Lcy13g002410 (gene) Sponge gourd (P93075) v1

Overview
NameLcy13g002410
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr13: 2122098 .. 2122763 (+)
RNA-Seq ExpressionLcy13g002410
SyntenyLcy13g002410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGAAGGTTAACGTAGATGCAGCTTGGACTTCTTCTCCTCCGTCGACAGGGTTGGGGGCAATTAAAAGAGATTCCATGGGCAACATAGCTGGAGCTTCGGCGTTTTCACTTAAAATCGACTATAAAGCCCCCATAGCAGAAGTGATGGCAATTTTCGAACGTGTATCGCTGGCTAGGTCGCTGGGATGTAAGGAAATTATTGTGGAATTTAATTGCTCTCAGACGATTAATCTGGTGGCAAAGAAAACTGAAGTTTGGGGTGAGGTGGAGCCTTGGTTGGAGGCCATCTGGGATCAACTTGTTTTTTTCTCCAATGTCAATTTTGTATACATTTCTAGAATGAAAAATGGAGTGGCTGATGGTATAGCTAAGAGGGCTAGAATGCTTAATACATGTGAAACTTGGGTAGAAAATATCCCGAGTTGGTTGAGTTCGATGGTCGATATTGACCGTTCAGATTTTGCCCAAGTGGCGTAATAAAATTTAGTATTTTCCCAAAAAAAAGAAATAAAAAATTTGAATTCACACTTAAAGAAAAAAAAAATCAAAAGAAGATTACAAGCCATTTTTTCAATGAAAACAGAGAGGGGTTTAATTTGGACCTCAAACTTAGTTTTAGCCTTTTTTAGATTTTTTTTTTTTTTTTAGCAAGGAATTACGTAA

mRNA sequence

ATGTGGAAGGTTAACGTAGATGCAGCTTGGACTTCTTCTCCTCCGTCGACAGGGTTGGGGGCAATTAAAAGAGATTCCATGGGCAACATAGCTGGAGCTTCGGCGTTTTCACTTAAAATCGACTATAAAGCCCCCATAGCAGAAGTGATGGCAATTTTCGAACGTGTATCGCTGGCTAGGTCGCTGGGATGTAAGGAAATTATTGTGGAATTTAATTGCTCTCAGACGATTAATCTGGTGGCAAAGAAAACTGAAGTTTGGGGTGAGGTGGAGCCTTGGTTGGAGGCCATCTGGGATCAACTTCAAGGAATTACGTAA

Coding sequence (CDS)

ATGTGGAAGGTTAACGTAGATGCAGCTTGGACTTCTTCTCCTCCGTCGACAGGGTTGGGGGCAATTAAAAGAGATTCCATGGGCAACATAGCTGGAGCTTCGGCGTTTTCACTTAAAATCGACTATAAAGCCCCCATAGCAGAAGTGATGGCAATTTTCGAACGTGTATCGCTGGCTAGGTCGCTGGGATGTAAGGAAATTATTGTGGAATTTAATTGCTCTCAGACGATTAATCTGGTGGCAAAGAAAACTGAAGTTTGGGGTGAGGTGGAGCCTTGGTTGGAGGCCATCTGGGATCAACTTCAAGGAATTACGTAA

Protein sequence

MWKVNVDAAWTSSPPSTGLGAIKRDSMGNIAGASAFSLKIDYKAPIAEVMAIFERVSLARSLGCKEIIVEFNCSQTINLVAKKTEVWGEVEPWLEAIWDQLQGIT
Homology
BLAST of Lcy13g002410 vs. ExPASy TrEMBL
Match: A2ZKD1 (RNase H domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_38284 PE=4 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.7e-04
Identity = 30/88 (34.09%), Postives = 50/88 (56.82%), Query Frame = 0

Query: 3   KVNVDAAWTSSPPSTGLGAIKRDSMGNIAGASAFSLKIDYKAPIAEVMAIFERVSLARSL 62
           K+NVD ++ +S    G+GA+ RDS GN+  A+  S+ +   A  AE++A  E +++A   
Sbjct: 162 KLNVDGSFQASDGKGGIGAVLRDSSGNVIFAACGSMLVCGSAMEAELLACKEGIAMALQW 221

Query: 63  GCKEIIVEFNCSQTINLVAKKTEVWGEV 91
                IVE +C +  NL+  K +V  E+
Sbjct: 222 TFLPFIVETDCLEVFNLIQSKEKVLSEL 249

BLAST of Lcy13g002410 vs. ExPASy TrEMBL
Match: A0A6P5YMX5 (uncharacterized protein LOC111293393 OS=Durio zibethinus OX=66656 GN=LOC111293393 PE=4 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.7e-04
Identity = 29/90 (32.22%), Postives = 46/90 (51.11%), Query Frame = 0

Query: 3   KVNVDAAWTSSPPSTGLGAIKRDSMGNIAGASAFSLKIDYKAPIAEVMAIFERVSLARSL 62
           KVN D A  S      +G + RD  G +AGA AF + +   A + E +A  + +  A  +
Sbjct: 14  KVNFDGALYSHSRKGDVGVLLRDHRGQVAGAVAFIVNLVSDACVIEAIAAVKALEFATQM 73

Query: 63  GCKEIIVEFNCSQTINLVAKKTEVWGEVEP 93
           GC++I++E +     N + KK EV   + P
Sbjct: 74  GCRKIVLEGDSRIVSNCLTKKDEVLSNIGP 103

BLAST of Lcy13g002410 vs. ExPASy TrEMBL
Match: A0A6P5Y5S1 (uncharacterized protein LOC111289202 OS=Durio zibethinus OX=66656 GN=LOC111289202 PE=4 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.7e-04
Identity = 29/90 (32.22%), Postives = 46/90 (51.11%), Query Frame = 0

Query: 3   KVNVDAAWTSSPPSTGLGAIKRDSMGNIAGASAFSLKIDYKAPIAEVMAIFERVSLARSL 62
           KVN D A  S      +G + RD  G +AGA AF + +   A + E +A  + +  A  +
Sbjct: 14  KVNFDGALYSHSRKGDVGVLLRDHRGQVAGAVAFIVNLVSDACVIEAIAAVKALEFATQM 73

Query: 63  GCKEIIVEFNCSQTINLVAKKTEVWGEVEP 93
           GC++I++E +     N + KK EV   + P
Sbjct: 74  GCRKIVLEGDSRIVSNCLTKKDEVLSNIGP 103

BLAST of Lcy13g002410 vs. ExPASy TrEMBL
Match: A0A2K2CVF0 (RNase H domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_3g05652v3 PE=4 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 7.9e-04
Identity = 25/83 (30.12%), Postives = 45/83 (54.22%), Query Frame = 0

Query: 1   MWKVNVDAAWTSSPPSTGLGAIKRDSMGNIAGASAFSLKIDYKAPIAEVMAIFERVSLAR 60
           M K+NVD ++T        GA+ RD+ G   GAS   + I Y A  AE +A++  + LA+
Sbjct: 127 MQKLNVDTSFTEDMDEGATGAVIRDASGMFVGASNSFIPIVYDAATAEALALWHGIQLAK 186

Query: 61  SLGCKEIIVEFNCSQTINLVAKK 84
           + GC  +++  +  + ++ +  K
Sbjct: 187 NFGCSNLLINSDIIEVVHEMTSK 209

BLAST of Lcy13g002410 vs. NCBI nr
Match: XP_038902513.1 (uncharacterized protein LOC120089172 [Benincasa hispida])

HSP 1 Score: 70.5 bits (171), Expect = 9.9e-09
Identity = 37/97 (38.14%), Postives = 53/97 (54.64%), Query Frame = 0

Query: 3   KVNVDAAWTSSPPSTGLGAIKRDSMGNIAGASAFSLKIDYKAPIAEVMAIFERVSLARSL 62
           K+NVDAAW  SP S+G  AI RD+ G++      S+   Y  P+AE   + + + L   +
Sbjct: 65  KLNVDAAWKFSPYSSGFSAIIRDNQGSLKVVQISSIDAVYPPPLAEAFVVLQGLRLTSKM 124

Query: 63  GCKEIIVEFNCSQTINLVAKKTEVWGEVEPWLEAIWD 100
             K+IIV+ +CS  I+L  K       V  WLE IW+
Sbjct: 125 NFKKIIVKSDCSGAIDLFLKILISDSSVRVWLEEIWE 161

BLAST of Lcy13g002410 vs. NCBI nr
Match: XP_010686140.1 (PREDICTED: uncharacterized protein LOC104900422 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 59.3 bits (142), Expect = 2.3e-05
Identity = 28/81 (34.57%), Postives = 51/81 (62.96%), Query Frame = 0

Query: 3   KVNVDAAWTSSPPSTGLGAIKRDSMGNIAGASAFSLKIDYKAPIAEVMAIFERVSLARSL 62
           K+NVDA       S GLG + RD+ G+I   +A  ++ +++ PIAE  A+ E V +A++L
Sbjct: 173 KINVDAGQAGGGLS-GLGVVCRDTEGSILSCAAVQIRANWETPIAEARAVLEGVVMAKTL 232

Query: 63  GCKEIIVEFNCSQTINLVAKK 84
           G +++I+E +C + +  + K+
Sbjct: 233 GLQKVIIESDCQRVVQALQKQ 252

BLAST of Lcy13g002410 vs. NCBI nr
Match: XP_010682311.1 (PREDICTED: uncharacterized protein LOC104897185 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 58.2 bits (139), Expect = 5.1e-05
Identity = 29/82 (35.37%), Postives = 49/82 (59.76%), Query Frame = 0

Query: 3   KVNVDAAWTSSPPSTGLGAIKRDSMGNIAGASAFSLKIDYKAPIAEVMAIFERVSLARSL 62
           K NVDA   +     G+GA+ RD +G + G +     + ++  +AE  A+FE V LA++L
Sbjct: 196 KCNVDAGMVAD-FGVGVGAVCRDEVGEVLGCAVLQQHVGWEMRVAEARAVFEGVRLAKNL 255

Query: 63  GCKEIIVEFNCSQTINLVAKKT 85
           G +++IVE +C Q I+ +  K+
Sbjct: 256 GVRKLIVESDCLQVISALKSKS 276

BLAST of Lcy13g002410 vs. NCBI nr
Match: XP_018498111.1 (PREDICTED: uncharacterized protein LOC108865556 [Pyrus x bretschneideri])

HSP 1 Score: 57.4 bits (137), Expect = 8.7e-05
Identity = 28/83 (33.73%), Postives = 48/83 (57.83%), Query Frame = 0

Query: 1   MWKVNVDAAWTSSPPSTGLGAIKRDSMGNIAGASAFSLKIDYKAPIAEVMAIFERVSLAR 60
           M KVN+D +W  +P + G+G + RD +G++ G +   L I       EV AI E + LA 
Sbjct: 30  MIKVNIDGSWKGNPLAAGIGVVMRDLVGSLIGGNCIHL-IRSSPKEVEVEAILEGLKLAA 89

Query: 61  SLGCKEIIVEFNCSQTINLVAKK 84
           S G + ++VE +C   ++++ K+
Sbjct: 90  SSGHRNVVVENDCKNVMDIIQKR 111

BLAST of Lcy13g002410 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 56.2 bits (134), Expect = 1.9e-04
Identity = 34/102 (33.33%), Postives = 54/102 (52.94%), Query Frame = 0

Query: 1    MWKVNVDAAWTSSPPSTGLGAIKRDSMGNIAGASAFSLKIDYKAPIAEVMAIFERVSLAR 60
            ++KVNVDAA+ S   S G+GA+ RDS G I  A      +   A +AE  A+   + LAR
Sbjct: 1241 VFKVNVDAAFNSKNLSAGVGAVIRDSNGKIVAAGVNQNLLKGSASLAEAEAVLWGLQLAR 1300

Query: 61   SLGCKEIIVEFNCSQTINLVAKKTEVWGEVEPWLEAIWDQLQ 103
            +     +I+E +C + + LV        E+   + AI +Q++
Sbjct: 1301 NADVSSLIIESDCLEVVQLVNNTKGSRSEIFWTILAIQNQMK 1342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A2ZKD14.7e-0434.09RNase H domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_... [more]
A0A6P5YMX54.7e-0432.22uncharacterized protein LOC111293393 OS=Durio zibethinus OX=66656 GN=LOC11129339... [more]
A0A6P5Y5S14.7e-0432.22uncharacterized protein LOC111289202 OS=Durio zibethinus OX=66656 GN=LOC11128920... [more]
A0A2K2CVF07.9e-0430.12RNase H domain-containing protein OS=Brachypodium distachyon OX=15368 GN=BRADI_3... [more]
Match NameE-valueIdentityDescription
XP_038902513.19.9e-0938.14uncharacterized protein LOC120089172 [Benincasa hispida][more]
XP_010686140.12.3e-0534.57PREDICTED: uncharacterized protein LOC104900422 [Beta vulgaris subsp. vulgaris][more]
XP_010682311.15.1e-0535.37PREDICTED: uncharacterized protein LOC104897185 [Beta vulgaris subsp. vulgaris][more]
XP_018498111.18.7e-0533.73PREDICTED: uncharacterized protein LOC108865556 [Pyrus x bretschneideri][more]
XP_024950112.11.9e-0433.33uncharacterized protein LOC112496847 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 5..99
e-value: 7.0E-11
score: 42.0
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..105
e-value: 3.2E-6
score: 29.2
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 4..104
e-value: 5.28369E-12
score: 55.7832
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 1..87

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy13g002410.1Lcy13g002410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity