Lcy04g008360.1 (mRNA) Sponge gourd (P93075) v1

Overview
NameLcy04g008360.1
TypemRNA
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionAdenine/guanine permease
LocationChr04: 39258726 .. 39260688 (-)
Sequence length1963
RNA-Seq ExpressionLcy04g008360.1
SyntenyLcy04g008360.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCAACACACCCACTACTCTTCCTGCCCTTAAGCAACTCACCACTCAACAACCACCTCTCATGGCGGCGCCCACGACGGCAGCGCCTCTACCATCACCGCCGCAGCGGAAGCCATCTCTACCCACCAGGCTAAACTCTTTCGTCGCCTCCACCCGCCTCGGAAAGCGCTTCAAACTCGCCGAACGAAACACCACCTTCACCACCGAGCTCCGCGCCGGCACCGCCACTTTCCTCACCATGGCCTACATTCTCGCCGTCAACGCCAGCATCCTCACCGACTCCGGCGGCACGTGCACCACCGCCGACTGCACGCCCCTCTGCTCCGACCCCACCGTCCCACTAGCCGCCTGCACCGGTCCTGCCCACCACGTCATCCAACCGGACGGTTCCTGCAAATTCCGACCCGTCAACCCGGGTTACGCCGCCTGCTTGGAAACGACCCGGAAAGATCTGATCATCGCTACCGGAGCCTCGTCCCTCATCGGCAGTTTCGTAATGGGCGTTTTCGCGAATCTCCCTTTAGCCTTAGCTCCGGGAATGGGAACCAACGCTTATTTCGCCTACGCCGTAGTCGGGTTTCACGGGTCGGGTCAGCTGCCGTACAGAAGCGCATTGGCCGCCATTTTCATCGAGGGCTTAATCTTCCTTCTGATATCCGCCGTCGGATTACGAGCCCAAATCGCCAAATTAATCCCGAAACCCATCAGAATCTCGTCCTCCGCCGGAATCGGATTATTCCTCGCCTTCATCGGATTACAGAGCAACCAAGGAATTGGGCTCGTCGGGTACAGCGCCTCGACGCTCGTCACACTCGGAGCCTGCCCGTTAAGTTCCCGGGCAGCGTTAGCTGCTGTGGTAACTGCCCCGAATGGAACGGTCAGTTTGCTCCCGAACGGCGCCGTTTCGGATCAGATCATGTGCCTTAACGGCCGGATGGAAAGCCCCACGTTCTGGCTCGGCGTGGTCGGATTCGTGATCATTGCGTATTGTTTGGTGAAGGACGTGAAAGGCGGGATGATATACGGCGTCGTTTTCGTGACGGCGATTTCGTGGTTCCGGAACACGGCGGTGACGGCGTTCCCCGGCACGGCGGCCGGCGACGCTGCGTATGGGTATTTCAAGAAGGTCGTCGACGTGCATTTGATTAAAACCACCGCCGGAGCTTTGAGTTTTCGGGATCTGGGGAAGCCCCATTTCTGGGAAGCTCTGTTCACGTTCCTTTACGTCGACATTCTCGACACCACCGGAACTCTGTACTCCGCCGCCCGTTTCGCCGGATTCACCGACGCCAACGGCGACTTCGAGGGGCAGTATTTTGCCTTCATGTCGGACGCTTTTTCGATCGTGGTTGGGTCTCTGCTCGGAACCTCGCCGGTGTCGACATACATCGAGTCGTCGACGGGAATTCGGGAGGGAGGGAGAACGGGGCTGACGGCGGTGACGGTGGCAGGATACTTTCTGATGTCGTTCTGGTTTACGCCGCTGCTGGCGTCGATTCCCCCGTGGGCGGTGGGGCCGCCGCTGATTCTGGTGGGGGTTCTGATGGCGAGATCGATGGTGGAGATTGAATGGGGGGATATGAGAGAAGCAATTCCGGCATTTTTGACGATGATTTTGATGCCGTTGACATATTCGATTGCTTATGGATTGATCGGCGGGATTGGGACGTTCGTGGTGCTGCATCTATGGGACTGGTGCGGCGTCGTTTTGGAGAAGCTCCGAGCTCGTAAAAGTTGTTGTAACAGAAATAATGGTCGAAAAGAGATCCAATTGTCCAATGGAAATTCCAGTGCTTCCTCTAGGGAACGCCCAAAAACACTTCAAGTTTGATTTTTTTTTCTTCTTCCTTATTTTTGAGGCTACAGTGTATATTTTAGATGGAAAAATACTCGGCTGCATATAGGTGTAGAGATCTTCCTGTGTACGAACGTTAAAATATTGACAACTAAGAGAGATAAAAAC

mRNA sequence

CTTCAACACACCCACTACTCTTCCTGCCCTTAAGCAACTCACCACTCAACAACCACCTCTCATGGCGGCGCCCACGACGGCAGCGCCTCTACCATCACCGCCGCAGCGGAAGCCATCTCTACCCACCAGGCTAAACTCTTTCGTCGCCTCCACCCGCCTCGGAAAGCGCTTCAAACTCGCCGAACGAAACACCACCTTCACCACCGAGCTCCGCGCCGGCACCGCCACTTTCCTCACCATGGCCTACATTCTCGCCGTCAACGCCAGCATCCTCACCGACTCCGGCGGCACGTGCACCACCGCCGACTGCACGCCCCTCTGCTCCGACCCCACCGTCCCACTAGCCGCCTGCACCGGTCCTGCCCACCACGTCATCCAACCGGACGGTTCCTGCAAATTCCGACCCGTCAACCCGGGTTACGCCGCCTGCTTGGAAACGACCCGGAAAGATCTGATCATCGCTACCGGAGCCTCGTCCCTCATCGGCAGTTTCGTAATGGGCGTTTTCGCGAATCTCCCTTTAGCCTTAGCTCCGGGAATGGGAACCAACGCTTATTTCGCCTACGCCGTAGTCGGGTTTCACGGGTCGGGTCAGCTGCCGTACAGAAGCGCATTGGCCGCCATTTTCATCGAGGGCTTAATCTTCCTTCTGATATCCGCCGTCGGATTACGAGCCCAAATCGCCAAATTAATCCCGAAACCCATCAGAATCTCGTCCTCCGCCGGAATCGGATTATTCCTCGCCTTCATCGGATTACAGAGCAACCAAGGAATTGGGCTCGTCGGGTACAGCGCCTCGACGCTCGTCACACTCGGAGCCTGCCCGTTAAGTTCCCGGGCAGCGTTAGCTGCTGTGGTAACTGCCCCGAATGGAACGGTCAGTTTGCTCCCGAACGGCGCCGTTTCGGATCAGATCATGTGCCTTAACGGCCGGATGGAAAGCCCCACGTTCTGGCTCGGCGTGGTCGGATTCGTGATCATTGCGTATTGTTTGGTGAAGGACGTGAAAGGCGGGATGATATACGGCGTCGTTTTCGTGACGGCGATTTCGTGGTTCCGGAACACGGCGGTGACGGCGTTCCCCGGCACGGCGGCCGGCGACGCTGCGTATGGGTATTTCAAGAAGGTCGTCGACGTGCATTTGATTAAAACCACCGCCGGAGCTTTGAGTTTTCGGGATCTGGGGAAGCCCCATTTCTGGGAAGCTCTGTTCACGTTCCTTTACGTCGACATTCTCGACACCACCGGAACTCTGTACTCCGCCGCCCGTTTCGCCGGATTCACCGACGCCAACGGCGACTTCGAGGGGCAGTATTTTGCCTTCATGTCGGACGCTTTTTCGATCGTGGTTGGGTCTCTGCTCGGAACCTCGCCGGTGTCGACATACATCGAGTCGTCGACGGGAATTCGGGAGGGAGGGAGAACGGGGCTGACGGCGGTGACGGTGGCAGGATACTTTCTGATGTCGTTCTGGTTTACGCCGCTGCTGGCGTCGATTCCCCCGTGGGCGGTGGGGCCGCCGCTGATTCTGGTGGGGGTTCTGATGGCGAGATCGATGGTGGAGATTGAATGGGGGGATATGAGAGAAGCAATTCCGGCATTTTTGACGATGATTTTGATGCCGTTGACATATTCGATTGCTTATGGATTGATCGGCGGGATTGGGACGTTCGTGGTGCTGCATCTATGGGACTGGTGCGGCGTCGTTTTGGAGAAGCTCCGAGCTCGTAAAAGTTGTTGTAACAGAAATAATGGTCGAAAAGAGATCCAATTGTCCAATGGAAATTCCAGTGCTTCCTCTAGGGAACGCCCAAAAACACTTCAAGTTTGATTTTTTTTTCTTCTTCCTTATTTTTGAGGCTACAGTGTATATTTTAGATGGAAAAATACTCGGCTGCATATAGGTGTAGAGATCTTCCTGTGTACGAACGTTAAAATATTGACAACTAAGAGAGATAAAAAC

Coding sequence (CDS)

ATGGCGGCGCCCACGACGGCAGCGCCTCTACCATCACCGCCGCAGCGGAAGCCATCTCTACCCACCAGGCTAAACTCTTTCGTCGCCTCCACCCGCCTCGGAAAGCGCTTCAAACTCGCCGAACGAAACACCACCTTCACCACCGAGCTCCGCGCCGGCACCGCCACTTTCCTCACCATGGCCTACATTCTCGCCGTCAACGCCAGCATCCTCACCGACTCCGGCGGCACGTGCACCACCGCCGACTGCACGCCCCTCTGCTCCGACCCCACCGTCCCACTAGCCGCCTGCACCGGTCCTGCCCACCACGTCATCCAACCGGACGGTTCCTGCAAATTCCGACCCGTCAACCCGGGTTACGCCGCCTGCTTGGAAACGACCCGGAAAGATCTGATCATCGCTACCGGAGCCTCGTCCCTCATCGGCAGTTTCGTAATGGGCGTTTTCGCGAATCTCCCTTTAGCCTTAGCTCCGGGAATGGGAACCAACGCTTATTTCGCCTACGCCGTAGTCGGGTTTCACGGGTCGGGTCAGCTGCCGTACAGAAGCGCATTGGCCGCCATTTTCATCGAGGGCTTAATCTTCCTTCTGATATCCGCCGTCGGATTACGAGCCCAAATCGCCAAATTAATCCCGAAACCCATCAGAATCTCGTCCTCCGCCGGAATCGGATTATTCCTCGCCTTCATCGGATTACAGAGCAACCAAGGAATTGGGCTCGTCGGGTACAGCGCCTCGACGCTCGTCACACTCGGAGCCTGCCCGTTAAGTTCCCGGGCAGCGTTAGCTGCTGTGGTAACTGCCCCGAATGGAACGGTCAGTTTGCTCCCGAACGGCGCCGTTTCGGATCAGATCATGTGCCTTAACGGCCGGATGGAAAGCCCCACGTTCTGGCTCGGCGTGGTCGGATTCGTGATCATTGCGTATTGTTTGGTGAAGGACGTGAAAGGCGGGATGATATACGGCGTCGTTTTCGTGACGGCGATTTCGTGGTTCCGGAACACGGCGGTGACGGCGTTCCCCGGCACGGCGGCCGGCGACGCTGCGTATGGGTATTTCAAGAAGGTCGTCGACGTGCATTTGATTAAAACCACCGCCGGAGCTTTGAGTTTTCGGGATCTGGGGAAGCCCCATTTCTGGGAAGCTCTGTTCACGTTCCTTTACGTCGACATTCTCGACACCACCGGAACTCTGTACTCCGCCGCCCGTTTCGCCGGATTCACCGACGCCAACGGCGACTTCGAGGGGCAGTATTTTGCCTTCATGTCGGACGCTTTTTCGATCGTGGTTGGGTCTCTGCTCGGAACCTCGCCGGTGTCGACATACATCGAGTCGTCGACGGGAATTCGGGAGGGAGGGAGAACGGGGCTGACGGCGGTGACGGTGGCAGGATACTTTCTGATGTCGTTCTGGTTTACGCCGCTGCTGGCGTCGATTCCCCCGTGGGCGGTGGGGCCGCCGCTGATTCTGGTGGGGGTTCTGATGGCGAGATCGATGGTGGAGATTGAATGGGGGGATATGAGAGAAGCAATTCCGGCATTTTTGACGATGATTTTGATGCCGTTGACATATTCGATTGCTTATGGATTGATCGGCGGGATTGGGACGTTCGTGGTGCTGCATCTATGGGACTGGTGCGGCGTCGTTTTGGAGAAGCTCCGAGCTCGTAAAAGTTGTTGTAACAGAAATAATGGTCGAAAAGAGATCCAATTGTCCAATGGAAATTCCAGTGCTTCCTCTAGGGAACGCCCAAAAACACTTCAAGTTTGA

Protein sequence

MAAPTTAAPLPSPPQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAYILAVNASILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLETTRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALAAIFIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSASTLVTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGVVGFVIIAYCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTAGALSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFMSDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHLWDWCGVVLEKLRARKSCCNRNNGRKEIQLSNGNSSASSRERPKTLQV
Homology
BLAST of Lcy04g008360.1 vs. ExPASy Swiss-Prot
Match: Q9SRK7 (Adenine/guanine permease AZG1 OS=Arabidopsis thaliana OX=3702 GN=AZG1 PE=2 SV=1)

HSP 1 Score: 828.2 bits (2138), Expect = 6.0e-239
Identity = 420/545 (77.06%), Postives = 477/545 (87.52%), Query Frame = 0

Query: 10  LPSPPQR-KPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAYILAVNA 69
           LPS   R KP L  RLN++V S+R+GKRFKLAERN+TFTTELRAGTATFLTMAYILAVNA
Sbjct: 9   LPSTTTRPKPKLLNRLNTYVGSSRVGKRFKLAERNSTFTTELRAGTATFLTMAYILAVNA 68

Query: 70  SILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLETTR 129
           SIL+DSGGTC+ +DC PLCS+P +  + CTGP   +IQPD SCKF PVNPGYAAC+E  R
Sbjct: 69  SILSDSGGTCSVSDCIPLCSNPAIEPSQCTGPGLRLIQPDVSCKFNPVNPGYAACVEEIR 128

Query: 130 KDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALAAI 189
           KDLI+AT A+SLIG  +MG+ ANLPLALAPGMGTNAYFAY VVGFHGSG + YR+ALAA+
Sbjct: 129 KDLIVATVAASLIGCVIMGLMANLPLALAPGMGTNAYFAYTVVGFHGSGSISYRTALAAV 188

Query: 190 FIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSASTL 249
           FIEGLIFL ISA+G RA++AKL+PKP+RISSSAGIGLFLAFIGLQ+NQGIGLVGYS STL
Sbjct: 189 FIEGLIFLFISAIGFRAKLAKLVPKPVRISSSAGIGLFLAFIGLQNNQGIGLVGYSPSTL 248

Query: 250 VTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGVVGFVIIA 309
           VTL ACP SSR +LA V+T+ NGTVSLL  G+VS  IMC++GRMESPTFWLG+VGFVIIA
Sbjct: 249 VTLAACPASSRISLAPVITSANGTVSLLAGGSVSGDIMCIHGRMESPTFWLGIVGFVIIA 308

Query: 310 YCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTAGA 369
           YCLVK+VKG MIYG+VFVTA+SWFRNT VTAFP T+AGDAA+ YFKK+VDVH+IK TAGA
Sbjct: 309 YCLVKNVKGAMIYGIVFVTAVSWFRNTEVTAFPNTSAGDAAHDYFKKIVDVHVIKHTAGA 368

Query: 370 LSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFMSDAFSI 429
           LSF  + K HFWEAL TFLYVDILDTTGTLYS ARFAGF D  GDF GQYFAFMSDA +I
Sbjct: 369 LSFSGINKGHFWEALVTFLYVDILDTTGTLYSMARFAGFVDEKGDFAGQYFAFMSDASAI 428

Query: 430 VVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGPPL 489
           V+GSLLGTSPV+ +IESSTGIREGGRTGLTA+TVA YFL++ +FTPLLASIP WAVGPPL
Sbjct: 429 VIGSLLGTSPVTVFIESSTGIREGGRTGLTAITVAVYFLLAMFFTPLLASIPAWAVGPPL 488

Query: 490 ILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHLWDWCGV 549
           ILVGV+M +S+ EI+W DMREAIPAF+TMILMPLTYS+AYGLIGGIG++VVLHLWDW   
Sbjct: 489 ILVGVMMMKSVTEIDWEDMREAIPAFVTMILMPLTYSVAYGLIGGIGSYVVLHLWDWGEE 548

Query: 550 VLEKL 554
            L KL
Sbjct: 549 GLVKL 553

BLAST of Lcy04g008360.1 vs. ExPASy Swiss-Prot
Match: Q84MA8 (Adenine/guanine permease AZG2 OS=Arabidopsis thaliana OX=3702 GN=AZG2 PE=2 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 6.8e-142
Identity = 266/523 (50.86%), Postives = 351/523 (67.11%), Query Frame = 0

Query: 24  LNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAYILAVNASILTDSGGTCTTADC 83
           LN  V+ + +G+ FKL  R TTFTTELRA TATFLTMAYI+ VNA+IL DSG TC+  DC
Sbjct: 18  LNDTVSKSFIGRFFKLEARKTTFTTELRAATATFLTMAYIITVNANILADSGATCSINDC 77

Query: 84  TPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLETTRKDLIIATGASSLIGS 143
           + + S                  P G       NPGY  C+   +KDL++AT  S+++GS
Sbjct: 78  STVASS----------------SPPGPECVLGSNPGYEQCISRVKKDLVVATSLSAMVGS 137

Query: 144 FVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALAAIFIEGLIFLLISAVGL 203
             MG+ ANLP  LAPGMG NAY AY VVGF GSG + Y +A+A + +EG  FL +SA+GL
Sbjct: 138 LAMGLLANLPFGLAPGMGANAYIAYNVVGFRGSGSISYHTAMAIVLLEGCAFLAVSALGL 197

Query: 204 RAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSASTLVTLGACPLSSRAALA 263
           R ++A+LIP+ +R++ + GIG+F+AF+GLQ NQGIGLVG   STLVTL AC         
Sbjct: 198 RGKLARLIPQTVRLACAVGIGMFIAFVGLQMNQGIGLVGPDKSTLVTLTAC--------- 257

Query: 264 AVVTAPNGTVSLLPNGAVSDQI--MCLNGRMESPTFWLGVVGFVIIAYCLVKDVKGGMIY 323
                           A +D +   CL G+M+SPTFWL VVGF+I ++ L+K+VKG MIY
Sbjct: 258 ----------------AETDPVTGACLGGKMKSPTFWLAVVGFLITSFGLMKNVKGSMIY 317

Query: 324 GVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTAGALSFRDLGKPHFWE 383
           G+VFVTAISW R T VT FP T  GD+ Y YF K+VD H I++T GA+SF +  K   W 
Sbjct: 318 GIVFVTAISWIRGTQVTIFPHTPLGDSNYNYFTKIVDFHKIQSTLGAISFTEFRKSEVWV 377

Query: 384 ALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFMSDAFSIVVGSLLGTSPVST 443
           A  T  YVD+L TTG LY+ A   GF + +G FEG+Y A++ DA S VVGS LG +  +T
Sbjct: 378 AFATLFYVDLLGTTGVLYTMAEIGGFVE-DGKFEGEYAAYLVDAGSSVVGSALGVTTTAT 437

Query: 444 YIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGPPLILVGVLMARSMVE 503
           ++ESS G++EGG+TGLTAV V  YFL S +FTPL+ ++P WAVGP L++VGV+M   + +
Sbjct: 438 FVESSAGLKEGGKTGLTAVIVGLYFLASMFFTPLVTNVPRWAVGPSLVMVGVMMMGVVKD 497

Query: 504 IEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHLWD 545
           I WG+ +EA+ AF+T++LMPLTYSIA G+I GIG ++ L ++D
Sbjct: 498 IRWGETKEAVTAFVTILLMPLTYSIANGIIAGIGIYLALSMYD 498

BLAST of Lcy04g008360.1 vs. ExPASy Swiss-Prot
Match: L7WRR4 (Efflux pump notK' OS=Aspergillus versicolor OX=46472 GN=notK' PE=1 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 7.1e-99
Identity = 219/535 (40.93%), Postives = 305/535 (57.01%), Query Frame = 0

Query: 23  RLNSFVASTRLGKRFKL-------AERNTTFTTELRAGTATFLTMAYILAVNASILTDSG 82
           R N+ VA + +GK F+L         +   F TELRAG ATF  MAYI++VNA+I +D+G
Sbjct: 9   RTNTAVARSAVGKWFRLEGSGHPRERKGAYFFTELRAGLATFFAMAYIISVNANITSDTG 68

Query: 83  GTCT--TADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLETTRKDLII 142
            TC     D    C++                           N  Y  C +   +D++ 
Sbjct: 69  ATCVCPAEDLETHCNN---------------------------NTEYLLCKQEVNRDIVT 128

Query: 143 ATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALAAIFIEGL 202
           AT A + + SF +G+ ANLP+ALAPGMG NAYFAY VVG HGSG +PY  A+ A+F+EG 
Sbjct: 129 ATAAIASVASFFLGLLANLPVALAPGMGLNAYFAYTVVGHHGSGLIPYSLAVTAVFVEGW 188

Query: 203 IFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSASTLVTLGA 262
           IFL ++ +G+R  +A+ IP  I++++ AGIGL+L  IGL  + G+GLV  +  + + L  
Sbjct: 189 IFLGLTMLGIRQWLARAIPASIKLATGAGIGLYLTLIGLSYSAGLGLVQGAQDSPIQLAG 248

Query: 263 CPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGV-VGFVIIAYCLV 322
           C                       +   SD +     +M +PT W+G+  G     + ++
Sbjct: 249 C---------------------ASDEFDSDGLCPSYAKMRNPTMWIGIFCGGFFTVFLMM 308

Query: 323 KDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTAGALSFR 382
             VKG +I G++ V+ ISW R T VT FP T  GD+ + +FKKVV  H I+ T  A  + 
Sbjct: 309 YRVKGAVIAGILLVSIISWPRTTPVTYFPHTTEGDSMFDFFKKVVTFHPIQHTLVAQDWN 368

Query: 383 -DLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTD-ANGDFEGQYFAFMSDAFSIVV 442
                  F  AL TFLYVDILD TGTLYS A+FAG  D    DFEG   A+  DA  I +
Sbjct: 369 ISSNGGQFGLALITFLYVDILDATGTLYSMAKFAGAMDERTQDFEGSAMAYTVDAICISI 428

Query: 443 GSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGPPLIL 502
           GSL G+ PV+ ++ES  GI EGG+TGLT+      F ++ +F P+ ASIPPWA G  L++
Sbjct: 429 GSLFGSPPVTAFVESGAGISEGGKTGLTSCMTGICFFIAVFFAPIFASIPPWATGSTLVI 488

Query: 503 VGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHLWDW 546
           VG +M  + +EI W  M +AIPAFLT+ +MP TYSIA GLI GI ++++++   W
Sbjct: 489 VGSMMMHATLEINWRYMGDAIPAFLTISVMPFTYSIADGLIAGIISYILINGGVW 495

BLAST of Lcy04g008360.1 vs. ExPASy Swiss-Prot
Match: O94300 (Putative xanthine/uracil permease C887.17 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC887.17 PE=3 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 7.1e-99
Identity = 217/523 (41.49%), Postives = 300/523 (57.36%), Query Frame = 0

Query: 28  VASTRLGKRFKL-------AERNTTFTTELRAGTATFLTMAYILAVNASILTDSGGTCTT 87
           VA +  G+ F+L         + + F+ E+ AG  TF  MAYILAVNA+IL D+GGTC  
Sbjct: 18  VARSAFGRWFRLEGCGHPRERKGSRFSLEISAGLTTFFAMAYILAVNATILVDTGGTC-- 77

Query: 88  ADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLETTRKDLIIATGASSL 147
            +CT    D    L                         Y  C E   +DL+ AT A S 
Sbjct: 78  -ECTEANRDDCDKL-----------------------DDYVLCKEDFHRDLVTATAAISA 137

Query: 148 IGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALAAIFIEGLIFLLISA 207
           + SF MG+FAN+P+ +APGMG NAYFAY VVG++G+G++ YR AL A+F+EG IF  ++ 
Sbjct: 138 LASFCMGLFANMPVGMAPGMGLNAYFAYQVVGYNGTGRVSYREALLAVFVEGFIFTGLTV 197

Query: 208 VGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSASTLVTLGACPLSSRA 267
           +GLR  +A++IP  ++ ++ AGIGL+L  IGL  + G+G++G+S+S +V LG CP     
Sbjct: 198 IGLRQWLARVIPASLKFATGAGIGLYLTIIGLSPSAGLGVIGHSSSDIVALGGCP----- 257

Query: 268 ALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGV-VGFVIIAYCLVKDVKGGM 327
                           P    +D   C   +++S   W+G+  G V+ A  ++   KG +
Sbjct: 258 ----------------PEYLNAD-YSCNGHQLQSGRMWVGIFCGGVLTAILMMYKFKGAV 317

Query: 328 IYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTAGALSFRDLGKPHF 387
           + G+  VT  SW R + VT FP T  GD  + +FKKVV    I     A  +   G   F
Sbjct: 318 LAGIALVTITSWPRRSLVTMFPHTLTGDYNFDFFKKVVSFRKINRILVAQQWNVTG-GQF 377

Query: 388 WEALFTFLYVDILDTTGTLYSAARFAGFTD-ANGDFEGQYFAFMSDAFSIVVGSLLGTSP 447
             AL TFLYVDI+D TGTLYS A +AG  D    DFEG   A++ DA SI +GSL G SP
Sbjct: 378 AIALITFLYVDIMDMTGTLYSMANYAGLVDPRTQDFEGSAVAYIVDALSISIGSLFGCSP 437

Query: 448 VSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGPPLILVGVLMARS 507
           V+ +IES +GI  GGRTG+  + V   F +S +F P+ +SIP WA G  L+LVG +M +S
Sbjct: 438 VTAFIESGSGISAGGRTGILGMVVGICFFISLFFAPIFSSIPVWATGSTLVLVGSMMMKS 491

Query: 508 MVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLH 542
              I W  + ++IPAF+T+ LMP TYSIAYGLI GI  + +L+
Sbjct: 498 TTLINWSYLGDSIPAFITIALMPFTYSIAYGLIAGIICYALLN 491

BLAST of Lcy04g008360.1 vs. ExPASy Swiss-Prot
Match: Q57772 (Putative permease MJ0326 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0326 PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.4e-56
Identity = 167/509 (32.81%), Postives = 250/509 (49.12%), Query Frame = 0

Query: 35  KRFKLAERNTTFTTELRAGTATFLTMAYILAVNASILTDSGGTCTTADCTPLCSDPTVPL 94
           K F+  +  T    E  AG  TF+TMAYI+ VN  IL+ +G                   
Sbjct: 8   KYFEFEKYGTNLKVETLAGITTFMTMAYIIFVNPQILSTAG------------------- 67

Query: 95  AACTGPAHHVIQPDGSCKFRPVNPGYAACLETTRKDLIIATGASSLIGSFVMGVFANLPL 154
                                    + A        +++AT  +S I + VMG++A  P 
Sbjct: 68  -----------------------MDFGA--------VMVATCIASAIATLVMGLYARYPF 127

Query: 155 ALAPGMGTNAYFAYAVVGFHGSGQLPYRSALAAIFIEGLIFLLISAVGLRAQIAKLIPKP 214
           ALAPGMG NAYF Y V    G G + +R AL A+FI G++F++++   +R  I  +IP  
Sbjct: 128 ALAPGMGLNAYFTYGVC--LGMG-IDWRVALGAVFISGVLFIILTLTKIRTWIFNVIPNA 187

Query: 215 IRISSSAGIGLFLAFIGLQSNQGIGLVGYSASTLVTLGACPLSSRAALAAVVTAPNGTVS 274
           I+  ++ GIGLF+AFIGL+S    G++  S +TLVTL                       
Sbjct: 188 IKYGTAVGIGLFIAFIGLKS---AGIIVSSKATLVTL----------------------- 247

Query: 275 LLPNGAVSDQIMCLNGRMESPTFWLGVVGFVIIAYCLVKDVKGGMIYGVVFVTAISWFRN 334
                          G +  P+  L + G  + +  + ++V G ++ G++  + I     
Sbjct: 248 ---------------GNLMEPSTLLALFGIFLTSILVSRNVIGAILIGIIVTSLIGMI-- 307

Query: 335 TAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTAGALSFRDLGKPHFWEALFTFLYVDILDT 394
             ++ FP     +  +     +    L     GAL+   L        +  F +VD+ DT
Sbjct: 308 LGISPFP-----EGIFSMPPSIAPTFLQLDIMGALNLGLL------TIVLAFFFVDMFDT 367

Query: 395 TGTLYSAARFAGFTDANGDFEGQYFAFMSDAFSIVVGSLLGTSPVSTYIESSTGIREGGR 454
            GTL + A  AG+ D +G       A M+DA   VVGSLLGTS V+TYIES++GI  GGR
Sbjct: 368 LGTLSALASQAGYLDKDGKLPRVEKALMADATGTVVGSLLGTSTVTTYIESASGIALGGR 409

Query: 455 TGLTAVTVAGYFLMSFWFTPLLASIPPWAVGPPLILVGVLMARSMVEIEWGDMREAIPAF 514
           TG  +V VA  FL+S +F P++ +IPP+A    L++VG LM RS+  I++ D  EAIPAF
Sbjct: 428 TGFVSVVVAMLFLLSLFFYPVVKAIPPYATAAALVIVGALMMRSVKYIDFDDYTEAIPAF 409

Query: 515 LTMILMPLTYSIAYGLIGGIGTFVVLHLW 544
           +T++ +PLT+SIA GL  G  T+ +L ++
Sbjct: 488 ITLLTIPLTFSIATGLALGFITYPILKVF 409

BLAST of Lcy04g008360.1 vs. ExPASy TrEMBL
Match: A0A6J1HT63 (adenine/guanine permease AZG1-like OS=Cucurbita maxima OX=3661 GN=LOC111465968 PE=4 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 1.3e-297
Identity = 528/583 (90.57%), Postives = 555/583 (95.20%), Query Frame = 0

Query: 7   AAPLPSPPQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAYILAV 66
           AA LP P Q +PSLP RLNSFVA+TR+GKRFKL +RNTTFTTELRAGTATFLTMAYILAV
Sbjct: 4   AADLPLPTQYQPSLPMRLNSFVATTRVGKRFKLTQRNTTFTTELRAGTATFLTMAYILAV 63

Query: 67  NASILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLET 126
           NASILT+SGGTC+ ADCTPLCSDPTVPL+ACTGP HHVIQPD SCKFRPVNPGYAACLET
Sbjct: 64  NASILTESGGTCSAADCTPLCSDPTVPLSACTGPTHHVIQPDISCKFRPVNPGYAACLET 123

Query: 127 TRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALA 186
           TRKDLIIATGASSLIGSF+MGVFANLPLALAPGMGTNAYFA+AVVGFHGSGQLPYRSALA
Sbjct: 124 TRKDLIIATGASSLIGSFIMGVFANLPLALAPGMGTNAYFAFAVVGFHGSGQLPYRSALA 183

Query: 187 AIFIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSAS 246
           AIFIEGLIFLLISAVGLRA+IAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGL+GY+AS
Sbjct: 184 AIFIEGLIFLLISAVGLRAKIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLIGYNAS 243

Query: 247 TLVTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGVVGFVI 306
           TLVT+GACP +SRAALAAVVTAPNGTVSLL NGAVSDQI+CLN RM+SPTFWLGVVGF I
Sbjct: 244 TLVTIGACPKNSRAALAAVVTAPNGTVSLLRNGAVSDQILCLNNRMQSPTFWLGVVGFAI 303

Query: 307 IAYCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTA 366
           IAYCLVK++KG MIYGVVFVTA+SWFRNT VT FP TAAGD+A+ YFKKV+DVH IKTTA
Sbjct: 304 IAYCLVKNIKGAMIYGVVFVTAVSWFRNTVVTVFPDTAAGDSAFNYFKKVIDVHWIKTTA 363

Query: 367 GALSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFMSDAF 426
           GALSFRDLGKPHFWEA+FTFLYVDILDTTGTLYSAARFAGFTDA GDFEGQYFAFMSDAF
Sbjct: 364 GALSFRDLGKPHFWEAMFTFLYVDILDTTGTLYSAARFAGFTDAAGDFEGQYFAFMSDAF 423

Query: 427 SIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGP 486
           SIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGP
Sbjct: 424 SIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGP 483

Query: 487 PLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHLWDWC 546
           PLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIA+GLIGGIGTFVVLHLWDWC
Sbjct: 484 PLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAFGLIGGIGTFVVLHLWDWC 543

Query: 547 GVVLEKLRARKSCCNRNNGRKEIQLSNGNSSASSRERPKTLQV 590
            VVLEKLRAR+ C N NNG KEI LS GNS+AS RERPKTL+V
Sbjct: 544 SVVLEKLRARQICSNGNNGGKEIPLSCGNSNASPRERPKTLEV 586

BLAST of Lcy04g008360.1 vs. ExPASy TrEMBL
Match: A0A6J1EAI3 (adenine/guanine permease AZG1-like OS=Cucurbita moschata OX=3662 GN=LOC111432357 PE=4 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.2e-295
Identity = 526/587 (89.61%), Postives = 554/587 (94.38%), Query Frame = 0

Query: 3   APTTAAPLPSPPQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAY 62
           A   AA LP P Q+KPSLP RLNSFVA+TR+GKRFKL +RNTTFTTELRAGTATFLTMAY
Sbjct: 2   AAAAAAALPPPTQQKPSLPMRLNSFVATTRVGKRFKLTQRNTTFTTELRAGTATFLTMAY 61

Query: 63  ILAVNASILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAA 122
           ILAVNASILTDSGGTC+ ADCTPLCSDPT+PL+ACTGP HHVIQPD SCKFRPVNPGY+A
Sbjct: 62  ILAVNASILTDSGGTCSAADCTPLCSDPTLPLSACTGPTHHVIQPDISCKFRPVNPGYSA 121

Query: 123 CLETTRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYR 182
           CLETTRKDLIIATGASSLIGSF+MGVFANLPLALAPGMGTNAYFA+AVVGFHGSGQLPYR
Sbjct: 122 CLETTRKDLIIATGASSLIGSFIMGVFANLPLALAPGMGTNAYFAFAVVGFHGSGQLPYR 181

Query: 183 SALAAIFIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVG 242
           SALAAIFIEGLIFLLISAVGLRA+IAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGL+G
Sbjct: 182 SALAAIFIEGLIFLLISAVGLRAKIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLIG 241

Query: 243 YSASTLVTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGVV 302
           Y+ASTLVT+GACP +SRAALAAVVTAPNGTVSLL NGAVSDQI+CLN RM+SPTFWLGVV
Sbjct: 242 YNASTLVTIGACPKNSRAALAAVVTAPNGTVSLLRNGAVSDQILCLNNRMQSPTFWLGVV 301

Query: 303 GFVIIAYCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLI 362
           GF IIAYCLVK++KG MI+GVVFVTA+SWFRNT VT FP TAAGD+A+ YFKKV+DVH I
Sbjct: 302 GFAIIAYCLVKNIKGAMIFGVVFVTAVSWFRNTVVTVFPDTAAGDSAFNYFKKVIDVHWI 361

Query: 363 KTTAGALSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFM 422
           KTTAGALSFRDLGKPHFWEA+FTFLYVDILDTTGTLYSAARFAGFTDA GDFEGQYFAFM
Sbjct: 362 KTTAGALSFRDLGKPHFWEAMFTFLYVDILDTTGTLYSAARFAGFTDAAGDFEGQYFAFM 421

Query: 423 SDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPW 482
           SDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPW
Sbjct: 422 SDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPW 481

Query: 483 AVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHL 542
           AVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIA+GLIGGIGTFVVLHL
Sbjct: 482 AVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAFGLIGGIGTFVVLHL 541

Query: 543 WDWCGVVLEKLRARKSCCNRNNGRKEIQLSNGNSSASSRERPKTLQV 590
           WDWCGVVLEKLRAR+ C N NNG KEI LS G    S RERPKTL+V
Sbjct: 542 WDWCGVVLEKLRARQICSNGNNGGKEIPLSGG---ISPRERPKTLEV 585

BLAST of Lcy04g008360.1 vs. ExPASy TrEMBL
Match: A0A6J1GEM4 (adenine/guanine permease AZG1-like OS=Cucurbita moschata OX=3662 GN=LOC111453492 PE=4 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 2.2e-276
Identity = 497/589 (84.38%), Postives = 529/589 (89.81%), Query Frame = 0

Query: 1   MAAPTTAAPLPSPPQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTM 60
           MAAP TAA L      KPSL TRLNSFVA+TR+GKRFKL ERNTTFTTELRAGTATFLTM
Sbjct: 1   MAAPATAAAL----AHKPSLLTRLNSFVATTRVGKRFKLVERNTTFTTELRAGTATFLTM 60

Query: 61  AYILAVNASILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGY 120
           AY+LAVNASILTDSGGTCT ADCTPLCSDPTVPLAAC  PAHHVIQPD SC FRPVNPGY
Sbjct: 61  AYVLAVNASILTDSGGTCTAADCTPLCSDPTVPLAACLAPAHHVIQPDNSCIFRPVNPGY 120

Query: 121 AACLETTRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLP 180
           +ACLETTRKDLI AT  SSLIGSF+MGVFANLPLA+APGMGTNAYFAY VVGFHGSG LP
Sbjct: 121 SACLETTRKDLITATVVSSLIGSFIMGVFANLPLAVAPGMGTNAYFAYTVVGFHGSGYLP 180

Query: 181 YRSALAAIFIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGL 240
           Y+SALAAIFIEGLIFLLISAVGLRA++AKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGL
Sbjct: 181 YKSALAAIFIEGLIFLLISAVGLRAKLAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGL 240

Query: 241 VGYSASTLVTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLG 300
           +G SASTLVT+GACP +SRAALAAVVTAPNGTV+LLPNGAVSD+I+CLN RMESPTFWLG
Sbjct: 241 IGLSASTLVTIGACPKASRAALAAVVTAPNGTVTLLPNGAVSDEILCLNNRMESPTFWLG 300

Query: 301 VVGFVIIAYCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVH 360
           VVGFVIIAYCLVK+ KG M+YG+VFVTAISWFRNTAVT FP TAAGD+AYGYFKKVVD+H
Sbjct: 301 VVGFVIIAYCLVKNAKGAMVYGIVFVTAISWFRNTAVTVFPDTAAGDSAYGYFKKVVDMH 360

Query: 361 LIKTTAGALSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFA 420
           LIKTTAGALSFRDL KPHFWEALFTFLYVDILDTTGTLYS ARFAGFT+A GDFEGQYFA
Sbjct: 361 LIKTTAGALSFRDLRKPHFWEALFTFLYVDILDTTGTLYSIARFAGFTNAAGDFEGQYFA 420

Query: 421 FMSDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIP 480
           FMSDAFSIV+GSLLGTSP+S Y+ESS GIREGGRTGLTAVTVAGYFL++FWFTPLLASIP
Sbjct: 421 FMSDAFSIVIGSLLGTSPLSAYVESSAGIREGGRTGLTAVTVAGYFLLAFWFTPLLASIP 480

Query: 481 PWAVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVL 540
           PWAVGPPLILVGVLMA+SMVEIEWGDMREAIPAF+TM+LMPLTYSIAYGLIGGIG FVVL
Sbjct: 481 PWAVGPPLILVGVLMAKSMVEIEWGDMREAIPAFMTMVLMPLTYSIAYGLIGGIGMFVVL 540

Query: 541 HLWDWCGVVLEKLRARKSCCNRNNGRKEIQLSNGNSSASSRERPKTLQV 590
           HLWDWC VV   LR                  + NSS SS + PKT +V
Sbjct: 541 HLWDWCAVVFNNLR------------------SFNSSPSSTQHPKTPEV 567

BLAST of Lcy04g008360.1 vs. ExPASy TrEMBL
Match: A0A6J1IP75 (adenine/guanine permease AZG1-like OS=Cucurbita maxima OX=3661 GN=LOC111478199 PE=4 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 1.1e-275
Identity = 500/592 (84.46%), Postives = 532/592 (89.86%), Query Frame = 0

Query: 1   MAAPTTA--APLPSP-PQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATF 60
           MAAP TA  A LPSP    K SL TRLNSFVA+T +GKRFKL ERNTTFTTELRAGTATF
Sbjct: 1   MAAPATATEAALPSPITTHKSSLLTRLNSFVATTHVGKRFKLVERNTTFTTELRAGTATF 60

Query: 61  LTMAYILAVNASILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVN 120
           LTMAY+LAVNASILTDSGGTCT ADCTPLCSDPTVPLAAC  PAHHVIQPD SC FRPVN
Sbjct: 61  LTMAYVLAVNASILTDSGGTCTAADCTPLCSDPTVPLAACLAPAHHVIQPDNSCIFRPVN 120

Query: 121 PGYAACLETTRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSG 180
           PGY+ACL TTRKDLI AT ASSLIGSF+MGVFANLPLALAPGMGTNAYFAY VVGFHGSG
Sbjct: 121 PGYSACLVTTRKDLITATVASSLIGSFIMGVFANLPLALAPGMGTNAYFAYTVVGFHGSG 180

Query: 181 QLPYRSALAAIFIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQG 240
            LPY+SAL AIFIEGLIFLLISAVGLRA++AKLIPKPIRISSSAGIGLFLAFIGLQSNQG
Sbjct: 181 YLPYKSALTAIFIEGLIFLLISAVGLRAKLAKLIPKPIRISSSAGIGLFLAFIGLQSNQG 240

Query: 241 IGLVGYSASTLVTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTF 300
           IGL+G+SASTLVT+GACP +SRAAL AVVTAPNGTV+LLPNGAVSD+I+CL+ RMESPTF
Sbjct: 241 IGLIGFSASTLVTIGACPKASRAALVAVVTAPNGTVTLLPNGAVSDEILCLHNRMESPTF 300

Query: 301 WLGVVGFVIIAYCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVV 360
           WLGVVGFVIIAYCLVK+VKG MIYG+VFVTAISWFRNTAVT FP TAAGD+AYGYFKKVV
Sbjct: 301 WLGVVGFVIIAYCLVKNVKGAMIYGIVFVTAISWFRNTAVTVFPDTAAGDSAYGYFKKVV 360

Query: 361 DVHLIKTTAGALSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQ 420
           D+HLIKTTAGALSFRDL KPHFWEALFTFLYVDILDTTGTLYS ARFAGFT+A GDFEGQ
Sbjct: 361 DMHLIKTTAGALSFRDLRKPHFWEALFTFLYVDILDTTGTLYSIARFAGFTNAAGDFEGQ 420

Query: 421 YFAFMSDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLA 480
           YFAFMSDAFSIV+GSLLGTSP+S Y+ESS GIREGGRTGLTAVTVAGYFL+++WFTPLLA
Sbjct: 421 YFAFMSDAFSIVIGSLLGTSPLSAYVESSAGIREGGRTGLTAVTVAGYFLLAYWFTPLLA 480

Query: 481 SIPPWAVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTF 540
           SIPPWAVGPPLILVGVLMA+SMVEIEWGDMREAIPAF+TMILMPLTYSIAYGLIGGIGTF
Sbjct: 481 SIPPWAVGPPLILVGVLMAKSMVEIEWGDMREAIPAFMTMILMPLTYSIAYGLIGGIGTF 540

Query: 541 VVLHLWDWCGVVLEKLRARKSCCNRNNGRKEIQLSNGNSSASSRERPKTLQV 590
           VVLHLWDWC VV  KLR                  + NSSAS  + PKT +V
Sbjct: 541 VVLHLWDWCAVVFNKLR------------------SFNSSASPTQHPKTPEV 574

BLAST of Lcy04g008360.1 vs. ExPASy TrEMBL
Match: A0A5C7GZ26 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_025875 PE=4 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 8.9e-246
Identity = 429/546 (78.57%), Postives = 488/546 (89.38%), Query Frame = 0

Query: 12  SPPQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAYILAVNASIL 71
           SPP+ KPS  TRLNSFVAS+R+GKRFKL ERN+TFTTELRAGTATFLTMAYILAVNASIL
Sbjct: 5   SPPEEKPSHLTRLNSFVASSRVGKRFKLTERNSTFTTELRAGTATFLTMAYILAVNASIL 64

Query: 72  TDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLETTRKDL 131
            DSGGTC+ +DC PLCSD +VP++ CTG  H V+ PD SCKF PVNPGYA+CLE  RKDL
Sbjct: 65  ADSGGTCSVSDCVPLCSDRSVPVSNCTGSTHRVVVPDESCKFNPVNPGYASCLEKIRKDL 124

Query: 132 IIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALAAIFIE 191
           I+AT  SSLIG  +MG FANLPLALAPGMGTNAYFAY VVGFHGSG +PY+SALAA+FIE
Sbjct: 125 IVATVVSSLIGCVIMGAFANLPLALAPGMGTNAYFAYTVVGFHGSGNVPYKSALAAVFIE 184

Query: 192 GLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSASTLVTL 251
           GLIFL ISA+G RA++AKL+PKP+RISSSAGIGLFLAFIGLQ+NQGIGL+GYS+STL+TL
Sbjct: 185 GLIFLFISAIGFRAKLAKLVPKPVRISSSAGIGLFLAFIGLQNNQGIGLIGYSSSTLITL 244

Query: 252 GACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGVVGFVIIAYCL 311
           GACP S+RA+LA V+TA NGT+SL+P   VS  IMCL+GRMESPTFWLGVVGFVIIAYCL
Sbjct: 245 GACPKSARASLAPVITAANGTISLIPGATVSGDIMCLHGRMESPTFWLGVVGFVIIAYCL 304

Query: 312 VKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTAGALSF 371
           VK+VKG MIYG++FVTA+SWFRNT VTAFP T +G++A+ YFKKVVDVHLI++TAGALSF
Sbjct: 305 VKNVKGAMIYGILFVTAVSWFRNTQVTAFPDTDSGNSAHTYFKKVVDVHLIESTAGALSF 364

Query: 372 RDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFMSDAFSIVVG 431
           + +GK +FWEAL TFLYVDILDTTGTLYS ARFAGFTD NGDFEGQYFAFMSDA SIVVG
Sbjct: 365 KSIGKSYFWEALITFLYVDILDTTGTLYSMARFAGFTDQNGDFEGQYFAFMSDATSIVVG 424

Query: 432 SLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGPPLILV 491
           SLLGTSPV+ +IESSTGIREGGRTGLTA+TV GYFL++F+FTPLLASIP WAVGPPLILV
Sbjct: 425 SLLGTSPVTVFIESSTGIREGGRTGLTALTVGGYFLLAFFFTPLLASIPAWAVGPPLILV 484

Query: 492 GVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHLWDWCGVVLE 551
           GVLM +S+VEIEW DMR+AIPAF+T+ILMPLTYSIAYGLIGGIGT++VLH+WDW   +L 
Sbjct: 485 GVLMMKSVVEIEWDDMRQAIPAFVTLILMPLTYSIAYGLIGGIGTYMVLHIWDWFEELLV 544

Query: 552 KLRARK 558
           K    K
Sbjct: 545 KFGVLK 550

BLAST of Lcy04g008360.1 vs. NCBI nr
Match: KAG7027540.1 (Adenine/guanine permease AZG1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1032.3 bits (2668), Expect = 1.6e-297
Identity = 529/587 (90.12%), Postives = 556/587 (94.72%), Query Frame = 0

Query: 3   APTTAAPLPSPPQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAY 62
           A   AA LP P Q+KPSLP RLNSFVA+TR+GKRFKL +RNTTFTTELRAGTATFLTMAY
Sbjct: 2   AAAAAAALPPPTQQKPSLPMRLNSFVATTRVGKRFKLTQRNTTFTTELRAGTATFLTMAY 61

Query: 63  ILAVNASILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAA 122
           ILAVNASILTDSGGTC+ ADCTPLCSDPT+PL+ACTGP HHVIQPD SCKFRPVNPGY+A
Sbjct: 62  ILAVNASILTDSGGTCSAADCTPLCSDPTLPLSACTGPTHHVIQPDISCKFRPVNPGYSA 121

Query: 123 CLETTRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYR 182
           CLETTRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFA+AVVGFHGSGQLPYR
Sbjct: 122 CLETTRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAFAVVGFHGSGQLPYR 181

Query: 183 SALAAIFIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVG 242
           SALAAIFIEGLIFLLISAVGLRA+IAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGL+G
Sbjct: 182 SALAAIFIEGLIFLLISAVGLRAKIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLIG 241

Query: 243 YSASTLVTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGVV 302
           Y+ASTLVT+GACP +SRAALAAVVTAPNGTVSLL NGAVSDQI+CLN RM+SPTFWLGVV
Sbjct: 242 YNASTLVTIGACPKNSRAALAAVVTAPNGTVSLLRNGAVSDQILCLNNRMQSPTFWLGVV 301

Query: 303 GFVIIAYCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLI 362
           GF IIAYCLVK++KG MIYGVVFVTA+SWFRNT VT FP TAAGD+A+ YFKKV+DVH I
Sbjct: 302 GFAIIAYCLVKNIKGAMIYGVVFVTAVSWFRNTVVTVFPDTAAGDSAFNYFKKVIDVHWI 361

Query: 363 KTTAGALSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFM 422
           KTTAGALSFRDLGKPHFWEA+FTFLYVDILDTTGTLYSAARFAGFTDA GDFEGQYFAFM
Sbjct: 362 KTTAGALSFRDLGKPHFWEAMFTFLYVDILDTTGTLYSAARFAGFTDAAGDFEGQYFAFM 421

Query: 423 SDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPW 482
           SDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPW
Sbjct: 422 SDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPW 481

Query: 483 AVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHL 542
           AVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIA+GLIGGIGTFVVLHL
Sbjct: 482 AVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAFGLIGGIGTFVVLHL 541

Query: 543 WDWCGVVLEKLRARKSCCNRNNGRKEIQLSNGNSSASSRERPKTLQV 590
           WDWCGVVLEKLRAR+ C N NNG KEI LS G S+ S RERPKTL+V
Sbjct: 542 WDWCGVVLEKLRARQICSNGNNGGKEIPLSGGISNPSPRERPKTLEV 588

BLAST of Lcy04g008360.1 vs. NCBI nr
Match: KAG6595560.1 (Adenine/guanine permease AZG1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1031.9 bits (2667), Expect = 2.1e-297
Identity = 527/583 (90.39%), Postives = 555/583 (95.20%), Query Frame = 0

Query: 7   AAPLPSPPQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAYILAV 66
           AA LP P Q+KPSLP RLNSFVA+TR+GKRFKL +RNTTFTTELRAGTATFLTMAYILAV
Sbjct: 5   AAALPPPTQQKPSLPMRLNSFVATTRVGKRFKLTQRNTTFTTELRAGTATFLTMAYILAV 64

Query: 67  NASILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLET 126
           NASILTDSGGTC+ ADCTPLCSDPT+PL+ACTGP HHVIQPD SCKFRPVNPGY+ACLET
Sbjct: 65  NASILTDSGGTCSAADCTPLCSDPTLPLSACTGPTHHVIQPDISCKFRPVNPGYSACLET 124

Query: 127 TRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALA 186
           TRKDLIIATGASSLIGSF+MGVFANLPLALAPGMGTNAYFA+AVVGFHGSGQLPYRSALA
Sbjct: 125 TRKDLIIATGASSLIGSFIMGVFANLPLALAPGMGTNAYFAFAVVGFHGSGQLPYRSALA 184

Query: 187 AIFIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSAS 246
           AIFIEGLIFLLISAVGLRA+IAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGL+GY+AS
Sbjct: 185 AIFIEGLIFLLISAVGLRAKIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLIGYNAS 244

Query: 247 TLVTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGVVGFVI 306
           TLVT+GACP +SRAALAAVVTAPNGTVSLL NGAVSDQI+CLN RM+SPTFWLGVVGF I
Sbjct: 245 TLVTIGACPKNSRAALAAVVTAPNGTVSLLRNGAVSDQILCLNNRMQSPTFWLGVVGFAI 304

Query: 307 IAYCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTA 366
           IAYCLVK++KG MIYGVVFVTA+SWFRNT VT FP TAAGD+A+ YFKKV+DVH IKTTA
Sbjct: 305 IAYCLVKNIKGAMIYGVVFVTAVSWFRNTVVTVFPDTAAGDSAFNYFKKVIDVHWIKTTA 364

Query: 367 GALSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFMSDAF 426
           GALSFRDLGKPHFWEA+FTFLYVDILDTTGTLYSAARFAGFTDA GDFEGQYFAFMSDAF
Sbjct: 365 GALSFRDLGKPHFWEAMFTFLYVDILDTTGTLYSAARFAGFTDAAGDFEGQYFAFMSDAF 424

Query: 427 SIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGP 486
           SIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGP
Sbjct: 425 SIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGP 484

Query: 487 PLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHLWDWC 546
           PLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIA+GLIGGIGTFVVLHLWDWC
Sbjct: 485 PLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAFGLIGGIGTFVVLHLWDWC 544

Query: 547 GVVLEKLRARKSCCNRNNGRKEIQLSNGNSSASSRERPKTLQV 590
           GVVLEKLRAR+ C N NNG KEI LS G S+ S RERPKTL+V
Sbjct: 545 GVVLEKLRARQICSNGNNGGKEIPLSGGISNPSPRERPKTLEV 587

BLAST of Lcy04g008360.1 vs. NCBI nr
Match: XP_022966249.1 (adenine/guanine permease AZG1-like [Cucurbita maxima])

HSP 1 Score: 1031.6 bits (2666), Expect = 2.7e-297
Identity = 528/583 (90.57%), Postives = 555/583 (95.20%), Query Frame = 0

Query: 7   AAPLPSPPQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAYILAV 66
           AA LP P Q +PSLP RLNSFVA+TR+GKRFKL +RNTTFTTELRAGTATFLTMAYILAV
Sbjct: 4   AADLPLPTQYQPSLPMRLNSFVATTRVGKRFKLTQRNTTFTTELRAGTATFLTMAYILAV 63

Query: 67  NASILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLET 126
           NASILT+SGGTC+ ADCTPLCSDPTVPL+ACTGP HHVIQPD SCKFRPVNPGYAACLET
Sbjct: 64  NASILTESGGTCSAADCTPLCSDPTVPLSACTGPTHHVIQPDISCKFRPVNPGYAACLET 123

Query: 127 TRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALA 186
           TRKDLIIATGASSLIGSF+MGVFANLPLALAPGMGTNAYFA+AVVGFHGSGQLPYRSALA
Sbjct: 124 TRKDLIIATGASSLIGSFIMGVFANLPLALAPGMGTNAYFAFAVVGFHGSGQLPYRSALA 183

Query: 187 AIFIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSAS 246
           AIFIEGLIFLLISAVGLRA+IAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGL+GY+AS
Sbjct: 184 AIFIEGLIFLLISAVGLRAKIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLIGYNAS 243

Query: 247 TLVTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGVVGFVI 306
           TLVT+GACP +SRAALAAVVTAPNGTVSLL NGAVSDQI+CLN RM+SPTFWLGVVGF I
Sbjct: 244 TLVTIGACPKNSRAALAAVVTAPNGTVSLLRNGAVSDQILCLNNRMQSPTFWLGVVGFAI 303

Query: 307 IAYCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTA 366
           IAYCLVK++KG MIYGVVFVTA+SWFRNT VT FP TAAGD+A+ YFKKV+DVH IKTTA
Sbjct: 304 IAYCLVKNIKGAMIYGVVFVTAVSWFRNTVVTVFPDTAAGDSAFNYFKKVIDVHWIKTTA 363

Query: 367 GALSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFMSDAF 426
           GALSFRDLGKPHFWEA+FTFLYVDILDTTGTLYSAARFAGFTDA GDFEGQYFAFMSDAF
Sbjct: 364 GALSFRDLGKPHFWEAMFTFLYVDILDTTGTLYSAARFAGFTDAAGDFEGQYFAFMSDAF 423

Query: 427 SIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGP 486
           SIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGP
Sbjct: 424 SIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGP 483

Query: 487 PLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHLWDWC 546
           PLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIA+GLIGGIGTFVVLHLWDWC
Sbjct: 484 PLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAFGLIGGIGTFVVLHLWDWC 543

Query: 547 GVVLEKLRARKSCCNRNNGRKEIQLSNGNSSASSRERPKTLQV 590
            VVLEKLRAR+ C N NNG KEI LS GNS+AS RERPKTL+V
Sbjct: 544 SVVLEKLRARQICSNGNNGGKEIPLSCGNSNASPRERPKTLEV 586

BLAST of Lcy04g008360.1 vs. NCBI nr
Match: XP_022924972.1 (adenine/guanine permease AZG1-like [Cucurbita moschata])

HSP 1 Score: 1025.0 bits (2649), Expect = 2.5e-295
Identity = 526/587 (89.61%), Postives = 554/587 (94.38%), Query Frame = 0

Query: 3   APTTAAPLPSPPQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAY 62
           A   AA LP P Q+KPSLP RLNSFVA+TR+GKRFKL +RNTTFTTELRAGTATFLTMAY
Sbjct: 2   AAAAAAALPPPTQQKPSLPMRLNSFVATTRVGKRFKLTQRNTTFTTELRAGTATFLTMAY 61

Query: 63  ILAVNASILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAA 122
           ILAVNASILTDSGGTC+ ADCTPLCSDPT+PL+ACTGP HHVIQPD SCKFRPVNPGY+A
Sbjct: 62  ILAVNASILTDSGGTCSAADCTPLCSDPTLPLSACTGPTHHVIQPDISCKFRPVNPGYSA 121

Query: 123 CLETTRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYR 182
           CLETTRKDLIIATGASSLIGSF+MGVFANLPLALAPGMGTNAYFA+AVVGFHGSGQLPYR
Sbjct: 122 CLETTRKDLIIATGASSLIGSFIMGVFANLPLALAPGMGTNAYFAFAVVGFHGSGQLPYR 181

Query: 183 SALAAIFIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVG 242
           SALAAIFIEGLIFLLISAVGLRA+IAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGL+G
Sbjct: 182 SALAAIFIEGLIFLLISAVGLRAKIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLIG 241

Query: 243 YSASTLVTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGVV 302
           Y+ASTLVT+GACP +SRAALAAVVTAPNGTVSLL NGAVSDQI+CLN RM+SPTFWLGVV
Sbjct: 242 YNASTLVTIGACPKNSRAALAAVVTAPNGTVSLLRNGAVSDQILCLNNRMQSPTFWLGVV 301

Query: 303 GFVIIAYCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLI 362
           GF IIAYCLVK++KG MI+GVVFVTA+SWFRNT VT FP TAAGD+A+ YFKKV+DVH I
Sbjct: 302 GFAIIAYCLVKNIKGAMIFGVVFVTAVSWFRNTVVTVFPDTAAGDSAFNYFKKVIDVHWI 361

Query: 363 KTTAGALSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFM 422
           KTTAGALSFRDLGKPHFWEA+FTFLYVDILDTTGTLYSAARFAGFTDA GDFEGQYFAFM
Sbjct: 362 KTTAGALSFRDLGKPHFWEAMFTFLYVDILDTTGTLYSAARFAGFTDAAGDFEGQYFAFM 421

Query: 423 SDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPW 482
           SDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPW
Sbjct: 422 SDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPW 481

Query: 483 AVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHL 542
           AVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIA+GLIGGIGTFVVLHL
Sbjct: 482 AVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAFGLIGGIGTFVVLHL 541

Query: 543 WDWCGVVLEKLRARKSCCNRNNGRKEIQLSNGNSSASSRERPKTLQV 590
           WDWCGVVLEKLRAR+ C N NNG KEI LS G    S RERPKTL+V
Sbjct: 542 WDWCGVVLEKLRARQICSNGNNGGKEIPLSGG---ISPRERPKTLEV 585

BLAST of Lcy04g008360.1 vs. NCBI nr
Match: XP_038883092.1 (adenine/guanine permease AZG1-like [Benincasa hispida])

HSP 1 Score: 993.0 bits (2566), Expect = 1.1e-285
Identity = 514/592 (86.82%), Postives = 542/592 (91.55%), Query Frame = 0

Query: 1   MAAPTTAAPLPSPPQRKPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTM 60
           MAA T AA  P P Q+ PSLPTRLNSFVA+TR+G+RFKLAERNTTFTTELRAGTATFLTM
Sbjct: 1   MAAETAAALPPPPTQQNPSLPTRLNSFVATTRIGQRFKLAERNTTFTTELRAGTATFLTM 60

Query: 61  AYILAVNASILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGY 120
           AYILAVNASILTDSGGTCT +DCTPLCSD T+PLA CTGP HHV++PD SCKFRPVNPGY
Sbjct: 61  AYILAVNASILTDSGGTCTVSDCTPLCSDSTIPLATCTGPIHHVVKPDNSCKFRPVNPGY 120

Query: 121 AACLETTRKDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLP 180
           AACLETTRKDLIIAT ASSLIGSFVMGVFANLPLALAPGMG NAYFAYAVVGFHGSGQLP
Sbjct: 121 AACLETTRKDLIIATVASSLIGSFVMGVFANLPLALAPGMGINAYFAYAVVGFHGSGQLP 180

Query: 181 YRSALAAIFIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGL 240
           YRSAL AIF+EGLIFLLISAVGLRA+IAKLIPKPIRI+SSAGIGLFLAFIGLQSN+GIGL
Sbjct: 181 YRSALTAIFMEGLIFLLISAVGLRAKIAKLIPKPIRIASSAGIGLFLAFIGLQSNEGIGL 240

Query: 241 VGYSASTLVTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLG 300
           VG+SASTLVT+GACP  SRAALAAVVTAPNGTVSL+PNGAVSDQI+CLN RMESPTFWLG
Sbjct: 241 VGFSASTLVTIGACPKDSRAALAAVVTAPNGTVSLVPNGAVSDQILCLNNRMESPTFWLG 300

Query: 301 VVGFVIIAYCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVH 360
            VGFVIIAYCLVK+VKG MIYGVVFVTA+SWFR T VT FP TA GDAA+GYFKKVVDVH
Sbjct: 301 AVGFVIIAYCLVKNVKGAMIYGVVFVTAVSWFRKTTVTVFPDTAVGDAAFGYFKKVVDVH 360

Query: 361 LIKTTAGALSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFA 420
           LIKTTAGALSF DLGKPHFWEALFTFLYVDILDTTGTLYS ARFAGF DA G+FEGQYFA
Sbjct: 361 LIKTTAGALSFVDLGKPHFWEALFTFLYVDILDTTGTLYSVARFAGFVDAAGNFEGQYFA 420

Query: 421 FMSDAFSIVVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIP 480
           FMSDAF+IVVGSLLGTSPVS YIESSTGIREGGRTGLTAVTVAGYF+M+FWFTPLLASIP
Sbjct: 421 FMSDAFAIVVGSLLGTSPVSAYIESSTGIREGGRTGLTAVTVAGYFMMAFWFTPLLASIP 480

Query: 481 PWAVGPPLILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVL 540
            WAVGPPLILVGVLMA+SMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVL
Sbjct: 481 AWAVGPPLILVGVLMAKSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVL 540

Query: 541 HLWDWCGVVLEKLRARK---SCCNRNNGRKEIQLSNGNSSASSRERPKTLQV 590
           HLWDWC VVL  LR+ K   S  N +N  K  Q  NGNSSASS  RPK ++V
Sbjct: 541 HLWDWCAVVLNNLRSAKSSSSSSNTSNAPKHNQFPNGNSSASSSGRPKIVEV 592

BLAST of Lcy04g008360.1 vs. TAIR 10
Match: AT3G10960.1 (AZA-guanine resistant1 )

HSP 1 Score: 828.2 bits (2138), Expect = 4.3e-240
Identity = 420/545 (77.06%), Postives = 477/545 (87.52%), Query Frame = 0

Query: 10  LPSPPQR-KPSLPTRLNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAYILAVNA 69
           LPS   R KP L  RLN++V S+R+GKRFKLAERN+TFTTELRAGTATFLTMAYILAVNA
Sbjct: 9   LPSTTTRPKPKLLNRLNTYVGSSRVGKRFKLAERNSTFTTELRAGTATFLTMAYILAVNA 68

Query: 70  SILTDSGGTCTTADCTPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLETTR 129
           SIL+DSGGTC+ +DC PLCS+P +  + CTGP   +IQPD SCKF PVNPGYAAC+E  R
Sbjct: 69  SILSDSGGTCSVSDCIPLCSNPAIEPSQCTGPGLRLIQPDVSCKFNPVNPGYAACVEEIR 128

Query: 130 KDLIIATGASSLIGSFVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALAAI 189
           KDLI+AT A+SLIG  +MG+ ANLPLALAPGMGTNAYFAY VVGFHGSG + YR+ALAA+
Sbjct: 129 KDLIVATVAASLIGCVIMGLMANLPLALAPGMGTNAYFAYTVVGFHGSGSISYRTALAAV 188

Query: 190 FIEGLIFLLISAVGLRAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSASTL 249
           FIEGLIFL ISA+G RA++AKL+PKP+RISSSAGIGLFLAFIGLQ+NQGIGLVGYS STL
Sbjct: 189 FIEGLIFLFISAIGFRAKLAKLVPKPVRISSSAGIGLFLAFIGLQNNQGIGLVGYSPSTL 248

Query: 250 VTLGACPLSSRAALAAVVTAPNGTVSLLPNGAVSDQIMCLNGRMESPTFWLGVVGFVIIA 309
           VTL ACP SSR +LA V+T+ NGTVSLL  G+VS  IMC++GRMESPTFWLG+VGFVIIA
Sbjct: 249 VTLAACPASSRISLAPVITSANGTVSLLAGGSVSGDIMCIHGRMESPTFWLGIVGFVIIA 308

Query: 310 YCLVKDVKGGMIYGVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTAGA 369
           YCLVK+VKG MIYG+VFVTA+SWFRNT VTAFP T+AGDAA+ YFKK+VDVH+IK TAGA
Sbjct: 309 YCLVKNVKGAMIYGIVFVTAVSWFRNTEVTAFPNTSAGDAAHDYFKKIVDVHVIKHTAGA 368

Query: 370 LSFRDLGKPHFWEALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFMSDAFSI 429
           LSF  + K HFWEAL TFLYVDILDTTGTLYS ARFAGF D  GDF GQYFAFMSDA +I
Sbjct: 369 LSFSGINKGHFWEALVTFLYVDILDTTGTLYSMARFAGFVDEKGDFAGQYFAFMSDASAI 428

Query: 430 VVGSLLGTSPVSTYIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGPPL 489
           V+GSLLGTSPV+ +IESSTGIREGGRTGLTA+TVA YFL++ +FTPLLASIP WAVGPPL
Sbjct: 429 VIGSLLGTSPVTVFIESSTGIREGGRTGLTAITVAVYFLLAMFFTPLLASIPAWAVGPPL 488

Query: 490 ILVGVLMARSMVEIEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHLWDWCGV 549
           ILVGV+M +S+ EI+W DMREAIPAF+TMILMPLTYS+AYGLIGGIG++VVLHLWDW   
Sbjct: 489 ILVGVMMMKSVTEIDWEDMREAIPAFVTMILMPLTYSVAYGLIGGIGSYVVLHLWDWGEE 548

Query: 550 VLEKL 554
            L KL
Sbjct: 549 GLVKL 553

BLAST of Lcy04g008360.1 vs. TAIR 10
Match: AT5G50300.1 (Xanthine/uracil permease family protein )

HSP 1 Score: 505.8 bits (1301), Expect = 4.8e-143
Identity = 266/523 (50.86%), Postives = 351/523 (67.11%), Query Frame = 0

Query: 24  LNSFVASTRLGKRFKLAERNTTFTTELRAGTATFLTMAYILAVNASILTDSGGTCTTADC 83
           LN  V+ + +G+ FKL  R TTFTTELRA TATFLTMAYI+ VNA+IL DSG TC+  DC
Sbjct: 18  LNDTVSKSFIGRFFKLEARKTTFTTELRAATATFLTMAYIITVNANILADSGATCSINDC 77

Query: 84  TPLCSDPTVPLAACTGPAHHVIQPDGSCKFRPVNPGYAACLETTRKDLIIATGASSLIGS 143
           + + S                  P G       NPGY  C+   +KDL++AT  S+++GS
Sbjct: 78  STVASS----------------SPPGPECVLGSNPGYEQCISRVKKDLVVATSLSAMVGS 137

Query: 144 FVMGVFANLPLALAPGMGTNAYFAYAVVGFHGSGQLPYRSALAAIFIEGLIFLLISAVGL 203
             MG+ ANLP  LAPGMG NAY AY VVGF GSG + Y +A+A + +EG  FL +SA+GL
Sbjct: 138 LAMGLLANLPFGLAPGMGANAYIAYNVVGFRGSGSISYHTAMAIVLLEGCAFLAVSALGL 197

Query: 204 RAQIAKLIPKPIRISSSAGIGLFLAFIGLQSNQGIGLVGYSASTLVTLGACPLSSRAALA 263
           R ++A+LIP+ +R++ + GIG+F+AF+GLQ NQGIGLVG   STLVTL AC         
Sbjct: 198 RGKLARLIPQTVRLACAVGIGMFIAFVGLQMNQGIGLVGPDKSTLVTLTAC--------- 257

Query: 264 AVVTAPNGTVSLLPNGAVSDQI--MCLNGRMESPTFWLGVVGFVIIAYCLVKDVKGGMIY 323
                           A +D +   CL G+M+SPTFWL VVGF+I ++ L+K+VKG MIY
Sbjct: 258 ----------------AETDPVTGACLGGKMKSPTFWLAVVGFLITSFGLMKNVKGSMIY 317

Query: 324 GVVFVTAISWFRNTAVTAFPGTAAGDAAYGYFKKVVDVHLIKTTAGALSFRDLGKPHFWE 383
           G+VFVTAISW R T VT FP T  GD+ Y YF K+VD H I++T GA+SF +  K   W 
Sbjct: 318 GIVFVTAISWIRGTQVTIFPHTPLGDSNYNYFTKIVDFHKIQSTLGAISFTEFRKSEVWV 377

Query: 384 ALFTFLYVDILDTTGTLYSAARFAGFTDANGDFEGQYFAFMSDAFSIVVGSLLGTSPVST 443
           A  T  YVD+L TTG LY+ A   GF + +G FEG+Y A++ DA S VVGS LG +  +T
Sbjct: 378 AFATLFYVDLLGTTGVLYTMAEIGGFVE-DGKFEGEYAAYLVDAGSSVVGSALGVTTTAT 437

Query: 444 YIESSTGIREGGRTGLTAVTVAGYFLMSFWFTPLLASIPPWAVGPPLILVGVLMARSMVE 503
           ++ESS G++EGG+TGLTAV V  YFL S +FTPL+ ++P WAVGP L++VGV+M   + +
Sbjct: 438 FVESSAGLKEGGKTGLTAVIVGLYFLASMFFTPLVTNVPRWAVGPSLVMVGVMMMGVVKD 497

Query: 504 IEWGDMREAIPAFLTMILMPLTYSIAYGLIGGIGTFVVLHLWD 545
           I WG+ +EA+ AF+T++LMPLTYSIA G+I GIG ++ L ++D
Sbjct: 498 IRWGETKEAVTAFVTILLMPLTYSIANGIIAGIGIYLALSMYD 498

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRK76.0e-23977.06Adenine/guanine permease AZG1 OS=Arabidopsis thaliana OX=3702 GN=AZG1 PE=2 SV=1[more]
Q84MA86.8e-14250.86Adenine/guanine permease AZG2 OS=Arabidopsis thaliana OX=3702 GN=AZG2 PE=2 SV=1[more]
L7WRR47.1e-9940.93Efflux pump notK' OS=Aspergillus versicolor OX=46472 GN=notK' PE=1 SV=1[more]
O943007.1e-9941.49Putative xanthine/uracil permease C887.17 OS=Schizosaccharomyces pombe (strain 9... [more]
Q577724.4e-5632.81Putative permease MJ0326 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / D... [more]
Match NameE-valueIdentityDescription
A0A6J1HT631.3e-29790.57adenine/guanine permease AZG1-like OS=Cucurbita maxima OX=3661 GN=LOC111465968 P... [more]
A0A6J1EAI31.2e-29589.61adenine/guanine permease AZG1-like OS=Cucurbita moschata OX=3662 GN=LOC111432357... [more]
A0A6J1GEM42.2e-27684.38adenine/guanine permease AZG1-like OS=Cucurbita moschata OX=3662 GN=LOC111453492... [more]
A0A6J1IP751.1e-27584.46adenine/guanine permease AZG1-like OS=Cucurbita maxima OX=3661 GN=LOC111478199 P... [more]
A0A5C7GZ268.9e-24678.57Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_025875 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KAG7027540.11.6e-29790.12Adenine/guanine permease AZG1, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
KAG6595560.12.1e-29790.39Adenine/guanine permease AZG1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022966249.12.7e-29790.57adenine/guanine permease AZG1-like [Cucurbita maxima][more]
XP_022924972.12.5e-29589.61adenine/guanine permease AZG1-like [Cucurbita moschata][more]
XP_038883092.11.1e-28586.82adenine/guanine permease AZG1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G10960.14.3e-24077.06AZA-guanine resistant1 [more]
AT5G50300.14.8e-14350.86Xanthine/uracil permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006043Nucleobase cation symporter 2 familyPFAMPF00860Xan_ur_permeasecoord: 298..529
e-value: 1.6E-14
score: 53.4
coord: 125..240
e-value: 1.6E-9
score: 36.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 570..589
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR43337:SF12ADENINE/GUANINE PERMEASE AZG1coord: 16..567
IPR045018Azaguanine-like transportersPANTHERPTHR43337XANTHINE/URACIL PERMEASE C887.17-RELATEDcoord: 16..567

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Lcy04g008360Lcy04g008360gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy04g008360.1.exon1Lcy04g008360.1.exon1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy04g008360.1.utr3p1Lcy04g008360.1.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy04g008360.1.cds1Lcy04g008360.1.cds1CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Lcy04g008360.1.utr5p1Lcy04g008360.1.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Lcy04g008360.1Lcy04g008360.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0015851 nucleobase transport
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015205 nucleobase transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity