Lsi05G011400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G011400
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr05: 19154644 .. 19156530 (-)
RNA-Seq ExpressionLsi05G011400
SyntenyLsi05G011400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAAACAGGCGTGTTTGATTCTGATCCTAAAGTTATGGGGAAAGCCACGGAGTTGAAGAGGGAGTTACAGAGACTTATCGTTGCTATAATTGATGACGATAATTATTCCACCGACACCATTGATAACGCTAAAGAGATTCTTTCTGCACTTAAGGAGTTGAAACTCAGGAAGAGATCGCGTTCTTCTTCTAATCTTTTTGAGAATCTCATTTGTCCTGAGGAATTTCGTTGTCCGCTTTCTAGAGAACTCATGAGAGATCCTGTCATTGTGTCTACTGGCGAGGTTTGTGATTATATATATATATATATATATATTTTTCCCTCCTCTTCTAGGTCTTGAAATTAATTCCTAATTCGTGGTCCTTGCTCCTTTGTTCTTGAGAATACGAGTGTGAAATTTAGTCGTCTTCTTCTTCTTCTTCAATCAGTGTTCTTGAGAAGGTTTCCAGGTTTCTATGGTTTTAGATTAGGATTTTCAATTTAACTTGCTGAATGTTCATTGACATTGCATCTTTTGTATTTAATTTTAGAATTTAGAGGGCGAACGATTCAAACCCTTAGCATCTTGAGTTACTGTGTATTCATGTTGTTGACTGTGAATAAATATGAATGGTTTACCTGGAATTCTATAGAATAGAGGAGGAGGAGAAGTTTTTTTTATAGATCTTTTGTTATATGTATGGTTTTGGGGGGACTGGATCTTATTGCCCATTATGAATTCTTTGTTTTCATTATGTTGGTTTTTGAGTTTTCTTGTTCTTATTGTTGTGTTTCATCTTATTTGCAGACATATGATAGACCTTTCATTCACAAATGGTTAAAATCAGGGAACAGGACTTGCCCCAGAACTCAGCAAGTTCTTTCACACACCAATCTCACTCCCAATCACTTGATTCGTGAAATGATATCTCAGTGGTGTACAAGTCGGGGAATTGAGTTGCAAGATCGTGTCCGTGTTCATTATCTTGACGATGATGTGATAACGGAGGCTGATCGAGACCGTTTTCTCACGTTAATAGAGAAAATGTCAATGACAATATCTGAACAGAAAGCAGCTGCTAAGGAGCTTCGAATGTTGACGAAGCGAATGCCCTCATTTCGAGCACTTTTTGGTGAGTCCCTTGATGCCATATCTTTGTTGCTTAGTCCACTTTGTGGAGATAAGTCACAAAGTATTAGTATTCACACGGATCTGCAAGAAGATGTGATCACAACCCTCTTGAATTTGTCGATCCATGACAACAACAAGAAGCTTGTTGCGGAAACACCCAAGGTAATACCTCTTCTAATGGAAGCGTTGAGATCAGGAACCATGGAAACAAGAAGCAATGCAGCTGCAGCCCTATTTACTCTGTCAGCTCTCGACTCAAACAAAGCGATCATCGGGAAAGCTGGTGCGTTGAAACCACTTATCGACCTTTTAGATGAGGGGCATCCTTTATCAATCAAAGATGCTGCTTCAGCAATTTTCAATCTTTGTATAATTCATGAAAACAAAGCAAGAGCTGTGCGAGATGGTGCAGTCCGAGTGCTTCTGAAAAAGATCATGAACCAGATGCATGTTGATGAGTTGTTGGCTATTCTTGCAATGTTATCAAGCCATCAAAAAGCTATCGAAGAAATTGGAGAACTTGGAGCTGTCCCTTTCTTACTCAGAATTATCAGGGAAAGTCCTTGTTCACGTAACAAGGAGAATTGCATTGTGATCATCCATGCTATCTGCTTATACGATCGAACCAAGTGGAAGGATATGAGGGAGGAAGAGAAATGTTATCGAACTATATCTGAACTTGCTCAGAATGGAACTTCAAGGGCCAAGAGAAAGGCCAGTGGCATACTTGAAAGACTAAACAGGGTAGTTAATATGACCCATACCGCCTAA

mRNA sequence

ATGGCGAAAACAGGCGTGTTTGATTCTGATCCTAAAGTTATGGGGAAAGCCACGGAGTTGAAGAGGGAGTTACAGAGACTTATCGTTGCTATAATTGATGACGATAATTATTCCACCGACACCATTGATAACGCTAAAGAGATTCTTTCTGCACTTAAGGAGTTGAAACTCAGGAAGAGATCGCGTTCTTCTTCTAATCTTTTTGAGAATCTCATTTGTCCTGAGGAATTTCGTTGTCCGCTTTCTAGAGAACTCATGAGAGATCCTGTCATTGTGTCTACTGGCGAGACATATGATAGACCTTTCATTCACAAATGGTTAAAATCAGGGAACAGGACTTGCCCCAGAACTCAGCAAGTTCTTTCACACACCAATCTCACTCCCAATCACTTGATTCGTGAAATGATATCTCAGTGGTGTACAAGTCGGGGAATTGAGTTGCAAGATCGTGTCCGTGTTCATTATCTTGACGATGATGTGATAACGGAGGCTGATCGAGACCGTTTTCTCACGTTAATAGAGAAAATGTCAATGACAATATCTGAACAGAAAGCAGCTGCTAAGGAGCTTCGAATGTTGACGAAGCGAATGCCCTCATTTCGAGCACTTTTTGGTGAGTCCCTTGATGCCATATCTTTGTTGCTTAGTCCACTTTGTGGAGATAAGTCACAAAGTATTAGTATTCACACGGATCTGCAAGAAGATGTGATCACAACCCTCTTGAATTTGTCGATCCATGACAACAACAAGAAGCTTGTTGCGGAAACACCCAAGGTAATACCTCTTCTAATGGAAGCGTTGAGATCAGGAACCATGGAAACAAGAAGCAATGCAGCTGCAGCCCTATTTACTCTGTCAGCTCTCGACTCAAACAAAGCGATCATCGGGAAAGCTGGTGCGTTGAAACCACTTATCGACCTTTTAGATGAGGGGCATCCTTTATCAATCAAAGATGCTGCTTCAGCAATTTTCAATCTTTGTATAATTCATGAAAACAAAGCAAGAGCTGTGCGAGATGGTGCAGTCCGAGTGCTTCTGAAAAAGATCATGAACCAGATGCATGTTGATGAGTTGTTGGCTATTCTTGCAATGTTATCAAGCCATCAAAAAGCTATCGAAGAAATTGGAGAACTTGGAGCTGTCCCTTTCTTACTCAGAATTATCAGGGAAAGTCCTTGTTCACGTAACAAGGAGAATTGCATTGTGATCATCCATGCTATCTGCTTATACGATCGAACCAAGTGGAAGGATATGAGGGAGGAAGAGAAATGTTATCGAACTATATCTGAACTTGCTCAGAATGGAACTTCAAGGGCCAAGAGAAAGGCCAGTGGCATACTTGAAAGACTAAACAGGGTAGTTAATATGACCCATACCGCCTAA

Coding sequence (CDS)

ATGGCGAAAACAGGCGTGTTTGATTCTGATCCTAAAGTTATGGGGAAAGCCACGGAGTTGAAGAGGGAGTTACAGAGACTTATCGTTGCTATAATTGATGACGATAATTATTCCACCGACACCATTGATAACGCTAAAGAGATTCTTTCTGCACTTAAGGAGTTGAAACTCAGGAAGAGATCGCGTTCTTCTTCTAATCTTTTTGAGAATCTCATTTGTCCTGAGGAATTTCGTTGTCCGCTTTCTAGAGAACTCATGAGAGATCCTGTCATTGTGTCTACTGGCGAGACATATGATAGACCTTTCATTCACAAATGGTTAAAATCAGGGAACAGGACTTGCCCCAGAACTCAGCAAGTTCTTTCACACACCAATCTCACTCCCAATCACTTGATTCGTGAAATGATATCTCAGTGGTGTACAAGTCGGGGAATTGAGTTGCAAGATCGTGTCCGTGTTCATTATCTTGACGATGATGTGATAACGGAGGCTGATCGAGACCGTTTTCTCACGTTAATAGAGAAAATGTCAATGACAATATCTGAACAGAAAGCAGCTGCTAAGGAGCTTCGAATGTTGACGAAGCGAATGCCCTCATTTCGAGCACTTTTTGGTGAGTCCCTTGATGCCATATCTTTGTTGCTTAGTCCACTTTGTGGAGATAAGTCACAAAGTATTAGTATTCACACGGATCTGCAAGAAGATGTGATCACAACCCTCTTGAATTTGTCGATCCATGACAACAACAAGAAGCTTGTTGCGGAAACACCCAAGGTAATACCTCTTCTAATGGAAGCGTTGAGATCAGGAACCATGGAAACAAGAAGCAATGCAGCTGCAGCCCTATTTACTCTGTCAGCTCTCGACTCAAACAAAGCGATCATCGGGAAAGCTGGTGCGTTGAAACCACTTATCGACCTTTTAGATGAGGGGCATCCTTTATCAATCAAAGATGCTGCTTCAGCAATTTTCAATCTTTGTATAATTCATGAAAACAAAGCAAGAGCTGTGCGAGATGGTGCAGTCCGAGTGCTTCTGAAAAAGATCATGAACCAGATGCATGTTGATGAGTTGTTGGCTATTCTTGCAATGTTATCAAGCCATCAAAAAGCTATCGAAGAAATTGGAGAACTTGGAGCTGTCCCTTTCTTACTCAGAATTATCAGGGAAAGTCCTTGTTCACGTAACAAGGAGAATTGCATTGTGATCATCCATGCTATCTGCTTATACGATCGAACCAAGTGGAAGGATATGAGGGAGGAAGAGAAATGTTATCGAACTATATCTGAACTTGCTCAGAATGGAACTTCAAGGGCCAAGAGAAAGGCCAGTGGCATACTTGAAAGACTAAACAGGGTAGTTAATATGACCCATACCGCCTAA

Protein sequence

MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKRSRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQVLSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTISEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTLLNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLAILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMREEEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA
Homology
BLAST of Lsi05G011400 vs. ExPASy Swiss-Prot
Match: Q9SRT0 (U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 2.9e-156
Identity = 284/464 (61.21%), Postives = 369/464 (79.53%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP  + KA ELKRE+++L++ I D+D+    TID  ++ LSAL+E  +RK 
Sbjct: 1   MAKTGVFDSDPTAIAKAKELKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKM 60

Query: 61  SRSSS-NLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQ 120
           ++SSS  + E + CPEEFRCPLS ELMRDPV++++G+TYD+ FI KWL SGNRTCP+TQQ
Sbjct: 61  AKSSSLEMLETVSCPEEFRCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQ 120

Query: 121 VLSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYL-DDDVITEADRDRFLTLIEKMSM 180
           VL HT LTPN LIREMIS+WC   G+E + +   + + +D+ +T +DR+ F +L+ K+S 
Sbjct: 121 VLPHTALTPNLLIREMISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSS 180

Query: 181 T-ISEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVI 240
           + + +QK+AAKELR+LT++   FRALFGES D I+ L++PL    +        LQEDV+
Sbjct: 181 SNLQDQKSAAKELRLLTRKGTEFRALFGESPDEITRLVNPLLHGSNPD----EKLQEDVV 240

Query: 241 TTLLNLSIH-DNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIG 300
           TTLLN+SIH D+NKKLV E P VIPLL++ALR GT+ TRSNAAAA+FTLSALDSNK +IG
Sbjct: 241 TTLLNISIHDDSNKKLVCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIG 300

Query: 301 KAGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVD 360
           K+G LKPLIDLL+EG+PL+IKD A+AIF LCI HEN++RAVRDGAVRVL KKI N ++VD
Sbjct: 301 KSGILKPLIDLLEEGNPLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVD 360

Query: 361 ELLAILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWK 420
           ELLAILAML +H KA+EE+GELG V +LL+I RES C RNKEN IVI+H IC  DRTKWK
Sbjct: 361 ELLAILAMLVTHWKAVEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWK 420

Query: 421 DMREEEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           +++EEE  + TI++L++ GTSRA+RKA+GIL+RL + +N+THTA
Sbjct: 421 EIKEEENAHGTITKLSREGTSRAQRKANGILDRLRKAMNLTHTA 460

BLAST of Lsi05G011400 vs. ExPASy Swiss-Prot
Match: Q3E9F7 (Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=PUB46 PE=3 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.9e-79
Identity = 182/454 (40.09%), Postives = 282/454 (62.11%), Query Frame = 0

Query: 17  ATELKRELQRLIVAIIDD----DNYSTD-------TIDNAKEILSALKELKLRKRSRSSS 76
           A  L+RELQ+++  I++D    D   T+        ID A  IL+ L++++    S+   
Sbjct: 9   ADTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLRKVE----SKIPE 68

Query: 77  NLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQVLSHTN 136
           +    +  P+EF C LS  +M +PVI+++G+TY++ +I +WLK   RTCP+T+QVLSH  
Sbjct: 69  SDISPVEVPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWLKH-ERTCPKTKQVLSHRL 128

Query: 137 LTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKM--SMTISEQ 196
             PNHLI ++I+QWC     + Q         D+++ E        L++++  S ++++Q
Sbjct: 129 WIPNHLISDLITQWCLVNKYDHQKP------SDELVAELFTSDIEALLQRVSSSSSVADQ 188

Query: 197 KAAAKELRMLTKRMPSFRALFGESL-DAISLLLSPLCGDKSQSISIHTDLQEDVITTLLN 256
             AAKELR  TK+ P+ R  F   + D+I+ LLSPL     +++    +LQE+++T L N
Sbjct: 189 IEAAKELRHQTKKFPNVRVFFVAGIHDSITRLLSPL-STLDEAVDSSLELQENIVTALFN 248

Query: 257 LSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGALK 316
           LSI ++NK ++AE   VIPLL ++L+ GT ETR NAAA L +LSA+DSNK IIG + A+K
Sbjct: 249 LSILESNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVK 308

Query: 317 PLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 376
            LIDL++EG  L+ K+A S +FNLCI+ ENK + V  G +    KKI    +VDELL++L
Sbjct: 309 ALIDLIEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKIKAGSNVDELLSLL 368

Query: 377 AMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAIC--LYDRTKWKDMRE 436
           A++S+H +A+EE+ +LG +  L  I+R+       EN +VI+  +     DR++ K + E
Sbjct: 369 ALISTHNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGE 428

Query: 437 EEKCYRTISELAQNGTSRAKRKASGILERLNRVV 455
           EE  + T ++LA+ G+ RA RKA GIL+ + R V
Sbjct: 429 EENQHGTFTKLAKQGSVRAARKAQGILQWIKRFV 450

BLAST of Lsi05G011400 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 8.1e-74
Identity = 178/446 (39.91%), Postives = 267/446 (59.87%), Query Frame = 0

Query: 19  ELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRK-------RSRSSSNLFENL 78
           ++K E   L   +I         +D    +L  LK+  + +        +RS+S    + 
Sbjct: 167 DMKNESIALHNMVISTAGEPDGCVDQMSSLLKKLKDCVVTEDHANDALTTRSASIKHRSP 226

Query: 79  ICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQVLSHTNLTPNHL 138
           I P+EFRCP+S ELM+DPVIVS+G+TY+R  I KWL SG++TCP+TQQ LSHT+LTPN +
Sbjct: 227 IIPDEFRCPISLELMQDPVIVSSGQTYERSCIQKWLDSGHKTCPKTQQPLSHTSLTPNFV 286

Query: 139 IREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDR--FLTLIEKM-SMTISEQKAAAK 198
           ++ +ISQWC + GIEL  + + +  D      +D D    ++L+ ++ S    EQ+AAA 
Sbjct: 287 LKSLISQWCEANGIEL-PKNKQNSRDKKAAKSSDYDHAGLVSLMNRLRSGNQDEQRAAAG 346

Query: 199 ELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTLLNLSIHDN 258
           E+R+L KR  + R    E+  AI LL++ L     ++       QE  +T LLNLSIH+N
Sbjct: 347 EIRLLAKRNVNNRICIAEA-GAIPLLVNLLSSSDPRT-------QEHAVTALLNLSIHEN 406

Query: 259 NKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGALKPLIDLL 318
           NK  + ++   IP ++E L++G+METR NAAA LF+LS +D NK  IG AGA+ PLI+LL
Sbjct: 407 NKASIVDS-HAIPKIVEVLKTGSMETRENAAATLFSLSVVDENKVTIGAAGAIPPLINLL 466

Query: 319 DEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQM--HVDELLAILAMLS 378
            +G P   KDAA+AIFNLCI   NK RAV+ G V  L+  +++     +DE L++L++L+
Sbjct: 467 CDGSPRGKKDAATAIFNLCIYQGNKVRAVKAGIVIHLMNFLVDPTGGMIDEALSLLSILA 526

Query: 379 SHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMREEEKCYR 438
            + +    I     +P L+ +I+ +   RN+EN   I+  +C  D T+     +      
Sbjct: 527 GNPEGKIVIARSEPIPPLVEVIK-TGSPRNRENAAAILWLLCSAD-TEQTLAAKAAGVED 586

Query: 439 TISELAQNGTSRAKRKASGILERLNR 453
            + EL++ GT RAKRKAS ILE +++
Sbjct: 587 ALKELSETGTDRAKRKASSILELMHQ 600

BLAST of Lsi05G011400 vs. ExPASy Swiss-Prot
Match: Q8VZ40 (U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.1e-73
Identity = 183/476 (38.45%), Postives = 267/476 (56.09%), Query Frame = 0

Query: 6   VFDSDPKVMGKAT---------ELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELK 65
           V D DP ++ + +         ELK+E   +    +  D    D  +    +L  L +  
Sbjct: 165 VMDPDPIILKRLSQELQLTTIDELKKESHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFV 224

Query: 66  LRKRSRSSSNLFENL-------ICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKS 125
             + S    +    +       + PE FRCP+S ELM+DPVIVSTG+TY+R  I KWL +
Sbjct: 225 TMESSDPDPSTGSRIVSRHRSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDA 284

Query: 126 GNRTCPRTQQVLSHTNLTPNHLIREMISQWCTSRGIEL---QDRVRVHYLDDDVITEADR 185
           G++TCP++Q+ L H  LTPN++++ +I+ WC S GIEL   Q   R   +     ++ DR
Sbjct: 285 GHKTCPKSQETLLHAGLTPNYVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDR 344

Query: 186 DRFLTLIEKMSM-TISEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS 245
              L+L+EK++  T  +Q+AAA ELR+L KR    R    E+  AI LL+  L     ++
Sbjct: 345 TFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEA-GAIPLLVELLSSPDPRT 404

Query: 246 ISIHTDLQEDVITTLLNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTL 305
                  QE  +T LLNLSI++ NK  + +   +   ++E L++G+ME R NAAA LF+L
Sbjct: 405 -------QEHSVTALLNLSINEGNKGAIVDAGAITD-IVEVLKNGSMEARENAAATLFSL 464

Query: 306 SALDSNKAIIGKAGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVL 365
           S +D NK  IG AGA++ LI LL+EG     KDAA+AIFNLCI   NK+RAV+ G V  L
Sbjct: 465 SVIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPL 524

Query: 366 --LKKIMNQMHVDELLAILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVI 425
             L K      VDE LAILA+LS++Q+    I E  ++P L+ IIR +   RN+EN   I
Sbjct: 525 TRLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIR-TGSPRNRENAAAI 584

Query: 426 IHAICLYDRTKWKDMREEEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHT 460
           +  +C+ +  +    RE       + EL +NGT RAKRKA+ +LE + +   +  T
Sbjct: 585 LWYLCIGNIERLNVAREVGADV-ALKELTENGTDRAKRKAASLLELIQQTEGVAVT 629

BLAST of Lsi05G011400 vs. ExPASy Swiss-Prot
Match: Q9ZV31 (U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 7.8e-69
Identity = 168/414 (40.58%), Postives = 246/414 (59.42%), Query Frame = 0

Query: 52  LKELKLRKRS---RSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLK 111
           L +  LR +S   +S  +  + LI PEEFRCP+S ELM DPVIVS+G+TY+R  I KWL+
Sbjct: 232 LDDAPLRLKSSLPKSRDDDRDMLIPPEEFRCPISLELMTDPVIVSSGQTYERECIKKWLE 291

Query: 112 SGNRTCPRTQQVLSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEA---- 171
            G+ TCP+TQ+ L+   +TPN+++R +I+QWC S GIE   R  +        + +    
Sbjct: 292 GGHLTCPKTQETLTSDIMTPNYVLRSLIAQWCESNGIEPPKRPNISQPSSKASSSSSAPD 351

Query: 172 -DRDRFLTLIEKM-SMTISEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDK 231
            + ++   L+ K+ S    ++++AA E+R+L K+    R     S  AI LL++ L    
Sbjct: 352 DEHNKIEELLLKLTSQQPEDRRSAAGEIRLLAKQNNHNRVAIAAS-GAIPLLVNLL---- 411

Query: 232 SQSISIHTDLQEDVITTLLNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAAL 291
             +IS  +  QE  +T++LNLSI   NK  +  +   +P ++  L+ G+ME R NAAA L
Sbjct: 412 --TISNDSRTQEHAVTSILNLSICQENKGKIVYSSGAVPGIVHVLQKGSMEARENAAATL 471

Query: 292 FTLSALDSNKAIIGKAGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAV 351
           F+LS +D NK  IG AGA+ PL+ LL EG     KDAA+A+FNLCI   NK +AVR G V
Sbjct: 472 FSLSVIDENKVTIGAAGAIPPLVTLLSEGSQRGKKDAATALFNLCIFQGNKGKAVRAGLV 531

Query: 352 RVLLKKIMNQMH--VDELLAILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENC 411
            VL++ +       VDE L+ILA+LSSH     E+G   AVP L+  IR S   RNKEN 
Sbjct: 532 PVLMRLLTEPESGMVDESLSILAILSSHPDGKSEVGAADAVPVLVDFIR-SGSPRNKENS 591

Query: 412 IVIIHAICLYDRTKWKDMREEEK--CYRTISELAQNGTSRAKRKASGILERLNR 453
             ++  +C +++   + + E +K      + E+A+NGT R KRKA+ +L R +R
Sbjct: 592 AAVLVHLCSWNQ---QHLIEAQKLGIMDLLIEMAENGTDRGKRKAAQLLNRFSR 634

BLAST of Lsi05G011400 vs. ExPASy TrEMBL
Match: A0A6J1HUJ6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466836 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 4.5e-237
Identity = 430/460 (93.48%), Postives = 444/460 (96.52%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP VMGKATELKRELQRLI AIIDDDN STDTID+AKEI+SALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQV 120
           SR SSN +ENLICP+EFRCPLSRELMRDPV+VSTGETYDRPFIHKWL+SGNRTCPRTQQV
Sbjct: 61  SR-SSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180
           LSHTNLTPNHLIRE+ISQWCTSRGIELQDRVRVH LDDDVITEADRDRFLTLIE MSMTI
Sbjct: 121 LSHTNLTPNHLIREIISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTL 240
           SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS S+H DLQEDVITTL
Sbjct: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS-SVHADLQEDVITTL 240

Query: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300
           LNLSIHDNNKKLVAETP VIPLL EALRSGTMETRSNAAAALFTLSALDSNKA+IGK+GA
Sbjct: 241 LNLSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGA 300

Query: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360
           LKPLIDLLDEGHPLSIKDAASAIFNLCI+HENKARAVRDGAVRVLLKKIMNQMHVDELLA
Sbjct: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360

Query: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMRE 420
           ILAMLSSHQKA+EEIGELGAVP L RIIRES CSRNKENCIVIIHAICLYDRTKWKDM+E
Sbjct: 361 ILAMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKE 420

Query: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           EEK YRTISELA+NGTSRAKRKASGILERLNR VNMTHTA
Sbjct: 421 EEKWYRTISELAKNGTSRAKRKASGILERLNRAVNMTHTA 458

BLAST of Lsi05G011400 vs. ExPASy TrEMBL
Match: A0A6J1DYS9 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111025869 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 4.5e-237
Identity = 430/460 (93.48%), Postives = 446/460 (96.96%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP VMGKATELK+ELQRLI+AIIDDDNYSTDTID+AKEIL ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60

Query: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQV 120
           SR SSNL+ENLICP++FRCPLSRELMRDPV+VSTGETYDRPFIHKWLK+GNRTCPRTQQV
Sbjct: 61  SR-SSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180
           LSHTNLTPNHLIREMISQWC SRGIELQD  RVHYLDDD ITEADRD+FLTLIEKMSMTI
Sbjct: 121 LSHTNLTPNHLIREMISQWCKSRGIELQD--RVHYLDDDTITEADRDQFLTLIEKMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTL 240
           SEQKAAAKELRMLTKRMPSFRALFGESLDAIS LLSPLCGD SQS SIHTDLQEDVITTL
Sbjct: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQS-SIHTDLQEDVITTL 240

Query: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300
           LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKA+IGK+GA
Sbjct: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGA 300

Query: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360
           L+PLIDLLDEGHPL+IKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIM QMHVDELLA
Sbjct: 301 LEPLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLA 360

Query: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMRE 420
           ILAMLS+H KAIEE+GELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKD+RE
Sbjct: 361 ILAMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIRE 420

Query: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           EEK YRTISELAQNGTSRAKRKASGILERLNR VNMTHTA
Sbjct: 421 EEKSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 456

BLAST of Lsi05G011400 vs. ExPASy TrEMBL
Match: A0A6J1HKK5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111463870 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 4.5e-237
Identity = 430/460 (93.48%), Postives = 443/460 (96.30%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP VMGKATELKRELQRLI AIIDDDN STDTID+AKEI+SALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQV 120
           SR SSN +ENLICP+EFRCPLSRELMRDPV+VSTGETYDRPFIHKWL+SGNRTCPRTQQV
Sbjct: 61  SR-SSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180
           LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVH LDDDVITEADRDRFLTLIE MSMTI
Sbjct: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTL 240
            EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS S+H DLQEDVITTL
Sbjct: 181 PEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS-SVHADLQEDVITTL 240

Query: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300
           LNLSIHDNNKKLVAETP VIPLL EALRSGTMETRSNAAAALFTLSALDSNKA+IGK+GA
Sbjct: 241 LNLSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGA 300

Query: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360
           LKPLIDLLDEGHPLS+KDAASAIFNLCI+HENKARAVRDGAVRVLLKKIMNQMHVDELLA
Sbjct: 301 LKPLIDLLDEGHPLSMKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360

Query: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMRE 420
           ILAMLSSHQKA+EEIGELGAVP L RIIRES CSRNKENCIVIIHAICLYDRTKWKDM+E
Sbjct: 361 ILAMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKE 420

Query: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           EEK YRTISELAQNGTSRAKRKASGILERLNR VNMTHTA
Sbjct: 421 EEKWYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 458

BLAST of Lsi05G011400 vs. ExPASy TrEMBL
Match: A0A5D3DRP9 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1185G00250 PE=4 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.9e-235
Identity = 425/461 (92.19%), Postives = 443/461 (96.10%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP V+GKATELK+ELQ LI+AIIDDDNYSTDTIDNAK+ILSALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVIGKATELKKELQTLILAIIDDDNYSTDTIDNAKDILSALKELKLRKR 60

Query: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQV 120
           SRSSSNLF NLI P+EFRCPLSRELMRDPVI+STGETYDRPFI KWL SGNRTCPRTQQV
Sbjct: 61  SRSSSNLFHNLISPQEFRCPLSRELMRDPVILSTGETYDRPFIQKWLNSGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180
           LSHT+LTPNHLIREMISQWC +RGIELQDRVRVHYLDDDVITEADRDRFL L+EKMSMTI
Sbjct: 121 LSHTDLTPNHLIREMISQWCATRGIELQDRVRVHYLDDDVITEADRDRFLMLLEKMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSIS-IHTDLQEDVITT 240
           SEQK AAKELRMLTKRMPSFRALFGESLDAISLLLSPL GDKSQS S IHTDLQEDVITT
Sbjct: 181 SEQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLSGDKSQSNSNIHTDLQEDVITT 240

Query: 241 LLNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAG 300
           LLNLSIHDNNKKLVAETP  IPLLMEALRSGTMETRSNAAAALFTLSALD NKAIIGKAG
Sbjct: 241 LLNLSIHDNNKKLVAETPYAIPLLMEALRSGTMETRSNAAAALFTLSALDPNKAIIGKAG 300

Query: 301 ALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELL 360
           ALKPLI+LLD GHPLSIKDAASAIFNLCI+HENKARAVRDGAVRVLLKKIM+QMHVDELL
Sbjct: 301 ALKPLIELLDLGHPLSIKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMSQMHVDELL 360

Query: 361 AILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMR 420
           AILAMLS HQ+AIEEIGELGAVPFLLRIIRES CSRNKENCIVIIH++CLYDRTKWK+MR
Sbjct: 361 AILAMLSCHQRAIEEIGELGAVPFLLRIIRESSCSRNKENCIVIIHSVCLYDRTKWKEMR 420

Query: 421 EEEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           EEEKCYRTISELAQNGTSRAKRKASG+LER+NRVVNMTHTA
Sbjct: 421 EEEKCYRTISELAQNGTSRAKRKASGVLERINRVVNMTHTA 461

BLAST of Lsi05G011400 vs. ExPASy TrEMBL
Match: A0A1S3CDM9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499224 PE=4 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.9e-235
Identity = 425/461 (92.19%), Postives = 443/461 (96.10%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP V+GKATELK+ELQ LI+AIIDDDNYSTDTIDNAK+ILSALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVIGKATELKKELQTLILAIIDDDNYSTDTIDNAKDILSALKELKLRKR 60

Query: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQV 120
           SRSSSNLF NLI P+EFRCPLSRELMRDPVI+STGETYDRPFI KWL SGNRTCPRTQQV
Sbjct: 61  SRSSSNLFHNLISPQEFRCPLSRELMRDPVILSTGETYDRPFIQKWLNSGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180
           LSHT+LTPNHLIREMISQWC +RGIELQDRVRVHYLDDDVITEADRDRFL L+EKMSMTI
Sbjct: 121 LSHTDLTPNHLIREMISQWCATRGIELQDRVRVHYLDDDVITEADRDRFLMLLEKMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSIS-IHTDLQEDVITT 240
           SEQK AAKELRMLTKRMPSFRALFGESLDAISLLLSPL GDKSQS S IHTDLQEDVITT
Sbjct: 181 SEQKEAAKELRMLTKRMPSFRALFGESLDAISLLLSPLSGDKSQSNSNIHTDLQEDVITT 240

Query: 241 LLNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAG 300
           LLNLSIHDNNKKLVAETP  IPLLMEALRSGTMETRSNAAAALFTLSALD NKAIIGKAG
Sbjct: 241 LLNLSIHDNNKKLVAETPYAIPLLMEALRSGTMETRSNAAAALFTLSALDPNKAIIGKAG 300

Query: 301 ALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELL 360
           ALKPLI+LLD GHPLSIKDAASAIFNLCI+HENKARAVRDGAVRVLLKKIM+QMHVDELL
Sbjct: 301 ALKPLIELLDLGHPLSIKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMSQMHVDELL 360

Query: 361 AILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMR 420
           AILAMLS HQ+AIEEIGELGAVPFLLRIIRES CSRNKENCIVIIH++CLYDRTKWK+MR
Sbjct: 361 AILAMLSCHQRAIEEIGELGAVPFLLRIIRESSCSRNKENCIVIIHSVCLYDRTKWKEMR 420

Query: 421 EEEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           EEEKCYRTISELAQNGTSRAKRKASG+LER+NRVVNMTHTA
Sbjct: 421 EEEKCYRTISELAQNGTSRAKRKASGVLERINRVVNMTHTA 461

BLAST of Lsi05G011400 vs. NCBI nr
Match: XP_038888241.1 (U-box domain-containing protein 9 [Benincasa hispida])

HSP 1 Score: 871.3 bits (2250), Expect = 3.7e-249
Identity = 451/460 (98.04%), Postives = 452/460 (98.26%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP VMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60

Query: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQV 120
           SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYD+PFI KWLKSGNRTCPRTQQV
Sbjct: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDKPFIQKWLKSGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180
           LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI
Sbjct: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTL 240
           SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS SIHTDLQEDVITTL
Sbjct: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSSSIHTDLQEDVITTL 240

Query: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300
           LNLSIHDNNKKLV ETP VIPLLMEAL SGTMETRSNAAAALFTLSALDSNKAIIGKAGA
Sbjct: 241 LNLSIHDNNKKLVIETPNVIPLLMEALGSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300

Query: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360
           LKPLI LLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA
Sbjct: 301 LKPLIKLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360

Query: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMRE 420
           ILAMLSSHQKAIEEIGELGAVPFLLRIIRES CSRNKENCIVIIHAICLYDRTKWKDMRE
Sbjct: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMRE 420

Query: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA
Sbjct: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 460

BLAST of Lsi05G011400 vs. NCBI nr
Match: XP_022967253.1 (U-box domain-containing protein 9-like [Cucurbita maxima])

HSP 1 Score: 830.1 bits (2143), Expect = 9.4e-237
Identity = 430/460 (93.48%), Postives = 444/460 (96.52%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP VMGKATELKRELQRLI AIIDDDN STDTID+AKEI+SALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQV 120
           SR SSN +ENLICP+EFRCPLSRELMRDPV+VSTGETYDRPFIHKWL+SGNRTCPRTQQV
Sbjct: 61  SR-SSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180
           LSHTNLTPNHLIRE+ISQWCTSRGIELQDRVRVH LDDDVITEADRDRFLTLIE MSMTI
Sbjct: 121 LSHTNLTPNHLIREIISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTL 240
           SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS S+H DLQEDVITTL
Sbjct: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS-SVHADLQEDVITTL 240

Query: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300
           LNLSIHDNNKKLVAETP VIPLL EALRSGTMETRSNAAAALFTLSALDSNKA+IGK+GA
Sbjct: 241 LNLSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGA 300

Query: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360
           LKPLIDLLDEGHPLSIKDAASAIFNLCI+HENKARAVRDGAVRVLLKKIMNQMHVDELLA
Sbjct: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360

Query: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMRE 420
           ILAMLSSHQKA+EEIGELGAVP L RIIRES CSRNKENCIVIIHAICLYDRTKWKDM+E
Sbjct: 361 ILAMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKE 420

Query: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           EEK YRTISELA+NGTSRAKRKASGILERLNR VNMTHTA
Sbjct: 421 EEKWYRTISELAKNGTSRAKRKASGILERLNRAVNMTHTA 458

BLAST of Lsi05G011400 vs. NCBI nr
Match: XP_022963574.1 (U-box domain-containing protein 9-like [Cucurbita moschata])

HSP 1 Score: 830.1 bits (2143), Expect = 9.4e-237
Identity = 430/460 (93.48%), Postives = 443/460 (96.30%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP VMGKATELKRELQRLI AIIDDDN STDTID+AKEI+SALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQV 120
           SR SSN +ENLICP+EFRCPLSRELMRDPV+VSTGETYDRPFIHKWL+SGNRTCPRTQQV
Sbjct: 61  SR-SSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180
           LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVH LDDDVITEADRDRFLTLIE MSMTI
Sbjct: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHCLDDDVITEADRDRFLTLIENMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTL 240
            EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS S+H DLQEDVITTL
Sbjct: 181 PEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS-SVHADLQEDVITTL 240

Query: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300
           LNLSIHDNNKKLVAETP VIPLL EALRSGTMETRSNAAAALFTLSALDSNKA+IGK+GA
Sbjct: 241 LNLSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGA 300

Query: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360
           LKPLIDLLDEGHPLS+KDAASAIFNLCI+HENKARAVRDGAVRVLLKKIMNQMHVDELLA
Sbjct: 301 LKPLIDLLDEGHPLSMKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360

Query: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMRE 420
           ILAMLSSHQKA+EEIGELGAVP L RIIRES CSRNKENCIVIIHAICLYDRTKWKDM+E
Sbjct: 361 ILAMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKE 420

Query: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           EEK YRTISELAQNGTSRAKRKASGILERLNR VNMTHTA
Sbjct: 421 EEKWYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 458

BLAST of Lsi05G011400 vs. NCBI nr
Match: XP_022159455.1 (U-box domain-containing protein 9 [Momordica charantia])

HSP 1 Score: 830.1 bits (2143), Expect = 9.4e-237
Identity = 430/460 (93.48%), Postives = 446/460 (96.96%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP VMGKATELK+ELQRLI+AIIDDDNYSTDTID+AKEIL ALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKKELQRLILAIIDDDNYSTDTIDHAKEILCALKELKLRKR 60

Query: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQV 120
           SR SSNL+ENLICP++FRCPLSRELMRDPV+VSTGETYDRPFIHKWLK+GNRTCPRTQQV
Sbjct: 61  SR-SSNLYENLICPDQFRCPLSRELMRDPVVVSTGETYDRPFIHKWLKAGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180
           LSHTNLTPNHLIREMISQWC SRGIELQD  RVHYLDDD ITEADRD+FLTLIEKMSMTI
Sbjct: 121 LSHTNLTPNHLIREMISQWCKSRGIELQD--RVHYLDDDTITEADRDQFLTLIEKMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTL 240
           SEQKAAAKELRMLTKRMPSFRALFGESLDAIS LLSPLCGD SQS SIHTDLQEDVITTL
Sbjct: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISSLLSPLCGDNSQS-SIHTDLQEDVITTL 240

Query: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300
           LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKA+IGK+GA
Sbjct: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGA 300

Query: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360
           L+PLIDLLDEGHPL+IKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIM QMHVDELLA
Sbjct: 301 LEPLIDLLDEGHPLAIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMKQMHVDELLA 360

Query: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMRE 420
           ILAMLS+H KAIEE+GELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKD+RE
Sbjct: 361 ILAMLSNHPKAIEEMGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDIRE 420

Query: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           EEK YRTISELAQNGTSRAKRKASGILERLNR VNMTHTA
Sbjct: 421 EEKSYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 456

BLAST of Lsi05G011400 vs. NCBI nr
Match: XP_023554208.1 (U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 828.9 bits (2140), Expect = 2.1e-236
Identity = 430/460 (93.48%), Postives = 443/460 (96.30%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP VMGKATELKRELQRLI AIIDDDN STDTID+AKEI+SALKELKLRKR
Sbjct: 1   MAKTGVFDSDPTVMGKATELKRELQRLIDAIIDDDNCSTDTIDHAKEIISALKELKLRKR 60

Query: 61  SRSSSNLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQV 120
           SR SSN +ENLICP+EFRCPLSRELMRDPV+VSTGETYDRPFIHKWL+SGNRTCPRTQQV
Sbjct: 61  SR-SSNRYENLICPDEFRCPLSRELMRDPVVVSTGETYDRPFIHKWLRSGNRTCPRTQQV 120

Query: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKMSMTI 180
           LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVH L+DDVITEADRDRFLTLIE MSMTI
Sbjct: 121 LSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHCLEDDVITEADRDRFLTLIENMSMTI 180

Query: 181 SEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTL 240
            EQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS SIH DLQEDVITTL
Sbjct: 181 PEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS-SIHADLQEDVITTL 240

Query: 241 LNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGA 300
           LNLSIHDNNKKLVAETP VIPLL EALRSGTMETRSNAAAALFTLSALDSNKA+IGK+GA
Sbjct: 241 LNLSIHDNNKKLVAETPNVIPLLKEALRSGTMETRSNAAAALFTLSALDSNKALIGKSGA 300

Query: 301 LKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360
           LKPLIDLLDEGHPLS+KDAASAIFNLCI+HENKARAVRDGAVRVLLKKIMNQMHVDELLA
Sbjct: 301 LKPLIDLLDEGHPLSMKDAASAIFNLCILHENKARAVRDGAVRVLLKKIMNQMHVDELLA 360

Query: 361 ILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMRE 420
           ILAMLSSHQKA+EEIGELGAVP L RIIRES CSRNKENCIVIIHAICLYDRTKWKDM+E
Sbjct: 361 ILAMLSSHQKAVEEIGELGAVPSLFRIIRESSCSRNKENCIVIIHAICLYDRTKWKDMKE 420

Query: 421 EEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           EEK YRTISELAQNGTSRAKRKASGILERLNR VNMTHTA
Sbjct: 421 EEKWYRTISELAQNGTSRAKRKASGILERLNRAVNMTHTA 458

BLAST of Lsi05G011400 vs. TAIR 10
Match: AT3G07360.1 (plant U-box 9 )

HSP 1 Score: 553.1 bits (1424), Expect = 2.1e-157
Identity = 284/464 (61.21%), Postives = 369/464 (79.53%), Query Frame = 0

Query: 1   MAKTGVFDSDPKVMGKATELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELKLRKR 60
           MAKTGVFDSDP  + KA ELKRE+++L++ I D+D+    TID  ++ LSAL+E  +RK 
Sbjct: 1   MAKTGVFDSDPTAIAKAKELKREMKKLLIKIDDEDDLGVQTIDQLQDALSALREATMRKM 60

Query: 61  SRSSS-NLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQ 120
           ++SSS  + E + CPEEFRCPLS ELMRDPV++++G+TYD+ FI KWL SGNRTCP+TQQ
Sbjct: 61  AKSSSLEMLETVSCPEEFRCPLSNELMRDPVVLASGQTYDKLFIQKWLSSGNRTCPKTQQ 120

Query: 121 VLSHTNLTPNHLIREMISQWCTSRGIELQDRVRVHYL-DDDVITEADRDRFLTLIEKMSM 180
           VL HT LTPN LIREMIS+WC   G+E + +   + + +D+ +T +DR+ F +L+ K+S 
Sbjct: 121 VLPHTALTPNLLIREMISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSS 180

Query: 181 T-ISEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVI 240
           + + +QK+AAKELR+LT++   FRALFGES D I+ L++PL    +        LQEDV+
Sbjct: 181 SNLQDQKSAAKELRLLTRKGTEFRALFGESPDEITRLVNPLLHGSNPD----EKLQEDVV 240

Query: 241 TTLLNLSIH-DNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIG 300
           TTLLN+SIH D+NKKLV E P VIPLL++ALR GT+ TRSNAAAA+FTLSALDSNK +IG
Sbjct: 241 TTLLNISIHDDSNKKLVCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIG 300

Query: 301 KAGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVD 360
           K+G LKPLIDLL+EG+PL+IKD A+AIF LCI HEN++RAVRDGAVRVL KKI N ++VD
Sbjct: 301 KSGILKPLIDLLEEGNPLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVD 360

Query: 361 ELLAILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWK 420
           ELLAILAML +H KA+EE+GELG V +LL+I RES C RNKEN IVI+H IC  DRTKWK
Sbjct: 361 ELLAILAMLVTHWKAVEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWK 420

Query: 421 DMREEEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHTA 461
           +++EEE  + TI++L++ GTSRA+RKA+GIL+RL + +N+THTA
Sbjct: 421 EIKEEENAHGTITKLSREGTSRAQRKANGILDRLRKAMNLTHTA 460

BLAST of Lsi05G011400 vs. TAIR 10
Match: AT3G07360.2 (plant U-box 9 )

HSP 1 Score: 379.4 bits (973), Expect = 4.1e-105
Identity = 200/329 (60.79%), Postives = 262/329 (79.64%), Query Frame = 0

Query: 135 MISQWCTSRGIELQDRVRVHYL-DDDVITEADRDRFLTLIEKMSMT-ISEQKAAAKELRM 194
           MIS+WC   G+E + +   + + +D+ +T +DR+ F +L+ K+S + + +QK+AAKELR+
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 195 LTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTLLNLSIH-DNNKK 254
           LT++   FRALFGES D I+ L++PL    +        LQEDV+TTLLN+SIH D+NKK
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPLLHGSNPD----EKLQEDVVTTLLNISIHDDSNKK 120

Query: 255 LVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGALKPLIDLLDEG 314
           LV E P VIPLL++ALR GT+ TRSNAAAA+FTLSALDSNK +IGK+G LKPLIDLL+EG
Sbjct: 121 LVCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEG 180

Query: 315 HPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLAILAMLSSHQKA 374
           +PL+IKD A+AIF LCI HEN++RAVRDGAVRVL KKI N ++VDELLAILAML +H KA
Sbjct: 181 NPLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKA 240

Query: 375 IEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMREEEKCYRTISEL 434
           +EE+GELG V +LL+I RES C RNKEN IVI+H IC  DRTKWK+++EEE  + TI++L
Sbjct: 241 VEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKL 300

Query: 435 AQNGTSRAKRKASGILERLNRVVNMTHTA 461
           ++ GTSRA+RKA+GIL+RL + +N+THTA
Sbjct: 301 SREGTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of Lsi05G011400 vs. TAIR 10
Match: AT3G07360.3 (plant U-box 9 )

HSP 1 Score: 379.4 bits (973), Expect = 4.1e-105
Identity = 200/329 (60.79%), Postives = 262/329 (79.64%), Query Frame = 0

Query: 135 MISQWCTSRGIELQDRVRVHYL-DDDVITEADRDRFLTLIEKMSMT-ISEQKAAAKELRM 194
           MIS+WC   G+E + +   + + +D+ +T +DR+ F +L+ K+S + + +QK+AAKELR+
Sbjct: 1   MISKWCKKNGLETKSQYHPNLVNEDETVTRSDREIFNSLLCKVSSSNLQDQKSAAKELRL 60

Query: 195 LTKRMPSFRALFGESLDAISLLLSPLCGDKSQSISIHTDLQEDVITTLLNLSIH-DNNKK 254
           LT++   FRALFGES D I+ L++PL    +        LQEDV+TTLLN+SIH D+NKK
Sbjct: 61  LTRKGTEFRALFGESPDEITRLVNPLLHGSNPD----EKLQEDVVTTLLNISIHDDSNKK 120

Query: 255 LVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGALKPLIDLLDEG 314
           LV E P VIPLL++ALR GT+ TRSNAAAA+FTLSALDSNK +IGK+G LKPLIDLL+EG
Sbjct: 121 LVCENPNVIPLLIDALRRGTVATRSNAAAAIFTLSALDSNKVLIGKSGILKPLIDLLEEG 180

Query: 315 HPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLAILAMLSSHQKA 374
           +PL+IKD A+AIF LCI HEN++RAVRDGAVRVL KKI N ++VDELLAILAML +H KA
Sbjct: 181 NPLAIKDVAAAIFTLCIAHENRSRAVRDGAVRVLGKKISNGLYVDELLAILAMLVTHWKA 240

Query: 375 IEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAICLYDRTKWKDMREEEKCYRTISEL 434
           +EE+GELG V +LL+I RES C RNKEN IVI+H IC  DRTKWK+++EEE  + TI++L
Sbjct: 241 VEELGELGGVSWLLKITRESECKRNKENAIVILHTICFSDRTKWKEIKEEENAHGTITKL 300

Query: 435 AQNGTSRAKRKASGILERLNRVVNMTHTA 461
           ++ GTSRA+RKA+GIL+RL + +N+THTA
Sbjct: 301 SREGTSRAQRKANGILDRLRKAMNLTHTA 325

BLAST of Lsi05G011400 vs. TAIR 10
Match: AT5G18320.1 (ARM repeat superfamily protein )

HSP 1 Score: 297.4 bits (760), Expect = 2.0e-80
Identity = 182/454 (40.09%), Postives = 282/454 (62.11%), Query Frame = 0

Query: 17  ATELKRELQRLIVAIIDD----DNYSTD-------TIDNAKEILSALKELKLRKRSRSSS 76
           A  L+RELQ+++  I++D    D   T+        ID A  IL+ L++++    S+   
Sbjct: 9   ADTLRRELQKVLTEILNDGGGNDRDETEAFSGVVKAIDEAVRILTCLRKVE----SKIPE 68

Query: 77  NLFENLICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKSGNRTCPRTQQVLSHTN 136
           +    +  P+EF C LS  +M +PVI+++G+TY++ +I +WLK   RTCP+T+QVLSH  
Sbjct: 69  SDISPVEVPKEFICTLSNTIMIEPVIIASGQTYEKRYITEWLKH-ERTCPKTKQVLSHRL 128

Query: 137 LTPNHLIREMISQWCTSRGIELQDRVRVHYLDDDVITEADRDRFLTLIEKM--SMTISEQ 196
             PNHLI ++I+QWC     + Q         D+++ E        L++++  S ++++Q
Sbjct: 129 WIPNHLISDLITQWCLVNKYDHQKP------SDELVAELFTSDIEALLQRVSSSSSVADQ 188

Query: 197 KAAAKELRMLTKRMPSFRALFGESL-DAISLLLSPLCGDKSQSISIHTDLQEDVITTLLN 256
             AAKELR  TK+ P+ R  F   + D+I+ LLSPL     +++    +LQE+++T L N
Sbjct: 189 IEAAKELRHQTKKFPNVRVFFVAGIHDSITRLLSPL-STLDEAVDSSLELQENIVTALFN 248

Query: 257 LSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTLSALDSNKAIIGKAGALK 316
           LSI ++NK ++AE   VIPLL ++L+ GT ETR NAAA L +LSA+DSNK IIG + A+K
Sbjct: 249 LSILESNKTVIAENCLVIPLLTKSLKQGTDETRRNAAATLSSLSAIDSNKIIIGNSEAVK 308

Query: 317 PLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVLLKKIMNQMHVDELLAIL 376
            LIDL++EG  L+ K+A S +FNLCI+ ENK + V  G +    KKI    +VDELL++L
Sbjct: 309 ALIDLIEEGDLLATKEATSTVFNLCIVLENKGKVVSAGLIHAATKKIKAGSNVDELLSLL 368

Query: 377 AMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVIIHAIC--LYDRTKWKDMRE 436
           A++S+H +A+EE+ +LG +  L  I+R+       EN +VI+  +     DR++ K + E
Sbjct: 369 ALISTHNRAVEEMDKLGFIYDLFSILRKPSSLLTGENAVVIVFNMYDRNRDRSRLKVVGE 428

Query: 437 EEKCYRTISELAQNGTSRAKRKASGILERLNRVV 455
           EE  + T ++LA+ G+ RA RKA GIL+ + R V
Sbjct: 429 EENQHGTFTKLAKQGSVRAARKAQGILQWIKRFV 450

BLAST of Lsi05G011400 vs. TAIR 10
Match: AT3G54850.1 (plant U-box 14 )

HSP 1 Score: 278.9 bits (712), Expect = 7.5e-75
Identity = 183/476 (38.45%), Postives = 267/476 (56.09%), Query Frame = 0

Query: 6   VFDSDPKVMGKAT---------ELKRELQRLIVAIIDDDNYSTDTIDNAKEILSALKELK 65
           V D DP ++ + +         ELK+E   +    +  D    D  +    +L  L +  
Sbjct: 165 VMDPDPIILKRLSQELQLTTIDELKKESHAIHEYFLSYDGDPDDCFERMSSLLKNLVDFV 224

Query: 66  LRKRSRSSSNLFENL-------ICPEEFRCPLSRELMRDPVIVSTGETYDRPFIHKWLKS 125
             + S    +    +       + PE FRCP+S ELM+DPVIVSTG+TY+R  I KWL +
Sbjct: 225 TMESSDPDPSTGSRIVSRHRSPVIPEYFRCPISLELMKDPVIVSTGQTYERSSIQKWLDA 284

Query: 126 GNRTCPRTQQVLSHTNLTPNHLIREMISQWCTSRGIEL---QDRVRVHYLDDDVITEADR 185
           G++TCP++Q+ L H  LTPN++++ +I+ WC S GIEL   Q   R   +     ++ DR
Sbjct: 285 GHKTCPKSQETLLHAGLTPNYVLKSLIALWCESNGIELPQNQGSCRTTKIGGSSSSDCDR 344

Query: 186 DRFLTLIEKMSM-TISEQKAAAKELRMLTKRMPSFRALFGESLDAISLLLSPLCGDKSQS 245
              L+L+EK++  T  +Q+AAA ELR+L KR    R    E+  AI LL+  L     ++
Sbjct: 345 TFVLSLLEKLANGTTEQQRAAAGELRLLAKRNVDNRVCIAEA-GAIPLLVELLSSPDPRT 404

Query: 246 ISIHTDLQEDVITTLLNLSIHDNNKKLVAETPKVIPLLMEALRSGTMETRSNAAAALFTL 305
                  QE  +T LLNLSI++ NK  + +   +   ++E L++G+ME R NAAA LF+L
Sbjct: 405 -------QEHSVTALLNLSINEGNKGAIVDAGAITD-IVEVLKNGSMEARENAAATLFSL 464

Query: 306 SALDSNKAIIGKAGALKPLIDLLDEGHPLSIKDAASAIFNLCIIHENKARAVRDGAVRVL 365
           S +D NK  IG AGA++ LI LL+EG     KDAA+AIFNLCI   NK+RAV+ G V  L
Sbjct: 465 SVIDENKVAIGAAGAIQALISLLEEGTRRGKKDAATAIFNLCIYQGNKSRAVKGGIVDPL 524

Query: 366 --LKKIMNQMHVDELLAILAMLSSHQKAIEEIGELGAVPFLLRIIRESPCSRNKENCIVI 425
             L K      VDE LAILA+LS++Q+    I E  ++P L+ IIR +   RN+EN   I
Sbjct: 525 TRLLKDAGGGMVDEALAILAILSTNQEGKTAIAEAESIPVLVEIIR-TGSPRNRENAAAI 584

Query: 426 IHAICLYDRTKWKDMREEEKCYRTISELAQNGTSRAKRKASGILERLNRVVNMTHT 460
           +  +C+ +  +    RE       + EL +NGT RAKRKA+ +LE + +   +  T
Sbjct: 585 LWYLCIGNIERLNVAREVGADV-ALKELTENGTDRAKRKAASLLELIQQTEGVAVT 629

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRT02.9e-15661.21U-box domain-containing protein 9 OS=Arabidopsis thaliana OX=3702 GN=PUB9 PE=1 S... [more]
Q3E9F72.9e-7940.09Putative U-box domain-containing protein 46 OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q5VRH98.1e-7439.91U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=P... [more]
Q8VZ401.1e-7338.45U-box domain-containing protein 14 OS=Arabidopsis thaliana OX=3702 GN=PUB14 PE=1... [more]
Q9ZV317.8e-6940.58U-box domain-containing protein 12 OS=Arabidopsis thaliana OX=3702 GN=PUB12 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1HUJ64.5e-23793.48RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466836 P... [more]
A0A6J1DYS94.5e-23793.48RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102586... [more]
A0A6J1HKK54.5e-23793.48RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111463870... [more]
A0A5D3DRP91.9e-23592.19RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CDM91.9e-23592.19RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103499224 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_038888241.13.7e-24998.04U-box domain-containing protein 9 [Benincasa hispida][more]
XP_022967253.19.4e-23793.48U-box domain-containing protein 9-like [Cucurbita maxima][more]
XP_022963574.19.4e-23793.48U-box domain-containing protein 9-like [Cucurbita moschata][more]
XP_022159455.19.4e-23793.48U-box domain-containing protein 9 [Momordica charantia][more]
XP_023554208.12.1e-23693.48U-box domain-containing protein 9-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G07360.12.1e-15761.21plant U-box 9 [more]
AT3G07360.24.1e-10560.79plant U-box 9 [more]
AT3G07360.34.1e-10560.79plant U-box 9 [more]
AT5G18320.12.0e-8040.09ARM repeat superfamily protein [more]
AT3G54850.17.5e-7538.45plant U-box 14 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 246..287
e-value: 12.0
score: 12.7
coord: 288..328
e-value: 0.061
score: 22.4
coord: 368..409
e-value: 120.0
score: 4.9
IPR000225ArmadilloPFAMPF00514Armcoord: 291..327
e-value: 1.3E-6
score: 28.2
IPR003613U box domainSMARTSM00504Ubox_2coord: 76..139
e-value: 2.5E-31
score: 120.1
IPR003613U box domainPFAMPF04564U-boxcoord: 74..143
e-value: 3.8E-22
score: 78.2
IPR003613U box domainPROSITEPS51698U_BOXcoord: 72..146
score: 40.143551
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 210..355
e-value: 2.5E-22
score: 81.2
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 356..455
e-value: 5.6E-8
score: 34.3
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 72..146
e-value: 4.6E-28
score: 98.9
NoneNo IPR availablePANTHERPTHR23315:SF253U-BOX DOMAIN-CONTAINING PROTEIN 9coord: 6..458
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 6..458
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 77..119
e-value: 2.27748E-24
score: 93.067
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 73..149
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 182..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G011400.1Lsi05G011400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity