Lag0017281 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0017281
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDolichol phosphate-mannose biosynthesis regulatory protein
Locationchr5: 1714358 .. 1717205 (-)
RNA-Seq ExpressionLag0017281
SyntenyLag0017281
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGACAGAAGCGTAATTTGCAATCTTTTGAGACCTAAGCAAAAAGTCCAACATTTTTTATTTTCCTACAAGGGTTAAAAATCGAATTTAGCCTTCCAAATTGGTTCATTCTTCGCAAAAACCCTTTCAGATTTTTGCCTCATTCCTCAATCCTTCCAACATTTTCAGCTCCGATTATCTTCATCTTCGACCAGAATTCCGGCGACGGCGAATCAGGTATGAGCTCTATACCACGCTATCGGTAACGCATCGATTAACTCTTGCTGCAAAAGTTTCGGCGATCTTTGATCTTTGTTATGGTTTTTTTTGGGCGAATTCGTTGATTCCATTTGAAAATATAAGTTCTACACTTATTGTTCTGTAATTACAGGAATTGATTTGAATTTATAAGGTGGCACTGTGATTAGTTTGAATGATGTTGGCTGGCTGCATCTGTTTACTAATCTTCAGCTCCATTAGCTTCAATTATTTCACATTTCTTATTAAAAAATGTATTGAAGATTAACAGGACTATTCGTGAGTACGTGAAAACTAGAATTATGTGCGGATGAACCTATTGGTGAACTTAAACCCAGAAATGAGAATAGTAAAAAACAAAAACTTAAGACGTTTAGGTTTTGTAATGAAGATTGTTATAGTTTCTTTCTGATTAGATTGCTTCATGACATCATAGGCAATTTGCAGTTTTTGTTCTCGAATTCAGTAGTTGTCTCTGAGACGCAGATGGTATTGGCTATTGAATACTTTTCCTTCATTATTTAGTAATTATGAGCAAATTTGTTAAATGGTGACAGATACCTAAAAAATCCTAACATAACCTTAAATCTTTAGTTTTATTTCAAAATTCAAGTATCAGGTTTTGAAGCAGTCCCTTTGTATTGCTTACGTTTTAAAGTCTCCATGAATACTAACTAGGCAGAACAAAAAGGACTTTAACGTTACCTCTCTCTTCTACCTCTTAAGAATTTGGATTCTTCCCCTTTGCACGTCAATAGTTCCTTGTCTTCCTCTTTCAATTACAGAATTCTTCCATGCCTTTTTCTTACATTTATAAACATCAAAACCCATCTTCATGTTTAAAACGTATTTCCTAGGTATCTATGGTTCCAGTGGGCTGATCTCAAGTCTTTTGGAATCCATTTCAGGTCCCCCATGGCAGTGCAAGTGCATTTGTGAGACTTTATTAGATGTTCTGTTTTGCAAATTTTGAAATTTGATTTTCACACCTGGATCTTGTACTGAAATCAGATCTTCCTTGGAAGGTTATGAAACTTTTGCTCTGGGTTTCTAGGCTCTTATTCCCTTGAAGCTTGATTTGTATTCTAAAGATTATGAATTATCCGCCATCCTTATGGTGCATTTTCTACAATTCACTTATAGGAATAGAATTGTGAGAATCAATTATAATCTAATTCAACCACATGGCTCGGAATTGCTCACATGGCGTTCCTCTTGGTTTGTACAATATTTTTTTTTAACTGAAAAACAAACCACTTTCATTTAGAAAAATGAAAGAACATACCAGGGTGATATGAAACTTGGACCAAAGGTCAAGTAGAAATTTCTAGCTGTTTAGTGATGTCTGAACATCATCTAGATGTGCATTTCTTCTCCCAAGTGAAAACAAAAGATGGAAAAGAAAGTCATGTGAGGTTGATGGAGTTAGTTCCGGTTGATTATTTATTTGTACAGTCTATTAGCACAAGGATGCACATATATCTATAATTGTATAATATGTTGATCATATATCAGTCTTTTTAAAAGGTCATACCAATCCCAGAATTTTCTTTTTTCTTGATAAAAGGGATGGTTTTATTAGTATCTATGCACAAGATCAAACTGAACCGTGATTATTTATTCAACAATCGAGTTTAAATCTAATTTTTGCACTTGAATGATTTGTCGAGATTAATCATCTACGAGAATTTCTGTGAACTTTTTGATGAGATTAGCAGACAGTGCTAATAAGGGTGAGGTTTCATCTTGTCGTGAACTCTTTCTAGTTGTCGACTGAATTCTTATATAACATGCCAAACTACTACAACATATGATGATAAATGTTGTGCCATATGGAACGTAGTAATCTCTATATAGAGCAGGATAAGTTTGGACGTAGTTCATTTGGCACTCTTCACTTTAAATTATGTTTGGAAGTAATATAATGTGCTTTGGAAAAAAAATTCAAAAGAACCATAAATTGCTTTATGAGGTAGAGTGTTTTGGGTGCAAATGATTAATCAAGAAGCACATGGAAGTATTTTTATTTTTTTTTTTAATTTTTTTTGAGTACATCAACATTTGGGGGTGGGGGGATTCGAACCTCTGACCTCATGGTCGAGGATACATGCCAACTACCGTTGAGCTAAGCTCGCTTTTGCGTATTTTTAAGAGGGTAGAACCAAATATCAAAATGATCTGTTATTAAGTACTTAGTATTAAGGTGCTTATAACTAAAATACTTGATACTGATGTGCAGTGTACATTATTCACAGGAAATAATAGCATCAAGATGGAGTTAGCAGACAAGACTGTTGGATTTTTGTTGACGCTAACCAGCTTGTCTATATTCACTTACTATACTTTCTGGGTCATTATCCTGGTAAGCTTATCTTCAATGTGATCTCATTGAGTGAACTTGATCATATTCATGATGGGTTTTATCAACCAATGTATTTTGTTTTGATTAATGGCAGCCATTTGTAGATAGTGATCACTTCATCCACAGCTATTTCTTACCACAAGAATATGCCATCCTGATACCTGTATTTGCTGGTGTGGTACTTCTGTGCTTATTAGGCATATTTATAGGAATGGTGATGCTCAAATCCAAAAAGAAGAAGGCATGA

mRNA sequence

ATGAGGACAGAAGCATTTTTGCCTCATTCCTCAATCCTTCCAACATTTTCAGCTCCGATTATCTTCATCTTCGACCAGAATTCCGGCGACGGCGAATCAGGTATGAGCTCTATACCACGCTATCGATTGCTTCATGACATCATAGGCAATTTGCAGTTTTTGTTCTCGAATTCAGTAGTTGTCTCTGAGACGCAGATGGCTCTTATTCCCTTGAAGCTTGATTTGTATTCTAAAGATTATGAATTATCCGCCATCCTTATGTGTACATTATTCACAGGAAATAATAGCATCAAGATGGAGTTAGCAGACAAGACTGTTGGATTTTTGTTGACGCTAACCAGCTTGTCTATATTCACTTACTATACTTTCTGGGTCATTATCCTGCCATTTGTAGATAGTGATCACTTCATCCACAGCTATTTCTTACCACAAGAATATGCCATCCTGATACCTGTATTTGCTGGTGTGGTACTTCTGTGCTTATTAGGCATATTTATAGGAATGGTGATGCTCAAATCCAAAAAGAAGAAGGCATGA

Coding sequence (CDS)

ATGAGGACAGAAGCATTTTTGCCTCATTCCTCAATCCTTCCAACATTTTCAGCTCCGATTATCTTCATCTTCGACCAGAATTCCGGCGACGGCGAATCAGGTATGAGCTCTATACCACGCTATCGATTGCTTCATGACATCATAGGCAATTTGCAGTTTTTGTTCTCGAATTCAGTAGTTGTCTCTGAGACGCAGATGGCTCTTATTCCCTTGAAGCTTGATTTGTATTCTAAAGATTATGAATTATCCGCCATCCTTATGTGTACATTATTCACAGGAAATAATAGCATCAAGATGGAGTTAGCAGACAAGACTGTTGGATTTTTGTTGACGCTAACCAGCTTGTCTATATTCACTTACTATACTTTCTGGGTCATTATCCTGCCATTTGTAGATAGTGATCACTTCATCCACAGCTATTTCTTACCACAAGAATATGCCATCCTGATACCTGTATTTGCTGGTGTGGTACTTCTGTGCTTATTAGGCATATTTATAGGAATGGTGATGCTCAAATCCAAAAAGAAGAAGGCATGA

Protein sequence

MRTEAFLPHSSILPTFSAPIIFIFDQNSGDGESGMSSIPRYRLLHDIIGNLQFLFSNSVVVSETQMALIPLKLDLYSKDYELSAILMCTLFTGNNSIKMELADKTVGFLLTLTSLSIFTYYTFWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVLLCLLGIFIGMVMLKSKKKKA
Homology
[Close]
BLAST of Lag0017281 vs. NCBI nr
Match: KAG6596723.1 (Binding partner of ACD11 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 161.8 bits (408), Expect = 5.6e-36
Identity = 99/176 (56.25%), Postives = 106/176 (60.23%), Query Frame = 0 

Query: 6   FLPHSSILPTFSAPIIFI---FDQNSGDGESGMSSIPRYRLLHDIIGNLQFLFSNSVVVS 65
           FLPHS ILP FS PI FI     QNSGD +                              
Sbjct: 352 FLPHSLILPIFSPPITFIVRSITQNSGDDDE----------------------------- 411

Query: 66  ETQMALIPLKLDLYSKDYELSAILMCTLFTGNNSIKMELADKTVGFLLTLTSLSIFTYYT 125
                                        +G+NSIKMELADKTVGFLL+LTSLSIFTYYT
Sbjct: 412 -----------------------------SGSNSIKMELADKTVGFLLSLTSLSIFTYYT 469

Query: 126 FWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVLLCLLGIFIGMVMLKSKKKKA 179
           FWVIILPFVDSDHFIH+YFLPQEYAILIPVFAGVVLLCLL IF+G+VMLKSKKKKA
Sbjct: 472 FWVIILPFVDSDHFIHNYFLPQEYAILIPVFAGVVLLCLLAIFVGIVMLKSKKKKA 469

[Close]
BLAST of Lag0017281 vs. NCBI nr
Match: KAG7028258.1 (Dolichol-phosphate mannose synthase subunit 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 161.8 bits (408), Expect = 5.6e-36
Identity = 99/176 (56.25%), Postives = 106/176 (60.23%), Query Frame = 0 

Query: 6   FLPHSSILPTFSAPIIFI---FDQNSGDGESGMSSIPRYRLLHDIIGNLQFLFSNSVVVS 65
           FLPHS ILP FS PI FI     QNSGD +                              
Sbjct: 6   FLPHSLILPIFSPPITFIVRSITQNSGDDDE----------------------------- 65

Query: 66  ETQMALIPLKLDLYSKDYELSAILMCTLFTGNNSIKMELADKTVGFLLTLTSLSIFTYYT 125
                                        +G+NSIKMELADKTVGFLL+LTSLSIFTYYT
Sbjct: 66  -----------------------------SGSNSIKMELADKTVGFLLSLTSLSIFTYYT 123

Query: 126 FWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVLLCLLGIFIGMVMLKSKKKKA 179
           FWVIILPFVDSDHFIH+YFLPQEYAILIPVFAGVVLLCLL IF+G+VMLKSKKKKA
Sbjct: 126 FWVIILPFVDSDHFIHNYFLPQEYAILIPVFAGVVLLCLLAIFVGIVMLKSKKKKA 123

[Close]
BLAST of Lag0017281 vs. NCBI nr
Match: XP_038904650.1 (dolichol-phosphate mannose synthase subunit 2 [Benincasa hispida] >XP_038904651.1 dolichol-phosphate mannose synthase subunit 2 [Benincasa hispida] >XP_038904652.1 dolichol-phosphate mannose synthase subunit 2 [Benincasa hispida] >XP_038904653.1 dolichol-phosphate mannose synthase subunit 2 [Benincasa hispida] >XP_038904654.1 dolichol-phosphate mannose synthase subunit 2 [Benincasa hispida])

HSP 1 Score: 147.5 bits (371), Expect = 1.1e-31
Identity = 75/80 (93.75%), Postives = 79/80 (98.75%), Query Frame = 0 

Query: 99  MELADKTVGFLLTLTSLSIFTYYTFWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVL 158
           MELADKTVGFLL+LTSLSIFTYYTFWVIILPFVDSDHFIH+YFLPQEYAILIP+FAGVVL
Sbjct: 1   MELADKTVGFLLSLTSLSIFTYYTFWVIILPFVDSDHFIHNYFLPQEYAILIPIFAGVVL 60

Query: 159 LCLLGIFIGMVMLKSKKKKA 179
           LC LGIF+GMVMLKSKKKKA
Sbjct: 61  LCFLGIFVGMVMLKSKKKKA 80

[Close]
BLAST of Lag0017281 vs. NCBI nr
Match: XP_004148753.1 (dolichol-phosphate mannose synthase subunit 2 [Cucumis sativus] >KGN55729.1 hypothetical protein Csa_010385 [Cucumis sativus])

HSP 1 Score: 146.7 bits (369), Expect = 1.8e-31
Identity = 75/80 (93.75%), Postives = 79/80 (98.75%), Query Frame = 0 

Query: 99  MELADKTVGFLLTLTSLSIFTYYTFWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVL 158
           MELADKTVGFLL+LTSLSIFTYYTFWV+ILPFVDSDHFIH+YFLPQEYAILIPVFAGVVL
Sbjct: 1   MELADKTVGFLLSLTSLSIFTYYTFWVVILPFVDSDHFIHNYFLPQEYAILIPVFAGVVL 60

Query: 159 LCLLGIFIGMVMLKSKKKKA 179
           LCLLGIF+G VMLKSKKKKA
Sbjct: 61  LCLLGIFVGTVMLKSKKKKA 80

[Close]
BLAST of Lag0017281 vs. NCBI nr
Match: XP_008448655.2 (PREDICTED: dolichol phosphate-mannose biosynthesis regulatory protein [Cucumis melo])

HSP 1 Score: 146.4 bits (368), Expect = 2.4e-31
Identity = 74/80 (92.50%), Postives = 79/80 (98.75%), Query Frame = 0 

Query: 99  MELADKTVGFLLTLTSLSIFTYYTFWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVL 158
           MELADKTVGFLL+LTSLSIFTYYTFWV+ILPFVDSDHF+H+YFLPQEYAILIPVFAGVVL
Sbjct: 1   MELADKTVGFLLSLTSLSIFTYYTFWVVILPFVDSDHFVHNYFLPQEYAILIPVFAGVVL 60

Query: 159 LCLLGIFIGMVMLKSKKKKA 179
           LCLLGIF+G VMLKSKKKKA
Sbjct: 61  LCLLGIFVGTVMLKSKKKKA 80

[Close]
BLAST of Lag0017281 vs. ExPASy Swiss-Prot
Match: Q9CA79 (Dolichol-phosphate mannose synthase subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DPMS2 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 6.8e-29
Identity = 65/80 (81.25%), Postives = 72/80 (90.00%), Query Frame = 0 

Query: 99  MELADKTVGFLLTLTSLSIFTYYTFWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVL 158
           MELAD+ VG LL+  SLSIFTYYTFWVIILPFVDSDHFIH YFLPQ+YAIL+PVFAG+ L
Sbjct: 1   MELADRAVGLLLSSISLSIFTYYTFWVIILPFVDSDHFIHKYFLPQDYAILVPVFAGIAL 60

Query: 159 LCLLGIFIGMVMLKSKKKKA 179
           L L+ +FIGMVMLKSKKKKA
Sbjct: 61  LSLISVFIGMVMLKSKKKKA 80

[Close]
BLAST of Lag0017281 vs. ExPASy Swiss-Prot
Match: Q9Z324 (Dolichol phosphate-mannose biosynthesis regulatory protein OS=Mus musculus OX=10090 GN=Dpm2 PE=3 SV=3)

HSP 1 Score: 89.4 bits (220), Expect = 4.6e-17
Identity = 48/78 (61.54%), Postives = 60/78 (76.92%), Query Frame = 0 

Query: 103 DKTVGFLLTLTSLSIFTYYTFWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVLLCLL 162
           D+ VGF L   SL IFTYYT WVI+LPF+DS H IH YFLP+ YA+L+P+ AG++LL  +
Sbjct: 6   DQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLLLLLFV 65

Query: 163 GIFIGMVMLKSKK--KKA 179
           G+FI  VMLKS+K  KKA
Sbjct: 66  GLFITYVMLKSQKITKKA 83

[Close]
BLAST of Lag0017281 vs. ExPASy Swiss-Prot
Match: Q9Z325 (Dolichol phosphate-mannose biosynthesis regulatory protein OS=Rattus norvegicus OX=10116 GN=Dpm2 PE=1 SV=3)

HSP 1 Score: 88.2 bits (217), Expect = 1.0e-16
Identity = 47/78 (60.26%), Postives = 60/78 (76.92%), Query Frame = 0 

Query: 103 DKTVGFLLTLTSLSIFTYYTFWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVLLCLL 162
           D+ VGF L   SL IFTYYT WVI+LPF+DS H IH YFLP+ YA+L+P+ AG++LL  +
Sbjct: 6   DQAVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLLPLAAGLLLLLFV 65

Query: 163 GIFIGMVMLKSKK--KKA 179
           G+FI  V+LKS+K  KKA
Sbjct: 66  GLFITYVLLKSQKVTKKA 83

[Close]
BLAST of Lag0017281 vs. ExPASy Swiss-Prot
Match: Q9Z1P1 (Dolichol phosphate-mannose biosynthesis regulatory protein OS=Cricetulus griseus OX=10029 GN=DPM2 PE=3 SV=3)

HSP 1 Score: 87.8 bits (216), Expect = 1.3e-16
Identity = 47/78 (60.26%), Postives = 59/78 (75.64%), Query Frame = 0 

Query: 103 DKTVGFLLTLTSLSIFTYYTFWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVLLCLL 162
           D+ VGF L   SL IFTYYT WVI+LPF+DS H IH YFLP+ YA+LIP+  G++LL  +
Sbjct: 6   DQVVGFGLVAVSLIIFTYYTTWVILLPFIDSQHVIHKYFLPRAYAVLIPLATGLLLLLFV 65

Query: 163 GIFIGMVMLKSKK--KKA 179
           G+FI  VMLKS++  KKA
Sbjct: 66  GLFITYVMLKSRRLTKKA 83

[Close]
BLAST of Lag0017281 vs. ExPASy Swiss-Prot
Match: O94777 (Dolichol phosphate-mannose biosynthesis regulatory protein OS=Homo sapiens OX=9606 GN=DPM2 PE=1 SV=3)

HSP 1 Score: 85.9 bits (211), Expect = 5.1e-16
Identity = 46/78 (58.97%), Postives = 58/78 (74.36%), Query Frame = 0 

Query: 103 DKTVGFLLTLTSLSIFTYYTFWVIILPFVDSDHFIHSYFLPQEYAILIPVFAGVVLLCLL 162
           D+ VG  L   SL IFTYYT WVI+LPF+DS H IH YFLP+ YA+ IP+ AG++LL  +
Sbjct: 6   DQVVGLGLVAVSLIIFTYYTAWVILLPFIDSQHVIHKYFLPRAYAVAIPLAAGLLLLLFV 65

Query: 163 GIFIGMVMLKSKK--KKA 179
           G+FI  VMLK+K+  KKA
Sbjct: 66  GLFISYVMLKTKRVTKKA 83

The following BLAST results are available for this feature:
BLAST of Lag0017281 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6596723.15.6e-3656.25Binding partner of ACD11 1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7028258.15.6e-3656.25Dolichol-phosphate mannose synthase subunit 2, partial [Cucurbita argyrosperma s... [more]
XP_038904650.11.1e-3193.75dolichol-phosphate mannose synthase subunit 2 [Benincasa hispida] >XP_038904651.... [more]
XP_004148753.11.8e-3193.75dolichol-phosphate mannose synthase subunit 2 [Cucumis sativus] >KGN55729.1 hypo... [more]
XP_008448655.22.4e-3192.50PREDICTED: dolichol phosphate-mannose biosynthesis regulatory protein [Cucumis m... [more]
BLAST of Lag0017281 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9CA796.8e-2981.25Dolichol-phosphate mannose synthase subunit 2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9Z3244.6e-1761.54Dolichol phosphate-mannose biosynthesis regulatory protein OS=Mus musculus OX=10... [more]
Q9Z3251.0e-1660.26Dolichol phosphate-mannose biosynthesis regulatory protein OS=Rattus norvegicus ... [more]
Q9Z1P11.3e-1660.26Dolichol phosphate-mannose biosynthesis regulatory protein OS=Cricetulus griseus... [more]
O947775.1e-1658.97Dolichol phosphate-mannose biosynthesis regulatory protein OS=Homo sapiens OX=96... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009914Dolichol phosphate-mannose biosynthesis regulatoryPFAMPF07297DPM2coord: 102..177
e-value: 3.0E-35
score: 120.3
IPR009914Dolichol phosphate-mannose biosynthesis regulatoryPANTHERPTHR15039DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEINcoord: 74..178
NoneNo IPR availablePANTHERPTHR15039:SF12DOLICHOL PHOSPHATE-MANNOSE BIOSYNTHESIS REGULATORY PROTEINcoord: 74..178

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0017281.1Lag0017281.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019348 dolichol metabolic process
biological_process GO:0006506 GPI anchor biosynthetic process
biological_process GO:0006486 protein glycosylation
biological_process GO:0050790 regulation of catalytic activity
cellular_component GO:0033185 dolichol-phosphate-mannose synthase complex
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane
molecular_function GO:0030234 enzyme regulator activity