Lsi10G009660 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi10G009660 vs. ExPASy Swiss-Prot
Match: M4IRL9 (Probable CoA ligase CCL12 OS=Humulus lupulus OX=3486 GN=CCL12 PE=2 SV=1)

HSP 1 Score: 1063.9 bits (2750), Expect = 8.1e-310
Identity = 511/734 (69.62%), Postives = 619/734 (84.33%), Query Frame = 0

Query: 7   ELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHPYGLHRL 66
           E+G+EDLVKAGL+ +EA  F R+L++ V+ AK  DP+EVWRE++AR+ LKP+HP+ LH+L
Sbjct: 3   EVGVEDLVKAGLSTEEAKYFQRVLEDIVNGAKGLDPSEVWREVVARRLLKPSHPHELHQL 62

Query: 67  LYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISSFSLFQK 126
           LY+SVYANW++S+ GPPLYWFPSL QSK TNLG++ME +G ++LG  YKDPI+SFSLF+K
Sbjct: 63  LYHSVYANWDVSTRGPPLYWFPSLHQSKKTNLGRLMEKYGSEVLGPLYKDPITSFSLFRK 122

Query: 127 FSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKS--NGRWLPDSILNIAECCLLPSSRP 186
           FS Q PE YWSIVL+ELSV+FQ+AP+CILD  +KS  +G  LP S+LNIAECCLLP+S P
Sbjct: 123 FSVQQPEAYWSIVLKELSVSFQEAPKCILDSADKSKRSGSLLPGSVLNIAECCLLPTSYP 182

Query: 187 LKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPLTVDA 246
           +K D+  AIVWRDEG DD+ +N +TLKELREKVMLVANALDA FSKGDAIAIDMP+TV+A
Sbjct: 183 MKRDDSPAIVWRDEGYDDSEVNVLTLKELREKVMLVANALDAMFSKGDAIAIDMPMTVNA 242

Query: 247 VVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSRVIER 306
           VVIYLAI+L+G VVVSIADSFAA E+A RLR+S AK IFTQDFILRGG+K+ LYSRV++ 
Sbjct: 243 VVIYLAIILSGFVVVSIADSFAAKEIATRLRVSNAKAIFTQDFILRGGRKFSLYSRVVDA 302

Query: 307 GSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNILFSSG 366
              KAIV+P  G+ L + LREQD+SW++FIS      RSN Y+P Y  +D++ NILFSSG
Sbjct: 303 APHKAIVLPVNGSNLGLQLREQDISWEKFISNVSNQARSNHYTPFYQPVDSLINILFSSG 362

Query: 367 TTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAGATLA 426
           TTGEPKAIPWTQLSP+R AAD+WAH+D Q GDV CWPTNLGWVMGPV ++S FL+GATLA
Sbjct: 363 TTGEPKAIPWTQLSPLRCAADTWAHIDAQVGDVFCWPTNLGWVMGPVLLFSSFLSGATLA 422

Query: 427 LYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLDWTKI 486
           LYHGSPLG GFGKFVQ             D+ VT+LGTVPSLVK WK+T+C++GLDWTKI
Sbjct: 423 LYHGSPLGHGFGKFVQ-------------DSGVTILGTVPSLVKAWKNTQCMKGLDWTKI 482

Query: 487 RHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFSTASMT 546
           + FASTGE SNVDDDLWLSS++YY+PI+E CGGTELASSYI GS +QPQAFGAFSTA+MT
Sbjct: 483 KVFASTGEASNVDDDLWLSSQAYYQPIIECCGGTELASSYIQGSLVQPQAFGAFSTAAMT 542

Query: 547 TGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQLRRH 606
            G VILDE+GIPYP+   C GE+GLFPLYLGA++RLLNA++++VYFKGMP+YNGM LRRH
Sbjct: 543 VGLVILDENGIPYPDKQECTGEVGLFPLYLGATDRLLNADNEKVYFKGMPLYNGMSLRRH 602

Query: 607 GDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPIEGGP 666
           GDI+KR+ GG+F+V GRADDTMNLGGIKTSS+EIER+C+  DES++ET AVSV+P+ GGP
Sbjct: 603 GDILKRTAGGFFIVHGRADDTMNLGGIKTSSIEIERVCNRADESVMETTAVSVAPVSGGP 662

Query: 667 EQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVL 726
           EQL++ VVL+ G+    + LK KFSKAIQSNLNPLFKV FVKIV  FPRTASNKLLRRVL
Sbjct: 663 EQLVMFVVLKSGHNNEAERLKKKFSKAIQSNLNPLFKVSFVKIVEEFPRTASNKLLRRVL 722

Query: 727 RKQMKDELSLQSQL 739
           R QMK EL ++S++
Sbjct: 723 RDQMKHELLVRSKI 723

BLAST of Lsi10G009660 vs. ExPASy Swiss-Prot
Match: Q84P17 (Probable acyl-activating enzyme 18, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE18 PE=2 SV=1)

HSP 1 Score: 1030.0 bits (2662), Expect = 1.3e-299
Identity = 493/741 (66.53%), Postives = 605/741 (81.65%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           M K+I EL  +D VKAGL L++A +F++++ + ++ A  +DP + W+ L+    LKP HP
Sbjct: 1   MWKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           + LH+LLYYSVY+NW+ S +GPPLYWFPSL QSK TNLGK+ME HGP+LLG SYK+P+ S
Sbjct: 61  HPLHQLLYYSVYSNWDSSVHGPPLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLES 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           F LF++FS ++PE+YWS V++ELS+ F   PRCIL+++ K  G WLPD++LNIAECCL+P
Sbjct: 121 FELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNKS-KPEGTWLPDAVLNIAECCLMP 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SS P K+D+ +A+VWR+EG DD+ +N MT+KELRE+VMLVANA+  +F KGD IAIDMP+
Sbjct: 181 SSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISGSFEKGDTIAIDMPM 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAV+IYLAI+LAG +VVSIADSFAA E+A RL+ISKAKGIFTQD+ILRGG+++PLYSR
Sbjct: 241 TVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGGRRFPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPR-SNCYSPIYHSIDAVTNI 360
           V+E    K IV+PA+G  L V LREQD+SW +F+S AK      N Y PIY  +++V NI
Sbjct: 301 VVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYLPVESVINI 360

Query: 361 LFSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLA 420
           LFSSGTTGEPKAIPWTQLSPIRSA D WAHLDVQ G   CWPTNLGWVMGP  ++SCFL 
Sbjct: 361 LFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPTLMFSCFLT 420

Query: 421 GATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGL 480
           GATLALY GSPLGRGFGKFVQ             DA VT+LGTVPSLVKTWK T C+EGL
Sbjct: 421 GATLALYSGSPLGRGFGKFVQ-------------DAGVTVLGTVPSLVKTWKRTNCMEGL 480

Query: 481 DWTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFS 540
           +WTKI+ FA+TGE SNVDD LWLSS++ YKP++E CGGTELASSYI GSPLQPQAFGAFS
Sbjct: 481 NWTKIKFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFS 540

Query: 541 TASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGM 600
           T SMTT  +I DE+G+PYP+D PC GE+GLFP +LGA++RLLNANHDEVYFKGMP+Y   
Sbjct: 541 TPSMTTRIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKET 600

Query: 601 QLRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSP 660
           +LRRHGDI+KR+VGGY+ VQGRADDTMNLGGIKTSS+EIER+CD  DE I ETAAV+++P
Sbjct: 601 RLRRHGDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTP 660

Query: 661 IEGGPEQLIILVVLRKGYK-RFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNK 720
             GGPE L+I  VL++G+K +  +ELK+KFS+ IQ +LNPLFKV FVKIVP FPRTAS+K
Sbjct: 661 PNGGPELLVIFAVLKEGFKQQSGEELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSK 720

Query: 721 LLRRVLRKQMKDE-LSLQSQL 739
           LLRRVLR Q+K E LSL+S++
Sbjct: 721 LLRRVLRDQIKQELLSLRSRI 727

BLAST of Lsi10G009660 vs. ExPASy Swiss-Prot
Match: F4KBF3 (Probable acyl-activating enzyme 17, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE17 PE=2 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 6.6e-203
Identity = 370/743 (49.80%), Postives = 494/743 (66.49%), Query Frame = 0

Query: 3   KNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHPYG 62
           K++  +   D+   G++ D + K  R L++ +  +  S P E W ++ +R+ L P  P+ 
Sbjct: 4   KSLNSITKSDIEALGISGDVSEKLLRDLEDIIHGS--STPPETWIQI-SRRILHPNLPFS 63

Query: 63  LHRLLYYSVYANWNISSNGPPL-YWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISSF 122
            H+++YY  Y ++     GP L  W P    + LTN+GK++E  G + LG +YK+P+SSF
Sbjct: 64  FHQMMYYGCYKDF-----GPDLPAWIPDPKVASLTNVGKLLEKRGKEFLGGNYKNPVSSF 123

Query: 123 SLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRT---EKSNGRWLPDSILNIAECCL 182
           S FQ+FS  NPE+YW  VL+EL++ F   P+CIL++    +   G+WLP + LN A  CL
Sbjct: 124 SSFQEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGDNPGGKWLPGAYLNPARNCL 183

Query: 183 LPSSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDAT-FSKGDAIAID 242
               +   DD  + I WRDEG DD  +N MTL ELR +V L A+AL A    +  AIA+D
Sbjct: 184 TNGFKRRLDD--IVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEEESAIAVD 243

Query: 243 MPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPL 302
           MP+ V++V+IYLAIVLAG VVVSIADSF+  E++ RL+ISKAK IFTQD I+RG K  PL
Sbjct: 244 MPMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIRGDKSIPL 303

Query: 303 YSRVIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVT 362
           Y RV++  +  AIV+PA G+   + LRE+DLSW  F+  A+   R   Y        A T
Sbjct: 304 YRRVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGNARNL-RGVEYVAAEKPAGAYT 363

Query: 363 NILFSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCF 422
           NILFSSGTTGEPKAIPWT +SP++SAAD+W HLDVQ GDV+ WPTNLGW+MGP  VY+  
Sbjct: 364 NILFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGDVVAWPTNLGWMMGPWLVYASL 423

Query: 423 LAGATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLE 482
           + GA + LY+GSPLG  F KFVQ             DAEV++LG +PS+V+TW+++    
Sbjct: 424 INGACMGLYNGSPLGPTFAKFVQ-------------DAEVSVLGVIPSIVRTWQNSNSTS 483

Query: 483 GLDWTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGA 542
           G DW++IR F STGE SN+D+ LWL  R++YKP++E+CGGTE+  S+I+GS LQPQ+  A
Sbjct: 484 GYDWSRIRCFGSTGEASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAA 543

Query: 543 FSTASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYN 602
           FSTA+M     ILDE   P P     +GE+ L P   GAS+ LLN NH +VYF+GMP + 
Sbjct: 544 FSTAAMGCKLFILDEDSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYFQGMPTFQ 603

Query: 603 GMQLRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSV 662
           G  LRRHGD+ +R+  GY+   GRADDTMNLGGIK  S+EIER+C+ VD+S+LETAA+ V
Sbjct: 604 GQILRRHGDLFERTSKGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGV 663

Query: 663 SPIEGGPEQLIILVVLRKGYKRFP--QELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTA 722
            P  GGPEQL+I VV +      P    LK  F+  IQ  LNPLFKV  V  +PS PRTA
Sbjct: 664 PPPSGGPEQLVIAVVFKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTA 721

Query: 723 SNKLLRRVLRKQMKDELSLQSQL 739
           +NK++RRVLR+Q+  +  L S+L
Sbjct: 724 TNKVMRRVLRQQL-TQTGLNSKL 721

BLAST of Lsi10G009660 vs. ExPASy Swiss-Prot
Match: Q88EH6 (Acetyl-coenzyme A synthetase 1 OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) OX=160488 GN=acsA1 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 1.2e-39
Identity = 178/650 (27.38%), Postives = 295/650 (45.38%), Query Frame = 0

Query: 107 PKLLGASYKDPISSFSLFQKFSFQNPEIYWSIVLEELS--VTFQKAPRCILDRTEKSNGR 166
           P++   +  D  +  +++Q+ S  NP+ +W    + +     F K  +   D     + +
Sbjct: 11  PEVAATTLTDEATYKAMYQQ-SVINPDGFWREQAQRIDWIKPFTKVKQTSFD-DHHVDIK 70

Query: 167 WLPDSILNIAECCLLPSSRPLKD-DNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANA 226
           W  D  LN++  CL    R L++  + LAI+W  EG D +   ++T +EL E+V   ANA
Sbjct: 71  WFADGTLNVSSNCL---DRHLEERGDQLAIIW--EGDDPSEHRNITYRELHEQVCKFANA 130

Query: 227 L-DATFSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGI 286
           L      +GD + I MP+  +AVV  LA    G +   +   F+   +A R+   K+K +
Sbjct: 131 LRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCKSKVV 190

Query: 287 FTQDFILRGGKKYPLYSRV------IERGSC-KAIVIPATGNRLEVDLREQDLSWQEFIS 346
            T D  +RGG++ PL + V       E  S  K IV   TG  +    + +D+ +++ + 
Sbjct: 191 ITADEGVRGGRRTPLKANVDLALTNPETSSVQKIIVCKRTGGDIAWH-QHRDIWYEDLMK 250

Query: 347 TAKEFPRSNCYSPIYHSIDAVTNILFSSGTTGEPKAIPWTQLSPIRSAADSWAHL-DVQA 406
            A     S+C +P     +    IL++SG+TG+PK +  T    +  AA +   + D + 
Sbjct: 251 VAS----SHC-APKEMGAEEALFILYTSGSTGKPKGVLHTTGGYLVYAALTHERVFDYRP 310

Query: 407 GDVLCWPTNLGWVMG-PVSVYSCFLAGATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMH 466
           G+V     ++GWV G    VY     GAT  L+ G P      +          ++V+ H
Sbjct: 311 GEVYWCTADVGWVTGHSYIVYGPLANGATTLLFEGVPNYPDITRV--------SKIVDKH 370

Query: 467 DAEVTLLGTVPSLVKTW--KDTRCLEGLDWTKIRHFASTGETSNVDDDLWLSSRSYYK-- 526
             +V +L T P+ ++    +    +EG D + +R   S GE  N +   W     YYK  
Sbjct: 371 --KVNILYTAPTAIRAMMAEGQAAVEGADGSSLRLLGSVGEPINPEAWNW-----YYKTV 430

Query: 527 -----PILEFCGGTELASSYITGSPLQPQAFGAFSTASMTTGF-----VILDEHGIPYPE 586
                PI++    TE     I  SPL P A G     S T  F      ++D  G     
Sbjct: 431 GKERCPIVDTWWQTETGGILI--SPL-PGATG-LKPGSATRPFFGVVPALVDNLGNLI-- 490

Query: 587 DHPCIGEIGLFPLYLGASNRLLNANHD---EVYFKGMPVYNGMQLRRHGDIIKRSVGGYF 646
           D    G + +   + G S R L  +HD   + YFK    + GM     GD  +R   GY+
Sbjct: 491 DGAAEGNLVILDSWPGQS-RSLYGDHDRFVDTYFK---TFRGMYFT--GDGARRDEDGYY 550

Query: 647 VVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPIEGGPEQLIILVVLRKG 706
            + GR DD +N+ G +  + EIE     V  S +  AAV   P +   + + + V L  G
Sbjct: 551 WITGRVDDVLNVSGHRMGTAEIESAM--VAHSKVAEAAVVGVPHDIKGQGIYVYVTLNAG 610

Query: 707 YKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRK 727
            +   ++L+++    ++  + P+     ++  P  P+T S K++RR+LRK
Sbjct: 611 IEA-SEQLRLELKNWVRKEIGPIASPDVIQWAPGLPKTRSGKIMRRILRK 617

BLAST of Lsi10G009660 vs. ExPASy Swiss-Prot
Match: Q4ZQG8 (Acetyl-coenzyme A synthetase OS=Pseudomonas syringae pv. syringae (strain B728a) OX=205918 GN=acsA PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 3.6e-39
Identity = 174/656 (26.52%), Postives = 287/656 (43.75%), Query Frame = 0

Query: 107 PKLLGASYKDPISSFSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGR-- 166
           P++   +  D  +  +++Q+ S  NP+ +W    + L       P  ++ +T   + R  
Sbjct: 11  PEVAATTLTDEATYKAMYQQ-SVVNPDGFWREQAQRLDWI---KPFSVVKQTSFDDHRVD 70

Query: 167 --WLPDSILNIAECCLLPSSRPLKD-DNHLAIVWRDEGCDDANMNHMTLKELREKVMLVA 226
             W  D  LN+A  CL    R L++  + +AI+W  EG D +    +T +EL  +V   A
Sbjct: 71  IKWFADGTLNVAYNCL---DRHLEERGDSIAIIW--EGDDPSEHREITYRELHAEVCKFA 130

Query: 227 NAL-DATFSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAK 286
           NAL      +GD + I MP+  +AVV  LA    G +   +   F+   +A R+    +K
Sbjct: 131 NALRGQDVHRGDVVTIYMPMIPEAVVAMLACARIGAIHSVVFGGFSPEALAGRIIDCSSK 190

Query: 287 GIFTQDFILRGGKKYPLYSRV------IERGSC-KAIVIPATGNRLEVDLREQDLSWQEF 346
            + T D  LRGGKK  L + V       E  S  K IV   TG  +E + R +D+ +   
Sbjct: 191 VVITADEGLRGGKKTALKANVDRALTNPETSSVQKVIVCKRTGGEIEWN-RHRDIWYHSL 250

Query: 347 ISTAKEFPRSNCYSPIYHSIDAVTNILFSSGTTGEPKAIPWTQLSPIRSAADSWAHL-DV 406
           +  A     S+  +P     +    IL++SG+TG+PK +  T    +  AA +   + D 
Sbjct: 251 LEVA-----SSTCAPKEMGAEESLFILYTSGSTGKPKGVLHTTAGYLLYAALTHERVFDY 310

Query: 407 QAGDVLCWPTNLGWVMG-PVSVYSCFLAGATLALYHGSPLGRGFGKFVQGQMGCPMRLVE 466
           + G+V     ++GWV G    VY     GAT  L+ G P      +          ++++
Sbjct: 311 RPGEVYWCTADVGWVTGHSYIVYGPLANGATTLLFEGVPNYPDITRV--------SKIID 370

Query: 467 MHDAEVTLLGTVPSLVKTW--KDTRCLEGLDWTKIRHFASTGETSNVDDDLWLSSRSYYK 526
            H  +V +L T P+ ++    + T+ +EG D + +R   S GE  N +   W     YY 
Sbjct: 371 KH--KVNILYTAPTAIRAMMAEGTKSVEGADGSSLRLLGSVGEPINPEAWAW-----YYN 430

Query: 527 -------PILEFCGGTELASSYITGSPLQPQAFGAFSTASMTTGF-----VILDEHGIPY 586
                  PI++    TE     I+  P       A    S T  F      ++D  G   
Sbjct: 431 TVGKQNCPIVDTWWQTETGGILISPLP----GATALKPGSATRPFFGVIPALVDNLGNLI 490

Query: 587 PEDHPCIGEIGLFPLYLGASNRLLNANHD---EVYFKGMPVYNGMQLRRHGDIIKRSVGG 646
             +    G + +   + G S R L  +HD   + YFK    + GM     GD  +R   G
Sbjct: 491 --EGAAEGNLVILDSWPGQS-RSLYGDHDRFVDTYFK---TFRGMYFT--GDGARRDEDG 550

Query: 647 YFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPIEGGPEQL----IIL 706
           YF + GR DD +N+ G +  + EIE        +++    V+ + + G P  L    I +
Sbjct: 551 YFWITGRVDDVLNVSGHRMGTAEIE-------SAMVAHPKVAEAAVVGVPHDLKGQGIYV 610

Query: 707 VVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRK 727
            V   G +     L+ +    ++  + P+    F++  P  P+T S K++RR+LRK
Sbjct: 611 YVTLNGGEEPSDALRTELRNWVRKEIGPIASPDFIQWAPGLPKTRSGKIMRRILRK 617

BLAST of Lsi10G009660 vs. ExPASy TrEMBL
Match: A0A0A0L1Q9 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280430 PE=4 SV=1)

HSP 1 Score: 1328.5 bits (3437), Expect = 0.0e+00
Identity = 654/738 (88.62%), Postives = 690/738 (93.50%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           MGK++WEL IEDLVK GLNL EA++FNRILQN +STA+RS PTEVWRELMAR+ALKPTHP
Sbjct: 1   MGKSVWELDIEDLVKTGLNLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           Y LH LLYYSVYANWNISSNGPP YWFPS++QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           FSLFQK S+Q+PEIYWSIVL ELSVTFQK PRCILDRTEKSN RWLPDSILNIAECCLLP
Sbjct: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SSRPLKDDN  AIVWR+EGCD++N+NHMTLK+LREKV LVANALDATFSKGDAIAIDMPL
Sbjct: 181 SSRPLKDDNSSAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNIL 360
           VI+ GSCKAIVIPATGNRL+VDLREQDLSW+EF+STAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAG 420
           FSSGTTGEPKAIPWTQ SPIR AADSWAH+DVQ GDV CWPTNLGWVMGPVSVYS  LAG
Sbjct: 361 FSSGTTGEPKAIPWTQRSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420

Query: 421 ATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLD 480
           ATLALYHGSPLG GFGKFVQ             DA VTLLGTVPSLVKTWKDTRC+EGL+
Sbjct: 421 ATLALYHGSPLGYGFGKFVQ-------------DAGVTLLGTVPSLVKTWKDTRCMEGLN 480

Query: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFST 540
           WTKIRHFASTGETSNVDDDLWLSSRSYYKPI EFCGGTELASSYI G+PLQPQAFGAF+T
Sbjct: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTT 540

Query: 541 ASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQ 600
           ASMTT FVILDEHGIPYPED PCIGEIGLFP+YLGASN LLNA+HD+VYFKGMP+YNGMQ
Sbjct: 541 ASMTTEFVILDEHGIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQ 600

Query: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPI 660
           LRRHGDIIKR+VGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVS+SPI
Sbjct: 601 LRRHGDIIKRTVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPI 660

Query: 661 EGGPEQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720
           EGGPEQL+ILVVLRK Y+R PQELKVKFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLL
Sbjct: 661 EGGPEQLVILVVLRKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLL 720

Query: 721 RRVLRKQMKDELSLQSQL 739
           RRVLRKQMKDEL+L+SQL
Sbjct: 721 RRVLRKQMKDELALRSQL 725

BLAST of Lsi10G009660 vs. ExPASy TrEMBL
Match: A0A5D3DDN3 (Putative acyl-activating enzyme 18, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00570 PE=3 SV=1)

HSP 1 Score: 1319.7 bits (3414), Expect = 0.0e+00
Identity = 646/738 (87.53%), Postives = 689/738 (93.36%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           MGK+IWELGIEDLVK GLNL EA++FNRIL+N +STA+RS P EVWRELMA++ALKPTHP
Sbjct: 1   MGKSIWELGIEDLVKTGLNLHEAVEFNRILKNVLSTAQRSKPAEVWRELMAKRALKPTHP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           + LHRLLYYSVYANWNIS+NGPP YWFPSL+QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTNGPPPYWFPSLNQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           FSLFQKFS QNPEIYWSIVL+ELSVTFQKAPRCILDRTEK NGRWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDN LAIVWRDEGCD++N+NHMTLK+LR+KV LVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLRDKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNIL 360
           VIE GSCKAIVIP TGNRLEVDLREQDLSW+EF+STAKEFPRSNCYSP+YHSIDAVTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAG 420
           FSSGTTGEPKAIPWTQLSPIR AADSWAH+DVQAGDV CWPTNLGW MGPVSVYS  L G
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLD 480
           ATLALYHGSPLG GFGKFVQ             DA VT+LGTVPSLVKTWKDTRC+EGL+
Sbjct: 421 ATLALYHGSPLGHGFGKFVQ-------------DAGVTILGTVPSLVKTWKDTRCMEGLN 480

Query: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFST 540
           WTKIR+FASTGETSNVDDDLWLSSRSYYKP+ E CGGTEL SS+I GSPLQPQAFGAFST
Sbjct: 481 WTKIRYFASTGETSNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPLQPQAFGAFST 540

Query: 541 ASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQ 600
           ASMTTGFVILDEHGIP+PED PCIGEIGLFP+YLGASN LLNA+HDEVYFKGMP+YNGMQ
Sbjct: 541 ASMTTGFVILDEHGIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQ 600

Query: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPI 660
           LRRHGDIIKR+VGGY VVQGRADDTMNLGGIKTSSVE+ERICD VDESILETAAV+VSPI
Sbjct: 601 LRRHGDIIKRTVGGYLVVQGRADDTMNLGGIKTSSVELERICDLVDESILETAAVAVSPI 660

Query: 661 EGGPEQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720
           +GGPEQL+ILVVL +GY++ PQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL
Sbjct: 661 QGGPEQLVILVVLSEGYRKSPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720

Query: 721 RRVLRKQMKDELSLQSQL 739
           RRVLRKQMKDEL+LQS L
Sbjct: 721 RRVLRKQMKDELALQSHL 725

BLAST of Lsi10G009660 vs. ExPASy TrEMBL
Match: A0A1S3BNK5 (probable acyl-activating enzyme 18, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103491592 PE=3 SV=1)

HSP 1 Score: 1319.7 bits (3414), Expect = 0.0e+00
Identity = 646/738 (87.53%), Postives = 689/738 (93.36%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           MGK+IWELGIEDLVK GLNL EA++FNRIL+N +STA+RS P EVWRELMA++ALKPTHP
Sbjct: 1   MGKSIWELGIEDLVKTGLNLHEAVEFNRILKNVLSTAQRSKPAEVWRELMAKRALKPTHP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           + LHRLLYYSVYANWNIS+NGPP YWFPSL+QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTNGPPPYWFPSLNQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           FSLFQKFS QNPEIYWSIVL+ELSVTFQKAPRCILDRTEK NGRWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDN LAIVWRDEGCD++N+NHMTLK+LR+KV LVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLRDKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNIL 360
           VIE GSCKAIVIP TGNRLEVDLREQDLSW+EF+STAKEFPRSNCYSP+YHSIDAVTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAG 420
           FSSGTTGEPKAIPWTQLSPIR AADSWAH+DVQAGDV CWPTNLGW MGPVSVYS  L G
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLD 480
           ATLALYHGSPLG GFGKFVQ             DA VT+LGTVPSLVKTWKDTRC+EGL+
Sbjct: 421 ATLALYHGSPLGHGFGKFVQ-------------DAGVTILGTVPSLVKTWKDTRCMEGLN 480

Query: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFST 540
           WTKIR+FASTGETSNVDDDLWLSSRSYYKP+ E CGGTEL SS+I GSPLQPQAFGAFST
Sbjct: 481 WTKIRYFASTGETSNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPLQPQAFGAFST 540

Query: 541 ASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQ 600
           ASMTTGFVILDEHGIP+PED PCIGEIGLFP+YLGASN LLNA+HDEVYFKGMP+YNGMQ
Sbjct: 541 ASMTTGFVILDEHGIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQ 600

Query: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPI 660
           LRRHGDIIKR+VGGY VVQGRADDTMNLGGIKTSSVE+ERICD VDESILETAAV+VSPI
Sbjct: 601 LRRHGDIIKRTVGGYLVVQGRADDTMNLGGIKTSSVELERICDLVDESILETAAVAVSPI 660

Query: 661 EGGPEQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720
           +GGPEQL+ILVVL +GY++ PQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL
Sbjct: 661 QGGPEQLVILVVLSEGYRKSPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720

Query: 721 RRVLRKQMKDELSLQSQL 739
           RRVLRKQMKDEL+LQS L
Sbjct: 721 RRVLRKQMKDELALQSHL 725

BLAST of Lsi10G009660 vs. ExPASy TrEMBL
Match: A0A5A7TAS8 (Putative acyl-activating enzyme 18, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00370 PE=3 SV=1)

HSP 1 Score: 1310.4 bits (3390), Expect = 0.0e+00
Identity = 641/738 (86.86%), Postives = 686/738 (92.95%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           MGK+IWELGIEDLV+ GLNL EA++FNRIL+N +ST +RS PTEVWRELMAR+ALKPTHP
Sbjct: 1   MGKSIWELGIEDLVRTGLNLHEAVEFNRILKNVLSTTQRSKPTEVWRELMARRALKPTHP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           + LHRLLYYSVYANWNIS++GPP YWFPSLDQSKLTNLGKIMEL GPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTSGPPPYWFPSLDQSKLTNLGKIMELQGPKLLGASYKDPISS 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           FSLFQKFS QNPEIYWSIVL+ELSVTFQKAPRCILDRTEK NGRWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDN LAIVWRDEGCD++N+NHMTLK+LREKV LVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNIL 360
           VIE GSCKAIVIP TGNRLEVDLREQDLSW+EF+STAKEFPRSNCYSP+YHSIDAVTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAG 420
           FSSGTTG+PKAIPWTQLSPIR AADSWAH+DVQAGDV CWPTNLGW MGPVSVYS  L G
Sbjct: 361 FSSGTTGKPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLD 480
           ATLALYHGSPLG GFGKFVQ             DA VT+LGT+PSLVKTWKDTRC+EGL+
Sbjct: 421 ATLALYHGSPLGHGFGKFVQ-------------DAGVTILGTIPSLVKTWKDTRCMEGLN 480

Query: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFST 540
           WTKIR+FASTGETSNVDDDLWLSSRSYYKP+ E CGGTEL SS+I GSP+QPQAFGAFST
Sbjct: 481 WTKIRYFASTGETSNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFST 540

Query: 541 ASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQ 600
           ASMTTGFVILDEHGIP+PED PCIGEIGLFP+YLGASN LLNA+HDEVYFKGMP+YNGMQ
Sbjct: 541 ASMTTGFVILDEHGIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQ 600

Query: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPI 660
           LRRHGDII+R+VGGY +VQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVS+SPI
Sbjct: 601 LRRHGDIIRRTVGGYLIVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPI 660

Query: 661 EGGPEQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720
           EGGPEQL+ILVVL+K Y +  QELK KFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLL
Sbjct: 661 EGGPEQLVILVVLKKQYVKSSQELKDKFSKAIQRNLNPLFKVGFVKIVPSFPRTASNKLL 720

Query: 721 RRVLRKQMKDELSLQSQL 739
           RRVLRKQMKDEL+L+SQL
Sbjct: 721 RRVLRKQMKDELALRSQL 725

BLAST of Lsi10G009660 vs. ExPASy TrEMBL
Match: A0A6J1DJZ2 (probable acyl-activating enzyme 18, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111021232 PE=4 SV=1)

HSP 1 Score: 1300.4 bits (3364), Expect = 0.0e+00
Identity = 631/738 (85.50%), Postives = 681/738 (92.28%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           MGKNIWELG+ED VKAGLNL+EA++F+RILQN V   KRSDP EVWRELMAR+ LKPTHP
Sbjct: 1   MGKNIWELGVEDTVKAGLNLEEAVEFDRILQNVVLVPKRSDPREVWRELMARRVLKPTHP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           YGLH+L+YYSVYANW+ SSNGPP YWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YGLHQLVYYSVYANWDSSSNGPPPYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           +SLFQKFS Q+PEIYWSIVLEELSVTF K PRCILD  EKS GRWLPDSILNIAECCLLP
Sbjct: 121 YSLFQKFSSQHPEIYWSIVLEELSVTFHKEPRCILDMAEKSKGRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SSRPL+DDN LAIVWRDEG D++N+N MTLKELREKV LVANALDA FSKGDAIAIDMP+
Sbjct: 181 SSRPLRDDNSLAIVWRDEGGDNSNVNRMTLKELREKVTLVANALDARFSKGDAIAIDMPM 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TV AV+IYLAIVLAG VVVSIADSF+ANEVAVRLRIS AKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVHAVIIYLAIVLAGFVVVSIADSFSANEVAVRLRISNAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNIL 360
           VIE GSCKAIVIPATGN LEVDLREQDLSW+EF+STAKE PRSNCYS +YHSIDA+TNIL
Sbjct: 301 VIEGGSCKAIVIPATGNTLEVDLREQDLSWEEFLSTAKELPRSNCYSAVYHSIDAMTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAG 420
           FSSGTTG+PKAIPWTQLSPIR+ AD+WAH+D+  GD+LCWPTNLGWV+GP S+YSC L G
Sbjct: 361 FSSGTTGDPKAIPWTQLSPIRAVADAWAHIDIHVGDILCWPTNLGWVVGPTSLYSCLLTG 420

Query: 421 ATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLD 480
           ATLALYHGSPLGRGFGKFV+             DA VTLLGTVPSLVKTWKDT+C+EGLD
Sbjct: 421 ATLALYHGSPLGRGFGKFVE-------------DAGVTLLGTVPSLVKTWKDTQCMEGLD 480

Query: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFST 540
           WTKIR+F STGETSNVDDDLWLSSRSYYKPI+E CGGTELASSYI GSPLQPQAFGAFST
Sbjct: 481 WTKIRYFTSTGETSNVDDDLWLSSRSYYKPIIECCGGTELASSYIMGSPLQPQAFGAFST 540

Query: 541 ASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQ 600
           ASMTTGFVILDEHG+PYPED PC+GE+GLFPLYLGAS+ LLNA+HDEVYFKGMP+YN MQ
Sbjct: 541 ASMTTGFVILDEHGMPYPEDQPCVGELGLFPLYLGASDTLLNADHDEVYFKGMPIYNEMQ 600

Query: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPI 660
           LRRHGDIIKR+VGGYFVVQGRADDTMNLGGIKTSSVEIERICDH +ESILETAAV VSPI
Sbjct: 601 LRRHGDIIKRTVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHAEESILETAAVGVSPI 660

Query: 661 EGGPEQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720
           EGGPEQL+ILVVLRKGY+R PQELK+KFSKAIQS LNPLFKVGFVKIVP+FPRTASNKLL
Sbjct: 661 EGGPEQLVILVVLRKGYRRSPQELKIKFSKAIQSKLNPLFKVGFVKIVPNFPRTASNKLL 720

Query: 721 RRVLRKQMKDELSLQSQL 739
           RRVLRKQMKDEL+++SQL
Sbjct: 721 RRVLRKQMKDELAVRSQL 725

BLAST of Lsi10G009660 vs. NCBI nr
Match: XP_038902724.1 (probable acyl-activating enzyme 18, peroxisomal [Benincasa hispida])

HSP 1 Score: 1355.1 bits (3506), Expect = 0.0e+00
Identity = 669/738 (90.65%), Postives = 700/738 (94.85%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           MGK+IWELGIED VKAGLNLDEA++FNRILQNAVSTA+RSDP EVWRELMAR+ALKPT+P
Sbjct: 1   MGKSIWELGIEDFVKAGLNLDEAVEFNRILQNAVSTAQRSDPAEVWRELMARRALKPTYP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           Y LHRLLYYSVYANWN+SSNGPP YWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YELHRLLYYSVYANWNVSSNGPPPYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           FSLFQKFS QNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNG+WLPDSILNIAECCLLP
Sbjct: 121 FSLFQKFSSQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGKWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SSRPLKDDNHLA+VWRDEGCD++N+N MTLKELRE+VMLVANALDATFSKGDAIAIDMPL
Sbjct: 181 SSRPLKDDNHLAVVWRDEGCDNSNVNRMTLKELRERVMLVANALDATFSKGDAIAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TV AVVIYLAIVL+GLVVVSIADSFAANEVA+RLRISKAKG+FTQDFILRGGKKYPLYSR
Sbjct: 241 TVHAVVIYLAIVLSGLVVVSIADSFAANEVAIRLRISKAKGVFTQDFILRGGKKYPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNIL 360
           VIE GSCKAIVIPATGNRLEVDLRE DLSWQEF+STAKEFPRSNCYSPIYHSIDAVTNIL
Sbjct: 301 VIEGGSCKAIVIPATGNRLEVDLREHDLSWQEFLSTAKEFPRSNCYSPIYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAG 420
           FSSGTTGEPKA+ WTQLSPIRSAADSWAH+DVQAGDV+CWPTNLGWVMGPVSVYS  L+G
Sbjct: 361 FSSGTTGEPKAVLWTQLSPIRSAADSWAHMDVQAGDVICWPTNLGWVMGPVSVYSSLLSG 420

Query: 421 ATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLD 480
           ATLALYHGSPLGRGFGKFVQ             DA VTLLGTVPSLVKTWKDT C+EGLD
Sbjct: 421 ATLALYHGSPLGRGFGKFVQ-------------DAGVTLLGTVPSLVKTWKDTSCMEGLD 480

Query: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFST 540
           WTKIR FASTGETSNVDDDLWLSSRSYYKP+LE CGGTELAS YI GSPLQPQAFGAFST
Sbjct: 481 WTKIRRFASTGETSNVDDDLWLSSRSYYKPVLECCGGTELASCYIMGSPLQPQAFGAFST 540

Query: 541 ASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQ 600
            SMTTGFVILDE+GIPYPED PCIGEIGLFPL LGAS+ LLNA+H EVYFKGMP YNGMQ
Sbjct: 541 PSMTTGFVILDEYGIPYPEDQPCIGEIGLFPLCLGASSELLNADHHEVYFKGMPKYNGMQ 600

Query: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPI 660
           LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIE+IC+HVDESILETAAVSVSPI
Sbjct: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIEQICNHVDESILETAAVSVSPI 660

Query: 661 EGGPEQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720
           EGGPE+L+ILVVLRKGYKR PQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL
Sbjct: 661 EGGPERLVILVVLRKGYKRSPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720

Query: 721 RRVLRKQMKDELSLQSQL 739
           RRVLRKQMKDELSL+SQL
Sbjct: 721 RRVLRKQMKDELSLRSQL 725

BLAST of Lsi10G009660 vs. NCBI nr
Match: XP_004142170.3 (probable acyl-activating enzyme 18, peroxisomal [Cucumis sativus])

HSP 1 Score: 1331.6 bits (3445), Expect = 0.0e+00
Identity = 655/738 (88.75%), Postives = 691/738 (93.63%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           MGK++WEL IEDLVK GLNL EA++FNRILQN +STA+RS PTEVWRELMAR+ALKPTHP
Sbjct: 1   MGKSVWELDIEDLVKTGLNLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           Y LH LLYYSVYANWNISSNGPP YWFPS++QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           FSLFQK S+Q+PEIYWSIVL ELSVTFQK PRCILDRTEKSN RWLPDSILNIAECCLLP
Sbjct: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SSRPLKDDN  AIVWR+EGCD++N+NHMTLK+LREKV LVANALDATFSKGDAIAIDMPL
Sbjct: 181 SSRPLKDDNSSAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNIL 360
           VI+ GSCKAIVIPATGNRL+VDLREQDLSW+EF+STAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAG 420
           FSSGTTGEPKAIPWTQLSPIR AADSWAH+DVQ GDV CWPTNLGWVMGPVSVYS  LAG
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420

Query: 421 ATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLD 480
           ATLALYHGSPLG GFGKFVQ             DA VTLLGTVPSLVKTWKDTRC+EGL+
Sbjct: 421 ATLALYHGSPLGHGFGKFVQ-------------DAGVTLLGTVPSLVKTWKDTRCMEGLN 480

Query: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFST 540
           WTKIRHFASTGETSNVDDDLWLSSRSYYKPI EFCGGTELASSYI G+PLQPQAFGAF+T
Sbjct: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPIFEFCGGTELASSYINGTPLQPQAFGAFTT 540

Query: 541 ASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQ 600
           ASMTT FVILDEHGIPYPED PCIGEIGLFP+YLGASN LLNA+HD+VYFKGMP+YNGMQ
Sbjct: 541 ASMTTEFVILDEHGIPYPEDQPCIGEIGLFPIYLGASNELLNADHDKVYFKGMPIYNGMQ 600

Query: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPI 660
           LRRHGDIIKR+VGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVS+SPI
Sbjct: 601 LRRHGDIIKRTVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSLSPI 660

Query: 661 EGGPEQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720
           EGGPEQL+ILVVLRK Y+R PQELKVKFSKAIQ NLNPLFKVGFVKIVPSFPRTASNKLL
Sbjct: 661 EGGPEQLVILVVLRKEYERSPQELKVKFSKAIQKNLNPLFKVGFVKIVPSFPRTASNKLL 720

Query: 721 RRVLRKQMKDELSLQSQL 739
           RRVLRKQMKDEL+L+SQL
Sbjct: 721 RRVLRKQMKDELALRSQL 725

BLAST of Lsi10G009660 vs. NCBI nr
Match: XP_031740080.1 (probable acyl-activating enzyme 18, peroxisomal [Cucumis sativus])

HSP 1 Score: 1322.8 bits (3422), Expect = 0.0e+00
Identity = 646/738 (87.53%), Postives = 689/738 (93.36%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           MGK+IWELGIEDLVK GL L EA++FNRILQN +STA+RS PTEVWRELMAR+ALKPTHP
Sbjct: 24  MGKSIWELGIEDLVKTGLKLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 83

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           Y LHRLLYYSVYANWNISSNGPP YWFPS+DQSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 84  YELHRLLYYSVYANWNISSNGPPPYWFPSIDQSKLTNLGKIMELHGPKLLGASYKDPISS 143

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           FSLFQKFS QNPEIYWSIVL+ELS+TFQKAPRCILDRTEK NGRWLPDS+LNIAECCL  
Sbjct: 144 FSLFQKFSIQNPEIYWSIVLQELSITFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 203

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDN LAIVWRDEGCD++N+NHMTLK+LREKV LVANALDATFSKGD +AIDMPL
Sbjct: 204 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDVVAIDMPL 263

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 264 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 323

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNIL 360
           VIE GSCKAIVIPA+GNRLEVDLREQDLSW+EFISTAKEFPRSNCYSP+YHSIDA+TNIL
Sbjct: 324 VIEGGSCKAIVIPASGNRLEVDLREQDLSWEEFISTAKEFPRSNCYSPMYHSIDAMTNIL 383

Query: 361 FSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAG 420
           FSSGTTGEPKAIPWTQ SPIR AADSWAH+DVQ GDV CWPTNLGW MGPVSVYS FL G
Sbjct: 384 FSSGTTGEPKAIPWTQRSPIRCAADSWAHMDVQPGDVFCWPTNLGWAMGPVSVYSAFLTG 443

Query: 421 ATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLD 480
           ATLALYHGSPLG GFGKFVQ             DA VT+LGTVPSLVKTWKDTRC+EGL+
Sbjct: 444 ATLALYHGSPLGYGFGKFVQ-------------DAGVTMLGTVPSLVKTWKDTRCMEGLN 503

Query: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFST 540
           WTKIR+FASTGETSNVDDDLWLSSRSYYKP+ E CGGTEL SS+I GSP+QPQAFGAFST
Sbjct: 504 WTKIRYFASTGETSNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFST 563

Query: 541 ASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQ 600
           ASMTTGFVILDEHGIP P+D PCIGEIGLFP YLGAS+ LLNA+HDEVYFKGMP+YNGMQ
Sbjct: 564 ASMTTGFVILDEHGIPSPDDQPCIGEIGLFPTYLGASSELLNADHDEVYFKGMPIYNGMQ 623

Query: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPI 660
           LRRHGDIIKR+VGGY VVQGRADDTMNLGGIKTSSVE+ERICDHVDES+LETAAV+VSPI
Sbjct: 624 LRRHGDIIKRTVGGYLVVQGRADDTMNLGGIKTSSVELERICDHVDESLLETAAVAVSPI 683

Query: 661 EGGPEQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720
           +GGPEQL+ILVVLR+GYK+ PQELK+KFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL
Sbjct: 684 QGGPEQLVILVVLREGYKKSPQELKIKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 743

Query: 721 RRVLRKQMKDELSLQSQL 739
           RRVLRKQMKDE++LQSQL
Sbjct: 744 RRVLRKQMKDEIALQSQL 748

BLAST of Lsi10G009660 vs. NCBI nr
Match: XP_008449821.2 (PREDICTED: probable acyl-activating enzyme 18, peroxisomal [Cucumis melo] >KAA0040067.1 putative acyl-activating enzyme 18, peroxisomal [Cucumis melo var. makuwa] >TYK21692.1 putative acyl-activating enzyme 18, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1319.7 bits (3414), Expect = 0.0e+00
Identity = 646/738 (87.53%), Postives = 689/738 (93.36%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           MGK+IWELGIEDLVK GLNL EA++FNRIL+N +STA+RS P EVWRELMA++ALKPTHP
Sbjct: 1   MGKSIWELGIEDLVKTGLNLHEAVEFNRILKNVLSTAQRSKPAEVWRELMAKRALKPTHP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           + LHRLLYYSVYANWNIS+NGPP YWFPSL+QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  FELHRLLYYSVYANWNISTNGPPPYWFPSLNQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           FSLFQKFS QNPEIYWSIVL+ELSVTFQKAPRCILDRTEK NGRWLPDS+LNIAECCL  
Sbjct: 121 FSLFQKFSIQNPEIYWSIVLQELSVTFQKAPRCILDRTEKPNGRWLPDSMLNIAECCLRS 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SSR L+DDN LAIVWRDEGCD++N+NHMTLK+LR+KV LVANALDATFSKGDA+AIDMPL
Sbjct: 181 SSRLLRDDNSLAIVWRDEGCDNSNVNHMTLKQLRDKVTLVANALDATFSKGDAVAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRL ISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLLISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNIL 360
           VIE GSCKAIVIP TGNRLEVDLREQDLSW+EF+STAKEFPRSNCYSP+YHSIDAVTNIL
Sbjct: 301 VIEGGSCKAIVIPVTGNRLEVDLREQDLSWEEFLSTAKEFPRSNCYSPMYHSIDAVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAG 420
           FSSGTTGEPKAIPWTQLSPIR AADSWAH+DVQAGDV CWPTNLGW MGPVSVYS  L G
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQAGDVFCWPTNLGWAMGPVSVYSSLLVG 420

Query: 421 ATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLD 480
           ATLALYHGSPLG GFGKFVQ             DA VT+LGTVPSLVKTWKDTRC+EGL+
Sbjct: 421 ATLALYHGSPLGHGFGKFVQ-------------DAGVTILGTVPSLVKTWKDTRCMEGLN 480

Query: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFST 540
           WTKIR+FASTGETSNVDDDLWLSSRSYYKP+ E CGGTEL SS+I GSPLQPQAFGAFST
Sbjct: 481 WTKIRYFASTGETSNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPLQPQAFGAFST 540

Query: 541 ASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQ 600
           ASMTTGFVILDEHGIP+PED PCIGEIGLFP+YLGASN LLNA+HDEVYFKGMP+YNGMQ
Sbjct: 541 ASMTTGFVILDEHGIPFPEDQPCIGEIGLFPIYLGASNELLNADHDEVYFKGMPIYNGMQ 600

Query: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPI 660
           LRRHGDIIKR+VGGY VVQGRADDTMNLGGIKTSSVE+ERICD VDESILETAAV+VSPI
Sbjct: 601 LRRHGDIIKRTVGGYLVVQGRADDTMNLGGIKTSSVELERICDLVDESILETAAVAVSPI 660

Query: 661 EGGPEQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720
           +GGPEQL+ILVVL +GY++ PQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL
Sbjct: 661 QGGPEQLVILVVLSEGYRKSPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720

Query: 721 RRVLRKQMKDELSLQSQL 739
           RRVLRKQMKDEL+LQS L
Sbjct: 721 RRVLRKQMKDELALQSHL 725

BLAST of Lsi10G009660 vs. NCBI nr
Match: KAE8649475.1 (hypothetical protein Csa_018177 [Cucumis sativus])

HSP 1 Score: 1318.5 bits (3411), Expect = 0.0e+00
Identity = 645/738 (87.40%), Postives = 689/738 (93.36%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           MGK++WEL IEDLVK GLNL EA++FNRILQN +STA+RS PTEVWRELMAR+ALKPTHP
Sbjct: 1   MGKSVWELDIEDLVKTGLNLHEAVEFNRILQNVLSTAQRSKPTEVWRELMARRALKPTHP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           Y LH LLYYSVYANWNISSNGPP YWFPS++QSKLTNLGKIMELHGPKLLGASYKDPISS
Sbjct: 61  YELHHLLYYSVYANWNISSNGPPPYWFPSINQSKLTNLGKIMELHGPKLLGASYKDPISS 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           FSLFQK S+Q+PEIYWSIVL ELSVTFQK PRCILDRTEKSN RWLPDSILNIAECCLLP
Sbjct: 121 FSLFQKLSYQSPEIYWSIVLHELSVTFQKPPRCILDRTEKSNVRWLPDSILNIAECCLLP 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SSRPLKDDN  AIVWR+EGCD++N+NHMTLK+LREKV LVANALDATFSKGDAIAIDMPL
Sbjct: 181 SSRPLKDDNSSAIVWRNEGCDNSNVNHMTLKQLREKVTLVANALDATFSKGDAIAIDMPL 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR
Sbjct: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVTNIL 360
           VI+ GSCKAIVIPATGNRL+VDLREQDLSW+EF+STAKEFPRSN YSP+YHS D VTNIL
Sbjct: 301 VIQGGSCKAIVIPATGNRLDVDLREQDLSWEEFLSTAKEFPRSNRYSPVYHSSDGVTNIL 360

Query: 361 FSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLAG 420
           FSSGTTGEPKAIPWTQLSPIR AADSWAH+DVQ GDV CWPTNLGWVMGPVSVYS  LAG
Sbjct: 361 FSSGTTGEPKAIPWTQLSPIRCAADSWAHMDVQPGDVFCWPTNLGWVMGPVSVYSSLLAG 420

Query: 421 ATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGLD 480
           ATLALYHGSPLG GFGKFVQ             DA VT+LGTVPSLVKTWKDTRC+EGL+
Sbjct: 421 ATLALYHGSPLGHGFGKFVQ-------------DAGVTMLGTVPSLVKTWKDTRCMEGLN 480

Query: 481 WTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFST 540
           WTKIR+FASTGETSNVDDDLWLSSRSYYKP+ E CGGTEL SS+I GSP+QPQAFGAFST
Sbjct: 481 WTKIRYFASTGETSNVDDDLWLSSRSYYKPLFECCGGTELGSSFIVGSPVQPQAFGAFST 540

Query: 541 ASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGMQ 600
           ASMTTGFVILDEHGIP P+D PCIGEIGLFP YLGAS+ LLNA+HDEVYFKGMP+YNGMQ
Sbjct: 541 ASMTTGFVILDEHGIPSPDDQPCIGEIGLFPTYLGASSELLNADHDEVYFKGMPIYNGMQ 600

Query: 601 LRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSPI 660
           LRRHGDIIKR+VGGY VVQGRADDTMNLGGIKTSSVE+ERICDHVDES+LETAAV+VSPI
Sbjct: 601 LRRHGDIIKRTVGGYLVVQGRADDTMNLGGIKTSSVELERICDHVDESLLETAAVAVSPI 660

Query: 661 EGGPEQLIILVVLRKGYKRFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720
           +GGPEQL+ILVVLR+GYK+ PQELK+KFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL
Sbjct: 661 QGGPEQLVILVVLREGYKKSPQELKIKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNKLL 720

Query: 721 RRVLRKQMKDELSLQSQL 739
           RRVLRKQMKDE++LQSQL
Sbjct: 721 RRVLRKQMKDEIALQSQL 725

BLAST of Lsi10G009660 vs. TAIR 10
Match: AT1G55320.1 (acyl-activating enzyme 18 )

HSP 1 Score: 1030.0 bits (2662), Expect = 9.2e-301
Identity = 493/741 (66.53%), Postives = 605/741 (81.65%), Query Frame = 0

Query: 1   MGKNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHP 60
           M K+I EL  +D VKAGL L++A +F++++ + ++ A  +DP + W+ L+    LKP HP
Sbjct: 1   MWKSIGELSCDDYVKAGLTLEDAKEFDKLVSDVITKAIETDPRDQWKALVDESVLKPWHP 60

Query: 61  YGLHRLLYYSVYANWNISSNGPPLYWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISS 120
           + LH+LLYYSVY+NW+ S +GPPLYWFPSL QSK TNLGK+ME HGP+LLG SYK+P+ S
Sbjct: 61  HPLHQLLYYSVYSNWDSSVHGPPLYWFPSLSQSKSTNLGKLMEYHGPRLLGPSYKNPLES 120

Query: 121 FSLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNGRWLPDSILNIAECCLLP 180
           F LF++FS ++PE+YWS V++ELS+ F   PRCIL+++ K  G WLPD++LNIAECCL+P
Sbjct: 121 FELFRRFSVEHPEVYWSFVIDELSLVFHTPPRCILNKS-KPEGTWLPDAVLNIAECCLMP 180

Query: 181 SSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDATFSKGDAIAIDMPL 240
           SS P K+D+ +A+VWR+EG DD+ +N MT+KELRE+VMLVANA+  +F KGD IAIDMP+
Sbjct: 181 SSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAISGSFEKGDTIAIDMPM 240

Query: 241 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 300
           TVDAV+IYLAI+LAG +VVSIADSFAA E+A RL+ISKAKGIFTQD+ILRGG+++PLYSR
Sbjct: 241 TVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFTQDYILRGGRRFPLYSR 300

Query: 301 VIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPR-SNCYSPIYHSIDAVTNI 360
           V+E    K IV+PA+G  L V LREQD+SW +F+S AK      N Y PIY  +++V NI
Sbjct: 301 VVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNAKPHSSGENYYRPIYLPVESVINI 360

Query: 361 LFSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCFLA 420
           LFSSGTTGEPKAIPWTQLSPIRSA D WAHLDVQ G   CWPTNLGWVMGP  ++SCFL 
Sbjct: 361 LFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGHTYCWPTNLGWVMGPTLMFSCFLT 420

Query: 421 GATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLEGL 480
           GATLALY GSPLGRGFGKFVQ             DA VT+LGTVPSLVKTWK T C+EGL
Sbjct: 421 GATLALYSGSPLGRGFGKFVQ-------------DAGVTVLGTVPSLVKTWKRTNCMEGL 480

Query: 481 DWTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGAFS 540
           +WTKI+ FA+TGE SNVDD LWLSS++ YKP++E CGGTELASSYI GSPLQPQAFGAFS
Sbjct: 481 NWTKIKFFATTGEASNVDDVLWLSSKADYKPVIECCGGTELASSYIIGSPLQPQAFGAFS 540

Query: 541 TASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYNGM 600
           T SMTT  +I DE+G+PYP+D PC GE+GLFP +LGA++RLLNANHDEVYFKGMP+Y   
Sbjct: 541 TPSMTTRIIIFDENGVPYPDDQPCTGEVGLFPQHLGATDRLLNANHDEVYFKGMPMYKET 600

Query: 601 QLRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSVSP 660
           +LRRHGDI+KR+VGGY+ VQGRADDTMNLGGIKTSS+EIER+CD  DE I ETAAV+++P
Sbjct: 601 RLRRHGDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQADECISETAAVTLTP 660

Query: 661 IEGGPEQLIILVVLRKGYK-RFPQELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTASNK 720
             GGPE L+I  VL++G+K +  +ELK+KFS+ IQ +LNPLFKV FVKIVP FPRTAS+K
Sbjct: 661 PNGGPELLVIFAVLKEGFKQQSGEELKMKFSRTIQKDLNPLFKVSFVKIVPEFPRTASSK 720

Query: 721 LLRRVLRKQMKDE-LSLQSQL 739
           LLRRVLR Q+K E LSL+S++
Sbjct: 721 LLRRVLRDQIKQELLSLRSRI 727

BLAST of Lsi10G009660 vs. TAIR 10
Match: AT1G55320.2 (acyl-activating enzyme 18 )

HSP 1 Score: 750.4 bits (1936), Expect = 1.4e-216
Identity = 369/524 (70.42%), Postives = 433/524 (82.63%), Query Frame = 0

Query: 218 MLVANALDATFSKGDAIAIDMPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRIS 277
           MLVANA+  +F KGD IAIDMP+TVDAV+IYLAI+LAG +VVSIADSFAA E+A RL+IS
Sbjct: 1   MLVANAISGSFEKGDTIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKIS 60

Query: 278 KAKGIFTQDFILRGGKKYPLYSRVIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTA 337
           KAKGIFTQD+ILRGG+++PLYSRV+E    K IV+PA+G  L V LREQD+SW +F+S A
Sbjct: 61  KAKGIFTQDYILRGGRRFPLYSRVVEAAPSKVIVLPASGTELHVQLREQDVSWMDFLSNA 120

Query: 338 KEFPR-SNCYSPIYHSIDAVTNILFSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGD 397
           K      N Y PIY  +++V NILFSSGTTGEPKAIPWTQLSPIRSA D WAHLDVQ G 
Sbjct: 121 KPHSSGENYYRPIYLPVESVINILFSSGTTGEPKAIPWTQLSPIRSACDGWAHLDVQVGH 180

Query: 398 VLCWPTNLGWVMGPVSVYSCFLAGATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAE 457
             CWPTNLGWVMGP  ++SCFL GATLALY GSPLGRGFGKFVQ             DA 
Sbjct: 181 TYCWPTNLGWVMGPTLMFSCFLTGATLALYSGSPLGRGFGKFVQ-------------DAG 240

Query: 458 VTLLGTVPSLVKTWKDTRCLEGLDWTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCG 517
           VT+LGTVPSLVKTWK T C+EGL+WTKI+ FA+TGE SNVDD LWLSS++ YKP++E CG
Sbjct: 241 VTVLGTVPSLVKTWKRTNCMEGLNWTKIKFFATTGEASNVDDVLWLSSKADYKPVIECCG 300

Query: 518 GTELASSYITGSPLQPQAFGAFSTASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGA 577
           GTELASSYI GSPLQPQAFGAFST SMTT  +I DE+G+PYP+D PC GE+GLFP +LGA
Sbjct: 301 GTELASSYIIGSPLQPQAFGAFSTPSMTTRIIIFDENGVPYPDDQPCTGEVGLFPQHLGA 360

Query: 578 SNRLLNANHDEVYFKGMPVYNGMQLRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSV 637
           ++RLLNANHDEVYFKGMP+Y   +LRRHGDI+KR+VGGY+ VQGRADDTMNLGGIKTSS+
Sbjct: 361 TDRLLNANHDEVYFKGMPMYKETRLRRHGDIVKRTVGGYYNVQGRADDTMNLGGIKTSSI 420

Query: 638 EIERICDHVDESILETAAVSVSPIEGGPEQLIILVVLRKGYK-RFPQELKVKFSKAIQSN 697
           EIER+CD  DE I ETAAV+++P  GGPE L+I  VL++G+K +  +ELK+KFS+ IQ +
Sbjct: 421 EIERVCDQADECISETAAVTLTPPNGGPELLVIFAVLKEGFKQQSGEELKMKFSRTIQKD 480

Query: 698 LNPLFKVGFVKIVPSFPRTASNKLLRRVLRKQMKDE-LSLQSQL 739
           LNPLFKV FVKIVP FPRTAS+KLLRRVLR Q+K E LSL+S++
Sbjct: 481 LNPLFKVSFVKIVPEFPRTASSKLLRRVLRDQIKQELLSLRSRI 511

BLAST of Lsi10G009660 vs. TAIR 10
Match: AT5G23050.1 (acyl-activating enzyme 17 )

HSP 1 Score: 708.8 bits (1828), Expect = 4.7e-204
Identity = 370/743 (49.80%), Postives = 494/743 (66.49%), Query Frame = 0

Query: 3   KNIWELGIEDLVKAGLNLDEALKFNRILQNAVSTAKRSDPTEVWRELMARKALKPTHPYG 62
           K++  +   D+   G++ D + K  R L++ +  +  S P E W ++ +R+ L P  P+ 
Sbjct: 4   KSLNSITKSDIEALGISGDVSEKLLRDLEDIIHGS--STPPETWIQI-SRRILHPNLPFS 63

Query: 63  LHRLLYYSVYANWNISSNGPPL-YWFPSLDQSKLTNLGKIMELHGPKLLGASYKDPISSF 122
            H+++YY  Y ++     GP L  W P    + LTN+GK++E  G + LG +YK+P+SSF
Sbjct: 64  FHQMMYYGCYKDF-----GPDLPAWIPDPKVASLTNVGKLLEKRGKEFLGGNYKNPVSSF 123

Query: 123 SLFQKFSFQNPEIYWSIVLEELSVTFQKAPRCILDRT---EKSNGRWLPDSILNIAECCL 182
           S FQ+FS  NPE+YW  VL+EL++ F   P+CIL++    +   G+WLP + LN A  CL
Sbjct: 124 SSFQEFSVSNPEVYWKTVLDELNILFSVPPKCILEKDTSGDNPGGKWLPGAYLNPARNCL 183

Query: 183 LPSSRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANALDAT-FSKGDAIAID 242
               +   DD  + I WRDEG DD  +N MTL ELR +V L A+AL A    +  AIA+D
Sbjct: 184 TNGFKRRLDD--IVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALGLEEESAIAVD 243

Query: 243 MPLTVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPL 302
           MP+ V++V+IYLAIVLAG VVVSIADSF+  E++ RL+ISKAK IFTQD I+RG K  PL
Sbjct: 244 MPMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDVIIRGDKSIPL 303

Query: 303 YSRVIERGSCKAIVIPATGNRLEVDLREQDLSWQEFISTAKEFPRSNCYSPIYHSIDAVT 362
           Y RV++  +  AIV+PA G+   + LRE+DLSW  F+  A+   R   Y        A T
Sbjct: 304 YRRVVDAEAPLAIVVPARGSSCRMKLREKDLSWNNFLGNARNL-RGVEYVAAEKPAGAYT 363

Query: 363 NILFSSGTTGEPKAIPWTQLSPIRSAADSWAHLDVQAGDVLCWPTNLGWVMGPVSVYSCF 422
           NILFSSGTTGEPKAIPWT +SP++SAAD+W HLDVQ GDV+ WPTNLGW+MGP  VY+  
Sbjct: 364 NILFSSGTTGEPKAIPWTNISPLKSAADAWCHLDVQRGDVVAWPTNLGWMMGPWLVYASL 423

Query: 423 LAGATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDAEVTLLGTVPSLVKTWKDTRCLE 482
           + GA + LY+GSPLG  F KFVQ             DAEV++LG +PS+V+TW+++    
Sbjct: 424 INGACMGLYNGSPLGPTFAKFVQ-------------DAEVSVLGVIPSIVRTWQNSNSTS 483

Query: 483 GLDWTKIRHFASTGETSNVDDDLWLSSRSYYKPILEFCGGTELASSYITGSPLQPQAFGA 542
           G DW++IR F STGE SN+D+ LWL  R++YKP++E+CGGTE+  S+I+GS LQPQ+  A
Sbjct: 484 GYDWSRIRCFGSTGEASNIDEYLWLMGRAHYKPVIEYCGGTEIGGSFISGSLLQPQSLAA 543

Query: 543 FSTASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYLGASNRLLNANHDEVYFKGMPVYN 602
           FSTA+M     ILDE   P P     +GE+ L P   GAS+ LLN NH +VYF+GMP + 
Sbjct: 544 FSTAAMGCKLFILDEDSNPIPPYAAGVGELALCPHMFGASSTLLNGNHFKVYFQGMPTFQ 603

Query: 603 GMQLRRHGDIIKRSVGGYFVVQGRADDTMNLGGIKTSSVEIERICDHVDESILETAAVSV 662
           G  LRRHGD+ +R+  GY+   GRADDTMNLGGIK  S+EIER+C+ VD+S+LETAA+ V
Sbjct: 604 GQILRRHGDLFERTSKGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGV 663

Query: 663 SPIEGGPEQLIILVVLRKGYKRFP--QELKVKFSKAIQSNLNPLFKVGFVKIVPSFPRTA 722
            P  GGPEQL+I VV +      P    LK  F+  IQ  LNPLFKV  V  +PS PRTA
Sbjct: 664 PPPSGGPEQLVIAVVFKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTA 721

Query: 723 SNKLLRRVLRKQMKDELSLQSQL 739
           +NK++RRVLR+Q+  +  L S+L
Sbjct: 724 TNKVMRRVLRQQL-TQTGLNSKL 721

BLAST of Lsi10G009660 vs. TAIR 10
Match: AT5G36880.1 (acetyl-CoA synthetase )

HSP 1 Score: 120.6 bits (301), Expect = 5.5e-27
Identity = 158/634 (24.92%), Postives = 262/634 (41.32%), Query Frame = 0

Query: 126 KFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNG----RWLPDSILNIAECCLLPS 185
           K S  +P  +WS +  E     +   +   +  +   G     W    I NI   CL  +
Sbjct: 65  KRSMDDPAAFWSDIASEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYNCLDKN 124

Query: 186 SRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANAL-DATFSKGDAIAIDMPL 245
                 D   AI W  EG +      +T  EL ++V  +AN L D    KGDA+ I +P+
Sbjct: 125 VEAGLGDK-TAIHW--EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPM 184

Query: 246 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 305
            ++  +  LA    G V   +   F+A+ +A R+   K   I T + + RG K   L + 
Sbjct: 185 LMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAI 244

Query: 306 V---IERGSCK--AIVIPATGNRLEVDLRE-------QDLSWQEFIS---TAKEFPRSNC 365
           V   +++ S    ++ I  T +      RE       +D+ WQ+ IS   T+ E    + 
Sbjct: 245 VDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEWVDA 304

Query: 366 YSPIYHSIDAVTNILFSSGTTGEPKAIPWTQLSPIRSAADSWAH-LDVQAGDVLCWPTNL 425
             P++        +L++SG+TG+PK +  T    +   A ++ +  D ++ DV     + 
Sbjct: 305 EDPLF--------LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADC 364

Query: 426 GWVMGPVSV-YSCFLAGATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDA-EVTLLGT 485
           GW+ G   V Y   L GAT+ ++ G+P               P R  ++ D  +V++  T
Sbjct: 365 GWITGHSYVTYGPMLNGATVVVFEGAP-----------NYPDPGRCWDIVDKYKVSIFYT 424

Query: 486 VPSLVKTW--KDTRCLEGLDWTKIRHFASTGETSNVDDDLWL-----SSRSYYKPILEFC 545
            P+LV++    D + +       +R   S GE  N     W       SR    PI +  
Sbjct: 425 APTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRC---PISDTW 484

Query: 546 GGTELASSYITGSP-LQPQAFGAFSTASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYL 605
             TE     IT  P   PQ  G+ +        VI+DE G     +  C G + +   + 
Sbjct: 485 WQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEI--EGECSGYLCVKGSWP 544

Query: 606 GASNRLL--NANHDEVYFKGMPVYNGMQLRRHGDIIKRSVGGYFVVQGRADDTMNLGGIK 665
           GA   L   +  ++  YFK    Y        GD   R   GY+ + GR DD +N+ G +
Sbjct: 545 GAFRTLFGDHERYETTYFKPFAGY-----YFSGDGCSRDKDGYYWLTGRVDDVINVSGHR 604

Query: 666 TSSVEIERICDHVDESILETAAVSVSPIEGGPEQLIILVVLRKGYKRFPQELKVKFSKAI 725
             + E+E     +     E A V +     G + +   V L +G   + +EL+      +
Sbjct: 605 IGTAEVESAL-VLHPQCAEAAVVGIEHEVKG-QGIYAFVTLLEGVP-YSEELRKSLVLMV 663

Query: 726 QSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRK 727
           ++ +        +   P  P+T S K++RR+LRK
Sbjct: 665 RNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 663

BLAST of Lsi10G009660 vs. TAIR 10
Match: AT5G36880.2 (acetyl-CoA synthetase )

HSP 1 Score: 120.6 bits (301), Expect = 5.5e-27
Identity = 158/634 (24.92%), Postives = 262/634 (41.32%), Query Frame = 0

Query: 126 KFSFQNPEIYWSIVLEELSVTFQKAPRCILDRTEKSNG----RWLPDSILNIAECCLLPS 185
           K S  +P  +WS +  E     +   +   +  +   G     W    I NI   CL  +
Sbjct: 115 KRSMDDPAAFWSDIASEFYWKQKWGDQVFSENLDVRKGPISIEWFKGGITNICYNCLDKN 174

Query: 186 SRPLKDDNHLAIVWRDEGCDDANMNHMTLKELREKVMLVANAL-DATFSKGDAIAIDMPL 245
                 D   AI W  EG +      +T  EL ++V  +AN L D    KGDA+ I +P+
Sbjct: 175 VEAGLGDK-TAIHW--EGNELGVDASLTYSELLQRVCQLANYLKDNGVKKGDAVVIYLPM 234

Query: 246 TVDAVVIYLAIVLAGLVVVSIADSFAANEVAVRLRISKAKGIFTQDFILRGGKKYPLYSR 305
            ++  +  LA    G V   +   F+A+ +A R+   K   I T + + RG K   L + 
Sbjct: 235 LMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNVILTCNAVKRGPKTINLKAI 294

Query: 306 V---IERGSCK--AIVIPATGNRLEVDLRE-------QDLSWQEFIS---TAKEFPRSNC 365
           V   +++ S    ++ I  T +      RE       +D+ WQ+ IS   T+ E    + 
Sbjct: 295 VDAALDQSSKDGVSVGICLTYDNSLATTRENTKWQNGRDVWWQDVISQYPTSCEVEWVDA 354

Query: 366 YSPIYHSIDAVTNILFSSGTTGEPKAIPWTQLSPIRSAADSWAH-LDVQAGDVLCWPTNL 425
             P++        +L++SG+TG+PK +  T    +   A ++ +  D ++ DV     + 
Sbjct: 355 EDPLF--------LLYTSGSTGKPKGVLHTTGGYMIYTATTFKYAFDYKSTDVYWCTADC 414

Query: 426 GWVMGPVSV-YSCFLAGATLALYHGSPLGRGFGKFVQGQMGCPMRLVEMHDA-EVTLLGT 485
           GW+ G   V Y   L GAT+ ++ G+P               P R  ++ D  +V++  T
Sbjct: 415 GWITGHSYVTYGPMLNGATVVVFEGAP-----------NYPDPGRCWDIVDKYKVSIFYT 474

Query: 486 VPSLVKTW--KDTRCLEGLDWTKIRHFASTGETSNVDDDLWL-----SSRSYYKPILEFC 545
            P+LV++    D + +       +R   S GE  N     W       SR    PI +  
Sbjct: 475 APTLVRSLMRDDDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVGDSRC---PISDTW 534

Query: 546 GGTELASSYITGSP-LQPQAFGAFSTASMTTGFVILDEHGIPYPEDHPCIGEIGLFPLYL 605
             TE     IT  P   PQ  G+ +        VI+DE G     +  C G + +   + 
Sbjct: 535 WQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEI--EGECSGYLCVKGSWP 594

Query: 606 GASNRLL--NANHDEVYFKGMPVYNGMQLRRHGDIIKRSVGGYFVVQGRADDTMNLGGIK 665
           GA   L   +  ++  YFK    Y        GD   R   GY+ + GR DD +N+ G +
Sbjct: 595 GAFRTLFGDHERYETTYFKPFAGY-----YFSGDGCSRDKDGYYWLTGRVDDVINVSGHR 654

Query: 666 TSSVEIERICDHVDESILETAAVSVSPIEGGPEQLIILVVLRKGYKRFPQELKVKFSKAI 725
             + E+E     +     E A V +     G + +   V L +G   + +EL+      +
Sbjct: 655 IGTAEVESAL-VLHPQCAEAAVVGIEHEVKG-QGIYAFVTLLEGVP-YSEELRKSLVLMV 713

Query: 726 QSNLNPLFKVGFVKIVPSFPRTASNKLLRRVLRK 727
           ++ +        +   P  P+T S K++RR+LRK
Sbjct: 715 RNQIGAFAAPDRIHWAPGLPKTRSGKIMRRILRK 713