Lsi04G001520 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G001520
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	iron-sulfur assembly protein IscA-like 1, mitochondrial
Location	chr04: 1716919 .. 1719404 (-)
RNA-Seq Expression	Lsi04G001520
Synteny	Lsi04G001520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCAGCTTCTCAGAAGGTCGGACCAGCCGCTCGCCGCCAAATTCTCACTCTGACGGAGGCCGCCGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTCCGTCTTGGCGTCAAGGCGCGCGGTTGTAATGGCTTGTCCTATACTCTCAACTACGCAGGTTCGTAATAACCTAATCGATTTCAACTTTTTATTGATTCTACGCGATTTGTTTTTTACTCATTTTATGTCTGAATTTAAGGATGGGATGAAATTTGTCGATTATATACTCTTTTTTCTTTGAATCGGATGTTGCTGTTGAGATAAATAGGTATTTGGTGTATTTGGATATAACCTAATAGTTATGACGAGATATTTCGGAACTGGAGTCTTTTAGAAGATGAATTATTTATCTTTGAACTGTTTCCAGCTCCAATTTCCAAAAATTAGGATTTTAAGACTGAGGATCTATTGTGATGATTAAACGTGGAAAACTAGAAGGCTAAATGATTCTGTAATTGTTATAACCGGAATTCGGCCTGAGTATGATATGTAAGTTGTTCGAAGATGAGCTGTTGAGTTCTTGCTAATGCTAACCTTAACAACGGCAAATCTGAAAGATAAAACTGACATGCCTGAATGCTTAAGTCAATAGACTAGAGATGATTTTACATGCAGGGACCTATATATGATAAGAGAATTGACTTAAATATATGATAAGAGAATTGACTTAACTAAACTACTTCAGTCTGTAAATCATTCTGGATCTTATAGAATCAGTTATGAATTAATGGTGTTATGGGAGGGAATTTTGGATTCGACGATTTACTTAACAAACTTATGACACAGGGATTAGTGTGTTATTGATATATTACAATGCCAAACTAAATGCATGTTCCACCCAAATTTAAGGTGAACATTTGCTTCAATCAAGTACCATTTACTTTGGGGTGTATGTTGGCTATTGCGAGAACATAGCAGGATTGGACATTGATTAAATTTACTTGAGAACCGTTGTCTCTTTCTCCTGCTATGGTTAATTAGAATGTTATCGGACCAAAGTGCTGTTTCTATTCAATTAGAATATAAACGGGTTAGCAAGCCTTCCATGGGTCAACTTGGAGAGAATAGTTATTAACATGTTAGATGGTCAGAAATTTGAGACTTTATACACTTCGTGGTTTGGGACTTTTTATTGCCTGTGTTTATTTAATACGGCTATCTTCTCAGTTAACAGTATTTACAATTCAGTTTTCTATGGAGTTGCTCATGATTTGAGAATCTTCTAACTCAGCATTACCTCAGTTTTATAATCTCATTATCATTAACATTTTCTTTGTGCAAGAAATAGGGTTCAACCTTTATCTCTCTTTTGGCGTAAATTGACTATAGTTATGATCTCTATCTTTCAGAATACTTCAAGATTTGCAGTATTTCATTTAACGGTTCCGTTTCCAAATATTTCAGATGAGAAAGGGAAGTTTGATGAACTGGTTGAGGATCAAAATGTGAAGATACTGATTGATCCAAAGGCTTTGATGCACGTAATAGGAACAAAGATGGACTTTGTCGATGACAAATTGAGGTATTTGAGAAATCTCTTTTCTACAATTGTATGTTTTCAGCTCTATATTTCCATCACTATGTTGATGAAATATCATGCTAGTTAGTTACAACATCAGGGTGAAATGTAAAAGCTTGTGTACTTATATTTAAATGTCCACGAGAGTAAGCATCTGCATCTGTACGTAAACCTATTGTTTCACCCTTATCATGACTCCTCAGAAACGCTACTTTGTTATTCCAGAATCTAAACATATTTTCAGAGGTTTTAATCAGCTGTAAGCCAACCCCATTCTGAATTTGCTCGTCAATCAATTTTCTATAGTGCTGGAGGTATTTGCAATATGCAAGGACCACAGTATTCGTTTGCATGTACATGATAAGGGGTTATCTTTGCTTGAGAATGGGTTCAGATCATCTTTACTCTTTAGCATCCATAAGCTGGTGAACATAATTAATACAACTTAAGAGAGAAAGTATATTAGTTTCAGCAAGCATACCTCATTAGATAAGCCACCGATTACTATTTCAAAGATCGAACTCTTTAGCATCCATAAGCGGGTAAATATCATTAATACAATTTAAGATAGAACTGTTAAGTATATTTGTTTAGGGTTTAGTCAATCCAAGCACACTTCAGTAGATAAGATACTAATTATTATTCCAAAGGTTGATGGTTCGATCTTCTATCTTGCAATTGTTGAACTAAAGAAAATAAGTTTATTAGATTCAAGTTATCTCGAGTCTCGATGCGTACTGCTATATTAAGTGGCCAGTTAACCTTATTTTTTCTCTCTCTTCTTGGTAGATCTGAGTTTATCTTTATCAATCCAAATTCCAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAATGA

mRNA sequence

ATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCAGCTTCTCAGAAGGTCGGACCAGCCGCTCGCCGCCAAATTCTCACTCTGACGGAGGCCGCCGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTCCGTCTTGGCGTCAAGGCGCGCGGTTGTAATGGCTTGTCCTATACTCTCAACTACGCAGGTTCAATGTTATCGGACCAAAGTGCTGTTTCTATTCAATTAGAATATAAACGGAATACTTCAAGATTTGCAGTATTTCATTTAACGGTTCCGTTTCCAAATATTTCAGATGAGAAAGGGAAGTTTGATGAACTGGTTGAGGATCAAAATGTGAAGATACTGATTGATCCAAAGGCTTTGATGCACGTAATAGGAACAAAGATGGACTTTGTCGATGACAAATTGAGGTATTTGAGAAATCTCTTTTCTACAATTAAACGCTACTTTGTTATTCCAGAATCTAAACATATTTTCAGAGGTTTTAATCAGCTATCTGAGTTTATCTTTATCAATCCAAATTCCAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAATGA

Coding sequence (CDS)

ATGGCAGCTTCTTCGTCTTCCTTTTTCGCCGCAGCTTCTCAGAAGGTCGGACCAGCCGCTCGCCGCCAAATTCTCACTCTGACGGAGGCCGCCGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTCCGTCTTGGCGTCAAGGCGCGCGGTTGTAATGGCTTGTCCTATACTCTCAACTACGCAGGTTCAATGTTATCGGACCAAAGTGCTGTTTCTATTCAATTAGAATATAAACGGAATACTTCAAGATTTGCAGTATTTCATTTAACGGTTCCGTTTCCAAATATTTCAGATGAGAAAGGGAAGTTTGATGAACTGGTTGAGGATCAAAATGTGAAGATACTGATTGATCCAAAGGCTTTGATGCACGTAATAGGAACAAAGATGGACTTTGTCGATGACAAATTGAGGTATTTGAGAAATCTCTTTTCTACAATTAAACGCTACTTTGTTATTCCAGAATCTAAACATATTTTCAGAGGTTTTAATCAGCTATCTGAGTTTATCTTTATCAATCCAAATTCCAAAGGTCAATGTGGCTGTGGGGAGTCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAATGA

Protein sequence

MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNSKGQCGCGESFMTTGSAEANKK

Homology

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BLAST of Lsi04G001520 vs. ExPASy Swiss-Prot
Match: Q8LBM4 (Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g16710 PE=2 SV=2)

HSP 1 Score: 177.6 bits (449), Expect = 1.4e-43
Identity = 101/196 (51.53%), Postives = 120/196 (61.22%), Query Frame = 0


Query: 5   SSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA+ +VGPA R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKILIDPK 124
           YA                                    DEKGKFDELVE++ V+IL++PK
Sbjct: 63  YA------------------------------------DEKGKFDELVEEKGVRILVEPK 122

Query: 125 ALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNSKGQC 184
           ALMHVIGTKMDFVDDKLR                            SEF+FINPNS+GQC
Sbjct: 123 ALMHVIGTKMDFVDDKLR----------------------------SEFVFINPNSQGQC 134

Query: 185 GCGESFMTTGSAEANK 201
           GCGESFMTT ++ A +
Sbjct: 183 GCGESFMTTSTSSAKQ 134

[Close]

BLAST of Lsi04G001520 vs. ExPASy Swiss-Prot
Match: Q8L8C0 (Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g36260 PE=3 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 5.7e-32
Identity = 81/173 (46.82%), Postives = 99/173 (57.23%), Query Frame = 0


Query: 21  RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYAGSMLSDQSAVSIQL 80
           R+Q+L L++ AAARIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA              
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYA-------------- 61

Query: 81  EYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDK 140
                                  EKGKFDE+VE++ VKIL+DPKA+MHVIGT+MDFVDDK
Sbjct: 62  ----------------------QEKGKFDEVVEEKGVKILVDPKAVMHVIGTEMDFVDDK 109

Query: 141 LRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNSKGQCGCGESFMTT 194
           LR                            SEF+F+NPN+  +CGCGESF TT
Sbjct: 122 LR----------------------------SEFVFVNPNAT-KCGCGESFTTT 109

[Close]

BLAST of Lsi04G001520 vs. ExPASy Swiss-Prot
Match: Q07821 (Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.4e-17
Identity = 58/171 (33.92%), Postives = 78/171 (45.61%), Query Frame = 0


Query: 21  RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYAGSMLSDQSAVSIQL 80
           R+ ++TL+  A   +R LL Q +   +R+  + RGC+GL+Y L Y               
Sbjct: 143 RKALITLSPKAIKHLRALLAQPEPKLIRVSARNRGCSGLTYDLQYI-------------- 202

Query: 81  EYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDK 140
                                  E GKFDE+VE   VKI+ID KAL  +IG++MD++DDK
Sbjct: 203 ----------------------TEPGKFDEVVEQDGVKIVIDSKALFSIIGSEMDWIDDK 249

Query: 141 LRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNSKGQCGCGESFM 192
           L                             S+F+F NPNSKG CGCGESFM
Sbjct: 263 L----------------------------ASKFVFKNPNSKGTCGCGESFM 249

[Close]

BLAST of Lsi04G001520 vs. ExPASy Swiss-Prot
Match: Q8T3X9 (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=MagR PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 62/170 (36.47%), Postives = 76/170 (44.71%), Query Frame = 0


Query: 22  RQILTLTEAAAARIRQLLQQR-QRPFLRLGVKARGCNGLSYTLNYAGSMLSDQSAVSIQL 81
           R  LTLT AA  RI+ LLQ +     L++GV+ RGCNGLSYTL+YA              
Sbjct: 23  RAALTLTPAAVLRIKTLLQDKPDMVGLKVGVRQRGCNGLSYTLDYA-------------- 82

Query: 82  EYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDK 141
                                  +K K DE V    VK+ ID KA + ++GT+MDFV+ K
Sbjct: 83  ----------------------SQKDKLDEEVVQDGVKVFIDKKAQLSLLGTEMDFVESK 128

Query: 142 LRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNSKGQCGCGESF 191
           L                             SEF+F NPN KG CGCGESF
Sbjct: 143 LS----------------------------SEFVFNNPNIKGTCGCGESF 128

[Close]

BLAST of Lsi04G001520 vs. ExPASy Swiss-Prot
Match: Q54VS1 (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=isca1 PE=3 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.4e-16
Identity = 54/182 (29.67%), Postives = 90/182 (49.45%), Query Frame = 0


Query: 15  KVGPAARRQILTLTEAAAARIRQLLQQRQRP---FLRLGVKARGCNGLSYTLNYAGSMLS 74
           K+G  +++ I ++T++A  R+++++  ++      LRLG+K RGC+G+SYTL++A     
Sbjct: 8   KLGKPSKKAIFSMTDSALKRVKEIMNDKKIDNCIGLRLGIKERGCSGMSYTLDFA----- 67

Query: 75  DQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDE-LVEDQNVKILIDPKALMHVI 134
                                           +K KFDE +V D+++ I++D KAL+ VI
Sbjct: 68  -------------------------------TQKNKFDETVVADKDINIIVDSKALLSVI 125

Query: 135 GTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNSKGQCGCGESF 193
           GT+MD++++ ++                             EFIFINPN+   CGCGESF
Sbjct: 128 GTEMDYIEEPIK----------------------------KEFIFINPNATNTCGCGESF 125

[Close]

BLAST of Lsi04G001520 vs. ExPASy TrEMBL
Match: A0A6J1JJH4 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484969 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.5e-56
Identity = 136/201 (67.66%), Postives = 137/201 (68.16%), Query Frame = 0


Query: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKIL 120
           YTLNYA                                    DEKGKFDELVEDQNVKIL
Sbjct: 79  YTLNYA------------------------------------DEKGKFDELVEDQNVKIL 138

Query: 121 IDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNS 180
           I+PKALMHVIGTKMDFVDDKLR                            SEFIFINPNS
Sbjct: 139 IEPKALMHVIGTKMDFVDDKLR----------------------------SEFIFINPNS 155

Query: 181 KGQCGCGESFMTTGSAEANKK 202
           KGQCGCGESFMTTGSAEANKK
Sbjct: 199 KGQCGCGESFMTTGSAEANKK 155

[Close]

BLAST of Lsi04G001520 vs. ExPASy TrEMBL
Match: A0A5A7SVZ7 (Iron-sulfur assembly protein IscA-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001970 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.2e-55
Identity = 134/201 (66.67%), Postives = 134/201 (66.67%), Query Frame = 0


Query: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQK GPA RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKIL 120
           YTLNYA                                    DEKGKFDELVEDQNVKIL
Sbjct: 61  YTLNYA------------------------------------DEKGKFDELVEDQNVKIL 120

Query: 121 IDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNS 180
           IDPKALMHVIGTKMDFVDDKLR                            SEFIFINPNS
Sbjct: 121 IDPKALMHVIGTKMDFVDDKLR----------------------------SEFIFINPNS 137

Query: 181 KGQCGCGESFMTTGSAEANKK 202
           KGQCGCGESFMTT SAEANKK
Sbjct: 181 KGQCGCGESFMTTSSAEANKK 137

[Close]

BLAST of Lsi04G001520 vs. ExPASy TrEMBL
Match: A0A0A0KTF0 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G614650 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.2e-55
Identity = 134/201 (66.67%), Postives = 135/201 (67.16%), Query Frame = 0


Query: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQK GPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKIL 120
           YTLNYA                                    DEKGKFDELVEDQNVKIL
Sbjct: 61  YTLNYA------------------------------------DEKGKFDELVEDQNVKIL 120

Query: 121 IDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNS 180
           I+PKALMHVIGTKMDFVDDKLR                            SEFIFINPNS
Sbjct: 121 IEPKALMHVIGTKMDFVDDKLR----------------------------SEFIFINPNS 137

Query: 181 KGQCGCGESFMTTGSAEANKK 202
           KGQCGCGESFMTT SAEANKK
Sbjct: 181 KGQCGCGESFMTTSSAEANKK 137

[Close]

BLAST of Lsi04G001520 vs. ExPASy TrEMBL
Match: A0A6J1GZ87 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111458498 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.2e-55
Identity = 134/201 (66.67%), Postives = 136/201 (67.66%), Query Frame = 0


Query: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAASQKVGPAARR+ILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRKILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKIL 120
           YTLNYA                                    DEKGKFDELVEDQNVKIL
Sbjct: 61  YTLNYA------------------------------------DEKGKFDELVEDQNVKIL 120

Query: 121 IDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNS 180
           I+PKALMHVIGTKMDFVDDKLR                            SEFIFINPNS
Sbjct: 121 IEPKALMHVIGTKMDFVDDKLR----------------------------SEFIFINPNS 137

Query: 181 KGQCGCGESFMTTGSAEANKK 202
           KGQCGCGESFMTTGSAEANKK
Sbjct: 181 KGQCGCGESFMTTGSAEANKK 137

[Close]

BLAST of Lsi04G001520 vs. ExPASy TrEMBL
Match: A0A1S3BFV2 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103489389 PE=4 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.2e-55
Identity = 134/201 (66.67%), Postives = 134/201 (66.67%), Query Frame = 0


Query: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQK GPA RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKIL 120
           YTLNYA                                    DEKGKFDELVEDQNVKIL
Sbjct: 61  YTLNYA------------------------------------DEKGKFDELVEDQNVKIL 120

Query: 121 IDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNS 180
           IDPKALMHVIGTKMDFVDDKLR                            SEFIFINPNS
Sbjct: 121 IDPKALMHVIGTKMDFVDDKLR----------------------------SEFIFINPNS 137

Query: 181 KGQCGCGESFMTTGSAEANKK 202
           KGQCGCGESFMTT SAEANKK
Sbjct: 181 KGQCGCGESFMTTSSAEANKK 137

[Close]

BLAST of Lsi04G001520 vs. NCBI nr
Match: XP_038891787.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 230.7 bits (587), Expect = 1.1e-56
Identity = 137/201 (68.16%), Postives = 137/201 (68.16%), Query Frame = 0


Query: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKIL 120
           YTLNYA                                    DEKGKFDELVEDQNVKIL
Sbjct: 61  YTLNYA------------------------------------DEKGKFDELVEDQNVKIL 120

Query: 121 IDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNS 180
           IDPKALMHVIGTKMDFVDDKLR                            SEFIFINPNS
Sbjct: 121 IDPKALMHVIGTKMDFVDDKLR----------------------------SEFIFINPNS 137

Query: 181 KGQCGCGESFMTTGSAEANKK 202
           KGQCGCGESFMTTGSAEANKK
Sbjct: 181 KGQCGCGESFMTTGSAEANKK 137

[Close]

BLAST of Lsi04G001520 vs. NCBI nr
Match: XP_022987453.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 229.2 bits (583), Expect = 3.2e-56
Identity = 136/201 (67.66%), Postives = 137/201 (68.16%), Query Frame = 0


Query: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKIL 120
           YTLNYA                                    DEKGKFDELVEDQNVKIL
Sbjct: 79  YTLNYA------------------------------------DEKGKFDELVEDQNVKIL 138

Query: 121 IDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNS 180
           I+PKALMHVIGTKMDFVDDKLR                            SEFIFINPNS
Sbjct: 139 IEPKALMHVIGTKMDFVDDKLR----------------------------SEFIFINPNS 155

Query: 181 KGQCGCGESFMTTGSAEANKK 202
           KGQCGCGESFMTTGSAEANKK
Sbjct: 199 KGQCGCGESFMTTGSAEANKK 155

[Close]

BLAST of Lsi04G001520 vs. NCBI nr
Match: KAG7032017.1 (Iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 228.8 bits (582), Expect = 4.2e-56
Identity = 135/201 (67.16%), Postives = 137/201 (68.16%), Query Frame = 0


Query: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKIL 120
           YTLNYA                                    DEKGKFDELVEDQNVKIL
Sbjct: 79  YTLNYA------------------------------------DEKGKFDELVEDQNVKIL 138

Query: 121 IDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNS 180
           I+PKALMHVIGTKMDF+DDKLR                            SEFIFINPNS
Sbjct: 139 IEPKALMHVIGTKMDFIDDKLR----------------------------SEFIFINPNS 155

Query: 181 KGQCGCGESFMTTGSAEANKK 202
           KGQCGCGESFMTTGSAEANKK
Sbjct: 199 KGQCGCGESFMTTGSAEANKK 155

[Close]

BLAST of Lsi04G001520 vs. NCBI nr
Match: XP_022956980.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 225.3 bits (573), Expect = 4.6e-55
Identity = 134/201 (66.67%), Postives = 136/201 (67.66%), Query Frame = 0


Query: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAASQKVGPAARR+ILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRKILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKIL 120
           YTLNYA                                    DEKGKFDELVEDQNVKIL
Sbjct: 61  YTLNYA------------------------------------DEKGKFDELVEDQNVKIL 120

Query: 121 IDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNS 180
           I+PKALMHVIGTKMDFVDDKLR                            SEFIFINPNS
Sbjct: 121 IEPKALMHVIGTKMDFVDDKLR----------------------------SEFIFINPNS 137

Query: 181 KGQCGCGESFMTTGSAEANKK 202
           KGQCGCGESFMTTGSAEANKK
Sbjct: 181 KGQCGCGESFMTTGSAEANKK 137

[Close]

BLAST of Lsi04G001520 vs. NCBI nr
Match: XP_004135035.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis sativus] >KGN52189.1 hypothetical protein Csa_009361 [Cucumis sativus])

HSP 1 Score: 225.3 bits (573), Expect = 4.6e-55
Identity = 134/201 (66.67%), Postives = 135/201 (67.16%), Query Frame = 0


Query: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFFAAASQK GPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKIL 120
           YTLNYA                                    DEKGKFDELVEDQNVKIL
Sbjct: 61  YTLNYA------------------------------------DEKGKFDELVEDQNVKIL 120

Query: 121 IDPKALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNS 180
           I+PKALMHVIGTKMDFVDDKLR                            SEFIFINPNS
Sbjct: 121 IEPKALMHVIGTKMDFVDDKLR----------------------------SEFIFINPNS 137

Query: 181 KGQCGCGESFMTTGSAEANKK 202
           KGQCGCGESFMTT SAEANKK
Sbjct: 181 KGQCGCGESFMTTSSAEANKK 137

[Close]

BLAST of Lsi04G001520 vs. TAIR 10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 181.4 bits (459), Expect = 7.1e-46
Identity = 102/196 (52.04%), Postives = 122/196 (62.24%), Query Frame = 0


Query: 5   SSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA+ +VGPA R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKILIDPK 124
           YAG                                  +DEKGKFDELVE++ V+IL++PK
Sbjct: 63  YAGKTSG------------------------------NDEKGKFDELVEEKGVRILVEPK 122

Query: 125 ALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNSKGQC 184
           ALMHVIGTKMDFVDDKLR                            SEF+FINPNS+GQC
Sbjct: 123 ALMHVIGTKMDFVDDKLR----------------------------SEFVFINPNSQGQC 140

Query: 185 GCGESFMTTGSAEANK 201
           GCGESFMTT ++ A +
Sbjct: 183 GCGESFMTTSTSSAKQ 140

[Close]

BLAST of Lsi04G001520 vs. TAIR 10
Match: AT2G16710.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.0e-44
Identity = 101/196 (51.53%), Postives = 120/196 (61.22%), Query Frame = 0


Query: 5   SSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA+ +VGPA R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKILIDPK 124
           YA                                    DEKGKFDELVE++ V+IL++PK
Sbjct: 63  YA------------------------------------DEKGKFDELVEEKGVRILVEPK 122

Query: 125 ALMHVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNSKGQC 184
           ALMHVIGTKMDFVDDKLR                            SEF+FINPNS+GQC
Sbjct: 123 ALMHVIGTKMDFVDDKLR----------------------------SEFVFINPNSQGQC 134

Query: 185 GCGESFMTTGSAEANK 201
           GCGESFMTT ++ A +
Sbjct: 183 GCGESFMTTSTSSAKQ 134

[Close]

BLAST of Lsi04G001520 vs. TAIR 10
Match: AT2G16710.2 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 143.7 bits (361), Expect = 1.6e-34
Identity = 79/138 (57.25%), Postives = 93/138 (67.39%), Query Frame = 0


Query: 5   SSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S   AAA+ +VGPA R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YAGSMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKILIDPK 124
           YA                                    DEKGKFDELVE++ V+IL++PK
Sbjct: 63  YA------------------------------------DEKGKFDELVEEKGVRILVEPK 104

Query: 125 ALMHVIGTKMDFVDDKLR 143
           ALMHVIGTKMDFVDDKLR
Sbjct: 123 ALMHVIGTKMDFVDDKLR 104

[Close]

BLAST of Lsi04G001520 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 139.0 bits (349), Expect = 4.1e-33
Identity = 81/173 (46.82%), Postives = 99/173 (57.23%), Query Frame = 0


Query: 21  RRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYAGSMLSDQSAVSIQL 80
           R+Q+L L++ AAARIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA              
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYA-------------- 61

Query: 81  EYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDK 140
                                  EKGKFDE+VE++ VKIL+DPKA+MHVIGT+MDFVDDK
Sbjct: 62  ----------------------QEKGKFDEVVEEKGVKILVDPKAVMHVIGTEMDFVDDK 109

Query: 141 LRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNSKGQCGCGESFMTT 194
           LR                            SEF+F+NPN+  +CGCGESF TT
Sbjct: 122 LR----------------------------SEFVFVNPNAT-KCGCGESFTTT 109

[Close]

BLAST of Lsi04G001520 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 42.7 bits (99), Expect = 4.0e-04
Identity = 43/183 (23.50%), Postives = 72/183 (39.34%), Query Frame = 0


Query: 11  AASQKVGPAAR--RQILTLTEAAAARIRQLLQQRQRPF-LRLGVKARGCNGLSYTLNYAG 70
           +AS    PA    +  ++L+E A   + ++  +R     LR+GVK  GC+G+SYT+++  
Sbjct: 56  SASVPAAPAMEGLKPAISLSENALKHLSKMRSERGEDLCLRIGVKQGGCSGMSYTMDF-- 115

Query: 71  SMLSDQSAVSIQLEYKRNTSRFAVFHLTVPFPNISDEKGKFDELVEDQNVKILIDPKALM 130
                        E + N             P+        D  +E Q   I+ DPK+++
Sbjct: 116 -------------ENRANAR-----------PD--------DSTIEYQGFTIVCDPKSML 175

Query: 131 HVIGTKMDFVDDKLRYLRNLFSTIKRYFVIPESKHIFRGFNQLSEFIFINPNSKGQCGCG 190
            + G ++D+ D  +                               F F NPN+   CGCG
Sbjct: 176 FLFGMQLDYSDALIG----------------------------GGFSFSNPNATQTCGCG 176

The following BLAST results are available for this feature:

BLAST of Lsi04G001520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LBM4	1.4e-43	51.53	Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana ...	[more]
Q8L8C0	5.7e-32	46.82	Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana ...	[more]
Q07821	1.4e-17	33.92	Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 /...	[more]
Q8T3X9	1.5e-16	36.47	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster...	[more]
Q54VS1	3.4e-16	29.67	Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideu...	[more]

BLAST of Lsi04G001520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JJH4	1.5e-56	67.66	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3...	[more]
A0A5A7SVZ7	2.2e-55	66.67	Iron-sulfur assembly protein IscA-like 1 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A0A0KTF0	2.2e-55	66.67	Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G614650...	[more]
A0A6J1GZ87	2.2e-55	66.67	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX...	[more]
A0A1S3BFV2	2.2e-55	66.67	iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucumis melo OX=3656 ...	[more]

BLAST of Lsi04G001520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891787.1	1.1e-56	68.16	iron-sulfur assembly protein IscA-like 1, mitochondrial isoform X1 [Benincasa hi...	[more]
XP_022987453.1	3.2e-56	67.66	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima]	[more]
KAG7032017.1	4.2e-56	67.16	Iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita argyrosperma ...	[more]
XP_022956980.1	4.6e-55	66.67	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata]	[more]
XP_004135035.1	4.6e-55	66.67	iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucumis sativus] >KGN52...	[more]

BLAST of Lsi04G001520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G16710.3	7.1e-46	52.04	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.1	1.0e-44	51.53	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G16710.2	1.6e-34	57.25	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	4.1e-33	46.82	Iron-sulphur cluster biosynthesis family protein	[more]
AT1G10500.1	4.0e-04	23.50	chloroplast-localized ISCA-like protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035903	HesB-like domain superfamily	GENE3D	2.60.300.12		coord: 24..145 e-value: 2.5E-20 score: 74.3
IPR035903	HesB-like domain superfamily	SUPERFAMILY	89360	HesB-like domain	coord: 24..142
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 100..142
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 1..67
None	No IPR available	PANTHER	PTHR10072:SF60	IRON-SULFUR ASSEMBLY PROTEIN ISCA-LIKE 3, MITOCHONDRIAL	coord: 171..200
None	No IPR available	PANTHER	PTHR10072:SF60	IRON-SULFUR ASSEMBLY PROTEIN ISCA-LIKE 3, MITOCHONDRIAL	coord: 1..67
None	No IPR available	PANTHER	PTHR10072:SF60	IRON-SULFUR ASSEMBLY PROTEIN ISCA-LIKE 3, MITOCHONDRIAL	coord: 100..142
None	No IPR available	PANTHER	PTHR10072	IRON-SULFUR CLUSTER ASSEMBLY PROTEIN	coord: 171..200
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 173..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G001520.1	Lsi04G001520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0051536	iron-sulfur cluster binding