Lsi02G011250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi02G011250
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Thymidine kinase
Location	chr02: 13150789 .. 13154523 (+)
RNA-Seq Expression	Lsi02G011250
Synteny	Lsi02G011250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATCCATAAAAAAAAAAAAAAAACGGAGTCCCCATTCACTCTCCAAAGTGCAAACCAAGAACCCTCGATGTTCTACAAATTGCAGACATTTCTCATCTGCAAAGCCAAAACCCCATTAAAACCCTCAACCAAAATCTCATGTTAACCATTTCAAAGATGAAATCCTTGGTATCTTCGTCTTCTTTCTCAACCCTCTCGCCCTATTTCCCCATTACCGCTTCTCCAACCTTCTTTTCTTTGCCTTCTAAATCTACGCAATGCAGCCCTATATTCACCCAAGTCTCCAATTACTTCACTTTCAAAACACCCAGAATTTTGTTTCCCTCAAAAGGGATTTCTTCAGCCCAGAATCGAACAGGGCAAATCGCAGCCACTTTGTCACCGCCCTCCGGCGAGATTCATGTGATTGTGGGCCCGATGTTTGCTGGGAAGACAACCACTCTTCTTCGGCGGATTCAGTCCGAGAGCAGCAATGGCAGGTCTTTTATGCTCTGCTTTTATGCTTCCGTTTATTAATCGAATGGGTTGCGAGCGATTAGTGTAGTGTTTTTTTCTAGCCTTATTTCAACTTGTTTCCCTTATATTTTTTAACTTTTTGCAACTAATGGAAACTGGGTTTTATACTTATGACAATAGATAGTGGATTTGCTGTTAGATTGGGAGAATTTTGTTTATCTTTTAATGTATTCTCAGCAATCTCTCTGCATTGGCTTGAATATTGGCATGATGGTATCAATATGAAATGGGAAGAAAATGGCATTGAATTTACAACCTCTTGTGCTATATTTACTTAAATCGAGACTAATATTGAGTGTTTGGGTGGATAAGTTATGGTAAATTTGGTGTTTTATTCTCTATCCCTACAAATTCTCAATAATGGAGTTATGTATATTGGTAATGGATGAGGCTGAAGCTGATATATGTTGTGGGTTATGGCCTCTTGATAGAGAATGAATTAAACGAGTGAATTGGTGAACTAAATTTAGCGATTCTTTGAAGAAAAGCTTTAGAAAAAGAACAAAAAGAAAAAGAAAAAAAATTTGAATCCCAAACTTTCCCTTGCTCCCTTAGCTCTTTTAAAAGATTCTTTCATTTTTTTAGCTCAACACTAGAAGATAGCCTTATTAACATAAGACCTCATGGCTTGGGTTTTTCTTTCAAAGAAGACTGCTAATATGGAAACATTGTTGCTCCAAGTTAGGAAGAGGAAATTTCACACCAAAGGTCTGGGGACAGTTTTCACTCAATCTATAAAATATGGGGACATTTAAGAAGTCAGTAACTTGCATTTACACCATGCTTTCTGCAGAGCATGCACCAAATCAATCTACTATTTGGAGTTAGATCACATGGTTTCAAAATGATACAACTACAAGAGTTCCAATGAGATTTGTGAAATAAAGGCTTAGGAGAACATGTTCAAGTCATAGTGGCCCCCTATCCAAAATTTAATATCATACGAGTTTCTCTGACAATTAAATGTAGTAGAACCAGCAGATTGCTGTCTTGTGAGAATTGTTGAAAGCGCTCAAGTTAGCTGGGATGCTCTGAAAAGAAAAAAGTTGTAGAGTTTTTGATAGATTTAATATTTCTATGCTTTTCTTAGTCTATGCTCAGGTTCTGGATATTGGATTAAGTGCACCATTTCAATTTTTGTGGAATCGGATTATCATATTCAAGACCCTCGATTTCAGCTTTTAATTAATTAGATTTTTTTATTTTTTATTTTTTATTTCCTTATTATTGTGTGTGTGTGTGGGGGGGGGGGGGGGGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGGGGGGGGGGGGGACATCTTGTCTTCCTTTTGTGTCTTTATATTAAAAAAGATTGCCCTACCTTCGTACATTTGAGGTCAAGACAGCATCTTTCCTGCTGAAGGCTTATGATCTATATGCTTGTTGTTGATTGGATATAGTATAAATTTTTGGAAATTTTAGAACCTAATTACTTGGATCAGTTGGATGGAATGTATATTCTGATGTATCTTGATTGATTTAGAAGCTTCTTTTTTCAATACAATTCATAAGAATTATTCTTTCTTCTTTTCATGTATGTTTTTTTAACAGAAGTGTAGCTATAATTAAGTCAAATAAAGACACGAGATATGGATTGGATTCTATTGTGACACATGACGGCATGAAACTGCCTTGCTGGGCATTACCAAACTTAACATCTTTCAAACAGAAATTTGGTCAAGGTGCTTATGACAAGGTATCATAATCCCCCTCTCTTTGTGATATGCCAATGTACTTAAATTCTTCATATCTTGCTTAGACATTTCATTAATTAAATACAAAGTATTCTCATCCTTGTTAAAGGGAGCAAAGTATTTAAATCTATGAGGCTAAAGATTTGGTTTCTAATTAGAATTAGCAGACTGCAGAGGAGACTGATTGAGTAGCCTTCTGAATTTCGGTCACCTTCATAATTGGAATTCCTGATTTTCCTGGTAACAAAATTGTTAATTTAATTATGATATTGGTATGACAGCTAGATGTGATTGGAATTGATGAAGCTCAATTCTTCGACGATCTTTATGATTTCTGTCGTGAAGCTGCTGATATTGATGGCAAAACAGTTATAGTTGCTGGGCTGGATGGGGATTACTTGAGGTATAATCAAATTTACTGGCCTAGTTTTGGTTCATTCGCTAAAATAATTTCAATGAAACTGATGGCCTTTGGCTTTCATATGGTAAAACATTCAATAGAACTTCAGATTGGTTTAAATTGAATACTCATTAGTAACTCAGTTAAAGCTTTTGTGTTGGGGTTTATACAAAAAACTGAACAGGAGGAACTTCGGTTCAGTTCTCAATATAATTCCACTTGCTGATTCCGTAACAAAGTTAACTGCTCGATGTGAGATCTGCGGCAATCGGGCTTTCTTTACATTAAGGAAGACAGAAGAAAAAGAGACCGAGCTGATCGGTGGTGCTGACGTGTATATGCCCGTATGTCGACAACATTACGTCAGTGGGCAAGTAGTGATAGAGACAGCAAGAACTGTAGTAGAATCACACAAGCTTGAGTGTAGGACTCCAGCATAGTTGTTCTTAGTAGGTAGTATCAGGTTTGCAGCATCACATACATCCCCAGTAGCGAACGTCGCAACGTATTAGTTGTCCCGACGGGCGATTTTGGAACTATGATGAGCATAGTTGTAGTATTAGGGATATTTACTCAGAGATTTGAAGGCAAAAATATAGAAGGAAGGTGAAAAGGGGGTTTTGTTGTTGGTTTAGAAAATGGAATTGAGTTGGTTGTGCTTGTATTTTCATGGCTGCTTTGATTGATTGGATTAGATTAGAAGGAAAAATGAGTCAATGTTGTGTCATATGTAAGTATTTGTGTTGAATGAAAGAGGAGATGTAACCAACAAAGTTGAAAATTAATACATTATTGAGGGAGGAGACATTCAAGTGGCAACCATGTGTTTGCTTCGGCAGTTGTTGATCAGCCAGCTGTTGATTTTATTGATTGTAATTTCCATTGCCATATAACAACTACTTTTCATAATTAAAGACATGAAAAGTTGTTAATAATTGAATTAGAGCAAATATTATCCTTTTGAAATTATT

mRNA sequence

CATCCATAAAAAAAAAAAAAAAACGGAGTCCCCATTCACTCTCCAAAGTGCAAACCAAGAACCCTCGATGTTCTACAAATTGCAGACATTTCTCATCTGCAAAGCCAAAACCCCATTAAAACCCTCAACCAAAATCTCATGTTAACCATTTCAAAGATGAAATCCTTGGTATCTTCGTCTTCTTTCTCAACCCTCTCGCCCTATTTCCCCATTACCGCTTCTCCAACCTTCTTTTCTTTGCCTTCTAAATCTACGCAATGCAGCCCTATATTCACCCAAGTCTCCAATTACTTCACTTTCAAAACACCCAGAATTTTGTTTCCCTCAAAAGGGATTTCTTCAGCCCAGAATCGAACAGGGCAAATCGCAGCCACTTTGTCACCGCCCTCCGGCGAGATTCATGTGATTGTGGGCCCGATGTTTGCTGGGAAGACAACCACTCTTCTTCGGCGGATTCAGTCCGAGAGCAGCAATGGCAGAAGTGTAGCTATAATTAAGTCAAATAAAGACACGAGATATGGATTGGATTCTATTGTGACACATGACGGCATGAAACTGCCTTGCTGGGCATTACCAAACTTAACATCTTTCAAACAGAAATTTGGTCAAGGTGCTTATGACAAGCTAGATGTGATTGGAATTGATGAAGCTCAATTCTTCGACGATCTTTATGATTTCTGTCGTGAAGCTGCTGATATTGATGGCAAAACAGTTATAGTTGCTGGGCTGGATGGGGATTACTTGAGGAGGAACTTCGGTTCAGTTCTCAATATAATTCCACTTGCTGATTCCGTAACAAAGTTAACTGCTCGATGTGAGATCTGCGGCAATCGGGCTTTCTTTACATTAAGGAAGACAGAAGAAAAAGAGACCGAGCTGATCGGTGGTGCTGACGTGTATATGCCCGTATGTCGACAACATTACGTCAGTGGGCAAGTAGTGATAGAGACAGCAAGAACTGTAGTAGAATCACACAAGCTTGAGTGTAGGACTCCAGCATAGTTGTTCTTAGTAGGTAGTATCAGGTTTGCAGCATCACATACATCCCCAGTAGCGAACGTCGCAACGTATTAGTTGTCCCGACGGGCGATTTTGGAACTATGATGAGCATAGTTGTAGTATTAGGGATATTTACTCAGAGATTTGAAGGCAAAAATATAGAAGGAAGGTGAAAAGGGGGTTTTGTTGTTGGTTTAGAAAATGGAATTGAGTTGGTTGTGCTTGTATTTTCATGGCTGCTTTGATTGATTGGATTAGATTAGAAGGAAAAATGAGTCAATGTTGTGTCATATGTAAGTATTTGTGTTGAATGAAAGAGGAGATGTAACCAACAAAGTTGAAAATTAATACATTATTGAGGGAGGAGACATTCAAGTGGCAACCATGTGTTTGCTTCGGCAGTTGTTGATCAGCCAGCTGTTGATTTTATTGATTGTAATTTCCATTGCCATATAACAACTACTTTTCATAATTAAAGACATGAAAAGTTGTTAATAATTGAATTAGAGCAAATATTATCCTTTTGAAATTATT

Coding sequence (CDS)

ATGTTAACCATTTCAAAGATGAAATCCTTGGTATCTTCGTCTTCTTTCTCAACCCTCTCGCCCTATTTCCCCATTACCGCTTCTCCAACCTTCTTTTCTTTGCCTTCTAAATCTACGCAATGCAGCCCTATATTCACCCAAGTCTCCAATTACTTCACTTTCAAAACACCCAGAATTTTGTTTCCCTCAAAAGGGATTTCTTCAGCCCAGAATCGAACAGGGCAAATCGCAGCCACTTTGTCACCGCCCTCCGGCGAGATTCATGTGATTGTGGGCCCGATGTTTGCTGGGAAGACAACCACTCTTCTTCGGCGGATTCAGTCCGAGAGCAGCAATGGCAGAAGTGTAGCTATAATTAAGTCAAATAAAGACACGAGATATGGATTGGATTCTATTGTGACACATGACGGCATGAAACTGCCTTGCTGGGCATTACCAAACTTAACATCTTTCAAACAGAAATTTGGTCAAGGTGCTTATGACAAGCTAGATGTGATTGGAATTGATGAAGCTCAATTCTTCGACGATCTTTATGATTTCTGTCGTGAAGCTGCTGATATTGATGGCAAAACAGTTATAGTTGCTGGGCTGGATGGGGATTACTTGAGGAGGAACTTCGGTTCAGTTCTCAATATAATTCCACTTGCTGATTCCGTAACAAAGTTAACTGCTCGATGTGAGATCTGCGGCAATCGGGCTTTCTTTACATTAAGGAAGACAGAAGAAAAAGAGACCGAGCTGATCGGTGGTGCTGACGTGTATATGCCCGTATGTCGACAACATTACGTCAGTGGGCAAGTAGTGATAGAGACAGCAAGAACTGTAGTAGAATCACACAAGCTTGAGTGTAGGACTCCAGCATAG

Protein sequence

MLTISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRILFPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA

Homology

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BLAST of Lsi02G011250 vs. ExPASy Swiss-Prot
Match: O81263 (Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2)

HSP 1 Score: 317.4 bits (812), Expect = 1.7e-85
Identity = 158/227 (69.60%), Postives = 185/227 (81.50%), Query Frame = 0


Query: 62  PSKGISSA-----QNRTGQIAATLSPPS--GEIHVIVGPMFAGKTTTLLRRIQSESSNG- 121
           P + +S+A     ++R G  +A  + PS  GEIHVIVGPMFAGKTT LLRR+Q E+  G 
Sbjct: 37  PVRPVSAAAAAADKSRGGGGSAMEAQPSYPGEIHVIVGPMFAGKTTALLRRVQVEAGTGS 96

Query: 122 RSVAIIKSNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQF 181
           R+VA+IKS+KD RYGLDS+VTHDG K+PCWALP L+SF+ K G  AYDK+DVIGIDEAQF
Sbjct: 97  RNVALIKSDKDNRYGLDSVVTHDGTKMPCWALPELSSFQDKLGTEAYDKVDVIGIDEAQF 156

Query: 182 FDDLYDFCREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRA 241
           FDDL+DFC +AAD DGK V+VAGLDGDY R  FGSVL+IIPLADSVTKLTARCE+CG RA
Sbjct: 157 FDDLHDFCCKAADRDGKIVVVAGLDGDYKRNKFGSVLDIIPLADSVTKLTARCELCGRRA 216

Query: 242 FFTLRKTEEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHK 281
           FFTLRKT E +TELIGGADVYMPVCRQHY+ GQ+VIE  R V++  K
Sbjct: 217 FFTLRKTRETKTELIGGADVYMPVCRQHYLDGQIVIEATRIVLDLEK 263

[Close]

BLAST of Lsi02G011250 vs. ExPASy Swiss-Prot
Match: F4KBF5 (Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 5.9e-83
Identity = 163/274 (59.49%), Postives = 208/274 (75.91%), Query Frame = 0


Query: 7   MKSLVSSSSFSTLSPYFPITASPTFFSLPSK-STQCSPIFTQVSNYFTFKTPRILFPSKG 66
           M++L+S S    L+P+      P+ FS   + S   + I    S   T  T ++   +  
Sbjct: 6   MRTLISPS----LAPFSLHLHKPSLFSTALRFSFSINNITPTNSPPSTISTRKLQTKATR 65

Query: 67  ISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDT 126
           ++S+   + Q  ++ SP  GEIHV+VGPMF+GKTTTLLRRI +E   G+ +AIIKSNKDT
Sbjct: 66  VTSSS--SSQPLSSSSP--GEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDT 125

Query: 127 RYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYD-KLDVIGIDEAQFFDDLYDFCREA 186
           RY  +SIVTHDG K PCW+LP+L+SFK++FG   Y+ +LDVIGIDEAQFF DLY+FCREA
Sbjct: 126 RYCTESIVTHDGEKYPCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREA 185

Query: 187 ADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKE 246
           AD +GKTVIVAGLDGD++RR FGSVL++IP+AD+VTKLT+RCE+CG RA FT+RKTEEKE
Sbjct: 186 ADKEGKTVIVAGLDGDFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKE 245

Query: 247 TELIGGADVYMPVCRQHYVSGQVVIETARTVVES 279
           TELIGGA+VYMPVCR HYV GQ V+ETAR V++S
Sbjct: 246 TELIGGAEVYMPVCRSHYVCGQNVLETARAVLDS 271

[Close]

BLAST of Lsi02G011250 vs. ExPASy Swiss-Prot
Match: Q9S750 (Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.8e-80
Identity = 138/194 (71.13%), Postives = 171/194 (88.14%), Query Frame = 0


Query: 84  SGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCW 143
           SG +HVI+GPMF+GK+T+LLRRI+SE S+GRSVA++KS+KDTRY  DS+VTHDG+  PCW
Sbjct: 30  SGAVHVIMGPMFSGKSTSLLRRIKSEISDGRSVAMLKSSKDTRYAKDSVVTHDGIGFPCW 89

Query: 144 ALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLR 203
           ALP+L SF +KFG  AY+KLDVIGIDEAQFF DLY+FC + AD DGK VIVAGLDGDYLR
Sbjct: 90  ALPDLMSFPEKFGLDAYNKLDVIGIDEAQFFGDLYEFCCKVADDDGKIVIVAGLDGDYLR 149

Query: 204 RNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYV 263
           R+FG+VL+IIP+ADSVTKLTARCE+CG++AFFTLRK  +  TELIGGADVYMPVCR+HY+
Sbjct: 150 RSFGAVLDIIPIADSVTKLTARCEVCGHKAFFTLRKNCDTRTELIGGADVYMPVCRKHYI 209

Query: 264 SGQVVIETARTVVE 278
           +  +VI+ ++ V+E
Sbjct: 210 TNHIVIKASKKVLE 223

[Close]

BLAST of Lsi02G011250 vs. ExPASy Swiss-Prot
Match: P23335 (Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.0e-38
Identity = 81/180 (45.00%), Postives = 115/180 (63.89%), Query Frame = 0


Query: 85  GEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCWA 144
           G IH+I+GPMF+GK+T L+R +        +  +IK +KD RYG D++ THD   +   +
Sbjct: 4   GFIHLILGPMFSGKSTELIRLVNRYQIATYNCRVIKYSKDNRYGNDAVYTHDKCYISAVS 63

Query: 145 LPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLRR 204
             +L   K        D +D++GIDE QFF+D+ +FC   A+  GK VIVA LDG Y R+
Sbjct: 64  TDSLFDIKD-----TLDDVDIVGIDEGQFFNDIVEFCEYIAN-KGKIVIVAALDGTYERK 123

Query: 205 NFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYVS 264
            FG++LN+IPL++ VTKL A C IC   A F+ R ++EKE ELIGG + Y+ VCR  Y++
Sbjct: 124 PFGNILNLIPLSEKVTKLNAICMICHRDASFSKRLSDEKEIELIGGKEKYLSVCRSCYLT 177

[Close]

BLAST of Lsi02G011250 vs. ExPASy Swiss-Prot
Match: P04184 (Thymidine kinase, cytosolic OS=Mus musculus OX=10090 GN=Tk1 PE=1 SV=3)

HSP 1 Score: 150.2 bits (378), Expect = 3.5e-35
Identity = 89/185 (48.11%), Postives = 114/185 (61.62%), Query Frame = 0


Query: 81  SPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHDGM-- 140
           S   G+I VI+GPMF+GK+T L+RR++          +IK  KDTRY  +S  THD    
Sbjct: 15  SKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYS-NSFSTHDRNTM 74

Query: 141 -KLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGL 200
             LP   L ++T  ++  G      + VIGIDE QFF D+ DFC   A+ +GKTVIVA L
Sbjct: 75  DALPACMLRDVT--QESLG------VAVIGIDEGQFFPDIVDFCEMMAN-EGKTVIVAAL 134

Query: 201 DGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMPV 260
           DG + R+ FGS+LN++PLA+SV KLTA C  C   A +T R   EKE E+IGGAD Y  V
Sbjct: 135 DGTFQRKAFGSILNLVPLAESVVKLTAVCMECFREAAYTKRLGLEKEVEVIGGADKYHSV 189

Query: 261 CRQHY 263
           CR  Y
Sbjct: 195 CRLCY 189

[Close]

BLAST of Lsi02G011250 vs. ExPASy TrEMBL
Match: A0A1S4E495 (Thymidine kinase OS=Cucumis melo OX=3656 GN=LOC103501719 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.7e-139
Identity = 249/281 (88.61%), Postives = 263/281 (93.59%), Query Frame = 0


Query: 7   MKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRILFPSKGI 66
           MKSLVSSSSFS LSPYFPI+ASP+FFSLPSKS QC P+FTQVSN+FTFKTP    PSKG 
Sbjct: 1   MKSLVSSSSFSILSPYFPISASPSFFSLPSKSAQCGPMFTQVSNFFTFKTPTTSLPSKGF 60

Query: 67  SSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTR 126
            S QNR  Q  A+LSPPSGEIHVI+GPMFAGKTTTLLRRIQSES NGR+VAIIKSNKDTR
Sbjct: 61  FSTQNRNPQTRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRNVAIIKSNKDTR 120

Query: 127 YGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAAD 186
           YGLDSIVTHDGMKLPCWA+PNL+SFK+KFGQG+YDKLDVIGIDEAQFFDDLYDFC EAAD
Sbjct: 121 YGLDSIVTHDGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAAD 180

Query: 187 IDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETE 246
           IDGKTVIVAGLDGDYLRRNFGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT+EKETE
Sbjct: 181 IDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETE 240

Query: 247 LIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA 288
           LIGGADVYMPVCRQHYVSGQVVIE ARTVVES K+ECRTPA
Sbjct: 241 LIGGADVYMPVCRQHYVSGQVVIEAARTVVESRKVECRTPA 281

[Close]

BLAST of Lsi02G011250 vs. ExPASy TrEMBL
Match: A0A0A0LYL4 (Thymidine kinase OS=Cucumis sativus OX=3659 GN=Csa_1G181480 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.1e-138
Identity = 248/285 (87.02%), Postives = 264/285 (92.63%), Query Frame = 0


Query: 3   TISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRILFP 62
           TIS MKS V SSSFS LSPYFPI+ASP+FFSLPSKS QC P+FTQ+SN+FTFKTP I  P
Sbjct: 69  TISNMKSFVFSSSFSILSPYFPISASPSFFSLPSKSAQCGPMFTQLSNFFTFKTPTISLP 128

Query: 63  SKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSN 122
           SKG  S QNR  Q+ A+LSPPSGEIHVI+GPMFAGKTTTLLRRIQSES NGRSVAIIKSN
Sbjct: 129 SKGFFSTQNRNPQMRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRSVAIIKSN 188

Query: 123 KDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCR 182
           KDTRYGLDSIVTHDGMKLPCWA+PNL+SFK+KFGQG+YDKLDVIGIDEAQFFDDLYDFC 
Sbjct: 189 KDTRYGLDSIVTHDGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCC 248

Query: 183 EAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEE 242
           EAADIDGKTVIVAGLDGDYLRRNFGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT+E
Sbjct: 249 EAADIDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQE 308

Query: 243 KETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA 288
           KETELIGGAD+YMPVCRQHYVSGQV IETARTVVES K+  RTPA
Sbjct: 309 KETELIGGADMYMPVCRQHYVSGQVAIETARTVVESRKVGYRTPA 353

[Close]

BLAST of Lsi02G011250 vs. ExPASy TrEMBL
Match: A0A6J1HQ68 (Thymidine kinase OS=Cucurbita maxima OX=3661 GN=LOC111466371 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 1.7e-133
Identity = 246/288 (85.42%), Postives = 264/288 (91.67%), Query Frame = 0


Query: 1   MLTISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRIL 60
           ML ISKMKS VSSSSFSTLSPYFPITASPT  SL  KSTQC+PIFT +SN+F+ KTPR+ 
Sbjct: 1   MLPISKMKSFVSSSSFSTLSPYFPITASPT-LSLAYKSTQCNPIFTHLSNFFSLKTPRVS 60

Query: 61  FPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 120
            P     S Q RT Q+ A+LSPPSGE+HVIVGPMFAGKTT+LLRRIQSES NGRSVAIIK
Sbjct: 61  LPRNEGFSTQKRTAQMGASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAIIK 120

Query: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDGMKLPCWA+PNL+SFKQKFG+GAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWAVPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 240
           CREAAD+DGKTVIVAGLDGDYLRR+FGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT
Sbjct: 181 CREAADMDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVV-ESHKLECRTPA 288
           EEKETELIGGAD+YMPVCRQHYVSGQVVIETARTVV E+HK+E  T A
Sbjct: 241 EEKETELIGGADMYMPVCRQHYVSGQVVIETARTVVEEAHKVEFTTTA 287

[Close]

BLAST of Lsi02G011250 vs. ExPASy TrEMBL
Match: A0A6J1EBD4 (Thymidine kinase OS=Cucurbita moschata OX=3662 GN=LOC111432595 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.5e-133
Identity = 246/288 (85.42%), Postives = 264/288 (91.67%), Query Frame = 0


Query: 1   MLTISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRIL 60
           ML ISKMKS VSSSSFSTLSPYFPITASPT  SL SKSTQC+PIFT +SN+F+ KT R+ 
Sbjct: 1   MLPISKMKSFVSSSSFSTLSPYFPITASPT-LSLASKSTQCNPIFTHLSNFFSLKTLRVS 60

Query: 61  FPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 120
            P     S Q R+ QI A+LSPPSGE+HVIVGPMFAGKTT+LLRRIQSES NGRSVA+IK
Sbjct: 61  LPRNQGFSNQKRSAQIVASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAVIK 120

Query: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDGMKLPCWALPNL+SFKQKFG+GAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 240
           CREAAD+DGKTVIVAGLDGDYLRR+FGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT
Sbjct: 181 CREAADMDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVV-ESHKLECRTPA 288
           EEKETELIGGAD+YMPVCRQHYVSGQVVIETARTVV E+HK+E  T A
Sbjct: 241 EEKETELIGGADMYMPVCRQHYVSGQVVIETARTVVEEAHKVEFTTTA 287

[Close]

BLAST of Lsi02G011250 vs. ExPASy TrEMBL
Match: A0A6J1CKS9 (Thymidine kinase OS=Momordica charantia OX=3673 GN=LOC111012527 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 5.5e-132
Identity = 243/290 (83.79%), Postives = 258/290 (88.97%), Query Frame = 0


Query: 1   MLTISKMKSLV-SSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRI 60
           M TIS+MK  V SSSSFSTL P  PIT  P FFSL SK T C PIF+Q  N+FTFKTP I
Sbjct: 3   MSTISRMKPFVSSSSSFSTLFPGIPITTPPPFFSLLSKPTHCRPIFSQPVNFFTFKTPTI 62

Query: 61  LFPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAII 120
             P  G+ S QNRTG I A+ SPPSGE+HVIVGPMFAGKTTTLLRRIQSESS+GRSVAII
Sbjct: 63  SLPINGVFSTQNRTGPIGASSSPPSGEVHVIVGPMFAGKTTTLLRRIQSESSDGRSVAII 122

Query: 121 KSNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYD 180
           KSNKDTRYGLDSIVTHDGMKLPCWALPNL+SFKQKFGQGAYDKLDVIGIDEAQFFDDLYD
Sbjct: 123 KSNKDTRYGLDSIVTHDGMKLPCWALPNLSSFKQKFGQGAYDKLDVIGIDEAQFFDDLYD 182

Query: 181 FCREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRK 240
           FCREAADIDGKT+IV+GLDGDYLRR+FGSVL+IIPLADSVTKLTARCEICGNRAFFTLRK
Sbjct: 183 FCREAADIDGKTIIVSGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRK 242

Query: 241 TEEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESH--KLECRTPA 288
           TEEKETELIGGADVYMPVCR+HYVSGQV IE ARTV+ESH  K+EC  PA
Sbjct: 243 TEEKETELIGGADVYMPVCRRHYVSGQVAIEAARTVIESHQDKVECGNPA 292

[Close]

BLAST of Lsi02G011250 vs. NCBI nr
Match: XP_038893570.1 (thymidine kinase a-like [Benincasa hispida])

HSP 1 Score: 533.9 bits (1374), Expect = 8.7e-148
Identity = 266/287 (92.68%), Postives = 272/287 (94.77%), Query Frame = 0


Query: 1   MLTISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRIL 60
           MLTISKMK  VSSSS STLSPYFPITASP+FFSLPSKSTQC P FT VSNY TFKTP I 
Sbjct: 1   MLTISKMKPFVSSSSVSTLSPYFPITASPSFFSLPSKSTQCKPTFTHVSNYLTFKTPIIS 60

Query: 61  FPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 120
            PSKG+ S QNR GQ+ A+ SPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK
Sbjct: 61  LPSKGVFSTQNRIGQMKASFSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 120

Query: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 240
           CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT
Sbjct: 181 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA 288
           EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHK+EC+TPA
Sbjct: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVVESHKIECQTPA 287

[Close]

BLAST of Lsi02G011250 vs. NCBI nr
Match: XP_016903056.1 (PREDICTED: thymidine kinase a-like [Cucumis melo])

HSP 1 Score: 504.6 bits (1298), Expect = 5.6e-139
Identity = 249/281 (88.61%), Postives = 263/281 (93.59%), Query Frame = 0


Query: 7   MKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRILFPSKGI 66
           MKSLVSSSSFS LSPYFPI+ASP+FFSLPSKS QC P+FTQVSN+FTFKTP    PSKG 
Sbjct: 1   MKSLVSSSSFSILSPYFPISASPSFFSLPSKSAQCGPMFTQVSNFFTFKTPTTSLPSKGF 60

Query: 67  SSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTR 126
            S QNR  Q  A+LSPPSGEIHVI+GPMFAGKTTTLLRRIQSES NGR+VAIIKSNKDTR
Sbjct: 61  FSTQNRNPQTRASLSPPSGEIHVILGPMFAGKTTTLLRRIQSESCNGRNVAIIKSNKDTR 120

Query: 127 YGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAAD 186
           YGLDSIVTHDGMKLPCWA+PNL+SFK+KFGQG+YDKLDVIGIDEAQFFDDLYDFC EAAD
Sbjct: 121 YGLDSIVTHDGMKLPCWAIPNLSSFKKKFGQGSYDKLDVIGIDEAQFFDDLYDFCCEAAD 180

Query: 187 IDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETE 246
           IDGKTVIVAGLDGDYLRRNFGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT+EKETE
Sbjct: 181 IDGKTVIVAGLDGDYLRRNFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKTQEKETE 240

Query: 247 LIGGADVYMPVCRQHYVSGQVVIETARTVVESHKLECRTPA 288
           LIGGADVYMPVCRQHYVSGQVVIE ARTVVES K+ECRTPA
Sbjct: 241 LIGGADVYMPVCRQHYVSGQVVIEAARTVVESRKVECRTPA 281

[Close]

BLAST of Lsi02G011250 vs. NCBI nr
Match: XP_022966756.1 (thymidine kinase a-like [Cucurbita maxima])

HSP 1 Score: 485.3 bits (1248), Expect = 3.5e-133
Identity = 246/288 (85.42%), Postives = 264/288 (91.67%), Query Frame = 0


Query: 1   MLTISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRIL 60
           ML ISKMKS VSSSSFSTLSPYFPITASPT  SL  KSTQC+PIFT +SN+F+ KTPR+ 
Sbjct: 1   MLPISKMKSFVSSSSFSTLSPYFPITASPT-LSLAYKSTQCNPIFTHLSNFFSLKTPRVS 60

Query: 61  FPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 120
            P     S Q RT Q+ A+LSPPSGE+HVIVGPMFAGKTT+LLRRIQSES NGRSVAIIK
Sbjct: 61  LPRNEGFSTQKRTAQMGASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAIIK 120

Query: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDGMKLPCWA+PNL+SFKQKFG+GAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWAVPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 240
           CREAAD+DGKTVIVAGLDGDYLRR+FGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT
Sbjct: 181 CREAADMDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVV-ESHKLECRTPA 288
           EEKETELIGGAD+YMPVCRQHYVSGQVVIETARTVV E+HK+E  T A
Sbjct: 241 EEKETELIGGADMYMPVCRQHYVSGQVVIETARTVVEEAHKVEFTTTA 287

[Close]

BLAST of Lsi02G011250 vs. NCBI nr
Match: XP_022925302.1 (thymidine kinase a-like [Cucurbita moschata])

HSP 1 Score: 483.4 bits (1243), Expect = 1.3e-132
Identity = 246/288 (85.42%), Postives = 264/288 (91.67%), Query Frame = 0


Query: 1   MLTISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRIL 60
           ML ISKMKS VSSSSFSTLSPYFPITASPT  SL SKSTQC+PIFT +SN+F+ KT R+ 
Sbjct: 1   MLPISKMKSFVSSSSFSTLSPYFPITASPT-LSLASKSTQCNPIFTHLSNFFSLKTLRVS 60

Query: 61  FPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 120
            P     S Q R+ QI A+LSPPSGE+HVIVGPMFAGKTT+LLRRIQSES NGRSVA+IK
Sbjct: 61  LPRNQGFSNQKRSAQIVASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAVIK 120

Query: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDGMKLPCWALPNL+SFKQKFG+GAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 240
           CREAAD+DGKTVIVAGLDGDYLRR+FGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT
Sbjct: 181 CREAADMDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVV-ESHKLECRTPA 288
           EEKETELIGGAD+YMPVCRQHYVSGQVVIETARTVV E+HK+E  T A
Sbjct: 241 EEKETELIGGADMYMPVCRQHYVSGQVVIETARTVVEEAHKVEFTTTA 287

[Close]

BLAST of Lsi02G011250 vs. NCBI nr
Match: KAG6573533.1 (Thymidine kinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012648.1 Thymidine kinase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 481.5 bits (1238), Expect = 5.1e-132
Identity = 245/288 (85.07%), Postives = 263/288 (91.32%), Query Frame = 0


Query: 1   MLTISKMKSLVSSSSFSTLSPYFPITASPTFFSLPSKSTQCSPIFTQVSNYFTFKTPRIL 60
           ML ISKMKS VSSSSFSTLSPYFPITASPT  SL SKSTQC+PIFT +SN+F+ KTP + 
Sbjct: 1   MLPISKMKSFVSSSSFSTLSPYFPITASPT-LSLASKSTQCNPIFTHLSNFFSLKTPTVS 60

Query: 61  FPSKGISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIK 120
            P     S   R+ QI A+LSPPSGE+HVIVGPMFAGKTT+LLRRIQSES NGRSVAIIK
Sbjct: 61  LPRNQGFSTHKRSAQIVASLSPPSGEVHVIVGPMFAGKTTSLLRRIQSESINGRSVAIIK 120

Query: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDF 180
           SNKDTRYGLDSIVTHDGMKLPCWALPNL+SFKQKFG+GAYDKLDVIGIDEAQFFDDLYDF
Sbjct: 121 SNKDTRYGLDSIVTHDGMKLPCWALPNLSSFKQKFGEGAYDKLDVIGIDEAQFFDDLYDF 180

Query: 181 CREAADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKT 240
           CREAAD+DGKTVIVAGLDGDYLRR+FGSVL+IIPLADSVTKLTARCEICGNRAFFTLRKT
Sbjct: 181 CREAADMDGKTVIVAGLDGDYLRRSFGSVLDIIPLADSVTKLTARCEICGNRAFFTLRKT 240

Query: 241 EEKETELIGGADVYMPVCRQHYVSGQVVIETARTVV-ESHKLECRTPA 288
           EEKETELIGGAD+YMPVCRQHYVSGQVVIETARTVV E++K+E  T A
Sbjct: 241 EEKETELIGGADMYMPVCRQHYVSGQVVIETARTVVEEANKVEFTTTA 287

[Close]

BLAST of Lsi02G011250 vs. TAIR 10
Match: AT5G23070.1 (Thymidine kinase )

HSP 1 Score: 308.9 bits (790), Expect = 4.2e-84
Identity = 163/274 (59.49%), Postives = 208/274 (75.91%), Query Frame = 0


Query: 7   MKSLVSSSSFSTLSPYFPITASPTFFSLPSK-STQCSPIFTQVSNYFTFKTPRILFPSKG 66
           M++L+S S    L+P+      P+ FS   + S   + I    S   T  T ++   +  
Sbjct: 6   MRTLISPS----LAPFSLHLHKPSLFSTALRFSFSINNITPTNSPPSTISTRKLQTKATR 65

Query: 67  ISSAQNRTGQIAATLSPPSGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDT 126
           ++S+   + Q  ++ SP  GEIHV+VGPMF+GKTTTLLRRI +E   G+ +AIIKSNKDT
Sbjct: 66  VTSSS--SSQPLSSSSP--GEIHVVVGPMFSGKTTTLLRRILAERETGKRIAIIKSNKDT 125

Query: 127 RYGLDSIVTHDGMKLPCWALPNLTSFKQKFGQGAYD-KLDVIGIDEAQFFDDLYDFCREA 186
           RY  +SIVTHDG K PCW+LP+L+SFK++FG   Y+ +LDVIGIDEAQFF DLY+FCREA
Sbjct: 126 RYCTESIVTHDGEKYPCWSLPDLSSFKERFGFDDYENRLDVIGIDEAQFFGDLYEFCREA 185

Query: 187 ADIDGKTVIVAGLDGDYLRRNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKE 246
           AD +GKTVIVAGLDGD++RR FGSVL++IP+AD+VTKLT+RCE+CG RA FT+RKTEEKE
Sbjct: 186 ADKEGKTVIVAGLDGDFMRRRFGSVLDLIPIADTVTKLTSRCEVCGKRALFTMRKTEEKE 245

Query: 247 TELIGGADVYMPVCRQHYVSGQVVIETARTVVES 279
           TELIGGA+VYMPVCR HYV GQ V+ETAR V++S
Sbjct: 246 TELIGGAEVYMPVCRSHYVCGQNVLETARAVLDS 271

[Close]

BLAST of Lsi02G011250 vs. TAIR 10
Match: AT3G07800.1 (Thymidine kinase )

HSP 1 Score: 300.1 bits (767), Expect = 2.0e-81
Identity = 138/194 (71.13%), Postives = 171/194 (88.14%), Query Frame = 0


Query: 84  SGEIHVIVGPMFAGKTTTLLRRIQSESSNGRSVAIIKSNKDTRYGLDSIVTHDGMKLPCW 143
           SG +HVI+GPMF+GK+T+LLRRI+SE S+GRSVA++KS+KDTRY  DS+VTHDG+  PCW
Sbjct: 30  SGAVHVIMGPMFSGKSTSLLRRIKSEISDGRSVAMLKSSKDTRYAKDSVVTHDGIGFPCW 89

Query: 144 ALPNLTSFKQKFGQGAYDKLDVIGIDEAQFFDDLYDFCREAADIDGKTVIVAGLDGDYLR 203
           ALP+L SF +KFG  AY+KLDVIGIDEAQFF DLY+FC + AD DGK VIVAGLDGDYLR
Sbjct: 90  ALPDLMSFPEKFGLDAYNKLDVIGIDEAQFFGDLYEFCCKVADDDGKIVIVAGLDGDYLR 149

Query: 204 RNFGSVLNIIPLADSVTKLTARCEICGNRAFFTLRKTEEKETELIGGADVYMPVCRQHYV 263
           R+FG+VL+IIP+ADSVTKLTARCE+CG++AFFTLRK  +  TELIGGADVYMPVCR+HY+
Sbjct: 150 RSFGAVLDIIPIADSVTKLTARCEVCGHKAFFTLRKNCDTRTELIGGADVYMPVCRKHYI 209

Query: 264 SGQVVIETARTVVE 278
           +  +VI+ ++ V+E
Sbjct: 210 TNHIVIKASKKVLE 223

The following BLAST results are available for this feature:

BLAST of Lsi02G011250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81263	1.7e-85	69.60	Thymidine kinase OS=Oryza sativa subsp. japonica OX=39947 GN=TK PE=2 SV=2	[more]
F4KBF5	5.9e-83	59.49	Thymidine kinase b OS=Arabidopsis thaliana OX=3702 GN=TK1B PE=1 SV=1	[more]
Q9S750	2.8e-80	71.13	Thymidine kinase a OS=Arabidopsis thaliana OX=3702 GN=TK1A PE=1 SV=1	[more]
P23335	2.0e-38	45.00	Thymidine kinase OS=Swinepox virus (strain Kasza) OX=10277 GN=TK PE=3 SV=1	[more]
P04184	3.5e-35	48.11	Thymidine kinase, cytosolic OS=Mus musculus OX=10090 GN=Tk1 PE=1 SV=3	[more]

BLAST of Lsi02G011250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E495	2.7e-139	88.61	Thymidine kinase OS=Cucumis melo OX=3656 GN=LOC103501719 PE=3 SV=1	[more]
A0A0A0LYL4	5.1e-138	87.02	Thymidine kinase OS=Cucumis sativus OX=3659 GN=Csa_1G181480 PE=3 SV=1	[more]
A0A6J1HQ68	1.7e-133	85.42	Thymidine kinase OS=Cucurbita maxima OX=3661 GN=LOC111466371 PE=3 SV=1	[more]
A0A6J1EBD4	6.5e-133	85.42	Thymidine kinase OS=Cucurbita moschata OX=3662 GN=LOC111432595 PE=3 SV=1	[more]
A0A6J1CKS9	5.5e-132	83.79	Thymidine kinase OS=Momordica charantia OX=3673 GN=LOC111012527 PE=3 SV=1	[more]

BLAST of Lsi02G011250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893570.1	8.7e-148	92.68	thymidine kinase a-like [Benincasa hispida]	[more]
XP_016903056.1	5.6e-139	88.61	PREDICTED: thymidine kinase a-like [Cucumis melo]	[more]
XP_022966756.1	3.5e-133	85.42	thymidine kinase a-like [Cucurbita maxima]	[more]
XP_022925302.1	1.3e-132	85.42	thymidine kinase a-like [Cucurbita moschata]	[more]
KAG6573533.1	5.1e-132	85.07	Thymidine kinase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012648.1 ...	[more]

BLAST of Lsi02G011250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G23070.1	4.2e-84	59.49	Thymidine kinase	[more]
AT3G07800.1	2.0e-81	71.13	Thymidine kinase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.60.20		coord: 223..264 e-value: 9.0E-18 score: 66.3
None	No IPR available	PANTHER	PTHR11441:SF8	THYMIDINE KINASE B	coord: 77..283
None	No IPR available	SUPERFAMILY	57716	Glucocorticoid receptor-like (DNA-binding domain)	coord: 223..265
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 80..222 e-value: 2.1E-47 score: 162.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 85..223
IPR001267	Thymidine kinase	PFAM	PF00265	TK	coord: 85..262 e-value: 3.7E-55 score: 186.5
IPR001267	Thymidine kinase	PANTHER	PTHR11441	THYMIDINE KINASE	coord: 77..283
IPR020633	Thymidine kinase, conserved site	PROSITE	PS00603	TK_CELLULAR_TYPE	coord: 249..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi02G011250.1	Lsi02G011250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009157	deoxyribonucleoside monophosphate biosynthetic process
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004797	thymidine kinase activity