Lag0004805 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0004805
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNA pseudouridine synthase 3, mitochondrial
Locationchr6: 7207643 .. 7211141 (-)
RNA-Seq ExpressionLag0004805
SyntenyLag0004805
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGCAGAGCATTCGACGACATCTTCTACTTTCAACCAGATACTACTCTAGAATTTCTCCTCCATCACCTGCCTATGCTAAACCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGCAGTCCTAAAGAGGGCCCAAAGCCTCGGCAGCTCCTATCGCTGCCCCCTTTTCCTGGTTACCCTTTGCCTGGCAAGAATCAGATTGGTGTCTCTGGGGAGTCATCTCATGTCACTGCTATAAAATGGGTCAAGCATTACTTTGATGAAATTCACGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTGTAAGTATGAACACTCGCTCCTCTTTTAGCTGATTGGTTTTGTCCTTTTGTCTTTTTCCTGCTATGCTTATATCTAGTCTCGTGGGCTTCGTGTAACTTTTGTCATGTCAAGAATCCCCATTCAACTTTTTGAGCAGTGAGATGAATGCTAACTTGTAAGATGTAATTAGTAACCGGAGCTTAAACTCTTCTTGTTTCTTAAAGAAATTGAGTTCCATACTTATCTTGAGTTATTATTATAAATAAACTTACATTTCTGCTGTGGGAAAAAAGTACATTGGACTTGTTTTCTGAATAGAATTTCAGTTAGTGCCCGTGTCATATCTTTGAGGGAAAAACTCAAGGATATATCACAACTTCAATGTTGCTTATGGATCTGACCTCTCTCTTTCTCTCTCCAAAAAAAGGGGGAAAAAACGATGGATGGTTATCTAATCAATTTTGGGTTTAAATCTATCTTCCTTTTCCAGGTCCAGGTGGAATGTCCACGATCAGGAGGCTCAGATGGAGAAGGAAATTTGAAACATATGAGAAAGGTGTTGTTTCTTGAATAAGTAGCCACTCTTACGATCCGTTGTTTATGCATACATGATATCTTGTTTCGTTTACTTATTGAAGTCGTTCTTTTTCGTTGTTGGTCTATTAACTTATTACTAGATGACTTTCAGATTAGGAGTAATGAGATTATGGAAACTGGGGCAAGAGTTCATATACCTGTGTCAGTTGCAGAGATGAAGATTTCTAGGAGATTTGACACTATCCCAAGTGGCACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAAAGACTTGTCAAGTACAAGGCGAGTATTGAGTTTAACTTGCATTCTCCTCTTTGCTGCAGTTAATGAATCTCTCAGTGTTTTACTTACCCTTTGATATAACAAGCAGTTACTATATACTCGGTAATATCGGTATGGAAAGATCCACATAGATGTACTCATTTTCTCATGTACAATGTACCTTGGTTGAGTTTGGTGATTAATTAATTTCCTTCTCTCCATTGCTTCATTTTACCTTGGTATATGATTTCTGTTATATGCTAGACACGGTTTCATTTGATTGTTAATTTTGTAAATTTCAGGATTCTGCTATTATCGTGTTAAATAAACCCCCCAAACTACCTGTCAAGGTTTAGCTCCTATTCTCTTTCATTTTTCTATTTTATTTCTGGTGATGGGTGGGGCGGTCAGGTGGGAGGTTGCTCTGATATTTTGAAACAATTCTGGTATTGTCAGGGGAATCTTCCCATTCATAATAGTATGGATGCGTTGGCGGCTGCAGCATTATCTTATGATTATGATGATGGTCCAAAGTTGGTAAGCGTCTTGTTTACATTTAAGTAACTTGTGTAGATATTGTTATTAATAGACTATGTATTGAGAATGAAATAGCTAAGGAACTATGTTAATATGTATTTGTTGGATAATTGTGGGAATAAGATAGGTTGATACCATGGCCATGCGATTACATTGTGATTCAGCTGGGAATTTTGGTAATGTATGACAGAAGTATTTGCTTTAAGTTGTAACAATTATGAGGTATTTGTCTCCTAGACATGGGATAAATTTGGATGGACAAAGCTCCTTTGTAGTGGGCTTTTCTTCTTTTTGTATAGCCGGAAGCTTCGATTTTTGTTGAAAATGCTATCGATCCAACGTTGGCAAATATCATTCAACTGAAGAAGTTACATAGAAATTAAGTTTCCTGTTTTTTAATTTCAAGAAAATTGTGTTAATTCCTTAAAAATAAGGACGTGGAAGCTAGTATGTTCTCTTATTTTTCATCTGCTGTGGCATCTTTGGTTGCCTTGTTCTACATCTAGTGCTCTTCCTCTATCTATTCTTATGGATGCTCATTTAGGTTCACCGTTTGGATAGAGAGAGCAGCGGGATTCTCTTGATGGGGAGAACAAAAGAGAGTGTAGCCCACCTGCAATGGCTATTTAGCAATATAAGAAATGCGAAATCTTCATGTAAGGTGCTTAATGACAGTTACCTAGTTCCTGTGGCTAAGAAATGTTCTAGAATTTTTAATTGTTGAAAGTTCATAATATGCTCCACAATGATTTTAGGCTTGGGACGAGGCATGTAATGCAACATATCAGAAGTATTGGGCGTTAGTAATTGGTTGTCCCAGAGAAAGAGAAGGCTTAATTTCCGCTCCTCTTTCAAAGGTTCTCTCTCTCTCCTTCTCTCTCTGGGATCAATGTTCATGATTCTCAATCTTTGCAGGTGGTAATGTACTACGCAATAGTTAATAATTATAGATCAACATTATCATGTACAGCGGTACAATGGTTTGCTCCTCGGTTTACATGTAGTTACAGTTACTCTGCCTTTAGAAACTGTTCAATATATCTTCTAGAACTTGTAACTATGGGTCAAACAAGTTTCTTTTTAGGATTATCTTGTGTCACTCAACTGTGTTCTAATTGATTATAGGTGCTTCTTGATGATGGGAAGACCGAGAGGGTTGTCTTGGCGAACAGAACGTCCTTGGAAGCTTCTCAGGAGGCAATCACTGAATATCGTGTGCTTGGTCCCACAATTAATGGTTGTTCGTGGATTGAACTATGCCCACGTACTAGCCGAAAGCATCAGGTACTTCAAGCTCTCTCTCTCCTATGCACAAGCTCATGCAAATCTATGCATGTAAATCTTGACTATATCATCTTTAGTACTATTCCTGTGAACCATGAAGGAAAGTATCTGCATTCGAGTGATTCTTTATGGAGTTAAACACTCCTTCCTATTCTAAAGTAACTTCATTTTGTACACAGCTACGGATCCATTGTGCTGAAGCTCTGGGCACGCCTATTGTTGGTGACTACAAATACGGTTGGTTTGTACATCGGACGTGGAAACAAATGCCTCGAGTCGACATTGAGCCGATAAGTGGGAAGCCGTATAAGTTGCGGAGGCCAGAAGGGCTGGATGTGCAGAAGGGGAGTGTTTTATCCAAGGTGCCTTTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTCGTTCGTGTTTTGGATGATAAAAGGTCGGTCAGCCAGTACACGGTATCTAATACCTTGAACTCAGATCTCTTACGTTTTGTAGCAAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTGTAA

mRNA sequence

ATGCGGCAGAGCATTCGACGACATCTTCTACTTTCAACCAGATACTACTCTAGAATTTCTCCTCCATCACCTGCCTATGCTAAACCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGCAGTCCTAAAGAGGGCCCAAAGCCTCGGCAGCTCCTATCGCTGCCCCCTTTTCCTGGTTACCCTTTGCCTGGCAAGAATCAGATTGGTGTCTCTGGGGAGTCATCTCATGTCACTGCTATAAAATGGGTCAAGCATTACTTTGATGAAATTCACGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTGTCCAGGTGGAATGTCCACGATCAGGAGGCTCAGATGGAGAAGGAAATTTGAAACATATGAGAAAGATTAGGAGTAATGAGATTATGGAAACTGGGGCAAGAGTTCATATACCTGTGTCAGTTGCAGAGATGAAGATTTCTAGGAGATTTGACACTATCCCAAGTGGCACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAAAGACTTGTCAAGTACAAGGATTCTGCTATTATCGTGTTAAATAAACCCCCCAAACTACCTGTCAAGGGGAATCTTCCCATTCATAATAGTATGGATGCGTTGGCGGCTGCAGCATTATCTTATGATTATGATGATGGTCCAAAGTTGGTTCACCGTTTGGATAGAGAGAGCAGCGGGATTCTCTTGATGGGGAGAACAAAAGAGAGTGTAGCCCACCTGCAATGGCTATTTAGCAATATAAGAAATGCGAAATCTTCATGTAAGGCTTGGGACGAGGCATGTAATGCAACATATCAGAAGTATTGGGCGTTAGTAATTGGTTGTCCCAGAGAAAGAGAAGGCTTAATTTCCGCTCCTCTTTCAAAGGTGCTTCTTGATGATGGGAAGACCGAGAGGGTTGTCTTGGCGAACAGAACGTCCTTGGAAGCTTCTCAGGAGGCAATCACTGAATATCGTGTGCTTGGTCCCACAATTAATGGTTGTTCGTGGATTGAACTATGCCCACGTACTAGCCGAAAGCATCAGCTACGGATCCATTGTGCTGAAGCTCTGGGCACGCCTATTGTTGGTGACTACAAATACGGTTGGTTTGTACATCGGACGTGGAAACAAATGCCTCGAGTCGACATTGAGCCGATAAGTGGGAAGCCGTATAAGTTGCGGAGGCCAGAAGGGCTGGATGTGCAGAAGGGGAGTGTTTTATCCAAGGTGCCTTTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTCGTTCGTGTTTTGGATGATAAAAGGTCGGTCAGCCAGTACACGGTATCTAATACCTTGAACTCAGATCTCTTACGTTTTGTAGCAAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTGTAA

Coding sequence (CDS)

ATGCGGCAGAGCATTCGACGACATCTTCTACTTTCAACCAGATACTACTCTAGAATTTCTCCTCCATCACCTGCCTATGCTAAACCTGTCATTAGAGTTTCCAACAACATAGCACAGTTGGGCAGTCCTAAAGAGGGCCCAAAGCCTCGGCAGCTCCTATCGCTGCCCCCTTTTCCTGGTTACCCTTTGCCTGGCAAGAATCAGATTGGTGTCTCTGGGGAGTCATCTCATGTCACTGCTATAAAATGGGTCAAGCATTACTTTGATGAAATTCACGATTCTGTAATTCAGTCCCATTTCAGAAAAGGTCTTGTCCAGGTGGAATGTCCACGATCAGGAGGCTCAGATGGAGAAGGAAATTTGAAACATATGAGAAAGATTAGGAGTAATGAGATTATGGAAACTGGGGCAAGAGTTCATATACCTGTGTCAGTTGCAGAGATGAAGATTTCTAGGAGATTTGACACTATCCCAAGTGGCACATTGTACCCAAACGCTGATGAAATCAAGTACTTGCAAAGACTTGTCAAGTACAAGGATTCTGCTATTATCGTGTTAAATAAACCCCCCAAACTACCTGTCAAGGGGAATCTTCCCATTCATAATAGTATGGATGCGTTGGCGGCTGCAGCATTATCTTATGATTATGATGATGGTCCAAAGTTGGTTCACCGTTTGGATAGAGAGAGCAGCGGGATTCTCTTGATGGGGAGAACAAAAGAGAGTGTAGCCCACCTGCAATGGCTATTTAGCAATATAAGAAATGCGAAATCTTCATGTAAGGCTTGGGACGAGGCATGTAATGCAACATATCAGAAGTATTGGGCGTTAGTAATTGGTTGTCCCAGAGAAAGAGAAGGCTTAATTTCCGCTCCTCTTTCAAAGGTGCTTCTTGATGATGGGAAGACCGAGAGGGTTGTCTTGGCGAACAGAACGTCCTTGGAAGCTTCTCAGGAGGCAATCACTGAATATCGTGTGCTTGGTCCCACAATTAATGGTTGTTCGTGGATTGAACTATGCCCACGTACTAGCCGAAAGCATCAGCTACGGATCCATTGTGCTGAAGCTCTGGGCACGCCTATTGTTGGTGACTACAAATACGGTTGGTTTGTACATCGGACGTGGAAACAAATGCCTCGAGTCGACATTGAGCCGATAAGTGGGAAGCCGTATAAGTTGCGGAGGCCAGAAGGGCTGGATGTGCAGAAGGGGAGTGTTTTATCCAAGGTGCCTTTGTTACATCTTCACTGCAGAGAGCTTGTTCTTCCCAATATTGCAAAGTTCGTTCGTGTTTTGGATGATAAAAGGTCGGTCAGCCAGTACACGGTATCTAATACCTTGAACTCAGATCTCTTACGTTTTGTAGCAAGAATGCCTTCCCATATGAGAATTAGCTGGAATTTGATGTCTTCCTATTTAGTGTAA

Protein sequence

MRQSIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGNLKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKDSAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV
Homology
BLAST of Lag0004805 vs. NCBI nr
Match: XP_022997125.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima] >XP_022997126.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima])

HSP 1 Score: 916.4 bits (2367), Expect = 1.0e-262
Identity = 442/474 (93.25%), Postives = 461/474 (97.26%), Query Frame = 0

Query: 1   MRQSIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MRQSIRRHLLL+ RYYSRISPP PAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLAARYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGN 120
           YPLP KNQIG SGES+HVTAI WVKHYFDEIHDSVIQSHFRKGLVQV+CP+SGG DGEGN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LK MRKIRSNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 SAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAI+VLNKPP+LPVKGNLP+HNSMDALAAAALSYDYDDGPKLVHRLDRESSGI+LMGRTK
Sbjct: 181 SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRTK 240

Query: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300
           ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 301 GKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERVVLAN+TS EASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKTSFEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCREL 420

Query: 421 VLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           VLPN+AKF+ VLDDKRS+S + VSN LNSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 VLPNLAKFLNVLDDKRSISHHRVSN-LNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Lag0004805 vs. NCBI nr
Match: XP_022961737.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata] >XP_022961738.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata])

HSP 1 Score: 912.1 bits (2356), Expect = 1.9e-261
Identity = 441/474 (93.04%), Postives = 460/474 (97.05%), Query Frame = 0

Query: 1   MRQSIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MRQSIRRHLLL+TRYYSRISPP PAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGN 120
           YPLP KNQIG SGES+HVTAI WVKHYFDEI DSVIQSHFRKGLVQV+CP+SGG DGEGN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIEDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LK MRKIRSNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 SAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAI+VLNKPP+LPVKGNLP+HNSMDALAAAALSYDYDDGPKLVHRLDRESSGI+LMGRTK
Sbjct: 181 SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRTK 240

Query: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300
           ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 301 GKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERVVLAN+TSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCREL 420

Query: 421 VLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           VLPN+AKF+ VLDDKRS+S + VS   NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 VLPNLAKFLNVLDDKRSISHHRVS-YFNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Lag0004805 vs. NCBI nr
Match: XP_022131827.1 (RNA pseudouridine synthase 3, mitochondrial isoform X3 [Momordica charantia])

HSP 1 Score: 911.8 bits (2355), Expect = 2.5e-261
Identity = 442/474 (93.25%), Postives = 462/474 (97.47%), Query Frame = 0

Query: 1   MRQSIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLL+ RYYSRISPP PAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGN 120
           YPLP KN IG+SG S+HVTAI WVKHYFDEIHDSVIQSHFRKGL+QVECP+SGGS+G+GN
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120

Query: 121 LKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LK MRKI+SNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 181 SAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAIIVLNKPPKLPVKGNLP+HNSMDALAAAALSYDYD+GPKLVHRLDRESSGILLMGRTK
Sbjct: 181 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTK 240

Query: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300
           ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 301 GKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERVVLA++TSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420

Query: 421 VLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           VLPNIAKF+ VL +KRS+SQ+ VS++ NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 VLPNIAKFIDVLVNKRSISQHPVSSS-NSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Lag0004805 vs. NCBI nr
Match: XP_023545207.1 (RNA pseudouridine synthase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023545209.1 RNA pseudouridine synthase 3, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 906.0 bits (2340), Expect = 1.4e-259
Identity = 439/474 (92.62%), Postives = 458/474 (96.62%), Query Frame = 0

Query: 1   MRQSIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M QSIRRHLLL+TRYYSRISPP PAYA+PVIRVSNN+AQLGS KEG KPRQLLSLPPFPG
Sbjct: 1   MWQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSRKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGN 120
           YPLP KNQIG SGES+HVTAI WVKHYFDEIHDSVIQSHFRKGLVQV+CP+SGG DGEGN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LK MRKIRSNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 SAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAI+VLNKPP+LPVKGNLP+HNSMDALAAAALSYDYDDGPKLVHRLDRESSGI+LMGRTK
Sbjct: 181 SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRTK 240

Query: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300
           ESVAHLQWLFSNIRNAKSSCKAW EACNATYQKYWALVIGCPREREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWGEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 301 GKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERVVLAN+TSLEA QEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKTSLEAFQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCREL 420

Query: 421 VLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           VLPN+AKF+ VLDD RS+S + VSN LNSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 VLPNLAKFLNVLDDTRSISHHRVSN-LNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Lag0004805 vs. NCBI nr
Match: XP_038885249.1 (LOW QUALITY PROTEIN: RNA pseudouridine synthase 3, mitochondrial [Benincasa hispida])

HSP 1 Score: 894.8 bits (2311), Expect = 3.2e-256
Identity = 432/468 (92.31%), Postives = 452/468 (96.58%), Query Frame = 0

Query: 7   RHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPGK 66
           RHLLL+TRYYSRISPP P+YAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG+PLPGK
Sbjct: 50  RHLLLATRYYSRISPPPPSYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGHPLPGK 109

Query: 67  NQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGNLKHMRK 126
           NQIGVSGES+HVTAI+WVKHYFDEI DSVIQSHFRKGLVQVEC + GG D +GN K MRK
Sbjct: 110 NQIGVSGESTHVTAIRWVKHYFDEIEDSVIQSHFRKGLVQVECHKXGGLDVKGNSKPMRK 169

Query: 127 IRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKDSAIIVL 186
           IRS+EI+ETGAR+HIPVSVAE+K+SRRFDTIPSGTLYPNADEIKYLQRLVKYKDSAIIVL
Sbjct: 170 IRSSEIVETGARIHIPVSVAELKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKDSAIIVL 229

Query: 187 NKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHL 246
           NKPPKLPVKGNLP+HNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHL
Sbjct: 230 NKPPKLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHL 289

Query: 247 QWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERV 306
           QWLFSNIRNA+S+CKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERV
Sbjct: 290 QWLFSNIRNARSACKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERV 349

Query: 307 VLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYK 366
           VLAN+TSLEASQEAITEYRVLGPTINGCSWIELCP TSRKHQLRIHCAEALGTPIVGDYK
Sbjct: 350 VLANKTSLEASQEAITEYRVLGPTINGCSWIELCPHTSRKHQLRIHCAEALGTPIVGDYK 409

Query: 367 YGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIA 426
           YGWFVHRTWKQMPRVD+EPISGKPYKLRRP GLDVQKGSVLSKVPLLHLHCRELVLPN A
Sbjct: 410 YGWFVHRTWKQMPRVDVEPISGKPYKLRRPAGLDVQKGSVLSKVPLLHLHCRELVLPNFA 469

Query: 427 KFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           KFV VLDDKR +S + VS +LNSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 470 KFVHVLDDKRPISHHPVS-SLNSDLLRFVARMPSHMRISWNLMSSYLV 516

BLAST of Lag0004805 vs. ExPASy Swiss-Prot
Match: Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 1.4e-193
Identity = 329/473 (69.56%), Postives = 392/473 (82.88%), Query Frame = 0

Query: 8   HLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPGKN 67
           +L +  R YSR++PP  +    VIRV+NN+A LG PK+GP PRQL+SLPPFPG+PLPGKN
Sbjct: 12  YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71

Query: 68  QIGVSGE----SSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVEC--PRSGGSDGEGNL 127
             G  G+      HVTAI WVK+YF+EI+D  IQ+HF KGLVQ+E    R    + E   
Sbjct: 72  -AGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDGA 131

Query: 128 KHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKDS 187
             MRKI+ NE+M+ G ++ +PVS+AEM+IS+R+DTIPSGTLYPNADEI YLQRLV++KDS
Sbjct: 132 IPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKDS 191

Query: 188 AIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKE 247
           AIIVLNKPPKLPVKGN+PIHNSMDALAAAALS+  D+GP+LVHRLDRE+SG+L+MGRTKE
Sbjct: 192 AIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRTKE 251

Query: 248 SVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDG 307
           S+ +L  +FS+ +   SSCKAW++AC A YQ+YWALVIG P+E+EGLISAPLSKVLLDDG
Sbjct: 252 SIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLDDG 311

Query: 308 KTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPI 367
           KT+RVVLA  +  EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGTPI
Sbjct: 312 KTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGTPI 371

Query: 368 VGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELV 427
           VGDYKYGWFVH+ WKQMP+VDIEP +GKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE+V
Sbjct: 372 VGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREMV 431

Query: 428 LPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           LPNIAKF+ V++ + +   +T       DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 LPNIAKFLHVMNQQETEPLHT-GIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

BLAST of Lag0004805 vs. ExPASy Swiss-Prot
Match: Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 3.9e-172
Identity = 295/482 (61.20%), Postives = 373/482 (77.39%), Query Frame = 0

Query: 6   RRHLLLSTRYYSRISPPSPAYAKP-VIRV-SNNIAQLGSPKEGPKPRQLLSLPPFP---- 65
           RR +  + R+ SR++PP+PA A P V+RV  +N+A+LG PK GP+PRQLLSLPPFP    
Sbjct: 5   RRRVGAAVRWLSRLAPPAPAEADPVVVRVDGSNVARLGKPKPGPRPRQLLSLPPFPGGGD 64

Query: 66  GYPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEG 125
           G PLPG+     +     VTA+ WVKHY  ++   V+Q+HF K LV  EC     SD E 
Sbjct: 65  GDPLPGRK----AAAPRRVTAVSWVKHYLADVPQEVVQAHFNKRLVYSEC-----SDHEV 124

Query: 126 NLK-------HMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYL 185
           +++       H++KI+ N++ME G R+H+PVSVAE +I +R++TIP+ TL+PN DEI+YL
Sbjct: 125 SVETIKSQKHHLKKIKHNDVMEPGMRIHLPVSVAEGEIKKRYETIPTATLHPNKDEIEYL 184

Query: 186 QRLVKYKDSAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSG 245
           +RLV +KDSAI+VLNKPPK+P+KGNLP+HNSMD LAAAALSY  ++GPKLVHRLDRESSG
Sbjct: 185 RRLVIHKDSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKLVHRLDRESSG 244

Query: 246 ILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAP 305
           +LL GRTKES   L WLF+++  AK++ + W+ AC A  Q+YWALVIG P+EREG+ISAP
Sbjct: 245 LLLFGRTKESFTRLHWLFTSVNLAKTNSQVWNAACEAYMQRYWALVIGTPKEREGIISAP 304

Query: 306 LSKVLLDDGKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIH 365
           LSKVLLDDGK ERV+LA+ + ++ +QEA+T YRV+GPTI+GCSWIEL P T RKHQLR+H
Sbjct: 305 LSKVLLDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRPLTGRKHQLRVH 364

Query: 366 CAEALGTPIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPL 425
           CAEALGTPIVGDYKYGWFVH+ WKQ P+ D EP +G+PYKLRRPEGL++QKGSVLSKVPL
Sbjct: 365 CAEALGTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLEIQKGSVLSKVPL 424

Query: 426 LHLHCRELVLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSY 475
           LHLHCRE+V+PNIAKF+    +             N  LLRF+A MP+HM+ISWN+MSSY
Sbjct: 425 LHLHCREMVIPNIAKFLSSNGEWHENGAPWSKEKPN--LLRFIAPMPAHMKISWNIMSSY 475

BLAST of Lag0004805 vs. ExPASy Swiss-Prot
Match: Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 4.9e-74
Identity = 171/477 (35.85%), Postives = 266/477 (55.77%), Query Frame = 0

Query: 4   SIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGY-- 63
           ++RR L  S+   S    P+ A +    + + + +   +     K    L+LPPF     
Sbjct: 10  TLRRQLQSSSPTISTFKNPTKALSAAAHQSTRSYSTTQTDDSRGK---WLTLPPFSPTID 69

Query: 64  -------PLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGG 123
                   L   + +  S ++S  TA++W+     ++  +++Q  FR   V+ E   S  
Sbjct: 70  GTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFRLRQVRREMSMSVD 129

Query: 124 SDGEGNLKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQR 183
            D E     ++++ + E +  G R+++P+SV     + +       +   + +E K++  
Sbjct: 130 GD-ELQRSQLKRVAAKESLNVGDRIYLPLSVD--NDTPQTPPAKKESFQCSDEERKFVCS 189

Query: 184 LVKYKDSAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIL 243
           LV YKD AIIVLNKP  L V+G   I  S+D LAA+ L +D  + P+LVHRLDR+ SG+L
Sbjct: 190 LVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLL 249

Query: 244 LMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLS 303
           ++ RT+ +   L  +F   +   +S     +   +  +KY ALVIGCP  + G ISAPL 
Sbjct: 250 VLARTQTAATVLHSIFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPPRQRGQISAPLR 309

Query: 304 KVLLDDGKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCA 363
           KV++DDGK+ER+ + +   L ++Q AITEYRV+  + +G +W+EL P T RKHQLR+HCA
Sbjct: 310 KVVVDDGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCA 369

Query: 364 EALGTPIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLH 423
           E LGTPIVGDYKYGW  H+  +     +  P      +   P GLD+  G V SK P LH
Sbjct: 370 EVLGTPIVGDYKYGWQAHKAREPFVSSENNPTK----QSSSPFGLDLDGGDVSSKQPHLH 429

Query: 424 LHCRELVLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSS 472
           LH +++ LPNI++ +    +K  VS  + S+  + D L+F A +PSHM++S+NL+ S
Sbjct: 430 LHSKQIDLPNISQLL----EKMQVS--SDSDISDLDSLKFDAPLPSHMQLSFNLLKS 469

BLAST of Lag0004805 vs. ExPASy Swiss-Prot
Match: Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 4.3e-46
Identity = 106/281 (37.72%), Postives = 164/281 (58.36%), Query Frame = 0

Query: 70  GVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQ--VECPRSGGSDGEGNLKHMRKI 129
           G  GE    TAIKWV+    ++  S++Q  FR   V+  V       +D       +R++
Sbjct: 77  GGDGEQG-ATAIKWVRRCCPDLPTSLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRV 136

Query: 130 RSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKDSAIIVLN 189
            + + +  G  + +PV++ E  ++ +         + N +EI +L+ L  YKD AIIV+N
Sbjct: 137 SAKDQLMPGDILFLPVNLKESSVAEKTKK------FDNRNEINFLRGLEIYKDEAIIVVN 196

Query: 190 KPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVAHLQ 249
           KPP +PV+G + I NS+D L A+    +  + P+LVHRLDR+ SGIL++GR + S + L 
Sbjct: 197 KPPGMPVQGGVGIKNSIDVL-ASMFEENSSEAPRLVHRLDRDCSGILVLGRNQLSTSMLH 256

Query: 250 WLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDGKTERVV 309
            +F      K++    D   +   +KY ALVIG PR  +GL+SAPL+K+LL DGK+ER+ 
Sbjct: 257 AIF----REKTADALADGTQHVLQRKYVALVIGTPRHPKGLLSAPLAKILLQDGKSERLT 316

Query: 310 LANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQ 349
           +   ++  + Q+A+TEYRV+     G +W+EL PRT RKHQ
Sbjct: 317 IRASSNAASVQDALTEYRVIESCPQGYTWLELFPRTGRKHQ 345

BLAST of Lag0004805 vs. ExPASy Swiss-Prot
Match: Q28C59 (Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN=rpusd4 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.7e-26
Identity = 89/284 (31.34%), Postives = 132/284 (46.48%), Query Frame = 0

Query: 165 NADEIKYLQRL---VKYKDSAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYD---D 224
           NA  I YL+ L   V  +D  ++++NKP  LPV G   +  S+ +L  A   + +    +
Sbjct: 37  NAPTIVYLRELKSQVVREDPELVLVNKPHGLPVHGGPTVERSVASLLPALAKHHFGWKAE 96

Query: 225 GPKLVHRLDRESSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALV 284
             KL HRLDR+++G L++ RT E+   +Q                       ++ YWAL 
Sbjct: 97  PLKLCHRLDRDTTGALILARTTEAAERVQQALRE---------------REVHRVYWALC 156

Query: 285 IGCPREREGLISAPLSKV------------------LLDDGKTERVVLANRTSLEASQEA 344
           +G P  REG++  P+ +                   + ++G  ERV +       ++ EA
Sbjct: 157 LGTPSPREGILDIPIMEKETSGPQKHYKMALSPRFRVSEEGAVERVRVP-----RSAHEA 216

Query: 345 ITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYKYGWFVHRTWKQMPR 404
           +T YR LG   +G S +EL P T  KHQLR+H A  L  PI+GD+KY  +  R   Q P 
Sbjct: 217 VTRYRTLG-AASGASLVELHPITGVKHQLRVHLALGLNCPILGDHKYSHW-GRLAPQKP- 276

Query: 405 VDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPN 425
                    P  + R  GL V +   LS    LHLH  +L LP+
Sbjct: 277 ---------PDSVLRALGLTVPQARTLS----LHLHAVQLTLPS 284

BLAST of Lag0004805 vs. ExPASy TrEMBL
Match: A0A6J1K8P8 (RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111492130 PE=4 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 5.0e-263
Identity = 442/474 (93.25%), Postives = 461/474 (97.26%), Query Frame = 0

Query: 1   MRQSIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MRQSIRRHLLL+ RYYSRISPP PAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLAARYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGN 120
           YPLP KNQIG SGES+HVTAI WVKHYFDEIHDSVIQSHFRKGLVQV+CP+SGG DGEGN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIHDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LK MRKIRSNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 SAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAI+VLNKPP+LPVKGNLP+HNSMDALAAAALSYDYDDGPKLVHRLDRESSGI+LMGRTK
Sbjct: 181 SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRTK 240

Query: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300
           ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 301 GKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERVVLAN+TS EASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKTSFEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCREL 420

Query: 421 VLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           VLPN+AKF+ VLDDKRS+S + VSN LNSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 VLPNLAKFLNVLDDKRSISHHRVSN-LNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Lag0004805 vs. ExPASy TrEMBL
Match: A0A6J1HAX8 (RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111462419 PE=4 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 9.4e-262
Identity = 441/474 (93.04%), Postives = 460/474 (97.05%), Query Frame = 0

Query: 1   MRQSIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           MRQSIRRHLLL+TRYYSRISPP PAYA+PVIRVSNN+AQLGSPKEG KPRQLLSLPPFPG
Sbjct: 1   MRQSIRRHLLLATRYYSRISPPPPAYARPVIRVSNNVAQLGSPKEGSKPRQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGN 120
           YPLP KNQIG SGES+HVTAI WVKHYFDEI DSVIQSHFRKGLVQV+CP+SGG DGEGN
Sbjct: 61  YPLPRKNQIGFSGESTHVTAITWVKHYFDEIEDSVIQSHFRKGLVQVQCPQSGGLDGEGN 120

Query: 121 LKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LK MRKIRSNEIMETGARVHIPVSVAEM++SRRFDTIPSGTL+PNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSNEIMETGARVHIPVSVAEMRVSRRFDTIPSGTLHPNADEIKYLQRLVKYKD 180

Query: 181 SAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAI+VLNKPP+LPVKGNLP+HNSMDALAAAALSYDYDDGPKLVHRLDRESSGI+LMGRTK
Sbjct: 181 SAILVLNKPPRLPVKGNLPVHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIILMGRTK 240

Query: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300
           ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 301 GKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERVVLAN+TSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRP+GLDVQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPKGLDVQKGSVLSKVPLLHLHCREL 420

Query: 421 VLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           VLPN+AKF+ VLDDKRS+S + VS   NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 VLPNLAKFLNVLDDKRSISHHRVS-YFNSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Lag0004805 vs. ExPASy TrEMBL
Match: A0A6J1BS40 (RNA pseudouridine synthase 3, mitochondrial isoform X3 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.2e-261
Identity = 442/474 (93.25%), Postives = 462/474 (97.47%), Query Frame = 0

Query: 1   MRQSIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLL+ RYYSRISPP PAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGN 120
           YPLP KN IG+SG S+HVTAI WVKHYFDEIHDSVIQSHFRKGL+QVECP+SGGS+G+GN
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLIQVECPQSGGSNGKGN 120

Query: 121 LKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LK MRKI+SNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 181 SAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAIIVLNKPPKLPVKGNLP+HNSMDALAAAALSYDYD+GPKLVHRLDRESSGILLMGRTK
Sbjct: 181 SAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRESSGILLMGRTK 240

Query: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300
           ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD
Sbjct: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300

Query: 301 GKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERVVLA++TSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420

Query: 421 VLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           VLPNIAKF+ VL +KRS+SQ+ VS++ NSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 VLPNIAKFIDVLVNKRSISQHPVSSS-NSDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Lag0004805 vs. ExPASy TrEMBL
Match: A0A6J1BQS0 (RNA pseudouridine synthase 3, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004890 PE=4 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 5.3e-249
Identity = 432/484 (89.26%), Postives = 449/484 (92.77%), Query Frame = 0

Query: 1   MRQSIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M+QSIRRHLLL+ RYYSRISPP PAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG
Sbjct: 1   MQQSIRRHLLLAARYYSRISPPPPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60

Query: 61  YPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGN 120
           YPLP KN IG+SG S+HVTAI WVKHYFDEIHDSVIQSHFRKGLV       GG    G 
Sbjct: 61  YPLPRKNHIGISGGSTHVTAINWVKHYFDEIHDSVIQSHFRKGLV-----NPGGMSTIGW 120

Query: 121 LKHMRK----------IRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180
           +K  RK          I+SNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK
Sbjct: 121 IKWKRKFETYEKDDFQIKSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIK 180

Query: 181 YLQRLVKYKDSAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRES 240
           YLQRLVKYKDSAIIVLNKPPKLPVKGNLP+HNSMDALAAAALSYDYD+GPKLVHRLDRES
Sbjct: 181 YLQRLVKYKDSAIIVLNKPPKLPVKGNLPVHNSMDALAAAALSYDYDEGPKLVHRLDRES 240

Query: 241 SGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLIS 300
           SGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLIS
Sbjct: 241 SGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLIS 300

Query: 301 APLSKVLLDDGKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLR 360
           APLSKVLLDDGKTERVVLA++TSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLR
Sbjct: 301 APLSKVLLDDGKTERVVLASKTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLR 360

Query: 361 IHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKV 420
           IHCAEALGTPIVGDYKYGWFVHRTWKQMPRVD+E ISGKPYKLRRPEGLDVQKGSVLSKV
Sbjct: 361 IHCAEALGTPIVGDYKYGWFVHRTWKQMPRVDVEQISGKPYKLRRPEGLDVQKGSVLSKV 420

Query: 421 PLLHLHCRELVLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMS 475
           PLLHLHCRELVLPNIAKF+ VL +KRS+SQ+ VS++ NSDLLRFVARMPSHMRISWNLMS
Sbjct: 421 PLLHLHCRELVLPNIAKFIDVLVNKRSISQHPVSSS-NSDLLRFVARMPSHMRISWNLMS 478

BLAST of Lag0004805 vs. ExPASy TrEMBL
Match: A0A0A0LQ04 (PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G402020 PE=4 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 1.7e-247
Identity = 420/477 (88.05%), Postives = 446/477 (93.50%), Query Frame = 0

Query: 1   MRQSIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPG 60
           M++S+RRHLLL+TR YSRISPP PAYAKP+IRVSNNIAQLGSPKEGPK RQLLSLPPFPG
Sbjct: 73  MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 132

Query: 61  YPLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGN 120
           YPLPGKNQ+GVSGES+HVTAI+WVK+YFDEI DS IQSHFRKGLVQVECP+ GG D +GN
Sbjct: 133 YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 192

Query: 121 LKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LK MRKIRS EIMETGAR+H+PVSVAEMK++RRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 193 LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD 252

Query: 181 SAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAIIVLNKPPKLPVKG LP+HNSMDALA AALSYDYDDGPKLVHRLDRESSGILLMGRTK
Sbjct: 253 SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK 312

Query: 241 ESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDD 300
           ESV HLQWLFSNIRNAK +CKAW+EACNATYQKYWALVIGCPREREG+ISAPLSKVLLDD
Sbjct: 313 ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD 372

Query: 301 GKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERVVLAN+ S+EASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 373 GKTERVVLANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 432

Query: 361 IVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHR WKQMPRVD+EPISG+PYKLRRPEGLDVQKGSVLSKVPLLHLHCREL
Sbjct: 433 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 492

Query: 421 VLPNIAKFVRVLDDK---RSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           VLPNIAKF+ V   K   R VS +      +SDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 493 VLPNIAKFLNVFYGKGPNRPVSSF------DSDLLRFVARMPSHMRISWNLMSSYLV 543

BLAST of Lag0004805 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 677.2 bits (1746), Expect = 9.8e-195
Identity = 329/473 (69.56%), Postives = 392/473 (82.88%), Query Frame = 0

Query: 8   HLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGYPLPGKN 67
           +L +  R YSR++PP  +    VIRV+NN+A LG PK+GP PRQL+SLPPFPG+PLPGKN
Sbjct: 12  YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71

Query: 68  QIGVSGE----SSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVEC--PRSGGSDGEGNL 127
             G  G+      HVTAI WVK+YF+EI+D  IQ+HF KGLVQ+E    R    + E   
Sbjct: 72  -AGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDGA 131

Query: 128 KHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKDS 187
             MRKI+ NE+M+ G ++ +PVS+AEM+IS+R+DTIPSGTLYPNADEI YLQRLV++KDS
Sbjct: 132 IPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKDS 191

Query: 188 AIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGILLMGRTKE 247
           AIIVLNKPPKLPVKGN+PIHNSMDALAAAALS+  D+GP+LVHRLDRE+SG+L+MGRTKE
Sbjct: 192 AIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRTKE 251

Query: 248 SVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLSKVLLDDG 307
           S+ +L  +FS+ +   SSCKAW++AC A YQ+YWALVIG P+E+EGLISAPLSKVLLDDG
Sbjct: 252 SIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLDDG 311

Query: 308 KTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPI 367
           KT+RVVLA  +  EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGTPI
Sbjct: 312 KTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGTPI 371

Query: 368 VGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELV 427
           VGDYKYGWFVH+ WKQMP+VDIEP +GKPYKLRRPEGLDVQKGSVLSKVPLLHLHCRE+V
Sbjct: 372 VGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREMV 431

Query: 428 LPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSSYLV 475
           LPNIAKF+ V++ + +   +T       DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 LPNIAKFLHVMNQQETEPLHT-GIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

BLAST of Lag0004805 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 280.0 bits (715), Expect = 3.5e-75
Identity = 171/477 (35.85%), Postives = 266/477 (55.77%), Query Frame = 0

Query: 4   SIRRHLLLSTRYYSRISPPSPAYAKPVIRVSNNIAQLGSPKEGPKPRQLLSLPPFPGY-- 63
           ++RR L  S+   S    P+ A +    + + + +   +     K    L+LPPF     
Sbjct: 10  TLRRQLQSSSPTISTFKNPTKALSAAAHQSTRSYSTTQTDDSRGK---WLTLPPFSPTID 69

Query: 64  -------PLPGKNQIGVSGESSHVTAIKWVKHYFDEIHDSVIQSHFRKGLVQVECPRSGG 123
                   L   + +  S ++S  TA++W+     ++  +++Q  FR   V+ E   S  
Sbjct: 70  GTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFRLRQVRREMSMSVD 129

Query: 124 SDGEGNLKHMRKIRSNEIMETGARVHIPVSVAEMKISRRFDTIPSGTLYPNADEIKYLQR 183
            D E     ++++ + E +  G R+++P+SV     + +       +   + +E K++  
Sbjct: 130 GD-ELQRSQLKRVAAKESLNVGDRIYLPLSVD--NDTPQTPPAKKESFQCSDEERKFVCS 189

Query: 184 LVKYKDSAIIVLNKPPKLPVKGNLPIHNSMDALAAAALSYDYDDGPKLVHRLDRESSGIL 243
           LV YKD AIIVLNKP  L V+G   I  S+D LAA+ L +D  + P+LVHRLDR+ SG+L
Sbjct: 190 LVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLL 249

Query: 244 LMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLISAPLS 303
           ++ RT+ +   L  +F   +   +S     +   +  +KY ALVIGCP  + G ISAPL 
Sbjct: 250 VLARTQTAATVLHSIFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPPRQRGQISAPLR 309

Query: 304 KVLLDDGKTERVVLANRTSLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCA 363
           KV++DDGK+ER+ + +   L ++Q AITEYRV+  + +G +W+EL P T RKHQLR+HCA
Sbjct: 310 KVVVDDGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKHQLRVHCA 369

Query: 364 EALGTPIVGDYKYGWFVHRTWKQMPRVDIEPISGKPYKLRRPEGLDVQKGSVLSKVPLLH 423
           E LGTPIVGDYKYGW  H+  +     +  P      +   P GLD+  G V SK P LH
Sbjct: 370 EVLGTPIVGDYKYGWQAHKAREPFVSSENNPTK----QSSSPFGLDLDGGDVSSKQPHLH 429

Query: 424 LHCRELVLPNIAKFVRVLDDKRSVSQYTVSNTLNSDLLRFVARMPSHMRISWNLMSS 472
           LH +++ LPNI++ +    +K  VS  + S+  + D L+F A +PSHM++S+NL+ S
Sbjct: 430 LHSKQIDLPNISQLL----EKMQVS--SDSDISDLDSLKFDAPLPSHMQLSFNLLKS 469

BLAST of Lag0004805 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 63.2 bits (152), Expect = 6.7e-10
Identity = 69/320 (21.56%), Postives = 122/320 (38.12%), Query Frame = 0

Query: 83  WVKHYFDEIHDSVIQSHFRKGLVQVECPRSGGSDGEGNLKHMRKIRSNEIMETGARVHIP 142
           W+    + +  + +QS  R GLV V            N + + K+  N  +++G  V+  
Sbjct: 87  WISSRINGVSRARVQSSIRLGLVTV------------NGRVVDKVSHN--VKSGDEVNCT 146

Query: 143 VSVAEMKISRRFDTIPSGTLYPNADEIKYLQRLVKYKDSAIIVLNKPPKL---PVKGN-- 202
           +S                 L P   E + +   + Y+D  ++V+NKP  +   P  GN  
Sbjct: 147 IS----------------ELQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPT 206

Query: 203 ------------LP----------------IHNSMDALAAAALSYDYDDGPKLVHRLDRE 262
                       LP                  +  + +  +  SY     P +VHRLD+ 
Sbjct: 207 GTLVNGILHHCSLPCVDYSNSEEDDDSDEETFSDDEEMTTSPSSYAASVRPGIVHRLDKG 266

Query: 263 SSGILLMGRTKESVAHLQWLFSNIRNAKSSCKAWDEACNATYQKYWALVIGCPREREGLI 322
           ++G+L++ + + S AHL   F                 +   + Y +L  G P   +G I
Sbjct: 267 TTGLLVVAKDEHSHAHLAEQFK---------------LHTIERVYVSLTTGVPSPPQGRI 326

Query: 323 SAPLSKVLLDDGKTERVVLANRTSLEASQEAITEYRVLGPTINGCS-WIELCPRTSRKHQ 369
             P+ +      +     +        ++ A + Y+V+     G S  +E    T R HQ
Sbjct: 327 EIPIGRD--SSNRIRMAAIPGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQ 358

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022997125.11.0e-26293.25RNA pseudouridine synthase 3, mitochondrial [Cucurbita maxima] >XP_022997126.1 R... [more]
XP_022961737.11.9e-26193.04RNA pseudouridine synthase 3, mitochondrial [Cucurbita moschata] >XP_022961738.1... [more]
XP_022131827.12.5e-26193.25RNA pseudouridine synthase 3, mitochondrial isoform X3 [Momordica charantia][more]
XP_023545207.11.4e-25992.62RNA pseudouridine synthase 3, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023... [more]
XP_038885249.13.2e-25692.31LOW QUALITY PROTEIN: RNA pseudouridine synthase 3, mitochondrial [Benincasa hisp... [more]
Match NameE-valueIdentityDescription
Q5XET61.4e-19369.56RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q0J4D43.9e-17261.20RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q9LT724.9e-7435.85RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q69K074.3e-4637.72RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=3... [more]
Q28C591.7e-2631.34Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1K8P85.0e-26393.25RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1HAX89.4e-26293.04RNA pseudouridine synthase 3, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1BS401.2e-26193.25RNA pseudouridine synthase 3, mitochondrial isoform X3 OS=Momordica charantia OX... [more]
A0A6J1BQS05.3e-24989.26RNA pseudouridine synthase 3, mitochondrial isoform X1 OS=Momordica charantia OX... [more]
A0A0A0LQ041.7e-24788.05PseudoU_synth_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G40... [more]
Match NameE-valueIdentityDescription
AT1G78910.19.8e-19569.56Pseudouridine synthase family protein [more]
AT3G19440.13.5e-7535.85Pseudouridine synthase family protein [more]
AT1G76050.26.7e-1021.56Pseudouridine synthase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006145Pseudouridine synthase, RsuA/RluA-likePFAMPF00849PseudoU_synth_2coord: 183..354
e-value: 4.5E-22
score: 78.9
IPR006145Pseudouridine synthase, RsuA/RluA-likeCDDcd02869PseudoU_synth_RluA_likecoord: 183..368
e-value: 8.23793E-42
score: 145.173
NoneNo IPR availableGENE3D3.30.2350.10Pseudouridine synthasecoord: 169..431
e-value: 2.2E-47
score: 163.5
NoneNo IPR availablePANTHERPTHR21600MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASEcoord: 6..471
NoneNo IPR availablePANTHERPTHR21600:SF53RNA PSEUDOURIDINE SYNTHASE 3, MITOCHONDRIALcoord: 6..471
IPR020103Pseudouridine synthase, catalytic domain superfamilySUPERFAMILY55120Pseudouridine synthasecoord: 169..425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0004805.1Lag0004805.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000455 enzyme-directed rRNA pseudouridine synthesis
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0009451 RNA modification
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding