Sed0000177 (gene) Chayote v1

Overview
NameSed0000177
Typegene
OrganismSechium edule (Chayote v1)
DescriptionPectinesterase
LocationLG08: 38465075 .. 38467226 (-)
RNA-Seq ExpressionSed0000177
SyntenySed0000177
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTTCCTCCGACCTTACGGTAAGGTCGATGAAACCAACCAAATGTTCGAGGCTCGCCGGAGAACTCGAAAGAGGATTGCAGTCATTGTCTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTCTTGGTGTTGTTTTTGGGACTAGAAATGGTTCCAAAAGTGATGACAGGAACAATCAGAATGGTGCTCTTTCAACTTCCATCAAAGCAGTTTGTGATGTGACTTTGTACCCTGATACTTGTTACAATTCTTTTGCTCCTTTCGCTAACTCGAGCCACCTTGACCCGGGACAGATCTCCAAGCTTTCGGTTCAATTAGCGCTCGGCGAACTCTCTCAAGTAGCTAACTACATTTTTGAGCATGCCATTACCAATTCAACTGACGATAAGACCATATTGGCTCTGAGTAGTTGTCATGAACTTTTGGATTTGGCAATTGATCATCTCAATATCTCATTGTCTTCTAGTGATATTACATTGCTGAAAGCTGTGGATGATTTGAAAACATGGCTAACTTCTGCAGCTACTTACCAGCAGACTTGCATCGACGATCTCGAGGATGTCGACCCGTCGTTGGCGAACATGGTTGCGAGTTACTTGAAGAAATCCACTGAATTGACCAGCAATGGTCTTGCCATTGTGACCTTCTTTTCGAACCTTACTGATTCTTTCAGTCTACGGCGGTTGATGAGTTATGAAGATCACGAAAGCAATGGAGATTGGGCGCGCCCAATACTTCGAAAGCTAGCTCAGACTGATCTCAGGAAGCAGGTTAACATTGTTGTAGCGAAAGGGGGTTCGGGAAAGTACAAAACAATCGGGGCAGCTCTGAAGGCTGTTCCTGATAAGAGCAAGAAGAGAACTGTGATCTATGTAAAGAAGGGAATTTATAATGAGAATGTTGAAGTTGGAAAGAACAAATGGAATGTGGTGATGATTGGTGATGGAATGACTTCAACCACAGTGACTGGCAGACTCAACTTCATAGATGGGACTCCAACTTTTCAAAGTGCAACATTTGGTAAGAGAAAAACAGCTTTGTCTAAGCCTTTTAACTTGGTTTTCAAAGCTTAGAATCTAACCATTCATTCCTGTTTTGAAATAATCGCAAATACAATCAAGAATAAAAAACGTGTATTGTGTTAGCTACGTCTATGGAGAGATGAGAGAAACTGTCTTATTTAGGAGATAGAATACAAATGTTAGGTAAAGTGGATAAGAATCTTAAGAGAGTTTATATGAAGATTCTCTCATAAAACTCTAATACGACATCCGGCCCAAATTACATAAAAATCCAATATGAGATATAAAACACAATCCTAAGTCTTGGGAGCGTTACCCCCGAGCTCCCCACTAGATGCTTTGCCCTTGGACCCCAACTAGCTTGACCGGGTAGCGAAATCGCCCATACTGAGTACTCGTAATGCAACAGAGTTCAAGATATTTCAACAATTCTCATGTGGTTTTTTTTGCAGCTGCAAAGGGAAAAGGCTTTATTGCTATAGACATGGGATTCAAGAACACAGCTGGACCAAGCAAACACCAGGCTGTTGCCTTAATGTCGACGTCCGATCAATCAATCTTCTACCGTTGCCAAATGGATGCATACCAAGACACTCTCTATGCACACTCCAACCGCCAATTCTACCGTGATTGCAAGGTTTTCGGCACAGTCGATTTCATCTTCGGAAACTCAGCAGTGGTCCTACAAAACTGCATCATTGCTCCCAAGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCAGGTTTGATCCTAATCAAAACACTGGCATTTCACTACAAGGATCTACGATCCAACCATATGAGAACTTGAAAACCACCGAAACTTACCTCGGTCGACCATGGAAGAACTACTCGACAACCGTGTTTGTGCAGAGTAATTTGGGAAGCTTGATTCACCCAACTGGCTGGCTGCCTTGGGTTGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTTCAAAATAATGGCCCTGGTTCTTCAAAAGTTAAAAGAGTCAAATGGAAGGGAGTGAAGAACATTGACATTAAAACTGCCAAAAAGTTCACAGTTTCTTCTTTTATTCATGGTAAGGATTGGATCTCAAAAGCACAAGTCCCCTTCAAAGCCAGCCTTTGA

mRNA sequence

ATGTCTTTCCTCCGACCTTACGGTAAGGTCGATGAAACCAACCAAATGTTCGAGGCTCGCCGGAGAACTCGAAAGAGGATTGCAGTCATTGTCTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTCTTGGTGTTGTTTTTGGGACTAGAAATGGTTCCAAAAGTGATGACAGGAACAATCAGAATGGTGCTCTTTCAACTTCCATCAAAGCAGTTTGTGATGTGACTTTGTACCCTGATACTTGTTACAATTCTTTTGCTCCTTTCGCTAACTCGAGCCACCTTGACCCGGGACAGATCTCCAAGCTTTCGGTTCAATTAGCGCTCGGCGAACTCTCTCAAGTAGCTAACTACATTTTTGAGCATGCCATTACCAATTCAACTGACGATAAGACCATATTGGCTCTGAGTAGTTGTCATGAACTTTTGGATTTGGCAATTGATCATCTCAATATCTCATTGTCTTCTAGTGATATTACATTGCTGAAAGCTGTGGATGATTTGAAAACATGGCTAACTTCTGCAGCTACTTACCAGCAGACTTGCATCGACGATCTCGAGGATGTCGACCCGTCGTTGGCGAACATGGTTGCGAGTTACTTGAAGAAATCCACTGAATTGACCAGCAATGGTCTTGCCATTGTGACCTTCTTTTCGAACCTTACTGATTCTTTCAGTCTACGGCGGTTGATGAGTTATGAAGATCACGAAAGCAATGGAGATTGGGCGCGCCCAATACTTCGAAAGCTAGCTCAGACTGATCTCAGGAAGCAGGTTAACATTGTTGTAGCGAAAGGGGGTTCGGGAAAGTACAAAACAATCGGGGCAGCTCTGAAGGCTGTTCCTGATAAGAGCAAGAAGAGAACTGTGATCTATGTAAAGAAGGGAATTTATAATGAGAATGTTGAAGTTGGAAAGAACAAATGGAATGTGGTGATGATTGGTGATGGAATGACTTCAACCACAGTGACTGGCAGACTCAACTTCATAGATGGGACTCCAACTTTTCAAAGTGCAACATTTGCTGCAAAGGGAAAAGGCTTTATTGCTATAGACATGGGATTCAAGAACACAGCTGGACCAAGCAAACACCAGGCTGTTGCCTTAATGTCGACGTCCGATCAATCAATCTTCTACCGTTGCCAAATGGATGCATACCAAGACACTCTCTATGCACACTCCAACCGCCAATTCTACCGTGATTGCAAGGTTTTCGGCACAGTCGATTTCATCTTCGGAAACTCAGCAGTGGTCCTACAAAACTGCATCATTGCTCCCAAGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCAGGTTTGATCCTAATCAAAACACTGGCATTTCACTACAAGGATCTACGATCCAACCATATGAGAACTTGAAAACCACCGAAACTTACCTCGGTCGACCATGGAAGAACTACTCGACAACCGTGTTTGTGCAGAGTAATTTGGGAAGCTTGATTCACCCAACTGGCTGGCTGCCTTGGGTTGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTTCAAAATAATGGCCCTGGTTCTTCAAAAGTTAAAAGAGTCAAATGGAAGGGAGTGAAGAACATTGACATTAAAACTGCCAAAAAGTTCACAGTTTCTTCTTTTATTCATGGTAAGGATTGGATCTCAAAAGCACAAGTCCCCTTCAAAGCCAGCCTTTGA

Coding sequence (CDS)

ATGTCTTTCCTCCGACCTTACGGTAAGGTCGATGAAACCAACCAAATGTTCGAGGCTCGCCGGAGAACTCGAAAGAGGATTGCAGTCATTGTCTTGTCCTCTGTTGTTCTTGTTGGTGTTGTTCTTGGTGTTGTTTTTGGGACTAGAAATGGTTCCAAAAGTGATGACAGGAACAATCAGAATGGTGCTCTTTCAACTTCCATCAAAGCAGTTTGTGATGTGACTTTGTACCCTGATACTTGTTACAATTCTTTTGCTCCTTTCGCTAACTCGAGCCACCTTGACCCGGGACAGATCTCCAAGCTTTCGGTTCAATTAGCGCTCGGCGAACTCTCTCAAGTAGCTAACTACATTTTTGAGCATGCCATTACCAATTCAACTGACGATAAGACCATATTGGCTCTGAGTAGTTGTCATGAACTTTTGGATTTGGCAATTGATCATCTCAATATCTCATTGTCTTCTAGTGATATTACATTGCTGAAAGCTGTGGATGATTTGAAAACATGGCTAACTTCTGCAGCTACTTACCAGCAGACTTGCATCGACGATCTCGAGGATGTCGACCCGTCGTTGGCGAACATGGTTGCGAGTTACTTGAAGAAATCCACTGAATTGACCAGCAATGGTCTTGCCATTGTGACCTTCTTTTCGAACCTTACTGATTCTTTCAGTCTACGGCGGTTGATGAGTTATGAAGATCACGAAAGCAATGGAGATTGGGCGCGCCCAATACTTCGAAAGCTAGCTCAGACTGATCTCAGGAAGCAGGTTAACATTGTTGTAGCGAAAGGGGGTTCGGGAAAGTACAAAACAATCGGGGCAGCTCTGAAGGCTGTTCCTGATAAGAGCAAGAAGAGAACTGTGATCTATGTAAAGAAGGGAATTTATAATGAGAATGTTGAAGTTGGAAAGAACAAATGGAATGTGGTGATGATTGGTGATGGAATGACTTCAACCACAGTGACTGGCAGACTCAACTTCATAGATGGGACTCCAACTTTTCAAAGTGCAACATTTGCTGCAAAGGGAAAAGGCTTTATTGCTATAGACATGGGATTCAAGAACACAGCTGGACCAAGCAAACACCAGGCTGTTGCCTTAATGTCGACGTCCGATCAATCAATCTTCTACCGTTGCCAAATGGATGCATACCAAGACACTCTCTATGCACACTCCAACCGCCAATTCTACCGTGATTGCAAGGTTTTCGGCACAGTCGATTTCATCTTCGGAAACTCAGCAGTGGTCCTACAAAACTGCATCATTGCTCCCAAGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCAGGTTTGATCCTAATCAAAACACTGGCATTTCACTACAAGGATCTACGATCCAACCATATGAGAACTTGAAAACCACCGAAACTTACCTCGGTCGACCATGGAAGAACTACTCGACAACCGTGTTTGTGCAGAGTAATTTGGGAAGCTTGATTCACCCAACTGGCTGGCTGCCTTGGGTTGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTTCAAAATAATGGCCCTGGTTCTTCAAAAGTTAAAAGAGTCAAATGGAAGGGAGTGAAGAACATTGACATTAAAACTGCCAAAAAGTTCACAGTTTCTTCTTTTATTCATGGTAAGGATTGGATCTCAAAAGCACAAGTCCCCTTCAAAGCCAGCCTTTGA

Protein sequence

MSFLRPYGKVDETNQMFEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGSKSDDRNNQNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIFEHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQTCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESNGDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYNENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVLQNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYSTTVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKTAKKFTVSSFIHGKDWISKAQVPFKASL
Homology
BLAST of Sed0000177 vs. NCBI nr
Match: XP_038895610.1 (putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida])

HSP 1 Score: 971.1 bits (2509), Expect = 4.2e-279
Identity = 489/567 (86.24%), Postives = 527/567 (92.95%), Query Frame = 0

Query: 1   MSFLRPYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGS-KSDDRN 60
           MS LRPYGKVDET+Q+  +A RRTRKRIAVIVLSSVVLVGVV+ VVFGTRN S  +DD N
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYI 120
           NQ+GALSTSIKAVCDVTLYP TC N+F P ANSSHLDP QISKLSVQLALGELS+VA+YI
Sbjct: 61  NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADYI 120

Query: 121 FEHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
           F HAITNSTD+KTILAL +CHELLDLA+DHLNISLSSSDITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESN 240
           QTCID L +VDP+LAN++A+YLK STELTSNGLAIV+FFS LTDS SLRRLMSYEDH+SN
Sbjct: 181 QTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQSN 240

Query: 241 GDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYN 300
           GDWARPILRKLAQ D+RKQ +IVVAK  SGKYKTI AAL+AVP+KSKKRTVIYVKKG Y 
Sbjct: 241 GDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTA 360
           ENVEV KNKWNVV++GDGMT+T VTG LN IDGTPTF +ATFAAKGKGFIAIDMGF+NTA
Sbjct: 301 ENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVL 420
           GP+KHQAVALMSTSDQSIFYRCQMDAYQDTLY+HSNRQFYR+CKV+GTVDFIFGNSAVVL
Sbjct: 361 GPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQNTGIS+Q S+IQPYENLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNYS 480

Query: 481 TTVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKT 540
           TTVF+QSNLGSLIHP+GWLPWVGTSAPDTIFYAEFQN GPGSS  KRVKW GVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIKT 540

Query: 541 AKKFTVSSFIHGKDWISKAQVPFKASL 566
           AKKFTVSSFI GKDWISKAQVPFKAS+
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFKASI 567

BLAST of Sed0000177 vs. NCBI nr
Match: XP_022927225.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata])

HSP 1 Score: 959.9 bits (2480), Expect = 9.6e-276
Identity = 482/566 (85.16%), Postives = 521/566 (92.05%), Query Frame = 0

Query: 1   MSFLRPYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGSKSDDRNN 60
           MS LRPYGKVDE++QM  +A RRTRKR+ VIVLSSVVLVGVV+ VVFGTRN SK+D+ +N
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  QNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIF 120
            N ALSTSIKAVCD TLYPDTC N+F   ANSSHLDPGQI+K SVQLALGELS+VA+Y+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQ 180
           EHAITNSTD+KTILAL SCHELLDLAIDHLN SLSSS+IT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESNG 240
           TCID LE+VD +L N++A+YLK STE+TSNGLAIV+FFS LTDSFSLRRLMSYED  SNG
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYNE 300
           DWARPILRKLAQ DLRKQ +IVVAK  SGKYKTI  AL AVPDKSKKRTVIYVKKG+Y E
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTAG 360
           NVEV KNKWNVVMIGDGMTSTTVTGRLNFIDGTPTF +ATFAAKGKGFIAIDMGF+NTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQM+AYQDTLYAHSNRQFYRDCKV+GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYST 480
           NC IAPKLPLLGQKNTITAQGRFDPNQ+TGIS+Q S+IQP+ENLKTTETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKTA 540
           TVF+Q++ GSLIHPTGWLPWVGTSAPDTIFYAEFQN GPGSS   RVKWKG+KNID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFKASL 566
           KKFTVSSFIHGKDWISKAQVPF A+L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of Sed0000177 vs. NCBI nr
Match: KAG6583848.1 (putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 958.4 bits (2476), Expect = 2.8e-275
Identity = 481/566 (84.98%), Postives = 521/566 (92.05%), Query Frame = 0

Query: 1   MSFLRPYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGSKSDDRNN 60
           MS LRPYGKVDE++QM  +A RRTRKR+ VIVLSSVVLVGVV+ VVFGTRN SK+D+ +N
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  QNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIF 120
            N ALSTSIKAVCD TLYPDTC N+F   ANSSHLDPGQI+K SVQLALGELS+VA+Y+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQ 180
           EHAITNSTD+KTILAL SCHELLDLA+DHLN SLSSS+IT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAMDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESNG 240
           TCID LE+VD +L N++A+YLK STE+TSNGLAIV+FFS LTDSFSLRRLMSYED  SNG
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYNE 300
           DWARPILRKLAQ DLRKQ +IVVAK  SGKYKTI  AL AVPDKSKKRTVIYVKKG+Y E
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTAG 360
           NVEV KNKWNVVMIGDGMTSTTVTGRLNFIDGTPTF +ATFAAKGKGFIAIDMGF+NTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQM+AYQDTLYAHSNRQFYRDCKV+GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYST 480
           NC IAPKLPLLGQKNTITAQGRFDPNQ+TGIS+Q S+IQP+ENLKTTETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKTA 540
           TVF+Q++ GSLIHPTGWLPWVGTSAPDTIFYAEFQN GPGSS   RVKWKG+KNID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFKASL 566
           KKFTVSSFIHGKDWISKAQVPF A+L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of Sed0000177 vs. NCBI nr
Match: XP_004135547.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucumis sativus] >KGN65919.1 hypothetical protein Csa_023371 [Cucumis sativus])

HSP 1 Score: 956.8 bits (2472), Expect = 8.2e-275
Identity = 479/567 (84.48%), Postives = 523/567 (92.24%), Query Frame = 0

Query: 1   MSFLRPYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGS-KSDDRN 60
           MSFLRPYGKVDET+Q+  +ARRRTR+RI VIVLSSVVLV VV+ VVFGTRN S  +DD N
Sbjct: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYI 120
           NQNG+LSTSIKAVCDVTLYPDTC  +F P ANSSHLDPGQISKLSVQLALGELS+VA+YI
Sbjct: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 FEHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
           F+HAIT + D+KTILAL +CHELLDLA+DHLNISLSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESN 240
           QTCIDDL +VDP+LA++VA++LK STELTSNGLAIV+FFS LTDS SLRRLMSYE+H+SN
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYN 300
           GDWARPILRKLAQ+D+RK  +IVVAK  SGKYKTI  AL AVPDKSKKRTVIYVKKGIY 
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTA 360
           ENVEV KNKWNVVM+GDGMT+T V+G LN +DGTPTF +ATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+CKV+GTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQNTGIS+QGS IQP+ +LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480

Query: 481 TTVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKT 540
           TTVF+QSNLG LIHPTGWLPW+GTSAPDTIFYAEFQN GPGSS  KRVKWKGVKNID KT
Sbjct: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540

Query: 541 AKKFTVSSFIHGKDWISKAQVPFKASL 566
           AKKFTVSSFI GKDWISKAQVPF A+L
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of Sed0000177 vs. NCBI nr
Match: XP_023520176.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 953.0 bits (2462), Expect = 1.2e-273
Identity = 478/566 (84.45%), Postives = 519/566 (91.70%), Query Frame = 0

Query: 1   MSFLRPYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGSKSDDRNN 60
           MS LRPYGKVDE++QM  +A RRTRKR+ VIVLSSVVLVGVV+ VVFGTRN SK+D+ +N
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  QNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIF 120
            N ALSTSIKAVCD TLYPDTC N+F   ANSSHLDPGQI+K SVQLALGELS+VA+Y+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQ 180
           EHAITNSTD+KTILAL SCHELL+LAIDHLN SLSSS+IT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLELAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESNG 240
           TCID LE+VD +L N++A+YLK STE+TSNGLAIV+FFS LTDSFSLRRLMSY+D  SNG
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYDDRHSNG 240

Query: 241 DWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYNE 300
           DWARPILRKLAQ DLRKQ +IVVAK  SGKYKTI  AL AVPDKSKKRTVIYVKKG+Y E
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTAG 360
           NVEV KNKWNVVMIGDGMT TTVTGRLNFIDGTPTF +ATFAAKGKGFIAIDMGF+NTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTLTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQM+AYQDTLYAHSNRQFYRDCKV+GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYST 480
           NC IAPKLPLLGQKNTITAQGRFDPNQ+TGIS+Q S+IQP+ENLKTTETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKTA 540
           TVF+Q++ G LIHPTGWLPWVGTSAPDTIFYAEFQN GPGSS   RVKWKG+KNID KTA
Sbjct: 481 TVFMQNDFGRLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFKASL 566
           KKFTVSSFIHGKDWISKAQVPF A+L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of Sed0000177 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 3.8e-166
Identity = 307/569 (53.95%), Postives = 403/569 (70.83%), Query Frame = 0

Query: 6   PYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGSKSD---DRNNQN 65
           PYGKVDE   +  EARR+TRK IA+I +S V+L G+V+G VFGT    KS    + NN  
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  GALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIFEH 125
            ++S S+KAVCDVTL+ + C+ +     N+S L+P ++ + +V++ + E+S+  N     
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAIN----- 123

Query: 126 AITNST-DDKTILALSSCHELLDLAIDHLNISLSSS---DITLLKAVDDLKTWLTSAATY 185
           A ++S  D+K  + +++C ELLDL ID+LN +L+SS   D+T+ + VDDL+TWL+SA TY
Sbjct: 124 AFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTY 183

Query: 186 QQTCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSL-RRLMSYEDHE 245
           Q+TC++ L    P +     S+LK STELTSN LAI+T+   + DSF L RRL++  D E
Sbjct: 184 QRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVE 243

Query: 246 SNGDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGI 305
            +    R   R L  TDLRK  +IVVAK GSGKY+TI  AL+ VP+KS+KRT+IYVKKG+
Sbjct: 244 VDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGV 303

Query: 306 YNENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKN 365
           Y ENV+V K  WNV+++GDG + + V+GRLN IDGTPTF++ATFA  GKGF+A DMGF N
Sbjct: 304 YFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFIN 363

Query: 366 TAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAV 425
           TAGPSKHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYR+C + GTVDFIFGNSA 
Sbjct: 364 TAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSAS 423

Query: 426 VLQNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKN 485
           VLQ+C I P+ P+ GQ+NTITAQGR DPN NTGIS+    I P  +L    T+LGRPWKN
Sbjct: 424 VLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKN 483

Query: 486 YSTTVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDI 545
           +STTV + S L   I   GWLPW G SAPDTIFY E++N GPG+S   RVKWKG++ +  
Sbjct: 484 FSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLST 543

Query: 546 KTAKKFTVSSFIHGKDWISKAQVPFKASL 566
           K A +FTV  FI G  W+   +VPF++ L
Sbjct: 544 KEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of Sed0000177 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 5.3e-160
Identity = 297/568 (52.29%), Postives = 397/568 (69.89%), Query Frame = 0

Query: 7   YGKVDETNQ-MFEARRRTRKRIAVIVLSSVVLVGVVLGVVFGT--RNGSKSDDRNNQNGA 66
           YG++DE  Q   EA R+T+KRIA+I +SS+VLV +V+G V GT  R+ SK     N    
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIF--EH 126
           +S S+KA+CDVTL+ + C+ +     N+S   P ++ K +V++ + ELS+V +     EH
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEH 123

Query: 127 AITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQTC 186
                 D+ T  A+ +C EL+ LA+D LN +++SS    LK  DDL+TWL+S  TYQ+TC
Sbjct: 124 -----MDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETC 183

Query: 187 IDDLEDVD-PSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESNGD 246
           +D L + + PSL     ++LK STE+TSN LAI+T+   + D+   RR    E   +   
Sbjct: 184 MDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVV 243

Query: 247 WA-RPIL---RKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGI 306
            A  P++   R L   DL+K+  IVVAK GSGKY+TIG AL  V +K++K T+IYVKKG+
Sbjct: 244 VADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGV 303

Query: 307 YNENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKN 366
           Y ENV V K KWNVVM+GDG + T V+  LNFIDGTPTF++ATFA  GKGF+A DMGF N
Sbjct: 304 YLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFIN 363

Query: 367 TAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAV 426
           TAGP+KHQAVALM ++D S+FY+C MDA+QDT+YAH+ RQFYRDC + GTVDFIFGN+AV
Sbjct: 364 TAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAV 423

Query: 427 VLQNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKN 486
           V Q C I P+ P+ GQ+NTITAQGR DPNQNTGIS+   TI+P +NL   +T+LGRPWK+
Sbjct: 424 VFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKD 483

Query: 487 YSTTVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVK-NID 546
           +STTV ++S +   I+P GWLPW G +APDTIFYAE+ N+GPG+S   RVKW+G+K ++ 
Sbjct: 484 FSTTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLT 543

Query: 547 IKTAKKFTVSSFIHGKDWISKAQVPFKA 564
            K A KFTV  FI G +W+   +VPF +
Sbjct: 544 KKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of Sed0000177 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 4.5e-135
Identity = 270/582 (46.39%), Postives = 371/582 (63.75%), Query Frame = 0

Query: 7   YGKVDETNQMFEARRRTRKRIAVIVLSSVVLVGVVL----GVVFGTRNGS---KSDDRNN 66
           YGKV+E  Q     ++TRKR+ +I +SS+VL+ V++    GVV   RN      SD    
Sbjct: 10  YGKVNELEQQ-AYEKKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVPQ 69

Query: 67  QNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIF 126
              + + S+KAVCD T YP +C++S +    S+  DP  + KLS+++A+ ELS   + + 
Sbjct: 70  TELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKLR 129

Query: 127 EHAITNSTDDKTILALSSCHELLDLAIDHLNISLS-----SSDITLLKAVDDLKTWLTSA 186
            +A     D +   A+  C  +   A+D LN S+S     +  I    +V +++TWL++A
Sbjct: 130 ANA---EQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSAA 189

Query: 187 ATYQQTCIDDLEDVDPSLA----NMVASYLKKSTELTSNGLAIVTFFSNLTDSFSL---- 246
            T Q TC+D + +++ + A      + + ++ STE  SN LAIVT    L   F      
Sbjct: 190 LTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIHH 249

Query: 247 RRLMSYEDHESNGDWARPILRKLAQTDLRKQV-NIVVAKGGSGKYKTIGAALKAVPDKSK 306
           RRL+ +       +W     R+L +        + VVAK GSG++KTIG ALK V  KS+
Sbjct: 250 RRLLGFP------EWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKSE 309

Query: 307 KRTVIYVKKGIYNENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGK 366
           +R  +YVK+G Y EN+++ KN WNV++ GDG   T V G  NF+DGTPTF++ATFA KGK
Sbjct: 310 ERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKGK 369

Query: 367 GFIAIDMGFKNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFG 426
           GFIA D+GF N AG SKHQAVAL S SD+S+F+RC  D +QDTLYAHSNRQFYRDC + G
Sbjct: 370 GFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITG 429

Query: 427 TVDFIFGNSAVVLQNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKT 486
           T+DFIFGN+AVV Q+C I P+ PL  Q NTITAQG+ DPNQNTGI +Q STI P+ N  T
Sbjct: 430 TIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNLT 489

Query: 487 TETYLGRPWKNYSTTVFVQSNLGSLIHPTGWLPWV-GTSAPDTIFYAEFQNNGPGSSKVK 546
             TYLGRPWK++STTV +QS++G+L++P GW+ WV     P TIFYAE+QN+GPG+   +
Sbjct: 490 APTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVSQ 549

Query: 547 RVKWKGVK-NIDIKTAKKFTVSSFIHGKDWISKAQVPFKASL 566
           RVKW G K  I  + A++FTV SFI G +W+  A V F ++L
Sbjct: 550 RVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

BLAST of Sed0000177 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.5e-122
Identity = 250/573 (43.63%), Postives = 363/573 (63.35%), Query Frame = 0

Query: 7   YGKVDETNQMFEARRRTRKRIAVIVLSSVVLVGVVLGVVFGT---RNGSKSDDRNNQNGA 66
           YGKVDE  Q    +++TRKR+ ++ +S VVL+ V++  V  T   +N ++S         
Sbjct: 10  YGKVDEA-QDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPELT 69

Query: 67  LSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIFEHAI 126
            STS+KA+C VT +P++C +S +   +S+  DP  + KLS+++ + EL  +++      +
Sbjct: 70  PSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD--LPEKL 129

Query: 127 TNSTDDKTI-LALSSCHELLDLAIDHLNISLSSSDITLLK------AVDDLKTWLTSAAT 186
           +  T+D+ I  AL  C +L++ A+D LN ++S+ D    K       ++DLKTWL++  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIDDLEDV--------DPSLANMVASYLKKSTELTSNGLAIVT-FFSNLTD----S 246
             +TC D L+++        + ++   + S + +STE TSN LAIV+   S L+D     
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 FSLRRLMSYEDHESNGDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDK 306
              RRLMS+  H+ + D+ +   R+L QT   K  ++ VA  G+G   T+  A+  VP K
Sbjct: 250 HRRRRLMSHH-HQQSVDFEKWARRRLLQTAGLKP-DVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SKKRTVIYVKKGIYNENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAK 366
           S K  VIYVK G Y ENV + K+KWNV++ GDG   T ++G  NF+DGTPT+++ATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFKNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKV 426
           GKGFI  D+G  NTAG +KHQAVA  S SD S++Y+C  D +QDTLY HSNRQFYRDC V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 FGTVDFIFGNSAVVLQNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENL 486
            GT+DFIFG++AVV Q C I P+ PL  Q NTITAQG+ DPNQ++G+S+Q  TI    N+
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNV 489

Query: 487 KTTETYLGRPWKNYSTTVFVQSNLGSLIHPTGWLPWV-GTSAPDTIFYAEFQNNGPGSSK 546
               TYLGRPWK +STTV +++ +G+++ P+GW+ WV G   P +I Y E++N GPGS  
Sbjct: 490 -IAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 VKRVKWKGVKNI-DIKTAKKFTVSSFIHGKDWI 555
            +RVKW G K +     A KFTV++ +HG DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

BLAST of Sed0000177 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 6.2e-108
Identity = 227/556 (40.83%), Postives = 321/556 (57.73%), Query Frame = 0

Query: 27  IAVIVLSSVVLVGVVLGVVFGTRNGSKSDDRNNQNGALSTSIKAVCDVTLYPDTCYNSFA 86
           IA ++ + +VL+ V +G+               Q    +T+++AVC  T Y +TC NS  
Sbjct: 14  IAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSLM 73

Query: 87  PFANSSHLDPGQISKLSVQLALGELSQVANYIFEHAITNSTDDK-TILALSSCHELLDLA 146
             A+     P  + KL   + +  +              + +DK T  AL  C +L++ A
Sbjct: 74  K-ASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMNDA 133

Query: 147 IDHLNISLSSSD----ITLLKAVDDLKTWLTSAATYQQTCIDDLEDVDPSLANMVASYLK 206
            D L   L + D      +   V+DL+ WL+ +  YQQTC+D  E+ +  L+  +    K
Sbjct: 134 TDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFK 193

Query: 207 KSTELTSNGLAIVTFFSNLTDSFSL-----------RRLMSYEDHESNGDWARPILRKLA 266
            S ELTSNGLA++T  SNL   F++           R+L+S ED      W  P  R+L 
Sbjct: 194 TSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED--GIPSWVGPNTRRLM 253

Query: 267 QTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYNENVEVGKNKWNV 326
            T    + N+VVA  GSG+YKTI  AL AVP  ++K  VIY+K+G+YNE V+V K   +V
Sbjct: 254 ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKMTHV 313

Query: 327 VMIGDGMTSTTVTGRLNFIDG-TPTFQSATFAAKGKGFIAIDMGFKNTAGPSKHQAVALM 386
             IGDG T T +TG LN+  G   T+ +AT A  G  F A ++GF+NTAGP  HQAVAL 
Sbjct: 314 TFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAVALR 373

Query: 387 STSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVLQNCIIAPKLPL 446
            ++D ++FY CQ+D YQDTLY HS+RQF+RDC V GTVDFIFG+  VVLQNC I  + P+
Sbjct: 374 VSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVRKPM 433

Query: 447 LGQKNTITAQGRFDPNQNTGISLQGSTI---QPYENLKT-TETYLGRPWKNYSTTVFVQS 506
             Q   ITAQGR D  ++TG+ LQ   I     Y  +K+  + YLGRPWK +S T+ + +
Sbjct: 434 KSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTIIMGT 493

Query: 507 NLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKTAKKFTVS 562
            +  +I P GWLPW G  A +T++YAE++NNGPGS++ +RVKW G+K +  K A +FT +
Sbjct: 494 TIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPA 553

BLAST of Sed0000177 vs. ExPASy TrEMBL
Match: A0A6J1ENB0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1)

HSP 1 Score: 959.9 bits (2480), Expect = 4.7e-276
Identity = 482/566 (85.16%), Postives = 521/566 (92.05%), Query Frame = 0

Query: 1   MSFLRPYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGSKSDDRNN 60
           MS LRPYGKVDE++QM  +A RRTRKR+ VIVLSSVVLVGVV+ VVFGTRN SK+D+ +N
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  QNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIF 120
            N ALSTSIKAVCD TLYPDTC N+F   ANSSHLDPGQI+K SVQLALGELS+VA+Y+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADYLL 120

Query: 121 EHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQ 180
           EHAITNSTD+KTILAL SCHELLDLAIDHLN SLSSS+IT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESNG 240
           TCID LE+VD +L N++A+YLK STE+TSNGLAIV+FFS LTDSFSLRRLMSYED  SNG
Sbjct: 181 TCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYNE 300
           DWARPILRKLAQ DLRKQ +IVVAK  SGKYKTI  AL AVPDKSKKRTVIYVKKG+Y E
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTAG 360
           NVEV KNKWNVVMIGDGMTSTTVTGRLNFIDGTPTF +ATFAAKGKGFIAIDMGF+NTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQM+AYQDTLYAHSNRQFYRDCKV+GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYST 480
           NC IAPKLPLLGQKNTITAQGRFDPNQ+TGIS+Q S+IQP+ENLKTTETYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNYST 480

Query: 481 TVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKTA 540
           TVF+Q++ GSLIHPTGWLPWVGTSAPDTIFYAEFQN GPGSS   RVKWKG+KNID KTA
Sbjct: 481 TVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFKASL 566
           KKFTVSSFIHGKDWISKAQVPF A+L
Sbjct: 541 KKFTVSSFIHGKDWISKAQVPFNATL 566

BLAST of Sed0000177 vs. ExPASy TrEMBL
Match: A0A0A0LY08 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G538170 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.0e-275
Identity = 479/567 (84.48%), Postives = 523/567 (92.24%), Query Frame = 0

Query: 1   MSFLRPYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGS-KSDDRN 60
           MSFLRPYGKVDET+Q+  +ARRRTR+RI VIVLSSVVLV VV+ VVFGTRN S  +DD N
Sbjct: 1   MSFLRPYGKVDETDQLRLDARRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYI 120
           NQNG+LSTSIKAVCDVTLYPDTC  +F P ANSSHLDPGQISKLSVQLALGELS+VA+YI
Sbjct: 61  NQNGSLSTSIKAVCDVTLYPDTCQKAFGPIANSSHLDPGQISKLSVQLALGELSKVADYI 120

Query: 121 FEHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
           F+HAIT + D+KTILAL +CHELLDLA+DHLNISLSSSDITLLKAVDDLKTW+TSAATYQ
Sbjct: 121 FDHAITKTADNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWITSAATYQ 180

Query: 181 QTCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESN 240
           QTCIDDL +VDP+LA++VA++LK STELTSNGLAIV+FFS LTDS SLRRLMSYE+H+SN
Sbjct: 181 QTCIDDLAEVDPALADLVANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYENHQSN 240

Query: 241 GDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYN 300
           GDWARPILRKLAQ+D+RK  +IVVAK  SGKYKTI  AL AVPDKSKKRTVIYVKKGIY 
Sbjct: 241 GDWARPILRKLAQSDIRKHADIVVAKDRSGKYKTITEALGAVPDKSKKRTVIYVKKGIYK 300

Query: 301 ENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTA 360
           ENVEV KNKWNVVM+GDGMT+T V+G LN +DGTPTF +ATFAAKGKGFIAIDMGF NTA
Sbjct: 301 ENVEVVKNKWNVVMVGDGMTATIVSGNLNVVDGTPTFSTATFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+CKV+GTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCEMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQNTGIS+QGS IQP+ +LKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFNDLKTTETYLGRPWKNYS 480

Query: 481 TTVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKT 540
           TTVF+QSNLG LIHPTGWLPW+GTSAPDTIFYAEFQN GPGSS  KRVKWKGVKNID KT
Sbjct: 481 TTVFMQSNLGRLIHPTGWLPWIGTSAPDTIFYAEFQNYGPGSSTSKRVKWKGVKNIDTKT 540

Query: 541 AKKFTVSSFIHGKDWISKAQVPFKASL 566
           AKKFTVSSFI GKDWISKAQVPF A+L
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of Sed0000177 vs. ExPASy TrEMBL
Match: A0A6J1KL23 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 7.5e-274
Identity = 478/566 (84.45%), Postives = 519/566 (91.70%), Query Frame = 0

Query: 1   MSFLRPYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGSKSDDRNN 60
           MS LRPYGKVDE++QM  +A RRTRKR+ VIVLSSVVLVGVV+ VVFGTRN SK+D+ +N
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKNDESDN 60

Query: 61  QNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIF 120
            N ALSTSIKAVCD TLYPDTC N+F P ANSSHLDPGQI+K SVQLAL ELS+VA+Y+ 
Sbjct: 61  HNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADYLL 120

Query: 121 EHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQ 180
           EHAITNSTD+KTILAL  CHELLDLA+DHLN SLSSS+IT+LKAVDDLKTWL+SAATYQQ
Sbjct: 121 EHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATYQQ 180

Query: 181 TCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESNG 240
           TCID LE+VD +L N++A+YLK STE+TSNGLAIV+FFS LTDSFSLRRLMSYED  SNG
Sbjct: 181 TCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHSNG 240

Query: 241 DWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYNE 300
           DWARPILRKLAQ DLRKQ +IVVAK  SGKYKTI  ALKAV DKSKKRTVIYVKKG+Y E
Sbjct: 241 DWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVYKE 300

Query: 301 NVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTAG 360
           NVEV KNKWNVVMIGDGMTSTTVTG+LNFIDGTPTF +ATFAAKGKGFIAIDMGF+NTAG
Sbjct: 301 NVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENTAG 360

Query: 361 PSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVLQ 420
           PSKHQAVALMSTSDQSIFYRCQM+AYQDTLYAHSNRQFYRDCKV+GTVDFIFGNSAVVLQ
Sbjct: 361 PSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVVLQ 420

Query: 421 NCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYST 480
           NC IAPKLPLLGQKNTITAQGRFDPNQ+TGIS+Q S+IQP+ENLKTT+TYLGRPWKNYST
Sbjct: 421 NCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNYST 480

Query: 481 TVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKTA 540
           TVF+Q+N GSLIHPTGWLPWVGTSAPDTIFYAEFQN GPGSS   RVKWKG+KNID KTA
Sbjct: 481 TVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTKTA 540

Query: 541 KKFTVSSFIHGKDWISKAQVPFKASL 566
           KKFTVSSFI GKDWISKAQVPF A+L
Sbjct: 541 KKFTVSSFIDGKDWISKAQVPFNATL 566

BLAST of Sed0000177 vs. ExPASy TrEMBL
Match: A0A5A7VDG1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G00770 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 7.5e-274
Identity = 479/567 (84.48%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 1   MSFLRPYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGS-KSDDRN 60
           MSFLRPYGKVDET+Q+  +A RRTR+RI VIVLSSVVLV VV+ VVFGTRN S  +DD N
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYI 120
           NQNG+LS SIKAVCDVTLYPDTC  +F PFANSSHLDPGQISKL+VQLALGELS+VA++I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FEHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
           F+HAITN+TDDKTILAL +CHELLDLA+DHLNI+LSSSDITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESN 240
           QTCID L +VDP+LA++ A++LK STELTSNGLAIV+FFS LTDS SLRRLMSYED +SN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYN 300
           GDWARPILRKLAQ+D+RKQ +IVVAK  SGKYKTI  ALKAVPDKSKKRTVIYVKKG Y 
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTA 360
           E VEV KNKWNVVM+GDGM +T V+G LN IDGTPTF +A+FAAKGKGFIAIDMGF NTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCQM+AYQDTLYAHSNRQFYR+CKV+GTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQNTGIS+QGS IQP++NLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKT 540
           TTVF+QSNLGSLIHP+GWLPWVGTSAPDTIFYAEFQN G GSS  KRVKWKGVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIHGKDWISKAQVPFKASL 566
           AKKFTVSSFI GKDWISKAQVPF A+L
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of Sed0000177 vs. ExPASy TrEMBL
Match: A0A1S3BDA4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 7.5e-274
Identity = 479/567 (84.48%), Postives = 522/567 (92.06%), Query Frame = 0

Query: 1   MSFLRPYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGS-KSDDRN 60
           MSFLRPYGKVDET+Q+  +A RRTR+RI VIVLSSVVLV VV+ VVFGTRN S  +DD N
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  NQNGALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYI 120
           NQNG+LS SIKAVCDVTLYPDTC  +F PFANSSHLDPGQISKL+VQLALGELS+VA++I
Sbjct: 61  NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADHI 120

Query: 121 FEHAITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQ 180
           F+HAITN+TDDKTILAL +CHELLDLA+DHLNI+LSSSDITLLKAVDDLKTWLTSAATYQ
Sbjct: 121 FDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATYQ 180

Query: 181 QTCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESN 240
           QTCID L +VDP+LA++ A++LK STELTSNGLAIV+FFS LTDS SLRRLMSYED +SN
Sbjct: 181 QTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQSN 240

Query: 241 GDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYN 300
           GDWARPILRKLAQ+D+RKQ +IVVAK  SGKYKTI  ALKAVPDKSKKRTVIYVKKG Y 
Sbjct: 241 GDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTYK 300

Query: 301 ENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKNTA 360
           E VEV KNKWNVVM+GDGM +T V+G LN IDGTPTF +A+FAAKGKGFIAIDMGF NTA
Sbjct: 301 EKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINTA 360

Query: 361 GPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVL 420
           GPSKHQAVALMSTSDQSIFYRCQM+AYQDTLYAHSNRQFYR+CKV+GTVDFIFGNSAVVL
Sbjct: 361 GPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVVL 420

Query: 421 QNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKNYS 480
           QNC IAP+LPLLGQKNTITAQGRFDPNQNTGIS+QGS IQP++NLKTTETYLGRPWKNYS
Sbjct: 421 QNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNYS 480

Query: 481 TTVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKT 540
           TTVF+QSNLGSLIHP+GWLPWVGTSAPDTIFYAEFQN G GSS  KRVKWKGVKNIDIKT
Sbjct: 481 TTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIKT 540

Query: 541 AKKFTVSSFIHGKDWISKAQVPFKASL 566
           AKKFTVSSFI GKDWISKAQVPF A+L
Sbjct: 541 AKKFTVSSFIDGKDWISKAQVPFTATL 567

BLAST of Sed0000177 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 586.3 bits (1510), Expect = 2.7e-167
Identity = 307/569 (53.95%), Postives = 403/569 (70.83%), Query Frame = 0

Query: 6   PYGKVDETNQM-FEARRRTRKRIAVIVLSSVVLVGVVLGVVFGTRNGSKSD---DRNNQN 65
           PYGKVDE   +  EARR+TRK IA+I +S V+L G+V+G VFGT    KS    + NN  
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  GALSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIFEH 125
            ++S S+KAVCDVTL+ + C+ +     N+S L+P ++ + +V++ + E+S+  N     
Sbjct: 64  DSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAIN----- 123

Query: 126 AITNST-DDKTILALSSCHELLDLAIDHLNISLSSS---DITLLKAVDDLKTWLTSAATY 185
           A ++S  D+K  + +++C ELLDL ID+LN +L+SS   D+T+ + VDDL+TWL+SA TY
Sbjct: 124 AFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGTY 183

Query: 186 QQTCIDDLEDVDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSL-RRLMSYEDHE 245
           Q+TC++ L    P +     S+LK STELTSN LAI+T+   + DSF L RRL++  D E
Sbjct: 184 QRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADVE 243

Query: 246 SNGDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGI 305
            +    R   R L  TDLRK  +IVVAK GSGKY+TI  AL+ VP+KS+KRT+IYVKKG+
Sbjct: 244 VDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGV 303

Query: 306 YNENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKN 365
           Y ENV+V K  WNV+++GDG + + V+GRLN IDGTPTF++ATFA  GKGF+A DMGF N
Sbjct: 304 YFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFIN 363

Query: 366 TAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAV 425
           TAGPSKHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYR+C + GTVDFIFGNSA 
Sbjct: 364 TAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSAS 423

Query: 426 VLQNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKN 485
           VLQ+C I P+ P+ GQ+NTITAQGR DPN NTGIS+    I P  +L    T+LGRPWKN
Sbjct: 424 VLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKN 483

Query: 486 YSTTVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDI 545
           +STTV + S L   I   GWLPW G SAPDTIFY E++N GPG+S   RVKWKG++ +  
Sbjct: 484 FSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLST 543

Query: 546 KTAKKFTVSSFIHGKDWISKAQVPFKASL 566
           K A +FTV  FI G  W+   +VPF++ L
Sbjct: 544 KEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of Sed0000177 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 565.8 bits (1457), Expect = 3.8e-161
Identity = 297/568 (52.29%), Postives = 397/568 (69.89%), Query Frame = 0

Query: 7   YGKVDETNQ-MFEARRRTRKRIAVIVLSSVVLVGVVLGVVFGT--RNGSKSDDRNNQNGA 66
           YG++DE  Q   EA R+T+KRIA+I +SS+VLV +V+G V GT  R+ SK     N    
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIF--EH 126
           +S S+KA+CDVTL+ + C+ +     N+S   P ++ K +V++ + ELS+V +     EH
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEH 123

Query: 127 AITNSTDDKTILALSSCHELLDLAIDHLNISLSSSDITLLKAVDDLKTWLTSAATYQQTC 186
                 D+ T  A+ +C EL+ LA+D LN +++SS    LK  DDL+TWL+S  TYQ+TC
Sbjct: 124 -----MDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETC 183

Query: 187 IDDLEDVD-PSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSFSLRRLMSYEDHESNGD 246
           +D L + + PSL     ++LK STE+TSN LAI+T+   + D+   RR    E   +   
Sbjct: 184 MDALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVV 243

Query: 247 WA-RPIL---RKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGI 306
            A  P++   R L   DL+K+  IVVAK GSGKY+TIG AL  V +K++K T+IYVKKG+
Sbjct: 244 VADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGV 303

Query: 307 YNENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAKGKGFIAIDMGFKN 366
           Y ENV V K KWNVVM+GDG + T V+  LNFIDGTPTF++ATFA  GKGF+A DMGF N
Sbjct: 304 YLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFIN 363

Query: 367 TAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAV 426
           TAGP+KHQAVALM ++D S+FY+C MDA+QDT+YAH+ RQFYRDC + GTVDFIFGN+AV
Sbjct: 364 TAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAV 423

Query: 427 VLQNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENLKTTETYLGRPWKN 486
           V Q C I P+ P+ GQ+NTITAQGR DPNQNTGIS+   TI+P +NL   +T+LGRPWK+
Sbjct: 424 VFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKD 483

Query: 487 YSTTVFVQSNLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVK-NID 546
           +STTV ++S +   I+P GWLPW G +APDTIFYAE+ N+GPG+S   RVKW+G+K ++ 
Sbjct: 484 FSTTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLT 543

Query: 547 IKTAKKFTVSSFIHGKDWISKAQVPFKA 564
            K A KFTV  FI G +W+   +VPF +
Sbjct: 544 KKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of Sed0000177 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 441.4 bits (1134), Expect = 1.1e-123
Identity = 250/573 (43.63%), Postives = 363/573 (63.35%), Query Frame = 0

Query: 7   YGKVDETNQMFEARRRTRKRIAVIVLSSVVLVGVVLGVVFGT---RNGSKSDDRNNQNGA 66
           YGKVDE  Q    +++TRKR+ ++ +S VVL+ V++  V  T   +N ++S         
Sbjct: 10  YGKVDEA-QDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPELT 69

Query: 67  LSTSIKAVCDVTLYPDTCYNSFAPFANSSHLDPGQISKLSVQLALGELSQVANYIFEHAI 126
            STS+KA+C VT +P++C +S +   +S+  DP  + KLS+++ + EL  +++      +
Sbjct: 70  PSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISD--LPEKL 129

Query: 127 TNSTDDKTI-LALSSCHELLDLAIDHLNISLSSSDITLLK------AVDDLKTWLTSAAT 186
           +  T+D+ I  AL  C +L++ A+D LN ++S+ D    K       ++DLKTWL++  T
Sbjct: 130 SKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIDDLEDV--------DPSLANMVASYLKKSTELTSNGLAIVT-FFSNLTD----S 246
             +TC D L+++        + ++   + S + +STE TSN LAIV+   S L+D     
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 FSLRRLMSYEDHESNGDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDK 306
              RRLMS+  H+ + D+ +   R+L QT   K  ++ VA  G+G   T+  A+  VP K
Sbjct: 250 HRRRRLMSHH-HQQSVDFEKWARRRLLQTAGLKP-DVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SKKRTVIYVKKGIYNENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATFAAK 366
           S K  VIYVK G Y ENV + K+KWNV++ GDG   T ++G  NF+DGTPT+++ATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFKNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKV 426
           GKGFI  D+G  NTAG +KHQAVA  S SD S++Y+C  D +QDTLY HSNRQFYRDC V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 FGTVDFIFGNSAVVLQNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPYENL 486
            GT+DFIFG++AVV Q C I P+ PL  Q NTITAQG+ DPNQ++G+S+Q  TI    N+
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNV 489

Query: 487 KTTETYLGRPWKNYSTTVFVQSNLGSLIHPTGWLPWV-GTSAPDTIFYAEFQNNGPGSSK 546
               TYLGRPWK +STTV +++ +G+++ P+GW+ WV G   P +I Y E++N GPGS  
Sbjct: 490 -IAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 VKRVKWKGVKNI-DIKTAKKFTVSSFIHGKDWI 555
            +RVKW G K +     A KFTV++ +HG DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

BLAST of Sed0000177 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 392.9 bits (1008), Expect = 4.4e-109
Identity = 227/556 (40.83%), Postives = 321/556 (57.73%), Query Frame = 0

Query: 27  IAVIVLSSVVLVGVVLGVVFGTRNGSKSDDRNNQNGALSTSIKAVCDVTLYPDTCYNSFA 86
           IA ++ + +VL+ V +G+               Q    +T+++AVC  T Y +TC NS  
Sbjct: 14  IAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSLM 73

Query: 87  PFANSSHLDPGQISKLSVQLALGELSQVANYIFEHAITNSTDDK-TILALSSCHELLDLA 146
             A+     P  + KL   + +  +              + +DK T  AL  C +L++ A
Sbjct: 74  K-ASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMNDA 133

Query: 147 IDHLNISLSSSD----ITLLKAVDDLKTWLTSAATYQQTCIDDLEDVDPSLANMVASYLK 206
            D L   L + D      +   V+DL+ WL+ +  YQQTC+D  E+ +  L+  +    K
Sbjct: 134 TDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFK 193

Query: 207 KSTELTSNGLAIVTFFSNLTDSFSL-----------RRLMSYEDHESNGDWARPILRKLA 266
            S ELTSNGLA++T  SNL   F++           R+L+S ED      W  P  R+L 
Sbjct: 194 TSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAED--GIPSWVGPNTRRLM 253

Query: 267 QTDLRKQVNIVVAKGGSGKYKTIGAALKAVPDKSKKRTVIYVKKGIYNENVEVGKNKWNV 326
            T    + N+VVA  GSG+YKTI  AL AVP  ++K  VIY+K+G+YNE V+V K   +V
Sbjct: 254 ATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKMTHV 313

Query: 327 VMIGDGMTSTTVTGRLNFIDG-TPTFQSATFAAKGKGFIAIDMGFKNTAGPSKHQAVALM 386
             IGDG T T +TG LN+  G   T+ +AT A  G  F A ++GF+NTAGP  HQAVAL 
Sbjct: 314 TFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAVALR 373

Query: 387 STSDQSIFYRCQMDAYQDTLYAHSNRQFYRDCKVFGTVDFIFGNSAVVLQNCIIAPKLPL 446
            ++D ++FY CQ+D YQDTLY HS+RQF+RDC V GTVDFIFG+  VVLQNC I  + P+
Sbjct: 374 VSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVRKPM 433

Query: 447 LGQKNTITAQGRFDPNQNTGISLQGSTI---QPYENLKT-TETYLGRPWKNYSTTVFVQS 506
             Q   ITAQGR D  ++TG+ LQ   I     Y  +K+  + YLGRPWK +S T+ + +
Sbjct: 434 KSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTIIMGT 493

Query: 507 NLGSLIHPTGWLPWVGTSAPDTIFYAEFQNNGPGSSKVKRVKWKGVKNIDIKTAKKFTVS 562
            +  +I P GWLPW G  A +T++YAE++NNGPGS++ +RVKW G+K +  K A +FT +
Sbjct: 494 TIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPA 553

BLAST of Sed0000177 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.8e-107
Identity = 229/521 (43.95%), Postives = 307/521 (58.93%), Query Frame = 0

Query: 57  RNNQNGALSTSIKAVCDVTLYPDTCYNSFAPF-ANSSHLDPGQISKLSVQLALGELSQVA 116
           R + +   S+ ++ VC+VT YP +C +S +    + +  DP  + +LS+Q+   EL+ + 
Sbjct: 448 RKSPHPTPSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIV 507

Query: 117 NYIFEHAITNSTDDKTI-LALSSCHELLDLAIDHLNISLSSSDITL--------LKAVDD 176
                  +   T+D+ +  ALS C ++ DLA+D +N ++SS D  +           + D
Sbjct: 508 G--LPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGD 567

Query: 177 LKTWLTSAATYQQTCIDDLED--VDPSLANMVASYLKKSTELTSNGLAIVTFFSNLTDSF 236
           L TWL+SA T   TC D L++   +  +   + S +  STE TSN LAIV          
Sbjct: 568 LITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKS 627

Query: 237 SL----RRLMSYEDHESNGDWARPILRKLAQTDLRKQVNIVVAKGGSGKYKTIGAALKAV 296
            +    RRL+   +  S  +W RP +R+L Q       ++ VA  GSG  +T+  A+  V
Sbjct: 628 RIPVQGRRLL---NSNSFPNWVRPGVRRLLQAK-NLTPHVTVAADGSGDVRTVNEAVWRV 687

Query: 297 PDKSKKRTVIYVKKGIYNENVEVGKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFQSATF 356
           P K K   VIYVK G Y ENV + K+KWNV + GDG   T ++G  N +DG  TF ++TF
Sbjct: 688 PKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTF 747

Query: 357 AAKGKGFIAIDMGFKNTAGPSKHQAVALMSTSDQSIFYRCQMDAYQDTLYAHSNRQFYRD 416
           A +GKGF+  DMG  NTAGP KHQAVA  S SD+S++YRC  D YQDTLY HSNRQ+YR+
Sbjct: 748 ATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRN 807

Query: 417 CKVFGTVDFIFGNSAVVLQNCIIAPKLPLLGQKNTITAQGRFDPNQNTGISLQGSTIQPY 476
           C V GTVDFIFG   VV Q C I P+ PL  Q NTITA+G  + NQNTGIS+   TI P 
Sbjct: 808 CDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPN 867

Query: 477 ENLKTTETYLGRPWKNYSTTVFVQSNLGSLIHPTGWLPWVGT--SAPDTIFYAEFQNNGP 536
            N+ T  TYLGRPWK +S TV +QS +GS ++P GW+ W  T    P TIFY E++N+GP
Sbjct: 868 GNV-TATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGP 927

Query: 537 GSSKVKRVKWKGVKNIDI-KTAKKFTVSSFIHGKD-WISKA 558
           GS   KRVKW G K I     A +FTV  F+ G D WI KA
Sbjct: 928 GSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895610.14.2e-27986.24putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida][more]
XP_022927225.19.6e-27685.16putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata][more]
KAG6583848.12.8e-27584.98putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosp... [more]
XP_004135547.18.2e-27584.48putative pectinesterase/pectinesterase inhibitor 24 [Cucumis sativus] >KGN65919.... [more]
XP_023520176.11.2e-27384.45putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SG773.8e-16653.95Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3... [more]
Q9FF785.3e-16052.29Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3... [more]
Q431114.5e-13546.39Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1[more]
Q438671.5e-12243.63Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1[more]
Q9FJ216.2e-10840.83Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1ENB04.7e-27685.16Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1[more]
A0A0A0LY084.0e-27584.48Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G538170 PE=3 SV=1[more]
A0A6J1KL237.5e-27484.45Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1[more]
A0A5A7VDG17.5e-27484.48Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G0077... [more]
A0A1S3BDA47.5e-27484.48Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10710.12.7e-16753.95root hair specific 12 [more]
AT5G04960.13.8e-16152.29Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G53840.11.1e-12343.63pectin methylesterase 1 [more]
AT5G49180.14.4e-10940.83Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G14300.11.8e-10743.95pectinesterase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Chayote (edule) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 63..213
e-value: 7.4E-31
score: 118.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 67..213
e-value: 1.6E-25
score: 90.2
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 52..214
e-value: 1.7E-23
score: 80.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 254..565
e-value: 1.2E-109
score: 368.6
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 259..550
e-value: 1.1E-136
score: 454.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 65..217
e-value: 7.9E-31
score: 109.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 67..218
NoneNo IPR availablePANTHERPTHR31707:SF272PECTINESTERASE/PECTINESTERASE INHIBITOR 24-RELATEDcoord: 18..561
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 18..561
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 70..215
e-value: 9.51066E-38
score: 134.873
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 403..412
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 284..303
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 258..562

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sed0000177.1Sed0000177.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity