Lag0029063 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0029063 vs. NCBI nr
Match: KAG6602186.1 (UPF0496 protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 516.9 bits (1330), Expect = 1.5e-142
Identity = 283/382 (74.08%), Postives = 312/382 (81.68%), Query Frame = 0

Query: 1   MWAKFRASKIRNSTKLFPSILILDCSENLCNFSQPVLISDFADGKQQIRNKSQKSFNVND 60
           MW KFRAS+                          + IS FAD K QIRNK QKSFNVN+
Sbjct: 1   MWTKFRASRF-------------------------IWISVFADVKHQIRNKPQKSFNVNE 60

Query: 61  EYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILES 120
           EYLC+L ++S++EFF K + IV ESPPSTSSS+    RKFSETILLQ  QLEA PSILES
Sbjct: 61  EYLCALRSRSFVEFFAKAQLIVQESPPSTSSSADLYCRKFSETILLQSDQLEANPSILES 120

Query: 121 PLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSSAEKI 180
           PLL+M+PEL+GLL D+FNVSAEASNLC+RLLANLK ARSNSRF+Q+ LDSIEKCSS ++I
Sbjct: 121 PLLVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEI 180

Query: 181 ETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGL 240
           ETI SDLLAQRAP SD+DKRDFA IHD+Y+AVS  LN +RKKVARKIRSIKIIDRFTCGL
Sbjct: 181 ETITSDLLAQRAPLSDLDKRDFARIHDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGL 240

Query: 241 IAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYI 300
           +AITSR LTALVMAA  GP    FRLKS RRKLLRHQML NGGLERVG QLE AAKGSYI
Sbjct: 241 VAITSRGLTALVMAADQGPVLRYFRLKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDH KAMVRLFVERKEDKFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDV 357

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+C VTINR+R  VINQM
Sbjct: 361 EEHLYLCFVTINRSRASVINQM 357

BLAST of Lag0029063 vs. NCBI nr
Match: XP_022959620.1 (UPF0496 protein 3-like [Cucurbita moschata])

HSP 1 Score: 516.5 bits (1329), Expect = 1.9e-142
Identity = 283/382 (74.08%), Postives = 313/382 (81.94%), Query Frame = 0

Query: 1   MWAKFRASKIRNSTKLFPSILILDCSENLCNFSQPVLISDFADGKQQIRNKSQKSFNVND 60
           MW KFRAS+                          + IS FAD K QIRNK QKSFNVN+
Sbjct: 1   MWTKFRASRF-------------------------IWISVFADVKHQIRNKPQKSFNVNE 60

Query: 61  EYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILES 120
           EYLC+L ++S++EFF K + IV ESPPSTSSS+    RKFSETILLQ  QLEA PSILES
Sbjct: 61  EYLCALRSRSFVEFFAKAQLIVQESPPSTSSSADLYCRKFSETILLQSDQLEANPSILES 120

Query: 121 PLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSSAEKI 180
           PLL+M+PEL+GLL D+FNVSAEASNLC+RLLANLK ARSNSRF+Q+ LDSIEKCSS ++I
Sbjct: 121 PLLVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEI 180

Query: 181 ETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGL 240
           ETI SDLLAQRAP SD+DKRDFA IHD+Y+AVS  LN +RKKVARKIRSIKIIDRFTCGL
Sbjct: 181 ETITSDLLAQRAPLSDLDKRDFARIHDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGL 240

Query: 241 IAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYI 300
           +AITSR LTALVMAA  GP    FRLKS RRKLLRHQML NGGLERVG QLE AAKGSYI
Sbjct: 241 VAITSRGLTALVMAADQGPVLRYFRLKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDH KAMVRLFVERKE+KFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKENKFAVAVAMDEMKRSNVSIRKQVEDV 357

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+CIVTINR+R  VINQM
Sbjct: 361 EEHLYLCIVTINRSRASVINQM 357

BLAST of Lag0029063 vs. NCBI nr
Match: KAG7032870.1 (UPF0496 protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 515.4 bits (1326), Expect = 4.2e-142
Identity = 282/382 (73.82%), Postives = 311/382 (81.41%), Query Frame = 0

Query: 1   MWAKFRASKIRNSTKLFPSILILDCSENLCNFSQPVLISDFADGKQQIRNKSQKSFNVND 60
           MW KFRAS+                          + IS FAD K QIRNK QKSFNVN+
Sbjct: 1   MWTKFRASRF-------------------------IWISVFADVKHQIRNKPQKSFNVNE 60

Query: 61  EYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILES 120
           EYLC+L ++S++EFF K + IV ESPPSTSSS+    RKFSETILLQ  QLEA PSILES
Sbjct: 61  EYLCALRSRSFVEFFAKAQLIVQESPPSTSSSADLYCRKFSETILLQSDQLEANPSILES 120

Query: 121 PLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSSAEKI 180
           PLL+M+PEL+GLL D+FNVSAEASNLC+RLL NLK ARSNSRF+Q+ LDSIEKCSS ++I
Sbjct: 121 PLLVMLPELRGLLIDFFNVSAEASNLCHRLLVNLKLARSNSRFVQKLLDSIEKCSSPKEI 180

Query: 181 ETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGL 240
           ETI SDLLAQRAP SD+DKRDFA IHD+Y+AVS  LN +RKKVARKIRSIKIIDRFTCGL
Sbjct: 181 ETITSDLLAQRAPLSDLDKRDFARIHDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGL 240

Query: 241 IAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYI 300
           +AITSR LTALVMAA  GP    FRLKS RRKLLRHQML NGGLERVG QLE AAKGSYI
Sbjct: 241 VAITSRGLTALVMAADQGPVLRYFRLKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDH KAMVRLFVERKEDKFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDV 357

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+C VTINR+R  VINQM
Sbjct: 361 EEHLYLCFVTINRSRASVINQM 357

BLAST of Lag0029063 vs. NCBI nr
Match: XP_023542867.1 (UPF0496 protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 511.9 bits (1317), Expect = 4.7e-141
Identity = 279/382 (73.04%), Postives = 311/382 (81.41%), Query Frame = 0

Query: 1   MWAKFRASKIRNSTKLFPSILILDCSENLCNFSQPVLISDFADGKQQIRNKSQKSFNVND 60
           MW KFRAS+                          + IS FAD K QIRNK Q+SFNVN+
Sbjct: 1   MWTKFRASRF-------------------------IWISVFADVKHQIRNKPQRSFNVNE 60

Query: 61  EYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILES 120
           EYLC+L ++S++EFF K + IV ESPPSTSSS+    RKFSETILLQ  QLEA PSILES
Sbjct: 61  EYLCALRSRSFVEFFAKAQLIVQESPPSTSSSADHYCRKFSETILLQSDQLEANPSILES 120

Query: 121 PLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSSAEKI 180
           PLL+M+PEL+GLL D+FNVSAEASNLC+RLLANLK ARSNSRF+Q+ LDSIEKC S ++I
Sbjct: 121 PLLVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCCSPKEI 180

Query: 181 ETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGL 240
           ETI SDLLAQRAP SD+DKRDFA IHD Y+AVS  LN +RKKVARKIRSIKIIDR+TCGL
Sbjct: 181 ETITSDLLAQRAPLSDLDKRDFARIHDNYAAVSRSLNITRKKVARKIRSIKIIDRYTCGL 240

Query: 241 IAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYI 300
           +AITSR LTALV+AA  GP    FRLKS RRKLL+HQML NGGLERVG QLE AAKGSYI
Sbjct: 241 VAITSRGLTALVLAADQGPVLRYFRLKSIRRKLLQHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDH KAMVRLFVERKEDKFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDV 357

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+CIVTINR+R  VINQM
Sbjct: 361 EEHLYLCIVTINRSRASVINQM 357

BLAST of Lag0029063 vs. NCBI nr
Match: XP_038884256.1 (UPF0496 protein At3g49070-like [Benincasa hispida])

HSP 1 Score: 511.1 bits (1315), Expect = 8.0e-141
Identity = 277/386 (71.76%), Postives = 318/386 (82.38%), Query Frame = 0

Query: 1   MWAKFRASKIRNSTKLFPSILILDCSENLCNFS--QPVLISDFADGKQQIRNKSQKSFNV 60
           MW KFR S+IRNSTKL  SI+   C  +LCNFS  QPV IS FAD +Q+I  K QKSFNV
Sbjct: 1   MWTKFRHSEIRNSTKLSQSIIA--CFAHLCNFSQTQPVFISIFADEEQEIGKKPQKSFNV 60

Query: 61  NDEYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSS--TDLRRKFSETILLQPGQLEAIPS 120
           N+EYLC+L TKS+ EFFTK   IV ESPPS +SSS   D  RK SETILLQP +LEA+PS
Sbjct: 61  NEEYLCTLRTKSFSEFFTKAEFIVDESPPSITSSSAAADRCRKLSETILLQPVRLEAVPS 120

Query: 121 ILESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSS 180
           ILES  LLM+PELKGL  DY NVSA+ASNLC RLL N+KS RS SR IQQSLDSIEKC S
Sbjct: 121 ILESSFLLMLPELKGLFIDYLNVSAKASNLCTRLLTNVKSTRSKSRCIQQSLDSIEKCFS 180

Query: 181 AEKIETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRF 240
           +E IE+IASDLL+ RAPFSD+DKRDF LIH +Y+A+S RLNC+RKKV RKIR+IKIID  
Sbjct: 181 SETIESIASDLLSLRAPFSDLDKRDFTLIHADYTAISRRLNCTRKKVERKIRTIKIIDGI 240

Query: 241 TCGLIAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAK 300
           TCGL +IT+RTLT  VMA  PGP  FG+RLKS  RKL+RH+MLRNGGLE+VG ++E AAK
Sbjct: 241 TCGLNSITTRTLTDSVMAKDPGPVIFGYRLKSLGRKLVRHEMLRNGGLEKVGEKVEAAAK 300

Query: 301 GSYILNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQ 360
           GSYI+ RELDTTSRLVVRLGDAVDH KAM+RLF  RKEDKFAV+VAMDE+KK+N+S+RKQ
Sbjct: 301 GSYIMKRELDTTSRLVVRLGDAVDHGKAMLRLFGGRKEDKFAVSVAMDEVKKNNVSMRKQ 360

Query: 361 VEDVEEHLYMCIVTINRTRTLVINQM 383
           VEDVEEHLY+CIVTINR+R  VI Q+
Sbjct: 361 VEDVEEHLYLCIVTINRSRASVIKQL 384

BLAST of Lag0029063 vs. ExPASy Swiss-Prot
Match: Q6DYE5 (UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2)

HSP 1 Score: 112.5 bits (280), Expect = 1.1e-23
Identity = 104/371 (28.03%), Postives = 183/371 (49.33%), Query Frame = 0

Query: 45  KQQIRNKSQKSFNVNDEYLCSLGTKSYIEFFTKVR--------SIVHESPPSTSSSSTDL 104
           K+  R+ + K  +VN+EY  +  T SY+E  TK          S +  S PS SSSS   
Sbjct: 24  KECCRSLTSK-LSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLS 83

Query: 105 RRKFSETILLQPGQLEAIPSILESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKS 164
                   LL P Q E + ++++         L  L+  +F++S+EA ++C  LL  L+ 
Sbjct: 84  FHSHFTDYLLDPPQ-ETLDALMQD------SSLDNLIVTFFDLSSEACDVCETLLQCLQQ 143

Query: 165 ARSNSRFIQQSLDSIEK-CSSAEKIETIASDL-------LAQRAPFSD-----IDKRDFA 224
            + N   I++ +   ++ C+ A+ +E     L       L++ A   +     +++  F 
Sbjct: 144 IKINHNKIKRVMKIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFR 203

Query: 225 LIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGLIAIT--SRTLTALVMA------A 284
           ++HD  S +  +L   ++++ RKIR  K   +     + IT  +  +T L++A       
Sbjct: 204 IVHDANSDLLTKLTSKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILGV 263

Query: 285 GPGPAHFGF----RLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYILNRELDTTSRL 344
              PA  G      L+  + K   H+  ++  LE++G Q++ AAKG +IL  +LDT SRL
Sbjct: 264 FAAPALLGLCSFCLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRL 323

Query: 345 VVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTI 383
             RL D ++HRK +  +  + ++ +  +  A+ E          Q++++EEHLY+C  TI
Sbjct: 324 AGRLCDEIEHRKTVAAMCAKSRKIE-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTI 383

BLAST of Lag0029063 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 1.4e-23
Identity = 103/353 (29.18%), Postives = 170/353 (48.16%), Query Frame = 0

Query: 55  SFNVNDEYLCSLGTKSYIEFFTKVRSIV----------HESPPSTSSSSTDLRRKFSETI 114
           SF+  +EY  +  T+SY +F+ +V  I           H      ++S      +     
Sbjct: 31  SFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEH 90

Query: 115 LLQPGQLEAIPSILESPL-LLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRF 174
           LL+P Q  A+ + L SP    + P+++GLL  Y+  +A AS LC+ LL +++  R   R 
Sbjct: 91  LLEPDQ-RAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRP 150

Query: 175 IQQSLDSIEKCSSAEKIETIASDLLAQRAPFSDIDKRDFAL--IHDEYSAVSYRLNCSRK 234
           ++ +L    K +S   +  +A    A   PF+ +      L  +      +   L+  RK
Sbjct: 151 LKHTL---RKLASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDAGRK 210

Query: 235 KVARKIRSIKIIDRFTCGLIAITSRTLTALVMAAGPGPAHFGFRLKSFRR------KLLR 294
           K   +IRS+  + R      A++   +TA+ + A  G       L +F          L 
Sbjct: 211 KARHRIRSVARLRR------ALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLG 270

Query: 295 HQMLRNGGLERVGAQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKED 354
            +        R   QLE AAKG+YILNR+++T SRLV R+ D  +H  A++RL VE +  
Sbjct: 271 ERFFSGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALLRLCVEHRPA 330

Query: 355 KFA------VAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
             A      V   + +L K+  S R+Q++++EEHL++C +TIN+ R +V+N M
Sbjct: 331 AGAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTINKARIMVMNFM 373

BLAST of Lag0029063 vs. ExPASy Swiss-Prot
Match: Q10RR9 (UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.6e-22
Identity = 102/353 (28.90%), Postives = 168/353 (47.59%), Query Frame = 0

Query: 55  SFNVNDEYLCSLGTKSYIEFFTKVRSIV----------HESPPSTSSSSTDLRRKFSETI 114
           SF+  +EY  +  T+SY +F+ +V  I           H      ++S      +     
Sbjct: 31  SFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEH 90

Query: 115 LLQPGQLEAIPSILESPL-LLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRF 174
           LL+P Q  A+ + L SP    + P+++GLL  Y+  +A AS LC+ LL +++  R   R 
Sbjct: 91  LLEPDQ-RAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRP 150

Query: 175 IQQSLDSIEKCSSAEKIETIASDLLAQRAPFSDIDKRDFAL--IHDEYSAVSYRLNCSRK 234
           ++ +L    K +S   +  +A    A   PF+ +      L  +      +   L+  RK
Sbjct: 151 LKHTL---RKLASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDAGRK 210

Query: 235 KVARKIRSIKIIDRFTCGLIAITSRTLTALVMAAGPGPAHFGFRLKSFRR------KLLR 294
           K   +IRS+  + R      A++   +TA+ + A  G       L +F          L 
Sbjct: 211 KARHRIRSVARLRR------ALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLG 270

Query: 295 HQMLRNGGLERVGAQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKED 354
            +        R   QLE AAKG+YILNR+++T SRLV R+ D  +H  A+ RL VE +  
Sbjct: 271 ERFFSGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALRRLCVEHRPA 330

Query: 355 KFA------VAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
             A      V   + +L K+  S R+Q++++EEHL++C +T N+ R +V+N M
Sbjct: 331 AGAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTTNKARIMVMNFM 373

BLAST of Lag0029063 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 3.0e-21
Identity = 108/349 (30.95%), Postives = 167/349 (47.85%), Query Frame = 0

Query: 51  KSQKSFNVNDEYLCSLGTKSYIEFFTKVRSIVHE--------SPPSTSSSSTDLR----R 110
           K     +V +EY  +  T+SY  F+T+V  +  +        S P   SSST  R    R
Sbjct: 64  KDGADVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYR 123

Query: 111 KFSETILLQPGQLEAIPSILESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSAR 170
            F+   LL P  L  I  IL+  +  +    + LL+DYF  +A A  LC +LL N+   R
Sbjct: 124 LFAHN-LLDP-DLNTITRILD--VSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLR 183

Query: 171 SNSRFIQQSLDSIEKCSSAEKIETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNC 230
           S    ++    S E  +S   I+   +++     PF     R   LI      +  RL  
Sbjct: 184 SKYESLKPKFHS-ENHNSLALIDQF-TEISKWFDPFISSGSR-IQLIRSGCLYLLKRLES 243

Query: 231 SRKKVARKIRSIKIIDRFTCGLIAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQM 290
            R K   K++ I  +   +  L+   + TL   +       A   F L      LL  Q 
Sbjct: 244 RRDKTRAKLKLINGLTHSSGLLVLALTTTLIVTI-------ASHAFALFLAAPTLLASQF 303

Query: 291 ----LRNGGLERVGAQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKE 350
               LRN  L +  A+L+ AAKG+YIL+R+LDT SRLV R+ D V+H +AM   +V R  
Sbjct: 304 KPAGLRN-KLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGS 363

Query: 351 DKFAVAVAM-DELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
            +   +  +  ELK+   S  ++++++EEH+Y+C +TINR R L++ ++
Sbjct: 364 GRVRGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEI 397

BLAST of Lag0029063 vs. ExPASy Swiss-Prot
Match: A2YH25 (Putative UPF0496 protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_023618 PE=3 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 3.1e-18
Identity = 72/261 (27.59%), Postives = 135/261 (51.72%), Query Frame = 0

Query: 132 LLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSSAEKIETIASDLLAQR 191
           LL +YF+V+ EA   C+ LLA + +AR +   +++ L  ++     +  + +A  +    
Sbjct: 126 LLIEYFDVTEEACEACSALLAAIGAARRHHLTLRRLLLRLDGGDDDDAKDALARHVRLDN 185

Query: 192 APFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGLI--AITSRTLT 251
            P S     +F  +H   S ++ RL  +++++ R  R+++I        +  A  +  + 
Sbjct: 186 -PLSPGSLSEFHDVHARCSPLASRLAAAQRRLRRLARALRIARGTAAAALVGACAAAIVA 245

Query: 252 ALVMAA------GPGPAHFGFRLKSFRRKLLRH--QMLRNGGLERVGAQLEEAAKGSYIL 311
           A+V+AA      G   A FG       R   R   + + +    R GA L+ AA+G+YI+
Sbjct: 246 AVVLAAHALVGIGVAAAAFGATPAGAARWWARRAAEKVSSRHYARAGATLDAAARGAYIV 305

Query: 312 NRELDTTSRLVVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDVE 371
            R+LDT SR+V R  D ++H + + R+ +    ++  +     E ++    +R Q+ ++E
Sbjct: 306 GRDLDTVSRMVRRAHDELEHGRDVARIAMRGHGERPLLQEVAREEEECEEDLRAQLAELE 365

Query: 372 EHLYMCIVTINRTRTLVINQM 383
           EH+ +C++TINRTR LV ++M
Sbjct: 366 EHVCLCLITINRTRRLVAHEM 385

BLAST of Lag0029063 vs. ExPASy TrEMBL
Match: A0A6J1H6T3 (UPF0496 protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460642 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 9.2e-143
Identity = 283/382 (74.08%), Postives = 313/382 (81.94%), Query Frame = 0

Query: 1   MWAKFRASKIRNSTKLFPSILILDCSENLCNFSQPVLISDFADGKQQIRNKSQKSFNVND 60
           MW KFRAS+                          + IS FAD K QIRNK QKSFNVN+
Sbjct: 1   MWTKFRASRF-------------------------IWISVFADVKHQIRNKPQKSFNVNE 60

Query: 61  EYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILES 120
           EYLC+L ++S++EFF K + IV ESPPSTSSS+    RKFSETILLQ  QLEA PSILES
Sbjct: 61  EYLCALRSRSFVEFFAKAQLIVQESPPSTSSSADLYCRKFSETILLQSDQLEANPSILES 120

Query: 121 PLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSSAEKI 180
           PLL+M+PEL+GLL D+FNVSAEASNLC+RLLANLK ARSNSRF+Q+ LDSIEKCSS ++I
Sbjct: 121 PLLVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEI 180

Query: 181 ETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGL 240
           ETI SDLLAQRAP SD+DKRDFA IHD+Y+AVS  LN +RKKVARKIRSIKIIDRFTCGL
Sbjct: 181 ETITSDLLAQRAPLSDLDKRDFARIHDDYAAVSRSLNITRKKVARKIRSIKIIDRFTCGL 240

Query: 241 IAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYI 300
           +AITSR LTALVMAA  GP    FRLKS RRKLLRHQML NGGLERVG QLE AAKGSYI
Sbjct: 241 VAITSRGLTALVMAADQGPVLRYFRLKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDH KAMVRLFVERKE+KFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKENKFAVAVAMDEMKRSNVSIRKQVEDV 357

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+CIVTINR+R  VINQM
Sbjct: 361 EEHLYLCIVTINRSRASVINQM 357

BLAST of Lag0029063 vs. ExPASy TrEMBL
Match: A0A6J1JMU4 (UPF0496 protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111487265 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 5.6e-140
Identity = 280/382 (73.30%), Postives = 311/382 (81.41%), Query Frame = 0

Query: 1   MWAKFRASKIRNSTKLFPSILILDCSENLCNFSQPVLISDFADGKQQIRNKSQKSFNVND 60
           MW KFRAS+                          + IS FAD K QIRNK QKSFNVN+
Sbjct: 1   MWTKFRASRF-------------------------IWISVFADVKHQIRNKPQKSFNVNE 60

Query: 61  EYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSSTDLRRKFSETILLQPGQLEAIPSILES 120
           EYLC+L ++S++EFF K + IV ESPPSTSS+     RKFSETILLQ  QLEA PSILES
Sbjct: 61  EYLCALRSRSFVEFFAKAQLIVQESPPSTSSTGHHC-RKFSETILLQIDQLEANPSILES 120

Query: 121 PLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSSAEKI 180
           PLL+M+PEL+GLL D+FNVSAEASNLC+RLLANLK ARSNSRF+Q+ LDSIEKCSS ++I
Sbjct: 121 PLLVMLPELRGLLIDFFNVSAEASNLCHRLLANLKLARSNSRFVQKLLDSIEKCSSPKEI 180

Query: 181 ETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGL 240
           ETI SDLLAQRAP SD+DKRDFA IHD+Y+AVS  LN +RKKVARKIRSIKII++ TCGL
Sbjct: 181 ETITSDLLAQRAPLSDLDKRDFARIHDDYAAVSRSLNITRKKVARKIRSIKIINKCTCGL 240

Query: 241 IAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYI 300
           +AITSR LTALVMAA  GP    FRLKS RRKLLRHQML NGGLERVG QLE AAKGSYI
Sbjct: 241 VAITSRGLTALVMAADQGPVLRYFRLKSIRRKLLRHQMLGNGGLERVGEQLEAAAKGSYI 300

Query: 301 LNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDV 360
           LNRE DTTSRLVVRLGDAVDH KAMVRLFVERKEDKFAVAVAMDE+K+SN+SIRKQVEDV
Sbjct: 301 LNREFDTTSRLVVRLGDAVDHGKAMVRLFVERKEDKFAVAVAMDEMKRSNVSIRKQVEDV 356

Query: 361 EEHLYMCIVTINRTRTLVINQM 383
           EEHLY+CIVTINR+R  VINQM
Sbjct: 361 EEHLYLCIVTINRSRASVINQM 356

BLAST of Lag0029063 vs. ExPASy TrEMBL
Match: A0A6J1BWZ5 (UPF0496 protein 3-like OS=Momordica charantia OX=3673 GN=LOC111006097 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 3.9e-133
Identity = 268/384 (69.79%), Postives = 303/384 (78.91%), Query Frame = 0

Query: 1   MWAKFRASKIRNSTKLFPSILILDCSENLCNFSQPVLISDFADGKQQIRNKSQKSFNVND 60
           MW K R  KIRNST +  SI     ++ L NFSQPVLIS FAD KQQIRNK QKSFNVND
Sbjct: 1   MWPKIRDLKIRNSTHISSSIFACSAAQ-LLNFSQPVLISAFADAKQQIRNKPQKSFNVND 60

Query: 61  EYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSSTD--LRRKFSETILLQPGQLEAIPSIL 120
           EY+C+L TKSY+EFF K +S++ ES P ++SSST    RRK S TILL+P Q EAIPSIL
Sbjct: 61  EYICALRTKSYVEFFIKAQSVIEESSPPSTSSSTGRRRRRKLSGTILLEPSQ-EAIPSIL 120

Query: 121 ESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSSAE 180
           ESP LLM+P+LK LL DYFNVSAEAS  C RLL +++  RSNSR IQ+SLDSIE CSS E
Sbjct: 121 ESPFLLMLPDLKSLLIDYFNVSAEASKFCTRLLEDVELTRSNSRSIQKSLDSIENCSSPE 180

Query: 181 KIETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTC 240
            IETIAS+ LA R PFSD DK DFALIHD+Y AVS RLNC+RKKVARKI+SI+II+  +C
Sbjct: 181 TIETIASEFLALREPFSDPDKHDFALIHDDYEAVSRRLNCTRKKVARKIKSIEIIETISC 240

Query: 241 GLIAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGS 300
           GL+AIT+RTLT L          F F  +SFRRKLLR+QM+RNGGL  VG QLE AAKGS
Sbjct: 241 GLVAITARTLTEL----------FRFPPESFRRKLLRYQMIRNGGLGEVGEQLEAAAKGS 300

Query: 301 YILNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVE 360
           YILNRE DTTSRLV RL DA+DH KAM RLFVERKEDKFAV VAMDELKKSNL +R QVE
Sbjct: 301 YILNREFDTTSRLVARLDDAMDHGKAMARLFVERKEDKFAVQVAMDELKKSNLRLRNQVE 360

Query: 361 DVEEHLYMCIVTINRTRTLVINQM 383
           +VEEHLY+CIVTINR R LVINQ+
Sbjct: 361 EVEEHLYLCIVTINRARGLVINQI 372

BLAST of Lag0029063 vs. ExPASy TrEMBL
Match: A0A5D3DGL5 (UPF0496 protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00820 PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 7.3e-124
Identity = 255/384 (66.41%), Postives = 300/384 (78.12%), Query Frame = 0

Query: 1   MWAKFRASKIRNSTKLFPSILILDCSENLCNFSQPVLISDFADGKQQ-IRNKSQKSFNVN 60
           MW KF  SKI NSTKL  SIL                 S FAD + + IR K ++SFNVN
Sbjct: 1   MWTKFSPSKIINSTKLSQSIL---------------ACSTFADQEHEIIRKKHKRSFNVN 60

Query: 61  DEYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSST--DLRRKFSETILLQPGQLEAIPSI 120
           +EYLC+L T+S+ EFF K  S VHESPPST+SSS+  D   +FSETILLQPGQLEA+PSI
Sbjct: 61  EEYLCTLRTESFAEFFLKPESNVHESPPSTTSSSSAADCCWRFSETILLQPGQLEAVPSI 120

Query: 121 LESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSSA 180
           LES  LLM+PELKGL  DYFN+SA+AS+LC RLLAN K  RS SR IQ+SLDSIEKC S+
Sbjct: 121 LESSFLLMLPELKGLFVDYFNLSAQASDLCTRLLANFKLTRSTSRCIQESLDSIEKCFSS 180

Query: 181 EKIETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFT 240
           E +E+IAS+LLA R+PFSD++KRDFALIHD+Y+ +S+RLNC+RKKVARKIRS+KI+D  T
Sbjct: 181 ETVESIASNLLALRSPFSDLEKRDFALIHDDYTTISHRLNCTRKKVARKIRSMKIMDGIT 240

Query: 241 CGLIAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKG 300
           CGL AIT+RTLT LV AA  GP  FG       RKLLRH+MLRNGGLE+VG +LE AAKG
Sbjct: 241 CGLNAITTRTLTDLVKAADRGPGVFG-------RKLLRHEMLRNGGLEKVGEKLEAAAKG 300

Query: 301 SYILNRELDTTSRLVVRLGDAVDHRKAMVRLFVER-KEDKFAVAVAMDELKKSNLSIRKQ 360
           SYIL REL+TTSRLVVRLGDAVD+ KAMVRLF  R KEDKF V VAMDE+KK+N +IRK+
Sbjct: 301 SYILKRELETTSRLVVRLGDAVDNGKAMVRLFGGRKKEDKFGVVVAMDEVKKNNANIRKR 360

Query: 361 VEDVEEHLYMCIVTINRTRTLVIN 381
           VEDVEEHL +CIV INR++  +IN
Sbjct: 361 VEDVEEHLCLCIVAINRSKASMIN 362

BLAST of Lag0029063 vs. ExPASy TrEMBL
Match: A0A1S3B3L4 (UPF0496 protein 3-like OS=Cucumis melo OX=3656 GN=LOC103485615 PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 7.3e-124
Identity = 255/384 (66.41%), Postives = 300/384 (78.12%), Query Frame = 0

Query: 1   MWAKFRASKIRNSTKLFPSILILDCSENLCNFSQPVLISDFADGKQQ-IRNKSQKSFNVN 60
           MW KF  SKI NSTKL  SIL                 S FAD + + IR K ++SFNVN
Sbjct: 1   MWTKFSPSKIINSTKLSQSIL---------------ACSTFADQEHEIIRKKHKRSFNVN 60

Query: 61  DEYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSST--DLRRKFSETILLQPGQLEAIPSI 120
           +EYLC+L T+S+ EFF K  S VHESPPST+SSS+  D   +FSETILLQPGQLEA+PSI
Sbjct: 61  EEYLCTLRTESFAEFFLKPESNVHESPPSTTSSSSAADCCWRFSETILLQPGQLEAVPSI 120

Query: 121 LESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSARSNSRFIQQSLDSIEKCSSA 180
           LES  LLM+PELKGL  DYFN+SA+AS+LC RLLAN K  RS SR IQ+SLDSIEKC S+
Sbjct: 121 LESSFLLMLPELKGLFVDYFNLSAQASDLCTRLLANFKLTRSTSRCIQESLDSIEKCFSS 180

Query: 181 EKIETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFT 240
           E +E+IAS+LLA R+PFSD++KRDFALIHD+Y+ +S+RLNC+RKKVARKIRS+KI+D  T
Sbjct: 181 ETVESIASNLLALRSPFSDLEKRDFALIHDDYTTISHRLNCTRKKVARKIRSMKIMDGIT 240

Query: 241 CGLIAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKG 300
           CGL AIT+RTLT LV AA  GP  FG       RKLLRH+MLRNGGLE+VG +LE AAKG
Sbjct: 241 CGLNAITTRTLTDLVKAADRGPGVFG-------RKLLRHEMLRNGGLEKVGEKLEAAAKG 300

Query: 301 SYILNRELDTTSRLVVRLGDAVDHRKAMVRLFVER-KEDKFAVAVAMDELKKSNLSIRKQ 360
           SYIL REL+TTSRLVVRLGDAVD+ KAMVRLF  R KEDKF V VAMDE+KK+N +IRK+
Sbjct: 301 SYILKRELETTSRLVVRLGDAVDNGKAMVRLFGGRKKEDKFGVVVAMDEVKKNNANIRKR 360

Query: 361 VEDVEEHLYMCIVTINRTRTLVIN 381
           VEDVEEHL +CIV INR++  +IN
Sbjct: 361 VEDVEEHLCLCIVAINRSKASMIN 362

BLAST of Lag0029063 vs. TAIR 10
Match: AT1G20180.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 112.5 bits (280), Expect = 7.7e-25
Identity = 104/371 (28.03%), Postives = 183/371 (49.33%), Query Frame = 0

Query: 45  KQQIRNKSQKSFNVNDEYLCSLGTKSYIEFFTKVR--------SIVHESPPSTSSSSTDL 104
           K+  R+ + K  +VN+EY  +  T SY+E  TK          S +  S PS SSSS   
Sbjct: 24  KECCRSLTSK-LSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLS 83

Query: 105 RRKFSETILLQPGQLEAIPSILESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKS 164
                   LL P Q E + ++++         L  L+  +F++S+EA ++C  LL  L+ 
Sbjct: 84  FHSHFTDYLLDPPQ-ETLDALMQD------SSLDNLIVTFFDLSSEACDVCETLLQCLQQ 143

Query: 165 ARSNSRFIQQSLDSIEK-CSSAEKIETIASDL-------LAQRAPFSD-----IDKRDFA 224
            + N   I++ +   ++ C+ A+ +E     L       L++ A   +     +++  F 
Sbjct: 144 IKINHNKIKRVMKIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFR 203

Query: 225 LIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGLIAIT--SRTLTALVMA------A 284
           ++HD  S +  +L   ++++ RKIR  K   +     + IT  +  +T L++A       
Sbjct: 204 IVHDANSDLLTKLTSKKRRIRRKIRFFKFCKKLGGYSLVITHSAIVITLLIIALHSILGV 263

Query: 285 GPGPAHFGF----RLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYILNRELDTTSRL 344
              PA  G      L+  + K   H+  ++  LE++G Q++ AAKG +IL  +LDT SRL
Sbjct: 264 FAAPALLGLCSFCLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRL 323

Query: 345 VVRLGDAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTI 383
             RL D ++HRK +  +  + ++ +  +  A+ E          Q++++EEHLY+C  TI
Sbjct: 324 AGRLCDEIEHRKTVAAMCAKSRKIE-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTI 383

BLAST of Lag0029063 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 104.4 bits (259), Expect = 2.1e-22
Identity = 108/349 (30.95%), Postives = 167/349 (47.85%), Query Frame = 0

Query: 51  KSQKSFNVNDEYLCSLGTKSYIEFFTKVRSIVHE--------SPPSTSSSSTDLR----R 110
           K     +V +EY  +  T+SY  F+T+V  +  +        S P   SSST  R    R
Sbjct: 64  KDGADVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYR 123

Query: 111 KFSETILLQPGQLEAIPSILESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKSAR 170
            F+   LL P  L  I  IL+  +  +    + LL+DYF  +A A  LC +LL N+   R
Sbjct: 124 LFAHN-LLDP-DLNTITRILD--VSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLR 183

Query: 171 SNSRFIQQSLDSIEKCSSAEKIETIASDLLAQRAPFSDIDKRDFALIHDEYSAVSYRLNC 230
           S    ++    S E  +S   I+   +++     PF     R   LI      +  RL  
Sbjct: 184 SKYESLKPKFHS-ENHNSLALIDQF-TEISKWFDPFISSGSR-IQLIRSGCLYLLKRLES 243

Query: 231 SRKKVARKIRSIKIIDRFTCGLIAITSRTLTALVMAAGPGPAHFGFRLKSFRRKLLRHQM 290
            R K   K++ I  +   +  L+   + TL   +       A   F L      LL  Q 
Sbjct: 244 RRDKTRAKLKLINGLTHSSGLLVLALTTTLIVTI-------ASHAFALFLAAPTLLASQF 303

Query: 291 ----LRNGGLERVGAQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHRKAMVRLFVERKE 350
               LRN  L +  A+L+ AAKG+YIL+R+LDT SRLV R+ D V+H +AM   +V R  
Sbjct: 304 KPAGLRN-KLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGS 363

Query: 351 DKFAVAVAM-DELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
            +   +  +  ELK+   S  ++++++EEH+Y+C +TINR R L++ ++
Sbjct: 364 GRVRGSEEVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEI 397

BLAST of Lag0029063 vs. TAIR 10
Match: AT1G20180.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 103.6 bits (257), Expect = 3.6e-22
Identity = 97/359 (27.02%), Postives = 174/359 (48.47%), Query Frame = 0

Query: 45  KQQIRNKSQKSFNVNDEYLCSLGTKSYIEFFTKVR--------SIVHESPPSTSSSSTDL 104
           K+  R+ + K  +VN+EY  +  T SY+E  TK          S +  S PS SSSS   
Sbjct: 24  KECCRSLTSK-LSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSSSPSPSSSSDLS 83

Query: 105 RRKFSETILLQPGQLEAIPSILESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANLKS 164
                   LL P Q E + ++++         L  L+  +F++S+EA ++C  LL  L+ 
Sbjct: 84  FHSHFTDYLLDPPQ-ETLDALMQD------SSLDNLIVTFFDLSSEACDVCETLLQCLQQ 143

Query: 165 ARSNSRFIQQSLDSIEK-CSSAEKIETIASDL-------LAQRAPFSD-----IDKRDFA 224
            + N   I++ +   ++ C+ A+ +E     L       L++ A   +     +++  F 
Sbjct: 144 IKINHNKIKRVMKIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFR 203

Query: 225 LIHDEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGLIAITSRTLTALVMAAGPGPAHFG 284
           ++HD  S +  +L   ++++ RKI  + +       L+ + S  L               
Sbjct: 204 IVHDANSDLLTKLTSKKRRIRRKISILGVF--AAPALLGLCSFCL--------------- 263

Query: 285 FRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHRK 344
             L+  + K   H+  ++  LE++G Q++ AAKG +IL  +LDT SRL  RL D ++HRK
Sbjct: 264 --LRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRK 323

Query: 345 AMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
            +  +  + ++ +  +  A+ E          Q++++EEHLY+C  TINR+R LV+ Q+
Sbjct: 324 TVAAMCAKSRKIE-VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 354

BLAST of Lag0029063 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 88.6 bits (218), Expect = 1.2e-17
Identity = 91/366 (24.86%), Postives = 166/366 (45.36%), Query Frame = 0

Query: 35  PVLISDFADGKQQIRNKSQKSFNVNDEYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSST 94
           P L S  A   +   + S  +FN++ E   +  T SY +  ++V  +V  +         
Sbjct: 24  PSLESGVASTSRNDASISSPTFNLSRELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQP 83

Query: 95  DLRRKFSETILLQPGQLEAIPSILESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANL 154
           D+    S+  +LQP +      + E+   +    L  L++ YF  S +A+ LC  L  N+
Sbjct: 84  DIELLLSQ--VLQPNK----ECVQEAIRHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNV 143

Query: 155 KSARSNSRFIQQSLDSIEKCSSAEKIETIASDLLAQ--------RAPFSDIDKRDFALIH 214
            SAR +       L +I    S   I+    DL             PFS  +   F    
Sbjct: 144 HSARHHLYTPLLDLFNIFPGDSLPAIDESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQ 203

Query: 215 DEYSAVSYRLNCSRKKVARKIRSI--KIIDRFTCGLIAITSRTLTALVMAAG-------- 274
             +S + + L+   +K   ++R I         C + A+ +   +A+V+A+         
Sbjct: 204 LCFSQLKHNLDRRLRKSRSRVRLIHHATAGSSLCLVAAVVAVAASAVVIASHALPILLVV 263

Query: 275 PGPAHFGFRLKSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYILNRELDTTSRLVVRLG 334
            GP    +   SF+RK L +             QL  A+KG+++LN++LDT  RLV RL 
Sbjct: 264 AGPLCSPYLPHSFKRKELTNI-----------CQLNAASKGTFVLNKDLDTIDRLVSRLH 323

Query: 335 DAVDHRKAMVRLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRT 383
             +++ K ++RL +ER  D  ++   +  L+KS+L +  Q++D+E+H+ +    +N+ R+
Sbjct: 324 TGIEYDKFLIRLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARS 372

BLAST of Lag0029063 vs. TAIR 10
Match: AT3G19330.3 (Protein of unknown function (DUF677) )

HSP 1 Score: 85.9 bits (211), Expect = 7.7e-17
Identity = 89/356 (25.00%), Postives = 158/356 (44.38%), Query Frame = 0

Query: 35  PVLISDFADGKQQIRNKSQKSFNVNDEYLCSLGTKSYIEFFTKVRSIVHESPPSTSSSST 94
           P L S  A   +   + S  +FN++ E   +  T SY +  ++V  +V  +         
Sbjct: 24  PSLESGVASTSRNDASISSPTFNLSRELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQP 83

Query: 95  DLRRKFSETILLQPGQLEAIPSILESPLLLMMPELKGLLTDYFNVSAEASNLCNRLLANL 154
           D+    S+  +LQP +      + E+   +    L  L++ YF  S +A+ LC  L  N+
Sbjct: 84  DIELLLSQ--VLQPNK----ECVQEAIRHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNV 143

Query: 155 KSARSNSRFIQQSLDSIEKCSSAEKIETIASDLLAQ--------RAPFSDIDKRDFALIH 214
            SAR +       L +I    S   I+    DL             PFS  +   F    
Sbjct: 144 HSARHHLYTPLLDLFNIFPGDSLPAIDESLCDLAFDVFLKLDTFENPFSSPESYSFRDTQ 203

Query: 215 DEYSAVSYRLNCSRKKVARKIRSIKIIDRFTCGLIAITSRTLTALVMAAGPGPAHFGFRL 274
             +S + + L+       R++R                SR+   L+  A  GP    +  
Sbjct: 204 LCFSQLKHNLD-------RRLRK---------------SRSRVRLIHHATAGPLCSPYLP 263

Query: 275 KSFRRKLLRHQMLRNGGLERVGAQLEEAAKGSYILNRELDTTSRLVVRLGDAVDHRKAMV 334
            SF+RK L +             QL  A+KG+++LN++LDT  RLV RL   +++ K ++
Sbjct: 264 HSFKRKELTNI-----------CQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLI 323

Query: 335 RLFVERKEDKFAVAVAMDELKKSNLSIRKQVEDVEEHLYMCIVTINRTRTLVINQM 383
           RL +ER  D  ++   +  L+KS+L +  Q++D+E+H+ +    +N+ R+L++ ++
Sbjct: 324 RLGLERGRDVHSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 340