Lcy06g004250 (gene) Sponge gourd (P93075) v1

Overview
NameLcy06g004250
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionHigh-affinity nitrate transporter
LocationChr06: 3868459 .. 3869727 (+)
RNA-Seq ExpressionLcy06g004250
SyntenyLcy06g004250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCAAATTTTGCCTGATTCTGATTATTTAAAGAATATTCATTGTTTTAACAAAGTACTTCCTCAAATTCCCTCTTCATCATCCCAACTTGGGCTTCTCTTTGACTTTTTCTGTCACCACTTGTATATAAACCAAACCAAGGCCATAGTTCTGTTATCAATTTCATCATCATCACCATCATCAAATCTGAGATTCACTTCACTTTTTGCTGAAACTTGAGAGGTTGTTAAATCAACATGGCTGCATATGGGTTTCTCTTGAATGCTCTGTTTCTGTCCTGTTTCTTAGGATTCTCTCATGGAAATGTCCATCTTTCATCTCTCCCAAAAAGTTTGGAGGTCACTGCTTCACCAAAACCTGGCCAAGGTAAGACTTTTCAGCTCCCCCATCTCTCAAAACTCAAAACTTTGACTCTTCCATGAGAGATCTGAATCAAAAACTCAAACTTTACCAATTTCTCACAACTGGGTTGCCTTTTTTTTCTGCAGTTCTCAAAGCAGGTGTTGATAAAATCACTGTGACTTGGGTTCTAAACAGGACTGTCAAAGCCGGGTCGGATTCGGCTTACAAAACCGTCAAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAGGTGGACCGCCTATGGAGGAAAACAGAGGACAATCTCTCAAAGGACAAGACTTGCCAGTTCAACATCGTCGGAAAGCCGTACAATCCGGCGAACAAGACGGTTCAAAGCTTCGAGTGGACGGTGACGCGTGAAATTCCGACGGGGACTTTCTTCGTACGCGCCTACGCTTTCAATTCCGCCGGCGACGAAGTGGCGTACGGCCAAACGACCGACGATAAAAAGGGCACAAACTTGTTCCAAATCGAATCGATCACTGGTCGCCATGTCTCCCTCGATATCGCCTCTGTTTGTTTCAGTGCTTTCTCTGTGTTGTCTTTGTTCGGATTCTTCATCGCCGACAAGAGGAAGGCGAAGAAGGCGGCGGCGGCGCAGGGGCAGGGGCAGTAAGTTAAGAAAGGTGGGAACTTTAGTCTGTTTTGAGGTTCCCTTTTCAGGTTTGAATCAATTGCGATCTGCTTGATGAAGAGTAGATTTGATTATGAGTTTTTAAGTCTTTTTGTAATTTGGGTTTTTCTTTGTACTGTAATTTTCTAGGTGAAAAAGATAAGTTGTGTTGAGAAAAGGAAGGATATGATATGAAGTGGAATATGGTGGAAAATAAAAGTGTATTAAATTGTCTAATGTTAGACGTGGAAAATTTAAATGATAATATTTGCATCTT

mRNA sequence

CCAAATTTTGCCTGATTCTGATTATTTAAAGAATATTCATTGTTTTAACAAAGTACTTCCTCAAATTCCCTCTTCATCATCCCAACTTGGGCTTCTCTTTGACTTTTTCTGTCACCACTTGTATATAAACCAAACCAAGGCCATAGTTCTGTTATCAATTTCATCATCATCACCATCATCAAATCTGAGATTCACTTCACTTTTTGCTGAAACTTGAGAGGTTGTTAAATCAACATGGCTGCATATGGGTTTCTCTTGAATGCTCTGTTTCTGTCCTGTTTCTTAGGATTCTCTCATGGAAATGTCCATCTTTCATCTCTCCCAAAAAGTTTGGAGGTCACTGCTTCACCAAAACCTGGCCAAGTTCTCAAAGCAGGTGTTGATAAAATCACTGTGACTTGGGTTCTAAACAGGACTGTCAAAGCCGGGTCGGATTCGGCTTACAAAACCGTCAAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAGGTGGACCGCCTATGGAGGAAAACAGAGGACAATCTCTCAAAGGACAAGACTTGCCAGTTCAACATCGTCGGAAAGCCGTACAATCCGGCGAACAAGACGGTTCAAAGCTTCGAGTGGACGGTGACGCGTGAAATTCCGACGGGGACTTTCTTCGTACGCGCCTACGCTTTCAATTCCGCCGGCGACGAAGTGGCGTACGGCCAAACGACCGACGATAAAAAGGGCACAAACTTGTTCCAAATCGAATCGATCACTGGTCGCCATGTCTCCCTCGATATCGCCTCTGTTTGTTTCAGTGCTTTCTCTGTGTTGTCTTTGTTCGGATTCTTCATCGCCGACAAGAGGAAGGCGAAGAAGGCGGCGGCGGCGCAGGGGCAGGGGCAGTAAGTTAAGAAAGGTGGGAACTTTAGTCTGTTTTGAGGTTCCCTTTTCAGGTTTGAATCAATTGCGATCTGCTTGATGAAGAGTAGATTTGATTATGAGTTTTTAAGTCTTTTTGTAATTTGGGTTTTTCTTTGTACTGTAATTTTCTAGGTGAAAAAGATAAGTTGTGTTGAGAAAAGGAAGGATATGATATGAAGTGGAATATGGTGGAAAATAAAAGTGTATTAAATTGTCTAATGTTAGACGTGGAAAATTTAAATGATAATATTTGCATCTT

Coding sequence (CDS)

ATGGCTGCATATGGGTTTCTCTTGAATGCTCTGTTTCTGTCCTGTTTCTTAGGATTCTCTCATGGAAATGTCCATCTTTCATCTCTCCCAAAAAGTTTGGAGGTCACTGCTTCACCAAAACCTGGCCAAGTTCTCAAAGCAGGTGTTGATAAAATCACTGTGACTTGGGTTCTAAACAGGACTGTCAAAGCCGGGTCGGATTCGGCTTACAAAACCGTCAAGGCGAAGCTCTGCTACGCGCCGGTGAGCCAGGTGGACCGCCTATGGAGGAAAACAGAGGACAATCTCTCAAAGGACAAGACTTGCCAGTTCAACATCGTCGGAAAGCCGTACAATCCGGCGAACAAGACGGTTCAAAGCTTCGAGTGGACGGTGACGCGTGAAATTCCGACGGGGACTTTCTTCGTACGCGCCTACGCTTTCAATTCCGCCGGCGACGAAGTGGCGTACGGCCAAACGACCGACGATAAAAAGGGCACAAACTTGTTCCAAATCGAATCGATCACTGGTCGCCATGTCTCCCTCGATATCGCCTCTGTTTGTTTCAGTGCTTTCTCTGTGTTGTCTTTGTTCGGATTCTTCATCGCCGACAAGAGGAAGGCGAAGAAGGCGGCGGCGGCGCAGGGGCAGGGGCAGTAA

Protein sequence

MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNRTVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQSFEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASVCFSAFSVLSLFGFFIADKRKAKKAAAAQGQGQ
Homology
BLAST of Lcy06g004250 vs. ExPASy Swiss-Prot
Match: Q9FGS5 (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.1e-49
Identity = 109/209 (52.15%), Postives = 142/209 (67.94%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHG--NVHL-SSLPK-SLEVTASPK---PGQVLKAGVDKIT 60
           MA    L  +L +   +   HG   V L   L K +L+VT  P    PG VL AG D + 
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNRTVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNP 120
           +TW L+ ++ +  ++ +K +K KLCYAP SQVDR WRKT D L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQSFEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHV 180
            +KT+QS  WT+ R+IPTGT+FVRAYA ++ G EVAYGQ+TDD K TNLF +++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVLSLFGFFIADKRKAK 203
           SLDIAS+CFS FSV++L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

BLAST of Lcy06g004250 vs. ExPASy Swiss-Prot
Match: Q9SB67 (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 1.6e-48
Identity = 104/207 (50.24%), Postives = 140/207 (67.63%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVH---LSSLPKSLEVTASP-KPGQVLKAGVDKITVTW 60
           MA +  L  +L +   +  S G       + L  S+EVTA P K   VL+AG D +T+TW
Sbjct: 1   MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 60

Query: 61  VL-NRTVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPAN 120
            L + + K  +D+A+KT++ KLCYAP+SQVDR WRKT++ L KD++C   IV K Y   +
Sbjct: 61  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---D 120

Query: 121 KTVQSFEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSL 180
           KT QS +WT+  +IPTGT+FVRAY  +  G EVAYGQ+TD+ + TNLF + +I+G HV L
Sbjct: 121 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 180

Query: 181 DIASVCFSAFSVLSLFGFFIADKRKAK 203
           DIAS  FS FSV+SLF FF+ +KRKAK
Sbjct: 181 DIASTFFSVFSVVSLFVFFVMEKRKAK 204

BLAST of Lcy06g004250 vs. ExPASy Swiss-Prot
Match: Q6ZI50 (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.3e-42
Identity = 89/180 (49.44%), Postives = 120/180 (66.67%), Query Frame = 0

Query: 24  VHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNRTVKAGSDSAYKTVKAKLCYAPVS 83
           V LS LPK+L V  SPK G+V+ AG + +TVTW LN +  AG+D+A+K+VK KLCYAP S
Sbjct: 34  VFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPAS 93

Query: 84  QVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQSFEWTVTREIPTGTFFVRAYAFNS 143
           + DR WRK  D+L KDK CQF +  +PY         F++ V R+IPT ++FVRAYA ++
Sbjct: 94  RTDRGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDA 153

Query: 144 AGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASVCFSAFSVLSLFGFFIADKRKAKK 203
           +G EVAYGQ++ D      F +  ITG H SL +A+  FS FS+ +L  FF+ +KRK  K
Sbjct: 154 SGTEVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

BLAST of Lcy06g004250 vs. ExPASy Swiss-Prot
Match: Q7XK12 (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.1e-40
Identity = 98/218 (44.95%), Postives = 130/218 (59.63%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCF-----LGFSH-------GNVHLSSLPKSLEVTASPKPGQVLKAG 60
           MA +G +++ +FL        LG  H           LS+L K+L V ASP+ GQVL AG
Sbjct: 1   MARFGAVIHRVFLPLLLLLVVLGACHVTPAAAAAGARLSALAKALVVEASPRAGQVLHAG 60

Query: 61  VDKITVTWVLNRT---VKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFN 120
            D ITVTW LN T     AG+D+ YK VK  LCYAP SQV R WRK  D+LSKDK CQF 
Sbjct: 61  EDAITVTWSLNATAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRKAHDDLSKDKACQFK 120

Query: 121 IVGKPYNPANKTVQSFEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQI 180
           I  +PY+ A K    FE+TV R++PT +++VRAYA +++G  VAYG+T         F +
Sbjct: 121 IAQQPYDGAGK----FEYTVARDVPTASYYVRAYALDASGARVAYGETAPSAS----FAV 180

Query: 181 ESITGRHVSLDIASVCFSAFSVLSLFGFFIADKRKAKK 204
             ITG   S+++A+   SAFSV +L  F + + +K  K
Sbjct: 181 AGITGVTASIEVAAGVLSAFSVAALAVFLVLENKKKNK 210

BLAST of Lcy06g004250 vs. ExPASy TrEMBL
Match: A0A5A7ULV7 (High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004000 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.3e-97
Identity = 181/207 (87.44%), Postives = 192/207 (92.75%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNR 60
           MA+ GFLL ALFLSCFLGFS GN+HLSSLP++LEVTASPKPGQVLKAGVDKI VTWVLN 
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQS 120
           TVKAGSDS+YK +KAKLCYAPVSQVDR WRKTED+L KDKTCQF+IV KPYNPANKT QS
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV R++PTGTFFVRAY FNSAG EVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVLSLFGFFIADKRKAKKAAAA 208
           CFSAFSV+SLFGFF  DKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

BLAST of Lcy06g004250 vs. ExPASy TrEMBL
Match: A0A1S3AXC1 (High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 3.3e-97
Identity = 181/207 (87.44%), Postives = 192/207 (92.75%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNR 60
           MA+ GFLL ALFLSCFLGFS GN+HLSSLP++LEVTASPKPGQVLKAGVDKI VTWVLN 
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQS 120
           TVKAGSDS+YK +KAKLCYAPVSQVDR WRKTED+L KDKTCQF+IV KPYNPANKT QS
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV R++PTGTFFVRAY FNSAG EVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVLSLFGFFIADKRKAKKAAAA 208
           CFSAFSV+SLFGFF  DKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

BLAST of Lcy06g004250 vs. ExPASy TrEMBL
Match: A0A6J1EDS1 (High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 6.1e-96
Identity = 178/206 (86.41%), Postives = 192/206 (93.20%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNR 60
           M+A GFLL ALFL CFLGFSHG++H SSLP+SLEVTASPKPGQVLKAGVDKI+VTWVLN 
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQS 120
           TVK+GSDS+YKTV+AKLCYAPVSQVDR WRKTED+LSKDKTCQF IVGKPYNP NKTVQ 
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 FEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV R+IPTGTFFVRAYAFNSAGDEV YGQTTD+KKGTNLFQIE+ITGRH+SLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSVLSLFGFFIADKRKAKKAAA 207
           CFSAFSVLSL GFF+A KRKAKKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212

BLAST of Lcy06g004250 vs. ExPASy TrEMBL
Match: C8CHG8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2)

HSP 1 Score: 358.6 bits (919), Expect = 1.8e-95
Identity = 177/207 (85.51%), Postives = 190/207 (91.79%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNR 60
           MA+  FLL ALFLSCFLGFSHGN+H SSLP++LEVTASPKPGQVLKAGVDKI+VTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQS 120
           TVKAGSDS+YK +KAKLCYAPVSQVDR WRKTED+L KDKTCQF+IV K YNPANKTVQS
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 FEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV R+IPTGTFFVRAY  NSAG+EVAYGQTTD KKGTNLFQIESITGRH+SLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSVLSLFGFFIADKRKAKKAAAA 208
           CFSAFSV+SLFGFF  DKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

BLAST of Lcy06g004250 vs. ExPASy TrEMBL
Match: A0A6J1KMC9 (High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.5e-94
Identity = 175/206 (84.95%), Postives = 192/206 (93.20%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNR 60
           M+A GFLL ALFL CFLGFSHG++H SSLP+SLEVTASPKPGQVLKAGVDKI+VTWVLN 
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQS 120
           TVK+GSDS+YKTV+AKLCYAPVSQVDR WRKTED+LSKDKTCQF IVGKPYNPANKTVQ 
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126

Query: 121 FEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV R+IPTGTFFVRAYAFNSAG+EV YGQT+D+KKGTNLFQI++ITGRH+SLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTSDNKKGTNLFQIKAITGRHISLDIASV 186

Query: 181 CFSAFSVLSLFGFFIADKRKAKKAAA 207
           CFSAFSVLSL GFF+A KRK KKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKTKKAEA 212

BLAST of Lcy06g004250 vs. NCBI nr
Match: XP_008439011.1 (PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa] >TYJ99190.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa])

HSP 1 Score: 364.4 bits (934), Expect = 6.7e-97
Identity = 181/207 (87.44%), Postives = 192/207 (92.75%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNR 60
           MA+ GFLL ALFLSCFLGFS GN+HLSSLP++LEVTASPKPGQVLKAGVDKI VTWVLN 
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQS 120
           TVKAGSDS+YK +KAKLCYAPVSQVDR WRKTED+L KDKTCQF+IV KPYNPANKT QS
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV R++PTGTFFVRAY FNSAG EVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVLSLFGFFIADKRKAKKAAAA 208
           CFSAFSV+SLFGFF  DKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

BLAST of Lcy06g004250 vs. NCBI nr
Match: XP_022924070.1 (high-affinity nitrate transporter 3.1 [Cucurbita moschata])

HSP 1 Score: 360.1 bits (923), Expect = 1.3e-95
Identity = 178/206 (86.41%), Postives = 192/206 (93.20%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNR 60
           M+A GFLL ALFL CFLGFSHG++H SSLP+SLEVTASPKPGQVLKAGVDKI+VTWVLN 
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQS 120
           TVK+GSDS+YKTV+AKLCYAPVSQVDR WRKTED+LSKDKTCQF IVGKPYNP NKTVQ 
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 FEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV R+IPTGTFFVRAYAFNSAGDEV YGQTTD+KKGTNLFQIE+ITGRH+SLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSVLSLFGFFIADKRKAKKAAA 207
           CFSAFSVLSL GFF+A KRKAKKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212

BLAST of Lcy06g004250 vs. NCBI nr
Match: XP_023520111.1 (high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 359.4 bits (921), Expect = 2.2e-95
Identity = 177/206 (85.92%), Postives = 192/206 (93.20%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNR 60
           M+A GFLL ALFL CF GFSHG++H SSLP+SLEVTASPKPGQVLKAGVDKI+VTW+LN 
Sbjct: 7   MSANGFLLAALFLFCFFGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWILNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQS 120
           TVK+GSDS+YKTV+AKLCYAPVSQVDR WRKTED+LSKDKTCQF IVGKPYNPANKTVQ 
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126

Query: 121 FEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV R+IPTGTFFVRAYAFNSAG+EV YGQTTD+KKGTNLFQIESITGRH+SLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIESITGRHISLDIASV 186

Query: 181 CFSAFSVLSLFGFFIADKRKAKKAAA 207
           CFSAFSVLSL GFF+A KRKAKKA A
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEA 212

BLAST of Lcy06g004250 vs. NCBI nr
Match: KAG6584111.1 (High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019702.1 High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 359.0 bits (920), Expect = 2.8e-95
Identity = 177/208 (85.10%), Postives = 193/208 (92.79%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNR 60
           M+A GFLL ALFL CFLGFSHG++H SSLP+SLEVTASPKPGQVLKAGVDKI+VTWVLN 
Sbjct: 7   MSANGFLLAALFLLCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQS 120
           TVK+GSDS+YKTV+AKLCYAPVSQVDR WRKTED+LSKDKTCQF IVGKPYNP NKTVQ 
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 FEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV R+IPTGTFFVRAYAFNSAG+EV YGQTTD+KKGTNLFQIE+ITGRH+SLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSVLSLFGFFIADKRKAKKAAAAQ 209
           CFSAFSVLSL GFF+A KRKAKKA A +
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEAPE 214

BLAST of Lcy06g004250 vs. NCBI nr
Match: NP_001267619.1 (high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus] >ACV33078.2 high-affinity nitrate transport system component [Cucumis sativus] >KGN64351.1 hypothetical protein Csa_013320 [Cucumis sativus])

HSP 1 Score: 358.6 bits (919), Expect = 3.7e-95
Identity = 177/207 (85.51%), Postives = 190/207 (91.79%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVLNR 60
           MA+  FLL ALFLSCFLGFSHGN+H SSLP++LEVTASPKPGQVLKAGVDKI+VTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  TVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQS 120
           TVKAGSDS+YK +KAKLCYAPVSQVDR WRKTED+L KDKTCQF+IV K YNPANKTVQS
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 FEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180
           FEWTV R+IPTGTFFVRAY  NSAG+EVAYGQTTD KKGTNLFQIESITGRH+SLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSVLSLFGFFIADKRKAKKAAAA 208
           CFSAFSV+SLFGFF  DKRKAKKAAAA
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAA 207

BLAST of Lcy06g004250 vs. TAIR 10
Match: AT5G50200.3 (nitrate transmembrane transporters )

HSP 1 Score: 198.0 bits (502), Expect = 7.8e-51
Identity = 109/209 (52.15%), Postives = 142/209 (67.94%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHG--NVHL-SSLPK-SLEVTASPK---PGQVLKAGVDKIT 60
           MA    L  +L +   +   HG   V L   L K +L+VT  P    PG VL AG D + 
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNRTVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNP 120
           +TW L+ ++ +  ++ +K +K KLCYAP SQVDR WRKT D L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQSFEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHV 180
            +KT+QS  WT+ R+IPTGT+FVRAYA ++ G EVAYGQ+TDD K TNLF +++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVLSLFGFFIADKRKAK 203
           SLDIAS+CFS FSV++L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

BLAST of Lcy06g004250 vs. TAIR 10
Match: AT5G50200.2 (nitrate transmembrane transporters )

HSP 1 Score: 198.0 bits (502), Expect = 7.8e-51
Identity = 109/209 (52.15%), Postives = 142/209 (67.94%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHG--NVHL-SSLPK-SLEVTASPK---PGQVLKAGVDKIT 60
           MA    L  +L +   +   HG   V L   L K +L+VT  P    PG VL AG D + 
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNRTVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNP 120
           +TW L+ ++ +  ++ +K +K KLCYAP SQVDR WRKT D L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQSFEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHV 180
            +KT+QS  WT+ R+IPTGT+FVRAYA ++ G EVAYGQ+TDD K TNLF +++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVLSLFGFFIADKRKAK 203
           SLDIAS+CFS FSV++L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

BLAST of Lcy06g004250 vs. TAIR 10
Match: AT5G50200.1 (nitrate transmembrane transporters )

HSP 1 Score: 198.0 bits (502), Expect = 7.8e-51
Identity = 109/209 (52.15%), Postives = 142/209 (67.94%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHG--NVHL-SSLPK-SLEVTASPK---PGQVLKAGVDKIT 60
           MA    L  +L +   +   HG   V L   L K +L+VT  P    PG VL AG D + 
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNRTVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNP 120
           +TW L+ ++ +  ++ +K +K KLCYAP SQVDR WRKT D L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQSFEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHV 180
            +KT+QS  WT+ R+IPTGT+FVRAYA ++ G EVAYGQ+TDD K TNLF +++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVLSLFGFFIADKRKAK 203
           SLDIAS+CFS FSV++L  FF+ +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

BLAST of Lcy06g004250 vs. TAIR 10
Match: AT4G24730.4 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 194.1 bits (492), Expect = 1.1e-49
Identity = 104/207 (50.24%), Postives = 140/207 (67.63%), Query Frame = 0

Query: 1   MAAYGFLLNALFLSCFLGFSHGNVH---LSSLPKSLEVTASP-KPGQVLKAGVDKITVTW 60
           MA +  L  +L +   +  S G       + L  S+EVTA P K   VL+AG D +T+TW
Sbjct: 23  MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 82

Query: 61  VL-NRTVKAGSDSAYKTVKAKLCYAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPAN 120
            L + + K  +D+A+KT++ KLCYAP+SQVDR WRKT++ L KD++C   IV K Y   +
Sbjct: 83  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---D 142

Query: 121 KTVQSFEWTVTREIPTGTFFVRAYAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSL 180
           KT QS +WT+  +IPTGT+FVRAY  +  G EVAYGQ+TD+ + TNLF + +I+G HV L
Sbjct: 143 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 202

Query: 181 DIASVCFSAFSVLSLFGFFIADKRKAK 203
           DIAS  FS FSV+SLF FF+ +KRKAK
Sbjct: 203 DIASTFFSVFSVVSLFVFFVMEKRKAK 226

BLAST of Lcy06g004250 vs. TAIR 10
Match: AT4G24730.1 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 189.5 bits (480), Expect = 2.8e-48
Identity = 99/184 (53.80%), Postives = 130/184 (70.65%), Query Frame = 0

Query: 20  SHGNVHLSSLPKSLEVTASPKPGQVLKAGVDKITVTWVL-NRTVKAGSDSAYKTVKAKLC 79
           SHG VH  SL  +LE         VL+AG D +T+TW L + + K  +D+A+KT++ KLC
Sbjct: 265 SHG-VHHRSLEAALECP------PVLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLC 324

Query: 80  YAPVSQVDRLWRKTEDNLSKDKTCQFNIVGKPYNPANKTVQSFEWTVTREIPTGTFFVRA 139
           YAP+SQVDR WRKT++ L KD++C   IV K Y   +KT QS +WT+  +IPTGT+FVRA
Sbjct: 325 YAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRA 384

Query: 140 YAFNSAGDEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASVCFSAFSVLSLFGFFIADK 199
           Y  +  G EVAYGQ+TD+ + TNLF + +I+G HV LDIAS  FS FSV+SLF FF+ +K
Sbjct: 385 YGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEK 438

Query: 200 RKAK 203
           RKAK
Sbjct: 445 RKAK 438

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FGS51.1e-4952.15High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 ... [more]
Q9SB671.6e-4850.24High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 ... [more]
Q6ZI501.3e-4249.44High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ... [more]
Q7XK122.1e-4044.95Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ... [more]
Match NameE-valueIdentityDescription
A0A5A7ULV73.3e-9787.44High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3AXC13.3e-9787.44High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 S... [more]
A0A6J1EDS16.1e-9686.41High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 ... [more]
C8CHG81.8e-9585.51High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2[more]
A0A6J1KMC91.5e-9484.95High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111495476 PE... [more]
Match NameE-valueIdentityDescription
XP_008439011.16.7e-9787.44PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266... [more]
XP_022924070.11.3e-9586.41high-affinity nitrate transporter 3.1 [Cucurbita moschata][more]
XP_023520111.12.2e-9585.92high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo][more]
KAG6584111.12.8e-9585.10High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. so... [more]
NP_001267619.13.7e-9585.51high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus] >ACV33078... [more]
Match NameE-valueIdentityDescription
AT5G50200.37.8e-5152.15nitrate transmembrane transporters [more]
AT5G50200.27.8e-5152.15nitrate transmembrane transporters [more]
AT5G50200.17.8e-5152.15nitrate transmembrane transporters [more]
AT4G24730.41.1e-4950.24Calcineurin-like metallo-phosphoesterase superfamily protein [more]
AT4G24730.12.8e-4853.80Calcineurin-like metallo-phosphoesterase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016605High-affinity nitrate transporterPFAMPF16974NAR2coord: 27..201
e-value: 3.6E-76
score: 254.4
IPR016605High-affinity nitrate transporterPIRSFPIRSF012939NAR2coord: 1..205
e-value: 2.5E-83
score: 276.3
IPR016605High-affinity nitrate transporterPANTHERPTHR34806HIGH-AFFINITY NITRATE TRANSPORTER 3.2coord: 6..204
NoneNo IPR availablePANTHERPTHR34806:SF1HIGH-AFFINITY NITRATE TRANSPORTER 3.2coord: 6..204

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy06g004250.1Lcy06g004250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042128 nitrate assimilation
biological_process GO:0015706 nitrate transport
biological_process GO:0010167 response to nitrate
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015112 nitrate transmembrane transporter activity