Cla97C04G072970 (gene) Watermelon (97103) v2.5

Overview
NameCla97C04G072970
Typegene
OrganismCitrullus lanatus subsp. vulgaris cv. 97103 (Watermelon (97103) v2.5)
DescriptionMACPF domain-containing protein
LocationCla97Chr04: 20347837 .. 20351185 (-)
RNA-Seq ExpressionCla97C04G072970
SyntenyCla97C04G072970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGAGAGTGGAACTGCCTTGCATACTGCTATTAACGCTGTACAGGCACTTGGGAGAGGATTTGATGTAAATTTTGATACAAGGTTACTCTATTGTAAAGGCGTAGCTGGTTCTACTTTAGTTGAGGTTGATGTCGAACACTCTAGAGATATTTGTTTGTATGATGATGTAGTTTTGCTCAATGTTTCCTGGGACATCAAGAACTTCCAGGAGCCTATTGGGCATCGATCTTCTGGAATTTGTAGTTTCGATGAGGTAACTTGATGAATTCTCCATCTCTTTAGTCCCCTAAAATAGGAATTGTTTCATTTGTTGGCATTTTTTGTTAGTTTTATACTCAATTGTGATGGACCAAGATGATTTTGGAACCAGGAAGGAGATTTTAAGTCTGGATTGGCGCATATACAGAGTTAATTTGAACGACGAGTGCAACAAATTGATCAAAAGTTGATTATTTGCTTACTTAGTTTGATCGTGACTATAACAGGGTTGGAAAAATTCCCTCATGTGTCATATCTAGCTTTATCTTGCCCTGCTGTTAAGAAGTTGGCTAAGGTTGCGGCTGCATCCATTTTTTAGTTTGAATCTGGTGTTACAAAATCGTTGAAATTTGAAGTAACTCGTATAATACCCTTTACTCTATCTATGCCCAGTCACCTTTGTCATCTCTTCTAGACATAAACTACATTTTAAAGTAATAATTGAAGCTTTTCATCGGTGGTGTATTTAAAAAAGGGAACTTGATGCCTCAACCATAGGAACTTGCGAATAACTGATCTATGAAAGATTAGTGGACCCCACCCCCCCAATTGGTCAAGTAACTATTAAAAGTTTTGTCAAGAGTAACACCAAGAAGCTGGAAATTTAGTTGACAATCAGTCAGCAGATATCCATAGCATCTTCACGTAACTATGTATTAGCCTCAGAGCTATTGCAGCCGTACTGATCAATATATTCATAATCATATACTTGCAGATGGTAGATTATTTCAATCAAAAGGATGGTCTTTCTGAAGGTTACCCATCTGGTAGTTTTAATAGTGCATTCGGCTTCACTGGTTTTAAGCAGATTGACGCCGCAGCAACAAAGAGCCTTTCCACTGACGGATTTTATATTCCACTTGCAAAATTTCAGTTTACAAAAACCCCATTGGTGTTGCAAGAAAACGTCAAGCGGGCTGTTCCAACTACTTGGGATCCGCCATCCTTGGCAAGGTGGGAAGCCTCCACATTCTTGCAAGTGATAATGACGATAGAACTTCTTCCTTATCTTTAGTTTTTTTCTCTTTTCTGGCAGCTTTATTGAGAATTTTGGGACTCATGTAATCACATCTATAACCATTGGTGGGAAGGATGTGATATATATTAAACAGCACCAGTCTTCTCCCTTATCAACCATGGAGGTAAAGAGATATGTACAGGACATTGCAAACCAGAGGTTCTCTGACACGCAAGGTCAGACAGGTTCTGGAAGTATAAAACTTAAGGAAAAGGTTACTTACTAAAGTTTCATAACTCCTACAGTAAAATCCTTGGAACTCCGAGTTTTCTTTCTCTAATATCTAGTTTGCATTTAAATCCTACAGGGTCTAGATGGTGGATTATTCAACAACCAAGGGGTATATCCTCAGCCCACTTCTGCTCCATATCTCACTGGAAAAGAAGTATGTGGAAATTGTATTTTTATTCGGGGGACTGGTTTACACTCACTAGACATTCACAGTTGGTTTCACTTCTCATTTCGATCATTTTTCCATTAGGTGATATGGTTCAGCCATGTTATTCACTGTTAGAAACCATTGACATGATTGTTTGTCATTGTTTTGGTTGCACTCGTCACTCATGAATAATCATTTAATTGTGGATGAATACTTATTTCTTTTATTTTAATGAATTAGGATGTCACAGTCATATTCCGGAGGAGAGGAGGAGATGACTTGGAACAAAACCATGTCCGGTGGGCAAGAACTGTTAGGTCCTCCCCAGATGTTATTGAGATGACTTTTTACCCTATCACTGCTCTTCTTGACGGTGTAGCTGGAAAGGAGCATTTAACTCGAGCTATTCTTCTTTATCTTGAATGTAAGAATAACTTGTGCTCTTTACGTGGTGCTGCATTTGTAAACTTGTGTTGGTCCACCGGCATTCCCAAAAATTTGATCTGGACAATTTCCTTTTTGCAGACAAACCTCCATTAGAAGAGCTTAGATATTTTCTGGAGTTTCAGGTTCCTCGGGTATGGGCTCCTGTACGCAACACAATTCCCGGCCATCAAAGGAAAGAACCAGTATGCCCGTCTTTGCAATTCAGCATAATGGGCCAAAAGCTATATGTCAGCATAGAGCAGGTTCGAGCTTATTGTGGCTTTATTCACTTCAACAAATGTTAAGCAATTGATAACTTTTCAGAAATCGCACATAGTTAAAAAATTTGAATCGGGATTTTCATTATTTGGTCTCCATCTGAGCTCTTTTGCTTTCATTTAGCACTTATAAAGCGAAGAGAAAATAAACAGTCTTCTGATGTTAGAATCAGGATGTAGAGAAATGATTCCTAAAACCATGTTTGATAACTATTTCTGTTTCTTGTTTCTTGATAAAAAGACAGAAATGAAAATACTTCCCATGTTTGTTTCTTCTTTTGTTTTTTTCTTTTCTTTGGGTGAAACAATATTTTCCTGATAAGAGGATGTTGGGTCAATGTAAATGCAGATATCGATAGGACGGAAGCCAGTGACGGGTATGCGTTTGTCTCTTGAAGGGAGTAAGCAAAACCGGTTAAGTATCCATCTTCAACACCTAGCATCTCTTCCAAAAATTATGCACCCATACTGGGACACCCACGTTGCAATCGGTGCTCCCAAGTGGCAGGGGCCTGAGGAACAAGATAGCCGATGGTTCGAGCCTGTAAAGTGGAAAAACTTTTCGCACGTAAGCACTGCTCCTATCGAAAACCCTGAAACCTTCATGGGAGAGCTATCTGGTGTCTACATAGTCACTGGAGCTCAGCTTGGAGTCTGGGATTTCGGATCAAGAAACGTTTTGTACATGAAACTTCTGTATTCTAGACTGCCAGAGTGTACGATACGAAGAACATTATGGGATCATTTGCCGAACGACAAGTCGAGAAAACTAGTGTCCTCTATCGATAACAGGAATAATGCTGATGATTCGACTTCAGGTTCGAAAAACATACAAGGCAACAAACTGGCCAAGCTCGTCGACACAACGGAGATGAGCAAAGGGCCTCAAGATCCTCCAGGTCACTGGTTGGTTACGGGAGCAAAACTAGGTGTGGAAAAGGGTAAAATTGTGTTGAGAGTGAAGTATTCATTGCTTAACTATTGA

mRNA sequence

ATGGGGGAGAGTGGAACTGCCTTGCATACTGCTATTAACGCTGTACAGGCACTTGGGAGAGGATTTGATGTAAATTTTGATACAAGGTTACTCTATTGTAAAGGCGTAGCTGGTTCTACTTTAGTTGAGGTTGATGTCGAACACTCTAGAGATATTTGTTTGTATGATGATGTAGTTTTGCTCAATGTTTCCTGGGACATCAAGAACTTCCAGGAGCCTATTGGGCATCGATCTTCTGGAATTTGTAGTTTCGATGAGATGGTAGATTATTTCAATCAAAAGGATGGTCTTTCTGAAGGTTACCCATCTGGTAGTTTTAATAGTGCATTCGGCTTCACTGGTTTTAAGCAGATTGACGCCGCAGCAACAAAGAGCCTTTCCACTGACGGATTTTATATTCCACTTGCAAAATTTCAGTTTACAAAAACCCCATTGGTGTTGCAAGAAAACGTCAAGCGGGCTGTTCCAACTACTTGGGATCCGCCATCCTTGGCAAGCTTTATTGAGAATTTTGGGACTCATGTAATCACATCTATAACCATTGGTGGGAAGGATGTGATATATATTAAACAGCACCAGTCTTCTCCCTTATCAACCATGGAGGTAAAGAGATATGTACAGGACATTGCAAACCAGAGGTTCTCTGACACGCAAGGTCAGACAGGTTCTGGAAGTATAAAACTTAAGGAAAAGGGTCTAGATGGTGGATTATTCAACAACCAAGGGGTATATCCTCAGCCCACTTCTGCTCCATATCTCACTGGAAAAGAAGATGTCACAGTCATATTCCGGAGGAGAGGAGGAGATGACTTGGAACAAAACCATGTCCGGTGGGCAAGAACTGTTAGGTCCTCCCCAGATGTTATTGAGATGACTTTTTACCCTATCACTGCTCTTCTTGACGGTGTAGCTGGAAAGGAGCATTTAACTCGAGCTATTCTTCTTTATCTTGAATACAAACCTCCATTAGAAGAGCTTAGATATTTTCTGGAGTTTCAGGTTCCTCGGGTATGGGCTCCTGTACGCAACACAATTCCCGGCCATCAAAGGAAAGAACCAGTATGCCCGTCTTTGCAATTCAGCATAATGGGCCAAAAGCTATATGTCAGCATAGAGCAGATATCGATAGGACGGAAGCCAGTGACGGGTATGCGTTTGTCTCTTGAAGGGAGTAAGCAAAACCGGTTAAGTATCCATCTTCAACACCTAGCATCTCTTCCAAAAATTATGCACCCATACTGGGACACCCACGTTGCAATCGGTGCTCCCAAGTGGCAGGGGCCTGAGGAACAAGATAGCCGATGGTTCGAGCCTGTAAAGTGGAAAAACTTTTCGCACGTAAGCACTGCTCCTATCGAAAACCCTGAAACCTTCATGGGAGAGCTATCTGGTGTCTACATAGTCACTGGAGCTCAGCTTGGAGTCTGGGATTTCGGATCAAGAAACGTTTTGTACATGAAACTTCTGTATTCTAGACTGCCAGAGTGTACGATACGAAGAACATTATGGGATCATTTGCCGAACGACAAGTCGAGAAAACTAGTGTCCTCTATCGATAACAGGAATAATGCTGATGATTCGACTTCAGGTTCGAAAAACATACAAGGCAACAAACTGGCCAAGCTCGTCGACACAACGGAGATGAGCAAAGGGCCTCAAGATCCTCCAGGTCACTGGTTGGTTACGGGAGCAAAACTAGGTGTGGAAAAGGGTAAAATTGTGTTGAGAGTGAAGTATTCATTGCTTAACTATTGA

Coding sequence (CDS)

ATGGGGGAGAGTGGAACTGCCTTGCATACTGCTATTAACGCTGTACAGGCACTTGGGAGAGGATTTGATGTAAATTTTGATACAAGGTTACTCTATTGTAAAGGCGTAGCTGGTTCTACTTTAGTTGAGGTTGATGTCGAACACTCTAGAGATATTTGTTTGTATGATGATGTAGTTTTGCTCAATGTTTCCTGGGACATCAAGAACTTCCAGGAGCCTATTGGGCATCGATCTTCTGGAATTTGTAGTTTCGATGAGATGGTAGATTATTTCAATCAAAAGGATGGTCTTTCTGAAGGTTACCCATCTGGTAGTTTTAATAGTGCATTCGGCTTCACTGGTTTTAAGCAGATTGACGCCGCAGCAACAAAGAGCCTTTCCACTGACGGATTTTATATTCCACTTGCAAAATTTCAGTTTACAAAAACCCCATTGGTGTTGCAAGAAAACGTCAAGCGGGCTGTTCCAACTACTTGGGATCCGCCATCCTTGGCAAGCTTTATTGAGAATTTTGGGACTCATGTAATCACATCTATAACCATTGGTGGGAAGGATGTGATATATATTAAACAGCACCAGTCTTCTCCCTTATCAACCATGGAGGTAAAGAGATATGTACAGGACATTGCAAACCAGAGGTTCTCTGACACGCAAGGTCAGACAGGTTCTGGAAGTATAAAACTTAAGGAAAAGGGTCTAGATGGTGGATTATTCAACAACCAAGGGGTATATCCTCAGCCCACTTCTGCTCCATATCTCACTGGAAAAGAAGATGTCACAGTCATATTCCGGAGGAGAGGAGGAGATGACTTGGAACAAAACCATGTCCGGTGGGCAAGAACTGTTAGGTCCTCCCCAGATGTTATTGAGATGACTTTTTACCCTATCACTGCTCTTCTTGACGGTGTAGCTGGAAAGGAGCATTTAACTCGAGCTATTCTTCTTTATCTTGAATACAAACCTCCATTAGAAGAGCTTAGATATTTTCTGGAGTTTCAGGTTCCTCGGGTATGGGCTCCTGTACGCAACACAATTCCCGGCCATCAAAGGAAAGAACCAGTATGCCCGTCTTTGCAATTCAGCATAATGGGCCAAAAGCTATATGTCAGCATAGAGCAGATATCGATAGGACGGAAGCCAGTGACGGGTATGCGTTTGTCTCTTGAAGGGAGTAAGCAAAACCGGTTAAGTATCCATCTTCAACACCTAGCATCTCTTCCAAAAATTATGCACCCATACTGGGACACCCACGTTGCAATCGGTGCTCCCAAGTGGCAGGGGCCTGAGGAACAAGATAGCCGATGGTTCGAGCCTGTAAAGTGGAAAAACTTTTCGCACGTAAGCACTGCTCCTATCGAAAACCCTGAAACCTTCATGGGAGAGCTATCTGGTGTCTACATAGTCACTGGAGCTCAGCTTGGAGTCTGGGATTTCGGATCAAGAAACGTTTTGTACATGAAACTTCTGTATTCTAGACTGCCAGAGTGTACGATACGAAGAACATTATGGGATCATTTGCCGAACGACAAGTCGAGAAAACTAGTGTCCTCTATCGATAACAGGAATAATGCTGATGATTCGACTTCAGGTTCGAAAAACATACAAGGCAACAAACTGGCCAAGCTCGTCGACACAACGGAGATGAGCAAAGGGCCTCAAGATCCTCCAGGTCACTGGTTGGTTACGGGAGCAAAACTAGGTGTGGAAAAGGGTAAAATTGTGTTGAGAGTGAAGTATTCATTGCTTAACTATTGA

Protein sequence

MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVLLNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDAAATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRNVLYMKLLYSRLPECTIRRTLWDHLPNDKSRKLVSSIDNRNNADDSTSGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Homology
BLAST of Cla97C04G072970 vs. NCBI nr
Match: XP_038882143.1 (MACPF domain-containing protein CAD1-like [Benincasa hispida])

HSP 1 Score: 1141.3 bits (2951), Expect = 0.0e+00
Identity = 552/584 (94.52%), Postives = 570/584 (97.60%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVL 60
           MGESGTALHTA+NAVQALGRGFDVNFD+RLLYCKGV+GST+VE+D EHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTAMNAVQALGRGFDVNFDSRLLYCKGVSGSTIVEIDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120
           LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYIKQHQSSPLSTME+KRYVQDIANQRFSDTQGQ GSGSIK KEKGLDGGLFNN
Sbjct: 181 IGGKDVIYIKQHQSSPLSTMELKRYVQDIANQRFSDTQGQMGSGSIKHKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGG+DLEQNHVRWAR VRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGNDLEQNHVRWARNVRSSPDVIEMTFCPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYL+YKPPLEELRYFLEFQ+PRVWAP  +TIPGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLDYKPPLEELRYFLEFQIPRVWAPTCDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSH+STAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHISTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPNDKSRKLVSSIDNRNNADDSTSGSKNIQGNKLA 540
           RNVLYMKLLYSRLP CTIRR LWDHLPNDKS+KLV+S DN +  DDS+SG+K+IQGNKLA
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRMLWDHLPNDKSKKLVTSSDNSSKVDDSSSGTKSIQGNKLA 540

Query: 541 KLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           K+VDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 KVVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 584

BLAST of Cla97C04G072970 vs. NCBI nr
Match: XP_008463260.1 (PREDICTED: MACPF domain-containing protein CAD1-like [Cucumis melo] >TYK09776.1 MACPF domain-containing protein CAD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 551/586 (94.03%), Postives = 567/586 (96.76%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGST+VEVD EHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNSAFGFTG +Q DA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+EN+KRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQ+PRVWAP+ + +PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPN-DKSRKLVSSIDNRNNADDSTSGSKNIQG-NK 540
           RNVLYMKLLYSRLP CTIRRTLWDHLP  +KS+KLVSS DN NNAD+S+SG+K+ QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           LAKLVDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Cla97C04G072970 vs. NCBI nr
Match: XP_022144069.1 (MACPF domain-containing protein CAD1-like [Momordica charantia])

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 546/584 (93.49%), Postives = 567/584 (97.09%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVL 60
           MG++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GST+VEVD EH+RDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120
           LNVSWDIKNFQEPIGHRSSGICSFDEMV+YFNQKDGLSEGY SGSFNSAFGF G KQIDA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           A TK+LS DGFYIPLAKFQFTKTPLVLQENVKRA+PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF+PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQ+PRVWAPVR+T+PGHQRKEPVCPSLQF
Sbjct: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTHV I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPNDKSRKLVSSIDNRNNADDSTSGSKNIQGNKLA 540
           RNVLYMKLLYSRLP CTI+RTLWDHL NDKS+KLVSS DN +NADDS+SGS+NIQGNKLA
Sbjct: 481 RNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSKKLVSSSDN-SNADDSSSGSRNIQGNKLA 540

Query: 541 KLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           KLVDT+EMSKGP DPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 KLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of Cla97C04G072970 vs. NCBI nr
Match: XP_011653720.1 (MACPF domain-containing protein CAD1 [Cucumis sativus] >KGN54446.1 hypothetical protein Csa_011952 [Cucumis sativus])

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 546/586 (93.17%), Postives = 562/586 (95.90%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGST+VEVD EH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120
            NV WDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNS FGFTG KQ DA
Sbjct: 61  HNVPWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+ENVKRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQ+PRVWAP+ + IPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM PYWD H+AI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMVPYWDAHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPN-DKSRKLVSSIDNRNNADDSTSGSKNIQG-NK 540
           RNVLYMKLLYSRLP CTIRRTLWDHLP  +KS+KLVSS DN NNAD+S+S +KN QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           LAKLVDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Cla97C04G072970 vs. NCBI nr
Match: XP_022925277.1 (MACPF domain-containing protein CAD1-like [Cucurbita moschata])

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 540/585 (92.31%), Postives = 561/585 (95.90%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVL 60
           M ESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGST+VEVD EH+RDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120
           LNVSWDIKNFQ+PIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFN+AFGFTG KQIDA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           AA K+LSTDGFYIPLAKFQ T T L+L ENVKR+VPT+WDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300
           QGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTFYPITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQF 360
           DGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQ+PRVWAPVR+TIPGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKI+ PYWDTH+AI
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW FGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPNDKSRKLVSSIDNRN-NADDSTSGSKNIQGNKL 540
           RNVLYMKLLYSRLP CTIRRTLWDHLPNDKS+KLVSS DN N  ADDS+SGSKNIQGNKL
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNAAADDSSSGSKNIQGNKL 540

Query: 541 AKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           AKLVDTTEMSKGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 AKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Cla97C04G072970 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 3.8e-201
Identity = 342/582 (58.76%), Postives = 433/582 (74.40%), Query Frame = 0

Query: 4   SGTALHTAI-NAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVLLN 63
           S  AL T + NA+QALGRGFDV  D RLLYCKG  GS LV ++   +RD+ L    +L N
Sbjct: 13  SSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPN 72

Query: 64  VSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDAAA 123
           V  DI   +   G +   +CSF EM + FN + G+    P G FN+ F +TG  Q+DAA+
Sbjct: 73  VPADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAAS 132

Query: 124 TKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIG 183
           TKSL+  G++IPL   +  K  LVL   ++RAVP++WDP SLASFIEN+GTH++TS+TIG
Sbjct: 133 TKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIG 192

Query: 184 GKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQG 243
           G+DV+YI+QHQSSPL   E++ YV D+   RF + + Q+ +G +K K+K           
Sbjct: 193 GRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK----------- 252

Query: 244 VYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALLDG 303
                          D+TVIFRRRGGDDLEQ+H RWA TV ++PD+I MTF PI +LL+G
Sbjct: 253 ---------------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEG 312

Query: 304 VAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQFSI 363
           V G  HLTRAI LYLEYKPP+E+L+YFL++Q+ R WAP ++ +   QRKEPVC SLQFS+
Sbjct: 313 VPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQFSL 372

Query: 364 MGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAIGA 423
           MG KL++S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPKI+ P+WD+HV IGA
Sbjct: 373 MGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGA 432

Query: 424 PKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRN 483
           PKWQGPEEQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFGS+N
Sbjct: 433 PKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKN 492

Query: 484 VLYMKLLYSRLPECTIRRTLWDHLPNDKSRKLVSSIDNRNNADDSTSGSKNIQGNKLAKL 543
           VL++KLL+S++P CTIRR++WDH P   S +L     + +++ +  SG    Q  KLAK+
Sbjct: 493 VLHLKLLFSKVPGCTIRRSVWDHTPVASSGRLEPGGPSTSSSTEEVSG----QSGKLAKI 552

Query: 544 VDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           VD++EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 553 VDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Cla97C04G072970 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 3.7e-116
Identity = 250/600 (41.67%), Postives = 359/600 (59.83%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDIC---LYDDVVLLNVSWDIKNF 73
           A+ ++G G+D+  D RL YCKG +  + + +D++   D C   L   + + NVS  IK  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRL-LDIKEGDDNCEIVLPGGISIPNVSKSIKCD 73

Query: 74  QEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDAAATKSLSTDG 133
           +       S I  F +M + FNQ+  L+   PSG FN+ F F+   Q DAA TK+L+ DG
Sbjct: 74  KGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDG 133

Query: 134 FYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYIK 193
            +I L      K+ ++L+E+VK+AVP+TWDP +LA FI+ +GTH+I S+ +GGKDVIY K
Sbjct: 134 VFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAK 193

Query: 194 QHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLDGGLFNNQG 253
           Q  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L     ++ G
Sbjct: 194 QQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLG 253

Query: 254 VYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFYPITALLD 313
            Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+LL+
Sbjct: 254 SY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLN 313

Query: 314 GVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIP-GHQRKEPVCPSLQF 373
           GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAPV + +P G QRK+  C SLQF
Sbjct: 314 GVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQF 373

Query: 374 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 433
           S  G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPKI     D + +I
Sbjct: 374 SFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI 433

Query: 434 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 493
                   E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL V   G 
Sbjct: 434 ------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGF 493

Query: 494 RNVLYMKLLYSRLPECT-IRRTLWDHLPNDKSRK-LVSSI-----------------DNR 553
           +NVL+++L +SR+   T ++ + WD       +  L+S++                  N 
Sbjct: 494 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 553

Query: 554 NNADDSTSGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSL 582
           N+A          Q  KL K VDT+EM++GPQ+ PG+W+V+GA+L VEKGKI L+VKYSL
Sbjct: 554 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of Cla97C04G072970 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 9.2e-107
Identity = 239/601 (39.77%), Postives = 338/601 (56.24%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGVA-GSTLVEVDVEHSRDICLYDDVVLLNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS LVE+D   +RD+     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDAAATKSLSTD 130
            +       S I SF++M + FNQ   LS   PSG FN+ F F+     DA++ K+L+ D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYI 190
           G++I L   +  +  L L++ VKR VP++WD  +LA FIE +GTHV+  +T+GGKDVI++
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQPTS 250
           KQ + S     E+++ ++   ++RF     ++ S +     K  +  L    G+ P  TS
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLL-QWGLQPFGTS 254

Query: 251 APYL----TGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALLDGVAG 310
                   T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G+ G
Sbjct: 255 VSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPG 314

Query: 311 KEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIP-GHQRKEPVCPSLQFSIMG 370
              L+ A+ LYL YKPP+EEL  FLEFQ+PR WAPV   +P G +R +   PSLQFS+MG
Sbjct: 315 TGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMG 374

Query: 371 QKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMH-PYWDTHVAIGAP 430
            KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++ P  +H  + DT+  I  P
Sbjct: 375 PKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEP 434

Query: 431 KWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRNV 490
             +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G R V
Sbjct: 435 VEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKV 494

Query: 491 LYMKLLYSRLPECTIRRTLWDHL----------------------PNDKSRKLVSSIDNR 550
           L+++L +S       R++ WD+L                      PN  + K  S ID  
Sbjct: 495 LFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKID-I 554

Query: 551 NNADDSTSGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSL 583
           N+A      S  ++  KL  LVDT E+ +GP++ PG+W+VTGAKL VE GKI ++ KYSL
Sbjct: 555 NSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSL 603

BLAST of Cla97C04G072970 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.0e-97
Identity = 224/622 (36.01%), Postives = 331/622 (53.22%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----LVEVDVEHSRDICLYDDVVLLNVSWDIKN 73
           AV++LG+GFD+  D RL YCK   GS     LV +D   +R++ +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 74  FQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGF-TGFKQIDAAATKSLST 133
            +       S I  F++M +YFNQ+  ++   PSG+FN+ FGF +G    DAA  KSL  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 134 DGFYIPLAKFQF-TKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVI 193
           D   + L          L L + V+ AVP++WDP  LA FIE +GTHVIT +++GG+DV+
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 194 YIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQ------ 253
            ++Q +SS L    ++ ++ D+ +Q F        +GS  L  + L+    ++       
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLF--------TGSCLLSTRRLNKAYHHSHSQPKFP 251

Query: 254 ---GVYPQPTSAPY----LTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFY 313
               V+    +  +    +  +  +TVI  +RGGD   ++H  W  TV   PD I   F 
Sbjct: 252 EAFNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 311

Query: 314 PITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIP--GHQRKE 373
           PIT+LL  V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAPV N +P        
Sbjct: 312 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 371

Query: 374 PVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASL-PKIMH 433
              P+L  + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQHL +    +  
Sbjct: 372 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 431

Query: 434 PYWDTHVAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG----- 493
              D H+      W+G ++   + R+FEP+  K FSHV T P++    ++   S      
Sbjct: 432 KITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQN 491

Query: 494 --VYIVTGAQLGVWDFGSRNVLYMKLLYSRLPECTIRRTLWDHLPNDKSRK--LVSSI-- 553
              +IVTGAQL V   GS++VL+++L Y+++ +  + +  W H P   S+K  + SS+  
Sbjct: 492 DVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSM 551

Query: 554 --------------DNRNNADDS---TSGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLV 584
                         D      DS     G      NK+ K VD +++ +GPQ  PGHWLV
Sbjct: 552 PLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLV 611

BLAST of Cla97C04G072970 vs. ExPASy TrEMBL
Match: A0A5D3CHB3 (MACPF domain-containing protein CAD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00610 PE=4 SV=1)

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 551/586 (94.03%), Postives = 567/586 (96.76%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGST+VEVD EHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNSAFGFTG +Q DA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+EN+KRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQ+PRVWAP+ + +PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPN-DKSRKLVSSIDNRNNADDSTSGSKNIQG-NK 540
           RNVLYMKLLYSRLP CTIRRTLWDHLP  +KS+KLVSS DN NNAD+S+SG+K+ QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           LAKLVDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Cla97C04G072970 vs. ExPASy TrEMBL
Match: A0A1S3CJ73 (MACPF domain-containing protein CAD1-like OS=Cucumis melo OX=3656 GN=LOC103501463 PE=4 SV=1)

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 551/586 (94.03%), Postives = 567/586 (96.76%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGST+VEVD EHSRDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNSAFGFTG +Q DA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+EN+KRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQ+PRVWAP+ + +PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPN-DKSRKLVSSIDNRNNADDSTSGSKNIQG-NK 540
           RNVLYMKLLYSRLP CTIRRTLWDHLP  +KS+KLVSS DN NNAD+S+SG+K+ QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           LAKLVDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Cla97C04G072970 vs. ExPASy TrEMBL
Match: A0A6J1CR77 (MACPF domain-containing protein CAD1-like OS=Momordica charantia OX=3673 GN=LOC111013848 PE=4 SV=1)

HSP 1 Score: 1127.5 bits (2915), Expect = 0.0e+00
Identity = 546/584 (93.49%), Postives = 567/584 (97.09%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVL 60
           MG++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GST+VEVD EH+RDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120
           LNVSWDIKNFQEPIGHRSSGICSFDEMV+YFNQKDGLSEGY SGSFNSAFGF G KQIDA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           A TK+LS DGFYIPLAKFQFTKTPLVLQENVKRA+PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF+PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQ+PRVWAPVR+T+PGHQRKEPVCPSLQF
Sbjct: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTHV I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPNDKSRKLVSSIDNRNNADDSTSGSKNIQGNKLA 540
           RNVLYMKLLYSRLP CTI+RTLWDHL NDKS+KLVSS DN +NADDS+SGS+NIQGNKLA
Sbjct: 481 RNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSKKLVSSSDN-SNADDSSSGSRNIQGNKLA 540

Query: 541 KLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           KLVDT+EMSKGP DPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 KLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of Cla97C04G072970 vs. ExPASy TrEMBL
Match: A0A0A0L2V7 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G332620 PE=4 SV=1)

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 546/586 (93.17%), Postives = 562/586 (95.90%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVL 60
           MGESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGST+VEVD EH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120
            NV WDIKNFQEPIGHRSSGICSFDEM DYFNQKDGLSEGYPSGSFNS FGFTG KQ DA
Sbjct: 61  HNVPWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSVFGFTGLKQTDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           +ATKSLSTDGFYIPLAKFQFTKTPL L+ENVKRAVPTTWDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGL+GGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLEGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFNPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQF 360
           DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQ+PRVWAP+ + IPG QRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKIPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIM PYWD H+AI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMVPYWDAHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVY+VTGAQLGVWDFGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYVVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPN-DKSRKLVSSIDNRNNADDSTSGSKNIQG-NK 540
           RNVLYMKLLYSRLP CTIRRTLWDHLP  +KS+KLVSS DN NNAD+S+S +KN QG NK
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSDTKNTQGNNK 540

Query: 541 LAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           LAKLVDTTEMSKGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of Cla97C04G072970 vs. ExPASy TrEMBL
Match: A0A6J1EBB6 (MACPF domain-containing protein CAD1-like OS=Cucurbita moschata OX=3662 GN=LOC111432562 PE=4 SV=1)

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 540/585 (92.31%), Postives = 561/585 (95.90%), Query Frame = 0

Query: 1   MGESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVL 60
           M ESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGST+VEVD EH+RDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120
           LNVSWDIKNFQ+PIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFN+AFGFTG KQIDA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180
           AA K+LSTDGFYIPLAKFQ T T L+L ENVKR+VPT+WDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQ GSGS KLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300
           QGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVRSSPDVIEMTFYPITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQF 360
           DGVAGKEHLTRAI LYLEYKPPLEELRYFLEFQ+PRVWAPVR+TIPGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKI+ PYWDTH+AI
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW FGS
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPECTIRRTLWDHLPNDKSRKLVSSIDNRN-NADDSTSGSKNIQGNKL 540
           RNVLYMKLLYSRLP CTIRRTLWDHLPNDKS+KLVSS DN N  ADDS+SGSKNIQGNKL
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNAAADDSSSGSKNIQGNKL 540

Query: 541 AKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           AKLVDTTEMSKGPQD PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 AKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of Cla97C04G072970 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 702.6 bits (1812), Expect = 2.7e-202
Identity = 342/582 (58.76%), Postives = 433/582 (74.40%), Query Frame = 0

Query: 4   SGTALHTAI-NAVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDICLYDDVVLLN 63
           S  AL T + NA+QALGRGFDV  D RLLYCKG  GS LV ++   +RD+ L    +L N
Sbjct: 13  SSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPN 72

Query: 64  VSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDAAA 123
           V  DI   +   G +   +CSF EM + FN + G+    P G FN+ F +TG  Q+DAA+
Sbjct: 73  VPADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAAS 132

Query: 124 TKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIG 183
           TKSL+  G++IPL   +  K  LVL   ++RAVP++WDP SLASFIEN+GTH++TS+TIG
Sbjct: 133 TKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIG 192

Query: 184 GKDVIYIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQG 243
           G+DV+YI+QHQSSPL   E++ YV D+   RF + + Q+ +G +K K+K           
Sbjct: 193 GRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK----------- 252

Query: 244 VYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALLDG 303
                          D+TVIFRRRGGDDLEQ+H RWA TV ++PD+I MTF PI +LL+G
Sbjct: 253 ---------------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEG 312

Query: 304 VAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIPGHQRKEPVCPSLQFSI 363
           V G  HLTRAI LYLEYKPP+E+L+YFL++Q+ R WAP ++ +   QRKEPVC SLQFS+
Sbjct: 313 VPGLRHLTRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQFSL 372

Query: 364 MGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAIGA 423
           MG KL++S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPKI+ P+WD+HV IGA
Sbjct: 373 MGPKLFISADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGA 432

Query: 424 PKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRN 483
           PKWQGPEEQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFGS+N
Sbjct: 433 PKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKN 492

Query: 484 VLYMKLLYSRLPECTIRRTLWDHLPNDKSRKLVSSIDNRNNADDSTSGSKNIQGNKLAKL 543
           VL++KLL+S++P CTIRR++WDH P   S +L     + +++ +  SG    Q  KLAK+
Sbjct: 493 VLHLKLLFSKVPGCTIRRSVWDHTPVASSGRLEPGGPSTSSSTEEVSG----QSGKLAKI 552

Query: 544 VDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 585
           VD++EM KGPQD PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 553 VDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of Cla97C04G072970 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 420.2 bits (1079), Expect = 2.6e-117
Identity = 250/600 (41.67%), Postives = 359/600 (59.83%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDIC---LYDDVVLLNVSWDIKNF 73
           A+ ++G G+D+  D RL YCKG +  + + +D++   D C   L   + + NVS  IK  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRL-LDIKEGDDNCEIVLPGGISIPNVSKSIKCD 73

Query: 74  QEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDAAATKSLSTDG 133
           +       S I  F +M + FNQ+  L+   PSG FN+ F F+   Q DAA TK+L+ DG
Sbjct: 74  KGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDG 133

Query: 134 FYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYIK 193
            +I L      K+ ++L+E+VK+AVP+TWDP +LA FI+ +GTH+I S+ +GGKDVIY K
Sbjct: 134 VFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAK 193

Query: 194 QHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLDGGLFNNQG 253
           Q  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L     ++ G
Sbjct: 194 QQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLG 253

Query: 254 VYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFYPITALLD 313
            Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+LL+
Sbjct: 254 SY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLN 313

Query: 314 GVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIP-GHQRKEPVCPSLQF 373
           GV G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAPV + +P G QRK+  C SLQF
Sbjct: 314 GVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQF 373

Query: 374 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 433
           S  G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPKI     D + +I
Sbjct: 374 SFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI 433

Query: 434 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 493
                   E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL V   G 
Sbjct: 434 ------RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGF 493

Query: 494 RNVLYMKLLYSRLPECT-IRRTLWDHLPNDKSRK-LVSSI-----------------DNR 553
           +NVL+++L +SR+   T ++ + WD       +  L+S++                  N 
Sbjct: 494 KNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNI 553

Query: 554 NNADDSTSGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSL 582
           N+A          Q  KL K VDT+EM++GPQ+ PG+W+V+GA+L VEKGKI L+VKYSL
Sbjct: 554 NSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of Cla97C04G072970 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 389.0 bits (998), Expect = 6.5e-108
Identity = 239/601 (39.77%), Postives = 338/601 (56.24%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGVA-GSTLVEVDVEHSRDICLYDDVVLLNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS LVE+D   +RD+     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDAAATKSLSTD 130
            +       S I SF++M + FNQ   LS   PSG FN+ F F+     DA++ K+L+ D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYI 190
           G++I L   +  +  L L++ VKR VP++WD  +LA FIE +GTHV+  +T+GGKDVI++
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQPTS 250
           KQ + S     E+++ ++   ++RF     ++ S +     K  +  L    G+ P  TS
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLL-QWGLQPFGTS 254

Query: 251 APYL----TGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALLDGVAG 310
                   T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL G+ G
Sbjct: 255 VSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLSGLPG 314

Query: 311 KEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIP-GHQRKEPVCPSLQFSIMG 370
              L+ A+ LYL YKPP+EEL  FLEFQ+PR WAPV   +P G +R +   PSLQFS+MG
Sbjct: 315 TGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMG 374

Query: 371 QKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMH-PYWDTHVAIGAP 430
            KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++ P  +H  + DT+  I  P
Sbjct: 375 PKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEPIEEP 434

Query: 431 KWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGSRNV 490
             +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G R V
Sbjct: 435 VEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMGMRKV 494

Query: 491 LYMKLLYSRLPECTIRRTLWDHL----------------------PNDKSRKLVSSIDNR 550
           L+++L +S       R++ WD+L                      PN  + K  S ID  
Sbjct: 495 LFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSKID-I 554

Query: 551 NNADDSTSGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLVTGAKLGVEKGKIVLRVKYSL 583
           N+A      S  ++  KL  LVDT E+ +GP++ PG+W+VTGAKL VE GKI ++ KYSL
Sbjct: 555 NSAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSL 603

BLAST of Cla97C04G072970 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 359.0 bits (920), Expect = 7.2e-99
Identity = 224/622 (36.01%), Postives = 331/622 (53.22%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----LVEVDVEHSRDICLYDDVVLLNVSWDIKN 73
           AV++LG+GFD+  D RL YCK   GS     LV +D   +R++ +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADINC 71

Query: 74  FQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGF-TGFKQIDAAATKSLST 133
            +       S I  F++M +YFNQ+  ++   PSG+FN+ FGF +G    DAA  KSL  
Sbjct: 72  DKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSLGL 131

Query: 134 DGFYIPLAKFQF-TKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVI 193
           D   + L          L L + V+ AVP++WDP  LA FIE +GTHVIT +++GG+DV+
Sbjct: 132 DASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQDVV 191

Query: 194 YIKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNNQ------ 253
            ++Q +SS L    ++ ++ D+ +Q F        +GS  L  + L+    ++       
Sbjct: 192 VVRQDKSSDLDNDLLRHHLYDLGDQLF--------TGSCLLSTRRLNKAYHHSHSQPKFP 251

Query: 254 ---GVYPQPTSAPY----LTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFY 313
               V+    +  +    +  +  +TVI  +RGGD   ++H  W  TV   PD I   F 
Sbjct: 252 EAFNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFI 311

Query: 314 PITALLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQVPRVWAPVRNTIP--GHQRKE 373
           PIT+LL  V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAPV N +P        
Sbjct: 312 PITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMA 371

Query: 374 PVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASL-PKIMH 433
              P+L  + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQHL +    +  
Sbjct: 372 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 431

Query: 434 PYWDTHVAIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETFMGELSG----- 493
              D H+      W+G ++   + R+FEP+  K FSHV T P++    ++   S      
Sbjct: 432 KITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQN 491

Query: 494 --VYIVTGAQLGVWDFGSRNVLYMKLLYSRLPECTIRRTLWDHLPNDKSRK--LVSSI-- 553
              +IVTGAQL V   GS++VL+++L Y+++ +  + +  W H P   S+K  + SS+  
Sbjct: 492 DVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSM 551

Query: 554 --------------DNRNNADDS---TSGSKNIQGNKLAKLVDTTEMSKGPQDPPGHWLV 584
                         D      DS     G      NK+ K VD +++ +GPQ  PGHWLV
Sbjct: 552 PLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLV 611

BLAST of Cla97C04G072970 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 206.5 bits (524), Expect = 6.0e-53
Identity = 127/332 (38.25%), Postives = 191/332 (57.53%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGSTLVEVDVEHSRDIC---LYDDVVLLNVSWDIKNF 73
           A+ ++G G+D+  D RL YCKG +  + + +D++   D C   L   + + NVS  IK  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRL-LDIKEGDDNCEIVLPGGISIPNVSKSIKCD 73

Query: 74  QEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDAAATKSLSTDG 133
           +       S I  F +M + FNQ+  L+   PSG FN+ F F+   Q DAA TK+L+ DG
Sbjct: 74  KGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDG 133

Query: 134 FYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYIK 193
            +I L      K+ ++L+E+VK+AVP+TWDP +LA FI+ +GTH+I S+ +GGKDVIY K
Sbjct: 134 VFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAK 193

Query: 194 QHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGS--------IKLKEKGLDGGLFNNQG 253
           Q  SS L   ++++ ++++A++RF +      +GS        ++ KE+ L     ++ G
Sbjct: 194 QQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLG 253

Query: 254 VYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRSSPDVIEMTFYPITALLD 313
            Y           KED   + +RRGG+D     H  W +TV+  PDVI M+F PIT+LL+
Sbjct: 254 SY---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLN 313

Query: 314 GVAGKEHLTRAILLYLEYKP--PLEELRYFLE 332
           GV G   L+ AI LYL  KP   L  + +FLE
Sbjct: 314 GVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882143.10.0e+0094.52MACPF domain-containing protein CAD1-like [Benincasa hispida][more]
XP_008463260.10.0e+0094.03PREDICTED: MACPF domain-containing protein CAD1-like [Cucumis melo] >TYK09776.1 ... [more]
XP_022144069.10.0e+0093.49MACPF domain-containing protein CAD1-like [Momordica charantia][more]
XP_011653720.10.0e+0093.17MACPF domain-containing protein CAD1 [Cucumis sativus] >KGN54446.1 hypothetical ... [more]
XP_022925277.10.0e+0092.31MACPF domain-containing protein CAD1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9C7N23.8e-20158.76MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=... [more]
Q9STW53.7e-11641.67MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4... [more]
Q9SGN69.2e-10739.77MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=... [more]
Q8L6121.0e-9736.01MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A5D3CHB30.0e+0094.03MACPF domain-containing protein CAD1-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3CJ730.0e+0094.03MACPF domain-containing protein CAD1-like OS=Cucumis melo OX=3656 GN=LOC10350146... [more]
A0A6J1CR770.0e+0093.49MACPF domain-containing protein CAD1-like OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A0A0L2V70.0e+0093.17MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G332620 PE=4 ... [more]
A0A6J1EBB60.0e+0092.31MACPF domain-containing protein CAD1-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G29690.12.7e-20258.76MAC/Perforin domain-containing protein [more]
AT4G24290.22.6e-11741.67MAC/Perforin domain-containing protein [more]
AT1G28380.16.5e-10839.77MAC/Perforin domain-containing protein [more]
AT1G14780.17.2e-9936.01MAC/Perforin domain-containing protein [more]
AT4G24290.16.0e-5338.25MAC/Perforin domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (97103) v2.5
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020864Membrane attack complex component/perforin (MACPF) domainSMARTSM00457MACPF_8coord: 123..318
e-value: 1.1E-6
score: 38.2
IPR020864Membrane attack complex component/perforin (MACPF) domainPFAMPF01823MACPFcoord: 105..316
e-value: 2.5E-28
score: 99.6
IPR020864Membrane attack complex component/perforin (MACPF) domainPROSITEPS51412MACPF_2coord: 1..330
score: 20.004196
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 514..536
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 517..536
NoneNo IPR availablePANTHERPTHR33199:SF15MACPF DOMAIN-CONTAINING PROTEIN CAD1-LIKEcoord: 6..584
IPR044663MACPF domain-containing protein CAD1/NSL1-likePANTHERPTHR33199MACPF DOMAIN-CONTAINING PROTEIN CAD1coord: 6..584

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C04G072970.1Cla97C04G072970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009626 plant-type hypersensitive response
biological_process GO:2000031 regulation of salicylic acid mediated signaling pathway
biological_process GO:0006952 defense response
biological_process GO:0012501 programmed cell death