Lag0000584 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0000584
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DNA-directed RNA polymerase subunit beta
Location	chr4: 10687995 .. 10688324 (-)
RNA-Seq Expression	Lag0000584
Synteny	Lag0000584

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTTATTTATCACCAAGTCGAGATGAATACTATATGGTAGCGACAGGAAATTCTTTGGCACTGAATCCAGGTATTCAGGAAGAACAGATTGTTCCAGCTCGATATCGTCAAGAATTCTTGACTATTGAATGGGAACAAGTTCATCTTCGAAGTATTTTTCCCTTCCAATATTTTTCTATTGGAGCTTCCCTCATTCCTTTTATCGAACATAATGATGCGAATCGTGCTTTAATGAGTTCTAATATGCAACGTCAAGCAGTCCCGCTTTCGTTGCAGCTTGCAAAACTGCAGGTGGTCCTCTCGTCCCGAATTGCTGAGAGTTCTTAG

mRNA sequence

ATGCTTTATTTATCACCAAGTCGAGATGAATACTATATGGTAGCGACAGGAAATTCTTTGGCACTGAATCCAGGTATTCAGGAAGAACAGATTGTTCCAGCTCGATATCGTCAAGAATTCTTGACTATTGAATGGGAACAAGTTCATCTTCGAAGTATTTTTCCCTTCCAATATTTTTCTATTGGAGCTTCCCTCATTCCTTTTATCGAACATAATGATGCGAATCGTGCTTTAATGAGTTCTAATATGCAACGTCAAGCAGTCCCGCTTTCGTTGCAGCTTGCAAAACTGCAGGTGGTCCTCTCGTCCCGAATTGCTGAGAGTTCTTAG

Coding sequence (CDS)

ATGCTTTATTTATCACCAAGTCGAGATGAATACTATATGGTAGCGACAGGAAATTCTTTGGCACTGAATCCAGGTATTCAGGAAGAACAGATTGTTCCAGCTCGATATCGTCAAGAATTCTTGACTATTGAATGGGAACAAGTTCATCTTCGAAGTATTTTTCCCTTCCAATATTTTTCTATTGGAGCTTCCCTCATTCCTTTTATCGAACATAATGATGCGAATCGTGCTTTAATGAGTTCTAATATGCAACGTCAAGCAGTCCCGCTTTCGTTGCAGCTTGCAAAACTGCAGGTGGTCCTCTCGTCCCGAATTGCTGAGAGTTCTTAG

Protein sequence

MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFSIGASLIPFIEHNDANRALMSSNMQRQAVPLSLQLAKLQVVLSSRIAESS

Homology

[Close]

BLAST of Lag0000584 vs. NCBI nr
Match: YP_009752827.1 (RNA polymerase subunit beta [Baijiania yunnanensis] >QIT05899.1 RNA polymerase subunit beta [Baijiania yunnanensis])

HSP 1 Score: 184.9 bits (468), Expect = 3.8e-43
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 1   MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 60
           MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS
Sbjct: 466 MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 525

Query: 61  IGASLIPFIEHNDANRALMSSNMQRQAVPLS 92
           IGASLIPFIEHNDANRALMSSNMQRQAVPLS
Sbjct: 526 IGASLIPFIEHNDANRALMSSNMQRQAVPLS 556

[Close]

BLAST of Lag0000584 vs. NCBI nr
Match: AHM88854.1 (RNA polymerase beta subunit, partial [Lagenaria siceraria])

HSP 1 Score: 184.9 bits (468), Expect = 3.8e-43
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 1   MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 60
           MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS
Sbjct: 454 MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 513

Query: 61  IGASLIPFIEHNDANRALMSSNMQRQAVPLS 92
           IGASLIPFIEHNDANRALMSSNMQRQAVPLS
Sbjct: 514 IGASLIPFIEHNDANRALMSSNMQRQAVPLS 544

[Close]

BLAST of Lag0000584 vs. NCBI nr
Match: QJR52948.1 (RNA polymerase beta subunit [Herpetospermum pedunculosum])

HSP 1 Score: 184.9 bits (468), Expect = 3.8e-43
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 1   MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 60
           MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS
Sbjct: 466 MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 525

Query: 61  IGASLIPFIEHNDANRALMSSNMQRQAVPLS 92
           IGASLIPFIEHNDANRALMSSNMQRQAVPLS
Sbjct: 526 IGASLIPFIEHNDANRALMSSNMQRQAVPLS 556

[Close]

BLAST of Lag0000584 vs. NCBI nr
Match: YP_009753697.1 (RNA polymerase subunit beta [Trichosanthes nervifolia] >QIT05308.1 RNA polymerase subunit beta [Trichosanthes nervifolia])

HSP 1 Score: 184.9 bits (468), Expect = 3.8e-43
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 1   MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 60
           MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS
Sbjct: 466 MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 525

Query: 61  IGASLIPFIEHNDANRALMSSNMQRQAVPLS 92
           IGASLIPFIEHNDANRALMSSNMQRQAVPLS
Sbjct: 526 IGASLIPFIEHNDANRALMSSNMQRQAVPLS 556

[Close]

BLAST of Lag0000584 vs. NCBI nr
Match: YP_009752742.1 (RNA polymerase subunit beta [Cyclantheropsis parviflora] >QIT05392.1 RNA polymerase subunit beta [Cyclantheropsis parviflora])

HSP 1 Score: 184.9 bits (468), Expect = 3.8e-43
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 1   MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 60
           MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS
Sbjct: 466 MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 525

Query: 61  IGASLIPFIEHNDANRALMSSNMQRQAVPLS 92
           IGASLIPFIEHNDANRALMSSNMQRQAVPLS
Sbjct: 526 IGASLIPFIEHNDANRALMSSNMQRQAVPLS 556

[Close]

BLAST of Lag0000584 vs. ExPASy Swiss-Prot
Match: Q4VZP1 (DNA-directed RNA polymerase subunit beta OS=Cucumis sativus OX=3659 GN=rpoB PE=3 SV=2)

HSP 1 Score: 184.9 bits (468), Expect = 4.9e-46
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0


Query: 1   MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 60
           MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS
Sbjct: 466 MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 525

Query: 61  IGASLIPFIEHNDANRALMSSNMQRQAVPLS 92
           IGASLIPFIEHNDANRALMSSNMQRQAVPLS
Sbjct: 526 IGASLIPFIEHNDANRALMSSNMQRQAVPLS 556

[Close]

BLAST of Lag0000584 vs. ExPASy Swiss-Prot
Match: A0ZZ27 (DNA-directed RNA polymerase subunit beta OS=Gossypium barbadense OX=3634 GN=rpoB PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.0e-43
Identity = 87/91 (95.60%), Postives = 88/91 (96.70%), Query Frame = 0


Query: 1   MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 60
           MLYLSPSRDEYYMVA GNSLALN GIQEEQ+VPARYRQEFLTI WEQVHLRSIFPFQYFS
Sbjct: 466 MLYLSPSRDEYYMVAAGNSLALNQGIQEEQVVPARYRQEFLTIAWEQVHLRSIFPFQYFS 525

Query: 61  IGASLIPFIEHNDANRALMSSNMQRQAVPLS 92
           IGASLIPFIEHNDANRALMSSNMQRQAVPLS
Sbjct: 526 IGASLIPFIEHNDANRALMSSNMQRQAVPLS 556

[Close]

BLAST of Lag0000584 vs. ExPASy Swiss-Prot
Match: Q2L8Z9 (DNA-directed RNA polymerase subunit beta OS=Gossypium hirsutum OX=3635 GN=rpoB PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.0e-43
Identity = 87/91 (95.60%), Postives = 88/91 (96.70%), Query Frame = 0


Query: 1   MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 60
           MLYLSPSRDEYYMVA GNSLALN GIQEEQ+VPARYRQEFLTI WEQVHLRSIFPFQYFS
Sbjct: 466 MLYLSPSRDEYYMVAAGNSLALNQGIQEEQVVPARYRQEFLTIAWEQVHLRSIFPFQYFS 525

Query: 61  IGASLIPFIEHNDANRALMSSNMQRQAVPLS 92
           IGASLIPFIEHNDANRALMSSNMQRQAVPLS
Sbjct: 526 IGASLIPFIEHNDANRALMSSNMQRQAVPLS 556

[Close]

BLAST of Lag0000584 vs. ExPASy Swiss-Prot
Match: Q0ZJ28 (DNA-directed RNA polymerase subunit beta OS=Vitis vinifera OX=29760 GN=rpoB PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.0e-43
Identity = 87/91 (95.60%), Postives = 88/91 (96.70%), Query Frame = 0


Query: 1   MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 60
           MLYLSPSRDEYYMVA GNSLALN GIQEEQ+VPARYRQEFLTI WEQVHLRSIFPFQYFS
Sbjct: 466 MLYLSPSRDEYYMVAAGNSLALNQGIQEEQVVPARYRQEFLTIAWEQVHLRSIFPFQYFS 525

Query: 61  IGASLIPFIEHNDANRALMSSNMQRQAVPLS 92
           IGASLIPFIEHNDANRALMSSNMQRQAVPLS
Sbjct: 526 IGASLIPFIEHNDANRALMSSNMQRQAVPLS 556

[Close]

BLAST of Lag0000584 vs. ExPASy Swiss-Prot
Match: A6MMB4 (DNA-directed RNA polymerase subunit beta OS=Chloranthus spicatus OX=13006 GN=rpoB PE=3 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 3.0e-43
Identity = 85/91 (93.41%), Postives = 88/91 (96.70%), Query Frame = 0


Query: 1   MLYLSPSRDEYYMVATGNSLALNPGIQEEQIVPARYRQEFLTIEWEQVHLRSIFPFQYFS 60
           M+YLSPSRDEYYMVA GNSLALN GIQEEQ+VPARYRQEFLTI WEQ+HLRSIFPFQYFS
Sbjct: 466 MVYLSPSRDEYYMVAAGNSLALNQGIQEEQVVPARYRQEFLTIAWEQIHLRSIFPFQYFS 525

Query: 61  IGASLIPFIEHNDANRALMSSNMQRQAVPLS 92
           IGASLIPFIEHNDANRALMSSNMQRQAVPLS
Sbjct: 526 IGASLIPFIEHNDANRALMSSNMQRQAVPLS 556

The following BLAST results are available for this feature:

BLAST of Lag0000584 vs. NCBI nr


Match Name	E-value	Identity	Description
YP_009752827.1	3.8e-43	100.00	RNA polymerase subunit beta [Baijiania yunnanensis] >QIT05899.1 RNA polymerase s...	[more]
AHM88854.1	3.8e-43	100.00	RNA polymerase beta subunit, partial [Lagenaria siceraria]	[more]
QJR52948.1	3.8e-43	100.00	RNA polymerase beta subunit [Herpetospermum pedunculosum]	[more]
YP_009753697.1	3.8e-43	100.00	RNA polymerase subunit beta [Trichosanthes nervifolia] >QIT05308.1 RNA polymeras...	[more]
YP_009752742.1	3.8e-43	100.00	RNA polymerase subunit beta [Cyclantheropsis parviflora] >QIT05392.1 RNA polymer...	[more]

BLAST of Lag0000584 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4VZP1	4.9e-46	100.00	DNA-directed RNA polymerase subunit beta OS=Cucumis sativus OX=3659 GN=rpoB PE=3...	[more]
A0ZZ27	1.0e-43	95.60	DNA-directed RNA polymerase subunit beta OS=Gossypium barbadense OX=3634 GN=rpoB...	[more]
Q2L8Z9	1.0e-43	95.60	DNA-directed RNA polymerase subunit beta OS=Gossypium hirsutum OX=3635 GN=rpoB P...	[more]
Q0ZJ28	1.0e-43	95.60	DNA-directed RNA polymerase subunit beta OS=Vitis vinifera OX=29760 GN=rpoB PE=3...	[more]
A6MMB4	3.0e-43	93.41	DNA-directed RNA polymerase subunit beta OS=Chloranthus spicatus OX=13006 GN=rpo...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037033	DNA-directed RNA polymerase, subunit 2, hybrid-binding domain superfamily	GENE3D	2.40.270.10		coord: 56..106 e-value: 1.1E-10 score: 43.3
None	No IPR available	PANTHER	PTHR20856:SF30	DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA	coord: 1..103
None	No IPR available	SUPERFAMILY	64484	beta and beta-prime subunits of DNA dependent RNA-polymerase	coord: 2..92
IPR015712	DNA-directed RNA polymerase, subunit 2	PANTHER	PTHR20856	DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2	coord: 1..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0000584.1	Lag0000584.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0032549	ribonucleoside binding