Lag0007527 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0007527
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Reverse transcriptase domain-containing protein
Location	chr9: 705240 .. 707679 (+)
RNA-Seq Expression	Lag0007527
Synteny	Lag0007527

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCGAGGAGTTCCTCCTCCATCCGCCGTTTTGGGAGAAGGGAAGATTTCTTTGGCATGCTGGCATTTGTGCTATTTTGTGGGGTTTATAGGGGGAAAGAAATAATATAATTTTTAGAGGGTTTGAGAGACATCCTTGTGAGGTTTGGACTAATATTAAATTTTATGTTTTTTTATGGGTCTCGGTTGACAAGACTTTCTGTAATTACCCTCTTGGTCTTATTTTGATTGATCGGAATCCCTTCCTTTAGTTGGGTTCCTTCAATGGGGCTTGGTTTTTTTTGGGTGCCCTTGTATTCTTTCATTTTTTCTCAATGAAAGCTCGGTTATTTATAAAAAAAAAAAAAGGGTCCCACTAAGGTGATAAACAAGATTGAAAAGCTCTTTCGAAATTCTTATGGAGGGGAAAAAATGACTCAAACGGATTCCACCTTTTGAGATGGGATCGGGTAAAAGCTCCTATCGAGGAAGGTGGCTTTGGTATCATGGGAATTCAACAGAAAAACTCATCTCTTCTGGCTAAATGGATTTGGTGTTATCATATTGAAAAAGAGCTCTATGGCGTCGGGTCATTGCTTCTAAATTAAGGACCACACACTTCGATCTAAAGCTGGGTGCCAAAACCTTAAACTCATCCGAAGGACTATGGAAATCTATTGATCATGTGAAACATATGGTGTATTCCAACATATCGATAAAGGTGGGTGATGGTAAAAGTACCTCTTTCTGGAAAGACTCATGGATGGGGAACTCTAACCTCCAATTCATTTATCTTCGACTTTATCATTTATCGACCAAGAAAGATGCACCTATTGCTGATTTTTGGTGTCCACAAACCCATACTTGGATGCTTCACACTAGAAGAAACCTTTACTGATTTGGAGGCAGAGGATTGAATATCGCTTTTGTCCCATTTGCAGACGATGGTTAACAACAACAAAGAGGATTCGTGAATGCAGTTCTTCAAGCCAAAGGTTTTGGTTTGATTTGGAGAAAATGGATTTGTGGATGCATTTCAAGTGTTAACTACTCTATTATCATTAATGGTAGACCTAGGGAAAAAATTATTCCTTCTCGTGGTATTCGCCAAGGTGATCCTCTTTCTCTTTTTCTGTTCATATTGGTTTCTGATTGCCTCAATCGTCTTTTATCTCACAGTGCAAGATTGGGTAGAATTATTTCTCATCCAATAGGTAACTCTCGTCTCTCATTGACACACTTGCAATTTGCGGATGATACTCTTCTTTTCTCCGTCTATGATTCTAAAGCATTGGATAATCTTTTTGAGATTATCAAACTCTTTGAGATGGCTTTTGGTTTGAACATCAATTTTGCTAAGAGTGAGCTTTTGGGGATTCACATTGTCGACTCAGATATGGAATGTTTGACAGCAAAATTTGGTTGTAAGTAGGGTTCATGGCCTTCCACATATCTGGGGCTTCCTTTGGGGGCAATTCGAAAGGTTCTCAGTTTTGGCAGCCTGTTATTGAAAGAATCCAACATAAGCTTCATAACTGGAAATACTCTTTTATCTCCAAAGGTGGTAGGCACACCCTCATCCAATCTATCTCTCAAGTATGCCCACATATTACTTATCCTTATTTAAATTACCTTCTAAGGTTGCAAAATCTCTGGATAAGCTTATTCGAGATTTTTTTTTGGGAGGGCTCCAGAGGCGAGGGCGGCATGCATAATGTAAATTGGGAGACAACGCAGCTTCCAAAATTTATGGGTGGAGTAAGCATTGGAAATTTCCATCACCGTAATTTGGCTCTCTTATCAAAATGGATTTGGAGGTTTTTACATGAAGGTAATGCCCTTTGGCGCCAACTTATTATTGCTAAATATTATTACTCAGAATCAACTTGTATTTGGCCTAAACATATGTAGAGAGGATCTTTTAAATCTCCTTGGCGTTTTATTTGTTCTACCATAGATCTTGTTGCTAGTCGTATTCAGAGACGGCTTGGAAATGGTTGTTCCACCCTTTTTTGGCATGATTCTTGGCTAAGTTGTGGAGTCTTGTCTGAGGCTTTCCCTCGTCTTTATCGATTATCTAGTCGTTCGGAAGGTACAGTTGCTGACTTTTGGGTTTCATTGAATTCAGCTTGGGATTTGAGTCTTCGTCGAAATTTAAATGATTCGGAGACAAATGAGTGGGCTAGTCTCTCTTATCTGCTTTCTTTCATCAGAATTCGAGTTATTGATGACACTTGGTCTTGGCCGATTGATTCGTCTAATGCATTCACAGTTAAATCTCTTATGGGAGATATGGTTGGTGATTCTGACCCCACATCGAGCAAATTATATAATGTGGTGTGGAAAGACGTTTATCCAAAGAAGATCAAAATTTTTATCTGGGAGCTTAGTCTTGGAGCTATTAATACGTCTGATCGACTTCAAAGACGAATGCCTTATTTGCACCTTTCTCCATCCTAG

mRNA sequence

ATGACCGAGGAGTTCCTCCTCCATCCGCCGTTTTGGGAGAAGGGAAGATTTCTTTGGCATGCTGGCATTTGTGCTATTTTGTGGGTTCTTCAAGCCAAAGGTTTTGGTTTGATTTGGAGAAAATGGATTTGTGGATGCATTTCAAGTGTTAACTACTCTATTATCATTAATGGTAGACCTAGGGAAAAAATTATTCCTTCTCGTGGTATTCGCCAAGGTGATCCTCTTTCTCTTTTTCTGTTCATATTGGTTTCTGATTGCCTCAATCGTCTTTTATCTCACAGTGCAAGATTGGGTAGAATTATTTCTCATCCAATAGGTAACTCTCGTCTCTCATTGACACACTTGCAATTTGCGGATGATACTCTTCTTTTCTCCGTCTATGATTCTAAAGCATTGGATAATCTTTTTGAGATTATCAAACTCTTTGAGATGGCTTTTGGTTTGAACATCAATTTTGCTAAGAGTGAGCTTTTGGGGATTCACATTGTCGACTCAGATATGGAATGTTTGACAGCAAAATTTGGTTATCTTGTTGCTAGTCGTATTCAGAGACGGCTTGGAAATGGTTGTTCCACCCTTTTTTGGCATGATTCTTGGCTAAGTTGTGGAGTCTTGTCTGAGGCTTTCCCTCGTCTTTATCGATTATCTAGTCGTTCGGAAGGTACAGTTGCTGACTTTTGGGTTTCATTGAATTCAGCTTGGGATTTGAGTCTTCGTCGAAATTTAAATGATTCGGAGACAAATGAGTGGGCTAGTCTCTCTTATCTGCTTTCTTTCATCAGAATTCGAGTTATTGATGACACTTGGTCTTGGCCGATTGATTCGTCTAATGCATTCACAGTTAAATCTCTTATGGGAGATATGGTTGGTGATTCTGACCCCACATCGAGCAAATTATATAATGTGGTGTGGAAAGACGTTTATCCAAAGAAGATCAAAATTTTTATCTGGGAGCTTAGTCTTGGAGCTATTAATACGTCTGATCGACTTCAAAGACGAATGCCTTATTTGCACCTTTCTCCATCCTAG

Coding sequence (CDS)

ATGACCGAGGAGTTCCTCCTCCATCCGCCGTTTTGGGAGAAGGGAAGATTTCTTTGGCATGCTGGCATTTGTGCTATTTTGTGGGTTCTTCAAGCCAAAGGTTTTGGTTTGATTTGGAGAAAATGGATTTGTGGATGCATTTCAAGTGTTAACTACTCTATTATCATTAATGGTAGACCTAGGGAAAAAATTATTCCTTCTCGTGGTATTCGCCAAGGTGATCCTCTTTCTCTTTTTCTGTTCATATTGGTTTCTGATTGCCTCAATCGTCTTTTATCTCACAGTGCAAGATTGGGTAGAATTATTTCTCATCCAATAGGTAACTCTCGTCTCTCATTGACACACTTGCAATTTGCGGATGATACTCTTCTTTTCTCCGTCTATGATTCTAAAGCATTGGATAATCTTTTTGAGATTATCAAACTCTTTGAGATGGCTTTTGGTTTGAACATCAATTTTGCTAAGAGTGAGCTTTTGGGGATTCACATTGTCGACTCAGATATGGAATGTTTGACAGCAAAATTTGGTTATCTTGTTGCTAGTCGTATTCAGAGACGGCTTGGAAATGGTTGTTCCACCCTTTTTTGGCATGATTCTTGGCTAAGTTGTGGAGTCTTGTCTGAGGCTTTCCCTCGTCTTTATCGATTATCTAGTCGTTCGGAAGGTACAGTTGCTGACTTTTGGGTTTCATTGAATTCAGCTTGGGATTTGAGTCTTCGTCGAAATTTAAATGATTCGGAGACAAATGAGTGGGCTAGTCTCTCTTATCTGCTTTCTTTCATCAGAATTCGAGTTATTGATGACACTTGGTCTTGGCCGATTGATTCGTCTAATGCATTCACAGTTAAATCTCTTATGGGAGATATGGTTGGTGATTCTGACCCCACATCGAGCAAATTATATAATGTGGTGTGGAAAGACGTTTATCCAAAGAAGATCAAAATTTTTATCTGGGAGCTTAGTCTTGGAGCTATTAATACGTCTGATCGACTTCAAAGACGAATGCCTTATTTGCACCTTTCTCCATCCTAG

Protein sequence

MTEEFLLHPPFWEKGRFLWHAGICAILWVLQAKGFGLIWRKWICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDCLNRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAFGLNINFAKSELLGIHIVDSDMECLTAKFGYLVASRIQRRLGNGCSTLFWHDSWLSCGVLSEAFPRLYRLSSRSEGTVADFWVSLNSAWDLSLRRNLNDSETNEWASLSYLLSFIRIRVIDDTWSWPIDSSNAFTVKSLMGDMVGDSDPTSSKLYNVVWKDVYPKKIKIFIWELSLGAINTSDRLQRRMPYLHLSPS

Homology

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BLAST of Lag0007527 vs. NCBI nr
Match: XP_008446987.1 (PREDICTED: uncharacterized protein LOC103489530 [Cucumis melo])

HSP 1 Score: 194.5 bits (493), Expect = 1.5e-45
Identity = 131/409 (32.03%), Postives = 194/409 (47.43%), Query Frame = 0


Query: 28  WVLQAKGFGLIWRKWICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDC 87
           +VL+ K F  +WRKWI GCIS+V YS+I+NGRP+ +I  +RG+RQGDPLS FLF++  D 
Sbjct: 125 FVLEKKNFPNLWRKWIKGCISNVTYSVIVNGRPQGRIKANRGLRQGDPLSPFLFVIAMDY 184

Query: 88  LNRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAF 147
           L+RLLSH    G I    + NS  +++H+ FA D LLF   +   L+NL   + LFE A 
Sbjct: 185 LSRLLSHLESSGAIKGVSL-NSNCNISHILFAYDILLFIEDNDCFLNNLRMALSLFERAS 244

Query: 148 GLNINFAKSELLGIHI-VDSDMECLT------AKFGYLV--------------------- 207
           GL IN  KS L+ +++ ++   EC +      ++  YL+                     
Sbjct: 245 GLKINLLKSALVPVNVSLNRAKECASFWGNNGSEGSYLINWTKVFKSKEEGGLGISRLHV 304

Query: 208 ----------------ASRIQRR------------------------------------- 267
                            + + RR                                     
Sbjct: 305 TNKALLSKWLWCYLSEPNALWRRLIQCKYKGNYPGDIPSNISSSTSKAPWRSIIDNIDWF 364

Query: 268 -------LGNGCSTLFWHDSWLSCGVLSEAFPRLYRLSSRSEGTVADFWVSLNSAWDLSL 327
                  L NG    FW+ +W   G LS A+PRL+ L+   E +V D W + ++ W++  
Sbjct: 365 KSNQSWELNNGDQISFWYSNWSQEGHLSTAYPRLFALTLGKEISVKDAWNTFDNQWNIIF 424

Query: 328 RRNLNDSETNEWASLSYLLSFIRIRVIDDTWSWPIDSSNAFTV---KSLMGDMVGDS--D 344
           RR LND E  +WA +  +L   R        +W  DS+N+F++   K L+   +  S  D
Sbjct: 425 RRELNDRERCKWAKILEILPTPRSNRRSSKPTWTPDSNNSFSIASAKVLISRQLDQSPRD 484

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BLAST of Lag0007527 vs. NCBI nr
Match: KAA0044451.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 193.7 bits (491), Expect = 2.5e-45
Identity = 128/361 (35.46%), Postives = 176/361 (48.75%), Query Frame = 0


Query: 43  ICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDCLNRLLSHSARLGRII 102
           I  CISSV YSI+INGRPR +I PSRGIRQGDPLS F+F+L  D L+RLL++ A   R I
Sbjct: 2   IASCISSVQYSILINGRPRGRIKPSRGIRQGDPLSPFIFVLAMDYLSRLLNNLAD-KRKI 61

Query: 103 SHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAFGLNINFAKSELLGIH 162
           +    +  L+LTH+ FADD L+F       + NL  I+ LFE A GLNIN +KS +  I+
Sbjct: 62  NGVNFSPNLNLTHILFADDILIFVEDRDDYVSNLKMILHLFESASGLNINLSKSTIFPIN 121

Query: 163 IVDSDMECLTAKFG---------YL----------------VASRIQRRL---------- 222
           +     + +   +G         YL                V  +IQ++L          
Sbjct: 122 VPTDPAKSIADSWGISKGHLPTSYLGMPLGGKPSSSNFWDNVLQKIQKKLSSWKYSQLSK 181

Query: 223 ----------------------GNGCSTLFWHDSWLSCGVLSEAFPRLYRLSSRSEGTVA 282
                                  +G    FW D+W     LS   PRL+ LS+  +G+V 
Sbjct: 182 GGRITLINSTLESLPIYQIWKVNDGEDISFWLDNWNGNAPLSLVVPRLFALSTNKKGSVK 241

Query: 283 DFWVSLNSAWDLSLRRNLNDSETNEWASLSYLLSFIRIRVIDDTWSWPIDSSNAF---TV 342
           DFW   ++ W L + R L D E N W ++   L             W ++S+N F   +V
Sbjct: 242 DFWNPSSNDWHLHINRPLRDHEKNLWHNIKASLPTPLPNRGLPKPLWKLNSNNIFDTASV 301

Query: 343 KSLMGDMVGDSDPTSSKLYNVVWKDVYPKKIKIFIWELSLGAINTSDRLQRRMPYLHLSP 344
           K ++ +           LY  +WK  +PKK K FIW L  G INT+DRLQ+R+P   LSP
Sbjct: 302 KKILSEAPVSPANFHPNLYKTLWKVEFPKKCKFFIWTLIHGCINTADRLQKRLPNWALSP 361

[Close]

BLAST of Lag0007527 vs. NCBI nr
Match: KAA0039966.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 192.2 bits (487), Expect = 7.4e-45
Identity = 126/349 (36.10%), Postives = 180/349 (51.58%), Query Frame = 0


Query: 28  WVLQAKGFGLIWRKWICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDC 87
           ++L  KG+   WRKWI  CISSV YSIIINGRPR KI PSRGIRQGDP+S F+F+L  D 
Sbjct: 402 FMLMKKGYPFRWRKWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMDY 461

Query: 88  LNRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAF 147
           ++RLL+    +G  I        ++LTHL FADD LLF   D  ++ NL  II LF++A 
Sbjct: 462 ISRLLN---SVGEKIKGVKMEGNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLAS 521

Query: 148 GLNINFAKSELLGIHIVDSDMECLTAKFG----YLVASRIQRRLGNGCST-LFWHD---- 207
           GL+IN  KS +  I++  +  E + +++G    +L  + +   LG   +T  FW +    
Sbjct: 522 GLSINLNKSTISPINVGAARTEQIASQWGISTKFLPINYLGVPLGGKQTTKSFWKNVEEK 581

Query: 208 ------SW----LSCGVLS----------EAFPRLYRLSSRSEGTVADFWVSLNSAWDLS 267
                 SW    LS G               F  ++   S+ + ++ D W +    WDL+
Sbjct: 582 INKKLTSWKYSMLSKGAADPHGSPYAKDWTGFKDMFPGKSKMDSSIRDMWNNTLMDWDLN 641

Query: 268 LRRNLNDSETNEWASLSYLLSFIRIRVIDDTWSWPIDSSNAFTV----KSLMGDMVGDSD 327
            RR + D E   WA L   L+        D+ +W ++S   +TV    K+L        D
Sbjct: 642 PRRQIRDWEYPLWAELKNSLNVSFCENGKDSPTWALNSDGFYTVASVKKALQQPDQSFLD 701

Query: 328 PTSSKLYNVVWKDVYPKKIKIFIWELSLGAINTSDRLQRRMPYLHLSPS 344
             S   +  +WK   PKK   FIW L   ++NT+++L +RMP L   PS
Sbjct: 702 LQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLTKRMPNLCSRPS 747

[Close]

BLAST of Lag0007527 vs. NCBI nr
Match: TYK24536.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 192.2 bits (487), Expect = 7.4e-45
Identity = 126/349 (36.10%), Postives = 180/349 (51.58%), Query Frame = 0


Query: 28  WVLQAKGFGLIWRKWICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDC 87
           ++L  KG+   WRKWI  CISSV YSIIINGRPR KI PSRGIRQGDP+S F+F+L  D 
Sbjct: 503 FMLMKKGYPFRWRKWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMDY 562

Query: 88  LNRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAF 147
           ++RLL+    +G  I        ++LTHL FADD LLF   D  ++ NL  II LF++A 
Sbjct: 563 ISRLLN---SVGEKIKGVKMEGNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLAS 622

Query: 148 GLNINFAKSELLGIHIVDSDMECLTAKFG----YLVASRIQRRLGNGCST-LFWHD---- 207
           GL+IN  KS +  I++  +  E + +++G    +L  + +   LG   +T  FW +    
Sbjct: 623 GLSINLNKSTISPINVGAARTEQIASQWGISTKFLPINYLGVPLGGKQTTKSFWKNVEEK 682

Query: 208 ------SW----LSCGVLS----------EAFPRLYRLSSRSEGTVADFWVSLNSAWDLS 267
                 SW    LS G               F  ++   S+ + ++ D W +    WDL+
Sbjct: 683 INKKLTSWKYSMLSKGAADPHGSPYAKDWTGFKDMFPGKSKMDSSIRDMWNNTLMDWDLN 742

Query: 268 LRRNLNDSETNEWASLSYLLSFIRIRVIDDTWSWPIDSSNAFTV----KSLMGDMVGDSD 327
            RR + D E   WA L   L+        D+ +W ++S   +TV    K+L        D
Sbjct: 743 PRRQIRDWEYPLWAELKNSLNVSFCENGKDSPTWALNSDGFYTVASVKKALQQPDQSFLD 802

Query: 328 PTSSKLYNVVWKDVYPKKIKIFIWELSLGAINTSDRLQRRMPYLHLSPS 344
             S   +  +WK   PKK   FIW L   ++NT+++L +RMP L   PS
Sbjct: 803 LQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLTKRMPNLCSRPS 848

[Close]

BLAST of Lag0007527 vs. NCBI nr
Match: RVW38475.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 184.5 bits (467), Expect = 1.5e-42
Identity = 120/372 (32.26%), Postives = 175/372 (47.04%), Query Frame = 0


Query: 29  VLQAKGFGLIWRKWICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDCL 88
           VL+ KGF   WRKW+ GC+SSV+Y+I++NG  +  +  SRG+RQGDPLS FLF LV+D L
Sbjct: 381 VLEKKGFSPRWRKWMSGCLSSVSYAILVNGSAKGWVKASRGLRQGDPLSPFLFTLVADVL 440

Query: 89  NRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAFG 148
           +R+L  +     +    +G +R  ++HLQFADDT+ FS    + L  L  ++ +F    G
Sbjct: 441 SRMLMRAEERNMMEGFRVGRNRTRVSHLQFADDTIFFSNSREEELQTLKSLLLVFGHISG 500

Query: 149 LNINFAKSELLGIHIVDSDMECLTAKFG---------YL----------------VASRI 208
           L +N  KS + GI++  + +  L              YL                V  RI
Sbjct: 501 LKVNLNKSSIYGINLDQAHLSRLVVMLDCKASGWPILYLGLPLGGNPKACGFWDPVIERI 560

Query: 209 QRR------------------------LGNGCSTLFWHDSWLSCGVLSEAFPRLYRLSSR 268
             R                        +GNG    FW D W     L   +PRL+R+   
Sbjct: 561 SIRWSHQCPWKAIAQVFQGFSLITRYVVGNGERIRFWEDLWWGNQPLGTQYPRLFRV--- 620

Query: 269 SEGTVADFWVSLNS--------AWDLSLRRNLNDSETNEWASLSYLLSFIRIR-VIDDTW 328
               V D  +S+ S        +W+L+ RRNL++SE  +   L   L  + +   + D  
Sbjct: 621 ----VVDKNISIYSVLGPSRPFSWNLNFRRNLSNSEIEDLEGLMRSLDDLYLSPSVPDAR 680

Query: 329 SWPIDSSNAFTVKSLMGDMVGDSDPTSSKLYNVVWKDVYPKKIKIFIWELSLGAINTSDR 343
            WP+ SS  F+VKS    +   S    +     VW    P K+K F+W ++   +NT+D 
Sbjct: 681 LWPLSSSGLFSVKSFFLTLSQSSGSPQNFPSKFVWNSQVPFKVKSFVWLVAHKKVNTNDM 740

[Close]

BLAST of Lag0007527 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 6.1e-10
Identity = 32/68 (47.06%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 55  IINGRPREKIIPSRGIRQGDPLSLFLFILVSDCLNRLLSHSARLGRIISHPIGNSRLSLT 114
           IING P+  + PSRG+RQGDPLS +LFIL ++ L+ L   +   GR+    + N+   + 
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 115 HLQFADDT 123
           HL FADDT
Sbjct: 73  HLLFADDT 80

[Close]

BLAST of Lag0007527 vs. ExPASy Swiss-Prot
Match: Q03274 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.4e-06
Identity = 56/199 (28.14%), Postives = 84/199 (42.21%), Query Frame = 0


Query: 20  HAGICAILWVLQAKGFGLIWRKWICGCISSVNYSIIIN-GRPREKIIPSRGIRQGDPLSL 79
           H+ IC     LQ  G       +I G +S    +I +  G    KI   RG++QGDPLS 
Sbjct: 153 HSSICR---ALQRLGIDEGTSNYITGSLSDSTTTIRVGPGSQTRKICIRRGVKQGDPLSP 212

Query: 80  FLFILVSDCLNRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFE 139
           FLF  V D L   L  +  +G      IG  ++ +  L FADD LL    D      L  
Sbjct: 213 FLFNAVLDELLCSLQSTPGIG----GTIGEEKIPV--LAFADDLLLLEDNDVLLPTTLAT 272

Query: 140 IIKLFEMAFGLNINFAKSELLGIHIVDSDMECLTAKFGYLVASRIQRRLGNGCSTLFWHD 199
           +   F +  G+++N  KS  + I +  S   C+     +L    +   + +   T  +  
Sbjct: 273 VANFFRLR-GMSLNAKKS--VSISVAASGGVCIPRTKPFLRVDNVLLPVTDRMQTFRYLG 332

Query: 200 SWLSCGVLSEAFPRLYRLS 218
            +   G+   A P +Y LS
Sbjct: 333 HFF--GLSGAAKPTVYNLS 337

[Close]

BLAST of Lag0007527 vs. ExPASy Swiss-Prot
Match: P16423 (Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.3e-04
Identity = 37/105 (35.24%), Postives = 53/105 (50.48%), Query Frame = 0


Query: 57  NGRPREKIIPSRGIRQGDPLSLFLFILVSDCLNRLLSHSARLGRIISHPIGNSRLSLTHL 116
           +G   E+ +P+RG++QGDPLS  LF LV D L R L   + +G  + + I N+       
Sbjct: 526 DGWSSEEFVPARGVKQGDPLSPILFNLVMDRLLRTL--PSEIGAKVGNAITNA------A 585

Query: 117 QFADDTLLFSVYDSKALDNLFEIIKLFEMAFGLNINFAKSELLGI 162
            FADD +LF+      L  L +    F    GL +N  K   +GI
Sbjct: 586 AFADDLVLFA-ETRMGLQVLLDKTLDFLSIVGLKLNADKCFTVGI 621

[Close]

BLAST of Lag0007527 vs. ExPASy Swiss-Prot
Match: Q05118 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 5.0e-04
Identity = 51/193 (26.42%), Postives = 80/193 (41.45%), Query Frame = 0


Query: 25  AILWVLQAKGFGLIWRKWICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILV 84
           AIL  ++A G     + +I   I+    +I++ GR   KI    G++QGDPLS  LF +V
Sbjct: 41  AILRAMRAFGIDDGMQDFIMSTITDAYTNIVVGGRTTNKIYIRNGVKQGDPLSPVLFNIV 100

Query: 85  SDCLNRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFE 144
            D L   L+       +           +  L FADD LL    D    ++L      F 
Sbjct: 101 LDELVTRLNDEQPGASM------TPACKIASLAFADDLLLLEDRDIDVPNSLATTCAYFR 160

Query: 145 MAFGLNINFAKSELLGIHIVDSDMECLTAKFGYLVASRIQRRLGNGCSTLFWHDSWLSCG 204
              G+ +N  K   +    V S      +K  + +  R  + LG   +  +   ++ S G
Sbjct: 161 TR-GMTLNPEKCASISAATV-SGRSVPRSKPSFTIDGRYIKPLGGINTFKYLGLTFSSTG 220

Query: 205 VLSEAFPRLYRLS 218
           V   A P +Y L+
Sbjct: 221 V---AKPTVYNLT 222

[Close]

BLAST of Lag0007527 vs. ExPASy TrEMBL
Match: A0A1S3BGD2 (uncharacterized protein LOC103489530 OS=Cucumis melo OX=3656 GN=LOC103489530 PE=4 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 7.2e-46
Identity = 131/409 (32.03%), Postives = 194/409 (47.43%), Query Frame = 0


Query: 28  WVLQAKGFGLIWRKWICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDC 87
           +VL+ K F  +WRKWI GCIS+V YS+I+NGRP+ +I  +RG+RQGDPLS FLF++  D 
Sbjct: 125 FVLEKKNFPNLWRKWIKGCISNVTYSVIVNGRPQGRIKANRGLRQGDPLSPFLFVIAMDY 184

Query: 88  LNRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAF 147
           L+RLLSH    G I    + NS  +++H+ FA D LLF   +   L+NL   + LFE A 
Sbjct: 185 LSRLLSHLESSGAIKGVSL-NSNCNISHILFAYDILLFIEDNDCFLNNLRMALSLFERAS 244

Query: 148 GLNINFAKSELLGIHI-VDSDMECLT------AKFGYLV--------------------- 207
           GL IN  KS L+ +++ ++   EC +      ++  YL+                     
Sbjct: 245 GLKINLLKSALVPVNVSLNRAKECASFWGNNGSEGSYLINWTKVFKSKEEGGLGISRLHV 304

Query: 208 ----------------ASRIQRR------------------------------------- 267
                            + + RR                                     
Sbjct: 305 TNKALLSKWLWCYLSEPNALWRRLIQCKYKGNYPGDIPSNISSSTSKAPWRSIIDNIDWF 364

Query: 268 -------LGNGCSTLFWHDSWLSCGVLSEAFPRLYRLSSRSEGTVADFWVSLNSAWDLSL 327
                  L NG    FW+ +W   G LS A+PRL+ L+   E +V D W + ++ W++  
Sbjct: 365 KSNQSWELNNGDQISFWYSNWSQEGHLSTAYPRLFALTLGKEISVKDAWNTFDNQWNIIF 424

Query: 328 RRNLNDSETNEWASLSYLLSFIRIRVIDDTWSWPIDSSNAFTV---KSLMGDMVGDS--D 344
           RR LND E  +WA +  +L   R        +W  DS+N+F++   K L+   +  S  D
Sbjct: 425 RRELNDRERCKWAKILEILPTPRSNRRSSKPTWTPDSNNSFSIASAKVLISRQLDQSPRD 484

[Close]

BLAST of Lag0007527 vs. ExPASy TrEMBL
Match: A0A5A7TSA7 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001840 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.2e-45
Identity = 128/361 (35.46%), Postives = 176/361 (48.75%), Query Frame = 0


Query: 43  ICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDCLNRLLSHSARLGRII 102
           I  CISSV YSI+INGRPR +I PSRGIRQGDPLS F+F+L  D L+RLL++ A   R I
Sbjct: 2   IASCISSVQYSILINGRPRGRIKPSRGIRQGDPLSPFIFVLAMDYLSRLLNNLAD-KRKI 61

Query: 103 SHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAFGLNINFAKSELLGIH 162
           +    +  L+LTH+ FADD L+F       + NL  I+ LFE A GLNIN +KS +  I+
Sbjct: 62  NGVNFSPNLNLTHILFADDILIFVEDRDDYVSNLKMILHLFESASGLNINLSKSTIFPIN 121

Query: 163 IVDSDMECLTAKFG---------YL----------------VASRIQRRL---------- 222
           +     + +   +G         YL                V  +IQ++L          
Sbjct: 122 VPTDPAKSIADSWGISKGHLPTSYLGMPLGGKPSSSNFWDNVLQKIQKKLSSWKYSQLSK 181

Query: 223 ----------------------GNGCSTLFWHDSWLSCGVLSEAFPRLYRLSSRSEGTVA 282
                                  +G    FW D+W     LS   PRL+ LS+  +G+V 
Sbjct: 182 GGRITLINSTLESLPIYQIWKVNDGEDISFWLDNWNGNAPLSLVVPRLFALSTNKKGSVK 241

Query: 283 DFWVSLNSAWDLSLRRNLNDSETNEWASLSYLLSFIRIRVIDDTWSWPIDSSNAF---TV 342
           DFW   ++ W L + R L D E N W ++   L             W ++S+N F   +V
Sbjct: 242 DFWNPSSNDWHLHINRPLRDHEKNLWHNIKASLPTPLPNRGLPKPLWKLNSNNIFDTASV 301

Query: 343 KSLMGDMVGDSDPTSSKLYNVVWKDVYPKKIKIFIWELSLGAINTSDRLQRRMPYLHLSP 344
           K ++ +           LY  +WK  +PKK K FIW L  G INT+DRLQ+R+P   LSP
Sbjct: 302 KKILSEAPVSPANFHPNLYKTLWKVEFPKKCKFFIWTLIHGCINTADRLQKRLPNWALSP 361

[Close]

BLAST of Lag0007527 vs. ExPASy TrEMBL
Match: A0A5D3DLM2 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00780 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 3.6e-45
Identity = 126/349 (36.10%), Postives = 180/349 (51.58%), Query Frame = 0


Query: 28  WVLQAKGFGLIWRKWICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDC 87
           ++L  KG+   WRKWI  CISSV YSIIINGRPR KI PSRGIRQGDP+S F+F+L  D 
Sbjct: 503 FMLMKKGYPFRWRKWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMDY 562

Query: 88  LNRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAF 147
           ++RLL+    +G  I        ++LTHL FADD LLF   D  ++ NL  II LF++A 
Sbjct: 563 ISRLLN---SVGEKIKGVKMEGNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLAS 622

Query: 148 GLNINFAKSELLGIHIVDSDMECLTAKFG----YLVASRIQRRLGNGCST-LFWHD---- 207
           GL+IN  KS +  I++  +  E + +++G    +L  + +   LG   +T  FW +    
Sbjct: 623 GLSINLNKSTISPINVGAARTEQIASQWGISTKFLPINYLGVPLGGKQTTKSFWKNVEEK 682

Query: 208 ------SW----LSCGVLS----------EAFPRLYRLSSRSEGTVADFWVSLNSAWDLS 267
                 SW    LS G               F  ++   S+ + ++ D W +    WDL+
Sbjct: 683 INKKLTSWKYSMLSKGAADPHGSPYAKDWTGFKDMFPGKSKMDSSIRDMWNNTLMDWDLN 742

Query: 268 LRRNLNDSETNEWASLSYLLSFIRIRVIDDTWSWPIDSSNAFTV----KSLMGDMVGDSD 327
            RR + D E   WA L   L+        D+ +W ++S   +TV    K+L        D
Sbjct: 743 PRRQIRDWEYPLWAELKNSLNVSFCENGKDSPTWALNSDGFYTVASVKKALQQPDQSFLD 802

Query: 328 PTSSKLYNVVWKDVYPKKIKIFIWELSLGAINTSDRLQRRMPYLHLSPS 344
             S   +  +WK   PKK   FIW L   ++NT+++L +RMP L   PS
Sbjct: 803 LQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLTKRMPNLCSRPS 848

[Close]

BLAST of Lag0007527 vs. ExPASy TrEMBL
Match: A0A5A7TCY9 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G002480 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 3.6e-45
Identity = 126/349 (36.10%), Postives = 180/349 (51.58%), Query Frame = 0


Query: 28  WVLQAKGFGLIWRKWICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDC 87
           ++L  KG+   WRKWI  CISSV YSIIINGRPR KI PSRGIRQGDP+S F+F+L  D 
Sbjct: 402 FMLMKKGYPFRWRKWIRACISSVQYSIIINGRPRGKIQPSRGIRQGDPISPFIFVLAMDY 461

Query: 88  LNRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAF 147
           ++RLL+    +G  I        ++LTHL FADD LLF   D  ++ NL  II LF++A 
Sbjct: 462 ISRLLN---SVGEKIKGVKMEGNINLTHLLFADDILLFVEDDEHSIQNLKNIINLFQLAS 521

Query: 148 GLNINFAKSELLGIHIVDSDMECLTAKFG----YLVASRIQRRLGNGCST-LFWHD---- 207
           GL+IN  KS +  I++  +  E + +++G    +L  + +   LG   +T  FW +    
Sbjct: 522 GLSINLNKSTISPINVGAARTEQIASQWGISTKFLPINYLGVPLGGKQTTKSFWKNVEEK 581

Query: 208 ------SW----LSCGVLS----------EAFPRLYRLSSRSEGTVADFWVSLNSAWDLS 267
                 SW    LS G               F  ++   S+ + ++ D W +    WDL+
Sbjct: 582 INKKLTSWKYSMLSKGAADPHGSPYAKDWTGFKDMFPGKSKMDSSIRDMWNNTLMDWDLN 641

Query: 268 LRRNLNDSETNEWASLSYLLSFIRIRVIDDTWSWPIDSSNAFTV----KSLMGDMVGDSD 327
            RR + D E   WA L   L+        D+ +W ++S   +TV    K+L        D
Sbjct: 642 PRRQIRDWEYPLWAELKNSLNVSFCENGKDSPTWALNSDGFYTVASVKKALQQPDQSFLD 701

Query: 328 PTSSKLYNVVWKDVYPKKIKIFIWELSLGAINTSDRLQRRMPYLHLSPS 344
             S   +  +WK   PKK   FIW L   ++NT+++L +RMP L   PS
Sbjct: 702 LQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLTKRMPNLCSRPS 747

[Close]

BLAST of Lag0007527 vs. ExPASy TrEMBL
Match: A0A438DSJ8 (Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX2_255 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 7.5e-43
Identity = 120/372 (32.26%), Postives = 175/372 (47.04%), Query Frame = 0


Query: 29  VLQAKGFGLIWRKWICGCISSVNYSIIINGRPREKIIPSRGIRQGDPLSLFLFILVSDCL 88
           VL+ KGF   WRKW+ GC+SSV+Y+I++NG  +  +  SRG+RQGDPLS FLF LV+D L
Sbjct: 381 VLEKKGFSPRWRKWMSGCLSSVSYAILVNGSAKGWVKASRGLRQGDPLSPFLFTLVADVL 440

Query: 89  NRLLSHSARLGRIISHPIGNSRLSLTHLQFADDTLLFSVYDSKALDNLFEIIKLFEMAFG 148
           +R+L  +     +    +G +R  ++HLQFADDT+ FS    + L  L  ++ +F    G
Sbjct: 441 SRMLMRAEERNMMEGFRVGRNRTRVSHLQFADDTIFFSNSREEELQTLKSLLLVFGHISG 500

Query: 149 LNINFAKSELLGIHIVDSDMECLTAKFG---------YL----------------VASRI 208
           L +N  KS + GI++  + +  L              YL                V  RI
Sbjct: 501 LKVNLNKSSIYGINLDQAHLSRLVVMLDCKASGWPILYLGLPLGGNPKACGFWDPVIERI 560

Query: 209 QRR------------------------LGNGCSTLFWHDSWLSCGVLSEAFPRLYRLSSR 268
             R                        +GNG    FW D W     L   +PRL+R+   
Sbjct: 561 SIRWSHQCPWKAIAQVFQGFSLITRYVVGNGERIRFWEDLWWGNQPLGTQYPRLFRV--- 620

Query: 269 SEGTVADFWVSLNS--------AWDLSLRRNLNDSETNEWASLSYLLSFIRIR-VIDDTW 328
               V D  +S+ S        +W+L+ RRNL++SE  +   L   L  + +   + D  
Sbjct: 621 ----VVDKNISIYSVLGPSRPFSWNLNFRRNLSNSEIEDLEGLMRSLDDLYLSPSVPDAR 680

Query: 329 SWPIDSSNAFTVKSLMGDMVGDSDPTSSKLYNVVWKDVYPKKIKIFIWELSLGAINTSDR 343
            WP+ SS  F+VKS    +   S    +     VW    P K+K F+W ++   +NT+D 
Sbjct: 681 LWPLSSSGLFSVKSFFLTLSQSSGSPQNFPSKFVWNSQVPFKVKSFVWLVAHKKVNTNDM 740

[Close]

BLAST of Lag0007527 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 66.6 bits (161), Expect = 4.4e-11
Identity = 32/68 (47.06%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 55  IINGRPREKIIPSRGIRQGDPLSLFLFILVSDCLNRLLSHSARLGRIISHPIGNSRLSLT 114
           IING P+  + PSRG+RQGDPLS +LFIL ++ L+ L   +   GR+    + N+   + 
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 115 HLQFADDT 123
           HL FADDT
Sbjct: 73  HLLFADDT 80

The following BLAST results are available for this feature:

BLAST of Lag0007527 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446987.1	1.5e-45	32.03	PREDICTED: uncharacterized protein LOC103489530 [Cucumis melo]	[more]
KAA0044451.1	2.5e-45	35.46	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]	[more]
KAA0039966.1	7.4e-45	36.10	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]	[more]
TYK24536.1	7.4e-45	36.10	LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]	[more]
RVW38475.1	1.5e-42	32.26	Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera]	[more]

BLAST of Lag0007527 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P92555	6.1e-10	47.06	Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q03274	1.4e-06	28.14	Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra...	[more]
P16423	2.3e-04	35.24	Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS...	[more]
Q05118	5.0e-04	26.42	Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fra...	[more]

BLAST of Lag0007527 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BGD2	7.2e-46	32.03	uncharacterized protein LOC103489530 OS=Cucumis melo OX=3656 GN=LOC103489530 PE=...	[more]
A0A5A7TSA7	1.2e-45	35.46	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5D3DLM2	3.6e-45	36.10	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A5A7TCY9	3.6e-45	36.10	LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119...	[more]
A0A438DSJ8	7.5e-43	32.26	Transposon TX1 uncharacterized 149 kDa protein OS=Vitis vinifera OX=29760 GN=YTX...	[more]

BLAST of Lag0007527 vs. TAIR 10


Match Name	E-value	Identity	Description
ATMG01250.1	4.4e-11	47.06	RNA-directed DNA polymerase (reverse transcriptase)	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000477	Reverse transcriptase domain	PFAM	PF00078	RVT_1	coord: 36..161 e-value: 2.2E-13 score: 50.3
IPR000477	Reverse transcriptase domain	PROSITE	PS50878	RT_POL	coord: 1..179 score: 10.536036
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 28..175
None	No IPR available	PANTHER	PTHR33116:SF38	OS01G0158850 PROTEIN	coord: 28..175
None	No IPR available	PANTHER	PTHR33116	REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATED	coord: 183..334
None	No IPR available	PANTHER	PTHR33116:SF38	OS01G0158850 PROTEIN	coord: 183..334

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0007527.1	Lag0007527.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009987	cellular process