Lag0004045 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0004045
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	increased DNA methylation 3-like
Location	chr6: 685496 .. 686049 (+)
RNA-Seq Expression	Lag0004045
Synteny	Lag0004045

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACATGGAAGGCCATGGCGATCACACGAAACTGAAGCCATCAGTTATTTTAACAGGAACAGCCAAGGAAGGTAGTTCTGGCCCTCCTATTGGTCTTGTTGATATCGGTGTGAGCGAAGGTGCATACCTTTTTCGCGTTGCACTGCCTGGTGTACGGAAGGATCGAAGTAAGCACCTTGTTTTGTCTTATTTTTCTATCAATCTAGCATTTGCATGGGACTGATCCATTATATTATGCGTTTGCTTATTAGAGGGATAACCAACTAGACATCTCTTCCAGGTAAGGTAAAATTTGAGATCAAAAGCGATGGTAGAGTTCAGATTGAAGGAGTGATGACAGGTCCAGGCCTTTTAAAAGAATCATCAGCTTTGTATCAGATGAAAGTTCAGCAACTGTGTCCTCCGGGACCTTTTACCGTTTCTTTTAAGCTGCCTGGACCGGTTGATCCGCGGTTGTCTTCTCCTAGTTTCCGGCCTGATGGCATTCTGGAAGTGGTGGTTATGAAGTCCAGAGCACCTCCAACAGTGGCTGATTCCTACCCACCATTGTGA

mRNA sequence

ATGGACATGGAAGGCCATGGCGATCACACGAAACTGAAGCCATCAGTTATTTTAACAGGAACAGCCAAGGAAGGTAGTTCTGGCCCTCCTATTGGTCTTGTTGATATCGGTGTGAGCGAAGGTGCATACCTTTTTCGCGTTGCACTGCCTGGTGTACGGAAGGATCGAAGTAAGGTAAAATTTGAGATCAAAAGCGATGGTAGAGTTCAGATTGAAGGAGTGATGACAGGTCCAGGCCTTTTAAAAGAATCATCAGCTTTGTATCAGATGAAAGTTCAGCAACTGTGTCCTCCGGGACCTTTTACCGTTTCTTTTAAGCTGCCTGGACCGGTTGATCCGCGGTTGTCTTCTCCTAGTTTCCGGCCTGATGGCATTCTGGAAGTGGTGGTTATGAAGTCCAGAGCACCTCCAACAGTGGCTGATTCCTACCCACCATTGTGA

Coding sequence (CDS)

ATGGACATGGAAGGCCATGGCGATCACACGAAACTGAAGCCATCAGTTATTTTAACAGGAACAGCCAAGGAAGGTAGTTCTGGCCCTCCTATTGGTCTTGTTGATATCGGTGTGAGCGAAGGTGCATACCTTTTTCGCGTTGCACTGCCTGGTGTACGGAAGGATCGAAGTAAGGTAAAATTTGAGATCAAAAGCGATGGTAGAGTTCAGATTGAAGGAGTGATGACAGGTCCAGGCCTTTTAAAAGAATCATCAGCTTTGTATCAGATGAAAGTTCAGCAACTGTGTCCTCCGGGACCTTTTACCGTTTCTTTTAAGCTGCCTGGACCGGTTGATCCGCGGTTGTCTTCTCCTAGTTTCCGGCCTGATGGCATTCTGGAAGTGGTGGTTATGAAGTCCAGAGCACCTCCAACAGTGGCTGATTCCTACCCACCATTGTGA

Protein sequence

MDMEGHGDHTKLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFEIKSDGRVQIEGVMTGPGLLKESSALYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRPDGILEVVVMKSRAPPTVADSYPPL

Homology

[Close]

BLAST of Lag0004045 vs. NCBI nr
Match: XP_022951227.1 (increased DNA methylation 3-like [Cucurbita moschata] >XP_022951228.1 increased DNA methylation 3-like [Cucurbita moschata] >XP_022951229.1 increased DNA methylation 3-like [Cucurbita moschata] >XP_022951230.1 increased DNA methylation 3-like [Cucurbita moschata] >XP_023538561.1 increased DNA methylation 3-like [Cucurbita pepo subsp. pepo] >XP_023538562.1 increased DNA methylation 3-like [Cucurbita pepo subsp. pepo] >XP_023538563.1 increased DNA methylation 3-like [Cucurbita pepo subsp. pepo] >XP_023538564.1 increased DNA methylation 3-like [Cucurbita pepo subsp. pepo] >XP_023538565.1 increased DNA methylation 3-like [Cucurbita pepo subsp. pepo] >KAG6585797.1 Increased DNA methylation 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 279.3 bits (713), Expect = 2.0e-71
Identity = 138/145 (95.17%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 1   MDMEGHGDHTKLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVK 60
           M MEGHGDH KLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVK
Sbjct: 1   MVMEGHGDHMKLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVK 60

Query: 61  FEIKSDGRVQIEGVMTGPGLLKESSALYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSF 120
           FEIKSDGRV IEGVMTGPGLLK+SSA+YQMKVQQLCPPG FTVSFKLPGPVDPRLSSPSF
Sbjct: 61  FEIKSDGRVHIEGVMTGPGLLKDSSAMYQMKVQQLCPPGSFTVSFKLPGPVDPRLSSPSF 120

Query: 121 RPDGILEVVVMKSRAPPTVADSYPP 146
           RPDGILEVVVMKSRAPPTVADS+PP
Sbjct: 121 RPDGILEVVVMKSRAPPTVADSHPP 145

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BLAST of Lag0004045 vs. NCBI nr
Match: XP_023002579.1 (increased DNA methylation 3-like [Cucurbita maxima] >XP_023002580.1 increased DNA methylation 3-like [Cucurbita maxima] >XP_023002581.1 increased DNA methylation 3-like [Cucurbita maxima] >XP_023002582.1 increased DNA methylation 3-like [Cucurbita maxima])

HSP 1 Score: 278.1 bits (710), Expect = 4.4e-71
Identity = 138/145 (95.17%), Postives = 140/145 (96.55%), Query Frame = 0


Query: 1   MDMEGHGDHTKLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVK 60
           M MEGHGDH KLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVK
Sbjct: 1   MVMEGHGDHMKLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVK 60

Query: 61  FEIKSDGRVQIEGVMTGPGLLKESSALYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSF 120
           FEIKSDGRV IEGVMTGPGLLK+SSA+YQMKVQQLCPPG FTVSFKLPGPVDPRLSSPSF
Sbjct: 61  FEIKSDGRVHIEGVMTGPGLLKDSSAMYQMKVQQLCPPGSFTVSFKLPGPVDPRLSSPSF 120

Query: 121 RPDGILEVVVMKSRAPPTVADSYPP 146
           RPDGILEVVVMKSRAPPTVADS PP
Sbjct: 121 RPDGILEVVVMKSRAPPTVADSLPP 145

[Close]

BLAST of Lag0004045 vs. NCBI nr
Match: XP_038884247.1 (increased DNA methylation 3 [Benincasa hispida] >XP_038884248.1 increased DNA methylation 3 [Benincasa hispida] >XP_038884249.1 increased DNA methylation 3 [Benincasa hispida] >XP_038884250.1 increased DNA methylation 3 [Benincasa hispida] >XP_038884251.1 increased DNA methylation 3 [Benincasa hispida])

HSP 1 Score: 272.3 bits (695), Expect = 2.4e-69
Identity = 135/144 (93.75%), Postives = 139/144 (96.53%), Query Frame = 0


Query: 3   MEGHGDHTKLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFE 62
           MEGHGD T  KPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFE
Sbjct: 1   MEGHGDQTLRKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFE 60

Query: 63  IKSDGRVQIEGVMTGPGLLKESSALYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRP 122
           IKSDG+VQIEGVMTGPG+LKESSA+YQMKVQQLCPPGPFTVSFKLPGPVDPRLS PSFRP
Sbjct: 61  IKSDGKVQIEGVMTGPGVLKESSAMYQMKVQQLCPPGPFTVSFKLPGPVDPRLSLPSFRP 120

Query: 123 DGILEVVVMKSRAPPTVADSYPPL 147
           DGILEVVVMKSRA PTVADS+PPL
Sbjct: 121 DGILEVVVMKSRAHPTVADSHPPL 144

[Close]

BLAST of Lag0004045 vs. NCBI nr
Match: XP_008464812.1 (PREDICTED: increased DNA methylation 3 isoform X2 [Cucumis melo] >XP_008464813.1 PREDICTED: increased DNA methylation 3 isoform X2 [Cucumis melo] >XP_008464815.1 PREDICTED: increased DNA methylation 3 isoform X2 [Cucumis melo] >KAA0045408.1 increased DNA methylation 3 isoform X2 [Cucumis melo var. makuwa] >TYK11329.1 increased DNA methylation 3 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 271.9 bits (694), Expect = 3.1e-69
Identity = 135/144 (93.75%), Postives = 139/144 (96.53%), Query Frame = 0


Query: 3   MEGHGDHTKLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFE 62
           MEG+GD T LKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFE
Sbjct: 1   MEGNGDQTMLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFE 60

Query: 63  IKSDGRVQIEGVMTGPGLLKESSALYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRP 122
           IKSDG+VQIEGVM+GPG LKESSA+YQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRP
Sbjct: 61  IKSDGKVQIEGVMSGPGFLKESSAMYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRP 120

Query: 123 DGILEVVVMKSRAPPTVADSYPPL 147
           DGILEVVVMKSRA PTVADS PPL
Sbjct: 121 DGILEVVVMKSRAQPTVADSQPPL 144

[Close]

BLAST of Lag0004045 vs. NCBI nr
Match: XP_004144249.1 (increased DNA methylation 3 [Cucumis sativus] >XP_004144250.1 increased DNA methylation 3 [Cucumis sativus] >XP_011657329.1 increased DNA methylation 3 [Cucumis sativus] >XP_031742703.1 increased DNA methylation 3 [Cucumis sativus] >XP_031742704.1 increased DNA methylation 3 [Cucumis sativus] >XP_031742705.1 increased DNA methylation 3 [Cucumis sativus] >XP_031742706.1 increased DNA methylation 3 [Cucumis sativus] >XP_031742707.1 increased DNA methylation 3 [Cucumis sativus] >XP_031742708.1 increased DNA methylation 3 [Cucumis sativus] >XP_031742709.1 increased DNA methylation 3 [Cucumis sativus] >XP_031742710.1 increased DNA methylation 3 [Cucumis sativus] >XP_031742711.1 increased DNA methylation 3 [Cucumis sativus] >XP_031742712.1 increased DNA methylation 3 [Cucumis sativus] >KGN47518.1 hypothetical protein Csa_018965 [Cucumis sativus])

HSP 1 Score: 271.9 bits (694), Expect = 3.1e-69
Identity = 135/144 (93.75%), Postives = 139/144 (96.53%), Query Frame = 0


Query: 3   MEGHGDHTKLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFE 62
           MEG+GD T LKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFE
Sbjct: 1   MEGNGDQTMLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFE 60

Query: 63  IKSDGRVQIEGVMTGPGLLKESSALYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRP 122
           IKSDG+VQIEGVM+GPG LKESSA+YQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRP
Sbjct: 61  IKSDGKVQIEGVMSGPGFLKESSAMYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRP 120

Query: 123 DGILEVVVMKSRAPPTVADSYPPL 147
           DGILEVVVMKSRA PTVADS PPL
Sbjct: 121 DGILEVVVMKSRAQPTVADSQPPL 144

[Close]

BLAST of Lag0004045 vs. ExPASy Swiss-Prot
Match: Q9SYQ0 (Increased DNA methylation 3 OS=Arabidopsis thaliana OX=3702 GN=IDM3 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 3.5e-31
Identity = 65/123 (52.85%), Postives = 91/123 (73.98%), Query Frame = 0


Query: 13  KPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFEIKSDGRVQIE 72
           +PSV+ TGTA + + G  +G+VDIGV++ AY F+VALPGVRKD  +   EI+SDG+V +E
Sbjct: 339 EPSVVTTGTASKETLGSSVGVVDIGVNKVAYFFQVALPGVRKDYGEFSCEIESDGKVILE 398

Query: 73  GVMT-GPGLLKESSALYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRPDGILEVVVM 132
           G  T G   +K  S +++M +++LCPPGPF + F LPGPVDPRL SP+FR DGI E V++
Sbjct: 399 GSTTRGEKNIKRHSRVFEMNIRKLCPPGPFKLCFNLPGPVDPRLFSPNFRSDGIFEGVII 458

Query: 133 KSR 135
           + +
Sbjct: 459 RHK 461

[Close]

BLAST of Lag0004045 vs. ExPASy Swiss-Prot
Match: Q84K79 (Alpha-crystallin domain-containing protein 22.3 OS=Arabidopsis thaliana OX=3702 GN=ACD22.3 PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 2.7e-23
Identity = 57/124 (45.97%), Postives = 79/124 (63.71%), Query Frame = 0


Query: 10  TKLKPSVILTGTAKEGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFEIKSDGRV 69
           ++ K  V LTG+A  G  G  IGLVDI  SE +Y FRVALPGV +D  +   EI+ DG++
Sbjct: 78  SQTKTGVALTGSAAMGKIGLTIGLVDIAESEDSYYFRVALPGVSRDEKEFSCEIEPDGKI 137

Query: 70  QIEG-VMTGPGLLKESSALYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRPDGILEV 129
            I+G   TG   + + + +++M  Q LCPPG FT++F+LPGPV     + +F  DG+LE 
Sbjct: 138 MIKGATTTGEQTVCKHNQIFKMLTQNLCPPGHFTINFQLPGPVSNEEFNGNFGSDGVLEG 197

Query: 130 VVMK 133
           VV K
Sbjct: 198 VVKK 201

[Close]

BLAST of Lag0004045 vs. ExPASy Swiss-Prot
Match: Q8RWL4 (Increased DNA methylation 2 OS=Arabidopsis thaliana OX=3702 GN=IDM2 PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.3e-22
Identity = 56/117 (47.86%), Postives = 77/117 (65.81%), Query Frame = 0


Query: 24  EGSSGPPIGLVDIGVSEGAYLFRVALPGVRKDRSKVKFEIKSDGRVQIEGV-MTGPGLLK 83
           E   G  +GL+DIG  + AYLFRV+LPGV++D      E++ +G+V + GV  TG   +K
Sbjct: 233 EAHGGLMVGLMDIGECDDAYLFRVSLPGVKRDERYFSCEVEDNGKVLVRGVTTTGGKRVK 292

Query: 84  ESSALYQMKVQQLCPPGPFTVSFKLPGPVDPRLSSPSFRPDGILEVVVMKSRAPPTV 140
             S +++M+ + LCPPG F+VSF+LPGPV P   S +F  DGILE VVMK+    TV
Sbjct: 293 RYSHVFEMQTRSLCPPGNFSVSFRLPGPVHPHEFSGNFGTDGILEGVVMKNLQKQTV 349

The following BLAST results are available for this feature:

BLAST of Lag0004045 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022951227.1	2.0e-71	95.17	increased DNA methylation 3-like [Cucurbita moschata] >XP_022951228.1 increased ...	[more]
XP_023002579.1	4.4e-71	95.17	increased DNA methylation 3-like [Cucurbita maxima] >XP_023002580.1 increased DN...	[more]
XP_038884247.1	2.4e-69	93.75	increased DNA methylation 3 [Benincasa hispida] >XP_038884248.1 increased DNA me...	[more]
XP_008464812.1	3.1e-69	93.75	PREDICTED: increased DNA methylation 3 isoform X2 [Cucumis melo] >XP_008464813.1...	[more]
XP_004144249.1	3.1e-69	93.75	increased DNA methylation 3 [Cucumis sativus] >XP_004144250.1 increased DNA meth...	[more]

BLAST of Lag0004045 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SYQ0	3.5e-31	52.85	Increased DNA methylation 3 OS=Arabidopsis thaliana OX=3702 GN=IDM3 PE=1 SV=1	[more]
Q84K79	2.7e-23	45.97	Alpha-crystallin domain-containing protein 22.3 OS=Arabidopsis thaliana OX=3702 ...	[more]
Q8RWL4	1.3e-22	47.86	Increased DNA methylation 2 OS=Arabidopsis thaliana OX=3702 GN=IDM2 PE=1 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 33..142 e-value: 1.2E-11 score: 46.4
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 32..135
IPR039321	Increased DNA methylation 2/3-like	PANTHER	PTHR34661	INCREASED DNA METHYLATION 3	coord: 1..138
None	No IPR available	PANTHER	PTHR34661:SF2	INCREASED DNA METHYLATION 3-LIKE	coord: 1..138
None	No IPR available	CDD	cd06464	ACD_sHsps-like	coord: 35..132 e-value: 2.64248E-10 score: 51.7861
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 22..146 score: 12.007298

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0004045.1	Lag0004045.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1901537	positive regulation of DNA demethylation
cellular_component	GO:0005634	nucleus