNGKG-ISKKRWPSPFAIASGLLLTASFLKYVYH 123

Query: 123 PLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFS 182
           PLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILA++AVVGTIA++DYMEAGSIVFLFS
Sbjct: 124 PLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAIVAVVGTIAMDDYMEAGSIVFLFS 183

Query: 183 IAEWLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDG 242
           IAEWLE+ ASHKANAA  +LMSLAPQK    +  ++VD+K+VKLN++LAVKAG  IPIDG
Sbjct: 184 IAEWLESRASHKANAAXCTLMSLAPQKTLXLKLEKIVDAKEVKLNSVLAVKAGRVIPIDG 243

Query: 243 IVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELV 302
           IV++GK EVDEKTLTG +FP  K KDS+VW GTINLNGYI+V+TT VAEDC VAKMAELV
Sbjct: 244 IVIEGKSEVDEKTLTGRNFPSSKHKDSLVWXGTINLNGYISVRTTAVAEDCVVAKMAELV 303

Query: 303 EEAQNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACP 362
           EEAQNNKS+TQRFIDECAKYYTPAV++ISACLAAIPAA+RVHNLSHW HLALVVLVSACP
Sbjct: 304 EEAQNNKSKTQRFIDECAKYYTPAVVIISACLAAIPAALRVHNLSHWLHLALVVLVSACP 363

Query: 363 CALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRT 422
           CALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEF+V+ F+ 
Sbjct: 364 CALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFVVTHFQA 423

Query: 423 LQDDISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKI 482
           L+DDIS HTLLHWVSSIESKSSHPMAAAL+NH KLLSID+KP+NVEEFENFPGEGV GKI
Sbjct: 424 LRDDISFHTLLHWVSSIESKSSHPMAAALVNHGKLLSIDLKPKNVEEFENFPGEGVRGKI 483

Query: 483 NGNNIYIGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEA 542
           +GN+IYIGSRKIA RAG T   S DDETKQ QT+GY+F G T+ GSFGLSD+CRSGVKEA
Sbjct: 484 DGNDIYIGSRKIAVRAGYTPDLSFDDETKQEQTLGYVFCGGTIIGSFGLSDSCRSGVKEA 543

Query: 543 IEEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKREGP-TA 602
           IEEIKSFGIKTAMLTGDC+AAAM+AQ+QLGNALDIIHSELLPK KANIIK FK +G   A
Sbjct: 544 IEEIKSFGIKTAMLTGDCNAAAMHAQEQLGNALDIIHSELLPKEKANIIKDFKEDGAIIA 603

Query: 603 MVGDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVV 662
           MVGDGLNDT AL TADIGISMGVSGSALATETGNVILMSNDMRKIPK IKLAKK H KVV
Sbjct: 604 MVGDGLNDTPALVTADIGISMGVSGSALATETGNVILMSNDMRKIPKTIKLAKKFHKKVV 663

Query: 663 QNVILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCKS 722
           QNVILSI TKTAI+GLA AGHPLVWAAVLADVGTCLLVILNSMLLLRG +HKHG+KCCKS
Sbjct: 664 QNVILSISTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGINHKHGEKCCKS 723

Query: 723 SKACSTKHG-RCNGGDGRLS---HRHNHHHDNHRCNVVDHRSPSLE-NHDHKH------- 782
           SK C TK+G RC+GG+ RLS   H H+HHH +HRC+V D +S S E NH HKH       
Sbjct: 724 SKPCLTKYGQRCDGGNTRLSSHHHDHHHHHHHHRCHVADDQSSSRENNHVHKHYKIELPQ 783

Query: 783 -------SCSEKKDH---------GLHHLNEHKFDHNHSNQCEKKPLEQENNANYLRKVD 839
                    ++KK+H          + H  EHKF +NHSNQCEK     E+N N  +KV 
Sbjct: 784 DHDRENSEVNQKKNHQCSDECEETNVAHEKEHKFYYNHSNQCEK-----ESNENSSKKVG 843

BLAST of Sed0019519 vs. ExPASy Swiss-Prot
Match: Q9SZW4 (Cadmium/zinc-transporting ATPase HMA2 OS=Arabidopsis thaliana OX=3702 GN=HMA2 PE=2 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 8.4e-279
Identity = 507/803 (63.14%), Postives = 631/803 (78.58%), Query Frame = 0

Query: 12  MRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIVVHDSLLISQLQ 71
           M   K+ +S+FDVLG+CC+SE+PLIENIL  ++G+K+ +VIVP+RT+IVVHD+L++SQ Q
Sbjct: 1   MASKKMTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQ 60

Query: 72  IVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLYHPLRWLAVAAVA 131
           IVKALN+A+LEANV++ G+    K KWPSPFA+ SG+LL  SF KYLY P RWLAVAAV 
Sbjct: 61  IVKALNQAQLEANVRVTGETNF-KNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVV 120

Query: 132 AGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFSIAEWLETLASH 191
           AGI+PIL KA++++   RID+NIL V+ V  TI + DY EA  +VFLF+IAEWL++ AS+
Sbjct: 121 AGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASY 180

Query: 192 KANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDGIVVDGKCEVDE 251
           KA+A M SLMSLAPQKA IAE+GE V+  ++K NT++AVKAGE IPIDG+VVDG CEVDE
Sbjct: 181 KASAVMQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDE 240

Query: 252 KTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELVEEAQNNKSRTQ 311
           KTLTGE+FPVPK KDS VWAGTINLNGYITV TT +AEDC VAKMA+LVEEAQN+K+ TQ
Sbjct: 241 KTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQ 300

Query: 312 RFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACPCALILSTPVAA 371
           RFID+C+KYYTPA+++IS C  AIP A++VHNL HW HLALVVLVSACPC LILSTPVA 
Sbjct: 301 RFIDKCSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVAT 360

Query: 372 FCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRTLQDDISLHTLL 431
           FCALTKAA +G+LIKG D+LE LAK+K++AFDKTGTITRGEF+V DF++L +DISL +LL
Sbjct: 361 FCALTKAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSLSEDISLQSLL 420

Query: 432 HWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKINGNNIYIGSRK 491
           +WVSS ESKSSHPMAAA++++A+ +S++ KPE VE+++NFPGEG++GKI+G  +YIG+++
Sbjct: 421 YWVSSTESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKR 480

Query: 492 IAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEAIEEIKSFGIKT 551
           IA+RAGC +VP +D +TK G+TIGY++ G TLAG F LSDACRSGV +A++E+KS GIK 
Sbjct: 481 IASRAGCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLGIKI 540

Query: 552 AMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKR-EGPTAMVGDGLNDTLA 611
           AMLTGD  AAAM+AQ+QLGNA+DI+ +ELLP+ K+ IIK  KR EGPTAMVGDGLND  A
Sbjct: 541 AMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMVGDGLNDAPA 600

Query: 612 LATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVVQNVILSIVTKT 671
           LATADIGISMGVSGSALATETGN+ILMSND+R+IP+AIKLAK++  KVV+NV++SI  K 
Sbjct: 601 LATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKG 660

Query: 672 AIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCKSSKACSTKHGRC 731
           AI+ LA AGHPL+WAAVLADVGTCLLVILNSMLLL    HK G KC + S + S      
Sbjct: 661 AILALAFAGHPLIWAAVLADVGTCLLVILNSMLLL-SDKHKTGNKCYRESSSSSVLIAEK 720

Query: 732 NGGDGRLSHRHNHHHDNHRCNVVDHRSPSLENHDHKH----SCSEKKDHGLHHLNEHKFD 791
             GD                   D  +  L     KH     C  K         +   D
Sbjct: 721 LEGDA----------------AGDMEAGLLPKISDKHCKPGCCGTKTQEKAMKPAKASSD 780

Query: 792 HNHSNQCEKKPLEQENNANYLRK 810
           H+HS  CE K   Q++N   ++K
Sbjct: 781 HSHSGCCETK---QKDNVTVVKK 782

BLAST of Sed0019519 vs. ExPASy Swiss-Prot
Match: O64474 (Putative cadmium/zinc-transporting ATPase HMA4 OS=Arabidopsis thaliana OX=3702 GN=HMA4 PE=1 SV=2)

HSP 1 Score: 959.5 bits (2479), Expect = 2.4e-278
Identity = 509/832 (61.18%), Postives = 655/832 (78.73%), Query Frame = 0

Query: 7   EKKKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIVVHDSLL 66
           EKKKV    KLQ+S+FDVLG+CC+SE+P+IENILK L+G+K+ +VIVP+RT+IVVHDSLL
Sbjct: 9   EKKKV---KKLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSLL 68

Query: 67  ISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLYHPLRWLA 126
           IS  QI KALNEARLEANV++NG+    K KWPSPFA+ SGLLL  SFLK++Y PLRWLA
Sbjct: 69  ISPFQIAKALNEARLEANVRVNGETSF-KNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLA 128

Query: 127 VAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFSIAEWLE 186
           VAAVAAGI+PIL KA ++I+  RID+NIL +I V+ T+A+ D+MEA ++VFLF+I++WLE
Sbjct: 129 VAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLE 188

Query: 187 TLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDGIVVDGK 246
           T AS+KA + M SLMSLAPQKA IAE+GE V+  +VK++T++AVKAGE IPIDGIVVDG 
Sbjct: 189 TRASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGN 248

Query: 247 CEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELVEEAQNN 306
           CEVDEKTLTGE+FPVPKQ+DS VWAGTINLNGYI V+TT +A DC VAKMA+LVEEAQ++
Sbjct: 249 CEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSS 308

Query: 307 KSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACPCALILS 366
           K+++QR ID+C++YYTPA++++SAC+A +P  M+VHNL HWFHLALVVLVS CPC LILS
Sbjct: 309 KTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILS 368

Query: 367 TPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRTLQDDIS 426
           TPVA FCALTKAA +G+LIK  D+L+ L+K+K++AFDKTGTITRGEF+V DF++L  DI+
Sbjct: 369 TPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRDIN 428

Query: 427 LHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKINGNNIY 486
           L +LL+WVSS+ESKSSHPMAA ++++AK +S++ +PE VE+++NFPGEG++GKI+GN+I+
Sbjct: 429 LRSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIF 488

Query: 487 IGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEAIEEIKS 546
           IG++KIA+RAGC+TVP ++ +TK G+T+GY++ G  LAG F LSDACRSGV +A+ E+KS
Sbjct: 489 IGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKS 548

Query: 547 FGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKREGPTAMVGDGLN 606
            GIKTAMLTGD  AAAM+AQ+QLGN LD++H +LLP+ K+ II+ FK+EGPTAMVGDG+N
Sbjct: 549 LGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKEGPTAMVGDGVN 608

Query: 607 DTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVVQNVILSI 666
           D  ALATADIGISMG+SGSALAT+TGN+ILMSND+R+IP+A+KLA+++  KVV+NV LSI
Sbjct: 609 DAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSI 668

Query: 667 VTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCKSSKACSTK 726
           + K  I+ LA AGHPL+WAAVL DVGTCLLVI NSMLLLR       KKC ++S   S  
Sbjct: 669 ILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKKCYRAS--TSKL 728

Query: 727 HGRCNGGD---------GRLSHRHNHHHDNHRC-------NVVDHRSPSLENHDHKH--S 786
           +GR   GD         G L+   N    +  C       NVV  +  S  + DH H   
Sbjct: 729 NGRKLEGDDDYVVDLEAGLLTKSGNGQCKSSCCGDKKNQENVVMMKPSSKTSSDHSHPGC 788

Query: 787 CSEKKDHGLHHLNEHKFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHSH 821
           C +KK+  +  L +          C +K  + E +      V  S+C   SH
Sbjct: 789 CGDKKEEKVKPLVK-------DGCCSEKTRKSEGD-----MVSLSSCKKSSH 822

BLAST of Sed0019519 vs. ExPASy Swiss-Prot
Match: P0CW78 (Cadmium/zinc-transporting ATPase HMA3 OS=Arabidopsis thaliana OX=3702 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 4.1e-257
Identity = 458/723 (63.35%), Postives = 581/723 (80.36%), Query Frame = 0

Query: 4   EAMEKKKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIVVHD 63
           E  E KK+     LQ S+FDV+G+CCSSE+ ++ N+L+ ++G+K+ +VIVP+RT+IVVHD
Sbjct: 3   EGEESKKM----NLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSRTVIVVHD 62

Query: 64  SLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLYHPLR 123
           + LIS LQIVKALN+ARLEA+V+  G+  + K +WPSPFAI SG+LL  SF KY Y PL 
Sbjct: 63  TFLISPLQIVKALNQARLEASVRPYGETSL-KSQWPSPFAIVSGVLLVLSFFKYFYSPLE 122

Query: 124 WLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFSIAE 183
           WLA+ AV AG+FPIL KA++++   R+D+N L +IAV+ T+ + D+ EA +IVFLFS+A+
Sbjct: 123 WLAIVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIVFLFSVAD 182

Query: 184 WLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDGIVV 243
           WLE+ A+HKA+  MSSLMSLAP+KA IA++G  VD  +V +NT+++VKAGE+IPIDG+VV
Sbjct: 183 WLESSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESIPIDGVVV 242

Query: 244 DGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELVEEA 303
           DG C+VDEKTLTGESFPV KQ++S V A TINLNGYI V+TT +A DC VAKM +LVEEA
Sbjct: 243 DGSCDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKMTKLVEEA 302

Query: 304 QNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACPCAL 363
           Q ++++TQRFID+C++YYTPAV+V +AC A IP  ++V +LSHWFHLALVVLVS CPC L
Sbjct: 303 QKSQTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLVSGCPCGL 362

Query: 364 ILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRTLQD 423
           ILSTPVA FCALTKAA +G LIK GD LE LAK+K++AFDKTGTIT+ EF+VSDFR+L  
Sbjct: 363 ILSTPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVSDFRSLSP 422

Query: 424 DISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKINGN 483
            I+LH LL+WVSSIE KSSHPMAAALI++A  +S++ KP+ VE F+NFPGEGV+G+I+G 
Sbjct: 423 SINLHKLLNWVSSIECKSSHPMAAALIDYAISVSVEPKPDIVENFQNFPGEGVYGRIDGQ 482

Query: 484 NIYIGSRKIAARAGCTT--VPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEAI 543
           +IYIG+++IA RAGC T  VP ++   K+G+TIGYI+ GA L GSF L D CR GV +A+
Sbjct: 483 DIYIGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGCRYGVAQAL 542

Query: 544 EEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKREGPTAMV 603
           +E+KS GI+TAMLTGD   AAM  Q+QL NALDI+HSELLP+ KA II  FK +GPT MV
Sbjct: 543 KELKSLGIQTAMLTGDNQDAAMSTQEQLENALDIVHSELLPQDKARIIDDFKIQGPTMMV 602

Query: 604 GDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVVQN 663
           GDGLND  ALA ADIGISMG+SGSALATETG++ILMSND+RKIPK ++LAK+SH KV++N
Sbjct: 603 GDGLNDAPALAKADIGISMGISGSALATETGDIILMSNDIRKIPKGMRLAKRSHKKVIEN 662

Query: 664 VILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCKSSK 723
           V+LS+  K AI+ L   G+PLVWAAVLAD GTCLLVILNSM+LLR  D +     C  S 
Sbjct: 663 VVLSVSIKGAIMVLGFVGYPLVWAAVLADAGTCLLVILNSMILLR--DEREAVSTCYRSS 718

Query: 724 ACS 725
             S
Sbjct: 723 TSS 718

BLAST of Sed0019519 vs. ExPASy Swiss-Prot
Match: A3BF39 (Cadmium/zinc-transporting ATPase HMA2 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA2 PE=1 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 7.4e-251
Identity = 473/833 (56.78%), Postives = 618/833 (74.19%), Query Frame = 0

Query: 16  KLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIVVHDSLLISQLQIVKA 75
           + Q+S+FDVLG+CC SE+PL+E +L+PLEG++ +TVIVP+RT+IVVHD   ISQ QIVKA
Sbjct: 7   RCQKSYFDVLGICCPSEVPLVEKLLQPLEGVQKVTVIVPSRTVIVVHDVDAISQSQIVKA 66

Query: 76  LNEARLEANVQINGKGLIR-KKKWPSPFAIASGLLLTASFLKYLYHPLRWLAVAAVAAGI 135
           LN+ARLEA+V+  G G  +   KWPSP+ +  GLLL  S  ++ +HPL+W A+ A AAG+
Sbjct: 67  LNQARLEASVRAYGNGSEKITNKWPSPYVLLCGLLLVVSLFEHFWHPLKWFALVAAAAGL 126

Query: 136 FPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFSIAEWLETLASHKAN 195
            PI+L++I+AIR L +DVNIL +IAV G IAL DY EAG IVFLF+ AEWLET ASHKA 
Sbjct: 127 PPIVLRSIAAIRRLTLDVNILMLIAVAGAIALKDYSEAGFIVFLFTTAEWLETRASHKAT 186

Query: 196 AAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDGIVVDGKCEVDEKTL 255
           A MS+LMS+APQKA +AE+GEVV ++DVK+NT++AVKAGE IPIDG+VVDG+ EVDE TL
Sbjct: 187 AGMSALMSMAPQKAILAETGEVVAARDVKVNTVIAVKAGEVIPIDGVVVDGRSEVDESTL 246

Query: 256 TGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELVEEAQNNKSRTQRFI 315
           TGESFPV KQ DS VWAGT+N++GYI V+TT +A++ AVAKMA LVEEAQN++S TQR I
Sbjct: 247 TGESFPVSKQPDSQVWAGTLNIDGYIAVRTTAMADNSAVAKMARLVEEAQNSRSSTQRLI 306

Query: 316 DECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCA 375
           D CAKYYTPAV+V++  +AAIPA  + HNL HWF LALV+LVSACPCAL+LSTP+A FCA
Sbjct: 307 DTCAKYYTPAVVVMAGSVAAIPAIAKAHNLKHWFQLALVLLVSACPCALVLSTPIATFCA 366

Query: 376 LTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRTLQDDISLHTLLHWV 435
           L +AA  G+LIKGGD LE LA +KV AFDKTGTITRGEF V +F+ + + +SL  LL+WV
Sbjct: 367 LLRAARTGLLIKGGDVLESLASIKVAAFDKTGTITRGEFSVEEFQPVGERVSLQQLLYWV 426

Query: 436 SSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKINGNNIYIGSRKIAA 495
           SS+ES+SSHPMA+ L+++A+  S++ K ENV EF+ +PGEG++G+I+G  IYIG+++I +
Sbjct: 427 SSVESRSSHPMASVLVDYAQSKSVEPKSENVSEFQIYPGEGIYGEIDGAGIYIGNKRILS 486

Query: 496 RAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEAIEEIKSFGIKTAML 555
           RA C TVP + D   +G TIGY+     L G F LSDACR+G  EAI+E++S GIK+ ML
Sbjct: 487 RASCETVPDMKD--MKGVTIGYVACNNELIGVFTLSDACRTGSAEAIKELRSLGIKSVML 546

Query: 556 TGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFK-REGPTAMVGDGLNDTLALAT 615
           TGD SAAA YAQ QLGN L  +H+ELLP+ K  I+   K ++GPT MVGDG+ND  ALA 
Sbjct: 547 TGDSSAAATYAQNQLGNILAEVHAELLPEDKVRIVGELKEKDGPTLMVGDGMNDAPALAK 606

Query: 616 ADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVVQNVILSIVTKTAII 675
           AD+G+SMGVSGSA+A ET +V LMSND+R+IPKA++LA+++H  ++ N+I S++TK AI+
Sbjct: 607 ADVGVSMGVSGSAVAMETSHVALMSNDIRRIPKAVRLARRTHRTIIVNIIFSVITKLAIV 666

Query: 676 GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCKS-----SKACSTKHG 735
           GLA AGHPL+WAAVLADVGTCLLVI+ SMLLLR  D +  KKC  S      K CS+ H 
Sbjct: 667 GLAFAGHPLIWAAVLADVGTCLLVIMYSMLLLREKDSRKAKKCAASHHGSPKKCCSSSH- 726

Query: 736 RCNGGDGRLSHRHNHHHDNHRC-NVVDHRSPSL-----ENHDHKHSCSEKKDHGLHHLNE 795
             +G   + +H  +HH  +  C ++V  +  S+      +H H+H+  E+  H  H  N+
Sbjct: 727 --HGSHAKKNHGVSHHCSDGPCKSMVSCKESSVAKNACHDHHHEHNHHEEPAH-KHSSNQ 786

Query: 796 HK-FDHNHSNQCEKKPLEQ--------ENNANY------LRKVDKSNCNCHSH 821
           H   DH+H +   K+P  Q         +  N+      L    K +C+ H H
Sbjct: 787 HGCHDHSHGHSNCKEPSNQLITNKHACHDGHNHCADTSNLHDTKKHDCHGHEH 833

BLAST of Sed0019519 vs. ExPASy Swiss-Prot
Match: Q8H384 (Cadmium/zinc-transporting ATPase HMA3 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 3.2e-209
Identity = 379/693 (54.69%), Postives = 513/693 (74.03%), Query Frame = 0

Query: 16  KLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIVVHDSLLISQLQIVKA 75
           K ++++ DVLG+CCS+E+ L+E +L PL+G++ ++V+V +RT++V HD     +  IVKA
Sbjct: 40  KRKKTYLDVLGVCCSAEVALVERLLAPLDGVRVVSVVVASRTVVVEHDPAAAPESAIVKA 99

Query: 76  LNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLYHPLRWLAVAAVAAGIF 135
           LN+A LEA+V+  G   +   +WPSP+ +ASG+LLTASF ++L+ PL+ LAVAAV AG  
Sbjct: 100 LNKAGLEASVRAYGSSGV-VSRWPSPYIVASGVLLTASFFEWLFPPLQCLAVAAVVAGAP 159

Query: 136 PILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFSIAEWLETLASHKANA 195
           P++ +  +A   L +D+N+L +IAV G + L DY EAG+IVFLF+ AEWLETLA  KA+A
Sbjct: 160 PMVRRGFAAASRLSLDINVLMLIAVAGALCLGDYTEAGAIVFLFTTAEWLETLACTKASA 219

Query: 196 AMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDGIVVDGKCEVDEKTLT 255
            MSSLM + P KA IA +GEVV  +DV++  ++AV+AGE +P+DG+VVDG+ EVDE++LT
Sbjct: 220 GMSSLMGMLPVKAVIATTGEVVSVRDVRVGDVVAVRAGEIVPVDGVVVDGQSEVDERSLT 279

Query: 256 GESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELVEEAQNNKSRTQRFID 315
           GESFPVPKQ  S VWAGT+N +GYI V+TT +AE+  VAKM  LVE AQN++S+TQR ID
Sbjct: 280 GESFPVPKQPHSEVWAGTMNFDGYIAVRTTALAENSTVAKMERLVEAAQNSRSKTQRLID 339

Query: 316 ECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCAL 375
            CAKYYTPAV+V++A +A IPA +    L  W+ LALV+LVSACPCAL+LSTPVA+FCA+
Sbjct: 340 SCAKYYTPAVVVVAAGVALIPALLGADGLEQWWKLALVMLVSACPCALVLSTPVASFCAM 399

Query: 376 TKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRTLQD-DISLHTLLHWV 435
            +AA  G+ IKGGD LE L +++ +AFDKTGTITRGEF +  F  + D  + +  LL+W+
Sbjct: 400 LRAARMGIFIKGGDVLESLGEIRAVAFDKTGTITRGEFSIDSFHLVGDHKVEMDHLLYWI 459

Query: 436 SSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKINGNNIYIGSRKIAA 495
           +SIESKSSHPMAAAL+ +A+  SI   PENV +F  +PGEG++G+I+G +IYIG+R+  A
Sbjct: 460 ASIESKSSHPMAAALVEYAQSKSIQPNPENVGDFRIYPGEGIYGEIHGKHIYIGNRRTLA 519

Query: 496 RAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEAIEEIKSFGIKTAML 555
           RA          E  +G +IGY+     LAG F LSD CR+G  EAI E+ S GIK+ ML
Sbjct: 520 RASSPQSTQEMGEMIKGVSIGYVICDGELAGVFSLSDDCRTGAAEAIRELGSLGIKSVML 579

Query: 556 TGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFK-REGPTAMVGDGLNDTLALAT 615
           TGD SAAA +AQ QLG  ++ +HSELLP+ K  ++ G K R GPT MVGDG+ND  ALA 
Sbjct: 580 TGDSSAAATHAQGQLGGVMEELHSELLPEDKVRLVSGLKARFGPTMMVGDGMNDAAALAA 639

Query: 616 ADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVVQNVILSIVTKTAII 675
           AD+G+SMG+SGSA A ET +  LMS+D+ ++P+A++L + +   +  NV  S+  K A++
Sbjct: 640 ADVGVSMGISGSAAAMETSHATLMSSDVLRVPEAVRLGRCARRTIAVNVAGSVAVKAAVL 699

Query: 676 GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLR 707
            LA A  P++WAAVLADVGTCLLV+LNSM LLR
Sbjct: 700 ALAAAWRPVLWAAVLADVGTCLLVVLNSMTLLR 731

BLAST of Sed0019519 vs. ExPASy TrEMBL
Match: A0A6J1K4T4 (putative inactive cadmium/zinc-transporting ATPase HMA3 OS=Cucurbita maxima OX=3661 GN=LOC111492308 PE=3 SV=1)

HSP 1 Score: 1296.2 bits (3353), Expect = 0.0e+00
Identity = 679/843 (80.55%), Postives = 753/843 (89.32%), Query Frame = 0

Query: 1   MAEEAMEK-KKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTII 60
           + E AMEK KKV RENK +RSHFDVLG+CCSSE+PL+ENILKPLEGIK I+VIVPTRT+I
Sbjct: 2   VGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLVENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLY 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+NGKG I KKKWPSPFAIASGLLLTASFLKY+Y
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQVNGKG-ISKKKWPSPFAIASGLLLTASFLKYVY 121

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLF 180
           HPLRWLA+AAVAAGIFPILLKAISA+RHLRID NILA++AVVGTI++NDYMEAGSIVFLF
Sbjct: 122 HPLRWLAIAAVAAGIFPILLKAISAVRHLRIDANILAIVAVVGTISMNDYMEAGSIVFLF 181

Query: 181 SIAEWLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPID 240
           SIAEWLE+ ASHKANAAMSSLMSLAPQKA IAE+GEVV  K+V+L ++LAVK GE IPID
Sbjct: 182 SIAEWLESRASHKANAAMSSLMSLAPQKAIIAETGEVVAVKEVELKSVLAVKGGEVIPID 241

Query: 241 GIVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAEL 300
           GIVVDGKC+VDEKTLTGE+FPVPK KDS+VWAGTINLNGYI+VQTTVVAEDC VAKMAE 
Sbjct: 242 GIVVDGKCDVDEKTLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 301

Query: 301 VEEAQNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKS+TQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSAC
Sbjct: 302 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 361

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFR 420
           PCALILSTPVAAFCALTKAAMAGVLIKG DHLEVLAK+KV+AFDKTGTITRGEF+V++F+
Sbjct: 362 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTNFQ 421

Query: 421 TLQDDISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGK 480
            L+DDI+ HTLLHWVSSIESKSSHPMAAAL+NHAKLLSIDIKPE VEEFENFPGEGV GK
Sbjct: 422 ALRDDINFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 481

Query: 481 INGNNIYIGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKE 540
           I+G +IYIGS KIAARAGC++   LD+E KQGQT+GY+F G    G+FGLSD+CRSG KE
Sbjct: 482 IDGKDIYIGSTKIAARAGCSSAMKLDEEMKQGQTLGYVFCGEMAVGAFGLSDSCRSGAKE 541

Query: 541 AIEEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKRE-GPT 600
           A+E++KS GIKTAMLTGDCSAAAM+AQQQL NA+D+IHSELLPK KAN+IK FKR+ G T
Sbjct: 542 AMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKV 660
           AMVGDGLNDT ALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSH KV
Sbjct: 602 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCK 720
           VQNVILSI TK AI+GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRG+DHKHG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKACSTKHGRCNGGDGRLSHRHN--HHHDNHRCNVVDHRSPSLENHDHKHSCSEKKDHG 780
           SSK CSTKHGRC+G + R SH H+  HHH +HRC+VVD +SPS +NH+    C +     
Sbjct: 722 SSKLCSTKHGRCDGSNTRPSHHHHHGHHHHSHRCHVVDDKSPSRDNHN--RDCGD----- 781

Query: 781 LHHLNEHKFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHS-HHVAIDIHESANCERV 839
                  KF HN SNQCEK P E+ENN N   KVD+ NCNCHS HHVAIDIHE  +CERV
Sbjct: 782 -------KFHHNDSNQCEKNPHEKENNGNCSTKVDEPNCNCHSHHHVAIDIHEGTDCERV 829

BLAST of Sed0019519 vs. ExPASy TrEMBL
Match: A0A6J1K632 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492014 PE=3 SV=1)

HSP 1 Score: 1289.2 bits (3335), Expect = 0.0e+00
Identity = 679/847 (80.17%), Postives = 750/847 (88.55%), Query Frame = 0

Query: 1   MAEEAMEK-KKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTII 60
           + E AMEK KKV  ENK +RSHFDVLG+CCSSE+PLIENILKPLEGIK I+VIVPTRT+I
Sbjct: 2   VGEVAMEKNKKVRGENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLY 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+NGKG I KKKWPSPFAIASGLLLTASFLKY+Y
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQVNGKG-ISKKKWPSPFAIASGLLLTASFLKYVY 121

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLF 180
           HPLRWLA+AAVAAGIFPILLKAISA+RHLRID NILA++AVVGTI++NDYMEAGSIVFLF
Sbjct: 122 HPLRWLAIAAVAAGIFPILLKAISAVRHLRIDANILAIVAVVGTISMNDYMEAGSIVFLF 181

Query: 181 SIAEWLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPID 240
           SIAEWLE+ ASHKANAAMSSLMSLAPQKA IAE+GEVV  K+V+L ++LAVK GE IPID
Sbjct: 182 SIAEWLESRASHKANAAMSSLMSLAPQKAIIAETGEVVAVKEVELKSVLAVKGGEVIPID 241

Query: 241 GIVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAEL 300
           GIVVDGKC+VDEKTLTGE+FPVPK KDS+VWAGTINLNGYI+VQTTVVAEDC VAKMAE 
Sbjct: 242 GIVVDGKCDVDEKTLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 301

Query: 301 VEEAQNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKS+TQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSAC
Sbjct: 302 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 361

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFR 420
           PCALILSTPVAAFCALTKAAMAGVLIKG DHLEVLAK+KV+AFDKTGTITRGEF+V++F+
Sbjct: 362 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTNFQ 421

Query: 421 TLQDDISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGK 480
            L+DDIS HTLLHWVSSIESKSSHPMAAAL+NHAKLLSIDIKPE VEEFENFPGEGV GK
Sbjct: 422 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 481

Query: 481 INGNNIYIGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKE 540
           I+G +IYIGS KIAARAGC++   LD+E KQGQT+GY+F G    G+FGLSD+CRSG KE
Sbjct: 482 IDGKDIYIGSTKIAARAGCSSAMKLDEEMKQGQTLGYVFCGEMAVGAFGLSDSCRSGAKE 541

Query: 541 AIEEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKRE-GPT 600
           A+E++KS GIKTAMLTGDCSAAAM+AQQQL NA+D+IHSELLPK KAN+IK FKR+ G T
Sbjct: 542 AMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKV 660
           AMVGDGLNDT ALA ADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSH KV
Sbjct: 602 AMVGDGLNDTPALAIADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCK 720
           VQNVILSI TK AI+GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRG+DHKHG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKACSTKHGRCNGGDGRLS---HRHNHHHDNHRCNVVDHRSPSLENHDHKHSCSEKKDH 780
           SSK CS KHGRC+G + R S   H H+HHH +HRC+VVD +SPS ENH HKH C EKKDH
Sbjct: 722 SSKLCSMKHGRCDGSNTRPSHHHHHHDHHHHSHRCHVVDDKSPSRENHVHKH-CCEKKDH 781

Query: 781 GLHHLNEH------KFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHSHHVAIDIHES 837
            L    +H      KF HNHS           NN N  +KV + NCNCHSHHVAIDIHE 
Sbjct: 782 NLQLPQDHNGDCGDKFHHNHS-----------NNGNCSKKVGEPNCNCHSHHVAIDIHEG 835

BLAST of Sed0019519 vs. ExPASy TrEMBL
Match: A0A6J1EPB0 (cadmium/zinc-transporting ATPase HMA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436148 PE=3 SV=1)

HSP 1 Score: 1288.5 bits (3333), Expect = 0.0e+00
Identity = 674/840 (80.24%), Postives = 748/840 (89.05%), Query Frame = 0

Query: 1   MAEEAMEK-KKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTII 60
           + E AMEK KKV RENK +RSHFDVLG+CCSSE+PLIENILKPLEGIK I+VIVPTRT+I
Sbjct: 2   VGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLY 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+ GKG I KKKWPSPFAIASGLLLTASFLKY+Y
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQLKGKG-ISKKKWPSPFAIASGLLLTASFLKYVY 121

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLF 180
           HPLRWLAVAAVAAGIFPILLKAISA+RHLRIDVNILA+IAVVG +++NDYMEAGSIVFLF
Sbjct: 122 HPLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLF 181

Query: 181 SIAEWLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPID 240
           SIAEWLE+ ASHKANAAM SLMSLAPQKATIAE+GEVV+ K+V L ++LAVK GE IPID
Sbjct: 182 SIAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPID 241

Query: 241 GIVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAEL 300
           GIVVDGKC+VDEK+LTGE+FPVPK KDS+VWAGTINLNGYI+VQTTVVAEDC VAKMAE 
Sbjct: 242 GIVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 301

Query: 301 VEEAQNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKS+TQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSAC
Sbjct: 302 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 361

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFR 420
           PCALILSTPVAAFCALTKAAMAGVLIKG DHLEVLAK+KV+AFDKTGTITRGEF+V+DF+
Sbjct: 362 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQ 421

Query: 421 TLQDDISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGK 480
            L+DDIS HTLLHWVSSIESKSSHPMAAAL+NHAKLLS DIKPE VEEFENFPGEGV GK
Sbjct: 422 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGK 481

Query: 481 INGNNIYIGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKE 540
           I+G +IYIGS KIAARAGC++   L++E KQGQT+GY+F      GSFGLSD+CRSG KE
Sbjct: 482 IDGKDIYIGSTKIAARAGCSSAMKLEEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 541

Query: 541 AIEEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKRE-GPT 600
            +EE+KS GIKTAMLTGDCSAAAM+AQQQL NA+D+IHSELLPK KAN+IK FKR+ G T
Sbjct: 542 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKV 660
           AMVGDGLNDT ALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSH KV
Sbjct: 602 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCK 720
           VQNVILSI TK AI+GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRG+DHKHG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKACSTKHGRCNGGDGRLS--HRHNHHHDNHRCNVVDHRSPSLENHDHKHSCSEKKDHG 780
           SSK CSTKHGRC+G + R S  H H+HHH +HRC+VVD + PS +NH+    C +     
Sbjct: 722 SSKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDNHN--GDCGD----- 781

Query: 781 LHHLNEHKFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHSHHVAIDIHESANCERVE 837
                  KF HN SNQCEK PLE++NN N  +KV + NCNCHSHHVAIDIH+  +CER+E
Sbjct: 782 -------KFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 826

BLAST of Sed0019519 vs. ExPASy TrEMBL
Match: A0A6J1ENN0 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436150 PE=3 SV=1)

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 668/839 (79.62%), Postives = 744/839 (88.68%), Query Frame = 0

Query: 1   MAEEAMEKKKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIV 60
           + E AMEK K   +NK +RSHFDVLG+CCSSE+PLIENILKPLEGIK I+VIVPTRT+IV
Sbjct: 2   VGEVAMEKNK---KNKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIV 61

Query: 61  VHDSLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLYH 120
           VHDSLL+SQLQIVKALNEARLEA VQ+ GKG I KKKWPSPFAIASGLLLTASFLKY+YH
Sbjct: 62  VHDSLLVSQLQIVKALNEARLEAKVQLKGKG-ISKKKWPSPFAIASGLLLTASFLKYVYH 121

Query: 121 PLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFS 180
           PLRWLAVAAVAAGIFPILLKAISA+RHLRIDVNILA+IAVVGT+++NDYMEAGSIVFLFS
Sbjct: 122 PLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEAGSIVFLFS 181

Query: 181 IAEWLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDG 240
           IAEWLE+ ASHKANAAM SLMSLAPQKATIAE+GEVV+ K+V L ++LAVK GE IPIDG
Sbjct: 182 IAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDG 241

Query: 241 IVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELV 300
           IVVDGKC+VDEK+LTGE+FPVPK KDS+VWAGTINLNGYI+VQTT VAEDC VAKMAE V
Sbjct: 242 IVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTAVAEDCVVAKMAEFV 301

Query: 301 EEAQNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACP 360
           EEAQNNKS+TQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSACP
Sbjct: 302 EEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACP 361

Query: 361 CALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRT 420
           CALILSTPVAAFCALTKAAMAGVLIKG DHLEVLAK+KV+AFDKTGTITRGEF+V+DF+ 
Sbjct: 362 CALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQA 421

Query: 421 LQDDISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKI 480
           L+DDIS HTLLHWVSSIESKSSHPMAAAL+NHAKLLS DIKPE VEEFENFPGEGV GKI
Sbjct: 422 LRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGKI 481

Query: 481 NGNNIYIGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEA 540
           +G +IYIGS KIAARAGC++   L++E K GQT+GY+F      GSFGLSD+CRSG KE 
Sbjct: 482 DGKDIYIGSTKIAARAGCSSAMKLEEEMKPGQTLGYVFCEEMAVGSFGLSDSCRSGAKEG 541

Query: 541 IEEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKRE-GPTA 600
           +EE+KS GIKTAMLTGDCSAAAM+AQQQL NA+D+IHSELLPK KAN+IK FKR+ G TA
Sbjct: 542 MEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGATA 601

Query: 601 MVGDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVV 660
           MVGDGLNDT ALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSH KVV
Sbjct: 602 MVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVV 661

Query: 661 QNVILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCKS 720
           QNVILSI TK AI+GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRG+DHKHG KCCKS
Sbjct: 662 QNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCKS 721

Query: 721 SKACSTKHGRCNGGDGRLS--HRHNHHHDNHRCNVVDHRSPSLENHDHKHSCSEKKDHGL 780
           SK CSTKHGRC+G + R S  H H+HHH +HRC+V+D +SPS +NH+    C +      
Sbjct: 722 SKLCSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVIDDKSPSRDNHN--GDCGD------ 781

Query: 781 HHLNEHKFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHSHHVAIDIHESANCERVE 837
                 KF HN SNQCEK PLE++NN N  +KV + NCNCHSHHVAIDIH+  +CER+E
Sbjct: 782 ------KFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 822

BLAST of Sed0019519 vs. ExPASy TrEMBL
Match: A0A6J1K3E5 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492014 PE=3 SV=1)

HSP 1 Score: 1270.8 bits (3287), Expect = 0.0e+00
Identity = 669/841 (79.55%), Postives = 741/841 (88.11%), Query Frame = 0

Query: 1   MAEEAMEK-KKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTII 60
           + E AMEK KKV  ENK +RSHFDVLG+CCSSE+PLIENILKPLEGIK I+VIVPTRT+I
Sbjct: 2   VGEVAMEKNKKVRGENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLY 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+NGKG I KKKWPSPFAIASGLLLTASFLKY+Y
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQVNGKG-ISKKKWPSPFAIASGLLLTASFLKYVY 121

Query: 121 HPLRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLF 180
           HPLRWLA+AAVAAGIFPILLKAISA+RHLRID NILA++AVVGTI++NDYMEAGSIVFLF
Sbjct: 122 HPLRWLAIAAVAAGIFPILLKAISAVRHLRIDANILAIVAVVGTISMNDYMEAGSIVFLF 181

Query: 181 SIAEWLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPID 240
           SIAEWLE+ ASHKANAAMSSLMSLAPQKA IAE+GEVV  K+V+L ++LAVK GE IPID
Sbjct: 182 SIAEWLESRASHKANAAMSSLMSLAPQKAIIAETGEVVAVKEVELKSVLAVKGGEVIPID 241

Query: 241 GIVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAEL 300
           GIVVDGKC+VDEKTLTGE+FPVPK KDS+VWAGTINLNGYI+VQTTVVAEDC VAKMAE 
Sbjct: 242 GIVVDGKCDVDEKTLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEF 301

Query: 301 VEEAQNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSAC 360
           VEEAQNNKS+TQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSAC
Sbjct: 302 VEEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSAC 361

Query: 361 PCALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFR 420
           PCALILSTPVAAFCALTKAAMAGVLIKG DHLEVLAK+KV+AFDKTGTITRGEF+V++F+
Sbjct: 362 PCALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTNFQ 421

Query: 421 TLQDDISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGK 480
            L+DDIS HTLLHWVSSIESKSSHPMAAAL+NHAKLLSIDIKPE VEEFENFPGEGV GK
Sbjct: 422 ALRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGK 481

Query: 481 INGNNIYIGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKE 540
           I+G +IYIGS KIAARAGC++   LD+E KQGQT+GY+F G    G+FGLSD+CRSG KE
Sbjct: 482 IDGKDIYIGSTKIAARAGCSSAMKLDEEMKQGQTLGYVFCGEMAVGAFGLSDSCRSGAKE 541

Query: 541 AIEEIKSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKRE-GPT 600
           A+E++KS GIKTAMLTGDCSAAAM+AQQQL NA+D+IHSELLPK KAN+IK FKR+ G T
Sbjct: 542 AMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKV 660
           AMVGDGLNDT ALA ADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSH KV
Sbjct: 602 AMVGDGLNDTPALAIADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIVTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCK 720
           VQNVILSI TK AI+GLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRG+DHKHG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKACSTKHGRCNGGDGRLS---HRHNHHHDNHRCNVVDHRSPSLENHDHKHSCSEKKDH 780
           SSK CS KHGRC+G + R S   H H+HHH +HRC+VVD +SPS ENH+    C +    
Sbjct: 722 SSKLCSMKHGRCDGSNTRPSHHHHHHDHHHHSHRCHVVDDKSPSRENHN--GDCGD---- 781

Query: 781 GLHHLNEHKFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHSHHVAIDIHESANCERV 837
                   KF HNHS           NN N  +KV + NCNCHSHHVAIDIHE  +CER+
Sbjct: 782 --------KFHHNHS-----------NNGNCSKKVGEPNCNCHSHHVAIDIHEGIDCERI 816

BLAST of Sed0019519 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 961.1 bits (2483), Expect = 6.0e-280
Identity = 507/803 (63.14%), Postives = 631/803 (78.58%), Query Frame = 0

Query: 12  MRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIVVHDSLLISQLQ 71
           M   K+ +S+FDVLG+CC+SE+PLIENIL  ++G+K+ +VIVP+RT+IVVHD+L++SQ Q
Sbjct: 1   MASKKMTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQ 60

Query: 72  IVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLYHPLRWLAVAAVA 131
           IVKALN+A+LEANV++ G+    K KWPSPFA+ SG+LL  SF KYLY P RWLAVAAV 
Sbjct: 61  IVKALNQAQLEANVRVTGETNF-KNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVV 120

Query: 132 AGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFSIAEWLETLASH 191
           AGI+PIL KA++++   RID+NIL V+ V  TI + DY EA  +VFLF+IAEWL++ AS+
Sbjct: 121 AGIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASY 180

Query: 192 KANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDGIVVDGKCEVDE 251
           KA+A M SLMSLAPQKA IAE+GE V+  ++K NT++AVKAGE IPIDG+VVDG CEVDE
Sbjct: 181 KASAVMQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDE 240

Query: 252 KTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELVEEAQNNKSRTQ 311
           KTLTGE+FPVPK KDS VWAGTINLNGYITV TT +AEDC VAKMA+LVEEAQN+K+ TQ
Sbjct: 241 KTLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQ 300

Query: 312 RFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACPCALILSTPVAA 371
           RFID+C+KYYTPA+++IS C  AIP A++VHNL HW HLALVVLVSACPC LILSTPVA 
Sbjct: 301 RFIDKCSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVAT 360

Query: 372 FCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRTLQDDISLHTLL 431
           FCALTKAA +G+LIKG D+LE LAK+K++AFDKTGTITRGEF+V DF++L +DISL +LL
Sbjct: 361 FCALTKAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSLSEDISLQSLL 420

Query: 432 HWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKINGNNIYIGSRK 491
           +WVSS ESKSSHPMAAA++++A+ +S++ KPE VE+++NFPGEG++GKI+G  +YIG+++
Sbjct: 421 YWVSSTESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKR 480

Query: 492 IAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEAIEEIKSFGIKT 551
           IA+RAGC +VP +D +TK G+TIGY++ G TLAG F LSDACRSGV +A++E+KS GIK 
Sbjct: 481 IASRAGCLSVPDIDVDTKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLGIKI 540

Query: 552 AMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKR-EGPTAMVGDGLNDTLA 611
           AMLTGD  AAAM+AQ+QLGNA+DI+ +ELLP+ K+ IIK  KR EGPTAMVGDGLND  A
Sbjct: 541 AMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMVGDGLNDAPA 600

Query: 612 LATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVVQNVILSIVTKT 671
           LATADIGISMGVSGSALATETGN+ILMSND+R+IP+AIKLAK++  KVV+NV++SI  K 
Sbjct: 601 LATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKG 660

Query: 672 AIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCKSSKACSTKHGRC 731
           AI+ LA AGHPL+WAAVLADVGTCLLVILNSMLLL    HK G KC + S + S      
Sbjct: 661 AILALAFAGHPLIWAAVLADVGTCLLVILNSMLLL-SDKHKTGNKCYRESSSSSVLIAEK 720

Query: 732 NGGDGRLSHRHNHHHDNHRCNVVDHRSPSLENHDHKH----SCSEKKDHGLHHLNEHKFD 791
             GD                   D  +  L     KH     C  K         +   D
Sbjct: 721 LEGDA----------------AGDMEAGLLPKISDKHCKPGCCGTKTQEKAMKPAKASSD 780

Query: 792 HNHSNQCEKKPLEQENNANYLRK 810
           H+HS  CE K   Q++N   ++K
Sbjct: 781 HSHSGCCETK---QKDNVTVVKK 782

BLAST of Sed0019519 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 959.5 bits (2479), Expect = 1.7e-279
Identity = 509/832 (61.18%), Postives = 655/832 (78.73%), Query Frame = 0

Query: 7   EKKKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIVVHDSLL 66
           EKKKV    KLQ+S+FDVLG+CC+SE+P+IENILK L+G+K+ +VIVP+RT+IVVHDSLL
Sbjct: 9   EKKKV---KKLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSLL 68

Query: 67  ISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLYHPLRWLA 126
           IS  QI KALNEARLEANV++NG+    K KWPSPFA+ SGLLL  SFLK++Y PLRWLA
Sbjct: 69  ISPFQIAKALNEARLEANVRVNGETSF-KNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLA 128

Query: 127 VAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFSIAEWLE 186
           VAAVAAGI+PIL KA ++I+  RID+NIL +I V+ T+A+ D+MEA ++VFLF+I++WLE
Sbjct: 129 VAAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLE 188

Query: 187 TLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDGIVVDGK 246
           T AS+KA + M SLMSLAPQKA IAE+GE V+  +VK++T++AVKAGE IPIDGIVVDG 
Sbjct: 189 TRASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGN 248

Query: 247 CEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELVEEAQNN 306
           CEVDEKTLTGE+FPVPKQ+DS VWAGTINLNGYI V+TT +A DC VAKMA+LVEEAQ++
Sbjct: 249 CEVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSS 308

Query: 307 KSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACPCALILS 366
           K+++QR ID+C++YYTPA++++SAC+A +P  M+VHNL HWFHLALVVLVS CPC LILS
Sbjct: 309 KTKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILS 368

Query: 367 TPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRTLQDDIS 426
           TPVA FCALTKAA +G+LIK  D+L+ L+K+K++AFDKTGTITRGEF+V DF++L  DI+
Sbjct: 369 TPVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRDIN 428

Query: 427 LHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKINGNNIY 486
           L +LL+WVSS+ESKSSHPMAA ++++AK +S++ +PE VE+++NFPGEG++GKI+GN+I+
Sbjct: 429 LRSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIF 488

Query: 487 IGSRKIAARAGCTTVPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEAIEEIKS 546
           IG++KIA+RAGC+TVP ++ +TK G+T+GY++ G  LAG F LSDACRSGV +A+ E+KS
Sbjct: 489 IGNKKIASRAGCSTVPEIEVDTKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKS 548

Query: 547 FGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKREGPTAMVGDGLN 606
            GIKTAMLTGD  AAAM+AQ+QLGN LD++H +LLP+ K+ II+ FK+EGPTAMVGDG+N
Sbjct: 549 LGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKEGPTAMVGDGVN 608

Query: 607 DTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVVQNVILSI 666
           D  ALATADIGISMG+SGSALAT+TGN+ILMSND+R+IP+A+KLA+++  KVV+NV LSI
Sbjct: 609 DAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSI 668

Query: 667 VTKTAIIGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHKHGKKCCKSSKACSTK 726
           + K  I+ LA AGHPL+WAAVL DVGTCLLVI NSMLLLR       KKC ++S   S  
Sbjct: 669 ILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKKCYRAS--TSKL 728

Query: 727 HGRCNGGD---------GRLSHRHNHHHDNHRC-------NVVDHRSPSLENHDHKH--S 786
           +GR   GD         G L+   N    +  C       NVV  +  S  + DH H   
Sbjct: 729 NGRKLEGDDDYVVDLEAGLLTKSGNGQCKSSCCGDKKNQENVVMMKPSSKTSSDHSHPGC 788

Query: 787 CSEKKDHGLHHLNEHKFDHNHSNQCEKKPLEQENNANYLRKVDKSNCNCHSH 821
           C +KK+  +  L +          C +K  + E +      V  S+C   SH
Sbjct: 789 CGDKKEEKVKPLVK-------DGCCSEKTRKSEGD-----MVSLSSCKKSSH 822

BLAST of Sed0019519 vs. TAIR 10
Match: AT4G30120.1 (heavy metal atpase 3 )

HSP 1 Score: 677.9 bits (1748), Expect = 1.0e-194
Identity = 339/545 (62.20%), Postives = 443/545 (81.28%), Query Frame = 0

Query: 4   EAMEKKKVMRENKLQRSHFDVLGLCCSSEIPLIENILKPLEGIKDITVIVPTRTIIVVHD 63
           E  E KK+     LQ S+FDV+G+CCSSE+ ++ N+L+ ++G+K+ +VIVP+RT+IVVHD
Sbjct: 3   EGEESKKM----NLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSRTVIVVHD 62

Query: 64  SLLISQLQIVKALNEARLEANVQINGKGLIRKKKWPSPFAIASGLLLTASFLKYLYHPLR 123
           + LIS LQIVKALN+ARLEA+V+  G+  + K +WPSPFAI SG+LL  SF KY Y PL 
Sbjct: 63  TFLISPLQIVKALNQARLEASVRPYGETSL-KSQWPSPFAIVSGVLLVLSFFKYFYSPLE 122

Query: 124 WLAVAAVAAGIFPILLKAISAIRHLRIDVNILAVIAVVGTIALNDYMEAGSIVFLFSIAE 183
           WLA+ AV AG+FPIL KA++++   R+D+N L +IAV+ T+ + D+ EA +IVFLFS+A+
Sbjct: 123 WLAIVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIVFLFSVAD 182

Query: 184 WLETLASHKANAAMSSLMSLAPQKATIAESGEVVDSKDVKLNTILAVKAGEAIPIDGIVV 243
           WLE+ A+HKA+  MSSLMSLAP+KA IA++G  VD  +V +NT+++VKAGE+IPIDG+VV
Sbjct: 183 WLESSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESIPIDGVVV 242

Query: 244 DGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGYITVQTTVVAEDCAVAKMAELVEEA 303
           DG C+VDEKTLTGESFPV KQ++S V A TINLNGYI V+TT +A DC VAKM +LVEEA
Sbjct: 243 DGSCDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKMTKLVEEA 302

Query: 304 QNNKSRTQRFIDECAKYYTPAVLVISACLAAIPAAMRVHNLSHWFHLALVVLVSACPCAL 363
           Q ++++TQRFID+C++YYTPAV+V +AC A IP  ++V +LSHWFHLALVVLVS CPC L
Sbjct: 303 QKSQTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLVSGCPCGL 362

Query: 364 ILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRTLQD 423
           ILSTPVA FCALTKAA +G LIK GD LE LAK+K++AFDKTGTIT+ EF+VSDFR+L  
Sbjct: 363 ILSTPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVSDFRSLSP 422

Query: 424 DISLHTLLHWVSSIESKSSHPMAAALINHAKLLSIDIKPENVEEFENFPGEGVHGKINGN 483
            I+LH LL+WVSSIE KSSHPMAAALI++A+ +S++ KP+ VE F+NFPGEGV+G+I+G 
Sbjct: 423 SINLHKLLYWVSSIECKSSHPMAAALIDYARSVSVEPKPDIVENFQNFPGEGVYGRIDGQ 482

Query: 484 NIYIGSRKIAARAGCTT--VPSLDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEAI 543
           +IYIG+++IA RAGC T  VP ++   K+G+TIGYI+ GA L GSF L D CR GV +A+
Sbjct: 483 DIYIGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGCRYGVAQAL 542

Query: 544 EEIKS 547
           +E+KS
Sbjct: 543 KELKS 542

BLAST of Sed0019519 vs. TAIR 10
Match: AT5G44790.1 (copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPase (RAN1) )

HSP 1 Score: 248.4 bits (633), Expect = 2.0e-65
Identity = 187/591 (31.64%), Postives = 311/591 (52.62%), Query Frame = 0

Query: 169 YMEAGSIVFLF-SIAEWLETLASHKANAAMSSLMSLAPQKA---TIAESGEVVDSKDVKL 228
           Y +A +++  F  + ++LE+LA  K + AM  L+ L P  A   T  + G++V  +++  
Sbjct: 402 YFDASAMLITFVLLGKYLESLAKGKTSDAMKKLVQLTPATAILLTEGKGGKLVGEREIDA 461

Query: 229 NTI-----LAVKAGEAIPIDGIVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNGY 288
             I     L V  G  IP DG+VV G   V+E  +TGES PV K+ DS V  GTIN++G 
Sbjct: 462 LLIQPGDTLKVHPGAKIPADGVVVWGSSYVNESMVTGESVPVSKEVDSPVIGGTINMHGA 521

Query: 289 ITVQTTVVAEDCAVAKMAELVEEAQNNKSRTQRFIDECAKYYTPAVLV----------IS 348
           + ++ T V  D  ++++  LVE AQ +K+  Q+F D  A  + P V+           I 
Sbjct: 522 LHMKATKVGSDAVLSQIISLVETAQMSKAPIQKFADYVASIFVPVVITLALFTLVGWSIG 581

Query: 349 ACLAAIPAAMRVHNLSHW---FHLALVVLVSACPCALILSTPVAAFCALTKAAMAGVLIK 408
             + A P      N +H+      ++ V+V ACPCAL L+TP A   A    A  GVLIK
Sbjct: 582 GAVGAYPDEWLPENGTHFVFSLMFSISVVVIACPCALGLATPTAVMVATGVGATNGVLIK 641

Query: 409 GGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRTLQDDISLHTLLHWVSSIESKSSHPMA 468
           GGD LE   KVK + FDKTGT+T+G+  V+  +    ++     L  V+S E+ S HP+A
Sbjct: 642 GGDALEKAHKVKYVIFDKTGTLTQGKATVTTTKVF-SEMDRGEFLTLVASAEASSEHPLA 701

Query: 469 AALINHAKLLS-IDIKPENVE----------------EFENFPGEGVHGKINGNNIYIGS 528
            A++ +A+     D   E+ E                +F   PG+G+   +N   I +G+
Sbjct: 702 KAIVAYARHFHFFDESTEDGETNNKDLQNSGWLLDTSDFSALPGKGIQCLVNEKMILVGN 761

Query: 529 RKIAARAGCTTVPS-----LDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEAIEEI 588
           RK+ +      +P      ++D  + G+T   + Y   L G  G++D  +      +E +
Sbjct: 762 RKLMSE-NAINIPDHVEKFVEDLEESGKTGVIVAYNGKLVGVMGIADPLKREAALVVEGL 821

Query: 589 KSFGIKTAMLTGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKREGPT-AMVGD 648
              G++  M+TGD    A    +++G  ++ + +E++P GKA++I+  +++G T AMVGD
Sbjct: 822 LRMGVRPIMVTGDNWRTARAVAKEVG--IEDVRAEVMPAGKADVIRSLQKDGSTVAMVGD 881

Query: 649 GLNDTLALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVVQNVI 707
           G+ND+ ALA AD+G+++G +G+ +A E  + +LM N++  +  AI L++K+  ++  N +
Sbjct: 882 GINDSPALAAADVGMAIG-AGTDVAIEAADYVLMRNNLEDVITAIDLSRKTLTRIRLNYV 941

BLAST of Sed0019519 vs. TAIR 10
Match: AT1G63440.1 (heavy metal atpase 5 )

HSP 1 Score: 230.3 bits (586), Expect = 5.6e-60
Identity = 181/591 (30.63%), Postives = 303/591 (51.27%), Query Frame = 0

Query: 168 DYMEAGSIVFLFSI-AEWLETLASHKANAAMSSLMSLAPQKATI--------AESGEVVD 227
           D+ E  +++  F I  ++LE +A  K + A++ LM+LAP  A +            E +D
Sbjct: 404 DFFETSAMLISFIILGKYLEVMAKGKTSQAIAKLMNLAPDTAILLSLDKEGNVTGEEEID 463

Query: 228 SKDVKLNTILAVKAGEAIPIDGIVVDGKCEVDEKTLTGESFPVPKQKDSIVWAGTINLNG 287
            + ++ N ++ +  G  +  DG V+ G+  V+E  +TGE+ PV K+K   V  GT+N NG
Sbjct: 464 GRLIQKNDVIKIVPGAKVASDGYVIWGQSHVNESMITGEARPVAKRKGDTVIGGTLNENG 523

Query: 288 YITVQTTVVAEDCAVAKMAELVEEAQNNKSRTQRFIDECAKYYTPAVLVISACL------ 347
            + V+ T V  + A+A++  LVE AQ  K+  Q+  D  +K++ P V+ +S         
Sbjct: 524 VLHVKVTRVGSESALAQIVRLVESAQLAKAPVQKLADRISKFFVPLVIFLSFSTWLAWFL 583

Query: 348 ---------AAIPAAMRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALTKAAMAGV 407
                    + IP++M    L+  F ++  V+V ACPCAL L+TP A        A  GV
Sbjct: 584 AGKLHWYPESWIPSSMDSFELALQFGIS--VMVIACPCALGLATPTAVMVGTGVGASQGV 643

Query: 408 LIKGGDHLEVLAKVKVMAFDKTGTITRGEFLVSDFRTLQDDISLHTLLHWVSSIESKSSH 467
           LIKGG  LE   KV  + FDKTGT+T G+ +V   + L++ + L      V++ E  S H
Sbjct: 644 LIKGGQALERAHKVNCIVFDKTGTLTMGKPVVVKTKLLKNMV-LREFYELVAATEVNSEH 703

Query: 468 PMAAALINHAKLLSIDIK----PENVEEFENFPGEGVHGKINGNNIYIGSRKIAARAGCT 527
           P+A A++ +AK    D +    PE   +F +  G+GV   + G  I +G++ +       
Sbjct: 704 PLAKAIVEYAKKFRDDEENPAWPEAC-DFVSITGKGVKATVKGREIMVGNKNL-MNDHKV 763

Query: 528 TVPS-----LDDETKQGQTIGYIFYGATLAGSFGLSDACRSGVKEAIEEIKSFGIKTAML 587
            +P      L D     QT   +   + L G   +SD  +   +EAI  +KS  IK+ M+
Sbjct: 764 IIPDDAEELLADSEDMAQTGILVSINSELIGVLSVSDPLKPSAREAISILKSMNIKSIMV 823

Query: 588 TGDCSAAAMYAQQQLGNALDIIHSELLPKGKANIIKGFKREG-PTAMVGDGLNDTLALAT 647
           TGD    A    +++G  +D + +E  P+ KA  +K  +  G   AMVGDG+ND+ AL  
Sbjct: 824 TGDNWGTANSIAREVG--IDSVIAEAKPEQKAEKVKELQAAGHVVAMVGDGINDSPALVA 883

Query: 648 ADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHVKVVQNVILSIVTKTAII 707
           AD+G+++G +G+ +A E  +++LM +++  +  AI L++K+  ++  N + ++     ++
Sbjct: 884 ADVGMAIG-AGTDIAIEAADIVLMKSNLEDVITAIDLSRKTFSRIRLNYVWALGYN--LM 943

Query: 708 GLAIAGHPLV---------WAAVLADVGTCLLVILNSMLLLRGTDHKHGKK 716
           G+ IA   L          W A  A   + + V+  S+LL    ++K  KK
Sbjct: 944 GIPIAAGVLFPGTRFRLPPWIAGAAMAASSVSVVCCSLLL---KNYKRPKK 981