ClCG07G000730 (gene) Watermelon (Charleston Gray) v2.5

Overview


Name	ClCG07G000730
Type	gene
Organism	Citrullus lanatus subsp. vulgaris cv. Charleston Gray (Watermelon (Charleston Gray) v2.5)
Description	Trypsin inhibitor
Location	CG_Chr07: 803928 .. 804245 (+)
RNA-Seq Expression	ClCG07G000730
Synteny	ClCG07G000730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAAGAAATTGTTATTCCTTCAGGTTAGTAAGACTTCTCCTTCCCACTTGGGTGTTTTCAAATAATTATTTTTTGTTGTCCTTTTCAGTATTTACATTTTCACCATTCAATTTATTTAGGGAAAAATCCTCGTGGCCCGAACTCGTATTCGTCGAATCTTCCACTGCAGTACATATTATAGAGAGAGAATCCTAAAGTGAAGACAATCGTGCTATTAGCTGGATCTCCAGTTACTGAAGATTTGAGATTTGATCGAGTTCGAGTTTTTGTTAACATAAATAGTGTGGTGGTTATTATTCCTACAATTGGTTGA

mRNA sequence

ATGGCTGAAGAAATTGTTATTCCTTCAGTATTTACATTTTCACCATTCAATTTATTTAGGGAAAAATCCTCGTGGCCCGAACTCGTATTCGTCGAATCTTCCACTGCAAGAGAGAATCCTAAAGTGAAGACAATCGTGCTATTAGCTGGATCTCCAGTTACTGAAGATTTGAGATTTGATCGAGTTCGAGTTTTTGTTAACATAAATAGTGTGGTGGTTATTATTCCTACAATTGGTTGA

Coding sequence (CDS)

ATGGCTGAAGAAATTGTTATTCCTTCAGTATTTACATTTTCACCATTCAATTTATTTAGGGAAAAATCCTCGTGGCCCGAACTCGTATTCGTCGAATCTTCCACTGCAAGAGAGAATCCTAAAGTGAAGACAATCGTGCTATTAGCTGGATCTCCAGTTACTGAAGATTTGAGATTTGATCGAGTTCGAGTTTTTGTTAACATAAATAGTGTGGTGGTTATTATTCCTACAATTGGTTGA

Protein sequence

MAEEIVIPSVFTFSPFNLFREKSSWPELVFVESSTARENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIPTIG

Homology

[Close]

BLAST of ClCG07G000730 vs. NCBI nr
Match: XP_011654473.1 (inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetical protein Csa_018430 [Cucumis sativus])

HSP 1 Score: 77.0 bits (188), Expect = 8.0e-11
Identity = 49/85 (57.65%), Postives = 56/85 (65.88%), Query Frame = 0


Query: 1  MAEE-IVIPSVFTFSPFNLFREKSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVT 60
          MAEE IV PS            KSSWPELVFV+SS A     R+ P VK +VLLAGSPVT
Sbjct: 1  MAEEKIVFPS----------SGKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVT 60

Query: 61 EDLRFDRVRVFVNINSVVVIIPTIG 80
          EDLR +RVR+FVN+N  VV +P  G
Sbjct: 61 EDLRPNRVRIFVNMNDRVVEVPRTG 75

[Close]

BLAST of ClCG07G000730 vs. NCBI nr
Match: KAA8527727.1 (hypothetical protein F0562_035404 [Nyssa sinensis])

HSP 1 Score: 70.5 bits (171), Expect = 7.5e-09
Identity = 36/63 (57.14%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          KSSWPELV V+  TA     RENP V  +++L GSPVT D R DRVRV+VN N +V ++P
Sbjct: 8  KSSWPELVGVQGETATATIERENPLVTALIVLEGSPVTPDFRCDRVRVYVNENGIVTLVP 67

[Close]

BLAST of ClCG07G000730 vs. NCBI nr
Match: AES61050.1 (inhibitor of trypsin and hageman factor-like protein [Medicago truncatula] >RHN80340.1 putative proteinase inhibitor I13, potato inhibitor I [Medicago truncatula])

HSP 1 Score: 68.2 bits (165), Expect = 3.7e-08
Identity = 37/63 (58.73%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          KSSWPELV VE   A     REN  VK I++L GS VT+DLRFDRVRV+V+   +V  +P
Sbjct: 9  KSSWPELVGVEGKVAEATIQRENASVKAIIVLEGSSVTDDLRFDRVRVWVDKEGIVTQVP 68

[Close]

BLAST of ClCG07G000730 vs. NCBI nr
Match: VVA34150.1 (PREDICTED: ase inhibitor [Prunus dulcis])

HSP 1 Score: 65.1 bits (157), Expect = 3.1e-07
Identity = 34/62 (54.84%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 79
          K SWPELV  E   A     RENP V  ++L  G+P+T+DLRFDRVRV+VN N +V  IP
Sbjct: 34 KDSWPELVGAEGKVAKATIERENPLVTALILHIGAPITQDLRFDRVRVWVNTNGIVTWIP 93

[Close]

BLAST of ClCG07G000730 vs. NCBI nr
Match: XP_020423680.1 (glu S.griseus protease inhibitor [Prunus persica] >ONI26577.1 hypothetical protein PRUPE_1G032100 [Prunus persica])

HSP 1 Score: 65.1 bits (157), Expect = 3.1e-07
Identity = 34/62 (54.84%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 79
          K SWPELV  E   A     RENP V  ++L  G+P+T+DLRFDRVRV+VN N +V  IP
Sbjct: 34 KDSWPELVGAEGKVAKATIERENPLVTALILHLGAPITQDLRFDRVRVWVNTNGIVTWIP 93

[Close]

BLAST of ClCG07G000730 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 5.0e-08
Identity = 32/63 (50.79%), Postives = 41/63 (65.08%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          KSSWP LV V  S A     R+NP VK ++L  G+PVT+D R +RVR++VN   +VV  P
Sbjct: 6  KSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLVVSPP 65

[Close]

BLAST of ClCG07G000730 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 6.6e-08
Identity = 32/63 (50.79%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          K+SWPELV      A      EN  VK IV+  G P+T+DL F+RVRVFV+ N VV  +P
Sbjct: 8  KNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDENRVVTQVP 67

[Close]

BLAST of ClCG07G000730 vs. ExPASy Swiss-Prot
Match: P24076 (Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 9.5e-07
Identity = 29/63 (46.03%), Postives = 38/63 (60.32%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          K SWP+LV    + A     RENP+V+ +++  GSPVT D R DRVRV+V    +V   P
Sbjct: 6  KRSWPQLVGSTGAAAKAVIERENPRVRAVIVRVGSPVTADFRCDRVRVWVTERGIVARPP 65

[Close]

BLAST of ClCG07G000730 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.1e-06
Identity = 30/64 (46.88%), Postives = 40/64 (62.50%), Query Frame = 0


Query: 22  KSSWPELV-----FVESSTARENPKVKTI-VLLAGSPVTEDLRFDRVRVFVNINSVVVII 80
           K SWPEL+     F +    +ENPK+  +  LL GS  TEDLR +RVR+FVN+  +VV  
Sbjct: 56  KESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQT 115

[Close]

BLAST of ClCG07G000730 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.8e-06
Identity = 35/79 (44.30%), Postives = 46/79 (58.23%), Query Frame = 0


Query: 6   VIPSVFTFSPFNLFREKSSWPELVFVESSTA-----RENPKVKTI-VLLAGSPVTEDLRF 65
           VI  +  F   +  + K  WPEL+ V +  A     +ENP +  I +LL GSPVT+D R 
Sbjct: 32  VIKLLKEFESDSRCKGKQFWPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRC 91

Query: 66  DRVRVFVNINSVVVIIPTI 79
           DRVR+FVNI   VV IP +
Sbjct: 92  DRVRLFVNILGDVVQIPRV 110

[Close]

BLAST of ClCG07G000730 vs. ExPASy TrEMBL
Match: A0A0A0KPI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.9e-11
Identity = 49/85 (57.65%), Postives = 56/85 (65.88%), Query Frame = 0


Query: 1  MAEE-IVIPSVFTFSPFNLFREKSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVT 60
          MAEE IV PS            KSSWPELVFV+SS A     R+ P VK +VLLAGSPVT
Sbjct: 1  MAEEKIVFPS----------SGKSSWPELVFVKSSVAVHWIERDRPDVKPVVLLAGSPVT 60

Query: 61 EDLRFDRVRVFVNINSVVVIIPTIG 80
          EDLR +RVR+FVN+N  VV +P  G
Sbjct: 61 EDLRPNRVRIFVNMNDRVVEVPRTG 75

[Close]

BLAST of ClCG07G000730 vs. ExPASy TrEMBL
Match: A0A5J5AE65 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_035404 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.6e-09
Identity = 36/63 (57.14%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          KSSWPELV V+  TA     RENP V  +++L GSPVT D R DRVRV+VN N +V ++P
Sbjct: 8  KSSWPELVGVQGETATATIERENPLVTALIVLEGSPVTPDFRCDRVRVYVNENGIVTLVP 67

[Close]

BLAST of ClCG07G000730 vs. ExPASy TrEMBL
Match: G7I4R4 (Inhibitor of trypsin and hageman factor-like protein OS=Medicago truncatula OX=3880 GN=MTR_1g075380 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.8e-08
Identity = 37/63 (58.73%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          KSSWPELV VE   A     REN  VK I++L GS VT+DLRFDRVRV+V+   +V  +P
Sbjct: 9  KSSWPELVGVEGKVAEATIQRENASVKAIIVLEGSSVTDDLRFDRVRVWVDKEGIVTQVP 68

[Close]

BLAST of ClCG07G000730 vs. ExPASy TrEMBL
Match: A0A5E4G3A3 (PREDICTED: ase inhibitor OS=Prunus dulcis OX=3755 GN=ALMOND_2B014310 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.5e-07
Identity = 34/62 (54.84%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 79
          K SWPELV  E   A     RENP V  ++L  G+P+T+DLRFDRVRV+VN N +V  IP
Sbjct: 34 KDSWPELVGAEGKVAKATIERENPLVTALILHIGAPITQDLRFDRVRVWVNTNGIVTWIP 93

[Close]

BLAST of ClCG07G000730 vs. ExPASy TrEMBL
Match: A0A251QS16 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G032100 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.5e-07
Identity = 34/62 (54.84%), Postives = 41/62 (66.13%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 79
          K SWPELV  E   A     RENP V  ++L  G+P+T+DLRFDRVRV+VN N +V  IP
Sbjct: 34 KDSWPELVGAEGKVAKATIERENPLVTALILHLGAPITQDLRFDRVRVWVNTNGIVTWIP 93

[Close]

BLAST of ClCG07G000730 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.0e-08
Identity = 33/63 (52.38%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          K+SWPEL       A     RENP V   V+L GSPVT D R DRVRVFV+ N +VV  P
Sbjct: 8  KNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVKTP 67

[Close]

BLAST of ClCG07G000730 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 48.5 bits (114), Expect = 2.8e-06
Identity = 29/63 (46.03%), Postives = 38/63 (60.32%), Query Frame = 0


Query: 22 KSSWPELVFVESSTA-----RENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          KSSWPEL+  +   A     RENPK+K +++L G+ V E     RV V+VN   +VV IP
Sbjct: 37 KSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQIP 96

[Close]

BLAST of ClCG07G000730 vs. TAIR 10
Match: AT2G38900.2 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 41.6 bits (96), Expect = 3.5e-04
Identity = 30/74 (40.54%), Postives = 42/74 (56.76%), Query Frame = 0


Query: 11 FTFSPFNLFREKSSWPELV-----FVESSTARENPKVKTIVLLAGSPVTEDLRFDRVRVF 70
          F FS F++   K+SWPEL+     +  S    EN  +  +V+  G+ VTEDL   RVRV+
Sbjct: 17 FYFSFFDI--GKNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVW 76

Query: 71 VNINSVVVIIPTIG 80
          V+   +VV  PT G
Sbjct: 77 VDEIRIVVRNPTAG 88

[Close]

BLAST of ClCG07G000730 vs. TAIR 10
Match: AT2G38900.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 40.4 bits (93), Expect = 7.7e-04
Identity = 26/63 (41.27%), Postives = 36/63 (57.14%), Query Frame = 0


Query: 22 KSSWPELV-----FVESSTARENPKVKTIVLLAGSPVTEDLRFDRVRVFVNINSVVVIIP 80
          K+SWPEL+     +  S    EN  +  +V+  G+ VTEDL   RVRV+V+   +VV  P
Sbjct: 11 KNSWPELLGTNGDYAASVIKGENSSLNVVVVSDGNYVTEDLSCYRVRVWVDEIRIVVRNP 70

The following BLAST results are available for this feature:

BLAST of ClCG07G000730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654473.1	8.0e-11	57.65	inhibitor of trypsin and hageman factor [Cucumis sativus] >KGN49621.1 hypothetic...	[more]
KAA8527727.1	7.5e-09	57.14	hypothetical protein F0562_035404 [Nyssa sinensis]	[more]
AES61050.1	3.7e-08	58.73	inhibitor of trypsin and hageman factor-like protein [Medicago truncatula] >RHN8...	[more]
VVA34150.1	3.1e-07	54.84	PREDICTED: ase inhibitor [Prunus dulcis]	[more]
XP_020423680.1	3.1e-07	54.84	glu S.griseus protease inhibitor [Prunus persica] >ONI26577.1 hypothetical prote...	[more]

BLAST of ClCG07G000730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	5.0e-08	50.79	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q6XNP7	6.6e-08	50.79	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
P24076	9.5e-07	46.03	Glu S.griseus protease inhibitor OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P20076	2.1e-06	46.88	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]
P16231	2.8e-06	44.30	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]

BLAST of ClCG07G000730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KPI0	3.9e-11	57.65	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G027950 PE=3 SV=1	[more]
A0A5J5AE65	3.6e-09	57.14	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_035404 PE=3 SV=1	[more]
G7I4R4	1.8e-08	58.73	Inhibitor of trypsin and hageman factor-like protein OS=Medicago truncatula OX=3...	[more]
A0A5E4G3A3	1.5e-07	54.84	PREDICTED: ase inhibitor OS=Prunus dulcis OX=3755 GN=ALMOND_2B014310 PE=3 SV=1	[more]
A0A251QS16	1.5e-07	54.84	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G032100 PE=3 SV=1	[more]

BLAST of ClCG07G000730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	1.0e-08	52.38	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	2.8e-06	46.03	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.2	3.5e-04	40.54	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38900.1	7.7e-04	41.27	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (Charleston Gray) v2.5
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 18..79 e-value: 1.4E-16 score: 62.6
None	No IPR available	PANTHER	PTHR33091:SF83	ETHYLENE-RESPONSIVE PROTEINASE INHIBITOR 1	coord: 21..79
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 22..79 e-value: 5.0E-15 score: 55.9
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 21..79
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 20..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
ClCG07G000730.2	ClCG07G000730.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity