Lsi08G003420 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G003420
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	rRNA N-glycosidase
Location	chr08: 9334554 .. 9336188 (+)
RNA-Seq Expression	Lsi08G003420
Synteny	Lsi08G003420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAATTATAGTAGCTTTCATTGTAGCACTTTGTCTTGCCACTGATGGCATTCTTGCAGCAAATCTTCATTTATCCAAGAGACCCGTGATTGATGTTTACAAAAGCTTTATCCAAACCGTTCGTAAAAAATTTGGGTCCAAAACCCATAATCTCTATGGCATTCCAATCTTGCAACACTCTTTATCGAATTCAGATCGTTTCTATCTCATTGATACCGGTAACGTAGCTGGCGATACCATCACTCTTGCAGTAGATGCAGAAGATATGAGTGTCGTTGCATATCTTGCTGGTGATAATGATTCTTACTTTTTTGCAAATGCTCCACAAATAGCCTTTGATATTCTCTTCTCTAATACCCATAAAACCCTTTTGAGTTTTGAAAATACCTTCCAATCCATTGAAAAGGCAGCTAGGACTACAAGACAAGCAACTCCCCTTGGCCTTGAATCTACCAACTCTGCCATCTCAAACTTGTTTCATTACCGTCCTCATCTTGCCCCTGTTTCGTTTCTTGTCGTCTTTCAAATGATCTTCGAAGCTGCAAAATTCAAATTCGTCGAACAAGCCATTGTTAACAGCATAAAGAATGGAGAAAGCTTCACACCCAACCTTGCAATTGTGAGCTTAGAGGACAATTGGTCAGAACTTTCATTACAAATCCAAGCATCAACTTCATTACAAGGACTATTTGGCAGTAGTATCATGCTTTATAACTCTAGAAATGAAGTTATTGAAGTGGATAGTATATATTATCCAATCATACTTCCTAATCTTGCTTTGCAATTATATCATTGCAATACACAAGATTACATCAAGATGCCTGGCGTTGTCGTTCAACCTGAGAATTCTCGATGTTACGTCAAAGAACGAACAACGCGGATTAGCGGTCGAGATGGATTTTGTGCAGATGTAGCTAATGCGTATTTTAATGATGGTTCTCGTGTTATTTTGTTCCCATGTAGACAACAAGTCAATCAACAATGGACGTTCCATAGCGATCACACGATTCGATCCTTAGGAAAATGTTTGATCTCGAATAAATCCTCATCTGAAAATCCTCTGGCTGTGATTTACAATTGTAGTAAAATCAGCCAACAATATGCTACGTGGGATGTTTCTATTAGTGGGACAATCATGAACTCAGCTAATGATCTTGTCTTGACTTCAAATAAAGGAAGCAACGACAACTTGATAATGGAGAATAATATATATGGTGGTAGTCAAGGGTGGAGAGTTGGAAATTATGTGGATCCAATTATAACTTCAATTATAGGAGTTAATCAAATGTGTTTGGAGGCTAAGGATGAAAACACAAACATTTGGTTGGAAGAATGTGTGAAGAACAAAAGAGAGCAATCTTGGGCGATTTATAGTGATGGAAGTATTAGAGTAAGCAATAATCAAAGCCTTTGTGTGACTGCTTCTTCAATTGACTCATATAAGCTAATTGTCATTCTTAACTGCAATGGCTTAGCTACTCAACGTTGGGTTTTCAAGACAGATGGCACAATCTCACCTCCAAAAAATGAGAACTTGGTGATGGATATCGCAGAAAATAATGTTGATCTCAAGAGAATTATTTTGTATCCTCGACATGGTTTGGTTAACCAACAATGGGTGGTTTTCTACTAA

mRNA sequence

ATGAGAATTATAGTAGCTTTCATTGTAGCACTTTGTCTTGCCACTGATGGCATTCTTGCAGCAAATCTTCATTTATCCAAGAGACCCGTGATTGATGTTTACAAAAGCTTTATCCAAACCGTTCGTAAAAAATTTGGGTCCAAAACCCATAATCTCTATGGCATTCCAATCTTGCAACACTCTTTATCGAATTCAGATCGTTTCTATCTCATTGATACCGGTAACGTAGCTGGCGATACCATCACTCTTGCAGTAGATGCAGAAGATATGAGTGTCGTTGCATATCTTGCTGGTGATAATGATTCTTACTTTTTTGCAAATGCTCCACAAATAGCCTTTGATATTCTCTTCTCTAATACCCATAAAACCCTTTTGAGTTTTGAAAATACCTTCCAATCCATTGAAAAGGCAGCTAGGACTACAAGACAAGCAACTCCCCTTGGCCTTGAATCTACCAACTCTGCCATCTCAAACTTGTTTCATTACCGTCCTCATCTTGCCCCTGTTTCGTTTCTTGTCGTCTTTCAAATGATCTTCGAAGCTGCAAAATTCAAATTCGTCGAACAAGCCATTGTTAACAGCATAAAGAATGGAGAAAGCTTCACACCCAACCTTGCAATTGTGAGCTTAGAGGACAATTGGTCAGAACTTTCATTACAAATCCAAGCATCAACTTCATTACAAGGACTATTTGGCAGTAGTATCATGCTTTATAACTCTAGAAATGAAGTTATTGAAGTGGATAGTATATATTATCCAATCATACTTCCTAATCTTGCTTTGCAATTATATCATTGCAATACACAAGATTACATCAAGATGCCTGGCGTTGTCGTTCAACCTGAGAATTCTCGATGTTACGTCAAAGAACGAACAACGCGGATTAGCGGTCGAGATGGATTTTGTGCAGATGTAGCTAATGCGTATTTTAATGATGGTTCTCGTGTTATTTTGTTCCCATGTAGACAACAAGTCAATCAACAATGGACGTTCCATAGCGATCACACGATTCGATCCTTAGGAAAATGTTTGATCTCGAATAAATCCTCATCTGAAAATCCTCTGGCTGTGATTTACAATTGTAGTAAAATCAGCCAACAATATGCTACGTGGGATGTTTCTATTAGTGGGACAATCATGAACTCAGCTAATGATCTTGTCTTGACTTCAAATAAAGGAAGCAACGACAACTTGATAATGGAGAATAATATATATGGTGGTAGTCAAGGGTGGAGAGTTGGAAATTATGTGGATCCAATTATAACTTCAATTATAGGAGTTAATCAAATGTGTTTGGAGGCTAAGGATGAAAACACAAACATTTGGTTGGAAGAATGTGTGAAGAACAAAAGAGAGCAATCTTGGGCGATTTATAGTGATGGAAGTATTAGAGTAAGCAATAATCAAAGCCTTTGTGTGACTGCTTCTTCAATTGACTCATATAAGCTAATTGTCATTCTTAACTGCAATGGCTTAGCTACTCAACGTTGGGTTTTCAAGACAGATGGCACAATCTCACCTCCAAAAAATGAGAACTTGGTGATGGATATCGCAGAAAATAATGTTGATCTCAAGAGAATTATTTTGTATCCTCGACATGGTTTGGTTAACCAACAATGGGTGGTTTTCTACTAA

Coding sequence (CDS)

ATGAGAATTATAGTAGCTTTCATTGTAGCACTTTGTCTTGCCACTGATGGCATTCTTGCAGCAAATCTTCATTTATCCAAGAGACCCGTGATTGATGTTTACAAAAGCTTTATCCAAACCGTTCGTAAAAAATTTGGGTCCAAAACCCATAATCTCTATGGCATTCCAATCTTGCAACACTCTTTATCGAATTCAGATCGTTTCTATCTCATTGATACCGGTAACGTAGCTGGCGATACCATCACTCTTGCAGTAGATGCAGAAGATATGAGTGTCGTTGCATATCTTGCTGGTGATAATGATTCTTACTTTTTTGCAAATGCTCCACAAATAGCCTTTGATATTCTCTTCTCTAATACCCATAAAACCCTTTTGAGTTTTGAAAATACCTTCCAATCCATTGAAAAGGCAGCTAGGACTACAAGACAAGCAACTCCCCTTGGCCTTGAATCTACCAACTCTGCCATCTCAAACTTGTTTCATTACCGTCCTCATCTTGCCCCTGTTTCGTTTCTTGTCGTCTTTCAAATGATCTTCGAAGCTGCAAAATTCAAATTCGTCGAACAAGCCATTGTTAACAGCATAAAGAATGGAGAAAGCTTCACACCCAACCTTGCAATTGTGAGCTTAGAGGACAATTGGTCAGAACTTTCATTACAAATCCAAGCATCAACTTCATTACAAGGACTATTTGGCAGTAGTATCATGCTTTATAACTCTAGAAATGAAGTTATTGAAGTGGATAGTATATATTATCCAATCATACTTCCTAATCTTGCTTTGCAATTATATCATTGCAATACACAAGATTACATCAAGATGCCTGGCGTTGTCGTTCAACCTGAGAATTCTCGATGTTACGTCAAAGAACGAACAACGCGGATTAGCGGTCGAGATGGATTTTGTGCAGATGTAGCTAATGCGTATTTTAATGATGGTTCTCGTGTTATTTTGTTCCCATGTAGACAACAAGTCAATCAACAATGGACGTTCCATAGCGATCACACGATTCGATCCTTAGGAAAATGTTTGATCTCGAATAAATCCTCATCTGAAAATCCTCTGGCTGTGATTTACAATTGTAGTAAAATCAGCCAACAATATGCTACGTGGGATGTTTCTATTAGTGGGACAATCATGAACTCAGCTAATGATCTTGTCTTGACTTCAAATAAAGGAAGCAACGACAACTTGATAATGGAGAATAATATATATGGTGGTAGTCAAGGGTGGAGAGTTGGAAATTATGTGGATCCAATTATAACTTCAATTATAGGAGTTAATCAAATGTGTTTGGAGGCTAAGGATGAAAACACAAACATTTGGTTGGAAGAATGTGTGAAGAACAAAAGAGAGCAATCTTGGGCGATTTATAGTGATGGAAGTATTAGAGTAAGCAATAATCAAAGCCTTTGTGTGACTGCTTCTTCAATTGACTCATATAAGCTAATTGTCATTCTTAACTGCAATGGCTTAGCTACTCAACGTTGGGTTTTCAAGACAGATGGCACAATCTCACCTCCAAAAAATGAGAACTTGGTGATGGATATCGCAGAAAATAATGTTGATCTCAAGAGAATTATTTTGTATCCTCGACATGGTTTGGTTAACCAACAATGGGTGGTTTTCTACTAA

Protein sequence

MRIIVAFIVALCLATDGILAANLHLSKRPVIDVYKSFIQTVRKKFGSKTHNLYGIPILQHSLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQIAFDILFSNTHKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQMIFEAAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIMLYNSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIKMPGVVVQPENSRCYVKERTTRISGRDGFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPLAVIYNCSKISQQYATWDVSISGTIMNSANDLVLTSNKGSNDNLIMENNIYGGSQGWRVGNYVDPIITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVTASSIDSYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHGLVNQQWVVFY

Homology

[Close]

BLAST of Lsi08G003420 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 626.7 bits (1615), Expect = 2.4e-178
Identity = 322/529 (60.87%), Postives = 405/529 (76.56%), Query Frame = 0


Query: 21  ANLHLSKRPVIDVYKSFIQTVRKKFGSKTHNLYGIPILQHSLSNSDRFYLIDTGNVAGDT 80
           ANL LS+      YK+FI  VR++ GS+T+ LYGIP+L+HSLSNS+RFYL+   +   ++
Sbjct: 4   ANLRLSEAN-SGTYKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQDES 63

Query: 81  ITLAVDAEDMSVVAYL-AGDNDSYFFANAPQIAFDILFSNTHKTLLSFENTFQSIEKAAR 140
           ITLA+D EDM  VAY  AG ++SYFF NAPQIAF  LF++TH+ +L+F+NTF+S+E AA 
Sbjct: 64  ITLAIDVEDMVAVAYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENAAG 123

Query: 141 TTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQMIFEAAKFKFVEQAIVNSIKNGE 200
           TTRQ   LG++  + AISNLF+  P L P+SFLV+ QM+ EA+KF+F+EQ++  S KN +
Sbjct: 124 TTRQTIVLGVDPLDFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKNEK 183

Query: 201 SFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIMLYNSRNEVIEVDSIYYPIILPNL 260
           +F P+LAIVSLEDNWSE+SLQIQASTSLQGLFGS + LYNS NE+IEVDSIYYPIIL N+
Sbjct: 184 TFLPDLAIVSLEDNWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILANV 243

Query: 261 ALQLYHCNTQDYIKMPGVVVQPENSRCYVKERTTRISGRDGFCADVANAYFNDGSRVILF 320
           ALQLYHC            V   ++ C V+ RTTRISGRD  C DVA A  +DGSR+IL+
Sbjct: 244 ALQLYHCQ-----------VSTGDNECLVETRTTRISGRDALCVDVAGALTSDGSRLILY 303

Query: 321 PCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPLAVIYNCSKISQQYATWDVSISGTI 380
           PC QQVNQ+WTFHSD T+RSLGKCL +N S   N L VIY+CSK++ +  +WDVS+ GTI
Sbjct: 304 PCGQQVNQKWTFHSDGTVRSLGKCLATNNSKFGN-LVVIYDCSKLAAEDISWDVSVGGTI 363

Query: 381 MN-SANDLVLTSNKGS-NDNLIMENNIYGGSQGWRVGNYVDPIITSIIGVNQMCLEAKDE 440
           MN +  DL LTSNK + + NL ME N Y  SQGWRVGNYV PII SI+G++ MCLEA D 
Sbjct: 364 MNPNYEDLALTSNKATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDG 423

Query: 441 NTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVTASS--IDSYKLIVILNCNGLATQ 500
           NTN+WLEECV N+REQSWA+YSDG+IRV +N+ LCVTASS   D++K+I ILNC+G   Q
Sbjct: 424 NTNMWLEECVPNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSNNQ 483

Query: 501 RWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHGLVNQQWVVFY 545
           RWVF  DG+IS P N+ L MD+A ++VDLK+IIL+  HG +NQQWV+FY
Sbjct: 484 RWVFLADGSISTPGNQRLAMDVARSDVDLKKIILHRPHGDLNQQWVLFY 519

[Close]

BLAST of Lsi08G003420 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 372.9 bits (956), Expect = 6.4e-102
Identity = 213/565 (37.70%), Postives = 336/565 (59.47%), Query Frame = 0


Query: 1   MRIIVA-----FIVALCLATDGI-------LAANLHLSKRPVIDVYKSFIQTVRKKFGSK 60
           MR++ A     +IV L + + GI       ++ NL  +K      Y+ F+  +RK   + 
Sbjct: 1   MRVVAAAMLYFYIVVLAICSVGIQGIDYPSVSFNLDGAKSA---TYRDFLSNLRKTVATG 60

Query: 61  THNLYGIPIL--QHSLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFA 120
           T+ + G+P+L  +  +    RF L+   N  G+T+TLAVD  ++ VVA+ +G+ +SYFF 
Sbjct: 61  TYEVNGLPVLRRESEVQVKSRFVLVPLTNYNGNTVTLAVDVTNLYVVAF-SGNANSYFFK 120

Query: 121 NAPQIAFDILFSNTHKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHL 180
           +A ++    LF  T +  LSF   + ++E AA T R++  LG    + AI++L+H     
Sbjct: 121 DATEVQKSNLFVGTKQNTLSFTGNYDNLETAANTRRESIELGPSPLDGAITSLYH--GDS 180

Query: 181 APVSFLVVFQMIFEAAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTS 240
              S LVV QM+ EAA+F+++EQ +  S++   SFTPN  ++S+E+NWS +SL+IQ + +
Sbjct: 181 VARSLLVVIQMVSEAARFRYIEQEVRRSLQQATSFTPNALMLSMENNWSSMSLEIQQAGN 240

Query: 241 LQGLFGSSIMLYNSRNEVIEVDSIYYPIILPNLALQLYHCNT---QDYIKMPGVVVQPEN 300
               F  ++ L N  +    VD+      +  +A+ L+ C++    + I+MP  +   +N
Sbjct: 241 NVSPFFGTVQLLNYDHTHRLVDNFEELYKITGIAILLFRCSSPSNDNAIRMPLDLAGEDN 300

Query: 301 -----SRCYVKERTTR-ISGRDGFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTI 360
                  C ++   TR I GRDG C DV N Y  DG+ + L+PC  Q NQ+WTF SD TI
Sbjct: 301 KYNDGETCTLRTSFTRNIVGRDGLCVDVRNGYDTDGTPLQLWPCGTQRNQRWTFDSDDTI 360

Query: 361 RSLGKCLISNKSSSENPLAVIYNCSKISQQYATWDVSISGTIMNSANDLVLTSNK-GSND 420
           RS+GKC+ +N  ++ + + VI+NCS  ++    W+V I G+I+N ++ LV+T+ +  S  
Sbjct: 361 RSMGKCMTANGLNNGSNI-VIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRT 420

Query: 421 NLIMENNIYGGSQGWRVGNYVDPIITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWA 480
            L++E+NIY  SQGW V N V PI+ SI+G  +MCL++  EN  +W+E+C     +Q WA
Sbjct: 421 ILLLEDNIYAASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWA 480

Query: 481 IYSDGSIRVSNNQSLCVTASSIDSYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMD 540
           +Y D +IRV++ + LCVT +  +S  LI+IL C GL +QRW F +DG I  PK+ + VMD
Sbjct: 481 LYGDRTIRVNSTRGLCVTTNGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRH-VMD 540

Query: 541 IAENNVDLKRIILYPRHGLVNQQWV 542
           +  +NV L+ II++P  G  NQQWV
Sbjct: 541 VRASNVSLREIIIFPATGNPNQQWV 557

[Close]

BLAST of Lsi08G003420 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 9.2e-101
Identity = 226/574 (39.37%), Postives = 326/574 (56.79%), Query Frame = 0


Query: 1   MRII--VAFIVALCLATDGILAANLHLSKRPVI-----------DVYKSFIQTVRKKFGS 60
           MR++  + ++V L +   GI  A  H    P +           D Y  F++ +++K   
Sbjct: 1   MRVVTKLLYLVVLAICGLGIHGALTHTRVTPPVYPSVSFNLTGADTYGPFLRALQEKVIL 60

Query: 61  KTHNLYGIPIL--QHSLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFF 120
             H  + +P+L  +  +S+S+RF L+   N +GDT+TLA+D  ++ VVA+ + +  SYFF
Sbjct: 61  GNHTAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVNLYVVAF-SSNGRSYFF 120

Query: 121 ANAPQIAFDILFSNTHKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPH 180
           + +  +  D LF +T +  L+F   + S+E+     R   PLG +S   AIS+L  Y   
Sbjct: 121 SGSTAVQRDNLFVDTTQEELNFTGNYISLERQVGFGRVYIPLGPKSLAQAISSLRTYTLS 180

Query: 181 LAPV-----SFLVVFQMIFEAAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQ 240
                      LVV QM+ EAA+F+++E  I  SI +   FTP+L ++S+E+NWS +S +
Sbjct: 181 AGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPDLLMLSMENNWSSMSSE 240

Query: 241 IQASTSLQGLFGSSIMLYNSRNEVIEVDSIYYPIILPNLALQLYHC----------NTQD 300
           IQ +    G+F   + L + RN  IEV +      L  +A+ LY C          N  D
Sbjct: 241 IQQAQP-GGIFPGVVQLRDERNNPIEVTNFRRLFELTYIAVLLYGCAPVTSNSYTNNAID 300

Query: 301 --YIKMPGVVVQPENSRCYVKERTTRISGRDGFCADVANAYFNDGSRVILFPCRQQVNQQ 360
              IKMP          C V E T RISG DG C DV + ++ DG+ V L PC  + NQ 
Sbjct: 301 AQIIKMPVFRGGGYEKVCSVVEVTRRISGWDGLCVDVRDGHYIDGNTVQLGPCGNECNQL 360

Query: 361 WTFHSDHTIRSLGKCLISNKSSSENPLAVIYNCSKISQQYATWDVSISGTIMNSANDLVL 420
           WTF +D TIR LGKCL ++ S       +IY+C+ +  +   W VS  GTI N  + LVL
Sbjct: 361 WTFRTDGTIRWLGKCLTTSSS------VMIYDCNTVPPEATKWVVSTDGTITNPRSGLVL 420

Query: 421 TSNKGSNDN-LIMENNIYGGSQGWRVGNYVDPIITSIIGVNQMCLEAKDENTNIWLEECV 480
           T+ + +    L +ENNI+   QGW VG+ V+P++T I+G  QMCL    EN  +WLE+CV
Sbjct: 421 TAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMCLTENGENNFVWLEDCV 480

Query: 481 KNKREQSWAIYSDGSIRVSNNQSLCVTASSIDSYKLIVILNCNGLATQRWVFKTDGTISP 540
            N+ EQ WA+Y DG+IRV++N+SLCVT+   +   LIVIL C G   QRWVF T+GTIS 
Sbjct: 481 LNRVEQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISN 540

Query: 541 PKNENLVMDIAENNVDLKRIILYPRHGLVNQQWV 542
           P N  LVMD+A++NV L++IILYP  G  NQQW+
Sbjct: 541 P-NAKLVMDVAQSNVSLRKIILYPPTGNPNQQWI 564

[Close]

BLAST of Lsi08G003420 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 6.6e-99
Identity = 222/574 (38.68%), Postives = 325/574 (56.62%), Query Frame = 0


Query: 1   MRII--VAFIVALCLATDGILAANLHLSKRPVI-----------DVYKSFIQTVRKKFGS 60
           MR++  + ++  L +   GI  A  H    P +           D Y+ F++ +++K   
Sbjct: 1   MRLVAKLLYLAVLAICGLGIHGALTHPRVTPPVYPSVSFNLTGADTYEPFLRALQEKVIL 60

Query: 61  KTHNLYGIPIL--QHSLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFF 120
             H  + +P+L  +  +S+S+RF L+   N +GDT+TLA+D  ++ VVA+ + +  SYFF
Sbjct: 61  GNHTAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVNLYVVAF-SSNGKSYFF 120

Query: 121 ANAPQIAFDILFSNTHKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPH 180
           + +  +  D LF +T +  L+F   + S+E+     R   PLG +S + AIS+L  Y   
Sbjct: 121 SGSTAVQRDNLFVDTTQEELNFTGNYTSLERQVGFGRVYIPLGPKSLDQAISSLRTYTLT 180

Query: 181 LAPV-----SFLVVFQMIFEAAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQ 240
                      LVV QM+ EAA+F+++E  I  SI +   FTP+L ++S+E+NWS +S +
Sbjct: 181 AGDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPDLLMLSMENNWSSMSSE 240

Query: 241 IQASTSLQGLFGSSIMLYNSRNEVIEVDSIYYPIILPNLALQLYHC----------NTQD 300
           IQ +    G+F   + L + RN  IEV +      L  +A+ LY C          N  D
Sbjct: 241 IQQAQP-GGIFAGVVQLRDERNNSIEVTNFRRLFELTYIAVLLYGCAPVTSSSYSNNAID 300

Query: 301 --YIKMPGVVVQPENSRCYVKERTTRISGRDGFCADVANAYFNDGSRVILFPCRQQVNQQ 360
              IKMP          C V E T RISG DG C DV   ++ DG+ V L PC  + NQ 
Sbjct: 301 AQIIKMPVFRGGEYEKVCSVVEVTRRISGWDGLCVDVRYGHYIDGNPVQLRPCGNECNQL 360

Query: 361 WTFHSDHTIRSLGKCLISNKSSSENPLAVIYNCSKISQQYATWDVSISGTIMNSANDLVL 420
           WTF +D TIR LGKCL ++ S       +IY+C+ +  +   W VSI GTI N  + LVL
Sbjct: 361 WTFRTDGTIRWLGKCLTASSS------VMIYDCNTVPPEATKWVVSIDGTITNPHSGLVL 420

Query: 421 TSNKGSNDN-LIMENNIYGGSQGWRVGNYVDPIITSIIGVNQMCLEAKDENTNIWLEECV 480
           T+ + +    L +ENNI+   QGW VG+ V+P++T I+G  QMCL    EN  +WLE+CV
Sbjct: 421 TAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMCLRENGENNFVWLEDCV 480

Query: 481 KNKREQSWAIYSDGSIRVSNNQSLCVTASSIDSYKLIVILNCNGLATQRWVFKTDGTISP 540
            N+ +Q WA+Y DG+IRV++N+SLCVT+   +   LIVIL C G   QRWVF T+GTIS 
Sbjct: 481 LNRVQQEWALYGDGTIRVNSNRSLCVTSEDHEPSDLIVILKCEGSGNQRWVFNTNGTISN 540

Query: 541 PKNENLVMDIAENNVDLKRIILYPRHGLVNQQWV 542
           P N  L+MD+A+ +V L++IILY   G  NQQW+
Sbjct: 541 P-NAKLLMDVAQRDVSLRKIILYRPTGNPNQQWI 564

[Close]

BLAST of Lsi08G003420 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.9e-93
Identity = 217/569 (38.14%), Postives = 324/569 (56.94%), Query Frame = 0


Query: 5   VAFIVALCLATDGILAANLHLSKRPVI-------------DVYKSFIQTVRKKFGSKTHN 64
           + ++V L +   GI  A+   S  P +             + Y+ F+Q +R K    +H 
Sbjct: 7   ILYLVVLAICGLGIHGAHPTHSAPPTVYPSVSFNLTEANSNEYRHFLQELRGKVILGSHR 66

Query: 65  LYGIPIL--QHSLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAP 124
            + +P+L  +  +S+SDRF L+   N +   +TLA+D     VVA+   D  SYFF+ + 
Sbjct: 67  AFDLPVLNPESKVSDSDRFVLVRLTNPSRKKVTLAIDVVTFYVVAFAQNDR-SYFFSGSS 126

Query: 125 QIAFDILFSNTHKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLA-- 184
           ++  + LF +T +  L+F+  + S+E      R   PLG +S   +IS+L  Y+      
Sbjct: 127 EVQRENLFVDTTQEDLNFKGDYTSLEHQVGFGRVYIPLGPKSLAQSISSLSTYKSSAGDN 186

Query: 185 ---PVSFLVVFQMIFEAAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQAS 244
                S LVV QM+ EAA+F++++  I  SI + + FTP+L ++S+E+ WS +S +IQ +
Sbjct: 187 KRLARSLLVVIQMVSEAARFRYIQLRIQASITDAKEFTPDLLMLSMENKWSSMSSEIQQA 246

Query: 245 TSLQGLFGSSIMLYNSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIKMPGVVVQ---- 304
               G F   + L + RN  I+V +      L ++A+ L+ C T    KMP  +++    
Sbjct: 247 QP-GGAFAQVVKLLDQRNHPIDVTNFRRLFQLTSVAVLLHGCPT--VTKMPAYIIKMPVF 306

Query: 305 ---PENSRC-YVKERTTRISGRDGFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHT 364
               +  RC  V+E T RI GRDGFCA+V N    DG+ V L  C +Q NQQWTF +D T
Sbjct: 307 NGGEDEERCSVVEEVTRRIGGRDGFCAEVKNGDEKDGTPVQLSSCGEQSNQQWTFSTDGT 366

Query: 365 IRSLGKCLISNKSSSENPLAVIYNCSKISQQYATWDVSISGTIMNSANDLVLTSNKGSND 424
           I+SLGKCL ++ S       +IYNC  +  +   W VSI GTI N  + LVLT+ K +  
Sbjct: 367 IQSLGKCLTTSSS------VMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTAPKAAEG 426

Query: 425 NLI-MENNIYGGSQGWRVGNYVDPIITSIIGVNQMCLEAKDENTNIWLEEC-VK--NKRE 484
            L+ +E N++   QGW VGN V+P++T I+G  QMCLE    N ++ L +C VK  +K +
Sbjct: 427 TLVSLEKNVHAARQGWIVGN-VEPLVTFIVGYEQMCLETNPGNNDVSLGDCSVKSASKVD 486

Query: 485 QSWAIYSDGSIRVSNNQSLCVTASSIDSYKLIVILNCNGLATQRWVFKTDGTISPPKNEN 542
           Q WA+Y DG+IRV+N++SLCVT+    S + I+IL C G A QRWVF TDGTIS P +  
Sbjct: 487 QKWALYGDGTIRVNNDRSLCVTSEGKSSNEPIIILKCLGWANQRWVFNTDGTISNP-DSK 546

[Close]

BLAST of Lsi08G003420 vs. ExPASy TrEMBL
Match: A0A0A0KN71 (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 3.4e-223
Identity = 395/546 (72.34%), Postives = 457/546 (83.70%), Query Frame = 0


Query: 1   MRIIVAFIVALCLATDGILAANLHLSKRPVIDVYKSFIQTVRKKFGSKTHNLYGIPILQH 60
           MR+++AFIV L +AT+        LSKRPV+DVY+S+IQT+R+KFGSKTH LYGIP+L H
Sbjct: 1   MRVLLAFIVTLSIATNA-------LSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHH 60

Query: 61  SLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQIAFDILFSNT 120
           SLSNSDRF+LID GN  GDTIT AVDA+DMSVVAYLAGDNDSYFF+NAP+ AFDILF  T
Sbjct: 61  SLSNSDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKT 120

Query: 121 HKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQMIFE 180
           ++ LL+F+N+F+SIE AA TTR+ATPLGL+ +N+AI+NLFHY P LAPVSFL+VFQM+FE
Sbjct: 121 NQNLLNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFE 180

Query: 181 AAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIMLYNS 240
           +AKFKF+EQ IVNSI NGE+FTP+LA++SLEDNWSELSL+IQAS SLQGLFGSS+ LYNS
Sbjct: 181 SAKFKFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNS 240

Query: 241 RNEVIEVDSIYYPIILPNLALQLYHCNTQDYIKMP-GVVVQPENSRCYVKERTTRISGRD 300
           +NE +EVDSIYYP+IL NLALQLYHCN QDYIKMP   V   +N RCYVKERT RISG+D
Sbjct: 241 KNEAVEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQD 300

Query: 301 GFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPLAVIY 360
           G CADVA     DGS VI  PC QQ NQQWTFH DHTIRS  KCLI NKS + NPLAVI 
Sbjct: 301 GLCADVA----RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKA-NPLAVIQ 360

Query: 361 NCSKISQQYATWDVSISGTIMNSANDLVLTSNKGSNDN-LIMENNIYGGSQGWRVGNYVD 420
           NC+K+SQ+ ATWDVSISGTIMN A DLVLTS  G NDN L M+ N Y G+QGWRVGNYV+
Sbjct: 361 NCNKVSQEDATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVE 420

Query: 421 PIITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVTASSI 480
           PII SIIGV QMCLEA +ENTNIWLEECVKNK EQSWA++SDGSIRV+N+ SLC+TASSI
Sbjct: 421 PIIASIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSI 480

Query: 481 DSYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHGLVNQ 540
           +S + IVI  CNGLA+QRWV K DGTIS PK E LVMD+A++NVDLK I+LYPR  LV+Q
Sbjct: 481 ESKQRIVIAKCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQ 534

Query: 541 QWVVFY 545
            WV  Y
Sbjct: 541 HWVALY 534

[Close]

BLAST of Lsi08G003420 vs. ExPASy TrEMBL
Match: A0A5A7V3Q8 (rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G00010 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.9e-221
Identity = 394/546 (72.16%), Postives = 457/546 (83.70%), Query Frame = 0


Query: 1   MRIIVAFIVALCLATDGILAANLHLSKRPVIDVYKSFIQTVRKKFGSKTHNLYGIPILQH 60
           MR+++AF+V L  AT+        LSKRPV+DVY+S+IQT+R+KFGSKTH LYGIP+LQH
Sbjct: 1   MRVLLAFVVTLSFATNA-------LSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLQH 60

Query: 61  SLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQIAFDILFSNT 120
           SLSNSDRF+LIDTGN  GDTITLAVDA+DMSVVAYLAG++DSYFF+NAP+ AFDILF NT
Sbjct: 61  SLSNSDRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFPNT 120

Query: 121 HKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQMIFE 180
           ++ LL+F+++F+SIEKAA TTRQATPLG + +N+AI+NLFHY   LAPVSFL+VFQMIFE
Sbjct: 121 NQNLLNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMIFE 180

Query: 181 AAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIMLYNS 240
           +AKF F+EQ IVNSIK GE+FTP+LA++SLEDNWSELSLQIQAS SLQGLFGSS+ LYNS
Sbjct: 181 SAKFNFIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLYNS 240

Query: 241 RNEVIEVDSIYYPIILPNLALQLYHCNTQDYIKMP-GVVVQPENSRCYVKERTTRISGRD 300
           +NE IEVDSIYYP+IL NLALQLYHCN QDYIKMP   V   +N RCYVKERT RISGRD
Sbjct: 241 KNEAIEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISGRD 300

Query: 301 GFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPLAVIY 360
           G C DVA     DGS+VI  PC QQ NQ+WTF+ DHTIRS  KCLI  KS + NP AVI 
Sbjct: 301 GLCVDVA----RDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKSKA-NPFAVIL 360

Query: 361 NCSKISQQYATWDVSISGTIMNSANDLVLTSNKGSNDN-LIMENNIYGGSQGWRVGNYVD 420
           +C+K+SQQ ATWDVSISGTIMN A DLVLTSN G NDN L M+ N Y G+QGWRVGN+V+
Sbjct: 361 DCNKVSQQDATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVE 420

Query: 421 PIITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVTASSI 480
           P+I SIIGV QMCLEA +ENTNIWLEECVKNK EQSWA+YSDGSIRV+N+ SLCVTASSI
Sbjct: 421 PLIVSIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTASSI 480

Query: 481 DSYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHGLVNQ 540
           +  + IVI  CNGLA+QRWV   DGTIS PKNE LVMD+A++NVDLK I+LYPR  LV+Q
Sbjct: 481 EPKQRIVIGKCNGLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLVSQ 534

Query: 541 QWVVFY 545
           QWV  Y
Sbjct: 541 QWVALY 534

[Close]

BLAST of Lsi08G003420 vs. ExPASy TrEMBL
Match: A0A1S3CIM5 (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103501401 PE=3 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.9e-221
Identity = 394/546 (72.16%), Postives = 457/546 (83.70%), Query Frame = 0


Query: 1   MRIIVAFIVALCLATDGILAANLHLSKRPVIDVYKSFIQTVRKKFGSKTHNLYGIPILQH 60
           MR+++AF+V L  AT+        LSKRPV+DVY+S+IQT+R+KFGSKTH LYGIP+LQH
Sbjct: 1   MRVLLAFVVTLSFATNA-------LSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLQH 60

Query: 61  SLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQIAFDILFSNT 120
           SLSNSDRF+LIDTGN  GDTITLAVDA+DMSVVAYLAG++DSYFF+NAP+ AFDILF NT
Sbjct: 61  SLSNSDRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFPNT 120

Query: 121 HKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQMIFE 180
           ++ LL+F+++F+SIEKAA TTRQATPLG + +N+AI+NLFHY   LAPVSFL+VFQMIFE
Sbjct: 121 NQNLLNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMIFE 180

Query: 181 AAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIMLYNS 240
           +AKF F+EQ IVNSIK GE+FTP+LA++SLEDNWSELSLQIQAS SLQGLFGSS+ LYNS
Sbjct: 181 SAKFNFIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLYNS 240

Query: 241 RNEVIEVDSIYYPIILPNLALQLYHCNTQDYIKMP-GVVVQPENSRCYVKERTTRISGRD 300
           +NE IEVDSIYYP+IL NLALQLYHCN QDYIKMP   V   +N RCYVKERT RISGRD
Sbjct: 241 KNEAIEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISGRD 300

Query: 301 GFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPLAVIY 360
           G C DVA     DGS+VI  PC QQ NQ+WTF+ DHTIRS  KCLI  KS + NP AVI 
Sbjct: 301 GLCVDVA----RDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKSKA-NPFAVIL 360

Query: 361 NCSKISQQYATWDVSISGTIMNSANDLVLTSNKGSNDN-LIMENNIYGGSQGWRVGNYVD 420
           +C+K+SQQ ATWDVSISGTIMN A DLVLTSN G NDN L M+ N Y G+QGWRVGN+V+
Sbjct: 361 DCNKVSQQDATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVE 420

Query: 421 PIITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVTASSI 480
           P+I SIIGV QMCLEA +ENTNIWLEECVKNK EQSWA+YSDGSIRV+N+ SLCVTASSI
Sbjct: 421 PLIVSIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTASSI 480

Query: 481 DSYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHGLVNQ 540
           +  + IVI  CNGLA+QRWV   DGTIS PKNE LVMD+A++NVDLK I+LYPR  LV+Q
Sbjct: 481 EPKQRIVIGKCNGLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLVSQ 534

Query: 541 QWVVFY 545
           QWV  Y
Sbjct: 541 QWVALY 534

[Close]

BLAST of Lsi08G003420 vs. ExPASy TrEMBL
Match: B7X8M2 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MCL1 PE=1 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 2.2e-142
Identity = 284/551 (51.54%), Postives = 377/551 (68.42%), Query Frame = 0


Query: 1   MRIIVAFI-VALCLATDGILAANLHLSKRPV-IDVYKSFIQTVRKKFG-SKTHNLYGIPI 60
           MR++  +I VAL L  +GI   NL LS+     D YKSFI+ +RK+     ++   GIPI
Sbjct: 3   MRVLAVYIVVALSLTINGI-ECNLSLSQSNFSADTYKSFIKNLRKQLTIGASYGSAGIPI 62

Query: 61  LQHSLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQIAFDILF 120
           L+HS+   +RF L+D  N   +TITLA++ ED    AY A D  SYFF NAP IA  ++F
Sbjct: 63  LKHSVPICERFLLVDLTNGDNETITLAINVEDAGFAAYRAADR-SYFFQNAPPIASYVIF 122

Query: 121 SNTHKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQM 180
           ++T++ +++F NTF+SIE    TTR  TPLG+    ++I +LF +  +  P SFLV+ QM
Sbjct: 123 TDTNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVPTSFLVLIQM 182

Query: 181 IFEAAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIML 240
           + EAAKFKF+EQ +++SI + E FTP LA++SLE+NW++LSLQ+QAS SL G+FG S+ L
Sbjct: 183 VLEAAKFKFIEQKVIHSIMDMEDFTPGLAMLSLEENWTQLSLQLQASESLNGVFGDSVSL 242

Query: 241 YNSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIKMPGVVVQP--ENSRCYVKERTTRI 300
           YNS +E I VDS+YYPI+  N+A QLY C     I+M   +  P   N +C  ++RTTRI
Sbjct: 243 YNSMDEPIGVDSMYYPILTANMAFQLYQC-PYGVIRMMPTITMPNQNNEQCSPQQRTTRI 302

Query: 301 SGRDGFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPL 360
           SGRDG C DV  A   DGSRVIL+PC QQ NQQWTF+ D+TIRSLGKCL ++  SS + +
Sbjct: 303 SGRDGLCVDVYGALTADGSRVILYPCGQQQNQQWTFYPDNTIRSLGKCLATSALSSGSNV 362

Query: 361 AVIYNCSKISQQYATWDVSISGTIMNSANDLVLTSNKG-SNDNLIMENNIYGGSQGWRVG 420
            VI NC  +      W VS SGT+MN ++ LVLT+N   S  NL  ENN++   Q WR+G
Sbjct: 363 -VITNCDYLRYDDG-WMVSSSGTMMNKSSHLVLTANAATSRTNLTGENNVFAAKQAWRIG 422

Query: 421 NYVDPIITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVT 480
           NYV+PI+T+IIG+  MCLEA D +TN+WLE CVKNK +Q WA+YSD +IRV+NN++LCV+
Sbjct: 423 NYVEPIVTTIIGLRHMCLEATDNDTNVWLESCVKNKTKQYWALYSDDTIRVNNNRNLCVS 482

Query: 481 ASSIDSYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHG 540
           +S+  S KLIVI  C+G   QRWVF   GTIS P  E  VMD+A+N+V LK+I+L     
Sbjct: 483 SSTDSSSKLIVIRRCDGSINQRWVFTPQGTISNPGYE-AVMDVAQNDVYLKKIVLSSATD 542

Query: 541 LVN-QQWVVFY 545
             N QQW VFY
Sbjct: 543 KGNGQQWTVFY 547

[Close]

BLAST of Lsi08G003420 vs. ExPASy TrEMBL
Match: A0A0A0K979 (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 1.5e-138
Identity = 276/546 (50.55%), Postives = 370/546 (67.77%), Query Frame = 0


Query: 3   IIVAFIVALCLATDGILAANLHLSKRP-VIDVYKSFIQTVRKKFGSKTHNLYGIPILQHS 62
           II+AF   L LAT  I    L LS    V+D YK+FI  +R++    T  LY IPIL+ S
Sbjct: 8   IIIAF---LSLATPSIDGNQLSLSSSDLVVDGYKNFINVIRQRLTENTLKLYDIPILESS 67

Query: 63  LSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQIAFDILFSNTH 122
           L  + RF  I+ GN   +TI+LA+D  ++ VV Y   +N+SY F +AP+ A D++F  T 
Sbjct: 68  LPPTQRFMNINIGNQNDETISLAIDRVNLGVVGY-RSNNNSYIFLDAPKAALDVVFPETC 127

Query: 123 KTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQMIFEA 182
           + +L F + ++SIEKA+ TTR  T LGLE  NSAISNLFHYR    P SFLV+ QM+ E 
Sbjct: 128 RVVLGFNSDYESIEKASGTTRLQTLLGLEPLNSAISNLFHYRRADIPGSFLVILQMVLEG 187

Query: 183 AKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIMLYNSR 242
           +KFKF+EQ+++ S+K G +F P LAIVSLEDNW +LS QIQAS SLQGLFG +I LY+S 
Sbjct: 188 SKFKFIEQSVIQSLKYGYNFKPGLAIVSLEDNWDKLSSQIQASPSLQGLFGEAIRLYDSN 247

Query: 243 NEVIEVDSIYYPIILPNLALQLYHCN-TQDYIKMPGVVVQPENSRCYVKERTTRISGRDG 302
           ++ I+VDSIYY II  N+A QL+HCN + ++I+MP  V    +  C V+ RT  ISG++G
Sbjct: 248 DKFIDVDSIYYVIITTNIAFQLHHCNVSTNFIRMPSDV----DDSCNVQTRTAMISGQNG 307

Query: 303 FCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPLAVIYN 362
           FC D +     +G+ +IL+ C  Q+NQ+WTF SD TIR   KCL    S       V+YN
Sbjct: 308 FCVDSSRILDYNGNPIILYRCINQLNQEWTFLSDKTIRYSNKCLTFETSR----YVVLYN 367

Query: 363 CSKISQQ-YATWDVSISGTIMNSANDLVLTSNKGSN-DNLIMENNIYGGSQGWRVGNYVD 422
           CS++ ++    W+V+I GTI N ++ LVLT++  +N   LI+E N +  SQGWRVGNYV 
Sbjct: 368 CSEVEEKGNIRWNVAIDGTISNPSSGLVLTTDPSTNRSQLIVEVNKFTTSQGWRVGNYVK 427

Query: 423 PIITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVTASSI 482
           PII SIIG+ +MCLEA + NTN+WLE+CVKNK EQ WA+YSDGSIRV+  ++LCV++SS 
Sbjct: 428 PIIGSIIGMEEMCLEATNNNTNMWLEKCVKNKAEQYWAVYSDGSIRVNRKRNLCVSSSSN 487

Query: 483 DSYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHGLVNQ 542
            S  L++I  C G + QRW F  +GTI  P+ +  V+D+  + V  KRIILYP+ GL NQ
Sbjct: 488 RSGALLIIDECKGTSNQRWNFLANGTILNPETKK-VVDVYGSMVSAKRIILYPKTGLANQ 540

Query: 543 QWVVFY 545
           QW +FY
Sbjct: 548 QWTLFY 540

[Close]

BLAST of Lsi08G003420 vs. NCBI nr
Match: XP_038884532.1 (seed lectin-like [Benincasa hispida])

HSP 1 Score: 823.2 bits (2125), Expect = 1.4e-234
Identity = 422/545 (77.43%), Postives = 472/545 (86.61%), Query Frame = 0


Query: 1   MRIIVAFIVALCLATDGILAANLHLSKRPVIDVYKSFIQTVRKKFGSKTH-NLYGIPILQ 60
           MR++VA I+ALC+A DGI AA     K P+ D YK++IQ VR+KF S+TH  LYGIP+LQ
Sbjct: 15  MRVLVALIIALCIAIDGITAA-----KTPISDRYKTYIQNVRQKFASQTHKQLYGIPMLQ 74

Query: 61  HSLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQIAFDILFSN 120
           HSLSNSDRF LIDT NVAGDTITLAVDA+DMSVVAYLAGDNDSYFF+NAP   FDILF N
Sbjct: 75  HSLSNSDRFLLIDTANVAGDTITLAVDAQDMSVVAYLAGDNDSYFFSNAPLFTFDILFPN 134

Query: 121 THKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQMIF 180
           TH+TLLS++NTF+SIE AA TTRQA PLGL  +NSAISNLFHYRP LA VSFLVVFQMIF
Sbjct: 135 THQTLLSYDNTFESIENAANTTRQAIPLGLNPSNSAISNLFHYRPDLAAVSFLVVFQMIF 194

Query: 181 EAAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIMLYN 240
           EAAKFKF+EQ IVN IKNGESFTPNLAIVSLEDNWS+LSLQIQ+STSLQGLFGSS+ML+N
Sbjct: 195 EAAKFKFIEQTIVNCIKNGESFTPNLAIVSLEDNWSQLSLQIQSSTSLQGLFGSSVMLHN 254

Query: 241 SRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIKMPGVVVQPENSRCYVKERTTRISGRD 300
           S+NE IEVDSIY+PIIL NLALQLYHCNTQDYIK+P +V   ENSRCYV+E TTRISGRD
Sbjct: 255 SKNEAIEVDSIYFPIILNNLALQLYHCNTQDYIKLPSLV---ENSRCYVEEHTTRISGRD 314

Query: 301 GFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPLAVIY 360
           G CADVA     DGS VI FPCRQQ NQQWTF+SD TIRSLGKCLI +KSSS NPLAVIY
Sbjct: 315 GLCADVA-----DGSHVISFPCRQQANQQWTFYSDRTIRSLGKCLIPSKSSS-NPLAVIY 374

Query: 361 NCSKISQQYATWDVSISGTIMNSANDLVLTSNKGSNDNLIMENNIYGGSQGWRVGNYVDP 420
           +CSK+SQQ ATW+VS+SGTIMN+ ND VLTSN GS   L ME+N Y G+QGWRVGN+VDP
Sbjct: 375 DCSKVSQQDATWEVSLSGTIMNTYNDSVLTSNNGS--TLTMEDNAYDGNQGWRVGNFVDP 434

Query: 421 IITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVTASSID 480
           I+TSIIGV +MCLEA + NTNIWLEECVK+K EQSW +YSDGSIRV+NN SLCVTA SI 
Sbjct: 435 IVTSIIGVKEMCLEATEGNTNIWLEECVKDKIEQSWTLYSDGSIRVNNNHSLCVTA-SIH 494

Query: 481 SYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHGLVNQQ 540
           S + IVILNCNGLATQRWVFK DGTIS PK++ LVMD+A+N+VDLKRI+LYPRHG VNQQ
Sbjct: 495 SRQRIVILNCNGLATQRWVFKADGTISTPKDDGLVMDVAQNDVDLKRIVLYPRHGGVNQQ 542

Query: 541 WVVFY 545
           WVVFY
Sbjct: 555 WVVFY 542

[Close]

BLAST of Lsi08G003420 vs. NCBI nr
Match: XP_004140357.1 (seed lectin [Cucumis sativus] >KGN51070.1 hypothetical protein Csa_008446 [Cucumis sativus])

HSP 1 Score: 784.3 bits (2024), Expect = 7.0e-223
Identity = 395/546 (72.34%), Postives = 457/546 (83.70%), Query Frame = 0


Query: 1   MRIIVAFIVALCLATDGILAANLHLSKRPVIDVYKSFIQTVRKKFGSKTHNLYGIPILQH 60
           MR+++AFIV L +AT+        LSKRPV+DVY+S+IQT+R+KFGSKTH LYGIP+L H
Sbjct: 1   MRVLLAFIVTLSIATNA-------LSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLHH 60

Query: 61  SLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQIAFDILFSNT 120
           SLSNSDRF+LID GN  GDTIT AVDA+DMSVVAYLAGDNDSYFF+NAP+ AFDILF  T
Sbjct: 61  SLSNSDRFHLIDAGNEPGDTITFAVDAQDMSVVAYLAGDNDSYFFSNAPKFAFDILFPKT 120

Query: 121 HKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQMIFE 180
           ++ LL+F+N+F+SIE AA TTR+ATPLGL+ +N+AI+NLFHY P LAPVSFL+VFQM+FE
Sbjct: 121 NQNLLNFDNSFKSIEIAANTTREATPLGLKPSNAAIANLFHYDPVLAPVSFLIVFQMVFE 180

Query: 181 AAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIMLYNS 240
           +AKFKF+EQ IVNSI NGE+FTP+LA++SLEDNWSELSL+IQAS SLQGLFGSS+ LYNS
Sbjct: 181 SAKFKFIEQRIVNSITNGEAFTPDLAMLSLEDNWSELSLEIQASNSLQGLFGSSVTLYNS 240

Query: 241 RNEVIEVDSIYYPIILPNLALQLYHCNTQDYIKMP-GVVVQPENSRCYVKERTTRISGRD 300
           +NE +EVDSIYYP+IL NLALQLYHCN QDYIKMP   V   +N RCYVKERT RISG+D
Sbjct: 241 KNEAVEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVADHQNPRCYVKERTVRISGQD 300

Query: 301 GFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPLAVIY 360
           G CADVA     DGS VI  PC QQ NQQWTFH DHTIRS  KCLI NKS + NPLAVI 
Sbjct: 301 GLCADVA----RDGSHVISSPCGQQANQQWTFHRDHTIRSSDKCLIPNKSKA-NPLAVIQ 360

Query: 361 NCSKISQQYATWDVSISGTIMNSANDLVLTSNKGSNDN-LIMENNIYGGSQGWRVGNYVD 420
           NC+K+SQ+ ATWDVSISGTIMN A DLVLTS  G NDN L M+ N Y G+QGWRVGNYV+
Sbjct: 361 NCNKVSQEDATWDVSISGTIMNPAYDLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVE 420

Query: 421 PIITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVTASSI 480
           PII SIIGV QMCLEA +ENTNIWLEECVKNK EQSWA++SDGSIRV+N+ SLC+TASSI
Sbjct: 421 PIIASIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVFSDGSIRVNNDHSLCMTASSI 480

Query: 481 DSYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHGLVNQ 540
           +S + IVI  CNGLA+QRWV K DGTIS PK E LVMD+A++NVDLK I+LYPR  LV+Q
Sbjct: 481 ESKQRIVIAKCNGLASQRWVLKADGTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQ 534

Query: 541 QWVVFY 545
            WV  Y
Sbjct: 541 HWVALY 534

[Close]

BLAST of Lsi08G003420 vs. NCBI nr
Match: XP_008463192.1 (PREDICTED: nigrin b-like [Cucumis melo] >KAA0062872.1 nigrin b-like [Cucumis melo var. makuwa] >TYK22504.1 nigrin b-like [Cucumis melo var. makuwa])

HSP 1 Score: 778.5 bits (2009), Expect = 3.8e-221
Identity = 394/546 (72.16%), Postives = 457/546 (83.70%), Query Frame = 0


Query: 1   MRIIVAFIVALCLATDGILAANLHLSKRPVIDVYKSFIQTVRKKFGSKTHNLYGIPILQH 60
           MR+++AF+V L  AT+        LSKRPV+DVY+S+IQT+R+KFGSKTH LYGIP+LQH
Sbjct: 1   MRVLLAFVVTLSFATNA-------LSKRPVVDVYQSYIQTIRQKFGSKTHKLYGIPVLQH 60

Query: 61  SLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQIAFDILFSNT 120
           SLSNSDRF+LIDTGN  GDTITLAVDA+DMSVVAYLAG++DSYFF+NAP+ AFDILF NT
Sbjct: 61  SLSNSDRFHLIDTGNEPGDTITLAVDAQDMSVVAYLAGNDDSYFFSNAPKFAFDILFPNT 120

Query: 121 HKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQMIFE 180
           ++ LL+F+++F+SIEKAA TTRQATPLG + +N+AI+NLFHY   LAPVSFL+VFQMIFE
Sbjct: 121 NQNLLNFDSSFKSIEKAANTTRQATPLGFKESNAAIANLFHYDSVLAPVSFLIVFQMIFE 180

Query: 181 AAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIMLYNS 240
           +AKF F+EQ IVNSIK GE+FTP+LA++SLEDNWSELSLQIQAS SLQGLFGSS+ LYNS
Sbjct: 181 SAKFNFIEQRIVNSIKYGEAFTPDLAMLSLEDNWSELSLQIQASNSLQGLFGSSVTLYNS 240

Query: 241 RNEVIEVDSIYYPIILPNLALQLYHCNTQDYIKMP-GVVVQPENSRCYVKERTTRISGRD 300
           +NE IEVDSIYYP+IL NLALQLYHCN QDYIKMP   V   +N RCYVKERT RISGRD
Sbjct: 241 KNEAIEVDSIYYPLILSNLALQLYHCNIQDYIKMPTATVTDHQNPRCYVKERTVRISGRD 300

Query: 301 GFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPLAVIY 360
           G C DVA     DGS+VI  PC QQ NQ+WTF+ DHTIRS  KCLI  KS + NP AVI 
Sbjct: 301 GLCVDVA----RDGSQVISSPCGQQANQRWTFNRDHTIRSSDKCLIPTKSKA-NPFAVIL 360

Query: 361 NCSKISQQYATWDVSISGTIMNSANDLVLTSNKGSNDN-LIMENNIYGGSQGWRVGNYVD 420
           +C+K+SQQ ATWDVSISGTIMN A DLVLTSN G NDN L M+ N Y G+QGWRVGN+V+
Sbjct: 361 DCNKVSQQDATWDVSISGTIMNPAYDLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVE 420

Query: 421 PIITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVTASSI 480
           P+I SIIGV QMCLEA +ENTNIWLEECVKNK EQSWA+YSDGSIRV+N+ SLCVTASSI
Sbjct: 421 PLIVSIIGVKQMCLEATEENTNIWLEECVKNKIEQSWAVYSDGSIRVNNDHSLCVTASSI 480

Query: 481 DSYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHGLVNQ 540
           +  + IVI  CNGLA+QRWV   DGTIS PKNE LVMD+A++NVDLK I+LYPR  LV+Q
Sbjct: 481 EPKQRIVIGKCNGLASQRWVLNADGTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLVSQ 534

Query: 541 QWVVFY 545
           QWV  Y
Sbjct: 541 QWVALY 534

[Close]

BLAST of Lsi08G003420 vs. NCBI nr
Match: U3KRF8.2 (RecName: Full=Seed lectin; Short=SGSL; Contains: RecName: Full=Seed lectin Aalpha chain; Contains: RecName: Full=Seed lectin Abeta chain; Contains: RecName: Full=Seed lectin B chain [Trichosanthes anguina])

HSP 1 Score: 626.7 bits (1615), Expect = 1.9e-175
Identity = 322/529 (60.87%), Postives = 405/529 (76.56%), Query Frame = 0


Query: 21  ANLHLSKRPVIDVYKSFIQTVRKKFGSKTHNLYGIPILQHSLSNSDRFYLIDTGNVAGDT 80
           ANL LS+      YK+FI  VR++ GS+T+ LYGIP+L+HSLSNS+RFYL+   +   ++
Sbjct: 4   ANLRLSEAN-SGTYKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQDES 63

Query: 81  ITLAVDAEDMSVVAYL-AGDNDSYFFANAPQIAFDILFSNTHKTLLSFENTFQSIEKAAR 140
           ITLA+D EDM  VAY  AG ++SYFF NAPQIAF  LF++TH+ +L+F+NTF+S+E AA 
Sbjct: 64  ITLAIDVEDMVAVAYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENAAG 123

Query: 141 TTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQMIFEAAKFKFVEQAIVNSIKNGE 200
           TTRQ   LG++  + AISNLF+  P L P+SFLV+ QM+ EA+KF+F+EQ++  S KN +
Sbjct: 124 TTRQTIVLGVDPLDFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKNEK 183

Query: 201 SFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIMLYNSRNEVIEVDSIYYPIILPNL 260
           +F P+LAIVSLEDNWSE+SLQIQASTSLQGLFGS + LYNS NE+IEVDSIYYPIIL N+
Sbjct: 184 TFLPDLAIVSLEDNWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILANV 243

Query: 261 ALQLYHCNTQDYIKMPGVVVQPENSRCYVKERTTRISGRDGFCADVANAYFNDGSRVILF 320
           ALQLYHC            V   ++ C V+ RTTRISGRD  C DVA A  +DGSR+IL+
Sbjct: 244 ALQLYHCQ-----------VSTGDNECLVETRTTRISGRDALCVDVAGALTSDGSRLILY 303

Query: 321 PCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPLAVIYNCSKISQQYATWDVSISGTI 380
           PC QQVNQ+WTFHSD T+RSLGKCL +N S   N L VIY+CSK++ +  +WDVS+ GTI
Sbjct: 304 PCGQQVNQKWTFHSDGTVRSLGKCLATNNSKFGN-LVVIYDCSKLAAEDISWDVSVGGTI 363

Query: 381 MN-SANDLVLTSNKGS-NDNLIMENNIYGGSQGWRVGNYVDPIITSIIGVNQMCLEAKDE 440
           MN +  DL LTSNK + + NL ME N Y  SQGWRVGNYV PII SI+G++ MCLEA D 
Sbjct: 364 MNPNYEDLALTSNKATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATDG 423

Query: 441 NTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVTASS--IDSYKLIVILNCNGLATQ 500
           NTN+WLEECV N+REQSWA+YSDG+IRV +N+ LCVTASS   D++K+I ILNC+G   Q
Sbjct: 424 NTNMWLEECVPNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSNNQ 483

Query: 501 RWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHGLVNQQWVVFY 545
           RWVF  DG+IS P N+ L MD+A ++VDLK+IIL+  HG +NQQWV+FY
Sbjct: 484 RWVFLADGSISTPGNQRLAMDVARSDVDLKKIILHRPHGDLNQQWVLFY 519

[Close]

BLAST of Lsi08G003420 vs. NCBI nr
Match: XP_022156703.1 (seed lectin-like [Momordica charantia] >BAH05018.1 galactose-binding type-2 ribosome-inactivating protein [Momordica charantia])

HSP 1 Score: 515.8 bits (1327), Expect = 4.6e-142
Identity = 284/551 (51.54%), Postives = 377/551 (68.42%), Query Frame = 0


Query: 1   MRIIVAFI-VALCLATDGILAANLHLSKRPV-IDVYKSFIQTVRKKFG-SKTHNLYGIPI 60
           MR++  +I VAL L  +GI   NL LS+     D YKSFI+ +RK+     ++   GIPI
Sbjct: 3   MRVLAVYIVVALSLTINGI-ECNLSLSQSNFSADTYKSFIKNLRKQLTIGASYGSAGIPI 62

Query: 61  LQHSLSNSDRFYLIDTGNVAGDTITLAVDAEDMSVVAYLAGDNDSYFFANAPQIAFDILF 120
           L+HS+   +RF L+D  N   +TITLA++ ED    AY A D  SYFF NAP IA  ++F
Sbjct: 63  LKHSVPICERFLLVDLTNGDNETITLAINVEDAGFAAYRAADR-SYFFQNAPPIASYVIF 122

Query: 121 SNTHKTLLSFENTFQSIEKAARTTRQATPLGLESTNSAISNLFHYRPHLAPVSFLVVFQM 180
           ++T++ +++F NTF+SIE    TTR  TPLG+    ++I +LF +  +  P SFLV+ QM
Sbjct: 123 TDTNQNIMNFNNTFESIEIVGGTTRSETPLGIMHFEASIFHLFVHDENYVPTSFLVLIQM 182

Query: 181 IFEAAKFKFVEQAIVNSIKNGESFTPNLAIVSLEDNWSELSLQIQASTSLQGLFGSSIML 240
           + EAAKFKF+EQ +++SI + E FTP LA++SLE+NW++LSLQ+QAS SL G+FG S+ L
Sbjct: 183 VLEAAKFKFIEQKVIHSIMDMEDFTPGLAMLSLEENWTQLSLQLQASESLNGVFGDSVSL 242

Query: 241 YNSRNEVIEVDSIYYPIILPNLALQLYHCNTQDYIKMPGVVVQP--ENSRCYVKERTTRI 300
           YNS +E I VDS+YYPI+  N+A QLY C     I+M   +  P   N +C  ++RTTRI
Sbjct: 243 YNSMDEPIGVDSMYYPILTANMAFQLYQC-PYGVIRMMPTITMPNQNNEQCSPQQRTTRI 302

Query: 301 SGRDGFCADVANAYFNDGSRVILFPCRQQVNQQWTFHSDHTIRSLGKCLISNKSSSENPL 360
           SGRDG C DV  A   DGSRVIL+PC QQ NQQWTF+ D+TIRSLGKCL ++  SS + +
Sbjct: 303 SGRDGLCVDVYGALTADGSRVILYPCGQQQNQQWTFYPDNTIRSLGKCLATSALSSGSNV 362

Query: 361 AVIYNCSKISQQYATWDVSISGTIMNSANDLVLTSNKG-SNDNLIMENNIYGGSQGWRVG 420
            VI NC  +      W VS SGT+MN ++ LVLT+N   S  NL  ENN++   Q WR+G
Sbjct: 363 -VITNCDYLRYDDG-WMVSSSGTMMNKSSHLVLTANAATSRTNLTGENNVFAAKQAWRIG 422

Query: 421 NYVDPIITSIIGVNQMCLEAKDENTNIWLEECVKNKREQSWAIYSDGSIRVSNNQSLCVT 480
           NYV+PI+T+IIG+  MCLEA D +TN+WLE CVKNK +Q WA+YSD +IRV+NN++LCV+
Sbjct: 423 NYVEPIVTTIIGLRHMCLEATDNDTNVWLESCVKNKTKQYWALYSDDTIRVNNNRNLCVS 482

Query: 481 ASSIDSYKLIVILNCNGLATQRWVFKTDGTISPPKNENLVMDIAENNVDLKRIILYPRHG 540
           +S+  S KLIVI  C+G   QRWVF   GTIS P  E  VMD+A+N+V LK+I+L     
Sbjct: 483 SSTDSSSKLIVIRRCDGSINQRWVFTPQGTISNPGYE-AVMDVAQNDVYLKKIVLSSATD 542

Query: 541 LVN-QQWVVFY 545
             N QQW VFY
Sbjct: 543 KGNGQQWTVFY 547

The following BLAST results are available for this feature:

BLAST of Lsi08G003420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
U3KRF8	2.4e-178	60.87	Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2	[more]
P33183	6.4e-102	37.70	Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2	[more]
O22415	9.2e-101	39.37	Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=...	[more]
Q41358	6.6e-99	38.68	Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1	[more]
P93543	1.9e-93	38.14	Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1	[more]

BLAST of Lsi08G003420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KN71	3.4e-223	72.34	rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1	[more]
A0A5A7V3Q8	1.9e-221	72.16	rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G0...	[more]
A0A1S3CIM5	1.9e-221	72.16	rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103501401 PE=3 SV=1	[more]
B7X8M2	2.2e-142	51.54	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=MCL1 PE=1 SV=1	[more]
A0A0A0K979	1.5e-138	50.55	rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1	[more]

BLAST of Lsi08G003420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884532.1	1.4e-234	77.43	seed lectin-like [Benincasa hispida]	[more]
XP_004140357.1	7.0e-223	72.34	seed lectin [Cucumis sativus] >KGN51070.1 hypothetical protein Csa_008446 [Cucum...	[more]
XP_008463192.1	3.8e-221	72.16	PREDICTED: nigrin b-like [Cucumis melo] >KAA0062872.1 nigrin b-like [Cucumis mel...	[more]
U3KRF8.2	1.9e-175	60.87	RecName: Full=Seed lectin; Short=SGSL; Contains: RecName: Full=Seed lectin Aalph...	[more]
XP_022156703.1	4.6e-142	51.54	seed lectin-like [Momordica charantia] >BAH05018.1 galactose-binding type-2 ribo...	[more]

BLAST of Lsi08G003420 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 171..191 score: 43.98 coord: 205..222 score: 45.03 coord: 80..95 score: 41.61 coord: 32..45 score: 29.7 coord: 142..156 score: 37.89
IPR000772	Ricin B, lectin domain	SMART	SM00458	ricin_3	coord: 418..543 e-value: 8.8E-24 score: 95.0 coord: 290..414 e-value: 8.0E-18 score: 75.2
IPR000772	Ricin B, lectin domain	PFAM	PF00652	Ricin_B_lectin	coord: 424..540 e-value: 7.3E-20 score: 71.5 coord: 292..411 e-value: 6.2E-17 score: 62.0
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 289..414 score: 25.08
IPR000772	Ricin B, lectin domain	PROSITE	PS50231	RICIN_B_LECTIN	coord: 417..543 score: 33.571499
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 424..540 e-value: 3.94136E-21 score: 87.1758
IPR000772	Ricin B, lectin domain	CDD	cd00161	RICIN	coord: 294..411 e-value: 2.6868E-16 score: 73.3086
None	No IPR available	GENE3D	6.10.250.2640		coord: 21..62 e-value: 3.0E-14 score: 54.4
None	No IPR available	GENE3D	2.80.10.50		coord: 419..544 e-value: 2.9E-42 score: 145.3
None	No IPR available	GENE3D	2.80.10.50		coord: 284..418 e-value: 1.7E-27 score: 98.3
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 25..362
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 367..542
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 185..270 e-value: 4.4E-24 score: 86.2
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 32..221 e-value: 2.0E-26 score: 92.9
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 367..542 coord: 25..362
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 66..184 e-value: 2.0E-24 score: 88.0
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 420..541
IPR035992	Ricin B-like lectins	SUPERFAMILY	50370	Ricin B-like lectins	coord: 286..414
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 33..270

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G003420.1	Lsi08G003420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity