Lsi05G018750.1 (mRNA) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G018750.1
TypemRNA
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptioncytochrome c oxidase-assembly factor COX23, mitochondrial
Locationchr05: 25952645 .. 25955415 (+)
Sequence length520
RNA-Seq ExpressionLsi05G018750.1
SyntenyLsi05G018750.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGGTCCTCTCGATACAATTACGTATAAAAGGAGATTGATTCTCTTGCTTTCTCATCGGAATTTCAGGCAGCCATTTTAACCGACCTCTATTTTTCCACCGAGACAGAGTGAGCTTTTCCGCCATGGCTTCCACTTCTAGGGATCCGGCTCCGCCGTACGCCAGTGCTGCTAGAATTTCCGATTCTCCTTGTTATCCTCAGTACAGCGCTTCCCTGAAATGTAGGTTTCACTCTCGTACTCCCTCTCCATTATTCTCAATTTTTTTCGTTTTGCTTTGATTCTCCGCCAAATATTTACGTGTCTGTGCTACCATTATCTCACCATTTGGACTTCTTCTACACTGGTTCACTTGAGAATGATTGCGTGCTGCTGTGCATCAATTTTTTCAGTTTGTGTTCTGGATGGTTAAACTCTCGAATTTGTTGTTGGCTGTGATCGTGCAAACTCTAATTTTCGCGGCGATTCTCTATGCTCTGTTAGTTGAATAATTGGTTAGCCTGTTTTATTTCATTTTCTAGGGATGCTATTTCCATCCAATTTAGGTCAATTGATGAAGAGGAAGCAAACTTAAATGTATTATTGATCGGATGATAATCTTAAAAACAAAATAGGGTTTACCAGGGTATTAGTTGATAGAGCGCTCGTGAGAGTTCTTAGAGTGGCTATTCTAACTATGGCAATGGTGGAAAAGGAACTTTTACTAGGATATTGAAGCCGAAAGCTCTTAATCCCCCGTTCCTTAGGGCCAAGTTCTTATTTAAAATATCAAAGTAGGAAAGAATGTTAGATGTTTAATGCAGAAGCCTCGAGCTGCAACTTAAGGAAAGATTGTTAACGGAAAGCATGCATAACTGGTAATGGCTGATAAATCAGGTGTAAAGTGAAATTTTAATTTGAATTTGTAAATGAGTACTATCGTTATTGATCCTGATAAAGTGTAGCTTAATCTTTGGTGTGTTATAAATCATCCAATGAGAAGTTGGTGTTTAGAATACTAATTTTGTAGATTTTGAATTTTATTTTTATGTCAGAGAACATTTTGAACTTCTTATCAAAGAAACTCTCTTTTCCAAATGCTATAGGTCTTGAAGAACATAGCTCCGAGAAGAGCAAATGTCAAGAACATTTTGATGTTTACAAGGAATGCAAGAAGAAAGAGGTATGTTCTTTTCTTAGTTATCACATGAAATTGGAATGGAGGAACATTTCATCGTGTGTGGTACTGTTACTGGAAGCATACACTCACTATTTATGGGAATTTGACTTAAACTGAGATGATGGTACTAACTAACCAAGGTTGCAATTGCTCTGATTTGACTAGTTACTTAACTTGTTCTGTTTTTTGCCTCTTTGGAGTCTGCTCGTTTGCTTGTGACGTTAATGGATCAAGGAAATTTAAACAATATCCTCATCTCATTTGGACATAATTGGATAACCAGTGTGTCATTGAGTTAGTTATGTGCTCAATCTTAAAGGAATGTGTTAAAATATGATTTTGGTCCTCACAAGAAGTTCTATTTTGATCTTTCAAATTTTAGTAATTTCTATATAAGATATTCTATCCTAGTTAAAATCTGTAACGAAGAAGTGACTGGACTAATTTTCTGATATCAGATTTATATGCAAGTGATGTATTACATGTCTATGTTGTCTCTTCCATGTGATAACCTTTTTTTAGTTATATGTTGATCTGCAACAATATAGAATGCCATTTATTTTTTGTATGCAAGTCTTTGACTCAGATTTTAATGATAGATTGAATTTGAAATTATTGAAGCTTGAGGGTCTAACATAAAAACTTTTTGGAACTTTGGGTTTTAACTTCAAGAATTAAAATTACTCTATGACCTGGAAGATACATGGCATATAAGGTGAGAAGTATATGTGGTTCATGATACTATGGAAACTTTAAAACCCTCTTCCTGTGTATTCCATAACATTTATGAATCCTATCTGGGTTTAGTAGATATAGAAATGTTCCATAGGCTGTCATTTAATTCCTTGGTTAGTAGAGTAGCATCATGCACCAACTCTTAATAAGACATTGCCTCGTAACGCGACTGAGATCTGTCTCCTTTACAGCGGGAAGATCGATTGGAACGCAACAAGAAACGGTCTTTCTTCTCATGAAATTGTTTCTTGATGTTGAAGTTATGTAAGTTTAATATACAATCTACACACGTTTATTAACCTTCTTGTAGCAGTAGATTTATCTGGTGCCTCATTGAGACAATGACTCGGCATGTATTGCCCACTTACTATCATTCGTCTATTAATAAAAGATGCATACCATCTTTATGTTCAATTATCTCCATGTTGATCAATTTTCATTCTTAAAGTGTAGGAATCTGTAAACATTGATCCTCCATTTGATCTAAATAGTGCAGGCTACAATCACGATTAACCCGTTAGGCGGTCAAGAATTTACGACAGCAGTTGAGGTATTTACCTTTTTTAAGAGGGCGTACGTTAAAAGCTCCGATGTTATATGATAGTTCTATGATATACAATGATTTCTAACTTTTAAGTTGAAAATACAATGTTTTATTCGATTTGATACTTTTTTAAAAAAGGGTTTAAAATTCAAATCTCCCAACCAATATATGAAGAAATGACTGATATTGATTTGAGTCTTATTTGATAGGCTATTGATTATATTAGAAGTTTATAGTGAAGATGAATTATAATTCAAACCACCCATTTTCTGCTCAATGATGATCGTATGCCTTGAACTTATGTTCACATTTGGTTTATTCTATGAAATGTCTACC

mRNA sequence

GGGTCCTCTCGATACAATTACGTATAAAAGGAGATTGATTCTCTTGCTTTCTCATCGGAATTTCAGGCAGCCATTTTAACCGACCTCTATTTTTCCACCGAGACAGAGTGAGCTTTTCCGCCATGGCTTCCACTTCTAGGGATCCGGCTCCGCCGTACGCCAGTGCTGCTAGAATTTCCGATTCTCCTTGTTATCCTCAGTACAGCGCTTCCCTGAAATGTCTTGAAGAACATAGCTCCGAGAAGAGCAAATGTCAAGAACATTTTGATGTTTACAAGGAATGCAAGAAGAAAGAGGAATCTGTAAACATTGATCCTCCATTTGATCTAAATAGTGCAGGCTACAATCACGATTAACCCGTTAGGCGGTCAAGAATTTACGACAGCAGTTGAGGCTATTGATTATATTAGAAGTTTATAGTGAAGATGAATTATAATTCAAACCACCCATTTTCTGCTCAATGATGATCGTATGCCTTGAACTTATGTTCACATTTGGTTTATTCTATGAAATGTCTACC

Coding sequence (CDS)

ATGGCTTCCACTTCTAGGGATCCGGCTCCGCCGTACGCCAGTGCTGCTAGAATTTCCGATTCTCCTTGTTATCCTCAGTACAGCGCTTCCCTGAAATGTCTTGAAGAACATAGCTCCGAGAAGAGCAAATGTCAAGAACATTTTGATGTTTACAAGGAATGCAAGAAGAAAGAGGAATCTGTAAACATTGATCCTCCATTTGATCTAAATAGTGCAGGCTACAATCACGATTAA

Protein sequence

MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEESVNIDPPFDLNSAGYNHD
Homology
BLAST of Lsi05G018750.1 vs. ExPASy Swiss-Prot
Match: P38824 (Cytochrome c oxidase-assembly factor COX23, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=COX23 PE=1 SV=1)

HSP 1 Score: 43.9 bits (102), Expect = 9.6e-04
Identity = 18/36 (50.00%), Postives = 25/36 (69.44%), Query Frame = 0

Query: 22  PCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKK 58
           PC      S +CLE +  ++SKCQE+FD Y+ECKK+
Sbjct: 103 PCEESSKLSFQCLERNDYDRSKCQEYFDAYRECKKQ 138

BLAST of Lsi05G018750.1 vs. ExPASy TrEMBL
Match: A0A5A7U004 (Cytochrome c oxidase-assembly factor COX23 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003050 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.1e-22
Identity = 56/64 (87.50%), Postives = 59/64 (92.19%), Query Frame = 0

Query: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEES 60
          MASTSRDPAPPYASAARISDSPCYPQYSASLKCLE +SSEKSKCQEHFDVYKECKKKE  
Sbjct: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEVNSSEKSKCQEHFDVYKECKKKERE 60

Query: 61 VNID 65
            ++
Sbjct: 61 ARLE 64

BLAST of Lsi05G018750.1 vs. ExPASy TrEMBL
Match: A0A6J1D157 (cytochrome c oxidase-assembly factor COX23, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016071 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.1e-22
Identity = 55/64 (85.94%), Postives = 59/64 (92.19%), Query Frame = 0

Query: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEES 60
          MAST+RD APPYASAARISDSPCYPQYSASLKCLEEHSSEK+KCQEHFDVYKECKKKE  
Sbjct: 1  MASTTRDSAPPYASAARISDSPCYPQYSASLKCLEEHSSEKNKCQEHFDVYKECKKKERE 60

Query: 61 VNID 65
            ++
Sbjct: 61 ARLE 64

BLAST of Lsi05G018750.1 vs. ExPASy TrEMBL
Match: A0A6J1E9H8 (cytochrome c oxidase-assembly factor COX23, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111432060 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 2.8e-22
Identity = 54/64 (84.38%), Postives = 59/64 (92.19%), Query Frame = 0

Query: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEES 60
          MASTSRDP+PPY SAARISDSPC+PQYSASLKCLEEHSSE+SKCQEHFDVYKECKKKE  
Sbjct: 1  MASTSRDPSPPYPSAARISDSPCFPQYSASLKCLEEHSSERSKCQEHFDVYKECKKKERE 60

Query: 61 VNID 65
            ++
Sbjct: 61 ARLE 64

BLAST of Lsi05G018750.1 vs. ExPASy TrEMBL
Match: A0A0A0L4K5 (CHCH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129550 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.6e-22
Identity = 55/64 (85.94%), Postives = 57/64 (89.06%), Query Frame = 0

Query: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEES 60
          MASTSRDP PPY SAARISDSPCYPQYSASLKCLE HSSEKSKCQEHFDVYKECKKKE  
Sbjct: 1  MASTSRDPTPPYPSAARISDSPCYPQYSASLKCLEVHSSEKSKCQEHFDVYKECKKKERE 60

Query: 61 VNID 65
            ++
Sbjct: 61 ARLE 64

BLAST of Lsi05G018750.1 vs. ExPASy TrEMBL
Match: A0A6J1ITM4 (cytochrome c oxidase-assembly factor COX23, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111479820 PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 6.9e-21
Identity = 53/64 (82.81%), Postives = 57/64 (89.06%), Query Frame = 0

Query: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEES 60
          MASTSRDP PPY SAARISDSPC+ QYSASLKCLEEHSSE+SKCQEHFDVYKECKKKE  
Sbjct: 1  MASTSRDPPPPYPSAARISDSPCFLQYSASLKCLEEHSSERSKCQEHFDVYKECKKKERE 60

Query: 61 VNID 65
            ++
Sbjct: 61 ARLE 64

BLAST of Lsi05G018750.1 vs. NCBI nr
Match: XP_038906947.1 (cytochrome c oxidase-assembly factor COX23, mitochondrial [Benincasa hispida])

HSP 1 Score: 117.1 bits (292), Expect = 6.8e-23
Identity = 56/64 (87.50%), Postives = 60/64 (93.75%), Query Frame = 0

Query: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEES 60
          MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEH+SEKSKC+EHFDVYKECKKKE  
Sbjct: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHNSEKSKCREHFDVYKECKKKERE 60

Query: 61 VNID 65
            ++
Sbjct: 61 ARLE 64

BLAST of Lsi05G018750.1 vs. NCBI nr
Match: XP_022147041.1 (cytochrome c oxidase-assembly factor COX23, mitochondrial [Momordica charantia])

HSP 1 Score: 114.4 bits (285), Expect = 4.4e-22
Identity = 55/64 (85.94%), Postives = 59/64 (92.19%), Query Frame = 0

Query: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEES 60
          MAST+RD APPYASAARISDSPCYPQYSASLKCLEEHSSEK+KCQEHFDVYKECKKKE  
Sbjct: 1  MASTTRDSAPPYASAARISDSPCYPQYSASLKCLEEHSSEKNKCQEHFDVYKECKKKERE 60

Query: 61 VNID 65
            ++
Sbjct: 61 ARLE 64

BLAST of Lsi05G018750.1 vs. NCBI nr
Match: KAA0049122.1 (cytochrome c oxidase-assembly factor COX23 [Cucumis melo var. makuwa] >TYK17441.1 cytochrome c oxidase-assembly factor COX23 [Cucumis melo var. makuwa])

HSP 1 Score: 114.4 bits (285), Expect = 4.4e-22
Identity = 56/64 (87.50%), Postives = 59/64 (92.19%), Query Frame = 0

Query: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEES 60
          MASTSRDPAPPYASAARISDSPCYPQYSASLKCLE +SSEKSKCQEHFDVYKECKKKE  
Sbjct: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEVNSSEKSKCQEHFDVYKECKKKERE 60

Query: 61 VNID 65
            ++
Sbjct: 61 ARLE 64

BLAST of Lsi05G018750.1 vs. NCBI nr
Match: XP_022924627.1 (cytochrome c oxidase-assembly factor COX23, mitochondrial [Cucurbita moschata] >KAG6582500.1 hypothetical protein SDJN03_22502, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018885.1 hypothetical protein SDJN02_20758 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 114.0 bits (284), Expect = 5.7e-22
Identity = 54/64 (84.38%), Postives = 59/64 (92.19%), Query Frame = 0

Query: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEES 60
          MASTSRDP+PPY SAARISDSPC+PQYSASLKCLEEHSSE+SKCQEHFDVYKECKKKE  
Sbjct: 1  MASTSRDPSPPYPSAARISDSPCFPQYSASLKCLEEHSSERSKCQEHFDVYKECKKKERE 60

Query: 61 VNID 65
            ++
Sbjct: 61 ARLE 64

BLAST of Lsi05G018750.1 vs. NCBI nr
Match: XP_004133972.1 (cytochrome c oxidase-assembly factor COX23, mitochondrial [Cucumis sativus] >KGN56698.1 hypothetical protein Csa_009870 [Cucumis sativus])

HSP 1 Score: 113.6 bits (283), Expect = 7.5e-22
Identity = 55/64 (85.94%), Postives = 57/64 (89.06%), Query Frame = 0

Query: 1  MASTSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEES 60
          MASTSRDP PPY SAARISDSPCYPQYSASLKCLE HSSEKSKCQEHFDVYKECKKKE  
Sbjct: 1  MASTSRDPTPPYPSAARISDSPCYPQYSASLKCLEVHSSEKSKCQEHFDVYKECKKKERE 60

Query: 61 VNID 65
            ++
Sbjct: 61 ARLE 64

BLAST of Lsi05G018750.1 vs. TAIR 10
Match: AT1G02160.1 (Cox19 family protein (CHCH motif) )

HSP 1 Score: 86.3 bits (212), Expect = 1.2e-17
Identity = 42/61 (68.85%), Postives = 49/61 (80.33%), Query Frame = 0

Query: 4  TSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKEESVNI 63
          +++  A  Y SAARISDSPCY QYSASLKCLEE  S+KSKCQ+HFDVYKECKKKE    +
Sbjct: 2  STKGAAAAYPSAARISDSPCYLQYSASLKCLEEFGSDKSKCQDHFDVYKECKKKEREARL 61

Query: 64 D 65
          +
Sbjct: 62 E 62

BLAST of Lsi05G018750.1 vs. TAIR 10
Match: AT1G02160.2 (Cox19 family protein (CHCH motif) )

HSP 1 Score: 84.7 bits (208), Expect = 3.5e-17
Identity = 42/55 (76.36%), Postives = 47/55 (85.45%), Query Frame = 0

Query: 4  TSRDPAPPYASAARISDSPCYPQYSASLKCLEEHSSEKSKCQEHFDVYKECKKKE 59
          +++  A  Y SAARISDSPCY QYSASLKCLEE  S+KSKCQ+HFDVYKECKKKE
Sbjct: 2  STKGAAAAYPSAARISDSPCYLQYSASLKCLEEFGSDKSKCQDHFDVYKECKKKE 56

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P388249.6e-0450.00Cytochrome c oxidase-assembly factor COX23, mitochondrial OS=Saccharomyces cerev... [more]
Match NameE-valueIdentityDescription
A0A5A7U0042.1e-2287.50Cytochrome c oxidase-assembly factor COX23 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A6J1D1572.1e-2285.94cytochrome c oxidase-assembly factor COX23, mitochondrial OS=Momordica charantia... [more]
A0A6J1E9H82.8e-2284.38cytochrome c oxidase-assembly factor COX23, mitochondrial OS=Cucurbita moschata ... [more]
A0A0A0L4K53.6e-2285.94CHCH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129550 PE=4 S... [more]
A0A6J1ITM46.9e-2182.81cytochrome c oxidase-assembly factor COX23, mitochondrial OS=Cucurbita maxima OX... [more]
Match NameE-valueIdentityDescription
XP_038906947.16.8e-2387.50cytochrome c oxidase-assembly factor COX23, mitochondrial [Benincasa hispida][more]
XP_022147041.14.4e-2285.94cytochrome c oxidase-assembly factor COX23, mitochondrial [Momordica charantia][more]
KAA0049122.14.4e-2287.50cytochrome c oxidase-assembly factor COX23 [Cucumis melo var. makuwa] >TYK17441.... [more]
XP_022924627.15.7e-2284.38cytochrome c oxidase-assembly factor COX23, mitochondrial [Cucurbita moschata] >... [more]
XP_004133972.17.5e-2285.94cytochrome c oxidase-assembly factor COX23, mitochondrial [Cucumis sativus] >KGN... [more]
Match NameE-valueIdentityDescription
AT1G02160.11.2e-1768.85Cox19 family protein (CHCH motif) [more]
AT1G02160.23.5e-1776.36Cox19 family protein (CHCH motif) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010625CHCHPFAMPF06747CHCHcoord: 23..57
e-value: 1.2E-8
score: 34.8
NoneNo IPR availablePANTHERPTHR48150CYTOCHROME C OXIDASE-ASSEMBLY FACTOR COX23, MITOCHONDRIALcoord: 3..60
NoneNo IPR availablePROSITEPS51808CHCHcoord: 20..62
score: 13.724955
IPR009069Cysteine alpha-hairpin motif superfamilySUPERFAMILY47072Cysteine alpha-hairpin motifcoord: 21..57

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lsi05G018750Lsi05G018750gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi05G018750.1.exon.1Lsi05G018750.1.exon.1exon
Lsi05G018750.1.exon.2Lsi05G018750.1.exon.2exon
Lsi05G018750.1.exon.3Lsi05G018750.1.exon.3exon
Lsi05G018750.1.exon.4Lsi05G018750.1.exon.4exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi05G018750.1.five_prime_UTR.1Lsi05G018750.1.five_prime_UTR.1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi05G018750.1.CDS.1Lsi05G018750.1.CDS.1CDS
Lsi05G018750.1.CDS.2Lsi05G018750.1.CDS.2CDS
Lsi05G018750.1.CDS.3Lsi05G018750.1.CDS.3CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lsi05G018750.1.three_prime_UTR.1Lsi05G018750.1.three_prime_UTR.1three_prime_UTR
Lsi05G018750.1.three_prime_UTR.2Lsi05G018750.1.three_prime_UTR.2three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lsi05G018750.1Lsi05G018750.1-proteinpolypeptide