CaUC03G066080 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC03G066080
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionPectinesterase
LocationCiama_Chr03: 31791209 .. 31793129 (+)
RNA-Seq ExpressionCaUC03G066080
SyntenyCaUC03G066080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTATAGAGATGATGATTCTCAGAACAAGAAAAGATACGCCATCATCGGTGTCTCGTCCATGTTACTAGTGGCGATGGTGGTGGCGGTGACGGTTGGCGTTAACCTAAACCAAGACGAAACGAGCGAACCAGCTACCGGAAACAAAGCCCATGAAATATCTGCTTCAGTGAAAGCCATTAAAGCCATTTGTCAACCTACTGATTATAAACAAGAATGTGTTGCTAGTCTCAAGGCTGCTGGCAACAACTCTTCAGACCCAAAGGAATTGGTTCAAGCTGGCTTCAAGGCTGCCATGAAGTTGATCCAAGCTGCTGCTAATAAATCTGTCACCTTGAAAGAGCTTGAGAAAGACCCTAGAACCTCCAAGGCCCTTGCTGGTTGTAAAGAGCTCATGGATTTTGCTGTTGACGAGCTTAAATACTCGATGAACAAGCTCGGCGAGTTTGATATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTCAGTGCTACCATTACTTATCAGGTAATCTAGCTAGGCCAAATGTTTAAGTTTGGTTAACCTTAAAAGTTGATGGATTACTGTAAATTTATAATTTGTTCTATGTTTGTATTGGTTTAGGAAACTTGCTTGGATGGGTTCGAGAACACAACCGGAAATGCGGCAGAGAAGATGAAGAAGGCATTGAAGACATCAATGCGGTTGAGCAGCAATGGGTTGGCCATGGTGTCGCAAATTTCGTCCATGTTGACAGAGTTGCAGATTCCAGGGATCAGCCGAAGGCGACTTCTCGAGATTCCGGTTCTTGGGCACGATGATTACCCCGACTGGGCGAACCCTGGGATGCGACGACTCCTAGCTGCCGGGACGAAAGTGAAGCCAAACGTTGTGGTGGCCAAGGATGGAAGTGGTCAGTTCAGGACCATTCAAGAGGCTCTTGACAAAGTTCCAAAGAGGAAGAACAATGCAACCTTTGTGATTCATATCAAGGCGGGAGTGTACAAGGAATATCTCATCATCAAAAAATCTCTCACCCATTTGATGCTCATTGGTGATGGCCCCAAAAAGACCATTATCACTGGAAGTAAAAACTTCATTGATGGCACACCCACCTTCAAAACTGCAACAGTTGGTAAGTATACTTAGAATTTAATCCTCATAATTTGAACGACGAGTTAATTAACAGATTGACTTCTTGTATGTTATATATACAGCTGTGACAGCAGAACACTTTATGGCAAGAGATATCGGGTTCGAAAACACAGCAGGGCCACATAAGCACCAAGCAGTTGCGCTACGAGTCCAAGCCGACAAAGCCGTATTCTACAACTGCGAAATGCATGGCTACCAAGACACTCTCTACGTCCACACCATGCGTCAGTTCTACCGCGATTGCACCATCTCCGGCACCATCGACTTCATCTTCGGTGATGCAGCTGCCATCTTCCAAAGTTGCACCTTCCTAGTCCGTAAGCCCCTTCCAAACCAACAATGCATCGTAACGGCACACGGACGCAAAGAACGCCGCCAACCATCCGCCCTCATCATCCAAAACTGTTCGTTCAAGGCCGACACCGACCTGGCCCCAGTACAAAAACAGTTCCGATCCTTCCTTGGGCGACCATGGAAGGAATATTCGAGAACAATCATCATGGAATCATACATTGGTGATTTGATTCAACCAGAAGGGTGGTTGCCATGGGCTGGAGATTGGGGTTTGAAGACATGTTTTTACACCGAGTTCAACAATTATGGGCCAGGATCTGATAAGTCAAAGAGGGTGAAATGGAGAGGGATTAAGACCATTACGCCACAACATGCTTTGGATTTTACTGCCGGAAGGTTTGTTATGGGGGATAGATGGATTAAGCCCACCGGAGTTCCATATGTCTCCGGCATGACCAGGACCGGCGGAGCTGCTCATTGA

mRNA sequence

ATGGGTTATAGAGATGATGATTCTCAGAACAAGAAAAGATACGCCATCATCGGTGTCTCGTCCATGTTACTAGTGGCGATGGTGGTGGCGGTGACGGTTGGCGTTAACCTAAACCAAGACGAAACGAGCGAACCAGCTACCGGAAACAAAGCCCATGAAATATCTGCTTCAGTGAAAGCCATTAAAGCCATTTGTCAACCTACTGATTATAAACAAGAATGTGTTGCTAGTCTCAAGGCTGCTGGCAACAACTCTTCAGACCCAAAGGAATTGGTTCAAGCTGGCTTCAAGGCTGCCATGAAGTTGATCCAAGCTGCTGCTAATAAATCTGTCACCTTGAAAGAGCTTGAGAAAGACCCTAGAACCTCCAAGGCCCTTGCTGGTTGTAAAGAGCTCATGGATTTTGCTGTTGACGAGCTTAAATACTCGATGAACAAGCTCGGCGAGTTTGATATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTCAGTGCTACCATTACTTATCAGGAAACTTGCTTGGATGGGTTCGAGAACACAACCGGAAATGCGGCAGAGAAGATGAAGAAGGCATTGAAGACATCAATGCGGTTGAGCAGCAATGGGTTGGCCATGGTGTCGCAAATTTCGTCCATGTTGACAGAGTTGCAGATTCCAGGGATCAGCCGAAGGCGACTTCTCGAGATTCCGGTTCTTGGGCACGATGATTACCCCGACTGGGCGAACCCTGGGATGCGACGACTCCTAGCTGCCGGGACGAAAGTGAAGCCAAACGTTGTGGTGGCCAAGGATGGAAGTGGTCAGTTCAGGACCATTCAAGAGGCTCTTGACAAAGTTCCAAAGAGGAAGAACAATGCAACCTTTGTGATTCATATCAAGGCGGGAGTGTACAAGGAATATCTCATCATCAAAAAATCTCTCACCCATTTGATGCTCATTGGTGATGGCCCCAAAAAGACCATTATCACTGGAAGTAAAAACTTCATTGATGGCACACCCACCTTCAAAACTGCAACAGTTGCTGTGACAGCAGAACACTTTATGGCAAGAGATATCGGGTTCGAAAACACAGCAGGGCCACATAAGCACCAAGCAGTTGCGCTACGAGTCCAAGCCGACAAAGCCGTATTCTACAACTGCGAAATGCATGGCTACCAAGACACTCTCTACGTCCACACCATGCGTCAGTTCTACCGCGATTGCACCATCTCCGGCACCATCGACTTCATCTTCGGTGATGCAGCTGCCATCTTCCAAAGTTGCACCTTCCTAGTCCGTAAGCCCCTTCCAAACCAACAATGCATCGTAACGGCACACGGACGCAAAGAACGCCGCCAACCATCCGCCCTCATCATCCAAAACTGTTCGTTCAAGGCCGACACCGACCTGGCCCCAGTACAAAAACAGTTCCGATCCTTCCTTGGGCGACCATGGAAGGAATATTCGAGAACAATCATCATGGAATCATACATTGGTGATTTGATTCAACCAGAAGGGTGGTTGCCATGGGCTGGAGATTGGGGTTTGAAGACATGTTTTTACACCGAGTTCAACAATTATGGGCCAGGATCTGATAAGTCAAAGAGGGTGAAATGGAGAGGGATTAAGACCATTACGCCACAACATGCTTTGGATTTTACTGCCGGAAGGTTTGTTATGGGGGATAGATGGATTAAGCCCACCGGAGTTCCATATGTCTCCGGCATGACCAGGACCGGCGGAGCTGCTCATTGA

Coding sequence (CDS)

ATGGGTTATAGAGATGATGATTCTCAGAACAAGAAAAGATACGCCATCATCGGTGTCTCGTCCATGTTACTAGTGGCGATGGTGGTGGCGGTGACGGTTGGCGTTAACCTAAACCAAGACGAAACGAGCGAACCAGCTACCGGAAACAAAGCCCATGAAATATCTGCTTCAGTGAAAGCCATTAAAGCCATTTGTCAACCTACTGATTATAAACAAGAATGTGTTGCTAGTCTCAAGGCTGCTGGCAACAACTCTTCAGACCCAAAGGAATTGGTTCAAGCTGGCTTCAAGGCTGCCATGAAGTTGATCCAAGCTGCTGCTAATAAATCTGTCACCTTGAAAGAGCTTGAGAAAGACCCTAGAACCTCCAAGGCCCTTGCTGGTTGTAAAGAGCTCATGGATTTTGCTGTTGACGAGCTTAAATACTCGATGAACAAGCTCGGCGAGTTTGATATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTCAGTGCTACCATTACTTATCAGGAAACTTGCTTGGATGGGTTCGAGAACACAACCGGAAATGCGGCAGAGAAGATGAAGAAGGCATTGAAGACATCAATGCGGTTGAGCAGCAATGGGTTGGCCATGGTGTCGCAAATTTCGTCCATGTTGACAGAGTTGCAGATTCCAGGGATCAGCCGAAGGCGACTTCTCGAGATTCCGGTTCTTGGGCACGATGATTACCCCGACTGGGCGAACCCTGGGATGCGACGACTCCTAGCTGCCGGGACGAAAGTGAAGCCAAACGTTGTGGTGGCCAAGGATGGAAGTGGTCAGTTCAGGACCATTCAAGAGGCTCTTGACAAAGTTCCAAAGAGGAAGAACAATGCAACCTTTGTGATTCATATCAAGGCGGGAGTGTACAAGGAATATCTCATCATCAAAAAATCTCTCACCCATTTGATGCTCATTGGTGATGGCCCCAAAAAGACCATTATCACTGGAAGTAAAAACTTCATTGATGGCACACCCACCTTCAAAACTGCAACAGTTGCTGTGACAGCAGAACACTTTATGGCAAGAGATATCGGGTTCGAAAACACAGCAGGGCCACATAAGCACCAAGCAGTTGCGCTACGAGTCCAAGCCGACAAAGCCGTATTCTACAACTGCGAAATGCATGGCTACCAAGACACTCTCTACGTCCACACCATGCGTCAGTTCTACCGCGATTGCACCATCTCCGGCACCATCGACTTCATCTTCGGTGATGCAGCTGCCATCTTCCAAAGTTGCACCTTCCTAGTCCGTAAGCCCCTTCCAAACCAACAATGCATCGTAACGGCACACGGACGCAAAGAACGCCGCCAACCATCCGCCCTCATCATCCAAAACTGTTCGTTCAAGGCCGACACCGACCTGGCCCCAGTACAAAAACAGTTCCGATCCTTCCTTGGGCGACCATGGAAGGAATATTCGAGAACAATCATCATGGAATCATACATTGGTGATTTGATTCAACCAGAAGGGTGGTTGCCATGGGCTGGAGATTGGGGTTTGAAGACATGTTTTTACACCGAGTTCAACAATTATGGGCCAGGATCTGATAAGTCAAAGAGGGTGAAATGGAGAGGGATTAAGACCATTACGCCACAACATGCTTTGGATTTTACTGCCGGAAGGTTTGTTATGGGGGATAGATGGATTAAGCCCACCGGAGTTCCATATGTCTCCGGCATGACCAGGACCGGCGGAGCTGCTCATTGA

Protein sequence

MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKAIKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDPRTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDYPDWANPGMRRLLAAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGVYKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIKTITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAAH
Homology
BLAST of CaUC03G066080 vs. NCBI nr
Match: XP_038880188.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 555/586 (94.71%), Postives = 569/586 (97.10%), Query Frame = 0

Query: 1   MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKA 60
           M Y DDD+QNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQ ETS+PA G+K+HEISASVKA
Sbjct: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDP 120
           IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKE+EKDP
Sbjct: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
           ENTTGNAAEKMKKALKTSM+LSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGVY 300
           PDWANPGMRRLLAAGT+VKPNVVVAKDGSGQFRTIQEALDKVPKRKNNAT+VIHIK GVY
Sbjct: 241 PDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGVY 300

Query: 301 KEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           KEYL+IKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 KEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAAI 420
           AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDF+FGDAAA+
Sbjct: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQN SFKAD DLAPVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTE+NNYGPGSDKSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIKT 540

Query: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRT------GGAAH 581
           ITPQHA+DFTAGRF+ GD WIK TGVPYVSGMTRT      GGA H
Sbjct: 541 ITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTRAAAARGGATH 586

BLAST of CaUC03G066080 vs. NCBI nr
Match: XP_008450602.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo] >KAA0050920.1 putative pectinesterase/pectinesterase inhibitor 21 [Cucumis melo var. makuwa] >TYK10268.1 putative pectinesterase/pectinesterase inhibitor 21 [Cucumis melo var. makuwa])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 545/579 (94.13%), Postives = 570/579 (98.45%), Query Frame = 0

Query: 1   MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKA 60
           MGY+DDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETS+PATGNK+HEIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDP 120
           IKAICQPTDYKQEC+ASLKA GNNSSDP+ELVQAGFKAAMKLIQAAANKSV LK+LEKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           RTSKALAGCKELMDFA+DELKYSMNKLGEFDI+KLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
           +NTTGNAAEKMKKALKTSM+LSSNGLAMVSQ SSMLTELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGVY 300
           PDWANPGMRRLLAAG++VKPNVVVAKDGSGQFRTIQ ALD+VPKRKNNAT+VIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EYL IKK++THLMLIGDGPKKTIITG+KNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAAI 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCS KAD DLAPVQKQFRSFLGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTE+NNYGPGS+KSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 580
           ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 579

BLAST of CaUC03G066080 vs. NCBI nr
Match: XP_004135775.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus] >KGN66044.1 hypothetical protein Csa_007353 [Cucumis sativus])

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 538/578 (93.08%), Postives = 565/578 (97.75%), Query Frame = 0

Query: 1   MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKA 60
           MGY+DDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETS+PATGNK+HEIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDP 120
           IKAICQPTDYKQECVASLKA GNNSSDPKELVQAGFKAAMKLIQAAANKSV L +LEKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFA+DELKYSMNKLGEFDISKLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            NTTGNAAEKMKKALKTSM+LSSNGLAMVSQISSML+ELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGVY 300
           PDWANPGMRRLLAAG+KVKPNVVVAKDGSGQF+TIQEA+D+VPKRKNNAT+VIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EY+++KK+LTHLMLIGDGPKKTIITG+KNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAAI 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFK   DL PVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGL+TCFYTE+NNYGPGSDKSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540

Query: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGA 579
           ITPQHA+DFT GRF+ GDRWIKPTGVPYVSG+TRTGGA
Sbjct: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGA 578

BLAST of CaUC03G066080 vs. NCBI nr
Match: XP_022969472.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima])

HSP 1 Score: 981.9 bits (2537), Expect = 2.4e-282
Identity = 477/575 (82.96%), Postives = 531/575 (92.35%), Query Frame = 0

Query: 1   MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TS+PATG KAHEIS+S+KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDP 120
           IKAIC+PTDYK+ECV SLK  G+  SDPKELVQ GFKAAMKLIQAAA KS+TL+++EKDP
Sbjct: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R S+ALAGCKELMDF+VDELK+S+ KLG FDISKLDEMLIDIKIWLSA ITYQETCLDGF
Sbjct: 121 RASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            N+TG+A+EKMKKALK SMRLSSNGLAMV+++SSMLTELQIPGI+ RRLL IPVLGH+DY
Sbjct: 181 ANSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDY 240

Query: 241 PDWANPGMRRLLAAG-TKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGV 300
           P+WA PGM+RLLA    K KPN+VVAKDGSGQ++TIQEA++KVPKRKNNATFVIHIK GV
Sbjct: 241 PEWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           YKEY++I K LTHLMLIGDGPKKTII+G+KNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAA 420
           TAGPHKHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCTISGTIDF+FGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FK D DL PV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I+PEGWLPW GDWGL+TCFYTEFNNYGPGS KSKRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIK 540

Query: 541 TITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTR 575
           +IT QHA+DFT GRF++GDRWIK TG+PYVSGMT+
Sbjct: 541 SITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTK 572

BLAST of CaUC03G066080 vs. NCBI nr
Match: XP_022931680.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata])

HSP 1 Score: 979.9 bits (2532), Expect = 9.2e-282
Identity = 477/575 (82.96%), Postives = 531/575 (92.35%), Query Frame = 0

Query: 1   MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TS+PATG K HEIS+S+KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDP 120
           IKAIC+PTDYK+ECV SLK  G+  SDPKELVQ GFKAAMKLIQAAA KSVTL+++EKDP
Sbjct: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R S+ALA CKELMD++VDELK+S+ KLG FDISKLDEMLIDIKIWLSA ITYQETCLDGF
Sbjct: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            NTTG+A+EKMKKALKTSMRLSSNGLAMV+++SSMLTELQIPGI+ RRLL IPVLGH+D+
Sbjct: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDF 240

Query: 241 PDWANPGMRRLLAAG-TKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGV 300
           P+W +PGM+RLLA    K KPN+VVAKDGSGQ++TIQEA++KVPKRKNNATFVIHIK GV
Sbjct: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           YKEY+ I+K LTHLMLIGDGPKKTIITG+KNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAA 420
           TAGPHKHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCTISGTIDF+FGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FK D DL PV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGL+TCFYTEFNN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540

Query: 541 TITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTR 575
           +ITPQHA+DFT GRF++GDRWIK TGVPYVSGMT+
Sbjct: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 572

BLAST of CaUC03G066080 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 6.2e-188
Identity = 313/580 (53.97%), Postives = 432/580 (74.48%), Query Frame = 0

Query: 2   GYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKAI 61
           GY D+D++ KKRY II +SS+LL++MVVAVT+GV++N+ + +         EI+ SVKAI
Sbjct: 4   GYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD------EEITTSVKAI 63

Query: 62  KAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDPR 121
           K +C PTDYK+ C  +L+    ++SDP ELV+  F A MK I   A KS T+ EL+KDPR
Sbjct: 64  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 123

Query: 122 TSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFE 181
              AL  CKELMD+A+ EL  S  +LG+F+  K+DE L+ ++IWLSATI++++TCLDGF+
Sbjct: 124 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDYP 241
            T GNA E +KKALKT+++L+ NGLAMV+++S+ L ++QIP ++ RRLL        ++P
Sbjct: 184 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 243

Query: 242 DWANPGMRRLL-AAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGVY 301
            W +   RRLL A  ++VKP++VVA+DGSGQ++TI EAL+ VPK+K N TFV+HIK G+Y
Sbjct: 244 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKEGIY 303

Query: 302 KEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 361
           KEY+ + +S+THL+ IGDGP KT+I+GSK++ DG  T+KTATVA+  +HF+A++I FENT
Sbjct: 304 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 363

Query: 362 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAAI 421
           AG  KHQAVA+RV AD+++FYNC+  GYQDTLY H+ RQFYRDCTISGTIDF+FGDAAA+
Sbjct: 364 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 423

Query: 422 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGRP 481
           FQ+CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q C+   + D   V++Q +++LGRP
Sbjct: 424 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 483

Query: 482 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIKT 541
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+  +KRV W GIK 
Sbjct: 484 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 543

Query: 542 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAAH 581
           ++ +  L FT  +++ GD WI   GVPY+ G+    G+ +
Sbjct: 544 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGLFSGNGSTN 570

BLAST of CaUC03G066080 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 656.4 bits (1692), Expect = 3.1e-187
Identity = 316/581 (54.39%), Postives = 431/581 (74.18%), Query Frame = 0

Query: 2   GYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKAI 61
           GY DD+S+ K+RY +I +SS+LL++MVVAVTVGV+LN+ +           E++ASVKA+
Sbjct: 4   GY-DDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKA----EVNASVKAV 63

Query: 62  KAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDPR 121
           K +C PTDY++ C  +L   G N++DP ELV+  F   MK I  AA KS T+ EL+KD R
Sbjct: 64  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 123

Query: 122 TSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFE 181
           T  AL  CKELMD+A+DEL  S  +LG+F+   LDE LI+++IWLSA I+++ETCL+GF+
Sbjct: 124 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDYP 241
            T GNA E MKKALKT++ L+ NGLA++S++S+ + ++QIPG++ RRLL       + +P
Sbjct: 184 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 243

Query: 242 DWANPGMRRLL---AAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAG 301
            W +   R+LL   AA + VKP++VVA+DGSGQ++TI EAL  VPK K N TFV+HIKAG
Sbjct: 244 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 303

Query: 302 VYKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFE 361
           +YKEY+ + K+++HL+ IGDGP KTII+G+KN+ DG  T++TATVA+   +F+A++IGFE
Sbjct: 304 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 363

Query: 362 NTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAA 421
           NTAG  KHQAVA+RVQ+D+++F+NC   GYQDTLY H+ RQF+RDCTISGTIDF+FGDAA
Sbjct: 364 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 423

Query: 422 AIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLG 481
           A+FQ+CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q C+   + D   V++  +++LG
Sbjct: 424 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 483

Query: 482 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGI 541
           RPWKEYSRTIIM ++I D +QP+GW PW GD+GLKT FY+E  N GPGS  + RV W GI
Sbjct: 484 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 543

Query: 542 KTITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 580
           KT++ +  L FT  +++ GD WI   GVPY +G+     AA
Sbjct: 544 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPAA 572

BLAST of CaUC03G066080 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.1e-151
Identity = 273/575 (47.48%), Postives = 377/575 (65.57%), Query Frame = 0

Query: 6   DDSQNKKRYAIIGVSSMLLVAMVVAV--TVGVNLNQDETSEPATGNKAHEISASVKAIKA 65
           D    KK+  I GV + LLV MVVAV  T   N +  E   P       +I  +  A++A
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANK-SVTL-KELEKDPR 125
           +C PTDYK+ CV SL  A  +S+ P +L++ GF   ++ I+ +  K SV L  +   D  
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 TSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFE 185
           T  AL  C++LM+ A D+LK  ++    F I ++++ + D+++WLS +I YQ+TC+D FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGIS------RRRLLEIPVL 245
            T    ++ M+K  KTS  L+SNGLAM++ IS++L E  + G++       R+LL     
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS---- 243

Query: 246 GHDDYPDWANPGMRRLLAAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHI 305
             D  P W  P  RRL+A    VK NVVVA DGSGQ++TI EAL+ VPK  N   FVI+I
Sbjct: 244 AEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIYI 303

Query: 306 KAGVYKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDG-TPTFKTATVAVTAEHFMARD 365
           K GVY E + + K +TH+  IGDGP KT ITGS N+  G   T+ TATVA+  ++F A++
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 366 IGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIF 425
           IGFENTAGP  HQAVALRV AD AVFYNC++ GYQDTLYVH+ RQF+RDCT+SGT+DFIF
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 426 GDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFR 485
           GD   + Q+C  +VRKP+ +Q C++TA GR ++R+ + L++QNC    +    PV+   +
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 486 SFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVK 545
           ++LGRPWKE+SRTIIM + I D+I P GWLPW GD+ L T +Y E+ N GPGS++++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 546 WRGIKTITPQHALDFTAGRFVMGDRWIKPTGVPYV 570
           W GIK ++P+ AL FT  RF+ G+ WI P  VPY+
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of CaUC03G066080 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 511.1 bits (1315), Expect = 1.6e-143
Identity = 266/577 (46.10%), Postives = 369/577 (63.95%), Query Frame = 0

Query: 7   DSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKAIKAICQ 66
           D   KK++ + G  S  LV MVV+V V        TS+ +  +  + I  + KA++A+C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  PTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANK-SVTLK-ELEKDPRTSK 126
           PTD+K  CV SL  A  +S DP +L++ GFK  +K I  +  K S  +K + +K+P    
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFENTT 186
           A   C++LM  A+D+LK  M+    F + +++  + D+++WLS +I +Q+TC+D F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGIS------RRRLLEIPVLGHD 246
            N  + M K  KTS  LSSN LAMV++IS+++    + G++       R+LL       D
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----ED 243

Query: 247 DYPDWANPGMRRLLAA----GTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIH 306
             P W  P  RRL+AA       VK N VVA+DG+GQF+TI +AL+ VPK  N   F+IH
Sbjct: 244 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIH 303

Query: 307 IKAGVYKEYLIIKKSLTHLMLIGDGPKKTIITGSKNF-IDGTPTFKTATVAVTAEHFMAR 366
           IK G+YKE + + K + H+  IGDGP KT+ITGS NF I    TF TAT+ +  +HF A+
Sbjct: 304 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 363

Query: 367 DIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFI 426
           +IG ENTAGP   QAVALRV AD AVF++C++ G+QDTLYVH+ RQFYRDCT+SGT+DFI
Sbjct: 364 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 423

Query: 427 FGDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQF 486
           FGDA  I Q+C  +VRKP   Q C+VTA GR   R+ + L++  C    D    P++   
Sbjct: 424 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 483

Query: 487 RSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRV 546
           +++LGRPWKE+SRTIIM++ I D+I P GWLPW+GD+ LKT +Y E  N GPGS++++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 543

Query: 547 KWRGIKTITPQHALDFTAGRFVMGDRWIKPTGVPYVS 571
           KW GIK +TPQ AL +T  RF+ GD WI  T VPY +
Sbjct: 544 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of CaUC03G066080 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.7e-127
Identity = 262/702 (37.32%), Postives = 372/702 (52.99%), Query Frame = 0

Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKAIKAICQPTDYKQ 72
           +Y ++GV++ L++AMV+ V      +     E    ++  ++  +   +  IC  TDYKQ
Sbjct: 3   KYVLLGVTA-LIMAMVICVEANDGNSPSRKMEEV--HRESKLMITKTTVSIICASTDYKQ 62

Query: 73  ECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKS-VTLK-ELEKDPRTSKALAGCK 132
           +C  SL  A   S DP+ L+++ F  A+  I++  ++  + LK   + D  T +AL  C+
Sbjct: 63  DCTTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCR 122

Query: 133 ELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFENTTGNAAEK 192
           ELMD A+D+L+ + +K   F  ++L + + D+ +WLS +ITYQ+TC+DGFE     AA  
Sbjct: 123 ELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVM 182

Query: 193 MKKALKTSMRLSSNGLAMVSQISSMLTELQIP-GISRRRLLEIPVLG------------- 252
           M++ ++    L+SNGLA+ + +  +L   +IP    R R   + +LG             
Sbjct: 183 MERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQ 242

Query: 253 ------------------------------------------------------------ 312
                                                                       
Sbjct: 243 DSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSA 302

Query: 313 ------------------------------------------------------------ 372
                                                                       
Sbjct: 303 FSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLK 362

Query: 373 ------HDDYPDWANPGMRRLLAAGTK---VKPNVVVAKDGSGQFRTIQEALDKVPKRKN 432
                   ++P W  P  RRLLA   +   +K NVVVAKDGSG+ +TI +AL  VP  KN
Sbjct: 363 DRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-MKN 422

Query: 433 NATFVIHIKAGVYKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFI-DGTPTFKTATVAVT 492
              FVIHIK GVYKE + + K + H+M +GDGP KT+ITG   F+ D   T++TA+VAV 
Sbjct: 423 TKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVN 482

Query: 493 AEHFMARDIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTI 552
            ++FMA+DIGFENTAG  +HQAVALRV AD AVF+NC M+GYQDTLYVHT RQFYR+C +
Sbjct: 483 GDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRV 542

Query: 553 SGTIDFIFGDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDL 569
           SGTIDF+FGDA A+FQ+C F++R+P+ +QQCIVTA GRK+RR+ + ++I N     D   
Sbjct: 543 SGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASY 602

BLAST of CaUC03G066080 vs. ExPASy TrEMBL
Match: A0A5D3CGC3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180 PE=3 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 545/579 (94.13%), Postives = 570/579 (98.45%), Query Frame = 0

Query: 1   MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKA 60
           MGY+DDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETS+PATGNK+HEIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDP 120
           IKAICQPTDYKQEC+ASLKA GNNSSDP+ELVQAGFKAAMKLIQAAANKSV LK+LEKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           RTSKALAGCKELMDFA+DELKYSMNKLGEFDI+KLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
           +NTTGNAAEKMKKALKTSM+LSSNGLAMVSQ SSMLTELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGVY 300
           PDWANPGMRRLLAAG++VKPNVVVAKDGSGQFRTIQ ALD+VPKRKNNAT+VIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EYL IKK++THLMLIGDGPKKTIITG+KNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAAI 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCS KAD DLAPVQKQFRSFLGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTE+NNYGPGS+KSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 580
           ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 579

BLAST of CaUC03G066080 vs. ExPASy TrEMBL
Match: A0A1S3BPI8 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 545/579 (94.13%), Postives = 570/579 (98.45%), Query Frame = 0

Query: 1   MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKA 60
           MGY+DDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETS+PATGNK+HEIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDP 120
           IKAICQPTDYKQEC+ASLKA GNNSSDP+ELVQAGFKAAMKLIQAAANKSV LK+LEKDP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           RTSKALAGCKELMDFA+DELKYSMNKLGEFDI+KLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
           +NTTGNAAEKMKKALKTSM+LSSNGLAMVSQ SSMLTELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGVY 300
           PDWANPGMRRLLAAG++VKPNVVVAKDGSGQFRTIQ ALD+VPKRKNNAT+VIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EYL IKK++THLMLIGDGPKKTIITG+KNF+DGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAAI 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGRP 480
           FQ CTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCS KAD DLAPVQKQFRSFLGRP
Sbjct: 421 FQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTE+NNYGPGS+KSKRVKWRGIKT
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIKT 540

Query: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 580
           ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA
Sbjct: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 579

BLAST of CaUC03G066080 vs. ExPASy TrEMBL
Match: A0A0A0LYF5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1)

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 538/578 (93.08%), Postives = 565/578 (97.75%), Query Frame = 0

Query: 1   MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKA 60
           MGY+DDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETS+PATGNK+HEIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDP 120
           IKAICQPTDYKQECVASLKA GNNSSDPKELVQAGFKAAMKLIQAAANKSV L +LEKDP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R SKALAGCKELMDFA+DELKYSMNKLGEFDISKLDEMLIDI+IWLSATITYQETCLDGF
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            NTTGNAAEKMKKALKTSM+LSSNGLAMVSQISSML+ELQIPGISRRRLLEIPVLGHDDY
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIPVLGHDDY 240

Query: 241 PDWANPGMRRLLAAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGVY 300
           PDWANPGMRRLLAAG+KVKPNVVVAKDGSGQF+TIQEA+D+VPKRKNNAT+VIHIKAGVY
Sbjct: 241 PDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGVY 300

Query: 301 KEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360
           +EY+++KK+LTHLMLIGDGPKKTIITG+KNFIDGTPTFKTATVAVTAEHFMARDIGFENT
Sbjct: 301 QEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 360

Query: 361 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAAI 420
           AGP KHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCT+SGTIDFIFGDAAAI
Sbjct: 361 AGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAAI 420

Query: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGRP 480
           FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFK   DL PVQKQFRSFLGRP
Sbjct: 421 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGRP 480

Query: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIKT 540
           WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGL+TCFYTE+NNYGPGSDKSKRVKWRGIK 
Sbjct: 481 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIKN 540

Query: 541 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGA 579
           ITPQHA+DFT GRF+ GDRWIKPTGVPYVSG+TRTGGA
Sbjct: 541 ITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLTRTGGA 578

BLAST of CaUC03G066080 vs. ExPASy TrEMBL
Match: A0A6J1HWF1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1)

HSP 1 Score: 981.9 bits (2537), Expect = 1.2e-282
Identity = 477/575 (82.96%), Postives = 531/575 (92.35%), Query Frame = 0

Query: 1   MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TS+PATG KAHEIS+S+KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDP 120
           IKAIC+PTDYK+ECV SLK  G+  SDPKELVQ GFKAAMKLIQAAA KS+TL+++EKDP
Sbjct: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R S+ALAGCKELMDF+VDELK+S+ KLG FDISKLDEMLIDIKIWLSA ITYQETCLDGF
Sbjct: 121 RASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            N+TG+A+EKMKKALK SMRLSSNGLAMV+++SSMLTELQIPGI+ RRLL IPVLGH+DY
Sbjct: 181 ANSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDY 240

Query: 241 PDWANPGMRRLLAAG-TKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGV 300
           P+WA PGM+RLLA    K KPN+VVAKDGSGQ++TIQEA++KVPKRKNNATFVIHIK GV
Sbjct: 241 PEWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           YKEY++I K LTHLMLIGDGPKKTII+G+KNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAA 420
           TAGPHKHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCTISGTIDF+FGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FK D DL PV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I+PEGWLPW GDWGL+TCFYTEFNNYGPGS KSKRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIK 540

Query: 541 TITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTR 575
           +IT QHA+DFT GRF++GDRWIK TG+PYVSGMT+
Sbjct: 541 SITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTK 572

BLAST of CaUC03G066080 vs. ExPASy TrEMBL
Match: A0A6J1F058 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 4.5e-282
Identity = 477/575 (82.96%), Postives = 531/575 (92.35%), Query Frame = 0

Query: 1   MGYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKA 60
           M Y D  ++N+KRYAIIGVSSMLLVAMVVAVTVGVNLNQD TS+PATG K HEIS+S+KA
Sbjct: 1   MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKTHEISSSMKA 60

Query: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDP 120
           IKAIC+PTDYK+ECV SLK  G+  SDPKELVQ GFKAAMKLIQAAA KSVTL+++EKDP
Sbjct: 61  IKAICRPTDYKRECVDSLK--GSGKSDPKELVQVGFKAAMKLIQAAAKKSVTLRQIEKDP 120

Query: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180
           R S+ALA CKELMD++VDELK+S+ KLG FDISKLDEMLIDIKIWLSA ITYQETCLDGF
Sbjct: 121 RASQALAVCKELMDYSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGF 180

Query: 181 ENTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDY 240
            NTTG+A+EKMKKALKTSMRLSSNGLAMV+++SSMLTELQIPGI+ RRLL IPVLGH+D+
Sbjct: 181 ANTTGDASEKMKKALKTSMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDF 240

Query: 241 PDWANPGMRRLLAAG-TKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGV 300
           P+W +PGM+RLLA    K KPN+VVAKDGSGQ++TIQEA++KVPKRKNNATFVIHIK GV
Sbjct: 241 PEWVDPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGV 300

Query: 301 YKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360
           YKEY+ I+K LTHLMLIGDGPKKTIITG+KNF+DGT TFKTATVAV+AEHFMARDIGFEN
Sbjct: 301 YKEYVDIQKKLTHLMLIGDGPKKTIITGNKNFVDGTNTFKTATVAVSAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAA 420
           TAGPHKHQAVALRVQADK VF+NCEMHGYQDTLYVHTMRQFYRDCTISGTIDF+FGDAAA
Sbjct: 361 TAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGR 480
           +FQ+C+FLVRKPLPNQQ IVTAHGRKERRQPSALIIQN  FK D DL PV+KQFRS+LGR
Sbjct: 421 VFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGR 480

Query: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIK 540
           PWKEYSRTIIMESYI D+I PEGWLPWAGDWGL+TCFYTEFNN+GPGS K+KRVKWRGIK
Sbjct: 481 PWKEYSRTIIMESYIDDIIDPEGWLPWAGDWGLRTCFYTEFNNFGPGSGKAKRVKWRGIK 540

Query: 541 TITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTR 575
           +ITPQHA+DFT GRF++GDRWIK TGVPYVSGMT+
Sbjct: 541 SITPQHAVDFTPGRFILGDRWIKATGVPYVSGMTK 572

BLAST of CaUC03G066080 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 658.7 bits (1698), Expect = 4.4e-189
Identity = 313/580 (53.97%), Postives = 432/580 (74.48%), Query Frame = 0

Query: 2   GYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKAI 61
           GY D+D++ KKRY II +SS+LL++MVVAVT+GV++N+ + +         EI+ SVKAI
Sbjct: 4   GYDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD------EEITTSVKAI 63

Query: 62  KAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDPR 121
           K +C PTDYK+ C  +L+    ++SDP ELV+  F A MK I   A KS T+ EL+KDPR
Sbjct: 64  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 123

Query: 122 TSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFE 181
              AL  CKELMD+A+ EL  S  +LG+F+  K+DE L+ ++IWLSATI++++TCLDGF+
Sbjct: 124 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDYP 241
            T GNA E +KKALKT+++L+ NGLAMV+++S+ L ++QIP ++ RRLL        ++P
Sbjct: 184 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 243

Query: 242 DWANPGMRRLL-AAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAGVY 301
            W +   RRLL A  ++VKP++VVA+DGSGQ++TI EAL+ VPK+K N TFV+HIK G+Y
Sbjct: 244 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKEGIY 303

Query: 302 KEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFENT 361
           KEY+ + +S+THL+ IGDGP KT+I+GSK++ DG  T+KTATVA+  +HF+A++I FENT
Sbjct: 304 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 363

Query: 362 AGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAAAI 421
           AG  KHQAVA+RV AD+++FYNC+  GYQDTLY H+ RQFYRDCTISGTIDF+FGDAAA+
Sbjct: 364 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 423

Query: 422 FQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLGRP 481
           FQ+CT LVRKPL NQ C +TAHGRK+ R+ +  ++Q C+   + D   V++Q +++LGRP
Sbjct: 424 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 483

Query: 482 WKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGIKT 541
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+  +KRV W GIK 
Sbjct: 484 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 543

Query: 542 ITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAAH 581
           ++ +  L FT  +++ GD WI   GVPY+ G+    G+ +
Sbjct: 544 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGLFSGNGSTN 570

BLAST of CaUC03G066080 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 656.4 bits (1692), Expect = 2.2e-188
Identity = 316/581 (54.39%), Postives = 431/581 (74.18%), Query Frame = 0

Query: 2   GYRDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKAI 61
           GY DD+S+ K+RY +I +SS+LL++MVVAVTVGV+LN+ +           E++ASVKA+
Sbjct: 4   GY-DDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKA----EVNASVKAV 63

Query: 62  KAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKELEKDPR 121
           K +C PTDY++ C  +L   G N++DP ELV+  F   MK I  AA KS T+ EL+KD R
Sbjct: 64  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 123

Query: 122 TSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFE 181
           T  AL  CKELMD+A+DEL  S  +LG+F+   LDE LI+++IWLSA I+++ETCL+GF+
Sbjct: 124 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 183

Query: 182 NTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGISRRRLLEIPVLGHDDYP 241
            T GNA E MKKALKT++ L+ NGLA++S++S+ + ++QIPG++ RRLL       + +P
Sbjct: 184 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 243

Query: 242 DWANPGMRRLL---AAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHIKAG 301
            W +   R+LL   AA + VKP++VVA+DGSGQ++TI EAL  VPK K N TFV+HIKAG
Sbjct: 244 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 303

Query: 302 VYKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFE 361
           +YKEY+ + K+++HL+ IGDGP KTII+G+KN+ DG  T++TATVA+   +F+A++IGFE
Sbjct: 304 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 363

Query: 362 NTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIFGDAA 421
           NTAG  KHQAVA+RVQ+D+++F+NC   GYQDTLY H+ RQF+RDCTISGTIDF+FGDAA
Sbjct: 364 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 423

Query: 422 AIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFRSFLG 481
           A+FQ+CT LVRKPLPNQ C +TAHGRK+ R+ +  + Q C+   + D   V++  +++LG
Sbjct: 424 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 483

Query: 482 RPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVKWRGI 541
           RPWKEYSRTIIM ++I D +QP+GW PW GD+GLKT FY+E  N GPGS  + RV W GI
Sbjct: 484 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 543

Query: 542 KTITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMTRTGGAA 580
           KT++ +  L FT  +++ GD WI   GVPY +G+     AA
Sbjct: 544 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPAA 572

BLAST of CaUC03G066080 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 537.3 bits (1383), Expect = 1.5e-152
Identity = 273/575 (47.48%), Postives = 377/575 (65.57%), Query Frame = 0

Query: 6   DDSQNKKRYAIIGVSSMLLVAMVVAV--TVGVNLNQDETSEPATGNKAHEISASVKAIKA 65
           D    KK+  I GV + LLV MVVAV  T   N +  E   P       +I  +  A++A
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANK-SVTL-KELEKDPR 125
           +C PTDYK+ CV SL  A  +S+ P +L++ GF   ++ I+ +  K SV L  +   D  
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 TSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFE 185
           T  AL  C++LM+ A D+LK  ++    F I ++++ + D+++WLS +I YQ+TC+D FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGIS------RRRLLEIPVL 245
            T    ++ M+K  KTS  L+SNGLAM++ IS++L E  + G++       R+LL     
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS---- 243

Query: 246 GHDDYPDWANPGMRRLLAAGTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIHI 305
             D  P W  P  RRL+A    VK NVVVA DGSGQ++TI EAL+ VPK  N   FVI+I
Sbjct: 244 AEDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIYI 303

Query: 306 KAGVYKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFIDG-TPTFKTATVAVTAEHFMARD 365
           K GVY E + + K +TH+  IGDGP KT ITGS N+  G   T+ TATVA+  ++F A++
Sbjct: 304 KQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKN 363

Query: 366 IGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFIF 425
           IGFENTAGP  HQAVALRV AD AVFYNC++ GYQDTLYVH+ RQF+RDCT+SGT+DFIF
Sbjct: 364 IGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIF 423

Query: 426 GDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQFR 485
           GD   + Q+C  +VRKP+ +Q C++TA GR ++R+ + L++QNC    +    PV+   +
Sbjct: 424 GDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINK 483

Query: 486 SFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRVK 545
           ++LGRPWKE+SRTIIM + I D+I P GWLPW GD+ L T +Y E+ N GPGS++++RVK
Sbjct: 484 AYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVK 543

Query: 546 WRGIKTITPQHALDFTAGRFVMGDRWIKPTGVPYV 570
           W GIK ++P+ AL FT  RF+ G+ WI P  VPY+
Sbjct: 544 WPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPYM 567

BLAST of CaUC03G066080 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 511.1 bits (1315), Expect = 1.1e-144
Identity = 266/577 (46.10%), Postives = 369/577 (63.95%), Query Frame = 0

Query: 7   DSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKAIKAICQ 66
           D   KK++ + G  S  LV MVV+V V        TS+ +  +  + I  + KA++A+C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCA 63

Query: 67  PTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANK-SVTLK-ELEKDPRTSK 126
           PTD+K  CV SL  A  +S DP +L++ GFK  +K I  +  K S  +K + +K+P    
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFENTT 186
           A   C++LM  A+D+LK  M+    F + +++  + D+++WLS +I +Q+TC+D F    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GNAAEKMKKALKTSMRLSSNGLAMVSQISSMLTELQIPGIS------RRRLLEIPVLGHD 246
            N  + M K  KTS  LSSN LAMV++IS+++    + G++       R+LL       D
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----ED 243

Query: 247 DYPDWANPGMRRLLAA----GTKVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATFVIH 306
             P W  P  RRL+AA       VK N VVA+DG+GQF+TI +AL+ VPK  N   F+IH
Sbjct: 244 SIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIH 303

Query: 307 IKAGVYKEYLIIKKSLTHLMLIGDGPKKTIITGSKNF-IDGTPTFKTATVAVTAEHFMAR 366
           IK G+YKE + + K + H+  IGDGP KT+ITGS NF I    TF TAT+ +  +HF A+
Sbjct: 304 IKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAK 363

Query: 367 DIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFI 426
           +IG ENTAGP   QAVALRV AD AVF++C++ G+QDTLYVH+ RQFYRDCT+SGT+DFI
Sbjct: 364 NIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFI 423

Query: 427 FGDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDLAPVQKQF 486
           FGDA  I Q+C  +VRKP   Q C+VTA GR   R+ + L++  C    D    P++   
Sbjct: 424 FGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVN 483

Query: 487 RSFLGRPWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEFNNYGPGSDKSKRV 546
           +++LGRPWKE+SRTIIM++ I D+I P GWLPW+GD+ LKT +Y E  N GPGS++++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRV 543

Query: 547 KWRGIKTITPQHALDFTAGRFVMGDRWIKPTGVPYVS 571
           KW GIK +TPQ AL +T  RF+ GD WI  T VPY +
Sbjct: 544 KWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

BLAST of CaUC03G066080 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 457.2 bits (1175), Expect = 1.9e-128
Identity = 262/702 (37.32%), Postives = 372/702 (52.99%), Query Frame = 0

Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNQDETSEPATGNKAHEISASVKAIKAICQPTDYKQ 72
           +Y ++GV++ L++AMV+ V      +     E    ++  ++  +   +  IC  TDYKQ
Sbjct: 3   KYVLLGVTA-LIMAMVICVEANDGNSPSRKMEEV--HRESKLMITKTTVSIICASTDYKQ 62

Query: 73  ECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKS-VTLK-ELEKDPRTSKALAGCK 132
           +C  SL  A   S DP+ L+++ F  A+  I++  ++  + LK   + D  T +AL  C+
Sbjct: 63  DCTTSL--ATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCR 122

Query: 133 ELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGFENTTGNAAEK 192
           ELMD A+D+L+ + +K   F  ++L + + D+ +WLS +ITYQ+TC+DGFE     AA  
Sbjct: 123 ELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVM 182

Query: 193 MKKALKTSMRLSSNGLAMVSQISSMLTELQIP-GISRRRLLEIPVLG------------- 252
           M++ ++    L+SNGLA+ + +  +L   +IP    R R   + +LG             
Sbjct: 183 MERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQ 242

Query: 253 ------------------------------------------------------------ 312
                                                                       
Sbjct: 243 DSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSA 302

Query: 313 ------------------------------------------------------------ 372
                                                                       
Sbjct: 303 FSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLK 362

Query: 373 ------HDDYPDWANPGMRRLLAAGTK---VKPNVVVAKDGSGQFRTIQEALDKVPKRKN 432
                   ++P W  P  RRLLA   +   +K NVVVAKDGSG+ +TI +AL  VP  KN
Sbjct: 363 DRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-MKN 422

Query: 433 NATFVIHIKAGVYKEYLIIKKSLTHLMLIGDGPKKTIITGSKNFI-DGTPTFKTATVAVT 492
              FVIHIK GVYKE + + K + H+M +GDGP KT+ITG   F+ D   T++TA+VAV 
Sbjct: 423 TKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVN 482

Query: 493 AEHFMARDIGFENTAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTI 552
            ++FMA+DIGFENTAG  +HQAVALRV AD AVF+NC M+GYQDTLYVHT RQFYR+C +
Sbjct: 483 GDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRV 542

Query: 553 SGTIDFIFGDAAAIFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKADTDL 569
           SGTIDF+FGDA A+FQ+C F++R+P+ +QQCIVTA GRK+RR+ + ++I N     D   
Sbjct: 543 SGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASY 602

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880188.10.0e+0094.71probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida][more]
XP_008450602.10.0e+0094.13PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 [Cucumis melo] >K... [more]
XP_004135775.10.0e+0093.08putative pectinesterase/pectinesterase inhibitor 28 [Cucumis sativus] >KGN66044.... [more]
XP_022969472.12.4e-28282.96putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima][more]
XP_022931680.19.2e-28282.96putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q3E8Z86.2e-18853.97Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q8GX863.1e-18754.39Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q9FJ212.1e-15147.48Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Q8GXA11.6e-14346.10Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3... [more]
O234472.7e-12737.32Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5D3CGC30.0e+0094.13Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180... [more]
A0A1S3BPI80.0e+0094.13Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1[more]
A0A0A0LYF50.0e+0093.08Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1[more]
A0A6J1HWF11.2e-28282.96Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111468460 PE=3 SV=1[more]
A0A6J1F0584.5e-28282.96Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437837 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G27870.14.4e-18953.97Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.12.2e-18854.39Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G49180.11.5e-15247.48Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G06830.11.1e-14446.10Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G15980.11.9e-12837.32Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 56..208
e-value: 1.6E-36
score: 137.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 60..208
e-value: 2.6E-26
score: 92.7
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 54..212
e-value: 5.6E-23
score: 79.1
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 261..558
e-value: 6.4E-140
score: 465.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 255..572
e-value: 1.4E-120
score: 404.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 56..213
e-value: 7.4E-34
score: 118.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 59..213
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 16..573
NoneNo IPR availablePANTHERPTHR31707:SF131PECTINESTERASEcoord: 16..573
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 63..214
e-value: 2.26289E-44
score: 152.978
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 406..415
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 287..306
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 256..571

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC03G066080.1CaUC03G066080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity