Lag0000288 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0000288
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionProtein kinase family protein
Locationchr4: 3016273 .. 3017262 (+)
RNA-Seq ExpressionLag0000288
SyntenyLag0000288
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATATAGAATCCATGATGAATTATGGACCAAAACGATACCATTACTCAGAGATAAAGAAGATCACCAACTCTTTTTCAGACAAAGTAGGTGAAGGTGGATATGGCATTGTCTATCGAGGTAAACTAGCTGATGGTACTCTCGTAGCTATAAAAGTTCTAAAGGTGTCAAAAGGTGATGGTGAAGATTTCATAAATGAAGTTACAAGTATTAGCAGAACATCGCATGTTAATATAGTTAGTCTTTTAGGATTTTCTTACAATACAAAGAAAATAACTCTAGTGTATGAGTTTATGGCTAATGGATCACTTGATAAGTTCATGTCTAGAAGTCACAATTTGGAGATGAAAATGCTTCATAGAATCATCATAGGTGTGGCAAGAGGCTTAGAGTATTTACACTGTGGGTGTAGTACAAGGATAGTGCATTTCGATATAAAACCACAAAACATTCTCTTGGATGAAGATTTCAACCCTAAAATATCTGACTTTGGCCTTGCAAAGCTTTGTAAAAGAAAAGAGAGTGCAATTTCAATGTTGGGAACTAGAGGAACAGCCGGGTTCATAGCGCCCGAAGTTTTTAGTCGTGCGTTTGGAGTTGTCTCGTACAAATCAGATGTGTATAGCTATGGGATGTTGGTTCTTGATCTCGTATTAGGAGGAATAAGAAATCCTAACTCGAGTTTGTTATCTGATGAAAGTGAACTGTACTTTCCAAATTGGGTTTTCAAGAAATTTGAAACGGATAAGACAACAAGAATGGATCAAAATTTGTTGGAAGAAGACGAAGAAATGGAGCGAAAAATGGTTATGATAGGTTTGTGGTGCATTCAAACGTCTCCATTAGATAGGCCTTCAATGAGTAGAGTATTGGAGATGTTGGAGGGAAGTATTCACTCCCTGCAAATGCCTCCGAGACCTTCATTGGTTCCCCATAATAGATCAATCCAACAATCTACCTCTGAATCAATATCACATGAGACTATTTAA

mRNA sequence

ATGAATATAGAATCCATGATGAATTATGGACCAAAACGATACCATTACTCAGAGATAAAGAAGATCACCAACTCTTTTTCAGACAAAGTAGGTGAAGGTGGATATGGCATTGTCTATCGAGGTAAACTAGCTGATGGTACTCTCGTAGCTATAAAAGTTCTAAAGGTGTCAAAAGGTGATGGTGAAGATTTCATAAATGAAGTTACAAGTATTAGCAGAACATCGCATGTTAATATAGTTAGTCTTTTAGGATTTTCTTACAATACAAAGAAAATAACTCTAGTGTATGAGTTTATGGCTAATGGATCACTTGATAAGTTCATGTCTAGAAGTCACAATTTGGAGATGAAAATGCTTCATAGAATCATCATAGGTGTGGCAAGAGGCTTAGAGTATTTACACTGTGGGTGTAGTACAAGGATAGTGCATTTCGATATAAAACCACAAAACATTCTCTTGGATGAAGATTTCAACCCTAAAATATCTGACTTTGGCCTTGCAAAGCTTTGTAAAAGAAAAGAGAGTGCAATTTCAATGTTGGGAACTAGAGGAACAGCCGGGTTCATAGCGCCCGAAGTTTTTAGTCGTGCGTTTGGAGTTGTCTCGTACAAATCAGATGTGTATAGCTATGGGATGTTGGTTCTTGATCTCGTATTAGGAGGAATAAGAAATCCTAACTCGAGTTTGTTATCTGATGAAAGTGAACTGTACTTTCCAAATTGGGTTTTCAAGAAATTTGAAACGGATAAGACAACAAGAATGGATCAAAATTTGTTGGAAGAAGACGAAGAAATGGAGCGAAAAATGGTTATGATAGGTTTGTGGTGCATTCAAACGTCTCCATTAGATAGGCCTTCAATGAGTAGAGTATTGGAGATGTTGGAGGGAAGTATTCACTCCCTGCAAATGCCTCCGAGACCTTCATTGGTTCCCCATAATAGATCAATCCAACAATCTACCTCTGAATCAATATCACATGAGACTATTTAA

Coding sequence (CDS)

ATGAATATAGAATCCATGATGAATTATGGACCAAAACGATACCATTACTCAGAGATAAAGAAGATCACCAACTCTTTTTCAGACAAAGTAGGTGAAGGTGGATATGGCATTGTCTATCGAGGTAAACTAGCTGATGGTACTCTCGTAGCTATAAAAGTTCTAAAGGTGTCAAAAGGTGATGGTGAAGATTTCATAAATGAAGTTACAAGTATTAGCAGAACATCGCATGTTAATATAGTTAGTCTTTTAGGATTTTCTTACAATACAAAGAAAATAACTCTAGTGTATGAGTTTATGGCTAATGGATCACTTGATAAGTTCATGTCTAGAAGTCACAATTTGGAGATGAAAATGCTTCATAGAATCATCATAGGTGTGGCAAGAGGCTTAGAGTATTTACACTGTGGGTGTAGTACAAGGATAGTGCATTTCGATATAAAACCACAAAACATTCTCTTGGATGAAGATTTCAACCCTAAAATATCTGACTTTGGCCTTGCAAAGCTTTGTAAAAGAAAAGAGAGTGCAATTTCAATGTTGGGAACTAGAGGAACAGCCGGGTTCATAGCGCCCGAAGTTTTTAGTCGTGCGTTTGGAGTTGTCTCGTACAAATCAGATGTGTATAGCTATGGGATGTTGGTTCTTGATCTCGTATTAGGAGGAATAAGAAATCCTAACTCGAGTTTGTTATCTGATGAAAGTGAACTGTACTTTCCAAATTGGGTTTTCAAGAAATTTGAAACGGATAAGACAACAAGAATGGATCAAAATTTGTTGGAAGAAGACGAAGAAATGGAGCGAAAAATGGTTATGATAGGTTTGTGGTGCATTCAAACGTCTCCATTAGATAGGCCTTCAATGAGTAGAGTATTGGAGATGTTGGAGGGAAGTATTCACTCCCTGCAAATGCCTCCGAGACCTTCATTGGTTCCCCATAATAGATCAATCCAACAATCTACCTCTGAATCAATATCACATGAGACTATTTAA

Protein sequence

MNIESMMNYGPKRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGEDFINEVTSISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMSRSHNLEMKMLHRIIIGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGFIAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSSLLSDESELYFPNWVFKKFETDKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPPRPSLVPHNRSIQQSTSESISHETI
Homology
BLAST of Lag0000288 vs. NCBI nr
Match: XP_011654383.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 [Cucumis sativus])

HSP 1 Score: 535.0 bits (1377), Expect = 4.5e-148
Identity = 269/328 (82.01%), Postives = 295/328 (89.94%), Query Frame = 0

Query: 3   IESMMNYGPKRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGE 62
           I+S++NYGPKRYHYSEIKK+TNSFS K+GEGGYGIVY+GKL DGTLVAIKVLK+SK +GE
Sbjct: 26  IDSIINYGPKRYHYSEIKKMTNSFSTKIGEGGYGIVYQGKLLDGTLVAIKVLKLSKANGE 85

Query: 63  DFINEVTSISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMSRSHNLEMKMLHRI 122
           DFINEV SISRTSHVNIV LLGF Y ++K  L+YEFMANGSLD+FMSRSHN EMKMLHRI
Sbjct: 86  DFINEVMSISRTSHVNIVGLLGFCYTSRKAALIYEFMANGSLDRFMSRSHNHEMKMLHRI 145

Query: 123 IIGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGT 182
           + GVARGLEYLHCGCSTRIVHFDIKPQNILLDED NPKISDFGLAKLCKRK SAISMLGT
Sbjct: 146 VTGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDSNPKISDFGLAKLCKRKVSAISMLGT 205

Query: 183 RGTAGFIAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIR-NPNSS--LLSDESELYFP 242
           RGTAGFIAPEVFS AFG+VSYKSDVYSYGMLVLDLVLGGIR NPN S  L  D+SE+YFP
Sbjct: 206 RGTAGFIAPEVFSPAFGIVSYKSDVYSYGMLVLDLVLGGIRNNPNRSRLLSDDDSEMYFP 265

Query: 243 NWVFKKFETDKTTRMDQNLL-EEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSI 302
           NWVFK  E  K+ RM Q+L+ EE+EEME+KM MIGLWCIQTSP+DRP+MSRVLEMLEGSI
Sbjct: 266 NWVFKNIEMSKSIRMRQSLMEEEEEEMEKKMTMIGLWCIQTSPIDRPTMSRVLEMLEGSI 325

Query: 303 HSLQMPPRPSLVPHNRSIQQSTSESISH 327
           HSLQMPPRP LV  N + QQSTSES+S+
Sbjct: 326 HSLQMPPRPLLVAPNMATQQSTSESLSY 353

BLAST of Lag0000288 vs. NCBI nr
Match: QKK82668.1 (LRK10L2 [Cucumis sativus])

HSP 1 Score: 534.6 bits (1376), Expect = 5.8e-148
Identity = 269/328 (82.01%), Postives = 295/328 (89.94%), Query Frame = 0

Query: 3   IESMMNYGPKRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGE 62
           I+S++NYGPKRYHYSEIKK+TNSFS K+GEGGYGIVY+GKL DGTLVAIKVLK+SK +GE
Sbjct: 306 IDSIINYGPKRYHYSEIKKMTNSFSTKIGEGGYGIVYQGKLLDGTLVAIKVLKLSKANGE 365

Query: 63  DFINEVTSISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMSRSHNLEMKMLHRI 122
           DFINEV SISRTSHVNIV LLGF Y ++K  L+YEFMANGSLD+FMSRSHN EMKMLHRI
Sbjct: 366 DFINEVMSISRTSHVNIVGLLGFCYTSRKAALIYEFMANGSLDRFMSRSHNHEMKMLHRI 425

Query: 123 IIGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGT 182
           + GVARGLEYLHCGCSTRIVHFDIKPQNILLDED NPKISDFGLAKLCKRK SAISMLGT
Sbjct: 426 VTGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDSNPKISDFGLAKLCKRKVSAISMLGT 485

Query: 183 RGTAGFIAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIR-NPNSS--LLSDESELYFP 242
           RGTAGFIAPEVFS AFG+VSYKSDVYSYGMLVLDLVLGGIR NPN S  L  D+SE+YFP
Sbjct: 486 RGTAGFIAPEVFSPAFGIVSYKSDVYSYGMLVLDLVLGGIRNNPNHSRLLSDDDSEMYFP 545

Query: 243 NWVFKKFETDKTTRMDQNLL-EEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSI 302
           NWVFK  E  K+ RM Q+L+ EE+EEME+KM MIGLWCIQTSP+DRP+MSRVLEMLEGSI
Sbjct: 546 NWVFKNIEMGKSIRMRQSLMEEEEEEMEKKMTMIGLWCIQTSPIDRPTMSRVLEMLEGSI 605

Query: 303 HSLQMPPRPSLVPHNRSIQQSTSESISH 327
           HSLQMPPRP LV  N + QQSTSES+S+
Sbjct: 606 HSLQMPPRPLLVAPNMATQQSTSESLSY 633

BLAST of Lag0000288 vs. NCBI nr
Match: KAE8649647.1 (hypothetical protein Csa_012280 [Cucumis sativus])

HSP 1 Score: 487.3 bits (1253), Expect = 1.1e-133
Identity = 247/301 (82.06%), Postives = 269/301 (89.37%), Query Frame = 0

Query: 30  VGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGEDFINEVTSISRTSHVNIVSLLGFSYNT 89
           +GEGGYGIVY+GKL DGTLVAIKVLK+SK +GEDFINEV SISRTSHVNIV LLGF Y +
Sbjct: 93  IGEGGYGIVYQGKLLDGTLVAIKVLKLSKANGEDFINEVMSISRTSHVNIVGLLGFCYTS 152

Query: 90  KKITLVYEFMANGSLDKFMSRSHNLEMKMLHRIIIGVARGLEYLHCGCSTRIVHFDIKPQ 149
           +K  L+YEFMANGSLD+FMSRSHN EMKMLHRI+ GVARGLEYLHCGCSTRIVHFDIKPQ
Sbjct: 153 RKAALIYEFMANGSLDRFMSRSHNHEMKMLHRIVTGVARGLEYLHCGCSTRIVHFDIKPQ 212

Query: 150 NILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGFIAPEVFSRAFGVVSYKSDVYS 209
           NILLDED NPKISDFGLAKLCKRK SAISMLGTRGTAGFIAPEVFS AFG+VSYKSDVYS
Sbjct: 213 NILLDEDSNPKISDFGLAKLCKRKVSAISMLGTRGTAGFIAPEVFSPAFGIVSYKSDVYS 272

Query: 210 YGMLVLDLVLGGIR-NPNSS--LLSDESELYFPNWVFKKFETDKTTRMDQNLL-EEDEEM 269
           YGMLVLDLVLGGIR NPN S  L  D+SE+YFPNWVFK  E  K+ RM Q+L+ EE+EEM
Sbjct: 273 YGMLVLDLVLGGIRNNPNRSRLLSDDDSEMYFPNWVFKNIEMSKSIRMRQSLMEEEEEEM 332

Query: 270 ERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPPRPSLVPHNRSIQQSTSESIS 327
           E+KM MIGLWCIQTSP+DRP+MSRVLEMLEGSIHSLQMPPRP LV  N + QQSTSES+S
Sbjct: 333 EKKMTMIGLWCIQTSPIDRPTMSRVLEMLEGSIHSLQMPPRPLLVAPNMATQQSTSESLS 392

BLAST of Lag0000288 vs. NCBI nr
Match: KAA8547784.1 (hypothetical protein F0562_004213 [Nyssa sinensis])

HSP 1 Score: 403.7 bits (1036), Expect = 1.6e-108
Identity = 197/317 (62.15%), Postives = 254/317 (80.13%), Query Frame = 0

Query: 11  PKRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGEDFINEVTS 70
           PK+Y YS++K+ITNSF DK+G+GGYG VY+GKL+DG LVA+K+L  +KG+G++FINEV +
Sbjct: 317 PKQYKYSDVKRITNSFIDKIGQGGYGSVYKGKLSDGLLVAVKLLSETKGNGDEFINEVAT 376

Query: 71  ISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFM-SRSHNLEMKMLHRIIIGVARG 130
           ISRTSH+N+V+LLGF Y   K  L+YEFM+NGSLDKF+ + S +LE K L++I IG+ARG
Sbjct: 377 ISRTSHINVVTLLGFCYERNKRALIYEFMSNGSLDKFIYNASVHLEWKTLYQIAIGIARG 436

Query: 131 LEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGFI 190
           LEYLH GC+TRIVHFDIKP NILLDE F PKISDFGLAKLCKR ES +SMLG RGT G+I
Sbjct: 437 LEYLHRGCNTRIVHFDIKPHNILLDEHFCPKISDFGLAKLCKRNESIVSMLGARGTVGYI 496

Query: 191 APEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSSLLSDESELYFPNWVFKKFETD 250
           APEVF R FG VS+KSDVYSYGM+VL++V  G+R  N   +   SE+YFP+W+F+  E  
Sbjct: 497 APEVFCRNFGRVSHKSDVYSYGMMVLEMV--GVRKKNEVEVVRSSEVYFPDWIFEHLEHG 556

Query: 251 KTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPPRPSL 310
              R+D  + EE+EE  RKM+++ LWCIQT+PL+RPSM++V+EMLEGS+ SLQ+PP+P L
Sbjct: 557 ADARLDGVITEEEEETARKMIVVSLWCIQTNPLERPSMNKVVEMLEGSLQSLQIPPKPFL 616

Query: 311 VPHNRSIQQSTSESISH 327
               R + Q +S S+S+
Sbjct: 617 FSPKR-LAQDSSASLSY 630

BLAST of Lag0000288 vs. NCBI nr
Match: KAF3955879.1 (hypothetical protein CMV_018949 [Castanea mollissima])

HSP 1 Score: 401.0 bits (1029), Expect = 1.0e-107
Identity = 196/328 (59.76%), Postives = 257/328 (78.35%), Query Frame = 0

Query: 6   MMNYG---PKRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGE 65
           +MN G   PK+Y YS+IKK+T+SF +K+G+GGYG VY+GKL D  LVA+KV+   KGDGE
Sbjct: 528 IMNQGSLAPKQYRYSDIKKMTHSFKNKLGQGGYGSVYKGKLLDNHLVAVKVINDLKGDGE 587

Query: 66  DFINEVTSISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFM------SRSHNLEM 125
           DFINE+ SISRTSHVN+V+LLG+ Y   K  LVYEFM NGSLDKF+      S +  LE 
Sbjct: 588 DFINEIASISRTSHVNVVTLLGYCYERTKRALVYEFMPNGSLDKFICNPESSSTNCKLEH 647

Query: 126 KMLHRIIIGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESA 185
           K L  I +GVARGLEYLH GCSTRI+HFDIKPQNILLD+DF PKISDFGLA+LC++KES 
Sbjct: 648 KTLFEIAVGVARGLEYLHRGCSTRILHFDIKPQNILLDDDFCPKISDFGLARLCQKKESI 707

Query: 186 ISMLGTRGTAGFIAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSSLLSDESEL 245
           +SMLGTRGT G+IAPEVFSR FG VS+KSDVYSYGML+L++V  G++    + +S  +E 
Sbjct: 708 VSMLGTRGTIGYIAPEVFSRNFGGVSHKSDVYSYGMLILEMV--GVKKNFDAEVSHTTER 767

Query: 246 YFPNWVFKKFETDKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEG 305
           YFPNW+++     +    +  ++EE+E+M+RKM+++GLWCIQT P +RPSM++V+EMLEG
Sbjct: 768 YFPNWIYQNLNLGENVENNMVIIEEEEQMKRKMILVGLWCIQTIPTNRPSMTKVVEMLEG 827

Query: 306 SIHSLQMPPRPSLVPHNRSIQQSTSESI 325
           ++HS+++PP+P L    RS+Q S++ S+
Sbjct: 828 NVHSIEIPPKPFLFSPARSVQYSSTTSL 853

BLAST of Lag0000288 vs. ExPASy Swiss-Prot
Match: F4HQ22 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.4 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 2.9e-102
Identity = 192/325 (59.08%), Postives = 248/325 (76.31%), Query Frame = 0

Query: 12  KRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKG-DGEDFINEVTS 71
           K+Y Y ++K+ITNSF++ VG GG+GIVYRG L+DG +VA+KVLK  KG +GEDFINEV S
Sbjct: 295 KQYSYEQVKRITNSFAEVVGRGGFGIVYRGTLSDGRMVAVKVLKDLKGNNGEDFINEVAS 354

Query: 72  ISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMS--RSHNLEMKMLHRIIIGVAR 131
           +S+TSHVNIV+LLGF     K  ++YEFM NGSLDKF+S  +S  ++ + L+ I +GVAR
Sbjct: 355 MSQTSHVNIVTLLGFCSEGYKRAIIYEFMENGSLDKFISSKKSSTMDWRELYGIALGVAR 414

Query: 132 GLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGF 191
           GLEYLH GC TRIVHFDIKPQN+LLD++ +PK+SDFGLAKLC+RKES +S++ TRGT G+
Sbjct: 415 GLEYLHHGCRTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCERKESILSLMDTRGTIGY 474

Query: 192 IAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSS--LLSDESELYFPNWVFKKF 251
           IAPEVFSR +G VS+KSDVYSYGMLVLD++  G RN  S+    S  S +YFP W++K  
Sbjct: 475 IAPEVFSRVYGSVSHKSDVYSYGMLVLDII--GARNKTSTEDTTSSTSSMYFPEWIYKDL 534

Query: 252 ETDKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPPR 311
           E     R+  N  EED E+ +KM ++GLWCIQ  PLDRP+M+RV+EM+EG++ +L++PPR
Sbjct: 535 EKGDNGRLIVNRSEED-EIAKKMTLVGLWCIQPWPLDRPAMNRVVEMMEGNLDALEVPPR 594

Query: 312 P----SLVPHNRSIQQSTSESISHE 328
           P    S+VPH  S   S   SIS E
Sbjct: 595 PVLQCSVVPHLDSSWISEENSISSE 616

BLAST of Lag0000288 vs. ExPASy Swiss-Prot
Match: D7SFH9 (Protein SUPPRESSOR OF NPR1-1 CONSTITUTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.6 PE=1 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.0e-99
Identity = 176/304 (57.89%), Postives = 239/304 (78.62%), Query Frame = 0

Query: 12   KRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGEDFINEVTSI 71
            + Y Y+++K+IT SF++ VG GG+GIVY+G L+DG +VA+KVLK +KG+GEDFINEV ++
Sbjct: 793  EHYTYAQVKRITKSFAEVVGRGGFGIVYKGTLSDGRVVAVKVLKDTKGNGEDFINEVATM 852

Query: 72   SRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFM--SRSHNLEMKMLHRIIIGVARG 131
            SRTSH+NIVSLLGF     K  ++YEF+ NGSLDKF+    S N++   L+RI +GVA G
Sbjct: 853  SRTSHLNIVSLLGFCSEGSKRAIIYEFLENGSLDKFILGKTSVNMDWTALYRIALGVAHG 912

Query: 132  LEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGFI 191
            LEYLH  C TRIVHFDIKPQN+LLD+ F PK+SDFGLAKLC++KES +SML TRGT G+I
Sbjct: 913  LEYLHHSCKTRIVHFDIKPQNVLLDDSFCPKVSDFGLAKLCEKKESILSMLDTRGTIGYI 972

Query: 192  APEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRN---PNSSLLSDESELYFPNWVFKKF 251
            APE+ SR +G VS+KSDVYSYGMLVL+++  G RN    N +  S+ S +YFP WV++  
Sbjct: 973  APEMISRVYGNVSHKSDVYSYGMLVLEII--GARNKEKANQACASNTSSMYFPEWVYRDL 1032

Query: 252  ETDKTTR-MDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPP 310
            E+ K+ R ++  +  E++E+ +KM ++GLWCIQ SP+DRP+M+RV+EM+EGS+ +L++PP
Sbjct: 1033 ESCKSGRHIEDGINSEEDELAKKMTLVGLWCIQPSPVDRPAMNRVVEMMEGSLEALEVPP 1092

BLAST of Lag0000288 vs. ExPASy Swiss-Prot
Match: Q3ECH2 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.8 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.8 PE=2 SV=2)

HSP 1 Score: 360.1 bits (923), Expect = 2.6e-98
Identity = 184/329 (55.93%), Postives = 245/329 (74.47%), Query Frame = 0

Query: 12  KRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKG-DGEDFINEVTS 71
           K Y Y+E+KK+T SF++ VG GG+GIVY G L+D ++VA+KVLK SKG DGEDFINEV S
Sbjct: 544 KHYTYAEVKKMTKSFTEVVGRGGFGIVYSGTLSDSSMVAVKVLKDSKGTDGEDFINEVAS 603

Query: 72  ISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMS--RSHNLEMKMLHRIIIGVAR 131
           +S+TSHVNIVSLLGF     +  ++YEF+ NGSLDKF+S   S NL++K L+ I +GVAR
Sbjct: 604 MSQTSHVNIVSLLGFCCEGSRRAIIYEFLGNGSLDKFISDKSSVNLDLKTLYGIALGVAR 663

Query: 132 GLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGF 191
           GLEYLH GC TRIVHFDIKPQN+LLD++  PK+SDFGLAKLC++KES +S+L TRGT G+
Sbjct: 664 GLEYLHYGCKTRIVHFDIKPQNVLLDDNLCPKVSDFGLAKLCEKKESILSLLDTRGTIGY 723

Query: 192 IAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNP---NSSLLSDESELYFPNWVFKK 251
           IAPE+ SR +G VS+KSDVYSYGMLVL+++  G R     + +  SD S +YFP W++K 
Sbjct: 724 IAPEMISRLYGSVSHKSDVYSYGMLVLEMI--GARKKERFDQNSRSDGSSIYFPEWIYKD 783

Query: 252 FE---------TDKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEG 311
            E         T+    ++  +  E+EE+ RKM ++GLWCIQ+SP DRP M++V+EM+EG
Sbjct: 784 LEKANIKDIEKTENGGLIENGISSEEEEIARKMTLVGLWCIQSSPSDRPPMNKVVEMMEG 843

Query: 312 SIHSLQMPPRPSLVPHNRSIQQSTSESIS 326
           S+ +L++PPRP L       QQ ++ S+S
Sbjct: 844 SLDALEVPPRPVL-------QQISASSVS 863

BLAST of Lag0000288 vs. ExPASy Swiss-Prot
Match: Q9FF31 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.1 PE=3 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 3.4e-98
Identity = 172/301 (57.14%), Postives = 231/301 (76.74%), Query Frame = 0

Query: 12  KRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGEDFINEVTSI 71
           K+Y Y+E++KIT  FS  +G+GG+G VY G L DG  VA+K+LK  K +GEDFINEV S+
Sbjct: 309 KQYSYAEVRKITKLFSHTLGKGGFGTVYGGNLCDGRKVAVKILKDFKSNGEDFINEVASM 368

Query: 72  SRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMS--RSHNLEMKMLHRIIIGVARG 131
           S+TSHVNIVSLLGF Y   K  +VYEF+ NGSLD+F+S  +S NL++  L+RI +GVARG
Sbjct: 369 SQTSHVNIVSLLGFCYEGSKRAIVYEFLENGSLDQFLSEKKSLNLDVSTLYRIALGVARG 428

Query: 132 LEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGFI 191
           L+YLH GC TRIVHFDIKPQNILLD+ F PK+SDFGLAKLC+++ES +S+L  RGT G+I
Sbjct: 429 LDYLHHGCKTRIVHFDIKPQNILLDDTFCPKVSDFGLAKLCEKRESILSLLDARGTIGYI 488

Query: 192 APEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSSLLSDESELYFPNWVFKKFET- 251
           APEVFS  +G VS+KSDVYSYGMLVL+++    +    +  S+ S  YFP+W++K  E  
Sbjct: 489 APEVFSGMYGRVSHKSDVYSYGMLVLEMIGAKNKEIEETAASNSSSAYFPDWIYKNLENG 548

Query: 252 DKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPPRPS 310
           + T +    +  ED+E+ +KM ++GLWCIQ SPL+RP M+R++EM+EGS+  L++PP+PS
Sbjct: 549 EDTWKFGDEISREDKEVAKKMTLVGLWCIQPSPLNRPPMNRIVEMMEGSLDVLEVPPKPS 608

BLAST of Lag0000288 vs. ExPASy Swiss-Prot
Match: F4HQ20 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.5 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.5 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.3e-97
Identity = 182/325 (56.00%), Postives = 245/325 (75.38%), Query Frame = 0

Query: 12  KRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGEDFINEVTSI 71
           K Y Y+++  IT SF++ +G+GG+G VYRG L DG  VA+KVLK S+G+GEDFINEV S+
Sbjct: 336 KHYSYAQVTSITKSFAEVIGKGGFGTVYRGTLYDGRSVAVKVLKESQGNGEDFINEVASM 395

Query: 72  SRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMS--RSHNLEMKMLHRIIIGVARG 131
           S+TSHVNIV+LLGF     K  ++YEFM NGSLDKF+S  +S  ++ + L+ I +GVARG
Sbjct: 396 SQTSHVNIVTLLGFCSEGYKRAIIYEFMENGSLDKFISSKKSSTMDWRELYGIALGVARG 455

Query: 132 LEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGFI 191
           LEYLH GC TRIVHFDIKPQN+LLD++ +PK+SDFGLAKLC+RKES +S++ TRGT G+I
Sbjct: 456 LEYLHHGCRTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCERKESILSLMDTRGTIGYI 515

Query: 192 APEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSS--LLSDESELYFPNWVFKKFE 251
           APEVFSR +G VS+KSDVYSYGMLVLD++  G RN  S+    S  S +YFP W+++  E
Sbjct: 516 APEVFSRVYGRVSHKSDVYSYGMLVLDII--GARNKTSTEDTTSSTSSMYFPEWIYRDLE 575

Query: 252 TDKTTR-MDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPPR 311
                + ++  +  E++E+ +KM ++GLWCIQ  PLDRP+M+RV+EM+EG++ +L++PPR
Sbjct: 576 KAHNGKSIETAISNEEDEIAKKMTLVGLWCIQPWPLDRPAMNRVVEMMEGNLDALEVPPR 635

Query: 312 PSL--VPHNRSIQQST-SESISHET 329
           P L  +P     + ST SE IS  T
Sbjct: 636 PVLQQIPTATLQESSTFSEDISAYT 658

BLAST of Lag0000288 vs. ExPASy TrEMBL
Match: A0A6M9BN16 (LRK10L2 OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 2.8e-148
Identity = 269/328 (82.01%), Postives = 295/328 (89.94%), Query Frame = 0

Query: 3   IESMMNYGPKRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGE 62
           I+S++NYGPKRYHYSEIKK+TNSFS K+GEGGYGIVY+GKL DGTLVAIKVLK+SK +GE
Sbjct: 306 IDSIINYGPKRYHYSEIKKMTNSFSTKIGEGGYGIVYQGKLLDGTLVAIKVLKLSKANGE 365

Query: 63  DFINEVTSISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMSRSHNLEMKMLHRI 122
           DFINEV SISRTSHVNIV LLGF Y ++K  L+YEFMANGSLD+FMSRSHN EMKMLHRI
Sbjct: 366 DFINEVMSISRTSHVNIVGLLGFCYTSRKAALIYEFMANGSLDRFMSRSHNHEMKMLHRI 425

Query: 123 IIGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGT 182
           + GVARGLEYLHCGCSTRIVHFDIKPQNILLDED NPKISDFGLAKLCKRK SAISMLGT
Sbjct: 426 VTGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDSNPKISDFGLAKLCKRKVSAISMLGT 485

Query: 183 RGTAGFIAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIR-NPNSS--LLSDESELYFP 242
           RGTAGFIAPEVFS AFG+VSYKSDVYSYGMLVLDLVLGGIR NPN S  L  D+SE+YFP
Sbjct: 486 RGTAGFIAPEVFSPAFGIVSYKSDVYSYGMLVLDLVLGGIRNNPNHSRLLSDDDSEMYFP 545

Query: 243 NWVFKKFETDKTTRMDQNLL-EEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSI 302
           NWVFK  E  K+ RM Q+L+ EE+EEME+KM MIGLWCIQTSP+DRP+MSRVLEMLEGSI
Sbjct: 546 NWVFKNIEMGKSIRMRQSLMEEEEEEMEKKMTMIGLWCIQTSPIDRPTMSRVLEMLEGSI 605

Query: 303 HSLQMPPRPSLVPHNRSIQQSTSESISH 327
           HSLQMPPRP LV  N + QQSTSES+S+
Sbjct: 606 HSLQMPPRPLLVAPNMATQQSTSESLSY 633

BLAST of Lag0000288 vs. ExPASy TrEMBL
Match: A0A2N9I4J7 (Protein kinase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS48869 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 4.3e-112
Identity = 207/329 (62.92%), Postives = 261/329 (79.33%), Query Frame = 0

Query: 6    MMNYG---PKRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGE 65
            +MN+G   PKRY YSEIKK+TNSF +K+G+GGYG VY+GKL DG LVA+KV+   KG GE
Sbjct: 1455 IMNHGSFAPKRYTYSEIKKMTNSFKEKLGQGGYGSVYKGKLLDGRLVAVKVINELKGSGE 1514

Query: 66   DFINEVTSISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFM------SRSHNLEM 125
            +FINEV SISRTSHVNIV+LLG+SY T K  LVYEFM NGSLDKF+      S +  LE 
Sbjct: 1515 EFINEVASISRTSHVNIVTLLGYSYETTKRALVYEFMPNGSLDKFIYNHEPSSTNCQLER 1574

Query: 126  KMLHRIIIGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESA 185
            + L +I++G+ARGLEYLH GCSTRI+HFDIKPQNILLDEDF PKISDFGLA+LC++KES 
Sbjct: 1575 EKLFQIVVGIARGLEYLHRGCSTRILHFDIKPQNILLDEDFCPKISDFGLARLCQKKESI 1634

Query: 186  ISMLGTRGTAGFIAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSSLLSDESEL 245
            ISMLGTRGT G+IAPEVFSR FG VSYK+DVYSYGMLVL++V  G R  ++ ++S  SE 
Sbjct: 1635 ISMLGTRGTIGYIAPEVFSRNFGGVSYKADVYSYGMLVLEMV--GRRKNSNVVMSHSSEN 1694

Query: 246  YFPNWVFKKFETDKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEG 305
            YFP+W++++   +       +++EE EEMERKM+++ LWCIQT P DRPSM++V+EMLEG
Sbjct: 1695 YFPHWIYQELNLNVNVGNHMDMMEEGEEMERKMILVSLWCIQTVPADRPSMTKVVEMLEG 1754

Query: 306  SIHSLQMPPRPSLVPHNRSIQQSTSESIS 326
             + S+++PP+P L    RSIQ S++ S+S
Sbjct: 1755 GLQSIEIPPKPFLSSPKRSIQNSSTTSLS 1781

BLAST of Lag0000288 vs. ExPASy TrEMBL
Match: A0A2N9FST5 (Protein kinase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS18087 PE=3 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 9.5e-112
Identity = 206/329 (62.61%), Postives = 261/329 (79.33%), Query Frame = 0

Query: 6   MMNYG---PKRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGE 65
           +MN+G   PKRY YSEIKK+TNSF +K+G+GGYG VY+GKL DG LVA+KV+   KG GE
Sbjct: 19  IMNHGSFAPKRYTYSEIKKMTNSFKEKLGQGGYGSVYKGKLLDGRLVAVKVINELKGSGE 78

Query: 66  DFINEVTSISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFM------SRSHNLEM 125
           +FINEV SISRTSHVNIV+LLG+SY T K  LVYEFM NGSLDKF+      S +  LE 
Sbjct: 79  EFINEVASISRTSHVNIVTLLGYSYETTKRALVYEFMPNGSLDKFIYNHEPSSTNCQLER 138

Query: 126 KMLHRIIIGVARGLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESA 185
           + L +I++G+ARGLEYLH GCSTRI+HFDIKPQNILLDEDF PKISDFGLA+LC++KES 
Sbjct: 139 EKLFQIVVGIARGLEYLHRGCSTRILHFDIKPQNILLDEDFCPKISDFGLARLCQKKESI 198

Query: 186 ISMLGTRGTAGFIAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSSLLSDESEL 245
           +SMLGTRGT G+IAPEVFSR FG VSYK+DVYSYGMLVL++V  G R  ++ ++S  SE 
Sbjct: 199 VSMLGTRGTIGYIAPEVFSRNFGGVSYKADVYSYGMLVLEMV--GRRKNSNVVMSHSSEN 258

Query: 246 YFPNWVFKKFETDKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEG 305
           YFP+W++++   +       +++EE EEMERKM+++ LWCIQT P DRPSM++V+EMLEG
Sbjct: 259 YFPHWIYQELNLNVNVGNHMDMMEEGEEMERKMILVSLWCIQTVPPDRPSMTKVVEMLEG 318

Query: 306 SIHSLQMPPRPSLVPHNRSIQQSTSESIS 326
            + S+++PP+P L    RSIQ S++ S+S
Sbjct: 319 GLQSIEIPPKPFLSSPKRSIQNSSTTSLS 345

BLAST of Lag0000288 vs. ExPASy TrEMBL
Match: A0A5B7C138 (Protein kinase domain-containing protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_043372 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 4.0e-110
Identity = 197/305 (64.59%), Postives = 248/305 (81.31%), Query Frame = 0

Query: 11  PKRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGEDFINEVTS 70
           PK+Y+YS++K+ITNSF DK+G+GGYG VY+GKL+DG LVA+K+L  +KG+G++FINEV S
Sbjct: 30  PKQYYYSDVKRITNSFVDKLGQGGYGSVYKGKLSDGHLVAVKLLSETKGNGDEFINEVAS 89

Query: 71  ISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMSR------SHNLEMKMLHRIII 130
           ISRTSH+N+V+LLGF Y   K  L+YEFM NGSLDKF+S       +++LE K L++I I
Sbjct: 90  ISRTSHINVVTLLGFCYERNKRALIYEFMPNGSLDKFISNDGSLNTNYHLEWKTLYQIAI 149

Query: 131 GVARGLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRG 190
           G+ARGLEYLH GC+TRIVHFDIKPQNILLDEDF PKISDFGLAKLCK+KES +SMLG RG
Sbjct: 150 GIARGLEYLHRGCNTRIVHFDIKPQNILLDEDFCPKISDFGLAKLCKKKESIVSMLGARG 209

Query: 191 TAGFIAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSSLLSDESELYFPNWVFK 250
           TAG+IAPEVFSR+FG VS+KSDVYSYGM+VL++V  G+R  N   + D SE YFP+W+++
Sbjct: 210 TAGYIAPEVFSRSFGGVSHKSDVYSYGMMVLEMV--GVRKNNEVEVVDSSERYFPDWIYE 269

Query: 251 KFETDKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMP 310
             E D   R+D    EE++E  RKM ++ LWCIQT+P DRPSMSRV+EMLEG +  LQ+P
Sbjct: 270 YLEHDTDIRLDSVTTEEEKETARKMTLVSLWCIQTNPSDRPSMSRVVEMLEGGLLCLQIP 329

BLAST of Lag0000288 vs. ExPASy TrEMBL
Match: A0A5J5BYQ6 (Protein kinase domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_004213 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 7.5e-109
Identity = 197/317 (62.15%), Postives = 254/317 (80.13%), Query Frame = 0

Query: 11  PKRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGEDFINEVTS 70
           PK+Y YS++K+ITNSF DK+G+GGYG VY+GKL+DG LVA+K+L  +KG+G++FINEV +
Sbjct: 317 PKQYKYSDVKRITNSFIDKIGQGGYGSVYKGKLSDGLLVAVKLLSETKGNGDEFINEVAT 376

Query: 71  ISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFM-SRSHNLEMKMLHRIIIGVARG 130
           ISRTSH+N+V+LLGF Y   K  L+YEFM+NGSLDKF+ + S +LE K L++I IG+ARG
Sbjct: 377 ISRTSHINVVTLLGFCYERNKRALIYEFMSNGSLDKFIYNASVHLEWKTLYQIAIGIARG 436

Query: 131 LEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGFI 190
           LEYLH GC+TRIVHFDIKP NILLDE F PKISDFGLAKLCKR ES +SMLG RGT G+I
Sbjct: 437 LEYLHRGCNTRIVHFDIKPHNILLDEHFCPKISDFGLAKLCKRNESIVSMLGARGTVGYI 496

Query: 191 APEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSSLLSDESELYFPNWVFKKFETD 250
           APEVF R FG VS+KSDVYSYGM+VL++V  G+R  N   +   SE+YFP+W+F+  E  
Sbjct: 497 APEVFCRNFGRVSHKSDVYSYGMMVLEMV--GVRKKNEVEVVRSSEVYFPDWIFEHLEHG 556

Query: 251 KTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPPRPSL 310
              R+D  + EE+EE  RKM+++ LWCIQT+PL+RPSM++V+EMLEGS+ SLQ+PP+P L
Sbjct: 557 ADARLDGVITEEEEETARKMIVVSLWCIQTNPLERPSMNKVVEMLEGSLQSLQIPPKPFL 616

Query: 311 VPHNRSIQQSTSESISH 327
               R + Q +S S+S+
Sbjct: 617 FSPKR-LAQDSSASLSY 630

BLAST of Lag0000288 vs. TAIR 10
Match: AT1G66920.1 (Protein kinase superfamily protein )

HSP 1 Score: 373.2 bits (957), Expect = 2.1e-103
Identity = 192/325 (59.08%), Postives = 248/325 (76.31%), Query Frame = 0

Query: 12  KRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKG-DGEDFINEVTS 71
           K+Y Y ++K+ITNSF++ VG GG+GIVYRG L+DG +VA+KVLK  KG +GEDFINEV S
Sbjct: 287 KQYSYEQVKRITNSFAEVVGRGGFGIVYRGTLSDGRMVAVKVLKDLKGNNGEDFINEVAS 346

Query: 72  ISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMS--RSHNLEMKMLHRIIIGVAR 131
           +S+TSHVNIV+LLGF     K  ++YEFM NGSLDKF+S  +S  ++ + L+ I +GVAR
Sbjct: 347 MSQTSHVNIVTLLGFCSEGYKRAIIYEFMENGSLDKFISSKKSSTMDWRELYGIALGVAR 406

Query: 132 GLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGF 191
           GLEYLH GC TRIVHFDIKPQN+LLD++ +PK+SDFGLAKLC+RKES +S++ TRGT G+
Sbjct: 407 GLEYLHHGCRTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCERKESILSLMDTRGTIGY 466

Query: 192 IAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSS--LLSDESELYFPNWVFKKF 251
           IAPEVFSR +G VS+KSDVYSYGMLVLD++  G RN  S+    S  S +YFP W++K  
Sbjct: 467 IAPEVFSRVYGSVSHKSDVYSYGMLVLDII--GARNKTSTEDTTSSTSSMYFPEWIYKDL 526

Query: 252 ETDKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPPR 311
           E     R+  N  EED E+ +KM ++GLWCIQ  PLDRP+M+RV+EM+EG++ +L++PPR
Sbjct: 527 EKGDNGRLIVNRSEED-EIAKKMTLVGLWCIQPWPLDRPAMNRVVEMMEGNLDALEVPPR 586

Query: 312 P----SLVPHNRSIQQSTSESISHE 328
           P    S+VPH  S   S   SIS E
Sbjct: 587 PVLQCSVVPHLDSSWISEENSISSE 608

BLAST of Lag0000288 vs. TAIR 10
Match: AT1G66920.2 (Protein kinase superfamily protein )

HSP 1 Score: 373.2 bits (957), Expect = 2.1e-103
Identity = 192/325 (59.08%), Postives = 248/325 (76.31%), Query Frame = 0

Query: 12  KRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKG-DGEDFINEVTS 71
           K+Y Y ++K+ITNSF++ VG GG+GIVYRG L+DG +VA+KVLK  KG +GEDFINEV S
Sbjct: 295 KQYSYEQVKRITNSFAEVVGRGGFGIVYRGTLSDGRMVAVKVLKDLKGNNGEDFINEVAS 354

Query: 72  ISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMS--RSHNLEMKMLHRIIIGVAR 131
           +S+TSHVNIV+LLGF     K  ++YEFM NGSLDKF+S  +S  ++ + L+ I +GVAR
Sbjct: 355 MSQTSHVNIVTLLGFCSEGYKRAIIYEFMENGSLDKFISSKKSSTMDWRELYGIALGVAR 414

Query: 132 GLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGF 191
           GLEYLH GC TRIVHFDIKPQN+LLD++ +PK+SDFGLAKLC+RKES +S++ TRGT G+
Sbjct: 415 GLEYLHHGCRTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCERKESILSLMDTRGTIGY 474

Query: 192 IAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSS--LLSDESELYFPNWVFKKF 251
           IAPEVFSR +G VS+KSDVYSYGMLVLD++  G RN  S+    S  S +YFP W++K  
Sbjct: 475 IAPEVFSRVYGSVSHKSDVYSYGMLVLDII--GARNKTSTEDTTSSTSSMYFPEWIYKDL 534

Query: 252 ETDKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPPR 311
           E     R+  N  EED E+ +KM ++GLWCIQ  PLDRP+M+RV+EM+EG++ +L++PPR
Sbjct: 535 EKGDNGRLIVNRSEED-EIAKKMTLVGLWCIQPWPLDRPAMNRVVEMMEGNLDALEVPPR 594

Query: 312 P----SLVPHNRSIQQSTSESISHE 328
           P    S+VPH  S   S   SIS E
Sbjct: 595 PVLQCSVVPHLDSSWISEENSISSE 616

BLAST of Lag0000288 vs. TAIR 10
Match: AT1G66980.1 (suppressor of npr1-1 constitutive 4 )

HSP 1 Score: 364.8 bits (935), Expect = 7.4e-101
Identity = 176/304 (57.89%), Postives = 239/304 (78.62%), Query Frame = 0

Query: 12   KRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGEDFINEVTSI 71
            + Y Y+++K+IT SF++ VG GG+GIVY+G L+DG +VA+KVLK +KG+GEDFINEV ++
Sbjct: 793  EHYTYAQVKRITKSFAEVVGRGGFGIVYKGTLSDGRVVAVKVLKDTKGNGEDFINEVATM 852

Query: 72   SRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFM--SRSHNLEMKMLHRIIIGVARG 131
            SRTSH+NIVSLLGF     K  ++YEF+ NGSLDKF+    S N++   L+RI +GVA G
Sbjct: 853  SRTSHLNIVSLLGFCSEGSKRAIIYEFLENGSLDKFILGKTSVNMDWTALYRIALGVAHG 912

Query: 132  LEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGFI 191
            LEYLH  C TRIVHFDIKPQN+LLD+ F PK+SDFGLAKLC++KES +SML TRGT G+I
Sbjct: 913  LEYLHHSCKTRIVHFDIKPQNVLLDDSFCPKVSDFGLAKLCEKKESILSMLDTRGTIGYI 972

Query: 192  APEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRN---PNSSLLSDESELYFPNWVFKKF 251
            APE+ SR +G VS+KSDVYSYGMLVL+++  G RN    N +  S+ S +YFP WV++  
Sbjct: 973  APEMISRVYGNVSHKSDVYSYGMLVLEII--GARNKEKANQACASNTSSMYFPEWVYRDL 1032

Query: 252  ETDKTTR-MDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPP 310
            E+ K+ R ++  +  E++E+ +KM ++GLWCIQ SP+DRP+M+RV+EM+EGS+ +L++PP
Sbjct: 1033 ESCKSGRHIEDGINSEEDELAKKMTLVGLWCIQPSPVDRPAMNRVVEMMEGSLEALEVPP 1092

BLAST of Lag0000288 vs. TAIR 10
Match: AT1G67000.1 (Protein kinase superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.8e-99
Identity = 184/329 (55.93%), Postives = 245/329 (74.47%), Query Frame = 0

Query: 12  KRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKG-DGEDFINEVTS 71
           K Y Y+E+KK+T SF++ VG GG+GIVY G L+D ++VA+KVLK SKG DGEDFINEV S
Sbjct: 544 KHYTYAEVKKMTKSFTEVVGRGGFGIVYSGTLSDSSMVAVKVLKDSKGTDGEDFINEVAS 603

Query: 72  ISRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMS--RSHNLEMKMLHRIIIGVAR 131
           +S+TSHVNIVSLLGF     +  ++YEF+ NGSLDKF+S   S NL++K L+ I +GVAR
Sbjct: 604 MSQTSHVNIVSLLGFCCEGSRRAIIYEFLGNGSLDKFISDKSSVNLDLKTLYGIALGVAR 663

Query: 132 GLEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGF 191
           GLEYLH GC TRIVHFDIKPQN+LLD++  PK+SDFGLAKLC++KES +S+L TRGT G+
Sbjct: 664 GLEYLHYGCKTRIVHFDIKPQNVLLDDNLCPKVSDFGLAKLCEKKESILSLLDTRGTIGY 723

Query: 192 IAPEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNP---NSSLLSDESELYFPNWVFKK 251
           IAPE+ SR +G VS+KSDVYSYGMLVL+++  G R     + +  SD S +YFP W++K 
Sbjct: 724 IAPEMISRLYGSVSHKSDVYSYGMLVLEMI--GARKKERFDQNSRSDGSSIYFPEWIYKD 783

Query: 252 FE---------TDKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEG 311
            E         T+    ++  +  E+EE+ RKM ++GLWCIQ+SP DRP M++V+EM+EG
Sbjct: 784 LEKANIKDIEKTENGGLIENGISSEEEEIARKMTLVGLWCIQSSPSDRPPMNKVVEMMEG 843

Query: 312 SIHSLQMPPRPSLVPHNRSIQQSTSESIS 326
           S+ +L++PPRP L       QQ ++ S+S
Sbjct: 844 SLDALEVPPRPVL-------QQISASSVS 863

BLAST of Lag0000288 vs. TAIR 10
Match: AT5G38260.1 (Protein kinase superfamily protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.4e-99
Identity = 172/301 (57.14%), Postives = 231/301 (76.74%), Query Frame = 0

Query: 12  KRYHYSEIKKITNSFSDKVGEGGYGIVYRGKLADGTLVAIKVLKVSKGDGEDFINEVTSI 71
           K+Y Y+E++KIT  FS  +G+GG+G VY G L DG  VA+K+LK  K +GEDFINEV S+
Sbjct: 309 KQYSYAEVRKITKLFSHTLGKGGFGTVYGGNLCDGRKVAVKILKDFKSNGEDFINEVASM 368

Query: 72  SRTSHVNIVSLLGFSYNTKKITLVYEFMANGSLDKFMS--RSHNLEMKMLHRIIIGVARG 131
           S+TSHVNIVSLLGF Y   K  +VYEF+ NGSLD+F+S  +S NL++  L+RI +GVARG
Sbjct: 369 SQTSHVNIVSLLGFCYEGSKRAIVYEFLENGSLDQFLSEKKSLNLDVSTLYRIALGVARG 428

Query: 132 LEYLHCGCSTRIVHFDIKPQNILLDEDFNPKISDFGLAKLCKRKESAISMLGTRGTAGFI 191
           L+YLH GC TRIVHFDIKPQNILLD+ F PK+SDFGLAKLC+++ES +S+L  RGT G+I
Sbjct: 429 LDYLHHGCKTRIVHFDIKPQNILLDDTFCPKVSDFGLAKLCEKRESILSLLDARGTIGYI 488

Query: 192 APEVFSRAFGVVSYKSDVYSYGMLVLDLVLGGIRNPNSSLLSDESELYFPNWVFKKFET- 251
           APEVFS  +G VS+KSDVYSYGMLVL+++    +    +  S+ S  YFP+W++K  E  
Sbjct: 489 APEVFSGMYGRVSHKSDVYSYGMLVLEMIGAKNKEIEETAASNSSSAYFPDWIYKNLENG 548

Query: 252 DKTTRMDQNLLEEDEEMERKMVMIGLWCIQTSPLDRPSMSRVLEMLEGSIHSLQMPPRPS 310
           + T +    +  ED+E+ +KM ++GLWCIQ SPL+RP M+R++EM+EGS+  L++PP+PS
Sbjct: 549 EDTWKFGDEISREDKEVAKKMTLVGLWCIQPSPLNRPPMNRIVEMMEGSLDVLEVPPKPS 608

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_011654383.14.5e-14882.01LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 [Cuc... [more]
QKK82668.15.8e-14882.01LRK10L2 [Cucumis sativus][more]
KAE8649647.11.1e-13382.06hypothetical protein Csa_012280 [Cucumis sativus][more]
KAA8547784.11.6e-10862.15hypothetical protein F0562_004213 [Nyssa sinensis][more]
KAF3955879.11.0e-10759.76hypothetical protein CMV_018949 [Castanea mollissima][more]
Match NameE-valueIdentityDescription
F4HQ222.9e-10259.08LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 OS=A... [more]
D7SFH91.0e-9957.89Protein SUPPRESSOR OF NPR1-1 CONSTITUTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=L... [more]
Q3ECH22.6e-9855.93LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.8 OS=A... [more]
Q9FF313.4e-9857.14LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 OS=A... [more]
F4HQ201.3e-9756.00LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.5 OS=A... [more]
Match NameE-valueIdentityDescription
A0A6M9BN162.8e-14882.01LRK10L2 OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A2N9I4J74.3e-11262.92Protein kinase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCU... [more]
A0A2N9FST59.5e-11262.61Protein kinase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCU... [more]
A0A5B7C1384.0e-11064.59Protein kinase domain-containing protein (Fragment) OS=Davidia involucrata OX=16... [more]
A0A5J5BYQ67.5e-10962.15Protein kinase domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_00... [more]
Match NameE-valueIdentityDescription
AT1G66920.12.1e-10359.08Protein kinase superfamily protein [more]
AT1G66920.22.1e-10359.08Protein kinase superfamily protein [more]
AT1G66980.17.4e-10157.89suppressor of npr1-1 constitutive 4 [more]
AT1G67000.11.8e-9955.93Protein kinase superfamily protein [more]
AT5G38260.12.4e-9957.14Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 24..294
e-value: 9.9E-34
score: 128.1
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 27..292
e-value: 2.3E-48
score: 164.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 24..302
score: 38.067822
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 99..304
e-value: 6.4E-49
score: 168.1
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 2..98
e-value: 3.6E-26
score: 93.3
NoneNo IPR availablePIRSFPIRSF000654ILKcoord: 3..304
e-value: 2.2E-33
score: 113.5
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 30..218
e-value: 1.2E-23
score: 81.8
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 30..218
e-value: 1.2E-23
score: 81.8
NoneNo IPR availablePIRSRPIRSR000556-1PIRSR000556-1coord: 25..221
e-value: 1.2E-23
score: 81.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..329
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 302..329
NoneNo IPR availablePANTHERPTHR27009RUST RESISTANCE KINASE LR10-RELATEDcoord: 8..325
NoneNo IPR availablePANTHERPTHR27009:SF223SUBFAMILY NOT NAMEDcoord: 8..325
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 141..153
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 30..52
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 6..295

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0000288.1Lag0000288.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0030247 polysaccharide binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity