Lsi07G008320 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G008320
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionserine/threonine-protein kinase WAG1
Locationchr07: 9297927 .. 9299348 (+)
RNA-Seq ExpressionLsi07G008320
SyntenyLsi07G008320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGACGATTACTCCATCTTCCCTCCCGATTCAGATCTCGATTTCAGCTTCACTTCCACCGCCACCGACCGCACCTTCACCACCTCCTCCAGCGCCCGCACCAGTCTTGCTCGGAGCAGTCTAACACTCAGCTTCAATGAATCTCGTCTCTCTGCCGCTGCAGCCGCTGCTGCTGTCGTTAATCTCCGCCCCCACCGCCATTCTGACCCTCACTGGTCCGCCATTAAAGCCGCTACTACTCTCTCTTCCGACGGCCATCTTCACCTCCGTCACCTGAAACTCATCCGCCATCTCGGCACCGGCAACCTCGGCCGTGTGTTCTTGTGTCATTTGAGAGACAACGATCACGCCAGTTTCGCGCTGAAAGTTGTGGATAAGGAAGCTCTCAGTAACAAGAAGCTTTTGCAGGTCCAAACGGAAGCCGAGATTCTCGCTTCTTTGGACCACCCTTTTCTCCCAACTCTCTACGCGCGTCTTGACGTCTCTCACTACACGTGCCTCCTTATCGACTATTGTCCTGCCGGTGACCTCCACTCACTCCTCCGCAAACAGCCTGGTAACCGCCTTTCTGTCGCGGCCGCGAGATTCTTCGCCGCCGAGGTCCTGGTGGCGCTCGAGTATCTTCACGCGCTTGGGATCGTGTATCGCGATCTCAAGCCTGAGAACGTTCTTTTGCGTGAGGATGGCCACGTCATGCTCTCGGATTTCGACTTGTGTTTTAAGTCTGACGTGCTCCCTACTTTTCACACGTGGACTCGCCCGGGACCACAGGCTACTGGCTGCTGCTTCGGGTGGAGGACGGCGGAGGAGGAAGACGAGATTGTCGGGGAGTTTGTGGCGGAGCCGACGGCGGCGTTTTCGAAGTCGTGCGTGGGGACTCATGAGTACTTGGCGCCGGAGTTAGTGGCCGGTGGTGGCCACGGCAACGGCGTCGACTGGTGGGCTTTTGGGGTGTTCGTTTACGAGCTTCTGCACGGGACCACGCCGTTTAAAGGAGTAAATAAGGAGGGTACATTAAGGAACATTGCGATGTCGGAGGGGGTGAAGTTCCGGCCGGCGGCAGAGGAAGAGGAGGGGATGGCGGAGGCGAGAGATCTGATAGAGAGGCTGCTAGTGAAAGATCCGGCGAGGCGGTTGGGTTCCGGCAAAGGGGCGACGGAGATCAAACGGCATCCGTTTTTCGACGGGATAAAATGGCCGTTGATCAGGACATATAGGGCGCCTGAGGTGTATGGGCTGATGAGAAAAGCCAGATCCCACGTGAGCCACGTGACAGGCGTGACCCACCGCATGCGGCGGCGGGGCTGGTGGAACTGGAGGAAGCTCATCAGCAACCGTTTAATTAAAAATGGAAGTTCCAGTAAATCTCATACCAATACCAATTATTATAGTTATAGCTCCAAAAATAAGACGGGTTGA

mRNA sequence

ATGGACGACGATTACTCCATCTTCCCTCCCGATTCAGATCTCGATTTCAGCTTCACTTCCACCGCCACCGACCGCACCTTCACCACCTCCTCCAGCGCCCGCACCAGTCTTGCTCGGAGCAGTCTAACACTCAGCTTCAATGAATCTCGTCTCTCTGCCGCTGCAGCCGCTGCTGCTGTCGTTAATCTCCGCCCCCACCGCCATTCTGACCCTCACTGGTCCGCCATTAAAGCCGCTACTACTCTCTCTTCCGACGGCCATCTTCACCTCCGTCACCTGAAACTCATCCGCCATCTCGGCACCGGCAACCTCGGCCGTGTGTTCTTGTGTCATTTGAGAGACAACGATCACGCCAGTTTCGCGCTGAAAGTTGTGGATAAGGAAGCTCTCAGTAACAAGAAGCTTTTGCAGGTCCAAACGGAAGCCGAGATTCTCGCTTCTTTGGACCACCCTTTTCTCCCAACTCTCTACGCGCGTCTTGACGTCTCTCACTACACGTGCCTCCTTATCGACTATTGTCCTGCCGGTGACCTCCACTCACTCCTCCGCAAACAGCCTGGTAACCGCCTTTCTGTCGCGGCCGCGAGATTCTTCGCCGCCGAGGTCCTGGTGGCGCTCGAGTATCTTCACGCGCTTGGGATCGTGTATCGCGATCTCAAGCCTGAGAACGTTCTTTTGCGTGAGGATGGCCACGTCATGCTCTCGGATTTCGACTTGTGTTTTAAGTCTGACGTGCTCCCTACTTTTCACACGTGGACTCGCCCGGGACCACAGGCTACTGGCTGCTGCTTCGGGTGGAGGACGGCGGAGGAGGAAGACGAGATTGTCGGGGAGTTTGTGGCGGAGCCGACGGCGGCGTTTTCGAAGTCGTGCGTGGGGACTCATGAGTACTTGGCGCCGGAGTTAGTGGCCGGTGGTGGCCACGGCAACGGCGTCGACTGGTGGGCTTTTGGGGTGTTCGTTTACGAGCTTCTGCACGGGACCACGCCGTTTAAAGGAGTAAATAAGGAGGGTACATTAAGGAACATTGCGATGTCGGAGGGGGTGAAGTTCCGGCCGGCGGCAGAGGAAGAGGAGGGGATGGCGGAGGCGAGAGATCTGATAGAGAGGCTGCTAGTGAAAGATCCGGCGAGGCGGTTGGGTTCCGGCAAAGGGGCGACGGAGATCAAACGGCATCCGTTTTTCGACGGGATAAAATGGCCGTTGATCAGGACATATAGGGCGCCTGAGGTGTATGGGCTGATGAGAAAAGCCAGATCCCACGTGAGCCACGTGACAGGCGTGACCCACCGCATGCGGCGGCGGGGCTGGTGGAACTGGAGGAAGCTCATCAGCAACCGTTTAATTAAAAATGGAAGTTCCAGTAAATCTCATACCAATACCAATTATTATAGTTATAGCTCCAAAAATAAGACGGGTTGA

Coding sequence (CDS)

ATGGACGACGATTACTCCATCTTCCCTCCCGATTCAGATCTCGATTTCAGCTTCACTTCCACCGCCACCGACCGCACCTTCACCACCTCCTCCAGCGCCCGCACCAGTCTTGCTCGGAGCAGTCTAACACTCAGCTTCAATGAATCTCGTCTCTCTGCCGCTGCAGCCGCTGCTGCTGTCGTTAATCTCCGCCCCCACCGCCATTCTGACCCTCACTGGTCCGCCATTAAAGCCGCTACTACTCTCTCTTCCGACGGCCATCTTCACCTCCGTCACCTGAAACTCATCCGCCATCTCGGCACCGGCAACCTCGGCCGTGTGTTCTTGTGTCATTTGAGAGACAACGATCACGCCAGTTTCGCGCTGAAAGTTGTGGATAAGGAAGCTCTCAGTAACAAGAAGCTTTTGCAGGTCCAAACGGAAGCCGAGATTCTCGCTTCTTTGGACCACCCTTTTCTCCCAACTCTCTACGCGCGTCTTGACGTCTCTCACTACACGTGCCTCCTTATCGACTATTGTCCTGCCGGTGACCTCCACTCACTCCTCCGCAAACAGCCTGGTAACCGCCTTTCTGTCGCGGCCGCGAGATTCTTCGCCGCCGAGGTCCTGGTGGCGCTCGAGTATCTTCACGCGCTTGGGATCGTGTATCGCGATCTCAAGCCTGAGAACGTTCTTTTGCGTGAGGATGGCCACGTCATGCTCTCGGATTTCGACTTGTGTTTTAAGTCTGACGTGCTCCCTACTTTTCACACGTGGACTCGCCCGGGACCACAGGCTACTGGCTGCTGCTTCGGGTGGAGGACGGCGGAGGAGGAAGACGAGATTGTCGGGGAGTTTGTGGCGGAGCCGACGGCGGCGTTTTCGAAGTCGTGCGTGGGGACTCATGAGTACTTGGCGCCGGAGTTAGTGGCCGGTGGTGGCCACGGCAACGGCGTCGACTGGTGGGCTTTTGGGGTGTTCGTTTACGAGCTTCTGCACGGGACCACGCCGTTTAAAGGAGTAAATAAGGAGGGTACATTAAGGAACATTGCGATGTCGGAGGGGGTGAAGTTCCGGCCGGCGGCAGAGGAAGAGGAGGGGATGGCGGAGGCGAGAGATCTGATAGAGAGGCTGCTAGTGAAAGATCCGGCGAGGCGGTTGGGTTCCGGCAAAGGGGCGACGGAGATCAAACGGCATCCGTTTTTCGACGGGATAAAATGGCCGTTGATCAGGACATATAGGGCGCCTGAGGTGTATGGGCTGATGAGAAAAGCCAGATCCCACGTGAGCCACGTGACAGGCGTGACCCACCGCATGCGGCGGCGGGGCTGGTGGAACTGGAGGAAGCTCATCAGCAACCGTTTAATTAAAAATGGAAGTTCCAGTAAATCTCATACCAATACCAATTATTATAGTTATAGCTCCAAAAATAAGACGGGTTGA

Protein sequence

MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAVVNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVLPTFHTWTRPGPQATGCCFGWRTAEEEDEIVGEFVAEPTAAFSKSCVGTHEYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEEGMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKARSHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSSKNKTG
Homology
BLAST of Lsi07G008320 vs. ExPASy Swiss-Prot
Match: Q9C8M5 (Serine/threonine-protein kinase WAG1 OS=Arabidopsis thaliana OX=3702 GN=WAG1 PE=2 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 3.1e-169
Identity = 313/481 (65.07%), Postives = 367/481 (76.30%), Query Frame = 0

Query: 2   DDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAA----AAA 61
           DD Y +   D+DLD SFTSTATDRTF TSSSAR+SLARSSLTLSFN+ RLS A       
Sbjct: 3   DDGYYL---DTDLDLSFTSTATDRTF-TSSSARSSLARSSLTLSFND-RLSTATTPSTTT 62

Query: 62  AAVVNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRD-ND 121
           ++      HR  DPHW++I+AATTLSSDG LHLRH KL+RHLGTGNLGRVFLCHLRD  +
Sbjct: 63  SSAATTLHHRRYDPHWTSIRAATTLSSDGRLHLRHFKLVRHLGTGNLGRVFLCHLRDCPN 122

Query: 122 HASFALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAG 181
              FALKV+D++ L+ KK+  V+TEAEIL+ LDHPFLPTLYAR+D SHYTCLLIDYCP G
Sbjct: 123 PTGFALKVIDRDVLTAKKISHVETEAEILSLLDHPFLPTLYARIDASHYTCLLIDYCPNG 182

Query: 182 DLHSLLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSD 241
           DLHSLLRKQP NRL ++  RFFAAEVLVALEYLHALGIVYRDLKPEN+L+REDGH+MLSD
Sbjct: 183 DLHSLLRKQPNNRLPISPVRFFAAEVLVALEYLHALGIVYRDLKPENILIREDGHIMLSD 242

Query: 242 FDLCFKSDVLPTFHT----WTRPGPQAT---GCCFGWRTAEEEDEIVGEFVAEPTAAFSK 301
           FDLCFK+DV+PTF +     T   P+ T   G CF      E +EIV EF AEP  AFSK
Sbjct: 243 FDLCFKADVVPTFRSRRFRRTSSSPRKTRRGGGCFSTEVEYEREEIVAEFAAEPVTAFSK 302

Query: 302 SCVGTHEYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGV 361
           SCVGTHEYLAPELVAG GHG+GVDWWAFG+F+YE+L+GTTPFKG  KE TLRNI  ++ V
Sbjct: 303 SCVGTHEYLAPELVAGNGHGSGVDWWAFGIFLYEMLYGTTPFKGGTKEQTLRNIVSNDDV 362

Query: 362 KFRPAAEEEEGMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAP 421
            F     EEEGM EA+DLIE+LLVKDP +RLG  +GA +IKRH FF+GIKWPLIR Y+ P
Sbjct: 363 AF---TLEEEGMVEAKDLIEKLLVKDPRKRLGCARGAQDIKRHEFFEGIKWPLIRNYKPP 422

Query: 422 EVYGLMRKARSHVSHVT-GVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSS 470
           E+ GL++K ++H  HVT  VT R  +  WW    L+ ++ +   SSSK  +N NYY Y  
Sbjct: 423 EIRGLVKKTKAHAGHVTAAVTPRRNKWLWWALSHLLRSKSLSK-SSSKIQSNNNYYHYVG 474

BLAST of Lsi07G008320 vs. ExPASy Swiss-Prot
Match: Q9LUL2 (Serine/threonine-protein kinase WAG2 OS=Arabidopsis thaliana OX=3702 GN=WAG2 PE=2 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.1e-154
Identity = 305/480 (63.54%), Postives = 359/480 (74.79%), Query Frame = 0

Query: 10  PDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNE--SRLSAAAAAAAVVNLRPHR 69
           PD+DLD SFTST TDRTF +SS      AR+SLTLSFN+  S  SA   ++   +   HR
Sbjct: 9   PDTDLDLSFTSTTTDRTFASSS------ARTSLTLSFNDRLSTSSAVTTSSTSSSSVNHR 68

Query: 70  HSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDK 129
             DPHWSAIK+A  LSSDG++HLRHLKLIRHLGTGNLGRVFLC+LRD+  A FALKV+D+
Sbjct: 69  RHDPHWSAIKSAKLLSSDGNIHLRHLKLIRHLGTGNLGRVFLCNLRDSS-ARFALKVIDR 128

Query: 130 EAL-SNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQP 189
             L + KKL QV+TEAEIL+ LDHPFLPTLYAR+D SHYTCLLIDY P GDLHSLLRKQP
Sbjct: 129 NCLTTEKKLSQVETEAEILSLLDHPFLPTLYARIDESHYTCLLIDYAPNGDLHSLLRKQP 188

Query: 190 GNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVL 249
           GNRL +   RFFAAEVLVALEYLHA+GIVYRDLKPENVLLREDGHVMLSDFDLCFKSDV+
Sbjct: 189 GNRLPIQPVRFFAAEVLVALEYLHAMGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVV 248

Query: 250 PTFHT--WTRPGPQAT-----GCCFGWRTAE--EEDEIVGEFVAEPTAAFSKSCVGTHEY 309
           PTF +  + R     +       CF     +  E +EIV EF AEP  AFS+SCVGTHEY
Sbjct: 249 PTFKSRRYRRSSSSPSLRRRRSGCFSVAAEKKYEREEIVSEFAAEPVTAFSRSCVGTHEY 308

Query: 310 LAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEE 369
           LAPELV+G GHG+GVDWWAFG+F+YELL+GTTPFKG +KE TLRNI  +     + A+  
Sbjct: 309 LAPELVSGNGHGSGVDWWAFGIFLYELLYGTTPFKGESKEQTLRNIVSTT----KTASFH 368

Query: 370 EEG-MAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAP-EVYGLM 429
            +G + EARDLIE+LLVKDP +RLG  +GA +IKRHPFFDGIKWPLIR Y+ P EV GL+
Sbjct: 369 MDGDLDEARDLIEKLLVKDPRKRLGCARGAQDIKRHPFFDGIKWPLIRHYKPPEEVRGLV 428

Query: 430 --RKARSHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSS---SKSHTNTNYYSYSSKN 471
             +  R H SHV  V+   RRR  + WR L  + L++  SS   SK+ +N+NYY Y  K+
Sbjct: 429 IKKSTRPHASHVIAVS--PRRRKSFLWRAL--SYLLRGKSSSGGSKNQSNSNYYHYVGKS 473

BLAST of Lsi07G008320 vs. ExPASy Swiss-Prot
Match: Q2QM77 (Protein kinase PINOID OS=Oryza sativa subsp. japonica OX=39947 GN=PID PE=2 SV=1)

HSP 1 Score: 340.9 bits (873), Expect = 2.3e-92
Identity = 199/417 (47.72%), Postives = 265/417 (63.55%), Query Frame = 0

Query: 20  STATDRTFTTSSSARTSLAR-SSLT-LSFNESRLSAAAAAA-------AVVNLRPHRHSD 79
           +TA + + ++++S+  SLAR SSL+ LSF+ S  +A AAAA       A V LRPHR  D
Sbjct: 30  TTAPNSSLSSAASSTGSLARCSSLSRLSFDCSPSAAVAAAATSCSPPRASVLLRPHRSGD 89

Query: 80  PHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDH----ASFALKVVD 139
             W+AI+AA+T S+   L  R  KL+R +G G++G V+LC LR +        +A+KVVD
Sbjct: 90  VAWAAIRAASTTSA-APLGPRDFKLVRRIGGGDIGTVYLCRLRSSPERESPCMYAMKVVD 149

Query: 140 KEALSNK-KLLQVQTEAEILASLDHPFLPTLYARLDVS-HYTCLLIDYCPAGDLHSLLRK 199
           + A++ K KL +   E  IL  LDHPFLPTL+A  D + H++C ++++CP GDLHSL  +
Sbjct: 150 RRAVARKQKLGRAAAEKRILRQLDHPFLPTLFADFDATPHFSCAVMEFCPGGDLHSLRHR 209

Query: 200 QPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSD 259
            P  R  + +ARF+AAEVL+A+EYLH +GIVYRDLKPENVL+R DGH+ML+DFDL  +S 
Sbjct: 210 MPSRRFPLPSARFYAAEVLLAIEYLHMMGIVYRDLKPENVLIRADGHIMLTDFDLSLQST 269

Query: 260 VLPTFHTWTRPGPQATG--CCFG---WRTAEEEDEIVG---EFVAEPTAAFSKSCVGTHE 319
             P+    T    +A+G   CF     R     + +      FVAEP  A S S VGTHE
Sbjct: 270 TSPSLDGDTDTDDEASGGASCFPDHLLRFKRRRNAVAAPRPRFVAEPVDARSCSFVGTHE 329

Query: 320 YLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAE 379
           Y+APE+ +GG HG  VDWWA+GVF+YEL++G TPF G   E TLRNI          +  
Sbjct: 330 YVAPEVASGGAHGAAVDWWAYGVFLYELIYGRTPFAGATNEATLRNIVRRPLAFPSGSGS 389

Query: 380 EEEGMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYG 414
                A+ARDLI RLL KDPA RLGS +GA ++K HPFF  +   L+R+ R P V G
Sbjct: 390 CGPADADARDLIARLLAKDPAARLGSRRGAADVKSHPFFKSLNLALLRSSRPPVVPG 445

BLAST of Lsi07G008320 vs. ExPASy Swiss-Prot
Match: Q94E49 (Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 2.2e-90
Identity = 193/451 (42.79%), Postives = 261/451 (57.87%), Query Frame = 0

Query: 23  TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAVVNLRPHRHSDPHWSAIKAATTL 82
           + R+  T+  +R S      + S   +R       A+    +PH+ +   W AI+     
Sbjct: 12  SSRSSLTAPDSRRSWISDIGSSSSVSARSFGGDTPASSCRYKPHKANQAEWEAIRRLR-- 71

Query: 83  SSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHAS----------FALKVVDKEALS- 142
           +  G + L H +L+R LG+G+LG V+LC LR+   +S          +A+KVVDK+AL+ 
Sbjct: 72  AGAGRVGLEHFRLVRRLGSGDLGNVYLCRLREPWSSSSMTTTAGGCLYAMKVVDKDALAF 131

Query: 143 NKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLS 202
            KKL + + E +IL +LDHPFLPTLYA  + SHY CL++++CP GDLH   ++QPG R +
Sbjct: 132 RKKLRRAEVERDILRTLDHPFLPTLYADFEASHYACLVMEFCPGGDLHVARQRQPGRRFT 191

Query: 203 VAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVLPTFHT 262
           V++ RF+ AE ++ALEYLH +G+VYRDLKPENVL+R DGH+MLSDFDL  K DV+P    
Sbjct: 192 VSSTRFYVAETVLALEYLHMMGVVYRDLKPENVLVRGDGHIMLSDFDLSLKCDVVPKLLR 251

Query: 263 WTR--------PGPQATGC------------------------CFGWRTA---------- 322
             R        P P  + C                        C G   A          
Sbjct: 252 PARSAAAGGKPPLPPPSSCVPPTIQPVLSCIFRGVHKCHHAKECAGGGAAAGNNGDGDGN 311

Query: 323 -EEEDEIVGE---FVAEPTAAFSKSCVGTHEYLAPELVAGGGHGNGVDWWAFGVFVYELL 382
            EE +    E    V EP AA SKS VGTHEYLAPE+++G GHG+ VDWW  GVF+YE+L
Sbjct: 312 DEEAETETAEPEVVVVEPVAARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGVFMYEML 371

Query: 383 HGTTPFKGVNKEGTLRNIAMSEGVKF-----RPAAEEEEGMAEARDLIERLLVKDPARRL 412
           +G TPFKG + E TL NI + + V F       AA E E M  A+DL+ +LL K+P +RL
Sbjct: 372 YGRTPFKGESNEKTLINI-IKQPVTFPRLAGAAAAGEWEEMKTAQDLMLQLLAKNPKKRL 431

BLAST of Lsi07G008320 vs. ExPASy Swiss-Prot
Match: P15792 (Protein kinase PVPK-1 OS=Phaseolus vulgaris OX=3885 PE=2 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 2.4e-89
Identity = 197/453 (43.49%), Postives = 258/453 (56.95%), Query Frame = 0

Query: 12  SDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAA------------- 71
           S+   SFT+T      +   S  + +A SS    FNESR ++   A+             
Sbjct: 139 SEAKESFTNTEVSECASVDKSCESEVANSS---DFNESRKTSICRASTGSDASDESSTSS 198

Query: 72  -AVVNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDH 131
            + V  +PH+ +D  W AI+A  T   DG L +RH +L++ LG G++G V+L  L     
Sbjct: 199 LSSVLYKPHKANDIRWEAIQAVRT--RDGMLEMRHFRLLKKLGCGDIGSVYLAEL-SGTR 258

Query: 132 ASFALKVVDKEALSN-KKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAG 191
            SFA+KV++K  L+N KKLL+ QTE EIL SLDHPFLPTLY   +   ++CL++++CP G
Sbjct: 259 TSFAMKVMNKTELANRKKLLRAQTEREILQSLDHPFLPTLYTHFETEIFSCLVMEFCPGG 318

Query: 192 DLHSLLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSD 251
           DLH+L ++QPG   S  A RF+ AEVL++LEYLH LGI+YRDLKPENVL+REDGH+MLSD
Sbjct: 319 DLHALRQRQPGKYFSEHAVRFYVAEVLLSLEYLHMLGIIYRDLKPENVLVREDGHIMLSD 378

Query: 252 FDLCFKSDVLPTF------------HTWTRPGPQATGC----------CFGWRTAEEEDE 311
           FDL  +  V PT                 +P      C          CF  R    + +
Sbjct: 379 FDLSLRCSVSPTLVKSSNNLQTKSSGYCVQPSCIEPTCVMQPDCIKPSCFTPRFLSGKSK 438

Query: 312 ----------------IVGEFVAEPTAAFSKSCVGTHEYLAPELVAGGGHGNGVDWWAFG 371
                            + E +AEPT A S S VGTHEYLAPE++ G GHG+ VDWW FG
Sbjct: 439 KDKKSKPKNDMHNQVTPLPELIAEPTNARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFG 498

Query: 372 VFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEEGMAEARDLIERLLVKDPAR 412
           +F+YELL G TPFKG     TL N+ + + ++F    E       ARDLI  LLVK+P  
Sbjct: 499 IFLYELLFGRTPFKGSANRATLFNV-IGQPLRF---PESPTVSFAARDLIRGLLVKEPQH 558

BLAST of Lsi07G008320 vs. ExPASy TrEMBL
Match: A0A5D3D1P2 (Serine/threonine-protein kinase WAG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G001730 PE=4 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 3.4e-248
Identity = 431/474 (90.93%), Postives = 448/474 (94.51%), Query Frame = 0

Query: 1   MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAV 60
           M+DDYSIFPPDSDLDFSFTST TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA   
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTTTDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA--- 60

Query: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120
           +NLRPH  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF
Sbjct: 61  LNLRPHHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120

Query: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180
           ALKVVDKEALSNKKL QVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS
Sbjct: 121 ALKVVDKEALSNKKLSQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180

Query: 181 LLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240
           LLRKQPG+R SVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC
Sbjct: 181 LLRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240

Query: 241 FKSDVLPTFHTWTRPGPQATGCCFGWRTA-EEEDEIVGEFVAEPTAAFSKSCVGTHEYLA 300
           FKSDV+PTFHTWTRPGP+ATGCC GWRT  ++E+EIVGEFVAEPTAAFSKSCVGTHEYLA
Sbjct: 241 FKSDVVPTFHTWTRPGPKATGCCLGWRTTPDDEEEIVGEFVAEPTAAFSKSCVGTHEYLA 300

Query: 301 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEE 360
           PELV+GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA SEGVKFR A EEEE
Sbjct: 301 PELVSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFRAAGEEEE 360

Query: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420
           GM EARDLIERLLVKDPA RLGS KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK R
Sbjct: 361 GMGEARDLIERLLVKDPAMRLGSEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKGR 420

Query: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSSKNKTG 474
           S VSHV GV+ R+RRRGWWNWRK+ISN LIKNGS SKSH+NTN+YSYSSK KTG
Sbjct: 421 SQVSHVRGVSQRIRRRGWWNWRKVISNGLIKNGSCSKSHSNTNFYSYSSKKKTG 471

BLAST of Lsi07G008320 vs. ExPASy TrEMBL
Match: A0A1S3BRT1 (serine/threonine-protein kinase WAG1 OS=Cucumis melo OX=3656 GN=LOC103492987 PE=4 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 3.4e-248
Identity = 431/474 (90.93%), Postives = 448/474 (94.51%), Query Frame = 0

Query: 1   MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAV 60
           M+DDYSIFPPDSDLDFSFTST TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA   
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTTTDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA--- 60

Query: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120
           +NLRPH  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF
Sbjct: 61  LNLRPHHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120

Query: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180
           ALKVVDKEALSNKKL QVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS
Sbjct: 121 ALKVVDKEALSNKKLSQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180

Query: 181 LLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240
           LLRKQPG+R SVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC
Sbjct: 181 LLRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240

Query: 241 FKSDVLPTFHTWTRPGPQATGCCFGWRTA-EEEDEIVGEFVAEPTAAFSKSCVGTHEYLA 300
           FKSDV+PTFHTWTRPGP+ATGCC GWRT  ++E+EIVGEFVAEPTAAFSKSCVGTHEYLA
Sbjct: 241 FKSDVVPTFHTWTRPGPKATGCCLGWRTTPDDEEEIVGEFVAEPTAAFSKSCVGTHEYLA 300

Query: 301 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEE 360
           PELV+GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA SEGVKFR A EEEE
Sbjct: 301 PELVSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFRAAGEEEE 360

Query: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420
           GM EARDLIERLLVKDPA RLGS KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK R
Sbjct: 361 GMGEARDLIERLLVKDPAMRLGSEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKGR 420

Query: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSSKNKTG 474
           S VSHV GV+ R+RRRGWWNWRK+ISN LIKNGS SKSH+NTN+YSYSSK KTG
Sbjct: 421 SQVSHVRGVSQRIRRRGWWNWRKVISNGLIKNGSCSKSHSNTNFYSYSSKKKTG 471

BLAST of Lsi07G008320 vs. ExPASy TrEMBL
Match: A0A5A7V4J3 (Serine/threonine-protein kinase WAG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold468G00580 PE=4 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 8.5e-247
Identity = 429/474 (90.51%), Postives = 447/474 (94.30%), Query Frame = 0

Query: 1   MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAV 60
           M+DDYSIFPPDSDLDFSFTST TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA   
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTTTDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA--- 60

Query: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120
           +NLRPH  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF
Sbjct: 61  LNLRPHHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120

Query: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180
           ALKVVDKEALSNKKL QVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS
Sbjct: 121 ALKVVDKEALSNKKLSQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180

Query: 181 LLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240
           LLRKQPG+R SVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC
Sbjct: 181 LLRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240

Query: 241 FKSDVLPTFHTWTRPGPQATGCCFGWRTA-EEEDEIVGEFVAEPTAAFSKSCVGTHEYLA 300
           FKSDV+PTFHTWTRPGP+ATGCC GWRT  ++E+EIVGEFVAEPTAAFSKSCVGTHEYLA
Sbjct: 241 FKSDVVPTFHTWTRPGPKATGCCLGWRTTPDDEEEIVGEFVAEPTAAFSKSCVGTHEYLA 300

Query: 301 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEE 360
           PEL++GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKE TLRNIA SEGVKFR A EEEE
Sbjct: 301 PELLSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEVTLRNIASSEGVKFRAAGEEEE 360

Query: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420
           GM EARDLIERLLVKDPA RLGS KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK R
Sbjct: 361 GMGEARDLIERLLVKDPAMRLGSEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKGR 420

Query: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSSKNKTG 474
           S VSHV GV+ R+RRRGWWNWRK+ISN LIKNGS SKSH+NTN+YSYSSK KTG
Sbjct: 421 SQVSHVRGVSQRIRRRGWWNWRKVISNGLIKNGSCSKSHSNTNFYSYSSKKKTG 471

BLAST of Lsi07G008320 vs. ExPASy TrEMBL
Match: Q9XGL2 (CsPK3 OS=Cucumis sativus OX=3659 GN=CsPK3 PE=2 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.0e-244
Identity = 427/474 (90.08%), Postives = 443/474 (93.46%), Query Frame = 0

Query: 1   MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAV 60
           M+DDYSIFPPDSDLDFSFTST TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA   
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTTTDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA--- 60

Query: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120
           +NLRP+  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF
Sbjct: 61  LNLRPYHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120

Query: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180
           ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS
Sbjct: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180

Query: 181 LLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240
           LLRKQPG+R SVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC
Sbjct: 181 LLRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240

Query: 241 FKSDVLPTFHTWTRPGPQATGCCFGWRTAEE-EDEIVGEFVAEPTAAFSKSCVGTHEYLA 300
           FKSDV+PTFHTWTRPGPQATG CFGWRT+ E E+EIVGEFVAEPT+AFSKSCVGTHEYLA
Sbjct: 241 FKSDVVPTFHTWTRPGPQATGSCFGWRTSPEFEEEIVGEFVAEPTSAFSKSCVGTHEYLA 300

Query: 301 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEE 360
           PELV GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA SEGVKFR   EEE 
Sbjct: 301 PELVTGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFRGGGEEEG 360

Query: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420
           GM EARDLIERLLVKDP RRLG  KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK R
Sbjct: 361 GMGEARDLIERLLVKDPGRRLGWEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKGR 420

Query: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSSKNKTG 474
           + VSHV  VT R+RRRGWWNWRK++  RLIKNGS SKSH+NTNYYSYSSK KTG
Sbjct: 421 AQVSHVREVTERIRRRGWWNWRKVMRKRLIKNGSCSKSHSNTNYYSYSSKKKTG 471

BLAST of Lsi07G008320 vs. ExPASy TrEMBL
Match: A0A6J1J9F8 (serine/threonine-protein kinase WAG1-like OS=Cucurbita maxima OX=3661 GN=LOC111482931 PE=4 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.4e-241
Identity = 426/466 (91.42%), Postives = 439/466 (94.21%), Query Frame = 0

Query: 1   MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAV 60
           M+DDYS FPPDSDLDFSFTST TDRTF  SSSARTSLARSSLTLSFNESRL+AAAAAAA 
Sbjct: 1   MEDDYSFFPPDSDLDFSFTSTTTDRTF-ASSSARTSLARSSLTLSFNESRLAAAAAAAAA 60

Query: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120
           V+ RPH HSDPHWSAIKAAT LSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF
Sbjct: 61  VHHRPHHHSDPHWSAIKAATALSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120

Query: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180
           ALKVVDK+ALSNKKLLQVQTE+EILASL+HPFLPTLYARLDVSHYTCLLIDYCP+GDLHS
Sbjct: 121 ALKVVDKDALSNKKLLQVQTESEILASLNHPFLPTLYARLDVSHYTCLLIDYCPSGDLHS 180

Query: 181 LLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240
           LLRKQP  R SVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC
Sbjct: 181 LLRKQPNYRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240

Query: 241 FKSDVLPTFHTWTRPGPQATGCCFGWRTAE-EEDEIVGEFVAEPTAAFSKSCVGTHEYLA 300
           FKS+V+PTFHTWT+PGPQ+T  CFGWRTA  E  EIVGEFVAEPT AFSKSCVGTHEYLA
Sbjct: 241 FKSEVVPTFHTWTQPGPQSTS-CFGWRTAAVESQEIVGEFVAEPTTAFSKSCVGTHEYLA 300

Query: 301 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEE 360
           PELV GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA SEGVKFR AA EEE
Sbjct: 301 PELVTGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFR-AAVEEE 360

Query: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420
           GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR
Sbjct: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420

Query: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYS 466
           SHVSHVTG +HRMRRRGWWNWRKL SN L KNGS+SKSHTN NYYS
Sbjct: 421 SHVSHVTGASHRMRRRGWWNWRKL-SNGLFKNGSASKSHTNNNYYS 462

BLAST of Lsi07G008320 vs. NCBI nr
Match: XP_038899976.1 (serine/threonine-protein kinase WAG1-like [Benincasa hispida])

HSP 1 Score: 927.2 bits (2395), Expect = 5.8e-266
Identity = 459/474 (96.84%), Postives = 468/474 (98.73%), Query Frame = 0

Query: 1   MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAV 60
           M+ DYSIFPPDSDLDFSFTSTATDRTFTTSSSAR+SLARSSLTLSFNESRLSAAAAAAA+
Sbjct: 1   MEYDYSIFPPDSDLDFSFTSTATDRTFTTSSSARSSLARSSLTLSFNESRLSAAAAAAAI 60

Query: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120
           VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF
Sbjct: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120

Query: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180
           ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS
Sbjct: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180

Query: 181 LLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240
           LLRKQPGNR SVAAARFFAAEVLVALEYLH+LGIVYRDLKPENVLLREDGHVMLSDFDLC
Sbjct: 181 LLRKQPGNRFSVAAARFFAAEVLVALEYLHSLGIVYRDLKPENVLLREDGHVMLSDFDLC 240

Query: 241 FKSDVLPTFHTWTRPG-PQATGCCFGWRTAEEEDEIVGEFVAEPTAAFSKSCVGTHEYLA 300
           FKSDV+PTFHTWTRPG PQ TGCCFGWRTAEEE+EIVGEFVAEPTAAFSKSCVGTHEYLA
Sbjct: 241 FKSDVVPTFHTWTRPGPPQPTGCCFGWRTAEEEEEIVGEFVAEPTAAFSKSCVGTHEYLA 300

Query: 301 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEE 360
           PELV+GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA SEG+KFRPAAEEEE
Sbjct: 301 PELVSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGMKFRPAAEEEE 360

Query: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420
           GMAEARDLIERLLVKDPARR+GSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR
Sbjct: 361 GMAEARDLIERLLVKDPARRMGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420

Query: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSSKNKTG 474
           SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYS KNKTG
Sbjct: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSFKNKTG 474

BLAST of Lsi07G008320 vs. NCBI nr
Match: XP_008451816.1 (PREDICTED: serine/threonine-protein kinase WAG1 [Cucumis melo] >TYK16379.1 serine/threonine-protein kinase WAG1 [Cucumis melo var. makuwa])

HSP 1 Score: 867.1 bits (2239), Expect = 7.1e-248
Identity = 431/474 (90.93%), Postives = 448/474 (94.51%), Query Frame = 0

Query: 1   MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAV 60
           M+DDYSIFPPDSDLDFSFTST TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA   
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTTTDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA--- 60

Query: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120
           +NLRPH  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF
Sbjct: 61  LNLRPHHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120

Query: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180
           ALKVVDKEALSNKKL QVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS
Sbjct: 121 ALKVVDKEALSNKKLSQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180

Query: 181 LLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240
           LLRKQPG+R SVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC
Sbjct: 181 LLRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240

Query: 241 FKSDVLPTFHTWTRPGPQATGCCFGWRTA-EEEDEIVGEFVAEPTAAFSKSCVGTHEYLA 300
           FKSDV+PTFHTWTRPGP+ATGCC GWRT  ++E+EIVGEFVAEPTAAFSKSCVGTHEYLA
Sbjct: 241 FKSDVVPTFHTWTRPGPKATGCCLGWRTTPDDEEEIVGEFVAEPTAAFSKSCVGTHEYLA 300

Query: 301 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEE 360
           PELV+GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA SEGVKFR A EEEE
Sbjct: 301 PELVSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFRAAGEEEE 360

Query: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420
           GM EARDLIERLLVKDPA RLGS KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK R
Sbjct: 361 GMGEARDLIERLLVKDPAMRLGSEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKGR 420

Query: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSSKNKTG 474
           S VSHV GV+ R+RRRGWWNWRK+ISN LIKNGS SKSH+NTN+YSYSSK KTG
Sbjct: 421 SQVSHVRGVSQRIRRRGWWNWRKVISNGLIKNGSCSKSHSNTNFYSYSSKKKTG 471

BLAST of Lsi07G008320 vs. NCBI nr
Match: KAA0062963.1 (serine/threonine-protein kinase WAG1 [Cucumis melo var. makuwa])

HSP 1 Score: 862.4 bits (2227), Expect = 1.8e-246
Identity = 429/474 (90.51%), Postives = 447/474 (94.30%), Query Frame = 0

Query: 1   MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAV 60
           M+DDYSIFPPDSDLDFSFTST TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA   
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTTTDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA--- 60

Query: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120
           +NLRPH  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF
Sbjct: 61  LNLRPHHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120

Query: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180
           ALKVVDKEALSNKKL QVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS
Sbjct: 121 ALKVVDKEALSNKKLSQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180

Query: 181 LLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240
           LLRKQPG+R SVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC
Sbjct: 181 LLRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240

Query: 241 FKSDVLPTFHTWTRPGPQATGCCFGWRTA-EEEDEIVGEFVAEPTAAFSKSCVGTHEYLA 300
           FKSDV+PTFHTWTRPGP+ATGCC GWRT  ++E+EIVGEFVAEPTAAFSKSCVGTHEYLA
Sbjct: 241 FKSDVVPTFHTWTRPGPKATGCCLGWRTTPDDEEEIVGEFVAEPTAAFSKSCVGTHEYLA 300

Query: 301 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEE 360
           PEL++GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKE TLRNIA SEGVKFR A EEEE
Sbjct: 301 PELLSGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEVTLRNIASSEGVKFRAAGEEEE 360

Query: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420
           GM EARDLIERLLVKDPA RLGS KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK R
Sbjct: 361 GMGEARDLIERLLVKDPAMRLGSEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKGR 420

Query: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSSKNKTG 474
           S VSHV GV+ R+RRRGWWNWRK+ISN LIKNGS SKSH+NTN+YSYSSK KTG
Sbjct: 421 SQVSHVRGVSQRIRRRGWWNWRKVISNGLIKNGSCSKSHSNTNFYSYSSKKKTG 471

BLAST of Lsi07G008320 vs. NCBI nr
Match: NP_001267622.1 (protein kinase PINOID 2-like [Cucumis sativus] >KGN53527.1 hypothetical protein Csa_014467 [Cucumis sativus] >BAA82168.1 CsPK3 [Cucumis sativus] >BAA93704.1 cucumber protein kinase CsPK3 [Cucumis sativus])

HSP 1 Score: 855.5 bits (2209), Expect = 2.1e-244
Identity = 427/474 (90.08%), Postives = 443/474 (93.46%), Query Frame = 0

Query: 1   MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAV 60
           M+DDYSIFPPDSDLDFSFTST TDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA   
Sbjct: 1   MEDDYSIFPPDSDLDFSFTSTTTDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAA--- 60

Query: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120
           +NLRP+  SD HWSAI+AATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF
Sbjct: 61  LNLRPYHRSDSHWSAIRAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120

Query: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180
           ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS
Sbjct: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180

Query: 181 LLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240
           LLRKQPG+R SVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC
Sbjct: 181 LLRKQPGSRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240

Query: 241 FKSDVLPTFHTWTRPGPQATGCCFGWRTAEE-EDEIVGEFVAEPTAAFSKSCVGTHEYLA 300
           FKSDV+PTFHTWTRPGPQATG CFGWRT+ E E+EIVGEFVAEPT+AFSKSCVGTHEYLA
Sbjct: 241 FKSDVVPTFHTWTRPGPQATGSCFGWRTSPEFEEEIVGEFVAEPTSAFSKSCVGTHEYLA 300

Query: 301 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEE 360
           PELV GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA SEGVKFR   EEE 
Sbjct: 301 PELVTGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEGVKFRGGGEEEG 360

Query: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420
           GM EARDLIERLLVKDP RRLG  KGATEIKRHPFF+GIKWPLIRTYR PEVYG+MRK R
Sbjct: 361 GMGEARDLIERLLVKDPGRRLGWEKGATEIKRHPFFEGIKWPLIRTYRVPEVYGMMRKGR 420

Query: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSSKNKTG 474
           + VSHV  VT R+RRRGWWNWRK++  RLIKNGS SKSH+NTNYYSYSSK KTG
Sbjct: 421 AQVSHVREVTERIRRRGWWNWRKVMRKRLIKNGSCSKSHSNTNYYSYSSKKKTG 471

BLAST of Lsi07G008320 vs. NCBI nr
Match: XP_023553017.1 (serine/threonine-protein kinase WAG1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 851.3 bits (2198), Expect = 4.0e-243
Identity = 429/470 (91.28%), Postives = 443/470 (94.26%), Query Frame = 0

Query: 1   MDDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAV 60
           M+DDYS FPPDSDLDFSFTST TDRTF  SSSARTSLARSSLTLSFNESRL+AAAAAAA 
Sbjct: 1   MEDDYSFFPPDSDLDFSFTSTTTDRTF-ASSSARTSLARSSLTLSFNESRLAAAAAAAAA 60

Query: 61  VNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASF 120
           VN RPH HSDPHWSAIKAAT LSSDGHLHLRHLKL+RHLGTGNLGRVFLCHLRDNDHASF
Sbjct: 61  VNHRPHHHSDPHWSAIKAATALSSDGHLHLRHLKLVRHLGTGNLGRVFLCHLRDNDHASF 120

Query: 121 ALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180
           ALKVVDK+ALSNKKLLQVQTE+EILASL+HPFLPTLYARLDVSHYTCLLIDYCPAGDLHS
Sbjct: 121 ALKVVDKDALSNKKLLQVQTESEILASLNHPFLPTLYARLDVSHYTCLLIDYCPAGDLHS 180

Query: 181 LLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240
           LLRKQP  R SVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC
Sbjct: 181 LLRKQPNYRFSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLC 240

Query: 241 FKSDVLPTFHTWTRPGPQATGCCFGWRT-AEEEDEIVGEFVAEPTAAFSKSCVGTHEYLA 300
           FKS+V+PTFHTWT+PGPQ+T  CFGWRT A E  EIVGEFVAEPT AFSKSCVGTHEYLA
Sbjct: 241 FKSEVVPTFHTWTQPGPQSTS-CFGWRTAAAESQEIVGEFVAEPTTAFSKSCVGTHEYLA 300

Query: 301 PELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEEEE 360
           PELV GGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIA SE VKFR AA EEE
Sbjct: 301 PELVTGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIASSEAVKFR-AAVEEE 360

Query: 361 GMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420
           GMAEARDL+ERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR
Sbjct: 361 GMAEARDLVERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEVYGLMRKAR 420

Query: 421 SHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSSK 470
           SHVSHVTGV+HRMRRRGWWNWRKL SN L KNG++SKSHTN NYYSYSSK
Sbjct: 421 SHVSHVTGVSHRMRRRGWWNWRKL-SNGLFKNGNASKSHTNNNYYSYSSK 466

BLAST of Lsi07G008320 vs. TAIR 10
Match: AT1G53700.1 (WAG 1 )

HSP 1 Score: 596.3 bits (1536), Expect = 2.2e-170
Identity = 313/481 (65.07%), Postives = 367/481 (76.30%), Query Frame = 0

Query: 2   DDDYSIFPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAA----AAA 61
           DD Y +   D+DLD SFTSTATDRTF TSSSAR+SLARSSLTLSFN+ RLS A       
Sbjct: 3   DDGYYL---DTDLDLSFTSTATDRTF-TSSSARSSLARSSLTLSFND-RLSTATTPSTTT 62

Query: 62  AAVVNLRPHRHSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRD-ND 121
           ++      HR  DPHW++I+AATTLSSDG LHLRH KL+RHLGTGNLGRVFLCHLRD  +
Sbjct: 63  SSAATTLHHRRYDPHWTSIRAATTLSSDGRLHLRHFKLVRHLGTGNLGRVFLCHLRDCPN 122

Query: 122 HASFALKVVDKEALSNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAG 181
              FALKV+D++ L+ KK+  V+TEAEIL+ LDHPFLPTLYAR+D SHYTCLLIDYCP G
Sbjct: 123 PTGFALKVIDRDVLTAKKISHVETEAEILSLLDHPFLPTLYARIDASHYTCLLIDYCPNG 182

Query: 182 DLHSLLRKQPGNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSD 241
           DLHSLLRKQP NRL ++  RFFAAEVLVALEYLHALGIVYRDLKPEN+L+REDGH+MLSD
Sbjct: 183 DLHSLLRKQPNNRLPISPVRFFAAEVLVALEYLHALGIVYRDLKPENILIREDGHIMLSD 242

Query: 242 FDLCFKSDVLPTFHT----WTRPGPQAT---GCCFGWRTAEEEDEIVGEFVAEPTAAFSK 301
           FDLCFK+DV+PTF +     T   P+ T   G CF      E +EIV EF AEP  AFSK
Sbjct: 243 FDLCFKADVVPTFRSRRFRRTSSSPRKTRRGGGCFSTEVEYEREEIVAEFAAEPVTAFSK 302

Query: 302 SCVGTHEYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGV 361
           SCVGTHEYLAPELVAG GHG+GVDWWAFG+F+YE+L+GTTPFKG  KE TLRNI  ++ V
Sbjct: 303 SCVGTHEYLAPELVAGNGHGSGVDWWAFGIFLYEMLYGTTPFKGGTKEQTLRNIVSNDDV 362

Query: 362 KFRPAAEEEEGMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAP 421
            F     EEEGM EA+DLIE+LLVKDP +RLG  +GA +IKRH FF+GIKWPLIR Y+ P
Sbjct: 363 AF---TLEEEGMVEAKDLIEKLLVKDPRKRLGCARGAQDIKRHEFFEGIKWPLIRNYKPP 422

Query: 422 EVYGLMRKARSHVSHVT-GVTHRMRRRGWWNWRKLISNRLIKNGSSSKSHTNTNYYSYSS 470
           E+ GL++K ++H  HVT  VT R  +  WW    L+ ++ +   SSSK  +N NYY Y  
Sbjct: 423 EIRGLVKKTKAHAGHVTAAVTPRRNKWLWWALSHLLRSKSLSK-SSSKIQSNNNYYHYVG 474

BLAST of Lsi07G008320 vs. TAIR 10
Match: AT3G14370.1 (Protein kinase superfamily protein )

HSP 1 Score: 547.0 bits (1408), Expect = 1.5e-155
Identity = 305/480 (63.54%), Postives = 359/480 (74.79%), Query Frame = 0

Query: 10  PDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNE--SRLSAAAAAAAVVNLRPHR 69
           PD+DLD SFTST TDRTF +SS      AR+SLTLSFN+  S  SA   ++   +   HR
Sbjct: 9   PDTDLDLSFTSTTTDRTFASSS------ARTSLTLSFNDRLSTSSAVTTSSTSSSSVNHR 68

Query: 70  HSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDK 129
             DPHWSAIK+A  LSSDG++HLRHLKLIRHLGTGNLGRVFLC+LRD+  A FALKV+D+
Sbjct: 69  RHDPHWSAIKSAKLLSSDGNIHLRHLKLIRHLGTGNLGRVFLCNLRDSS-ARFALKVIDR 128

Query: 130 EAL-SNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQP 189
             L + KKL QV+TEAEIL+ LDHPFLPTLYAR+D SHYTCLLIDY P GDLHSLLRKQP
Sbjct: 129 NCLTTEKKLSQVETEAEILSLLDHPFLPTLYARIDESHYTCLLIDYAPNGDLHSLLRKQP 188

Query: 190 GNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVL 249
           GNRL +   RFFAAEVLVALEYLHA+GIVYRDLKPENVLLREDGHVMLSDFDLCFKSDV+
Sbjct: 189 GNRLPIQPVRFFAAEVLVALEYLHAMGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVV 248

Query: 250 PTFHT--WTRPGPQAT-----GCCFGWRTAE--EEDEIVGEFVAEPTAAFSKSCVGTHEY 309
           PTF +  + R     +       CF     +  E +EIV EF AEP  AFS+SCVGTHEY
Sbjct: 249 PTFKSRRYRRSSSSPSLRRRRSGCFSVAAEKKYEREEIVSEFAAEPVTAFSRSCVGTHEY 308

Query: 310 LAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFRPAAEE 369
           LAPELV+G GHG+GVDWWAFG+F+YELL+GTTPFKG +KE TLRNI  +     + A+  
Sbjct: 309 LAPELVSGNGHGSGVDWWAFGIFLYELLYGTTPFKGESKEQTLRNIVSTT----KTASFH 368

Query: 370 EEG-MAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAP-EVYGLM 429
            +G + EARDLIE+LLVKDP +RLG  +GA +IKRHPFFDGIKWPLIR Y+ P EV GL+
Sbjct: 369 MDGDLDEARDLIEKLLVKDPRKRLGCARGAQDIKRHPFFDGIKWPLIRHYKPPEEVRGLV 428

Query: 430 --RKARSHVSHVTGVTHRMRRRGWWNWRKLISNRLIKNGSS---SKSHTNTNYYSYSSKN 471
             +  R H SHV  V+   RRR  + WR L  + L++  SS   SK+ +N+NYY Y  K+
Sbjct: 429 IKKSTRPHASHVIAVS--PRRRKSFLWRAL--SYLLRGKSSSGGSKNQSNSNYYHYVGKS 473

BLAST of Lsi07G008320 vs. TAIR 10
Match: AT2G44830.1 (Protein kinase superfamily protein )

HSP 1 Score: 331.3 bits (848), Expect = 1.3e-90
Identity = 197/442 (44.57%), Postives = 259/442 (58.60%), Query Frame = 0

Query: 12  SDLDFSFTSTATDRTFT----TSSSARTSLARSSLTLSFNESRLSAAAAAAAVVNLRPHR 71
           SD+  S      DR+ +    +  S +TS +R+S +   +E   S+ +     +N +PH+
Sbjct: 283 SDVVLSMDKNYFDRSISMVLDSFESTKTSASRASDSSGLSEE--SSWSNFTGSLN-KPHK 342

Query: 72  HSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDK 131
            +DP W+AI A  T   DG L + H KL++ LG G++G V+L  L       FA+KV+DK
Sbjct: 343 GNDPWWNAILAIRT--RDGILGMSHFKLLKRLGCGDIGSVYLAEL-SGTRCHFAVKVMDK 402

Query: 132 EALSN-KKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQP 191
            +L + KKL + QTE +IL  LDHPFLPTLY   +   ++CL+++YCP GDLH+L ++QP
Sbjct: 403 ASLEDRKKLNRAQTERDILQLLDHPFLPTLYTHFETDRFSCLVMEYCPGGDLHTLRQRQP 462

Query: 192 GNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVL 251
           G   S  AARF+AAEVL+ALEYLH LG+VYRDLKPENVL+R+DGH+MLSDFDL  +  V 
Sbjct: 463 GKHFSEYAARFYAAEVLLALEYLHMLGVVYRDLKPENVLVRDDGHIMLSDFDLSLRCAVS 522

Query: 252 PTFHTWTRPGPQATGC-------------------CFGWRTA------------------ 311
           PT        P   G                    CF  R+                   
Sbjct: 523 PTLIKTFDSDPSRRGAFCVQPACMEPTSACIIQPSCFLPRSIFPNKNKKNKSRKTQADFF 582

Query: 312 EEEDEIVGEFVAEPTAAFSKSCVGTHEYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTT 371
           +     + E VAEP    S S VGTHEYLAPE++ G GHG+ VDWW FG+FV+ELL+G T
Sbjct: 583 KSHSGSLPELVAEPNTR-SMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFVHELLYGKT 642

Query: 372 PFKGVNKEGTLRNIAMSEGVKFRPAAEEEEGMAEARDLIERLLVKDPARRLGSGKGATEI 412
           PFKG     TL N+ + E +KF    E        RDLI+ LLVKDP  RLG+ +GATEI
Sbjct: 643 PFKGSGNRATLFNV-VGEQLKF---PESPATSYAGRDLIQALLVKDPKNRLGTKRGATEI 702

BLAST of Lsi07G008320 vs. TAIR 10
Match: AT5G47750.1 (D6 protein kinase like 2 )

HSP 1 Score: 330.1 bits (845), Expect = 2.9e-90
Identity = 186/419 (44.39%), Postives = 251/419 (59.90%), Query Frame = 0

Query: 32  SARTSLARSSLTLSFNESRLSAAAAAAAVVNLRPHRHSDPHWSAIKAATTLSSDGHLHLR 91
           S ++S+ R S +   ++   S+ ++ ++ VN +PH+ +D  W AI+A      DG L L 
Sbjct: 135 SGKSSICRGSTSSDVSDE--SSCSSFSSTVN-KPHKANDLRWEAIQAVRV--RDGLLGLS 194

Query: 92  HLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDKEAL-SNKKLLQVQTEAEILASLDH 151
           H +L++ LG G++G V+L  L       FA+KV+DK +L S KKLL+ QTE EIL SLDH
Sbjct: 195 HFRLLKRLGCGDIGSVYLSEL-SGTKCYFAMKVMDKTSLASRKKLLRAQTEREILQSLDH 254

Query: 152 PFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQPGNRLSVAAARFFAAEVLVALEYLH 211
           PFLPTLY   +   ++CL++++CP GDLH+L ++QPG   S  A +F+ AE L+ALEYLH
Sbjct: 255 PFLPTLYTHFETEKFSCLVMEFCPGGDLHTLRQRQPGKHFSEQAVKFYIAESLLALEYLH 314

Query: 212 ALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVLPTF--------------------- 271
            LGIVYRDLKPENVL+REDGH+MLSDFDL  +  V PT                      
Sbjct: 315 MLGIVYRDLKPENVLVREDGHIMLSDFDLSLRCLVSPTLVKSAAIESDPLRKNVYCVQPA 374

Query: 272 ---HTWTRPGPQATGCCFGWRTAEEEDE--------------IVGEFVAEPTAAFSKSCV 331
               +  +P       CF  R    + +               + E VAEPT A S S V
Sbjct: 375 CIEPSCIQPSCTVPTTCFSPRLFSSKSKKDRKPKNDTANQVRPLPELVAEPTDARSMSFV 434

Query: 332 GTHEYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPFKGVNKEGTLRNIAMSEGVKFR 391
           GTHEYLAPE++ G GHG+ VDWW FG+F+YELL G TPFKG     TL N+ + + ++F 
Sbjct: 435 GTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGRTPFKGSGNRQTLFNV-VGQPLRF- 494

Query: 392 PAAEEEEGMAEARDLIERLLVKDPARRLGSGKGATEIKRHPFFDGIKWPLIRTYRAPEV 412
              E       ARDLI  LL+K+P +RLG  +GATE+K+HPFF+G+ W LIR    PE+
Sbjct: 495 --PETPVVSFAARDLIRGLLMKEPQQRLGFKRGATEVKQHPFFEGVNWALIRCATPPEI 543

BLAST of Lsi07G008320 vs. TAIR 10
Match: AT4G26610.1 (D6 protein kinase like 1 )

HSP 1 Score: 327.4 bits (838), Expect = 1.9e-89
Identity = 190/440 (43.18%), Postives = 258/440 (58.64%), Query Frame = 0

Query: 8   FPPDSDLDFSFTSTATDRTFTTSSSARTSLARSSLTLSFNESRLSAAAAAAAVVNLRPHR 67
           F P SD   S T         +  S +++  R S +   ++    ++ +     N +PH+
Sbjct: 47  FAPSSD---SKTGGEVKFNEKSDQSGKSNTCRPSTSSDISDESTCSSFSG----NNKPHK 106

Query: 68  HSDPHWSAIKAATTLSSDGHLHLRHLKLIRHLGTGNLGRVFLCHLRDNDHASFALKVVDK 127
            +D  W AI+A  T    G L L H +L++ LG G++G V L  L       FA+KV+DK
Sbjct: 107 ANDVRWEAIQAVRT--KHGVLGLNHFRLLKRLGCGDIGTVHLAELH-GTRCFFAMKVMDK 166

Query: 128 EAL-SNKKLLQVQTEAEILASLDHPFLPTLYARLDVSHYTCLLIDYCPAGDLHSLLRKQP 187
            AL S KKLL+ QTE EIL  LDHPFLPTLY+  +   ++CL++++CP GDLH+L ++QP
Sbjct: 167 GALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEFCPGGDLHTLRQRQP 226

Query: 188 GNRLSVAAARFFAAEVLVALEYLHALGIVYRDLKPENVLLREDGHVMLSDFDLCFKSDVL 247
           G R S  AA+F+ AEVL+A+EYLH LGI+YRDLKPENVL+R+DGHVMLSDFDL  +  V 
Sbjct: 227 GKRFSEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHVMLSDFDLSLRCTVS 286

Query: 248 PTFHTWT---RPGPQATGCC-----------------------FGWRTAEEED------- 307
           PT    T     G + +G C                       F  ++ +++        
Sbjct: 287 PTVVRSTVLASEGQKNSGYCAQPACIQQPSCISAPTTCFSPRYFSSKSKKDKKMKNETGN 346

Query: 308 --EIVGEFVAEPTAAFSKSCVGTHEYLAPELVAGGGHGNGVDWWAFGVFVYELLHGTTPF 367
               + E VAEPT+A S S VGTHEYLAPE++ G GHG+ VDWW FG+F+YELL G TPF
Sbjct: 347 QVSPLPELVAEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGKTPF 406

Query: 368 KGVNKEGTLRNIAMSEGVKFRPAAEEEEGMAEARDLIERLLVKDPARRLGSGKGATEIKR 412
           KG     TL N+ + + ++F    E       ARDLI  LLVK+P  RL   +GATE+K+
Sbjct: 407 KGSGNRATLFNV-VGQPLRF---PESPVVSFAARDLIRSLLVKEPQHRLAYKRGATEMKQ 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C8M53.1e-16965.07Serine/threonine-protein kinase WAG1 OS=Arabidopsis thaliana OX=3702 GN=WAG1 PE=... [more]
Q9LUL22.1e-15463.54Serine/threonine-protein kinase WAG2 OS=Arabidopsis thaliana OX=3702 GN=WAG2 PE=... [more]
Q2QM772.3e-9247.72Protein kinase PINOID OS=Oryza sativa subsp. japonica OX=39947 GN=PID PE=2 SV=1[more]
Q94E492.2e-9042.79Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV... [more]
P157922.4e-8943.49Protein kinase PVPK-1 OS=Phaseolus vulgaris OX=3885 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3D1P23.4e-24890.93Serine/threonine-protein kinase WAG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BRT13.4e-24890.93serine/threonine-protein kinase WAG1 OS=Cucumis melo OX=3656 GN=LOC103492987 PE=... [more]
A0A5A7V4J38.5e-24790.51Serine/threonine-protein kinase WAG1 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Q9XGL21.0e-24490.08CsPK3 OS=Cucumis sativus OX=3659 GN=CsPK3 PE=2 SV=1[more]
A0A6J1J9F82.4e-24191.42serine/threonine-protein kinase WAG1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_038899976.15.8e-26696.84serine/threonine-protein kinase WAG1-like [Benincasa hispida][more]
XP_008451816.17.1e-24890.93PREDICTED: serine/threonine-protein kinase WAG1 [Cucumis melo] >TYK16379.1 serin... [more]
KAA0062963.11.8e-24690.51serine/threonine-protein kinase WAG1 [Cucumis melo var. makuwa][more]
NP_001267622.12.1e-24490.08protein kinase PINOID 2-like [Cucumis sativus] >KGN53527.1 hypothetical protein ... [more]
XP_023553017.14.0e-24391.28serine/threonine-protein kinase WAG1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G53700.12.2e-17065.07WAG 1 [more]
AT3G14370.11.5e-15563.54Protein kinase superfamily protein [more]
AT2G44830.11.3e-9044.57Protein kinase superfamily protein [more]
AT5G47750.12.9e-9044.39D6 protein kinase like 2 [more]
AT4G26610.11.9e-8943.18D6 protein kinase like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 354..374
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 93..176
e-value: 1.5E-42
score: 147.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 288..408
e-value: 1.7E-32
score: 114.6
coord: 177..242
e-value: 1.5E-42
score: 147.6
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 97..237
e-value: 1.1E-6
score: 25.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 451..473
NoneNo IPR availablePANTHERPTHR45637:SF2SERINE/THREONINE-PROTEIN KINASE WAG1coord: 10..425
NoneNo IPR availablePANTHERPTHR45637FLIPPASE KINASE 1-RELATEDcoord: 10..425
NoneNo IPR availableCDDcd05574STKc_phototropin_likecoord: 91..411
e-value: 1.03832E-159
score: 452.847
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 93..395
e-value: 6.7E-77
score: 271.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 95..395
e-value: 1.0E-53
score: 182.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 93..395
score: 43.22673
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 214..226
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 69..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G008320.1Lsi07G008320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity