Lag0021673 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0021673
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionsalutaridinol 7-O-acetyltransferase-like
Locationchr7: 10533125 .. 10534474 (-)
RNA-Seq ExpressionLag0021673
SyntenyLag0021673
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGTTAAAATCTTGTCAAGAGAAACTATTAAGCCTTCTTCCCCAACTCCCCCTCACCTCAAAACCTTTCAACTTTCACTCATCGATCAGTTGTCTCCCATGTTCTATACTCCACTTCTATTTTTCTTCCCTATGAAAAAAAATGACGATGTTCCAAATGACGTCCATCCAAAAAATTTACTTGCTACACTCAAAACCTCCTTATCAGAAACTCTGAGCCGTTTCTTCTTGTTGGCAGGAAGAATCATCGACAGATCCATTCATTGTAGCGACAAAGGAGCTATCTTCCTCGAAGCAAGTGTTAGCTGTAACATGTTGGAGGTCCTCAAAGAACCAAACAACGAGGTCTTAATCAAACTTCTCCCATGTTCTGTCTTTTGTACCGAGCCAGTCGAGAAATACGCTCAAATCGTCGTTCAAGCGAACGTCTTTGACTGCGGTGGAGTCGCAATCTCTCTTTGTCTATTGCACAAGCTCATAGACGTAGCAACTCTCAGCTGTTTTCTAAGATCTTGGGCTTCCACCAACAAAGACTCTCTCAATTCATCTCCAAAGATGTCATGCTCCGACTACAAAGCCTTCTCTTCACTCTTCCCACAAACAAACTCATTGCCTTTCCATCCCCAAGTACTCCATTCGAGTTCCGACCGAAAGCGTCGTCTACGACGGTTCGTGTTTCGTGGGAAAGCGATATCAGATCTAAAAGCCAGAGCCAGATCCAGCAACGTCCCAAATCCGACAAGCGTCGAAGCACTAACTGGGTTTATTTGGAAACATGTAATGAAAGCCTCAACGAGTGGAACTAGCTCTCGAAGTGAAAAGCTGTCGACTTTATCGCACGTAGTGAACTTGAGAAAGAGAATGGAGCCGTCGTTGCCTGAAGTTTCTGTGGGAAACGTCATGTGGGGAGCCATCGCTCATTACAATTCAGCGAACGAATCGATCGAAGTGAAGCTAAGCGAGCTGGTGGGATTATTGAGACAATCGTTTGAAAAAATCAACAGTGATTTCGTAAAAGGGTTGATGGGAGATAAAGGAACGAGAGGCGTTTTGAAGCTGATTGGCGAGATGAATAAGTTTCCATCTGAAGTTCGAGATTACTACTTTTTCACGAGTTGGCGAAACATGGATTTGAATGAAGTTGATTTTGGATGGGGAAAGCCTGCATGGATTGGAATTGCAGGAGACTTGGAAGAGATCATGACGAATATTATTGTTCTTTTGGATACTGTGTCGGGAGATGGAGTGGAGGCTTGGATGGTATTGGATGAAAAGGAAATGAAATTTTTAGAACAGAATCCACAGTTTCTCCAGTTTGCATTGCTGAATCCGAGTGTTGCAATTTAG

mRNA sequence

ATGGAAGTTAAAATCTTGTCAAGAGAAACTATTAAGCCTTCTTCCCCAACTCCCCCTCACCTCAAAACCTTTCAACTTTCACTCATCGATCAGTTGTCTCCCATGTTCTATACTCCACTTCTATTTTTCTTCCCTATGAAAAAAAATGACGATGTTCCAAATGACGTCCATCCAAAAAATTTACTTGCTACACTCAAAACCTCCTTATCAGAAACTCTGAGCCGTTTCTTCTTGTTGGCAGGAAGAATCATCGACAGATCCATTCATTGTAGCGACAAAGGAGCTATCTTCCTCGAAGCAAGTGTTAGCTGTAACATGTTGGAGGTCCTCAAAGAACCAAACAACGAGGTCTTAATCAAACTTCTCCCATGTTCTGTCTTTTGTACCGAGCCAGTCGAGAAATACGCTCAAATCGTCGTTCAAGCGAACGTCTTTGACTGCGGTGGAGTCGCAATCTCTCTTTGTCTATTGCACAAGCTCATAGACGTAGCAACTCTCAGCTGTTTTCTAAGATCTTGGGCTTCCACCAACAAAGACTCTCTCAATTCATCTCCAAAGATGTCATGCTCCGACTACAAAGCCTTCTCTTCACTCTTCCCACAAACAAACTCATTGCCTTTCCATCCCCAAGTACTCCATTCGAGTTCCGACCGAAAGCGTCGTCTACGACGGTTCGTGTTTCGTGGGAAAGCGATATCAGATCTAAAAGCCAGAGCCAGATCCAGCAACGTCCCAAATCCGACAAGCGTCGAAGCACTAACTGGGTTTATTTGGAAACATGTAATGAAAGCCTCAACGAGTGGAACTAGCTCTCGAAGTGAAAAGCTGTCGACTTTATCGCACGTAGTGAACTTGAGAAAGAGAATGGAGCCGTCGTTGCCTGAAGTTTCTGTGGGAAACGTCATGTGGGGAGCCATCGCTCATTACAATTCAGCGAACGAATCGATCGAAGTGAAGCTAAGCGAGCTGGTGGGATTATTGAGACAATCGTTTGAAAAAATCAACAGTGATTTCGTAAAAGGGTTGATGGGAGATAAAGGAACGAGAGGCGTTTTGAAGCTGATTGGCGAGATGAATAAGTTTCCATCTGAAGTTCGAGATTACTACTTTTTCACGAGTTGGCGAAACATGGATTTGAATGAAGTTGATTTTGGATGGGGAAAGCCTGCATGGATTGGAATTGCAGGAGACTTGGAAGAGATCATGACGAATATTATTGTTCTTTTGGATACTGTGTCGGGAGATGGAGTGGAGGCTTGGATGGTATTGGATGAAAAGGAAATGAAATTTTTAGAACAGAATCCACAGTTTCTCCAGTTTGCATTGCTGAATCCGAGTGTTGCAATTTAG

Coding sequence (CDS)

ATGGAAGTTAAAATCTTGTCAAGAGAAACTATTAAGCCTTCTTCCCCAACTCCCCCTCACCTCAAAACCTTTCAACTTTCACTCATCGATCAGTTGTCTCCCATGTTCTATACTCCACTTCTATTTTTCTTCCCTATGAAAAAAAATGACGATGTTCCAAATGACGTCCATCCAAAAAATTTACTTGCTACACTCAAAACCTCCTTATCAGAAACTCTGAGCCGTTTCTTCTTGTTGGCAGGAAGAATCATCGACAGATCCATTCATTGTAGCGACAAAGGAGCTATCTTCCTCGAAGCAAGTGTTAGCTGTAACATGTTGGAGGTCCTCAAAGAACCAAACAACGAGGTCTTAATCAAACTTCTCCCATGTTCTGTCTTTTGTACCGAGCCAGTCGAGAAATACGCTCAAATCGTCGTTCAAGCGAACGTCTTTGACTGCGGTGGAGTCGCAATCTCTCTTTGTCTATTGCACAAGCTCATAGACGTAGCAACTCTCAGCTGTTTTCTAAGATCTTGGGCTTCCACCAACAAAGACTCTCTCAATTCATCTCCAAAGATGTCATGCTCCGACTACAAAGCCTTCTCTTCACTCTTCCCACAAACAAACTCATTGCCTTTCCATCCCCAAGTACTCCATTCGAGTTCCGACCGAAAGCGTCGTCTACGACGGTTCGTGTTTCGTGGGAAAGCGATATCAGATCTAAAAGCCAGAGCCAGATCCAGCAACGTCCCAAATCCGACAAGCGTCGAAGCACTAACTGGGTTTATTTGGAAACATGTAATGAAAGCCTCAACGAGTGGAACTAGCTCTCGAAGTGAAAAGCTGTCGACTTTATCGCACGTAGTGAACTTGAGAAAGAGAATGGAGCCGTCGTTGCCTGAAGTTTCTGTGGGAAACGTCATGTGGGGAGCCATCGCTCATTACAATTCAGCGAACGAATCGATCGAAGTGAAGCTAAGCGAGCTGGTGGGATTATTGAGACAATCGTTTGAAAAAATCAACAGTGATTTCGTAAAAGGGTTGATGGGAGATAAAGGAACGAGAGGCGTTTTGAAGCTGATTGGCGAGATGAATAAGTTTCCATCTGAAGTTCGAGATTACTACTTTTTCACGAGTTGGCGAAACATGGATTTGAATGAAGTTGATTTTGGATGGGGAAAGCCTGCATGGATTGGAATTGCAGGAGACTTGGAAGAGATCATGACGAATATTATTGTTCTTTTGGATACTGTGTCGGGAGATGGAGTGGAGGCTTGGATGGTATTGGATGAAAAGGAAATGAAATTTTTAGAACAGAATCCACAGTTTCTCCAGTTTGCATTGCTGAATCCGAGTGTTGCAATTTAG

Protein sequence

MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKNLLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLIKLLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTNKDSLNSSPKMSCSDYKAFSSLFPQTNSLPFHPQVLHSSSDRKRRLRRFVFRGKAISDLKARARSSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPEVSVGNVMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLMGDKGTRGVLKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWMVLDEKEMKFLEQNPQFLQFALLNPSVAI
Homology
BLAST of Lag0021673 vs. NCBI nr
Match: XP_022138192.1 (BAHD acyltransferase BIA1-like [Momordica charantia])

HSP 1 Score: 610.5 bits (1573), Expect = 1.1e-170
Identity = 308/459 (67.10%), Postives = 372/459 (81.05%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           MEVKILSRETI PSSPTPPHLKTF+LSLIDQLSP  Y PLLFF+P+K   D+    + K 
Sbjct: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETH-NQKK 60

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDRSIHCS-DKGAIFLEASVSCNMLEVLKEPNNEVLI 120
            +ATLK SLS+TL+ FFLLAGRI + SI CS D+GA+F+EA+V+CN+ ++LKEPNNEVL 
Sbjct: 61  AIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLT 120

Query: 121 KLLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTNKD 180
           KLLPCS+ CT+P E+YAQIV QAN+F+CGG+ ISLCLLHKL+D  + SCFL+SWAS NK 
Sbjct: 121 KLLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKG 180

Query: 181 SL-NSSPKMSCSDYKAFSSLFPQTNSLPFHPQV------LHSSSDRKRRLRRFVFRGKAI 240
           SL NSS  + CS+YK FSSLFPQTN L FH Q+      + SSS   R L+RFVF  KAI
Sbjct: 181 SLDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAI 240

Query: 241 SDLKARARSSNVPNPTSVEALTGFIWKHVM--KASTSGTSSRSEKLSTLSHVVNLRKRME 300
           SDLKA+A+S+ VPNPTSVEALTGFIW   M  KA++ G    S++ STLSHVVNLRKRME
Sbjct: 241 SDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRME 300

Query: 301 PSLPEVSVGNVMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLMGDKGTRG 360
           P LPE+S+GNV+WG +AHY S+N   +V+L +LV LLRQSF KIN+DF+KGLMG+ G R 
Sbjct: 301 PPLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRA 360

Query: 361 VLKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLD 420
           VL+L+GE+NKFPS+V +YYFFTSWRNMDLNEVDFGWGKP WIGIAG+   I  + I+LLD
Sbjct: 361 VLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLD 420

Query: 421 TVSGDGVEAWMVLDEKEMKFLEQNPQFLQFALLNPSVAI 450
           T SGDG+EAWMVLD+K+MK L+QNPQFL+FA LNPS++I
Sbjct: 421 TPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISI 458

BLAST of Lag0021673 vs. NCBI nr
Match: XP_004152093.1 (stemmadenine O-acetyltransferase [Cucumis sativus])

HSP 1 Score: 589.3 bits (1518), Expect = 2.7e-164
Identity = 306/472 (64.83%), Postives = 382/472 (80.93%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDD--VPNDVHP 60
           MEVK+LS+ETI PSSPTPPHL+TFQLSL+DQLSPM Y PLL F+PMK++ D    +D  P
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60

Query: 61  KNLLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVL 120
           K  +ATLKTSLS+TLSRF+LLAGRIID+SIHC+DKGA+F+EA+++ NM ++LKEPNNEVL
Sbjct: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120

Query: 121 IKLLPCSVFC-TEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTN 180
            KLLPCS+ C T+P+E+Y QIVVQANVF CGG+AISLCLLHKLID AT  CFLRSWA+TN
Sbjct: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180

Query: 181 KDSL----NSSP-KMSCSDYKAFSSLFPQTNSLPFHPQVLHSSS---------DRKRRLR 240
           ++ L    +SSP  M C DYK+FSSLFPQTN LPFH +++++ +         + KRRL+
Sbjct: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRLINNDNAVILPSSIFNGKRRLQ 240

Query: 241 RFVFRGKAISDLKARARSSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVV 300
           RFVFR KAI DLKA+A+S ++PNPT VEA+T FIWK++MK +  G    S+  STLSHVV
Sbjct: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGD---SQMPSTLSHVV 300

Query: 301 NLRKRMEPSLPEVSVGNVMWGAIAHYNSA---NESIE-VKLSELVGLLRQSFEKINSDFV 360
           N+RK +EPSL EVS+GN+MWG +AH+ S    NES E ++LS+LV LLRQS +KIN D++
Sbjct: 301 NIRKMIEPSLGEVSLGNIMWGTVAHHFSTTRPNESFEGLELSKLVSLLRQSLKKINKDYI 360

Query: 361 KGLM--GDKGTR-GVLKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAG 420
           K L+  GDKG R GV+KL+GE+NK+P  + +YYFFTSW+NM LNE+DFGWGKP W GIAG
Sbjct: 361 KELIMGGDKGRRNGVMKLVGEINKWP--ISNYYFFTSWKNMKLNELDFGWGKPLWFGIAG 420

Query: 421 DLEEIMTNIIVLLDTVSGDG-VEAWMVLDEKEMKFLEQNPQFLQFALLNPSV 448
           D  E+M NIIVL+D VS DG +EAW++LDEKEM+ LEQNPQFL+FALLNPS+
Sbjct: 421 DSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSI 467

BLAST of Lag0021673 vs. NCBI nr
Match: KAE8649121.1 (hypothetical protein Csa_015082 [Cucumis sativus])

HSP 1 Score: 587.0 bits (1512), Expect = 1.3e-163
Identity = 305/474 (64.35%), Postives = 381/474 (80.38%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDD--VPNDVHP 60
           MEVK+LS+ETI PSSPTPPHL+TFQLSL+DQLSPM Y PLL F+PMK++ D    +D  P
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60

Query: 61  KNLLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVL 120
           K  +ATLKTSLS+TLSRF+LLAGRIID+SIHC+DKGA+F+EA+++ NM ++LKEPNNEVL
Sbjct: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120

Query: 121 IKLLPCSVFC-TEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTN 180
            KLLPCS+ C T+P+E+Y QIVVQANVF CGG+AISLCLLHKLID AT  CFLRSWA+TN
Sbjct: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180

Query: 181 KDSL----NSSP-KMSCSDYKAFSSLFPQTNSLPFHPQVLHSSS---------DRKRRLR 240
           ++ L    +SSP  M C DYK+FSSLFPQTN LPFH +++++ +         + KRRL+
Sbjct: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRLINNDNAVILPSSIFNGKRRLQ 240

Query: 241 RFVFRGKAISDLKARARSSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVV 300
           RFVFR KAI DLKA+A+S ++PNPT VEA+T FIWK++MK +  G    S+  STLSHVV
Sbjct: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGD---SQMPSTLSHVV 300

Query: 301 NLRKRMEPSLPEVSVGNVMWGAIAHYNSA---NESIE-VKLSELVGLLRQSFEKINSDFV 360
           N+RK +EPSL EVS+GN+MWG +AH+ S    NES E ++LS+LV LLRQS +KIN D++
Sbjct: 301 NIRKMIEPSLGEVSLGNIMWGTVAHHFSTTRPNESFEGLELSKLVSLLRQSLKKINKDYI 360

Query: 361 KGLM--GDKGTR-GVLKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAG 420
           K L+  GDKG R GV+KL+GE+NK+P  + +YYFFTSW+NM LNE+DFGWGKP W GIAG
Sbjct: 361 KELIMGGDKGRRNGVMKLVGEINKWP--ISNYYFFTSWKNMKLNELDFGWGKPLWFGIAG 420

Query: 421 DLEEIMTNIIVLLDTVSGDG-VEAWMVLDEKEMKFLEQNPQFLQFALLNPSVAI 450
           D  E+M NIIVL+D VS DG +EAW++LDEKEM+ LEQNPQFL+FALLNP   +
Sbjct: 421 DSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPRFCV 469

BLAST of Lag0021673 vs. NCBI nr
Match: KAG6582063.1 (Salutaridinol 7-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 580.9 bits (1496), Expect = 9.7e-162
Identity = 299/449 (66.59%), Postives = 359/449 (79.96%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           MEVK+LSRETI PSSPTPPHLK+F+LSL+DQLSPM YTPLLFF+P+ K  D      P  
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLKSFKLSLLDQLSPMLYTPLLFFYPITKTYD------PNP 60

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLIK 120
            +ATLK SLS+TL+RF+LLAGRI+ +SIHCSD+GA+F+EASVSCNM  +LK+PNNEVL K
Sbjct: 61  TMATLKDSLSQTLTRFYLLAGRIVGKSIHCSDQGAVFIEASVSCNMSHILKQPNNEVLRK 120

Query: 121 LLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTNKDS 180
           LLPCS+ C+ P E+  Q+VVQAN+F CGG+AISLCLLHKL+D AT + FL SWASTNK S
Sbjct: 121 LLPCSLLCSNPAEESPQVVVQANIFSCGGIAISLCLLHKLMDGATFTSFLTSWASTNKGS 180

Query: 181 LNSSPKMSCSDYKAFSSLFPQTNSLPFHPQVLHSSSDRKRRLRRFVFRGKAISDLKARAR 240
           L+SS  M C DYKAFSSLFPQTN LPFH Q L   ++RKR LRRFVF  K++ +LKA+A 
Sbjct: 181 LDSSSNMICYDYKAFSSLFPQTNLLPFH-QKLAKPANRKRCLRRFVFGTKSLLNLKAKAE 240

Query: 241 SSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPEVSVGN 300
           S  VPNP+ VEALTGFIWKH M A+    S      STLSHVVNLR RM+P+LPEVSVGN
Sbjct: 241 SRAVPNPSCVEALTGFIWKHAMMAADCQGS------STLSHVVNLRGRMKPALPEVSVGN 300

Query: 301 VMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLM--GDKGTRGVLKLIGEM 360
           +MWGA+AH +SA E +E  L  LVGLLRQSFE+IN D+++ ++   DKG   VLK +GEM
Sbjct: 301 IMWGAVAH-SSAMERLE--LRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVGEM 360

Query: 361 NKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVE 420
           NK+   + +YYF TSWRNM LNEVDFGWG+PAWI IAGD  E+M N+IVL+D V GDG+E
Sbjct: 361 NKW--GMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAGDTNELMANVIVLVDGVMGDGIE 420

Query: 421 AWMVLDEKEMKFLEQNPQFLQFALLNPSV 448
           AW++LDEKEM+ LEQNPQFL+FALLNPS+
Sbjct: 421 AWILLDEKEMEILEQNPQFLEFALLNPSI 431

BLAST of Lag0021673 vs. NCBI nr
Match: XP_022956252.1 (salutaridinol 7-O-acetyltransferase-like [Cucurbita moschata])

HSP 1 Score: 580.5 bits (1495), Expect = 1.3e-161
Identity = 299/449 (66.59%), Postives = 358/449 (79.73%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           MEVK+LSRETI PSSPTPPHLKTF+LSL+DQLSPM YTPLLFF+P+ K  D      P  
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLKTFELSLLDQLSPMLYTPLLFFYPITKTYD------PNP 60

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLIK 120
            +ATLK SLS+TL+RF+LLAGRI+ +SIHCSD+GA+F+EASVSCNM  +LK+PNNEVL K
Sbjct: 61  TMATLKDSLSQTLTRFYLLAGRIVGKSIHCSDQGAVFIEASVSCNMSHILKQPNNEVLRK 120

Query: 121 LLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTNKDS 180
           LLPCS+ C+ P E+  Q+VVQAN+F CGG+AISLCLLHKL+D AT + FL SWASTNK S
Sbjct: 121 LLPCSLLCSNPAEESPQVVVQANIFSCGGIAISLCLLHKLMDGATFTSFLTSWASTNKGS 180

Query: 181 LNSSPKMSCSDYKAFSSLFPQTNSLPFHPQVLHSSSDRKRRLRRFVFRGKAISDLKARAR 240
           L+SS  M C DYKAFSSLFPQTN LPFH Q L   ++RKR LRRFVF  K++ +LKA+A 
Sbjct: 181 LDSSSNMICYDYKAFSSLFPQTNLLPFH-QKLAKPANRKRCLRRFVFGSKSLLNLKAKAE 240

Query: 241 SSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPEVSVGN 300
           S  VPNP+ VEALTGFIWKH M A+    S      STLSHVVNLR RM+P+LPEVSVGN
Sbjct: 241 SRAVPNPSCVEALTGFIWKHAMMAADCQGS------STLSHVVNLRGRMKPALPEVSVGN 300

Query: 301 VMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLM--GDKGTRGVLKLIGEM 360
           +MWGA+AH +SA E +E  L  LVGLLRQSFE+IN D+++ ++   DKG   VLK +GEM
Sbjct: 301 IMWGAVAH-SSAMERLE--LRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVGEM 360

Query: 361 NKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVE 420
           NK+   + +YYF TSWRNM LNEVDFGWG+PAWI IA D  E+M N+IVL+D V GDG+E
Sbjct: 361 NKW--GMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNELMANVIVLVDGVMGDGIE 420

Query: 421 AWMVLDEKEMKFLEQNPQFLQFALLNPSV 448
           AW++LDEKEM+ LEQNPQFL+FALLNPS+
Sbjct: 421 AWILLDEKEMEILEQNPQFLEFALLNPSI 431

BLAST of Lag0021673 vs. ExPASy Swiss-Prot
Match: Q94FT4 (Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 3.3e-56
Identity = 156/468 (33.33%), Postives = 244/468 (52.14%), Query Frame = 0

Query: 3   VKILSRETIKPSSPTPPHLKTFQLSLIDQLSPM-FYTPLLFFFPMKKNDDVPNDVHPKNL 62
           V+++S+ETIKP++PTP  LK F LSL+DQ  P+ +Y P++ F+P    +   +  H  + 
Sbjct: 9   VEVISKETIKPTTPTPSQLKNFNLSLLDQCFPLYYYVPIILFYPATAANSTGSSNHHDD- 68

Query: 63  LATLKTSLSETLSRFFLLAGRIIDR-SIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLIK 122
           L  LK+SLS+TL  F+ +AGR+ID   + C D+G  F +  +   M E + +P +  L +
Sbjct: 69  LDLLKSSLSKTLVHFYPMAGRMIDNILVDCHDQGINFYKVKIRGKMCEFMSQP-DVPLSQ 128

Query: 123 LLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTNKDS 182
           LLP  V  +  V K A ++VQ N+FDCGG AI   + HK+ D AT+S F+RSWAST K S
Sbjct: 129 LLPSEV-VSASVPKEALVIVQVNMFDCGGTAICSSVSHKIADAATMSTFIRSWASTTKTS 188

Query: 183 LNSSPKMSCSDYKAF-----SSLFPQT---------NSLPFHPQVLHSSSDRKRRLRRFV 242
            +     + +D K       +SLFP +         + +PF       + D K   +RFV
Sbjct: 189 RSGGSTAAVTDQKLIPSFDSASLFPPSERLTSPSGMSEIPF-SSTPEDTEDDKTVSKRFV 248

Query: 243 FRGKAISDLKARARSSNVPN-----PTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSH 302
           F    I+ ++ + +     N      T VE +T  IWK VMK++ +G       L  + H
Sbjct: 249 FDFAKITSVREKLQVLMHDNYKSRRQTRVEVVTSLIWKSVMKSTPAGF------LPVVHH 308

Query: 303 VVNLRKRMEPSLPEVSVGNVMWGAIAHY--------NSANESIEVKLSELVGLLRQSFE- 362
            VNLRK+M+P L +VS GN+     A          N+ N++I    SE   +L +  + 
Sbjct: 309 AVNLRKKMDPPLQDVSFGNLSVTVSAFLPATTTTTTNAVNKTINSTSSESQVVLHELHDF 368

Query: 363 --KINSDFVKGLMGDKGT--RGVLKLIGEMNKFPSEVRDY----YFFTSWRNMDLNEVDF 422
             ++ S+  K + GDKG+  + +       +    ++ D     ++ +SW  M L E+DF
Sbjct: 369 IAQMRSEIDK-VKGDKGSLEKVIQNFASGHDASIKKINDVEVINFWISSWCRMGLYEIDF 428

Query: 423 GWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWMVLDEKEMKFLE 433
           GWGKP W+ +  +++    N   + DT  G+G+E W    E +M   E
Sbjct: 429 GWGKPIWVTVDPNIKP-NKNCFFMNDTKCGEGIEVWASFLEDDMAKFE 464

BLAST of Lag0021673 vs. ExPASy Swiss-Prot
Match: I3PLR4 ((13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver somniferum OX=3469 GN=AT1 PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.1e-51
Identity = 149/469 (31.77%), Postives = 236/469 (50.32%), Query Frame = 0

Query: 3   VKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKNLL 62
           V+++S+ETIKP +PTP  L+ + +SL+DQ S + Y P++ F+P   + +     H  + L
Sbjct: 9   VEVISKETIKPRNPTPYQLRNYNMSLLDQYSSLVYVPIILFYPAASDANSTGSKHHDD-L 68

Query: 63  ATLKTSLSETLSRFFLLAGRIIDR-SIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLIKL 122
             LK SLSETL  F+ +AGR+ D  ++ C+D+G  F E  +   M + + + +  + + L
Sbjct: 69  HLLKRSLSETLVHFYPMAGRMKDNMTVDCNDEGIDFFEVRIKGRMCDFMMKSDAHLSL-L 128

Query: 123 LPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWAST----N 182
           LP  V  T  V K AQ++VQ N+FDCGG AI  C+ +K+ D  T+  F+RS A T     
Sbjct: 129 LPSEVASTNFV-KEAQVIVQVNMFDCGGTAICFCISNKIADACTMITFIRSLAGTTNIAR 188

Query: 183 KDSLNSSPKMSCSDYKAF--SSLFPQTNSLPFHPQVLHSSSDR----KRRLRRFVFRGKA 242
           + S  ++P  + +   +F  +SLFP +  L    QV + + D     K   +RFVF    
Sbjct: 189 RGSSIAAPTTNQNLVPSFDSTSLFPPSEQLA--SQVSYPTQDSTSVDKLVSKRFVFDAAK 248

Query: 243 ISDLKARARS-----SNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLR 302
           I+  + + +S          PT VE ++  IWK  +K++  G+      +ST++H +N R
Sbjct: 249 ITSAREKLQSLMHDKYKCHRPTRVEVVSALIWKSAVKSAPPGS------ISTVTHAMNFR 308

Query: 303 KRMEPSLPEVSVGNVMW---------------GAIAHYNSANESIEVKLSEL---VGLLR 362
           K+M+P L + S GN+                  A    +S +   +V L EL   V LLR
Sbjct: 309 KKMDPPLQDASFGNLCVVVTAVLPATTATTTNPATKKVSSTSNEEQVALDELSDFVALLR 368

Query: 363 QSFEKINSDFVKGLMGDKGTRGVLKLIGEMNKFPSEVRD---YYFFTSWRNMDLNEVDFG 422
           +  +K+  D  KG M     + +      + K  S+V D     F TSW      E DFG
Sbjct: 369 REIDKVKGD--KGCMEKIIQKFIYGHDASVAK-DSDVEDKVTALFMTSWCKFGFYEADFG 428

Query: 423 WGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVEAWMVLDEKEMKFLEQN 435
           WG P W+     +E    N++ + D   G+G+E W+   E +M   E +
Sbjct: 429 WGTPVWVTTVPLIEPKYKNMVFMNDMKCGEGIEVWVNFLEDDMTKFEHH 463

BLAST of Lag0021673 vs. ExPASy Swiss-Prot
Match: O23393 (BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.9e-51
Identity = 149/455 (32.75%), Postives = 244/455 (53.63%), Query Frame = 0

Query: 2   EVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKNL 61
           ++++  +E IKP+SP+P      QLS++D   P  Y   +FF+ +       ++V  +N 
Sbjct: 4   KLEVTGKEVIKPASPSP--RDRLQLSILDLYCPGIYVSTIFFYDLITES---SEVFSEN- 63

Query: 62  LATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLIKL 121
              LK SLSETLSRF+ LAGRI   SI C+D+GA+F EA     + + L+  N + L   
Sbjct: 64  ---LKLSLSETLSRFYPLAGRIEGLSISCNDEGAVFTEARTDLLLPDFLRNLNTDSLSGF 123

Query: 122 LPCSVFCTEPVEKYAQIVVQANVFDCG-GVAISLCLLHKLIDVATLSCFLRSWASTN-KD 181
           LP ++   E    +  + V+   F  G GVA+S+ + HK+ D+A+L  F++ WA+T  K 
Sbjct: 124 LP-TLAAGESPAAWPLLSVKVTFFGSGSGVAVSVSVSHKICDIASLVTFVKDWATTTAKG 183

Query: 182 SLNSSPKMSCSDYKAFSSLFPQTNSLPFHPQVLHSSSD----RKRRLRRFVFRGKAISDL 241
             NS+ +       A ++++P   S   + Q   + SD     K  L+RFVF    I++L
Sbjct: 184 KSNSTIEF------AETTIYPPPPS-HMYEQFPSTDSDSNITSKYVLKRFVFEPSKIAEL 243

Query: 242 KARARSSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSL-P 301
           K +A S +VP PT VEA+   IW+    +S S  +    + + +   +++R R+  S+ P
Sbjct: 244 KHKAASESVPVPTRVEAIMSLIWRCARNSSRS--NLLIPRQAVMWQAMDIRLRIPSSVAP 303

Query: 302 EVSVGNVMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLMGDKGTRGVLKL 361
           +  +GN+  G     ++ +   E ++ E+V   R++ E++N    + L G+   + +L L
Sbjct: 304 KDVIGNLQSGFSLKKDAES---EFEIPEIVATFRKNKERVNEMIKESLQGNTIGQSLLSL 363

Query: 362 IGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNI--IVLLDTV 421
           + E     +E+ D Y  +SW      EVDFG G P W+G A     I  N+  +VL+D+ 
Sbjct: 364 MAETVSESTEI-DRYIMSSWCRKPFYEVDFGSGSPVWVGYAS--HTIYDNMVGVVLIDSK 423

Query: 422 SGDGVEAWMVLDEKEMKFLEQNPQFLQFALLNPSV 448
            GDGVEAW+ L E++M     + + L +A+LNP V
Sbjct: 424 EGDGVEAWISLPEEDMSVFVDDQELLAYAVLNPPV 433

BLAST of Lag0021673 vs. ExPASy Swiss-Prot
Match: A0A2P1GIW7 (Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.2e-51
Identity = 143/439 (32.57%), Postives = 236/439 (53.76%), Query Frame = 0

Query: 2   EVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKNL 61
           +++ILS E I+PSSPTP  LKT +LS +DQ+    + P++ F+P + + ++      +N 
Sbjct: 4   QMQILSEELIQPSSPTPQTLKTHKLSHLDQVLLTCHIPIILFYPNQLDSNLDRAQRSEN- 63

Query: 62  LATLKTSLSETLSRFFLLAGRI-IDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLIK 121
              LK SLS  L++F+ LAGRI I+ S+ C+D G  FLEA V   + E +K    + L +
Sbjct: 64  ---LKRSLSTVLTQFYPLAGRININSSVDCNDSGVPFLEARVHSQLSEAIKNVAIDELNQ 123

Query: 122 LLPCSVFC---TEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTN 181
            LP   +       ++K   + V+ + F+CGG AI +C+ HK+ D  +L+ FL SW +T 
Sbjct: 124 YLPFQPYPGGEESGLKKDIPLAVKISCFECGGTAIGVCISHKIADALSLATFLNSWTATC 183

Query: 182 KDSLNSSPKMSCSDYKAFSSLFPQTNSLPFHPQVLHSSSDRKRRLRRFVFRGKAISDLKA 241
           ++  +    +   ++   S  FP   S+P  P+ L    D    ++RFVF  + +  LKA
Sbjct: 184 QEETD----IVQPNFDLGSHHFPPMESIP-APEFL---PDENIVMKRFVFDKEKLEALKA 243

Query: 242 RARSS--NVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPE 301
           +  SS   V N + V+ +   IWK  +  + +   ++++ ++  +  VNLR RM P  P+
Sbjct: 244 QLASSATEVKNSSRVQIVIAVIWKQFIDVTRAKFDTKNKLVA--AQAVNLRSRMNPPFPQ 303

Query: 302 VSVGNVMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLMGDKGTRGVLKLI 361
            ++GN+   A   Y  A E  +   S+LVG L+ S  KI+ + VK L      +GV  L 
Sbjct: 304 SAMGNI---ATMAYAVAEE--DKDFSDLVGPLKTSLAKIDDEHVKELQ-----KGVTYL- 363

Query: 362 GEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGD 421
                + +E ++ + F+SW  +   ++DFGWGKP  +         M N++ L+DT + D
Sbjct: 364 ----DYEAEPQELFSFSSWCRLGFYDLDFGWGKPVSVCTT---TVPMKNLVYLMDTRNED 410

Query: 422 GVEAWMVLDEKEMKFLEQN 435
           G+EAW+ + E EM  L  +
Sbjct: 424 GMEAWISMAEDEMSMLSSD 410

BLAST of Lag0021673 vs. ExPASy Swiss-Prot
Match: Q70PR7 (Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 1.8e-46
Identity = 136/435 (31.26%), Postives = 227/435 (52.18%), Query Frame = 0

Query: 2   EVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKNL 61
           +++ +S E I PSSPTP  LK +++S +DQL    + P + F+P    + + +++ P   
Sbjct: 4   QMEKVSEELILPSSPTPQSLKCYKISHLDQLLLTCHIPFILFYP----NPLDSNLDPAQT 63

Query: 62  LATLKTSLSETLSRFFLLAGRI-IDRSIHCSDKGAIFLEASVSCNMLEVLKE-PNNEVLI 121
              LK SLS+ L+ F+ LAGRI ++ S+ C+D G  F+EA V   + + ++     E L 
Sbjct: 64  SQHLKQSLSKVLTHFYPLAGRINVNSSVDCNDSGVPFVEARVQAQLSQAIQNVVELEKLD 123

Query: 122 KLLPCSVFCTEPVE--KYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTN 181
           + LP + +    +E  +   + V+ + F+CGG AI + L HK+ DV +L+ FL +W +T 
Sbjct: 124 QYLPSAAYPGGKIEVNEDVPLAVKISFFECGGTAIGVNLSHKIADVLSLATFLNAWTAT- 183

Query: 182 KDSLNSSPKMSCSDYKAFSSLFPQTNSLPFHPQVLHSSSDRKRRLRRFVFRGKAISDLKA 241
                   ++   ++   +  FP  ++ P  P+++    D    ++RFVF  + I  L+A
Sbjct: 184 ---CRGETEIVLPNFDLAARHFPPVDNTP-SPELV---PDENVVMKRFVFDKEKIGALRA 243

Query: 242 RARS-SNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPEV 301
           +A S S   N + V+ +  +IWKHV+  + +   ++++    +   VNLR RM P LP  
Sbjct: 244 QASSASEEKNFSRVQLVVAYIWKHVIDVTRAKYGAKNK--FVVVQAVNLRSRMNPPLPHY 303

Query: 302 SVGNVMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLMGDKGTRGVLKLIG 361
           ++GN+   A   + + +   +    +L+G LR S EK   D    L+  KG   + +L  
Sbjct: 304 AMGNI---ATLLFAAVDAEWDKDFPDLIGPLRTSLEKTEDDHNHELL--KGMTCLYEL-- 363

Query: 362 EMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDG 421
                  E ++   FTSW  +   ++DFGWGKP     A        N  +L+DT SGDG
Sbjct: 364 -------EPQELLSFTSWCRLGFYDLDFGWGKPL---SACTTTFPKRNAALLMDTRSGDG 407

Query: 422 VEAWMVLDEKEMKFL 432
           VEAW+ + E EM  L
Sbjct: 424 VEAWLPMAEDEMAML 407

BLAST of Lag0021673 vs. ExPASy TrEMBL
Match: A0A6J1CAD9 (BAHD acyltransferase BIA1-like OS=Momordica charantia OX=3673 GN=LOC111009425 PE=3 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 5.5e-171
Identity = 308/459 (67.10%), Postives = 372/459 (81.05%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           MEVKILSRETI PSSPTPPHLKTF+LSLIDQLSP  Y PLLFF+P+K   D+    + K 
Sbjct: 1   MEVKILSRETIMPSSPTPPHLKTFELSLIDQLSPPLYIPLLFFYPIKNKHDLETH-NQKK 60

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDRSIHCS-DKGAIFLEASVSCNMLEVLKEPNNEVLI 120
            +ATLK SLS+TL+ FFLLAGRI + SI CS D+GA+F+EA+V+CN+ ++LKEPNNEVL 
Sbjct: 61  AIATLKNSLSKTLTNFFLLAGRITNTSIRCSNDEGAVFIEATVNCNISDILKEPNNEVLT 120

Query: 121 KLLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTNKD 180
           KLLPCS+ CT+P E+YAQIV QAN+F+CGG+ ISLCLLHKL+D  + SCFL+SWAS NK 
Sbjct: 121 KLLPCSIQCTKPTEEYAQIVAQANIFNCGGIVISLCLLHKLMDGISFSCFLKSWASINKG 180

Query: 181 SL-NSSPKMSCSDYKAFSSLFPQTNSLPFHPQV------LHSSSDRKRRLRRFVFRGKAI 240
           SL NSS  + CS+YK FSSLFPQTN L FH Q+      + SSS   R L+RFVF  KAI
Sbjct: 181 SLDNSSQNLVCSNYKTFSSLFPQTNLLSFHHQIHEGVDDICSSSKPNRCLQRFVFGAKAI 240

Query: 241 SDLKARARSSNVPNPTSVEALTGFIWKHVM--KASTSGTSSRSEKLSTLSHVVNLRKRME 300
           SDLKA+A+S+ VPNPTSVEALTGFIW   M  KA++ G    S++ STLSHVVNLRKRME
Sbjct: 241 SDLKAKAKSTEVPNPTSVEALTGFIWNCAMKLKATSMGNDDDSQRPSTLSHVVNLRKRME 300

Query: 301 PSLPEVSVGNVMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLMGDKGTRG 360
           P LPE+S+GNV+WG +AHY S+N   +V+L +LV LLRQSF KIN+DF+KGLMG+ G R 
Sbjct: 301 PPLPEISIGNVLWGMVAHYCSSNYETQVELRDLVVLLRQSFSKINNDFIKGLMGNDGNRA 360

Query: 361 VLKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLD 420
           VL+L+GE+NKFPS+V +YYFFTSWRNMDLNEVDFGWGKP WIGIAG+   I  + I+LLD
Sbjct: 361 VLELLGEINKFPSKVPNYYFFTSWRNMDLNEVDFGWGKPIWIGIAGNSNVITKDFILLLD 420

Query: 421 TVSGDGVEAWMVLDEKEMKFLEQNPQFLQFALLNPSVAI 450
           T SGDG+EAWMVLD+K+MK L+QNPQFL+FA LNPS++I
Sbjct: 421 TPSGDGIEAWMVLDQKKMKLLQQNPQFLEFASLNPSISI 458

BLAST of Lag0021673 vs. ExPASy TrEMBL
Match: A0A0A0KXH2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016510 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 1.3e-164
Identity = 306/472 (64.83%), Postives = 382/472 (80.93%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDD--VPNDVHP 60
           MEVK+LS+ETI PSSPTPPHL+TFQLSL+DQLSPM Y PLL F+PMK++ D    +D  P
Sbjct: 1   MEVKVLSKETIIPSSPTPPHLQTFQLSLLDQLSPMLYIPLLLFYPMKRSYDHIDQHDQDP 60

Query: 61  KNLLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVL 120
           K  +ATLKTSLS+TLSRF+LLAGRIID+SIHC+DKGA+F+EA+++ NM ++LKEPNNEVL
Sbjct: 61  KKTIATLKTSLSKTLSRFYLLAGRIIDKSIHCNDKGAVFMEATINTNMFDILKEPNNEVL 120

Query: 121 IKLLPCSVFC-TEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTN 180
            KLLPCS+ C T+P+E+Y QIVVQANVF CGG+AISLCLLHKLID AT  CFLRSWA+TN
Sbjct: 121 TKLLPCSLLCNTKPIEEYPQIVVQANVFKCGGIAISLCLLHKLIDAATFCCFLRSWATTN 180

Query: 181 KDSL----NSSP-KMSCSDYKAFSSLFPQTNSLPFHPQVLHSSS---------DRKRRLR 240
           ++ L    +SSP  M C DYK+FSSLFPQTN LPFH +++++ +         + KRRL+
Sbjct: 181 RELLSQLDHSSPNNMVCFDYKSFSSLFPQTNLLPFHQRLINNDNAVILPSSIFNGKRRLQ 240

Query: 241 RFVFRGKAISDLKARARSSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVV 300
           RFVFR KAI DLKA+A+S ++PNPT VEA+T FIWK++MK +  G    S+  STLSHVV
Sbjct: 241 RFVFRSKAILDLKAKAKSCDIPNPTCVEAITCFIWKYLMKVADGGD---SQMPSTLSHVV 300

Query: 301 NLRKRMEPSLPEVSVGNVMWGAIAHYNSA---NESIE-VKLSELVGLLRQSFEKINSDFV 360
           N+RK +EPSL EVS+GN+MWG +AH+ S    NES E ++LS+LV LLRQS +KIN D++
Sbjct: 301 NIRKMIEPSLGEVSLGNIMWGTVAHHFSTTRPNESFEGLELSKLVSLLRQSLKKINKDYI 360

Query: 361 KGLM--GDKGTR-GVLKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAG 420
           K L+  GDKG R GV+KL+GE+NK+P  + +YYFFTSW+NM LNE+DFGWGKP W GIAG
Sbjct: 361 KELIMGGDKGRRNGVMKLVGEINKWP--ISNYYFFTSWKNMKLNELDFGWGKPLWFGIAG 420

Query: 421 DLEEIMTNIIVLLDTVSGDG-VEAWMVLDEKEMKFLEQNPQFLQFALLNPSV 448
           D  E+M NIIVL+D VS DG +EAW++LDEKEM+ LEQNPQFL+FALLNPS+
Sbjct: 421 DSNEMMGNIIVLVDNVSDDGSIEAWILLDEKEMQLLEQNPQFLEFALLNPSI 467

BLAST of Lag0021673 vs. ExPASy TrEMBL
Match: A0A6J1GVT5 (salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111458004 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 6.1e-162
Identity = 299/449 (66.59%), Postives = 358/449 (79.73%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           MEVK+LSRETI PSSPTPPHLKTF+LSL+DQLSPM YTPLLFF+P+ K  D      P  
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLKTFELSLLDQLSPMLYTPLLFFYPITKTYD------PNP 60

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLIK 120
            +ATLK SLS+TL+RF+LLAGRI+ +SIHCSD+GA+F+EASVSCNM  +LK+PNNEVL K
Sbjct: 61  TMATLKDSLSQTLTRFYLLAGRIVGKSIHCSDQGAVFIEASVSCNMSHILKQPNNEVLRK 120

Query: 121 LLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTNKDS 180
           LLPCS+ C+ P E+  Q+VVQAN+F CGG+AISLCLLHKL+D AT + FL SWASTNK S
Sbjct: 121 LLPCSLLCSNPAEESPQVVVQANIFSCGGIAISLCLLHKLMDGATFTSFLTSWASTNKGS 180

Query: 181 LNSSPKMSCSDYKAFSSLFPQTNSLPFHPQVLHSSSDRKRRLRRFVFRGKAISDLKARAR 240
           L+SS  M C DYKAFSSLFPQTN LPFH Q L   ++RKR LRRFVF  K++ +LKA+A 
Sbjct: 181 LDSSSNMICYDYKAFSSLFPQTNLLPFH-QKLAKPANRKRCLRRFVFGSKSLLNLKAKAE 240

Query: 241 SSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPEVSVGN 300
           S  VPNP+ VEALTGFIWKH M A+    S      STLSHVVNLR RM+P+LPEVSVGN
Sbjct: 241 SRAVPNPSCVEALTGFIWKHAMMAADCQGS------STLSHVVNLRGRMKPALPEVSVGN 300

Query: 301 VMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLM--GDKGTRGVLKLIGEM 360
           +MWGA+AH +SA E +E  L  LVGLLRQSFE+IN D+++ ++   DKG   VLK +GEM
Sbjct: 301 IMWGAVAH-SSAMERLE--LRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVGEM 360

Query: 361 NKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVE 420
           NK+   + +YYF TSWRNM LNEVDFGWG+PAWI IA D  E+M N+IVL+D V GDG+E
Sbjct: 361 NKW--GMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNELMANVIVLVDGVMGDGIE 420

Query: 421 AWMVLDEKEMKFLEQNPQFLQFALLNPSV 448
           AW++LDEKEM+ LEQNPQFL+FALLNPS+
Sbjct: 421 AWILLDEKEMEILEQNPQFLEFALLNPSI 431

BLAST of Lag0021673 vs. ExPASy TrEMBL
Match: A0A6J1GVI6 (salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111457861 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 2.3e-161
Identity = 298/449 (66.37%), Postives = 358/449 (79.73%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           MEVK+LSRETI PSSPTPPHLK+F+LSL+DQLSPM YTPLLFF+P+ K  D      P  
Sbjct: 1   MEVKVLSRETIIPSSPTPPHLKSFKLSLLDQLSPMLYTPLLFFYPITKTYD------PNP 60

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLIK 120
            +ATLK SLS+TL+RF+LLAGRI+ +SIHCSD+GA+F+EASVSCNM  +LK+PNNEVL K
Sbjct: 61  TMATLKDSLSQTLARFYLLAGRIVGKSIHCSDQGAVFIEASVSCNMSHILKQPNNEVLRK 120

Query: 121 LLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTNKDS 180
           LLPCS+ C+ P E+  Q+VVQAN+F CGG+AISLCLLHKL+D AT + FL SWASTNK S
Sbjct: 121 LLPCSLLCSNPAEESPQVVVQANIFSCGGIAISLCLLHKLMDGATFTSFLTSWASTNKGS 180

Query: 181 LNSSPKMSCSDYKAFSSLFPQTNSLPFHPQVLHSSSDRKRRLRRFVFRGKAISDLKARAR 240
           L+SS  M C DYKAFSSLFPQTN LPFH Q L   ++RKR LRRFVF  K++ +LKA+A 
Sbjct: 181 LDSSSNMICYDYKAFSSLFPQTNLLPFH-QKLAKPANRKRCLRRFVFGSKSLLNLKAKAE 240

Query: 241 SSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPEVSVGN 300
           S  VPNP+ VEALTGFIWKH M A+    S      STLSHVVNLR RM+P+LPEVSVGN
Sbjct: 241 SRAVPNPSCVEALTGFIWKHAMMAADCQGS------STLSHVVNLRGRMKPALPEVSVGN 300

Query: 301 VMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLM--GDKGTRGVLKLIGEM 360
           +MWGA+AH +SA E +E  L  LVGLLRQSFE+IN D+++ ++   DKG   VLK +GEM
Sbjct: 301 IMWGAVAH-SSAMERLE--LRALVGLLRQSFEEINKDYIERMLIGSDKGFEAVLKFVGEM 360

Query: 361 NKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSGDGVE 420
           NK+   + +YYF TSWRNM LNEVDFGWG+PAWI IA D  E+M N+IVL+D V GDG+E
Sbjct: 361 NKW--GMSNYYFCTSWRNMKLNEVDFGWGRPAWIAIAWDTNELMANVIVLVDGVMGDGIE 420

Query: 421 AWMVLDEKEMKFLEQNPQFLQFALLNPSV 448
           AW++LDEKEM+ LEQNPQFL+FALLNPS+
Sbjct: 421 AWILLDEKEMEILEQNPQFLEFALLNPSI 431

BLAST of Lag0021673 vs. ExPASy TrEMBL
Match: A0A1S3BXK4 (LOW QUALITY PROTEIN: salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103494516 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 7.0e-158
Identity = 298/469 (63.54%), Postives = 371/469 (79.10%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           MEVK+LS+ETI PSSPTPPHL+T QLSL+DQLSPM Y PLL F+PMKK+    +  H K 
Sbjct: 42  MEVKVLSKETIIPSSPTPPHLQTLQLSLLDQLSPMLYIPLLLFYPMKKS--YQHQDHNK- 101

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLIK 120
            +ATLKTSLS+TLSRF+LLAGRII +SIHC+DKGA+F+EA+++ NM ++LKEPNNEVL K
Sbjct: 102 AIATLKTSLSKTLSRFYLLAGRIIGKSIHCNDKGAVFMEATINSNMFDILKEPNNEVLTK 161

Query: 121 LLPCSVFC-TEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTNKD 180
           LLPCS+ C T+P+E+Y QIVVQAN+F+CGG+AISLCLLHKLID AT  CFLRSWA+TN++
Sbjct: 162 LLPCSLLCNTKPIEEYPQIVVQANIFECGGIAISLCLLHKLIDAATFCCFLRSWATTNRE 221

Query: 181 SL---NSSP--KMSCSDYKAFSSLFPQTNSLPFHPQVLHSSS---------DRKRRLRRF 240
           SL   +SSP   M C DYK+FSSLFPQTN LPFH  ++++           +RKR  +RF
Sbjct: 222 SLSLDHSSPNNNMVCFDYKSFSSLFPQTNLLPFHQSLINNDKXVVPPSSIFNRKRHFQRF 281

Query: 241 VFRGKAISDLKARARSSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNL 300
           VFR +AI DLKA+A+S ++PNPT VE LT FIWK++MK +  G S R    STLSHVVN+
Sbjct: 282 VFRSEAILDLKAKAKSCDIPNPTCVETLTCFIWKYLMKVADDGDSQRP---STLSHVVNI 341

Query: 301 RKRMEPSLPEVSVGNVMWGAIAHYNSA--NESIE-VKLSELVGLLRQSFEKINSDFVKGL 360
           RK +EPSL EVS+GN+MWG +AH+ S   NE  E ++LS+LV LLRQSF+KIN D++K L
Sbjct: 342 RKMLEPSLGEVSLGNIMWGTVAHHFSTTRNEEFEGLELSKLVSLLRQSFKKINKDYIKEL 401

Query: 361 M--GDKGTR-GVLKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLE 420
           +  GDK  R GV+KL+GE+NK+P  + +YYFFTSW+N+ LNEVDFGWGKP W  IAGD  
Sbjct: 402 IMGGDKERRNGVMKLVGEINKWP--ISNYYFFTSWKNLKLNEVDFGWGKPLWSAIAGDPN 461

Query: 421 EIMTNIIVLLDTVSGDG-VEAWMVLDEKEMKFLEQNPQFLQFALLNPSV 448
           E+M NIIVL+D V  DG  EAW++LDEKEM+ LEQ PQFL+FALLNPS+
Sbjct: 462 EMMGNIIVLVDNVLDDGSTEAWILLDEKEMQLLEQIPQFLEFALLNPSI 502

BLAST of Lag0021673 vs. TAIR 10
Match: AT3G26040.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.7e-71
Identity = 172/456 (37.72%), Postives = 261/456 (57.24%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           M V ++SR+ IKPSSPTP HLK F+LSL++QL P  + P++FF+         N + P  
Sbjct: 1   MRVDVVSRDIIKPSSPTPNHLKKFKLSLLEQLGPTIFGPMVFFY------SANNSIKPTE 60

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDR-SIHCSDKGAIFLEASVSCNMLEVLKEPNNEVLI 120
            L  LK SLSETL+ F+ LAGR+    SI C+D GA FLEA V+  +  +L EP+++ L 
Sbjct: 61  QLQMLKKSLSETLTHFYPLAGRLKGNISIDCNDSGADFLEARVNSPLSNLLLEPSSDSLQ 120

Query: 121 KLLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWAS-TNK 180
           +L+P SV   E   +   ++ QA+ F+CG ++I +C+ HKL D  ++  F++SWA+ +++
Sbjct: 121 QLIPTSVDSIE--TRTRLLLAQASFFECGSMSIGVCISHKLADATSIGLFMKSWAAISSR 180

Query: 181 DSLNSSPKMSCSDYKAF-SSLFPQTNSLP-FHPQVLHSSSDRKRRLRRFVFRGKAISDLK 240
            S+ +         K F    F +T+  P   P+++ + +  K    RF+F   +I  L+
Sbjct: 181 GSIKTIGAPVFDTVKIFPPGNFSETSPAPVVEPEIMMNQTLSK----RFIFDSSSIQALQ 240

Query: 241 ARARSSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPEV 300
           A+A S  V  PT VEA++  IWK  MKA+   T S + K S L++ V+LR R+ P   + 
Sbjct: 241 AKASSFEVNQPTRVEAVSALIWKSAMKATR--TVSGTSKPSILANSVSLRSRVSPPFTKN 300

Query: 301 SVGNVMWGAIAHYNSANESI-EVKLSELVGLLRQSFEKINSDFVKGLMGDKGTRGVL--- 360
           S+GN++    A    A E I + KL  LV  +R++ ++     +  L+G+     ++   
Sbjct: 301 SIGNLVSYFAA---KAEEGINQTKLQTLVSKIRKAKQRFRDIHIPKLVGNPNATEIICSY 360

Query: 361 -KLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDT 420
            K  G+M    S   D+Y F+S     L E DFGWGKP W+G     ++   NI+ LLDT
Sbjct: 361 QKEAGDM--IASGDFDFYIFSSACRFGLYETDFGWGKPVWVGFPSVRQK---NIVTLLDT 420

Query: 421 VSGDGVEAWMVLDEKEMKFLEQNPQFLQFALLNPSV 448
               G+EAW+ L+E+EM   EQ+ + LQFA LNPSV
Sbjct: 421 KEAGGIEAWVNLNEQEMNLFEQDRELLQFASLNPSV 434

BLAST of Lag0021673 vs. TAIR 10
Match: AT4G15390.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 251.5 bits (641), Expect = 1.3e-66
Identity = 162/458 (35.37%), Postives = 255/458 (55.68%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           M+V+ +S+E IKPSSPTP +L+T QLS+ D + P  YT    F+   KND +  + H  +
Sbjct: 6   MKVETISKEIIKPSSPTPNNLQTLQLSIYDHILPPVYTVAFLFY--TKNDLISQE-HTSH 65

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASV-SCNMLEVLKEPNNEVLI 120
               LKTSLSETL++F+ LAGRI   ++ C+D+GAIF++A V +C + E LK P+ + L 
Sbjct: 66  ---KLKTSLSETLTKFYPLAGRITGVTVDCTDEGAIFVDARVNNCPLTEFLKCPDFDALQ 125

Query: 121 KLLPCSVFCTEPV--EKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTN 180
           +LLP  V     V    +  ++V+A  F CGG+AI +C+ HK+ D A++S F+RSWA+T 
Sbjct: 126 QLLPLDVVDNPYVAAATWPLLLVKATYFGCGGMAIGICITHKIADAASISTFIRSWAATA 185

Query: 181 KDSLNSSPKMSCSDYKAFSSLFPQTNSLPFHPQVLHSSSDRKRRLRRFVFRGKAISDLKA 240
           +   N +  M  S   A ++ +P  N   F       +  R    +RFVF    + DL+ 
Sbjct: 186 RGE-NDAAAME-SPVFAGANFYPPANE-AFKLPADEQAGKRSSITKRFVFEASKVEDLRT 245

Query: 241 RARS-SNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPEV 300
           +A S   V  PT VE++T  IWK  + +S + T         L  + NLR ++   L E 
Sbjct: 246 KAASEETVDQPTRVESVTALIWKCFVASSKTTTCDH----KVLVQLANLRSKIPSLLQES 305

Query: 301 SVGNVMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFV-KGLMGDK----GTRGV 360
           S+GN+M+ ++    S     EVK+ E V  LR+  E++ +  + +G   D     G++  
Sbjct: 306 SIGNLMFSSVV--LSIGRGGEVKIEEAVRDLRKKKEELGTVILDEGGSSDSSSMIGSKLA 365

Query: 361 LKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDT 420
             ++   ++   E  + Y  +SW  + L E  FGW  P W  + G++  ++ N+ +L+D+
Sbjct: 366 NLMLTNYSRLSYETHEPYTVSSWCKLPLYEASFGWDSPVW--VVGNVSPVLGNLAMLIDS 425

Query: 421 VSGDGVEAWMVLDEKEMKFLEQNPQFLQFALLNPSVAI 450
             G G+EA++ L E+ M   EQNP+ L FA +NPSV +
Sbjct: 426 KDGQGIEAFVTLPEENMSSFEQNPELLAFATMNPSVLV 446

BLAST of Lag0021673 vs. TAIR 10
Match: AT5G47950.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 236.1 bits (601), Expect = 5.4e-62
Identity = 163/455 (35.82%), Postives = 247/455 (54.29%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           M+V+ + +E IKPS+ TP  L T QLS++D L P  Y     F+     DD+   +  + 
Sbjct: 4   MKVETIGKEIIKPSATTPTDLSTLQLSIMDILMPPVYAVAFLFY---TKDDL---ISQEQ 63

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASV-SCNMLEVLKEPNNEVLI 120
              TLKTSLSE L++F  LAGR+   +I  +D+GA+F+EA V +C++   L+ P+ E L 
Sbjct: 64  TSHTLKTSLSEILTKFHPLAGRVNGVTIKSTDEGAVFVEARVDNCDLSGFLRSPDTESLK 123

Query: 121 KLLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWAST--N 180
           +LLP      EP   +  ++V+A  F CGG+AI LC+ H+L D A+LS FL++WA+T   
Sbjct: 124 QLLPVD---DEPAPTWPLLLVKATYFRCGGMAIGLCISHRLADAASLSIFLQAWAATARG 183

Query: 181 KDSLNSSPKMSCSDYKAFSSLFPQTN-SLPFHPQVLHSSSDRKRRLRRFVFRGKAISDLK 240
           +  L +SP   CS     + L+P  N ++    +V++ +S  K    RFVF    I +L+
Sbjct: 184 ESDLVASPDF-CS-----TKLYPAANEAIKIPGEVVNRTSVTK----RFVFVASKIEELR 243

Query: 241 ARARSSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPEV 300
            +  S  VP PT V+++T  IWK V+ AST     ++     L    NLR ++   L E 
Sbjct: 244 NKVASDVVPRPTRVQSVTSLIWKCVVTASTDTIREKA-----LFQPANLRTKIPSLLSEN 303

Query: 301 SVGNVMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLMGDKGTRGVLKLIG 360
            +GN+++ ++     A   I   + EL     Q   +  S  V+   G   T G  +L G
Sbjct: 304 QIGNLLFNSLILDGKAGMDIVETVKEL-----QKRAEDLSGLVQDEEGSSMTIG-WRLFG 363

Query: 361 EM--NKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTVSG 420
           E+  +KF  E  D +  TSW  + L +  FGWG P W  +AG +   +  + VL+D+  G
Sbjct: 364 EIINSKFNREDHDMHSVTSWCKIPLYDACFGWGSPVW--VAGSVSPNLDYVTVLIDSKDG 423

Query: 421 DGVEAWMVLDEKEMKFLEQNPQFLQFALLNPSVAI 450
            G+EAW+ L ++ M  LEQ+ + L FA  NPSV I
Sbjct: 424 QGIEAWVTLPQENMLLLEQSTELLAFASPNPSVLI 426

BLAST of Lag0021673 vs. TAIR 10
Match: AT3G30280.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 232.6 bits (592), Expect = 6.0e-61
Identity = 152/457 (33.26%), Postives = 255/457 (55.80%), Query Frame = 0

Query: 1   MEVKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKN 60
           M+V+ +++E IKPSS TP  L+T QLS+ D + P  YT    F+     DD+   + P+ 
Sbjct: 4   MKVETIAKEIIKPSSTTPNDLQTLQLSIYDHILPPVYTVAFLFY---TKDDL---ISPEQ 63

Query: 61  LLATLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNML-EVLKEPNNEVLI 120
               LKTSL+ETL++F+ LAGRI   +I C+D+GA+F++A V+   L + L+ P+ + L 
Sbjct: 64  SSHKLKTSLAETLTKFYPLAGRIKGVTIDCNDEGAVFVDARVNNYPLSDFLRSPDFKTLQ 123

Query: 121 KLLPCSVFCT--EPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWASTN 180
           +LLP  V     E    +  ++V+A  F CGG+AI LC+ HK+ D  ++S F++SWA+  
Sbjct: 124 QLLPLDVIDDPYEAATTWPLLLVKATYFPCGGMAIGLCITHKIADATSISTFIQSWAAMA 183

Query: 181 KDSLNSSPKMSCSDYKAFSSLFPQTNSLPFHPQVLHSSSDRKRRLRRFVFRGKAISDLKA 240
           +     +  +      A ++ +P  N L F   V  +++   +  +RFVF    + +L+ 
Sbjct: 184 R---GEAGDIVADPVFAAANFYPPANEL-FKFPVDENANKISQITKRFVFSASKLEELRT 243

Query: 241 RARSSN-VPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNLRKRMEPSLPEV 300
           +A S + V  P  VE++T  +WK  + A++S T++   K+  L    NLR ++   L E 
Sbjct: 244 KAASEDFVGRPKRVESVTALLWKAFVAAASSNTNACDMKV--LIQPSNLRPKIPSLLAES 303

Query: 301 SVGNVMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLMGDK----GTRGVL 360
            +GN+M+ ++    S  E  E+K+ + V  LR+  + +  + +K   G      G++   
Sbjct: 304 LIGNIMFSSVVLGISQQE--EIKIEKAVRDLRKQGDDL-QNVIKEEGGSSSAMIGSKLAN 363

Query: 361 KLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNIIVLLDTV 420
            ++   +K   E  + Y  +SW  + L E  FGWG P W  IAG++  ++ N+ +L+D+ 
Sbjct: 364 LMLTNYSKLSYETHEPYTVSSWCKLPLYEASFGWGSPVW--IAGNVAPMLENVTMLIDSK 423

Query: 421 SGDGVEAWMVLDEKEMKFLEQNPQFLQFALLNPSVAI 450
            G G+EA++ L E+ M  LEQN + L FA +NPSV +
Sbjct: 424 DGQGIEAFVTLPEENMLSLEQNTELLAFASVNPSVLV 443

BLAST of Lag0021673 vs. TAIR 10
Match: AT1G24420.1 (HXXXD-type acyl-transferase family protein )

HSP 1 Score: 228.8 bits (582), Expect = 8.7e-60
Identity = 161/464 (34.70%), Postives = 250/464 (53.88%), Query Frame = 0

Query: 3   VKILSRETIKPSSPTPPHLKTFQLSLIDQLSPMFYTPLLFFFPMKKNDDVPNDVHPKNLL 62
           V+ILSRE +KPSSPTP   +   LSL+D LS   YT  L F+             P+NLL
Sbjct: 5   VEILSREIVKPSSPTPDDKRILNLSLLDILSSPMYTGALLFYA----------ADPQNLL 64

Query: 63  A--------TLKTSLSETLSRFFLLAGRIIDRSIHCSDKGAIFLEASVSCNMLEVLKEPN 122
                     LK SLS+TL  F+ LAGRII   + C+D+GA+F+EA V   + E LK P 
Sbjct: 65  GFSTEETSLKLKKSLSKTLPIFYPLAGRIIGSFVECNDEGAVFIEARVDHLLSEFLKCPV 124

Query: 123 NEVLIKLLPCSVFCTEPVEKYAQIVVQANVFDCGGVAISLCLLHKLIDVATLSCFLRSWA 182
            E L  L+P      E V  +  +++QAN F CGG+ I++C+ HK+ D  +L+ F+R WA
Sbjct: 125 PESLELLIPVEAKSREAV-TWPVLLIQANFFSCGGLVITICVSHKITDATSLAMFIRGWA 184

Query: 183 STNKD-SLNSSPKMSCSDYKAFSSLFPQ-TNSLPFHPQVLHSSSDRKRRL-------RRF 242
            +++   +   P  + S+      +FP+  + LP  P       DRK  +       +RF
Sbjct: 185 ESSRGLGITLIPSFTASE------VFPKPLDELPSKPM------DRKEEVEEMSCVTKRF 244

Query: 243 VFRGKAISDLKARARSSNVPNPTSVEALTGFIWKHVMKASTSGTSSRSEKLSTLSHVVNL 302
           VF    I  L+A+A  + V NPT VEA+T   W+ V K S    SS + + S L  +VNL
Sbjct: 245 VFDASKIKKLRAKASRNLVKNPTRVEAVTALFWRCVTKVSR--LSSLTPRTSVLQILVNL 304

Query: 303 RKRMEPSLPEVSVGNVMWGAIAHYNSANESIEVKLSELVGLLRQSFEKINSDFVKGLMGD 362
           R +++ SL E ++GN++  ++    +   +IE ++ ++V  +R++ E  + +  +     
Sbjct: 305 RGKVD-SLCENTIGNML--SLMILKNEEAAIE-RIQDVVDEIRRAKEIFSLNCKEMSKSS 364

Query: 363 KGTRGVLKLIGEMNKFPSEVRDYYFFTSWRNMDLNEVDFGWGKPAWIGIAGDLEEIMTNI 422
                +L+ IG++    +E+ D +   SW  + L + DFGWGKP W  + G       N+
Sbjct: 365 SRIFELLEEIGKVYGRGNEM-DLWMSNSWCKLGLYDADFGWGKPVW--VTGRGTSHFKNL 424

Query: 423 IVLLDTVSGDGVEAWMVLDEKEMKFLEQNPQFLQFALLNPSVAI 450
           ++L+DT  G+G+EAW+ L E++M   E + + L+ A LNP V I
Sbjct: 425 MLLIDTKDGEGIEAWITLTEEQMSLFECDQELLESASLNPPVLI 436

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138192.11.1e-17067.10BAHD acyltransferase BIA1-like [Momordica charantia][more]
XP_004152093.12.7e-16464.83stemmadenine O-acetyltransferase [Cucumis sativus][more]
KAE8649121.11.3e-16364.35hypothetical protein Csa_015082 [Cucumis sativus][more]
KAG6582063.19.7e-16266.59Salutaridinol 7-O-acetyltransferase, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_022956252.11.3e-16166.59salutaridinol 7-O-acetyltransferase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q94FT43.3e-5633.33Salutaridinol 7-O-acetyltransferase OS=Papaver somniferum OX=3469 GN=SALAT PE=1 ... [more]
I3PLR41.1e-5131.77(13S,14R)-1,13-dihydroxy-N-methylcanadine 13-O-acetyltransferase AT1 OS=Papaver ... [more]
O233931.9e-5132.75BAHD acyltransferase BIA1 OS=Arabidopsis thaliana OX=3702 GN=BIA1 PE=2 SV=1[more]
A0A2P1GIW73.2e-5132.57Stemmadenine O-acetyltransferase OS=Catharanthus roseus OX=4058 GN=SAT PE=1 SV=1[more]
Q70PR71.8e-4631.26Vinorine synthase OS=Rauvolfia serpentina OX=4060 GN=ACT PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CAD95.5e-17167.10BAHD acyltransferase BIA1-like OS=Momordica charantia OX=3673 GN=LOC111009425 PE... [more]
A0A0A0KXH21.3e-16464.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G016510 PE=3 SV=1[more]
A0A6J1GVT56.1e-16266.59salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1GVI62.3e-16166.37salutaridinol 7-O-acetyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A1S3BXK47.0e-15863.54LOW QUALITY PROTEIN: salutaridinol 7-O-acetyltransferase-like OS=Cucumis melo OX... [more]
Match NameE-valueIdentityDescription
AT3G26040.11.7e-7137.72HXXXD-type acyl-transferase family protein [more]
AT4G15390.11.3e-6635.37HXXXD-type acyl-transferase family protein [more]
AT5G47950.15.4e-6235.82HXXXD-type acyl-transferase family protein [more]
AT3G30280.16.0e-6133.26HXXXD-type acyl-transferase family protein [more]
AT1G24420.18.7e-6034.70HXXXD-type acyl-transferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 1..210
e-value: 1.4E-51
score: 176.9
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 221..439
e-value: 1.7E-55
score: 189.4
IPR003480TransferasePFAMPF02458Transferasecoord: 1..437
e-value: 6.7E-76
score: 255.7
NoneNo IPR availablePANTHERPTHR31623F21J9.9coord: 4..447
NoneNo IPR availablePANTHERPTHR31623:SF59HXXXD-TYPE ACYL-TRANSFERASE FAMILY PROTEINcoord: 4..447

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0021673.1Lag0021673.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups