Sed0000514 (gene) Chayote v1

Overview


Name	Sed0000514
Type	gene
Organism	Sechium edule (Chayote v1)
Description	Unknown protein
Location	LG04: 20453543 .. 20454519 (-)
RNA-Seq Expression	Sed0000514
Synteny	Sed0000514

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTGAAATTTTCTCTCCTCTCTACACAGCTTTTTTTATTTTTTTTATCAATCTCCATCAGCAGCCAACACAACTCGAAGATCCCAAATAAAAGGAAATCCCAGAATTTGAAGCTTTTGAAAAATTTCTCTCAAACCATCTTTCAGTTTGTCGCAATTCTATACACACTGCACAAATCTGGGTTGTGATTCTAAGCTGTTGTTTTGATTTTGATTGTAATTTTGATTTTGCTTTGTGCGACTAAAAATTGAATTCTGGGAGAATGAAATTTGCAAGAGCAGTTTCTGAAATTCCAAAGCCCAGAGGGTAGAGAATAATAATTATTATCCTGATTGCTTGGGTTCCAATTCAATGGCGGTGCACAGAGACTGGTTTCTGGCATTGTTCTTGTGTTACGCAGTCGTTTCGGAGGTAACCAATGCTTCGGTTTGGGATTCAAGAAAGTTGCTAGACCCGGCTTCTAAAGTTAACTCCACCGCACCAGTGAGTAACCAACCATCTACTTTCCTTCAATTAATGGAACAATCTCATATTTCTCTGTACTATTCTTTTATGAACCTTTTGTTTTTCCCCTTTTAGGTTTCCCCTCTCATCAGTCCTGTTCCTTCCATGAATGATTCGAATTCTTCTGTACCCGTTGATGCAAACAAGAAGCAAATGGAACCACCCCTGCCTGCGGTTGCACCCAACAATTCATCTTCCAAAGTCGATTTGAAAATACTCAATAATTCGGGTTCACCTAATGTAAGCGAGACTGGCAATGAAGAAAAGCCTTTGGAGACGAAGAAGGAGAACGGGACTGAAGCCGGTCCTCAATCAAGTAATGGTGAAACTTGTGATGGGGTACCCGACAATAAGAAGTGCAGGGACCACAACAAGTTGGTTGCTTGTTTCCAAAACAATCTTATTGGTAAATATGAATTGCTCTTCGGCCTTATTACCCTTTTAAGCTTCATTTTTTTCTGGCGTGGATTCTAA

mRNA sequence

GCTGAAATTTTCTCTCCTCTCTACACAGCTTTTTTTATTTTTTTTATCAATCTCCATCAGCAGCCAACACAACTCGAAGATCCCAAATAAAAGGAAATCCCAGAATTTGAAGCTTTTGAAAAATTTCTCTCAAACCATCTTTCAGTTTGTCGCAATTCTATACACACTGCACAAATCTGGGTTGTGATTCTAAGCTGTTGTTTTGATTTTGATTGTAATTTTGATTTTGCTTTGTGCGACTAAAAATTGAATTCTGGGAGAATGAAATTTGCAAGAGCAGTTTCTGAAATTCCAAAGCCCAGAGGGTAGAGAATAATAATTATTATCCTGATTGCTTGGGTTCCAATTCAATGGCGGTGCACAGAGACTGGTTTCTGGCATTGTTCTTGTGTTACGCAGTCGTTTCGGAGGTAACCAATGCTTCGGTTTGGGATTCAAGAAAGTTGCTAGACCCGGCTTCTAAAGTTAACTCCACCGCACCAGTTTCCCCTCTCATCAGTCCTGTTCCTTCCATGAATGATTCGAATTCTTCTGTACCCGTTGATGCAAACAAGAAGCAAATGGAACCACCCCTGCCTGCGGTTGCACCCAACAATTCATCTTCCAAAGTCGATTTGAAAATACTCAATAATTCGGGTTCACCTAATGTAAGCGAGACTGGCAATGAAGAAAAGCCTTTGGAGACGAAGAAGGAGAACGGGACTGAAGCCGGTCCTCAATCAAGTAATGGTGAAACTTGTGATGGGGTACCCGACAATAAGAAGTGCAGGGACCACAACAAGTTGGTTGCTTGTTTCCAAAACAATCTTATTGGTAAATATGAATTGCTCTTCGGCCTTATTACCCTTTTAAGCTTCATTTTTTTCTGGCGTGGATTCTAA

Coding sequence (CDS)

ATGGCGGTGCACAGAGACTGGTTTCTGGCATTGTTCTTGTGTTACGCAGTCGTTTCGGAGGTAACCAATGCTTCGGTTTGGGATTCAAGAAAGTTGCTAGACCCGGCTTCTAAAGTTAACTCCACCGCACCAGTTTCCCCTCTCATCAGTCCTGTTCCTTCCATGAATGATTCGAATTCTTCTGTACCCGTTGATGCAAACAAGAAGCAAATGGAACCACCCCTGCCTGCGGTTGCACCCAACAATTCATCTTCCAAAGTCGATTTGAAAATACTCAATAATTCGGGTTCACCTAATGTAAGCGAGACTGGCAATGAAGAAAAGCCTTTGGAGACGAAGAAGGAGAACGGGACTGAAGCCGGTCCTCAATCAAGTAATGGTGAAACTTGTGATGGGGTACCCGACAATAAGAAGTGCAGGGACCACAACAAGTTGGTTGCTTGTTTCCAAAACAATCTTATTGGTAAATATGAATTGCTCTTCGGCCTTATTACCCTTTTAAGCTTCATTTTTTTCTGGCGTGGATTCTAA

Protein sequence

MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVNSTAPVSPLISPVPSMNDSNSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNNSGSPNVSETGNEEKPLETKKENGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLIGKYELLFGLITLLSFIFFWRGF

Homology

[Close]

BLAST of Sed0000514 vs. NCBI nr
Match: XP_038904076.1 (uncharacterized protein LOC120090465 [Benincasa hispida])

HSP 1 Score: 184.9 bits (468), Expect = 6.1e-43
Identity = 110/159 (69.18%), Postives = 123/159 (77.36%), Query Frame = 0


Query: 1   MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVNS--TAPVSPLISPVPSMNDS 60
           MA+ RD FLALF CYAVVSEVTNA+ WDSRKLL+PAS  NS  T+P SPLISPVPS+N+S
Sbjct: 1   MAIRRDCFLALFFCYAVVSEVTNATQWDSRKLLEPASNHNSTRTSPGSPLISPVPSVNNS 60

Query: 61  NSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNN---SGSPNVSETGNEEKPLETKKE 120
           + SVPV+  KK  EPPLP  APN SSS VD K+LNN   S SP +SETG+  KP +TKKE
Sbjct: 61  D-SVPVNGKKKHNEPPLP--APNKSSSNVDTKLLNNSSSSSSPAISETGS-RKPSDTKKE 120

Query: 121 NGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLI 155
           NGTE    SSN ETCDGVPDN KCRD  KLVAC Q+NLI
Sbjct: 121 NGTE----SSNRETCDGVPDNNKCRDQKKLVACIQSNLI 151

[Close]

BLAST of Sed0000514 vs. NCBI nr
Match: KAG7027858.1 (hypothetical protein SDJN02_09035 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 178.3 bits (451), Expect = 5.7e-41
Identity = 112/164 (68.29%), Postives = 121/164 (73.78%), Query Frame = 0


Query: 1   MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVNS--TAPVSPLISPVPSMNDS 60
           MA+  D FLALF CYAVVSEVTNAS+WDSRKLLDPASK NS  T+PV PLISP  S N S
Sbjct: 1   MAMRGDCFLALFFCYAVVSEVTNASLWDSRKLLDPASKDNSTGTSPVLPLISPNSSTNHS 60

Query: 61  NSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNNS---GSPNVSETGNEEKPLETKKE 120
           +S+ PVD  KKQ EPP P  APNNSSS +D K LNNS    S NVSE GN  KPL+T K 
Sbjct: 61  DSA-PVD-GKKQREPPPP--APNNSSSNLDSKGLNNSSSGSSHNVSEAGN-GKPLDTNKG 120

Query: 121 NGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLIGKYEL 160
           N TE G QSSN E CDGVPDNKKCRD  KLVAC QNN+I   +L
Sbjct: 121 NRTEGGLQSSNREICDGVPDNKKCRDQKKLVACIQNNIIESKDL 159

[Close]

BLAST of Sed0000514 vs. NCBI nr
Match: XP_022935129.1 (uncharacterized protein LOC111442092 [Cucurbita moschata])

HSP 1 Score: 177.2 bits (448), Expect = 1.3e-40
Identity = 104/159 (65.41%), Postives = 124/159 (77.99%), Query Frame = 0


Query: 1   MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVNS--TAPVSPLISPVPSMNDS 60
           MA+ RD FLALFLC+AVVSEVTNAS+WDSRKLL+PAS  NS  T+  SP+ISP+ S+N S
Sbjct: 1   MAMRRDCFLALFLCHAVVSEVTNASLWDSRKLLEPASNNNSTRTSQGSPVISPISSVNIS 60

Query: 61  NSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNNS---GSPNVSETGNEEKPLETKKE 120
           +S+ PV   KKQ+EPP P  +PNNSSS ++  +LNNS    S  VSETG+  KP++TKKE
Sbjct: 61  DSA-PVIGKKKQVEPPPP--SPNNSSSSLESNVLNNSSSRSSSTVSETGS-GKPIDTKKE 120

Query: 121 NGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLI 155
           NGTEA   SS  ETCDGVPDNKKCRD NKL+AC Q+NLI
Sbjct: 121 NGTEASALSSKHETCDGVPDNKKCRDQNKLIACIQSNLI 155

[Close]

BLAST of Sed0000514 vs. NCBI nr
Match: XP_022971211.1 (uncharacterized protein LOC111470002 [Cucurbita maxima])

HSP 1 Score: 176.8 bits (447), Expect = 1.6e-40
Identity = 110/164 (67.07%), Postives = 122/164 (74.39%), Query Frame = 0


Query: 1   MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVNS--TAPVSPLISPVPSMNDS 60
           MA+  D FLALF CYAVVSEVTNAS+WDSRKLLDPASK NS  T+PV PLISP  SMN S
Sbjct: 1   MAMRGDCFLALFFCYAVVSEVTNASLWDSRKLLDPASKDNSTGTSPVLPLISPNSSMNHS 60

Query: 61  NSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNNS---GSPNVSETGNEEKPLETKKE 120
           +S+ PVD  KK+ EPP P  APNNSSS +D K LNNS    S NVSE GN  KPL+T K 
Sbjct: 61  DSA-PVD-GKKRREPPPP--APNNSSSNLDSKGLNNSSSGSSHNVSEAGN-GKPLDTNKR 120

Query: 121 NGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLIGKYEL 160
           N TE G QSSN E CDGVPDNKKCRD  KLVAC ++N+I   +L
Sbjct: 121 NRTEGGLQSSNREICDGVPDNKKCRDQKKLVACIESNIIESKDL 159

[Close]

BLAST of Sed0000514 vs. NCBI nr
Match: XP_022941631.1 (uncharacterized protein LOC111446930 [Cucurbita moschata])

HSP 1 Score: 176.4 bits (446), Expect = 2.2e-40
Identity = 111/164 (67.68%), Postives = 120/164 (73.17%), Query Frame = 0


Query: 1   MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVNS--TAPVSPLISPVPSMNDS 60
           MA+  D FLALF CYAVVSEVTNAS+WDSRKLLDPASK NS  T+PV PLISP  S N S
Sbjct: 1   MAMRGDCFLALFFCYAVVSEVTNASLWDSRKLLDPASKDNSTGTSPVLPLISPNSSTNHS 60

Query: 61  NSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNNS---GSPNVSETGNEEKPLETKKE 120
           +S+ PVD  KKQ EPP P  APNNSSS +D K LNNS    S NVSE GN  KPL+T   
Sbjct: 61  DSA-PVD-GKKQREPPPP--APNNSSSNLDSKGLNNSSSGSSHNVSEAGN-GKPLDTNNG 120

Query: 121 NGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLIGKYEL 160
           N TE G QSSN E CDGVPDNKKCRD  KLVAC QNN+I   +L
Sbjct: 121 NRTEGGLQSSNHEICDGVPDNKKCRDQKKLVACIQNNIIESKDL 159

[Close]

BLAST of Sed0000514 vs. ExPASy TrEMBL
Match: A0A6J1F4I5 (uncharacterized protein LOC111442092 OS=Cucurbita moschata OX=3662 GN=LOC111442092 PE=4 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 6.1e-41
Identity = 104/159 (65.41%), Postives = 124/159 (77.99%), Query Frame = 0


Query: 1   MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVNS--TAPVSPLISPVPSMNDS 60
           MA+ RD FLALFLC+AVVSEVTNAS+WDSRKLL+PAS  NS  T+  SP+ISP+ S+N S
Sbjct: 1   MAMRRDCFLALFLCHAVVSEVTNASLWDSRKLLEPASNNNSTRTSQGSPVISPISSVNIS 60

Query: 61  NSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNNS---GSPNVSETGNEEKPLETKKE 120
           +S+ PV   KKQ+EPP P  +PNNSSS ++  +LNNS    S  VSETG+  KP++TKKE
Sbjct: 61  DSA-PVIGKKKQVEPPPP--SPNNSSSSLESNVLNNSSSRSSSTVSETGS-GKPIDTKKE 120

Query: 121 NGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLI 155
           NGTEA   SS  ETCDGVPDNKKCRD NKL+AC Q+NLI
Sbjct: 121 NGTEASALSSKHETCDGVPDNKKCRDQNKLIACIQSNLI 155

[Close]

BLAST of Sed0000514 vs. ExPASy TrEMBL
Match: A0A6J1I2Q4 (uncharacterized protein LOC111470002 OS=Cucurbita maxima OX=3661 GN=LOC111470002 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 8.0e-41
Identity = 110/164 (67.07%), Postives = 122/164 (74.39%), Query Frame = 0


Query: 1   MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVNS--TAPVSPLISPVPSMNDS 60
           MA+  D FLALF CYAVVSEVTNAS+WDSRKLLDPASK NS  T+PV PLISP  SMN S
Sbjct: 1   MAMRGDCFLALFFCYAVVSEVTNASLWDSRKLLDPASKDNSTGTSPVLPLISPNSSMNHS 60

Query: 61  NSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNNS---GSPNVSETGNEEKPLETKKE 120
           +S+ PVD  KK+ EPP P  APNNSSS +D K LNNS    S NVSE GN  KPL+T K 
Sbjct: 61  DSA-PVD-GKKRREPPPP--APNNSSSNLDSKGLNNSSSGSSHNVSEAGN-GKPLDTNKR 120

Query: 121 NGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLIGKYEL 160
           N TE G QSSN E CDGVPDNKKCRD  KLVAC ++N+I   +L
Sbjct: 121 NRTEGGLQSSNREICDGVPDNKKCRDQKKLVACIESNIIESKDL 159

[Close]

BLAST of Sed0000514 vs. ExPASy TrEMBL
Match: A0A6J1FLM5 (uncharacterized protein LOC111446930 OS=Cucurbita moschata OX=3662 GN=LOC111446930 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 1.0e-40
Identity = 111/164 (67.68%), Postives = 120/164 (73.17%), Query Frame = 0


Query: 1   MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVNS--TAPVSPLISPVPSMNDS 60
           MA+  D FLALF CYAVVSEVTNAS+WDSRKLLDPASK NS  T+PV PLISP  S N S
Sbjct: 1   MAMRGDCFLALFFCYAVVSEVTNASLWDSRKLLDPASKDNSTGTSPVLPLISPNSSTNHS 60

Query: 61  NSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNNS---GSPNVSETGNEEKPLETKKE 120
           +S+ PVD  KKQ EPP P  APNNSSS +D K LNNS    S NVSE GN  KPL+T   
Sbjct: 61  DSA-PVD-GKKQREPPPP--APNNSSSNLDSKGLNNSSSGSSHNVSEAGN-GKPLDTNNG 120

Query: 121 NGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLIGKYEL 160
           N TE G QSSN E CDGVPDNKKCRD  KLVAC QNN+I   +L
Sbjct: 121 NRTEGGLQSSNHEICDGVPDNKKCRDQKKLVACIQNNIIESKDL 159

[Close]

BLAST of Sed0000514 vs. ExPASy TrEMBL
Match: A0A6J1DSQ9 (uncharacterized protein LOC111024051 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111024051 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 1.5e-39
Identity = 104/165 (63.03%), Postives = 122/165 (73.94%), Query Frame = 0


Query: 1   MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVN--STAPVSPLISPVPSMNDS 60
           MA+ RDWFLALF  YAVVSEVT+AS+WDSRKLLDPA K N  +T+ VSP+I+P  S+N+S
Sbjct: 1   MAMRRDWFLALFFAYAVVSEVTHASLWDSRKLLDPAPKDNTTTTSAVSPVINPASSVNNS 60

Query: 61  NSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNNSGS----PNVSETGNEEKPLETKK 120
           + SVPV+  KK  EPP PA+  NNS S VD K+L+NS S    P VSETGN  KP  TKK
Sbjct: 61  D-SVPVN-GKKHTEPPPPAL--NNSLSNVDSKVLSNSSSGSPPPTVSETGN-GKPFVTKK 120

Query: 121 ENGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLIGKYEL 160
            N TEAGPQSSN E CDG+ D KKCRD  KLVAC ++NLI   +L
Sbjct: 121 NNDTEAGPQSSNRENCDGIADKKKCRDQKKLVACIKSNLIESRDL 160

[Close]

BLAST of Sed0000514 vs. ExPASy TrEMBL
Match: A0A6J1DXQ4 (uncharacterized protein LOC111024051 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111024051 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 3.4e-39
Identity = 103/160 (64.38%), Postives = 120/160 (75.00%), Query Frame = 0


Query: 1   MAVHRDWFLALFLCYAVVSEVTNASVWDSRKLLDPASKVN--STAPVSPLISPVPSMNDS 60
           MA+ RDWFLALF  YAVVSEVT+AS+WDSRKLLDPA K N  +T+ VSP+I+P  S+N+S
Sbjct: 1   MAMRRDWFLALFFAYAVVSEVTHASLWDSRKLLDPAPKDNTTTTSAVSPVINPASSVNNS 60

Query: 61  NSSVPVDANKKQMEPPLPAVAPNNSSSKVDLKILNNSGS----PNVSETGNEEKPLETKK 120
           + SVPV+  KK  EPP PA+  NNS S VD K+L+NS S    P VSETGN  KP  TKK
Sbjct: 61  D-SVPVN-GKKHTEPPPPAL--NNSLSNVDSKVLSNSSSGSPPPTVSETGN-GKPFVTKK 120

Query: 121 ENGTEAGPQSSNGETCDGVPDNKKCRDHNKLVACFQNNLI 155
            N TEAGPQSSN E CDG+ D KKCRD  KLVAC ++NLI
Sbjct: 121 NNDTEAGPQSSNRENCDGIADKKKCRDQKKLVACIKSNLI 155

The following BLAST results are available for this feature:

BLAST of Sed0000514 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904076.1	6.1e-43	69.18	uncharacterized protein LOC120090465 [Benincasa hispida]	[more]
KAG7027858.1	5.7e-41	68.29	hypothetical protein SDJN02_09035 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022935129.1	1.3e-40	65.41	uncharacterized protein LOC111442092 [Cucurbita moschata]	[more]
XP_022971211.1	1.6e-40	67.07	uncharacterized protein LOC111470002 [Cucurbita maxima]	[more]
XP_022941631.1	2.2e-40	67.68	uncharacterized protein LOC111446930 [Cucurbita moschata]	[more]

BLAST of Sed0000514 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Sed0000514 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F4I5	6.1e-41	65.41	uncharacterized protein LOC111442092 OS=Cucurbita moschata OX=3662 GN=LOC1114420...	[more]
A0A6J1I2Q4	8.0e-41	67.07	uncharacterized protein LOC111470002 OS=Cucurbita maxima OX=3661 GN=LOC111470002...	[more]
A0A6J1FLM5	1.0e-40	67.68	uncharacterized protein LOC111446930 OS=Cucurbita moschata OX=3662 GN=LOC1114469...	[more]
A0A6J1DSQ9	1.5e-39	63.03	uncharacterized protein LOC111024051 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DXQ4	3.4e-39	64.38	uncharacterized protein LOC111024051 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Sed0000514 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 77..105
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..66
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 117..131
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sed0000514.1	Sed0000514.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane