Lag0041665 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0041665
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	GDSL esterase/lipase 5-like
Location	chr13: 23525008 .. 23525634 (+)
RNA-Seq Expression	Lag0041665
Synteny	Lag0041665

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGGAGATGAGAATGGAAGTTGTTTGGATGAAGCCTCATTAGTATCAAGGCTCAACAACAAACTCATGCCCATTGCCCTAAACAACCTTGCCATTCAACTCAAAGGCTTCAAATACACCATCTTTGATTCCTACACTATGCTTCTCCAAAGAATGGAAAACCCTTCAAAATATGGTAACCAAATTCCCCTATACTTTTCTTTGTATACTTTCTTTTTTTAGTACAAAAAACAATGGTATGAAGTGTATGATTTTGAGTCAAACATCCCTGACATTTAGAAAACTTTCTTTCATATTTAAATTATATTAAGTTATTAATAGTATAGTATACTTTAACTCAAAAGAAGTTATGGTGTGTTGGATGCAGGTTTCAAGGAAGGGATAACAGCATGCTGTGGAAGTGAAAGGTTTAGAGGAGTGTATAGTTGTGGAGGGAAAAGATTAGTGGAAGAGTACGAACTGTGCGAAAATCCCAACGACTATTTGTTTTTTGATTCCTTTCATCTTACTGAAAAAGGGTACGAGCAATTTGCAAAGTTGATGTGGAGTGGAGATGCTAAAGTCATGAAGCCTTATAACCTCAAACAACTCTTCGAATATGAAACCTTGTTGGCTTCTGAGTGA

mRNA sequence

ATGAAAGGAGATGAGAATGGAAGTTGTTTGGATGAAGCCTCATTAGTATCAAGGCTCAACAACAAACTCATGCCCATTGCCCTAAACAACCTTGCCATTCAACTCAAAGGCTTCAAATACACCATCTTTGATTCCTACACTATGCTTCTCCAAAGAATGGAAAACCCTTCAAAATATGGTTTCAAGGAAGGGATAACAGCATGCTGTGGAAGTGAAAGGTTTAGAGGAGTGTATAGTTGTGGAGGGAAAAGATTAGTGGAAGAGTACGAACTGTGCGAAAATCCCAACGACTATTTGTTTTTTGATTCCTTTCATCTTACTGAAAAAGGGTACGAGCAATTTGCAAAGTTGATGTGGAGTGGAGATGCTAAAGTCATGAAGCCTTATAACCTCAAACAACTCTTCGAATATGAAACCTTGTTGGCTTCTGAGTGA

Coding sequence (CDS)

ATGAAAGGAGATGAGAATGGAAGTTGTTTGGATGAAGCCTCATTAGTATCAAGGCTCAACAACAAACTCATGCCCATTGCCCTAAACAACCTTGCCATTCAACTCAAAGGCTTCAAATACACCATCTTTGATTCCTACACTATGCTTCTCCAAAGAATGGAAAACCCTTCAAAATATGGTTTCAAGGAAGGGATAACAGCATGCTGTGGAAGTGAAAGGTTTAGAGGAGTGTATAGTTGTGGAGGGAAAAGATTAGTGGAAGAGTACGAACTGTGCGAAAATCCCAACGACTATTTGTTTTTTGATTCCTTTCATCTTACTGAAAAAGGGTACGAGCAATTTGCAAAGTTGATGTGGAGTGGAGATGCTAAAGTCATGAAGCCTTATAACCTCAAACAACTCTTCGAATATGAAACCTTGTTGGCTTCTGAGTGA

Protein sequence

MKGDENGSCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYGFKEGITACCGSERFRGVYSCGGKRLVEEYELCENPNDYLFFDSFHLTEKGYEQFAKLMWSGDAKVMKPYNLKQLFEYETLLASE

Homology

[Close]

BLAST of Lag0041665 vs. NCBI nr
Match: XP_038897092.1 (GDSL lipase-like [Benincasa hispida])

HSP 1 Score: 206.8 bits (525), Expect = 1.2e-49
Identity = 94/136 (69.12%), Postives = 114/136 (83.82%), Query Frame = 0


Query: 1   MKGDENGSCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYG 60
           MK   +GSC DEAS ++RL+NKL+PIAL  LA +L+GFKYT+ D+YT+L  R++NPSKYG
Sbjct: 232 MKKGGHGSCWDEASALTRLHNKLLPIALQKLADKLQGFKYTVADTYTLLQNRIDNPSKYG 291

Query: 61  FKEGITACCGSERFRGVYSCGGKRLVEEYELCENPNDYLFFDSFHLTEKGYEQFAKLMWS 120
           FKEG  ACCGS   RG+YSCGG R  +E+ELCENPN+YLFFDS+H  EK YEQFAKLMWS
Sbjct: 292 FKEGKKACCGSGELRGIYSCGGMRGQKEFELCENPNEYLFFDSYHPNEKAYEQFAKLMWS 351

Query: 121 GDAKVMKPYNLKQLFE 137
           GDA+V+KPYNLKQLF+
Sbjct: 352 GDAQVIKPYNLKQLFQ 367

[Close]

BLAST of Lag0041665 vs. NCBI nr
Match: XP_008462253.1 (PREDICTED: GDSL esterase/lipase 5-like isoform X1 [Cucumis melo] >KAA0059365.1 GDSL esterase/lipase 5-like isoform X1 [Cucumis melo var. makuwa] >TYK03961.1 GDSL esterase/lipase 5-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 203.4 bits (516), Expect = 1.3e-48
Identity = 93/137 (67.88%), Postives = 112/137 (81.75%), Query Frame = 0


Query: 1   MKGDENGSCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYG 60
           MK D +GSCLDEAS + RL+NKL+  AL  LA +L GFKYTI D+YTML  R++NPSKYG
Sbjct: 232 MKKDGHGSCLDEASALVRLHNKLLHAALQKLADKLHGFKYTIGDTYTMLQNRIDNPSKYG 291

Query: 61  FKEGITACCGSERFRGVYSCGGKRLVEEYELCENPNDYLFFDSFHLTEKGYEQFAKLMWS 120
            KEG  ACCGS + RG+YSCGG R V+E+ELCENPN+YLFFDS+H  E+ YEQFAKLMWS
Sbjct: 292 LKEGKKACCGSGKLRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWS 351

Query: 121 GDAKVMKPYNLKQLFEY 138
           GD++V+ PYNLKQ F+Y
Sbjct: 352 GDSQVINPYNLKQFFQY 368

[Close]

BLAST of Lag0041665 vs. NCBI nr
Match: XP_022146472.1 (GDSL esterase/lipase 1-like [Momordica charantia])

HSP 1 Score: 202.6 bits (514), Expect = 2.3e-48
Identity = 87/138 (63.04%), Postives = 115/138 (83.33%), Query Frame = 0


Query: 2   KGDENGSCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYGF 61
           +GD +G C +EAS ++RL+NK++P AL  LAI+LKGFKYT+ D+YT+L  R++NPSKYGF
Sbjct: 234 RGDRHGRCWEEASALARLHNKMLPGALKKLAIKLKGFKYTVADTYTLLQNRIDNPSKYGF 293

Query: 62  KEGITACCGSERFRGVYSCGGKRLVEEYELCENPNDYLFFDSFHLTEKGYEQFAKLMWSG 121
           KEG + CCGS  +RG+YSCGGKR  +E+ELC+NPN YLFFDS+H  ++ YEQFAK MWSG
Sbjct: 294 KEGKSGCCGSGEYRGIYSCGGKRGQKEFELCKNPNQYLFFDSYHPNQRAYEQFAKHMWSG 353

Query: 122 DAKVMKPYNLKQLFEYET 140
           D +V+ PYNLKQLF+++T
Sbjct: 354 DTQVINPYNLKQLFQHDT 371

[Close]

BLAST of Lag0041665 vs. NCBI nr
Match: XP_004141726.1 (GDSL lipase isoform X1 [Cucumis sativus] >KGN45451.1 hypothetical protein Csa_016520 [Cucumis sativus])

HSP 1 Score: 199.5 bits (506), Expect = 1.9e-47
Identity = 90/136 (66.18%), Postives = 112/136 (82.35%), Query Frame = 0


Query: 2   KGDENGSCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYGF 61
           K   +GSC DE S + RL+NKL+P AL  LA +L+GFKYT+ D+YTML  R++NPSKYGF
Sbjct: 234 KAGGHGSCWDEPSALVRLHNKLLPGALQKLADKLQGFKYTVGDTYTMLQNRIDNPSKYGF 293

Query: 62  KEGITACCGSERFRGVYSCGGKRLVEEYELCENPNDYLFFDSFHLTEKGYEQFAKLMWSG 121
           KE  TACCGS +FRG+YSCGG R V+E+ELCENPN+YLFFDS+H  E+ YEQFAKLMWSG
Sbjct: 294 KEEKTACCGSGKFRGIYSCGGMRGVKEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSG 353

Query: 122 DAKVMKPYNLKQLFEY 138
           D++V+ PY+LKQ F+Y
Sbjct: 354 DSQVINPYSLKQFFQY 369

[Close]

BLAST of Lag0041665 vs. NCBI nr
Match: XP_008462251.1 (PREDICTED: GDSL esterase/lipase 5-like [Cucumis melo] >KAA0059366.1 GDSL esterase/lipase 5-like [Cucumis melo var. makuwa] >TYK03960.1 GDSL esterase/lipase 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 197.6 bits (501), Expect = 7.4e-47
Identity = 89/137 (64.96%), Postives = 109/137 (79.56%), Query Frame = 0


Query: 6   NGSCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYGFKEGI 65
           +GSC DEAS ++ L+NKL PIAL   A +  GFKYT+ D YTML  R++NPSKYGFKEG 
Sbjct: 244 DGSCWDEASALATLHNKLFPIALQKFADKFPGFKYTVADMYTMLQNRIDNPSKYGFKEGK 303

Query: 66  TACCGSERFRGVYSCGGKRLVEEYELCENPNDYLFFDSFHLTEKGYEQFAKLMWSGDAKV 125
            ACCGS +FRG++SCG  R  EE+ELCENPN+YLFFDS+H  E+ YEQFAKLMWSGD++V
Sbjct: 304 KACCGSGKFRGIFSCGRMRGEEEFELCENPNEYLFFDSYHPNERAYEQFAKLMWSGDSQV 363

Query: 126 MKPYNLKQLFEYETLLA 143
           + PYNLKQ F+Y +L A
Sbjct: 364 INPYNLKQFFQYGSLQA 380

[Close]

BLAST of Lag0041665 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 153.7 bits (387), Expect = 1.6e-36
Identity = 68/133 (51.13%), Postives = 99/133 (74.44%), Query Frame = 0


Query: 8   SCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYGFKEGITA 67
           SCL +AS ++ ++N+ +   L  +  Q+KGFK+++FD    L  RM++PSK+GFKEG  A
Sbjct: 251 SCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKEGEEA 310

Query: 68  CCGSERFRGVYSCGGKRLVEEYELCENPNDYLFFDSFHLTEKGYEQFAKLMWSG----DA 127
           CCG+ ++RGV+SCGGKR+V+EY+LCENP DY+F+DS HLT+  Y QFA L+W+G    D+
Sbjct: 311 CCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGHMSDS 370

Query: 128 KVMKPYNLKQLFE 137
            V+ PYN+  LF+
Sbjct: 371 LVVGPYNINNLFQ 383

[Close]

BLAST of Lag0041665 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 9.7e-34
Identity = 68/131 (51.91%), Postives = 87/131 (66.41%), Query Frame = 0


Query: 7   GSCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYGFKEGIT 66
           G+C    + +  L+N+ +   L  L  +L GFKY + D +T L  RM NPSKYGFKEG  
Sbjct: 244 GTCFKPVTELINLHNEKLESGLRRLERELSGFKYALHDYHTSLSVRMNNPSKYGFKEGKM 303

Query: 67  ACCGSERFRGVYSCGGKRLV-EEYELCENPNDYLFFDSFHLTEKGYEQFAKLMWSGDAKV 126
           ACCG+   RG+ +CGG+  V + YELCE   DYLFFD FHLTEK ++Q A+L+WSG   V
Sbjct: 304 ACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFFDHFHLTEKAHQQIAELIWSGPTNV 363

Query: 127 MKPYNLKQLFE 137
            KPYNL+ LFE
Sbjct: 364 TKPYNLQALFE 374

[Close]

BLAST of Lag0041665 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 8.2e-33
Identity = 67/130 (51.54%), Postives = 85/130 (65.38%), Query Frame = 0


Query: 8   SCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYGFKEGITA 67
           SC    + +  ++N+ +   L  L  +L GFKY + D +T L +RM +PSKYGFKEG  A
Sbjct: 243 SCFQPVTELINMHNEKLLNGLRRLNHELSGFKYALHDYHTSLSERMNDPSKYGFKEGKKA 302

Query: 68  CCGSERFRGVYSCGGKR-LVEEYELCENPNDYLFFDSFHLTEKGYEQFAKLMWSGDAKVM 127
           CCGS   RG+ +CGG+  L + YELCEN  DYLFFD FHLTEK   Q A+L+WSG   + 
Sbjct: 303 CCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFFDPFHLTEKANRQIAELIWSGPTNIT 362

Query: 128 KPYNLKQLFE 137
            PYNLK LFE
Sbjct: 363 GPYNLKALFE 372

[Close]

BLAST of Lag0041665 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 136.7 bits (343), Expect = 2.0e-31
Identity = 65/131 (49.62%), Postives = 85/131 (64.89%), Query Frame = 0


Query: 7   GSCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYGFKEGIT 66
           GSC    + +  ++NK  P  L  L  QL GF+Y + D +T L +R+ +PSKYGFKEG  
Sbjct: 233 GSCFKPVAELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGKK 292

Query: 67  ACCGSERFRGVYSCGGK-RLVEEYELCENPNDYLFFDSFHLTEKGYEQFAKLMWSGDAKV 126
           ACCGS   RG+ +CG +    + Y LCEN  DYLF+DS HLTEK + Q A+L+W+G   V
Sbjct: 293 ACCGSGPLRGINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPNV 352

Query: 127 MKPYNLKQLFE 137
            +PYNLK LFE
Sbjct: 353 TRPYNLKALFE 363

[Close]

BLAST of Lag0041665 vs. ExPASy Swiss-Prot
Match: Q9LJP1 (GDSL esterase/lipase 4 OS=Arabidopsis thaliana OX=3702 GN=GLIP4 PE=2 SV=2)

HSP 1 Score: 135.6 bits (340), Expect = 4.5e-31
Identity = 64/130 (49.23%), Postives = 85/130 (65.38%), Query Frame = 0


Query: 7   GSCLDEASLVSRLNNKLMPIALNNLAIQLKGFKYTIFDSYTMLLQRMENPSKYGFKEGIT 66
           GSC +  + +  L+N+  P  L  L  +L GFKY + D +T L QR+ NPS+YGFKEG  
Sbjct: 246 GSCFEPVTELINLHNQEFPKVLRRLERRLSGFKYALHDFHTSLSQRINNPSRYGFKEGEM 305

Query: 67  ACCGSERFRGVYSCGGKRLVEE-YELCENPNDYLFFDSFHLTEKGYEQFAKLMWSGDAKV 126
           ACCGS   RG+ +CG +    + Y+LCEN +DY+FFD  HLTE  ++Q A+L+WSG   V
Sbjct: 306 ACCGSGPLRGINTCGFRNGPSQGYKLCENADDYVFFDPSHLTETAHQQIAELIWSGPPNV 365

Query: 127 MKPYNLKQLF 136
             PYNLK LF
Sbjct: 366 TAPYNLKTLF 375

The following BLAST results are available for this feature:

BLAST of Lag0041665 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897092.1	1.2e-49	69.12	GDSL lipase-like [Benincasa hispida]	[more]
XP_008462253.1	1.3e-48	67.88	PREDICTED: GDSL esterase/lipase 5-like isoform X1 [Cucumis melo] >KAA0059365.1 G...	[more]
XP_022146472.1	2.3e-48	63.04	GDSL esterase/lipase 1-like [Momordica charantia]	[more]
XP_004141726.1	1.9e-47	66.18	GDSL lipase isoform X1 [Cucumis sativus] >KGN45451.1 hypothetical protein Csa_01...	[more]
XP_008462251.1	7.4e-47	64.96	PREDICTED: GDSL esterase/lipase 5-like [Cucumis melo] >KAA0059366.1 GDSL esteras...	[more]

BLAST of Lag0041665 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSA7	1.6e-36	51.13	GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2	[more]
Q9SYF0	9.7e-34	51.91	GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1	[more]
Q9FLN0	8.2e-33	51.54	GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1	[more]
Q9SYF5	2.0e-31	49.62	GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2	[more]
Q9LJP1	4.5e-31	49.23	GDSL esterase/lipase 4 OS=Arabidopsis thaliana OX=3702 GN=GLIP4 PE=2 SV=2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 2..123 e-value: 4.8E-22 score: 80.8
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 6..117 e-value: 2.3E-7 score: 31.5
None	No IPR available	PANTHER	PTHR45966:SF4	GDSL ESTERASE/LIPASE 5	coord: 2..136
IPR044552	GDSL esterase/lipase GLIP1-5/GLL25	PANTHER	PTHR45966	GDSL-LIKE LIPASE/ACYLHYDROLASE	coord: 2..136

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0041665.1	Lag0041665.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016298	lipase activity
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds