Tan0008814 (gene) Snake gourd v1

BLAST of Tan0008814 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 5.1e-234
Identity = 429/721 (59.50%), Postives = 518/721 (71.84%), Query Frame = 0

Query: 17  YIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLYSYRNVMSGFSARLTEE 76
           YI+HV  PE  +  +  DLESWY SFLP   T+ +S EQ R++YSY+NV+ GF+A LT+E
Sbjct: 41  YIIHVTGPEGKMLTESEDLESWYHSFLPP--TLMSSEEQPRVIYSYKNVLRGFAASLTQE 100

Query: 77  QVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSKFGEGVIIGVLDTGITP 136
           ++ A+E+K+GFISA P+ +L+  TTHTPK+LGL Q  G+WK S FG+GVIIGVLD+GITP
Sbjct: 101 ELSAVEKKNGFISAHPQRVLHRQTTHTPKFLGLQQDTGVWKESNFGKGVIIGVLDSGITP 160

Query: 137 DHPSFSDDGMPQPPAKWKGGCEFDASI--------------------------------- 196
            HPSFSD G+P PP KWKG C+ + +                                  
Sbjct: 161 GHPSFSDVGIPPPPPKWKGRCDLNVTACNNKLIGARAFNLAAEAMNGKKAEAPIDEDGHG 220

Query: 197 -----TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSSSDILAALDAAIDD 256
                TAAGAFV  A+ LGNA+G AAGMAP AHLAIYKVC  + C  SDILAALDAA++D
Sbjct: 221 THTASTAAGAFVNYAEVLGNAKGTAAGMAPHAHLAIYKVCFGEDCPESDILAALDAAVED 280

Query: 257 GVDVLSLSLG-GPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKSSLSNEAPWILTV 316
           GVDV+S+SLG   P PFF D+ AIG+FAA+QKGIFVSC+AGNSGP  SS+ N APWILTV
Sbjct: 281 GVDVISISLGLSEPPPFFNDSTAIGAFAAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTV 340

Query: 317 GASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR---------------- 376
           GAS  DRRIVA AKLGNG  + GES+FQP  F  T LPL YAG+                
Sbjct: 341 GASTIDRRIVATAKLGNGQEFDGESVFQPSSFTPTLLPLAYAGKNGKEESAFCANGSLDD 400

Query: 377 -----KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLADAHVLPATHVSYT 436
                KVV+CE+GGGI RIAKG  VK AGGAAMIL+N + + FS  AD H LPATHVSY 
Sbjct: 401 SAFRGKVVLCERGGGIARIAKGEEVKRAGGAAMILMNDETNAFSLSADVHALPATHVSYA 460

Query: 437 AGLRIKAYISSSQNPIATISFRGTIIGDTTFSPAMASFSSRGPSRFSSGILKPDITGPGV 496
           AG+ IKAYI+S+  P ATI F+GT+IG+ + +PA+ASFSSRGP+  S GILKPDI GPGV
Sbjct: 461 AGIEIKAYINSTATPTATILFKGTVIGN-SLAPAVASFSSRGPNLPSPGILKPDIIGPGV 520

Query: 497 NILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIHPSWSPAAIKSAIMT 556
           NILAAWPFPL N+T++    TFN+ SGTSMSCPHLSGIAAL+K+ HP WSPAAIKSAIMT
Sbjct: 521 NILAAWPFPLSNSTDSKL--TFNIESGTSMSCPHLSGIAALLKSSHPHWSPAAIKSAIMT 580

Query: 557 SSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGL-YAD 616
           S+D  N   +LIVD+ L+P + FA G+GHVNPSRA DPGLVYDIQPDDYIPYLCGL Y++
Sbjct: 581 SADTINLGNKLIVDETLQPTDLFATGSGHVNPSRANDPGLVYDIQPDDYIPYLCGLGYSE 640

Query: 617 AQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGASQTFKRTVTNVGDAISIYSPIIKAP 676
            +V II HR++ CS    I EG+LNYPSF+V LG+S+TF RTVTNVG+A S Y  I+ AP
Sbjct: 641 TEVGIIAHRKIKCS--ASIPEGELNYPSFSVELGSSKTFTRTVTNVGEAHSSYDLIVAAP 700

BLAST of Tan0008814 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 2.3e-218
Identity = 407/718 (56.69%), Postives = 496/718 (69.08%), Query Frame = 0

Query: 12  TKLQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLYSYRNVMSGFSA 71
           T LQTYIVHV++P+  +  +  DLES+Y SFLP   T++ S   SR+++SY +V +GF+A
Sbjct: 24  TGLQTYIVHVDKPDAQVLANSADLESYYNSFLP--ATVSGSEVPSRIIHSYHHVATGFAA 83

Query: 72  RLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSKFGEGVIIGVLD 131
           +L+ E+VK ME+K GF+SAK E +L LHTTHTP +LGL Q  G W+ S +G+GVIIG+LD
Sbjct: 84  KLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMGFWQESNYGKGVIIGLLD 143

Query: 132 TGITPDHPSFSDDGMPQPPAKWKGGCEFDASI---------------------------- 191
           TGITP HPSFSD  MP PPAKWKG CEF  +                             
Sbjct: 144 TGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARNFISGSGVPPTDEEGHGT 203

Query: 192 ----TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSSSDILAALDAAIDDG 251
               TAAG FV  A   GNA G A GMAPLAH+A+YKVCS  GCS +DILAALDAAIDDG
Sbjct: 204 HTASTAAGNFVNDANVFGNANGTAVGMAPLAHIAMYKVCSEDGCSDADILAALDAAIDDG 263

Query: 252 VDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKSSLSNEAPWILTVGA 311
           VDVLSLSLGG   PF+ DNIAIG+FAAI+KGIFVS SAGN GP  S+LSNEAPWILTVGA
Sbjct: 264 VDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIFVSASAGNDGPLNSTLSNEAPWILTVGA 323

Query: 312 SVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR------------------ 371
           S HDR+IVA A LGNG  Y GES FQP DFP T LPLVY G                   
Sbjct: 324 STHDRKIVATAVLGNGQQYDGESAFQPADFPHTLLPLVYPGTSDEEAAFCSSGSLDKFDV 383

Query: 372 --KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLADAHVLPATHVSYTAGL 431
             KVVVC++GG + R+ K   VK+AGGAAMIL N + DG  T ADAHVLPATHV Y AG 
Sbjct: 384 KGKVVVCDRGGDVARLEKSQTVKDAGGAAMILANLEIDGEGTFADAHVLPATHVGYAAGE 443

Query: 432 RIKAYISSSQNPIATISFRGTIIGDTTFSPAMASFSSRGPSRFSSGILKPDITGPGVNIL 491
            IK+YI+S+  P A I F+GTIIG  + SP+++SFSSRGP+  S GI+KPDI GPGVNIL
Sbjct: 444 MIKSYINSTSTPTAGILFKGTIIGFKS-SPSVSSFSSRGPNLASPGIVKPDIIGPGVNIL 503

Query: 492 AAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIHPSWSPAAIKSAIMTSSD 551
           AAWP  ++N T T+   TFN+ISGTSMSCPHLSGI AL+K+ HP WSPAAIKSAIMTS+D
Sbjct: 504 AAWPVSVENKTGTDL--TFNIISGTSMSCPHLSGIVALLKSAHPDWSPAAIKSAIMTSAD 563

Query: 552 ITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGL-YADAQV 611
            +N  GQ I+D+   PA+ FA GAGHVNPS+A+DPGL+YDIQ +DYI YLCGL Y +  +
Sbjct: 564 QSNLEGQPILDERNLPADIFATGAGHVNPSKASDPGLIYDIQLEDYIQYLCGLGYREKDI 623

Query: 612 SIIVHRRVTCSGVTRIREGDLNYPSFTVTLG-ASQTFKRTVTNVGDAISIYSPIIKAPLG 671
            +IV   V C   + I E +LNYPSF++ LG  +Q + RTVTNVGDA S Y+  I    G
Sbjct: 624 GLIVQETVKCE--SSISEAELNYPSFSIILGPKTQNYTRTVTNVGDASSTYTVNIAKTQG 683

Query: 672 VSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVWISDKHFVRTPISAK 676
           V + V P  L F+K+ ++ TY+V+FT+         F +G + W S+++ VR+PIS K
Sbjct: 684 VDIVVEPATLVFTKMYQQATYTVSFTQSG--DFTDRFVQGAISWSSNEYVVRSPISVK 732

BLAST of Tan0008814 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 2.6e-214
Identity = 402/731 (54.99%), Postives = 497/731 (67.99%), Query Frame = 0

Query: 3   TELSGFSNPTKLQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLYSY 62
           TEL    + + L TYIVHV +P++ I  D  DL ++Y S LP+    +T     R++++Y
Sbjct: 32  TELED-EDQSNLSTYIVHVRKPQV-IQSD--DLHTFYYSLLPE----STKTTNQRIVFTY 91

Query: 63  RNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSKFG 122
           RNV++GF+ +LT E+ KA+++ +  +SA+PE IL+LHTTHTP +LGL Q  GLWK S  G
Sbjct: 92  RNVVNGFAVKLTPEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSG 151

Query: 123 EGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEF----------------------- 182
           +GVIIG+LDTGI+P HPSFSD+GMP PPAKW G CEF                       
Sbjct: 152 KGVIIGILDTGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNFVKTKNLT 211

Query: 183 ----------DASITAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSSSDIL 242
                       + TAAG  V+GA   GNA G A GMAP AH+A+YKVC   GCS S IL
Sbjct: 212 LPFDDVGHGTHTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAIL 271

Query: 243 AALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKSSLSN 302
           A +D A+DDGVDVLSLSLGGP  PFF D IA+G+F AIQKGIFVSCSA NSGP+ SSLSN
Sbjct: 272 AGMDTAVDDGVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSN 331

Query: 303 EAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR-------- 362
           EAPWILTVGAS  DR I+A AKLGNG  Y G+S+FQP DF  + LPLVYAG         
Sbjct: 332 EAPWILTVGASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFSV 391

Query: 363 --------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLADAHV 422
                         KVV+CE GG + R+ KG  VK+AGGAAMIL+N   + F+ +AD HV
Sbjct: 392 FCAPESLNRSDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHV 451

Query: 423 LPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGDTTFSPAMASFSSRGPSRFSSGIL 482
           LPA H+SY AGL +K YI+S+  P ATI F GT+IG+   +P + SFSSRGPS+ S GIL
Sbjct: 452 LPAVHISYEAGLALKEYINSTSTPTATILFEGTVIGN-LLAPQVTSFSSRGPSKASPGIL 511

Query: 483 KPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIHPSWSP 542
           KPDI GPG+NILAAWP  LDN    +T   FN+ISGTSMSCPHLSGIAAL+KN HP WSP
Sbjct: 512 KPDIIGPGLNILAAWPVSLDN----STTPPFNIISGTSMSCPHLSGIAALLKNSHPDWSP 571

Query: 543 AAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIP 602
           AAIKSAIMT++   N  G  I+DQ L PA+ FA GAGHVNP +A DPGLVYDI+P+DYIP
Sbjct: 572 AAIKSAIMTTASQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIP 631

Query: 603 YLCGL-YADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLG-ASQTFKRTVTNVGDAI 662
           YLCGL Y D +V +I+ +RV CS V  I E +LNYPSF++ LG  +Q + RTV NVG A 
Sbjct: 632 YLCGLNYTDREVGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPAN 691

Query: 663 SIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVWISDK 677
           S Y+  I  P+GV ++++P  L F++V +KLTYSV+F      R    F++G L W+S K
Sbjct: 692 STYTAEIGVPVGVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSGK 749

BLAST of Tan0008814 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 4.2e-212
Identity = 410/724 (56.63%), Postives = 496/724 (68.51%), Query Frame = 0

Query: 14  LQTYIVHVEQPEL-GISGDKIDLESWYTSFLPDTITITTSNEQSRLLYSYRNVMSGFSAR 73
           L TYIVHV++ E+ G      +L +W+ SFLP+T      + + R+++SYRNV SGF+ R
Sbjct: 38  LTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPET------SNKDRMVFSYRNVASGFAVR 97

Query: 74  LTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSKFGEGVIIGVLDT 133
           LT E+  A++EK+  +S +PE  L+LHTTHTP +LGL Q  GLW +S  G+GVIIGV+DT
Sbjct: 98  LTPEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDT 157

Query: 134 GITPDHPSFSDDGMPQPPAKWKGGCEF--------------------------------- 193
           GI P H SF+D+GMP PPAKWKG CEF                                 
Sbjct: 158 GIYPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGARNLVKSAIQEPPYEDFFHGT 217

Query: 194 DASITAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPK---GCSSSDILAALDAAI 253
             +  AAG FVEGA   GNARG AAGMAP AHLAIYKVCS K    C  S ILAA+D AI
Sbjct: 218 HTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMDIAI 277

Query: 254 DDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKSSLSNEAPWILT 313
           +DGVDVLSLSLG    PFF D IAIG+FAA QKGIFVSCSA NSGP  SSLSNEAPWILT
Sbjct: 278 EDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPWILT 337

Query: 314 VGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR--------------- 373
           VGAS  DR+I A AKLGNGA Y GE+LFQP DF S  LPLVYA                 
Sbjct: 338 VGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPGSLR 397

Query: 374 ------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLADAHVLPATHVSY 433
                 KVVVC+ GGGI  IAKG  V +AGG+AMIL N +  GF+TLA+AHVLPA HVSY
Sbjct: 398 NINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPAVHVSY 457

Query: 434 TAGLRIKAYISSSQNPIATISFRGTIIGDTTFSPAMASFSSRGPSRFSSGILKPDITGPG 493
            A L IKAYI+S+  P AT+ F+GTIIGD + +P++A+FSSRGPS+ S GILKPDI GPG
Sbjct: 458 AASLAIKAYINSTYTPTATVLFQGTIIGD-SLAPSVAAFSSRGPSQQSPGILKPDIIGPG 517

Query: 494 VNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIHPSWSPAAIKSAIM 553
           VNILAAW   +DN         F++ISGTSMSCPHLSGIAAL+K+ HP WSPAAIKSAIM
Sbjct: 518 VNILAAWAVSVDNKI-----PAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIKSAIM 577

Query: 554 TSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGL-YA 613
           T+++  N RG  I+DQ L+PA+ FA GAGHVNP RA DPGLVYDIQP+DY+PYLCGL Y+
Sbjct: 578 TTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGLGYS 637

Query: 614 DAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGA-SQTFKRTVTNVGDAISIYSPIIK 673
           D +V+IIV R V C  V  I + +LNYPSF++ LG+ SQ + RT+TNVG A S Y+  I 
Sbjct: 638 DREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYTVKID 697

Query: 674 APLGVSVTVTPRNLKFSKVNEKLTYSVTF-TRDDLVRTQSEFSEGYLVWISDKHFVRTPI 677
            PL + ++V+P  + F++VN+K+ Y V F  +    R    F++G + W+SDKH VRTPI
Sbjct: 698 VPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVVRTPI 749

BLAST of Tan0008814 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 6.5e-205
Identity = 393/725 (54.21%), Postives = 479/725 (66.07%), Query Frame = 0

Query: 14  LQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLYSYRNVMSGFSARL 73
           L TYIVHV++ E   S    DL SWY SFLP T        + R+++SYR V SGF+ +L
Sbjct: 35  LMTYIVHVKKSENVASHQSEDLHSWYHSFLPQTFP-----HKERMVFSYRKVASGFAVKL 94

Query: 74  TEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSKFGEGVIIGVLDTG 133
           T E+ K+++EK   +SA+PE  L LHTTHTP +LGL Q  GLW +   G+GVIIG++DTG
Sbjct: 95  TPEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTG 154

Query: 134 ITPDHPSFSDDGMPQPPAKWKGGCEF---------------------------------D 193
           I P HPSF+D+GMP PPAKWKG CEF                                  
Sbjct: 155 IFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGARNLVKSAIQEPPFENFFHGTH 214

Query: 194 ASITAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPK-GCSSSDILAALDAAIDDG 253
            +  AAG F+E A   GNA+G AAGMAP AHLAIYKVC+ K GC+ S ILAA+D AI+DG
Sbjct: 215 TAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAAMDIAIEDG 274

Query: 254 VDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKSSLSNEAPWILTVGA 313
           VDVLSLSLG    PFF D IAIG+FAA Q G+FVSCSA NSGP  S+LSNEAPWILTVGA
Sbjct: 275 VDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEAPWILTVGA 334

Query: 314 SVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR------------------ 373
           S  DR+IVA AKLGNG  Y GE+LFQP DF    LPLVY G                   
Sbjct: 335 STIDRKIVASAKLGNGEEYEGETLFQPKDFSQQLLPLVYPGSFGYGNQTQNQSLCLPGSL 394

Query: 374 -------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLADAHVLPATHVS 433
                  KVV+C+  G +  I KG  V N+GG AMIL N +  GFST A AHVLPA  VS
Sbjct: 395 KNIDLSGKVVLCDV-GNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAHVLPAVEVS 454

Query: 434 YTAGLRIKAYISSSQNPIATISFRGTIIGDTTFSPAMASFSSRGPSRFSSGILKPDITGP 493
           Y AGL IK+YI S+ NP AT+ F+GTIIGD + +P++  FSSRGPS+ S GILKPDI GP
Sbjct: 455 YAAGLTIKSYIKSTYNPTATLIFKGTIIGD-SLAPSVVYFSSRGPSQESPGILKPDIIGP 514

Query: 494 GVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIHPSWSPAAIKSAI 553
           GVNILAAW   +DN         F+++SGTSMSCPHLSGIAALIK+ HP WSPAAIKSAI
Sbjct: 515 GVNILAAWAVSVDNKI-----PAFDIVSGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAI 574

Query: 554 MTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQPDDYIPYLCGL-Y 613
           MT+++  N  G  I+DQ L PA+ FA GAGHVNP +A DPGLVYDI+P+DY+PYLCGL Y
Sbjct: 575 MTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYLCGLGY 634

Query: 614 ADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGA-SQTFKRTVTNVGDAISIYSPII 673
           +D ++ +IV  +V CS V  I E  LNYPSF++ LG+ SQ + RT+TNVG A S Y   +
Sbjct: 635 SDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANSTYKVEL 694

Query: 674 KAPLGVSVTVTPRNLKFSKVNEKLTYSVTF-TRDDLVRTQSEFSEGYLVWISDKHFVRTP 677
           + PL + ++V P  + F++VNEK+++SV F  +    R    F +G L W+SD+H VR P
Sbjct: 695 EVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSDRHAVRIP 747

BLAST of Tan0008814 vs. NCBI nr
Match: XP_038906452.1 (subtilisin-like protease 4 [Benincasa hispida])

HSP 1 Score: 1072.0 bits (2771), Expect = 2.1e-309
Identity = 555/733 (75.72%), Postives = 615/733 (83.90%), Query Frame = 0

Query: 1   MKTELSGFSNPTKLQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLY 60
           MK+ELSG SN  KLQTYIVHV++ EL I GD IDLESWYTSFLP+  TI TSNEQSRLLY
Sbjct: 1   MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPE--TIKTSNEQSRLLY 60

Query: 61  SYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSK 120
           S+RNVMSGFSARLTEE VKAMEEK+GFISA+PE+ILNLHTTHTP+YLGLNQQFGLWK+S 
Sbjct: 61  SFRNVMSGFSARLTEE-VKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSN 120

Query: 121 FGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI----------------- 180
           FG+GVIIGVLDTGI+PDHPSF+DDGMPQPPAKWKG CEF ASI                 
Sbjct: 121 FGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSA 180

Query: 181 ------------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSS 240
                             TAAG FVEGA+ALGNARG+A GMAPLAHLA+YKVCSPKGCSS
Sbjct: 181 LMGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSS 240

Query: 241 SDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKS 300
           SDILAALDAAI DGVDVLSLSLG P  PF+IDNIAIG+FAAI+ GIFVSCSAGNSGPSKS
Sbjct: 241 SDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKS 300

Query: 301 SLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR---- 360
           +L+NEAPWILTVGAS  DR I+ALA+LGNG V+ GESLFQP DFPS+FLPLVYAG     
Sbjct: 301 TLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVK 360

Query: 361 -----------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLAD 420
                            K+VVCE+GGGIGRIAKG+VVKNAGGAAMI+INQ+ D FSTLA+
Sbjct: 361 GSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAE 420

Query: 421 AHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGD--TTFSPAMASFSSRGPSRF 480
           AH+LPATHVSY AGL+IK+YI SSQNP+A+ISF GT+IG   TTFSPAMASFSSRGP   
Sbjct: 421 AHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLP 480

Query: 481 SSGILKPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIH 540
           S GILKPDITGPGVNILAAWPF LDN  NTN  STFNVISGTSMSCPHLSGIAALIK++H
Sbjct: 481 SPGILKPDITGPGVNILAAWPFSLDN--NTNKKSTFNVISGTSMSCPHLSGIAALIKSVH 540

Query: 541 PSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQP 600
           P+WSPAAIKSAIMTS+D+ N +G+ IVDQDLKPANFFAMG+GHVNPS+AADPGLVYDIQP
Sbjct: 541 PNWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQP 600

Query: 601 DDYIPYLCGLYADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGASQTFKRTVTNVG 660
           DDYIPYLCGLY+DAQVSIIV R+VTCS ++RIREGDLNYPSF VTLG SQ F RTVTNVG
Sbjct: 601 DDYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQMFNRTVTNVG 660

Query: 661 DAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVWI 676
           DA SIYS I++AP+GVSV VTP NLKFS+V EK+TYSVTF+RDDLVRT SEFSEGYL+W+
Sbjct: 661 DANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWV 720

BLAST of Tan0008814 vs. NCBI nr
Match: XP_004147599.2 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical protein Csa_000520 [Cucumis sativus])

HSP 1 Score: 1057.7 bits (2734), Expect = 4.1e-305
Identity = 543/734 (73.98%), Postives = 611/734 (83.24%), Query Frame = 0

Query: 1   MKTELSGFSNPTKLQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLY 60
           MKTEL   SN T LQTYIVHV+QPE+ I GD IDL++WYTSFLP+TI   +SNEQSRLLY
Sbjct: 21  MKTELWSASN-TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIE-ASSNEQSRLLY 80

Query: 61  SYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSK 120
           SYR+V+SGFSARLT+EQVK MEEKDGFISA PE+ LNLHTTHTP+YLGLNQ FGLWKNS 
Sbjct: 81  SYRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSN 140

Query: 121 FGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI----------------- 180
           FG+GVIIGVLDTGI P+HPSF+D+GMP PPAKWKG CEF ASI                 
Sbjct: 141 FGKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNV 200

Query: 181 ------------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSS 240
                             TAAG FV+GA+ALGNARG+A GMAPLAH+A+YKVCSPKGCSS
Sbjct: 201 SIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSS 260

Query: 241 SDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKS 300
           SDILAALDAAIDDGVDVLSLSLG P +PFF D IA+G+FAAI+KGIFVSCSAGNSGPSK+
Sbjct: 261 SDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKN 320

Query: 301 SLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR---- 360
           +L+NEAPWILTVGAS  DR+IVALAKL +G V++GESLFQP DF S FLPLVYAG+    
Sbjct: 321 TLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIE 380

Query: 361 -----------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLAD 420
                            K+VVCE+GGGIGRIAKGLVVKN GGAAMIL+NQ+PDGFSTLA+
Sbjct: 381 GSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAE 440

Query: 421 AHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGD--TTFSPAMASFSSRGPSRF 480
           AHVLP TH+SY  GL+IK YI+SS NP A+ISF GT++G+  TTFSPAMASFSSRGP + 
Sbjct: 441 AHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQA 500

Query: 481 SSGILKPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIH 540
           S GILKPDITGPGVNILAAWPFPL+NNTNTNT STFNVISGTSMSCPHLSGIAALIK+ H
Sbjct: 501 SPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNH 560

Query: 541 PSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQP 600
           P+WSPAAIKSAIMTS+D+ NP+G+ IVDQDLKPANFFAMG+GHVNPS+AA+PGLVYDIQP
Sbjct: 561 PNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQP 620

Query: 601 DDYIPYLCGLYADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGA-SQTFKRTVTNV 660
           DDY+PYLC LY DAQVSIIV R+VTCS V+RIREGDLNYPSF V+LGA SQ F RTVTNV
Sbjct: 621 DDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNV 680

Query: 661 GDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVW 676
           GDA S+Y  I+KAP GVSV VTPRNLKFSK+NEKLTYSVTF+R D VRT+SEFSEGYL+W
Sbjct: 681 GDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIW 740

BLAST of Tan0008814 vs. NCBI nr
Match: XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 1047.3 bits (2707), Expect = 5.5e-302
Identity = 540/734 (73.57%), Postives = 609/734 (82.97%), Query Frame = 0

Query: 1   MKTELSGFSNPTKLQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLY 60
           MKTELS  SN T LQTYIVHV+QP L I GD IDL++WYTSFLP+TI   +S+EQ RLLY
Sbjct: 21  MKTELSSTSN-TNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIE-ASSDEQPRLLY 80

Query: 61  SYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSK 120
           SYR+VMSGFSARLT+EQVKAMEEKDGFISA PE+ LNLHTTHTP+YLGLN+ FGLWKNS 
Sbjct: 81  SYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNKHFGLWKNSN 140

Query: 121 FGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI----------------- 180
           FG+GVIIGVLDTGI P+HPSF+D+GM  PPAKWKG CEF ASI                 
Sbjct: 141 FGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNF 200

Query: 181 ------------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSS 240
                             TAAG FV+GA+ALGNARG+A GMAPLAH+A+YKVCSP GCSS
Sbjct: 201 VIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSS 260

Query: 241 SDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKS 300
           SDILAALDAAIDDGVDVLSLSLG P +PFF D IA+G+FAAI+KGIFVSCSAGNSGPSK+
Sbjct: 261 SDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAIKKGIFVSCSAGNSGPSKN 320

Query: 301 SLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR---- 360
           +L+NEAPWILTVGAS  DR+IVALAKLG+G VY+GESLFQP +FPS FLPLVYAG+    
Sbjct: 321 TLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIE 380

Query: 361 -----------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLAD 420
                            KVVVCE+GGGI R+AKGLVVKN GGAAMILINQ+P+GFSTLA+
Sbjct: 381 GSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAE 440

Query: 421 AHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGD--TTFSPAMASFSSRGPSRF 480
           AHVLP TH+SY  GL+IKAYI+SS NP A+ISF+GT++G+  TTFSPAMASFSSRGP + 
Sbjct: 441 AHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQA 500

Query: 481 SSGILKPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIH 540
           S GILKPDITGPGVNILAAWPFPL+NNTNTNT STFNVISGTSMSCPHLSGIAALIK+ H
Sbjct: 501 SPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYH 560

Query: 541 PSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQP 600
           P+WSPAAIKSAIMTS+D+ NP+G+ I+DQDLKPANFFAMG+GHVNPS+AA+PGLVYDIQP
Sbjct: 561 PNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQP 620

Query: 601 DDYIPYLCGLYADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGA-SQTFKRTVTNV 660
           DDYIPYLC LY DAQVSIIV ++VTCS V RIREGDLNYPSF V+LGA SQTF RTVTNV
Sbjct: 621 DDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNV 680

Query: 661 GDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVW 676
           GDA S+Y PI++AP GVSV VTP NLKFSK+NEK+TYSVTF+R D VRT SEFSEGYL+W
Sbjct: 681 GDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIW 740

BLAST of Tan0008814 vs. NCBI nr
Match: KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1044.6 bits (2700), Expect = 3.6e-301
Identity = 540/734 (73.57%), Postives = 607/734 (82.70%), Query Frame = 0

Query: 1   MKTELSGFSNPTKLQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLY 60
           MKTELS  SN T LQTYIVHV+QP L I GD IDL++WYTSFLP+TI   +S+EQ RLLY
Sbjct: 1   MKTELSSTSN-TNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIE-ASSDEQPRLLY 60

Query: 61  SYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSK 120
           SYR+VMSGFSARLT+EQVKAMEEKDGFISA PE+ LNLHTTHTP+YLGLN  FGLWKNS 
Sbjct: 61  SYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSN 120

Query: 121 FGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI----------------- 180
           FG+GVIIGVLDTGI P+HPSF+D+GM  PPAKWKG CEF ASI                 
Sbjct: 121 FGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNF 180

Query: 181 ------------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSS 240
                             TAAG FV+GA+ALGNARG+A GMAPLAH+A+YKVCSP GCSS
Sbjct: 181 VIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSS 240

Query: 241 SDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKS 300
           SDILAALDAAIDDGVDVLSLSLG P +PFF D IAIG+FAAI+KGIFVSCSAGNSGPSK+
Sbjct: 241 SDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKN 300

Query: 301 SLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR---- 360
           +L+NEAPWILTVGAS  DR+IVALAKLG+G VY+GESLFQP +FPS FLPLVYAG+    
Sbjct: 301 TLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIE 360

Query: 361 -----------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLAD 420
                            KVVVCE+GGGI R+AKGLVVKN GGAAMILINQ+P+GFSTLA+
Sbjct: 361 GSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAE 420

Query: 421 AHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGD--TTFSPAMASFSSRGPSRF 480
           AHVLP TH+SY  GL+IKAYI+SS NP A+ISF+GT++G+  TTFSPAMASFSSRGP + 
Sbjct: 421 AHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQA 480

Query: 481 SSGILKPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIH 540
           S GILKPDITGPGVNILAAWPFPL+NNTNTNT STFNVISGTSMSCPHLSGIAALIK+ H
Sbjct: 481 SPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYH 540

Query: 541 PSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQP 600
           P+WSPAAIKSAIMTS+D+ NP+G+ I+DQDLKPANFFAMG+GHVNPS+AA+PGLVYDIQP
Sbjct: 541 PNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQP 600

Query: 601 DDYIPYLCGLYADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGA-SQTFKRTVTNV 660
           DDYIPYLC LY DAQVSIIV ++VTCS V RIREGDLNYPSF V+L A SQTF RTVTNV
Sbjct: 601 DDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNV 660

Query: 661 GDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVW 676
           GDA S+Y PI++AP GVSV VTP NLKFSK+NEK+TYSVTF+R D VRT SEFSEGYL+W
Sbjct: 661 GDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIW 720

BLAST of Tan0008814 vs. NCBI nr
Match: KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 951.4 bits (2458), Expect = 4.1e-273
Identity = 498/737 (67.57%), Postives = 577/737 (78.29%), Query Frame = 0

Query: 2   KTELSGFSNPTKLQTYIVHVEQPELGISGDKID--LESWYTSFLPDTITITTSNEQSRLL 61
           K EL      +KLQTYIVHVEQP++ + G+  +  +ESWY SF+P +   T   EQ +LL
Sbjct: 22  KVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTV--EQPQLL 81

Query: 62  YSYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNS 121
           YSYRNVMSGFSARLT EQVKAME+KDGFISA PE+I++LHTTHTP+YLGLNQQFG+WKNS
Sbjct: 82  YSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNS 141

Query: 122 KFGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI---------------- 181
            FG+GVI+GVLDTGI P+HPSF+D+GM  PPAKWKG CEF++S+                
Sbjct: 142 NFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNN 201

Query: 182 -------------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCS 241
                              TAAGAFVE A+ALGNA+G+AAG+APLAHLAIYKVCS K C 
Sbjct: 202 FLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCP 261

Query: 242 SSDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSK 301
           SSD+ A +DAAIDDGVDVLS+SLG    PFF DNIA+ +F AIQKGIFVS SAGNSGP  
Sbjct: 262 SSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLN 321

Query: 302 SSLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR--- 361
           S+LSN+APWILTVGAS  +RRIVA+AKLGNG  Y GESL+QP DFPS FLPLVYAG    
Sbjct: 322 STLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNREN 381

Query: 362 ------------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLA 421
                             KVVVCE  GG+GR+AKGLVVKNAGGAAMILINQ+ DGFSTL+
Sbjct: 382 KTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLS 441

Query: 422 DAHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGD-TTFS-PAMASFSSRGPSR 481
           +AHVLPATHVSY AG+ IK+YI+SSQNP A+ISF+GT+IGD   FS P+MASFSSRGP  
Sbjct: 442 EAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCL 501

Query: 482 FSSGILKPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNI 541
            S GILKPDITGPGVNILAAWPFPLDN  +TNT STFNVISGTSMSCPHLSGIAALIK++
Sbjct: 502 PSPGILKPDITGPGVNILAAWPFPLDN--DTNTKSTFNVISGTSMSCPHLSGIAALIKSV 561

Query: 542 HPSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQ 601
           HP+WSPAAIKSAIMT+++I  P+G+ IVDQDL+PANFFAMGAGHVNPS+AADPGLVYDIQ
Sbjct: 562 HPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQ 621

Query: 602 PDDYIPYLCGLYADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGASQTFKRTVTNV 661
           PDDYIPYLCGLY D +VSIIVHR V C  V  IREGDLNYPSF+V LG  QTFKRTVTNV
Sbjct: 622 PDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNYPSFSVALGGLQTFKRTVTNV 681

Query: 662 GDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVW 677
           G+A S+Y+ I++APLGVS+TV PR L FS+VN+ +T++VTF R   V+   EF EGYL W
Sbjct: 682 GEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKW 741

BLAST of Tan0008814 vs. ExPASy TrEMBL
Match: A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)

HSP 1 Score: 1057.7 bits (2734), Expect = 2.0e-305
Identity = 543/734 (73.98%), Postives = 611/734 (83.24%), Query Frame = 0

Query: 1   MKTELSGFSNPTKLQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLY 60
           MKTEL   SN T LQTYIVHV+QPE+ I GD IDL++WYTSFLP+TI   +SNEQSRLLY
Sbjct: 1   MKTELWSASN-TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIE-ASSNEQSRLLY 60

Query: 61  SYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSK 120
           SYR+V+SGFSARLT+EQVK MEEKDGFISA PE+ LNLHTTHTP+YLGLNQ FGLWKNS 
Sbjct: 61  SYRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSN 120

Query: 121 FGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI----------------- 180
           FG+GVIIGVLDTGI P+HPSF+D+GMP PPAKWKG CEF ASI                 
Sbjct: 121 FGKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNV 180

Query: 181 ------------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSS 240
                             TAAG FV+GA+ALGNARG+A GMAPLAH+A+YKVCSPKGCSS
Sbjct: 181 SIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSS 240

Query: 241 SDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKS 300
           SDILAALDAAIDDGVDVLSLSLG P +PFF D IA+G+FAAI+KGIFVSCSAGNSGPSK+
Sbjct: 241 SDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKN 300

Query: 301 SLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR---- 360
           +L+NEAPWILTVGAS  DR+IVALAKL +G V++GESLFQP DF S FLPLVYAG+    
Sbjct: 301 TLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIE 360

Query: 361 -----------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLAD 420
                            K+VVCE+GGGIGRIAKGLVVKN GGAAMIL+NQ+PDGFSTLA+
Sbjct: 361 GSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAE 420

Query: 421 AHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGD--TTFSPAMASFSSRGPSRF 480
           AHVLP TH+SY  GL+IK YI+SS NP A+ISF GT++G+  TTFSPAMASFSSRGP + 
Sbjct: 421 AHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQA 480

Query: 481 SSGILKPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIH 540
           S GILKPDITGPGVNILAAWPFPL+NNTNTNT STFNVISGTSMSCPHLSGIAALIK+ H
Sbjct: 481 SPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNH 540

Query: 541 PSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQP 600
           P+WSPAAIKSAIMTS+D+ NP+G+ IVDQDLKPANFFAMG+GHVNPS+AA+PGLVYDIQP
Sbjct: 541 PNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQP 600

Query: 601 DDYIPYLCGLYADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGA-SQTFKRTVTNV 660
           DDY+PYLC LY DAQVSIIV R+VTCS V+RIREGDLNYPSF V+LGA SQ F RTVTNV
Sbjct: 601 DDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNV 660

Query: 661 GDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVW 676
           GDA S+Y  I+KAP GVSV VTPRNLKFSK+NEKLTYSVTF+R D VRT+SEFSEGYL+W
Sbjct: 661 GDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIW 720

BLAST of Tan0008814 vs. ExPASy TrEMBL
Match: A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 2.7e-302
Identity = 540/734 (73.57%), Postives = 609/734 (82.97%), Query Frame = 0

Query: 1   MKTELSGFSNPTKLQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLY 60
           MKTELS  SN T LQTYIVHV+QP L I GD IDL++WYTSFLP+TI   +S+EQ RLLY
Sbjct: 21  MKTELSSTSN-TNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIE-ASSDEQPRLLY 80

Query: 61  SYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSK 120
           SYR+VMSGFSARLT+EQVKAMEEKDGFISA PE+ LNLHTTHTP+YLGLN+ FGLWKNS 
Sbjct: 81  SYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNKHFGLWKNSN 140

Query: 121 FGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI----------------- 180
           FG+GVIIGVLDTGI P+HPSF+D+GM  PPAKWKG CEF ASI                 
Sbjct: 141 FGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNF 200

Query: 181 ------------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSS 240
                             TAAG FV+GA+ALGNARG+A GMAPLAH+A+YKVCSP GCSS
Sbjct: 201 VIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSS 260

Query: 241 SDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKS 300
           SDILAALDAAIDDGVDVLSLSLG P +PFF D IA+G+FAAI+KGIFVSCSAGNSGPSK+
Sbjct: 261 SDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAIKKGIFVSCSAGNSGPSKN 320

Query: 301 SLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR---- 360
           +L+NEAPWILTVGAS  DR+IVALAKLG+G VY+GESLFQP +FPS FLPLVYAG+    
Sbjct: 321 TLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIE 380

Query: 361 -----------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLAD 420
                            KVVVCE+GGGI R+AKGLVVKN GGAAMILINQ+P+GFSTLA+
Sbjct: 381 GSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAE 440

Query: 421 AHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGD--TTFSPAMASFSSRGPSRF 480
           AHVLP TH+SY  GL+IKAYI+SS NP A+ISF+GT++G+  TTFSPAMASFSSRGP + 
Sbjct: 441 AHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQA 500

Query: 481 SSGILKPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIH 540
           S GILKPDITGPGVNILAAWPFPL+NNTNTNT STFNVISGTSMSCPHLSGIAALIK+ H
Sbjct: 501 SPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYH 560

Query: 541 PSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQP 600
           P+WSPAAIKSAIMTS+D+ NP+G+ I+DQDLKPANFFAMG+GHVNPS+AA+PGLVYDIQP
Sbjct: 561 PNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQP 620

Query: 601 DDYIPYLCGLYADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGA-SQTFKRTVTNV 660
           DDYIPYLC LY DAQVSIIV ++VTCS V RIREGDLNYPSF V+LGA SQTF RTVTNV
Sbjct: 621 DDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNV 680

Query: 661 GDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVW 676
           GDA S+Y PI++AP GVSV VTP NLKFSK+NEK+TYSVTF+R D VRT SEFSEGYL+W
Sbjct: 681 GDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIW 740

BLAST of Tan0008814 vs. ExPASy TrEMBL
Match: A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)

HSP 1 Score: 1044.6 bits (2700), Expect = 1.7e-301
Identity = 540/734 (73.57%), Postives = 607/734 (82.70%), Query Frame = 0

Query: 1   MKTELSGFSNPTKLQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLY 60
           MKTELS  SN T LQTYIVHV+QP L I GD IDL++WYTSFLP+TI   +S+EQ RLLY
Sbjct: 1   MKTELSSTSN-TNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIE-ASSDEQPRLLY 60

Query: 61  SYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSK 120
           SYR+VMSGFSARLT+EQVKAMEEKDGFISA PE+ LNLHTTHTP+YLGLN  FGLWKNS 
Sbjct: 61  SYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSN 120

Query: 121 FGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI----------------- 180
           FG+GVIIGVLDTGI P+HPSF+D+GM  PPAKWKG CEF ASI                 
Sbjct: 121 FGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNF 180

Query: 181 ------------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSS 240
                             TAAG FV+GA+ALGNARG+A GMAPLAH+A+YKVCSP GCSS
Sbjct: 181 VIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSS 240

Query: 241 SDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKS 300
           SDILAALDAAIDDGVDVLSLSLG P +PFF D IAIG+FAAI+KGIFVSCSAGNSGPSK+
Sbjct: 241 SDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKN 300

Query: 301 SLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR---- 360
           +L+NEAPWILTVGAS  DR+IVALAKLG+G VY+GESLFQP +FPS FLPLVYAG+    
Sbjct: 301 TLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIE 360

Query: 361 -----------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLAD 420
                            KVVVCE+GGGI R+AKGLVVKN GGAAMILINQ+P+GFSTLA+
Sbjct: 361 GSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAE 420

Query: 421 AHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGD--TTFSPAMASFSSRGPSRF 480
           AHVLP TH+SY  GL+IKAYI+SS NP A+ISF+GT++G+  TTFSPAMASFSSRGP + 
Sbjct: 421 AHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQA 480

Query: 481 SSGILKPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNIH 540
           S GILKPDITGPGVNILAAWPFPL+NNTNTNT STFNVISGTSMSCPHLSGIAALIK+ H
Sbjct: 481 SPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYH 540

Query: 541 PSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQP 600
           P+WSPAAIKSAIMTS+D+ NP+G+ I+DQDLKPANFFAMG+GHVNPS+AA+PGLVYDIQP
Sbjct: 541 PNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQP 600

Query: 601 DDYIPYLCGLYADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGA-SQTFKRTVTNV 660
           DDYIPYLC LY DAQVSIIV ++VTCS V RIREGDLNYPSF V+L A SQTF RTVTNV
Sbjct: 601 DDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNV 660

Query: 661 GDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVW 676
           GDA S+Y PI++AP GVSV VTP NLKFSK+NEK+TYSVTF+R D VRT SEFSEGYL+W
Sbjct: 661 GDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIW 720

BLAST of Tan0008814 vs. ExPASy TrEMBL
Match: A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 2.0e-273
Identity = 498/737 (67.57%), Postives = 577/737 (78.29%), Query Frame = 0

Query: 2   KTELSGFSNPTKLQTYIVHVEQPELGISGDKID--LESWYTSFLPDTITITTSNEQSRLL 61
           K EL      +KLQTYIVHVEQP++ + G+  +  +ESWY SF+P +   T   EQ +LL
Sbjct: 22  KVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTV--EQPQLL 81

Query: 62  YSYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNS 121
           YSYRNVMSGFSARLT EQVKAME+KDGFISA PE+I++LHTTHTP+YLGLNQQFG+WKNS
Sbjct: 82  YSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNS 141

Query: 122 KFGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI---------------- 181
            FG+GVI+GVLDTGI P+HPSF+D+GM  PPAKWKG CEF++S+                
Sbjct: 142 NFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNN 201

Query: 182 -------------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCS 241
                              TAAGAFVE A+ALGNA+G+AAG+APLAHLAIYKVCS K C 
Sbjct: 202 FLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCP 261

Query: 242 SSDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSK 301
           SSD+ A +DAAIDDGVDVLS+SLG    PFF DNIA+ +F AIQKGIFVS SAGNSGP  
Sbjct: 262 SSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLN 321

Query: 302 SSLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR--- 361
           S+LSN+APWILTVGAS  +RRIVA+AKLGNG  Y GESL+QP DFPS FLPLVYAG    
Sbjct: 322 STLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNREN 381

Query: 362 ------------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLA 421
                             KVVVCE  GG+GR+AKGLVVKNAGGAAMILINQ+ DGFSTL+
Sbjct: 382 KTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLS 441

Query: 422 DAHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGD-TTFS-PAMASFSSRGPSR 481
           +AHVLPATHVSY AG+ IK+YI+SSQNP A+ISF+GT+IGD   FS P+MASFSSRGP  
Sbjct: 442 EAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCL 501

Query: 482 FSSGILKPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNI 541
            S GILKPDITGPGVNILAAWPFPLDN  +TNT STFNVISGTSMSCPHLSGIAALIK++
Sbjct: 502 PSPGILKPDITGPGVNILAAWPFPLDN--DTNTKSTFNVISGTSMSCPHLSGIAALIKSV 561

Query: 542 HPSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQ 601
           HP+WSPAAIKSAIMT+++I  P+G+ IVDQDL+PANFFAMGAGHVNPS+AADPGLVYDIQ
Sbjct: 562 HPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQ 621

Query: 602 PDDYIPYLCGLYADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGASQTFKRTVTNV 661
           PDDYIPYLCGLY D +VSIIVHR V C  V  IREGDLNYPSF+V LG  QTFKRTVTNV
Sbjct: 622 PDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNYPSFSVALGGLQTFKRTVTNV 681

Query: 662 GDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVW 677
           G+A S+Y+ I++APLGVS+TV PR L FS+VN+ +T++VTF R   V+   EF EGYL W
Sbjct: 682 GEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKW 741

BLAST of Tan0008814 vs. ExPASy TrEMBL
Match: A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 5.8e-273
Identity = 497/737 (67.44%), Postives = 576/737 (78.15%), Query Frame = 0

Query: 2   KTELSGFSNPTKLQTYIVHVEQPELGISGDKID--LESWYTSFLPDTITITTSNEQSRLL 61
           K EL      +KLQTYIVHVEQP++ + G+  +  +ESWY SF+P +   T   EQ +LL
Sbjct: 31  KVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTV--EQPQLL 90

Query: 62  YSYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNS 121
           YSYRNVMSGFSARLT EQVKAME+KDGFISA PE+I++LHTTHTP+YLGLNQQFG+WKNS
Sbjct: 91  YSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNS 150

Query: 122 KFGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI---------------- 181
            FG+GVI+GVLDTGI P+HPSF+D+GM  PPAKWKG CEF++S+                
Sbjct: 151 NFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNN 210

Query: 182 -------------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCS 241
                              TAAGAFVE A+ALGNA+G+AAG+APLAHLAIYKVCS K C 
Sbjct: 211 FLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCP 270

Query: 242 SSDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSK 301
           SSD+ A +DAAIDDGVDVLS+SLG    PFF DNIA+ +F AIQKGIFVS SAGNSGP  
Sbjct: 271 SSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLN 330

Query: 302 SSLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR--- 361
           S+LSN+APWILTVGAS  +RRIVA+AKLGNG  Y GESL+QP DFPS FLPLVYAG    
Sbjct: 331 STLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNREN 390

Query: 362 ------------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLA 421
                             KVVVCE  GG+GR+AKGLVVKNAGGAAMILINQ+ DGFSTL+
Sbjct: 391 KTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLS 450

Query: 422 DAHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGD-TTFS-PAMASFSSRGPSR 481
           +AHVLPATHVSY AG+ IK+YI+SSQNP A+ISF+GT+IGD   FS P+MASFSSRGP  
Sbjct: 451 EAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCL 510

Query: 482 FSSGILKPDITGPGVNILAAWPFPLDNNTNTNTNSTFNVISGTSMSCPHLSGIAALIKNI 541
            S GILKPDITGPGVNILAAWPFPLDN  +TNT STFNVISGTSMSCPHLSGIAALIK++
Sbjct: 511 PSPGILKPDITGPGVNILAAWPFPLDN--DTNTKSTFNVISGTSMSCPHLSGIAALIKSV 570

Query: 542 HPSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAGHVNPSRAADPGLVYDIQ 601
           HP+WSPAAIKSAIMT+++I  P+G+ I DQDL+PANFFAMGAGHVNPS+AADPGLVYDIQ
Sbjct: 571 HPNWSPAAIKSAIMTTANIKTPQGEPITDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQ 630

Query: 602 PDDYIPYLCGLYADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGASQTFKRTVTNV 661
           PDDYIPYLCGLY D +VSIIVHR V C  V  IREGDLNYPSF+V LG  QTFKRTVTNV
Sbjct: 631 PDDYIPYLCGLYKDEEVSIIVHRTVICGLVLSIREGDLNYPSFSVALGGLQTFKRTVTNV 690

Query: 662 GDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVRTQSEFSEGYLVW 677
           G+A S+Y+ I++APLGVS+TV PR L FS+VN+ +T++VTF R   V+   EF EGYL W
Sbjct: 691 GEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGEFGEGYLKW 750

BLAST of Tan0008814 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 523.5 bits (1347), Expect = 2.6e-148
Identity = 320/759 (42.16%), Postives = 430/759 (56.65%), Query Frame = 0

Query: 6   SGFSNPTKLQTYIV--HVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQ---SRLLY 65
           S  S   + QTYIV  H           K D   W+ SFL + +      E+   SRLLY
Sbjct: 17  SSSSEILQKQTYIVQLHPNSETAKTFASKFD---WHLSFLQEAVLGVEEEEEEPSSRLLY 76

Query: 66  SYRNVMSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLN--QQFGLWKN 125
           SY + + GF+A+LTE + + +      ++ +P+ +L + TT++ K+LGL+     G+W  
Sbjct: 77  SYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSK 136

Query: 126 SKFGEGVIIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCEFDASI--------------- 185
           S+FG+G IIGVLDTG+ P+ PSF D GMP  P KWKG C+   S                
Sbjct: 137 SRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFF 196

Query: 186 -----------------------------------TAAGAFVEGAQALGNARGEAAGMAP 245
                                              T  G+ V  A  LGN  G A GMAP
Sbjct: 197 IRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAP 256

Query: 246 LAHLAIYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQ 305
            AH+A+YKVC   GC SSDILAA+D AI D VDVLSLSLGG P P + D IAIG+F A++
Sbjct: 257 GAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAME 316

Query: 306 KGIFVSCSAGNSGPSKSSLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPM- 365
           +GI V C+AGN+GP +SS++N APW+ T+GA   DRR  A+ +L NG +  GESL+    
Sbjct: 317 RGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKG 376

Query: 366 ----------------DFPSTF-----LPLVYAGRKVVVCEKGGGIGRIAKGLVVKNAGG 425
                           D  S F     LP      K+V+C++G   GR  KG  VK AGG
Sbjct: 377 IKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGG 436

Query: 426 AAMILINQQPDGFSTLADAHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGDTT 485
            AMIL N + +      D H+LPAT + YT  + +KAY++++  P A I F GT+IG + 
Sbjct: 437 VAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGRSR 496

Query: 486 FSPAMASFSSRGPSRFSSGILKPDITGPGVNILAAWPFPLDNN--TNTNTNSTFNVISGT 545
            +P +A FS+RGPS  +  ILKPD+  PGVNI+AAWP  L        +    F V+SGT
Sbjct: 497 -APEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFTVMSGT 556

Query: 546 SMSCPHLSGIAALIKNIHPSWSPAAIKSAIMTSSDITNPRGQLIVDQDLKPANFFAMGAG 605
           SMSCPH+SGI ALI++ +P+WSPAAIKSA+MT++D+ + +G+ I D + KPA  FA+GAG
Sbjct: 557 SMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAG 616

Query: 606 HVNPSRAADPGLVYDIQPDDYIPYLCGL-YADAQVSIIVHRRVTCSGVTRIREG-DLNYP 665
           HVNP +A +PGLVY+IQP DYI YLC L +  + +  I H+ V+C+G+ R   G  LNYP
Sbjct: 617 HVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFSLNYP 676

Query: 666 SFTVTLGASQT---FKRTVTNVGDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYS 674
           S  V     +T     R VTNVG   SIYS  +KAP G+ V V P+ L F  V++ L+Y 
Sbjct: 677 SIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYR 736

BLAST of Tan0008814 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 523.5 bits (1347), Expect = 2.6e-148
Identity = 316/735 (42.99%), Postives = 425/735 (57.82%), Query Frame = 0

Query: 16  TYIVHVEQPELGISGDKIDLES-WYTSFLPDTITITTSNEQSRLLYSYRNVMSGFSARLT 75
           TYIVH+ + ++  S    DL S WY S      ++ + ++ + LLY+Y N + GFS RLT
Sbjct: 31  TYIVHMAKSQMPSS---FDLHSNWYDS------SLRSISDSAELLYTYENAIHGFSTRLT 90

Query: 76  EEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFG-LWKNSKFGEGVIIGVLDTG 135
           +E+  ++  + G IS  PE    LHTT TP +LGL++    L+  +     V++GVLDTG
Sbjct: 91  QEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTG 150

Query: 136 ITPDHPSFSDDGMPQPPAKWKGGCE----FDASI-------------------------- 195
           + P+  S+SD+G    P+ WKGGCE    F AS+                          
Sbjct: 151 VWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESK 210

Query: 196 -----------------TAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCSSS 255
                            TAAG+ VEGA  LG A G A GMAP A +A+YKVC   GC SS
Sbjct: 211 ESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSS 270

Query: 256 DILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSKSS 315
           DILAA+D AI D V+VLS+SLGG  S ++ D +AIG+FAA+++GI VSCSAGN+GPS SS
Sbjct: 271 DILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSS 330

Query: 316 LSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVYAGR----- 375
           LSN APWI TVGA   DR   ALA LGNG  ++G SLF+    P   LP +YAG      
Sbjct: 331 LSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNAT 390

Query: 376 -----------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTLAD 435
                            K+V+C++G    R+ KG VVK AGG  MIL N   +G   +AD
Sbjct: 391 NGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGVGMILANTAANGEELVAD 450

Query: 436 AHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGDTTFSPAMASFSSRGPSRFSS 495
           AH+LPAT V   AG  I+ Y+++  NP A+IS  GT++G    SP +A+FSSRGP+  + 
Sbjct: 451 AHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG-VKPSPVVAAFSSRGPNSITP 510

Query: 496 GILKPDITGPGVNILAAWPFPLDNN--TNTNTNSTFNVISGTSMSCPHLSGIAALIKNIH 555
            ILKPD+  PGVNILAAW          + +    FN+ISGTSMSCPH+SG+AAL+K++H
Sbjct: 511 NILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVH 570

Query: 556 PSWSPAAIKSAIMTSSDITNPRGQLIVD-QDLKPANFFAMGAGHVNPSRAADPGLVYDIQ 615
           P WSPAAI+SA+MT++  T   G+ ++D    KP+  F  GAGHV+P+ A +PGL+YD+ 
Sbjct: 571 PEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLT 630

Query: 616 PDDYIPYLCGL-YADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGASQTFK--RTV 674
            +DY+ +LC L Y   Q+  +  R  TC         DLNYPSF V +     +K  RTV
Sbjct: 631 TEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTV 690

BLAST of Tan0008814 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 504.6 bits (1298), Expect = 1.2e-142
Identity = 310/744 (41.67%), Postives = 422/744 (56.72%), Query Frame = 0

Query: 12  TKLQTYIVHVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLYSYRNVMSGFSA 71
           ++ QTY++H        +  K  + S + S   + I          + Y Y N MSGFSA
Sbjct: 39  SRKQTYVIHTV-----TTSTKHIVTSLFNSLQTENIN-DDDFSLPEIHYIYENAMSGFSA 98

Query: 72  RLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSKFGEGVIIGVLD 131
            LT++Q+  ++   GFISA P+ +L+LHTT++ ++LGL    GLW  +     VIIG++D
Sbjct: 99  TLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSDVIIGLVD 158

Query: 132 TGITPDHPSFSDDGMPQPPAKWKGGCE----FDAS------------------------- 191
           TGI+P+H SF D  M   P++W+G C+    F +S                         
Sbjct: 159 TGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYESIVGKINE 218

Query: 192 ------------------ITAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCSPKGCS 251
                              TAAG  V  A   G A+G A+GM   + +A YK C   GC+
Sbjct: 219 TTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKACWALGCA 278

Query: 252 SSDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGNSGPSK 311
           S+D++AA+D AI DGVDV+SLSLGG   PF++D IAI  F A+QK IFVSCSAGNSGP+ 
Sbjct: 279 STDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPTA 338

Query: 312 SSLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVY---AGR 371
           S++SN APW++TV AS  DR   A+ ++GN     G SL++     +  LPL +   AG 
Sbjct: 339 STVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGKSLKN--LPLAFNRTAGE 398

Query: 372 -------------------KVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGFSTL 431
                              K+V+C +G   GR AKG  VK +GGAAM+L++ + +G   L
Sbjct: 399 ESGAVFCIRDSLKRELVEGKIVICLRGAS-GRTAKGEEVKRSGGAAMLLVSTEAEGEELL 458

Query: 432 ADAHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGDTTFSPAMASFSSRGPSRF 491
           AD HVLPA  + ++ G  +  Y++ + N  A++ FRGT  G T  +P +A+FSSRGPS  
Sbjct: 459 ADPHVLPAVSLGFSDGKTLLNYLAGAANATASVRFRGTAYGAT--APMVAAFSSRGPSVA 518

Query: 492 SSGILKPDITGPGVNILAAW-PFPLDNNTNTNTNST-FNVISGTSMSCPHLSGIAALIKN 551
              I KPDI  PG+NILA W PF   +   ++     FN+ISGTSM+CPH+SGIAALIK+
Sbjct: 519 GPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHISGIAALIKS 578

Query: 552 IHPSWSPAAIKSAIMTSSDITNPRGQLIVDQDL----KPANFFAMGAGHVNPSRAADPGL 611
           +H  WSPA IKSAIMT++ IT+ R + I D+        A  FA GAG+V+P+RA DPGL
Sbjct: 579 VHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDPGL 638

Query: 612 VYDIQPDDYIPYLCGL-YADAQVSIIVHRRVTC-SGVTRIREGDLNYPSFTVTL--GA-- 671
           VYD    DY+ YLC L Y   ++ +      TC S    +  GDLNYPSF V L  GA  
Sbjct: 639 VYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDLNYPSFAVNLVNGANL 698

Query: 672 -SQTFKRTVTNVGDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVR 674
            +  +KRTVTNVG     Y   ++ P GV V V P+ LKF K  E+L+Y+VT+   +  R
Sbjct: 699 KTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKARERLSYTVTYDA-EASR 758

BLAST of Tan0008814 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 502.7 bits (1293), Expect = 4.7e-142
Identity = 313/757 (41.35%), Postives = 421/757 (55.61%), Query Frame = 0

Query: 9   SNPTKLQTYIVHVE---QPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLYSYRNV 68
           S+   L++YIVHV+   +P L  S +     +W+ S L    ++ +S + + LLYSY   
Sbjct: 25  SSSDGLESYIVHVQRSHKPSLFSSHN-----NWHVSLLR---SLPSSPQPATLLYSYSRA 84

Query: 69  MSGFSARLTEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSKFGEGV 128
           + GFSARL+  Q  A+      IS  P+    +HTTHTP +LG +Q  GLW NS +GE V
Sbjct: 85  VHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDV 144

Query: 129 IIGVLDTGITPDHPSFSDDGMPQPPAKWKGGCE----FDAS------------------- 188
           I+GVLDTGI P+HPSFSD G+   P+ WKG CE    F AS                   
Sbjct: 145 IVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQ 204

Query: 189 --------------------------ITAAGAFVEGAQALGNARGEAAGMAPLAHLAIYK 248
                                      TAAG+ V  A     ARG A GMA  A +A YK
Sbjct: 205 RNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYK 264

Query: 249 VCSPKGCSSSDILAALDAAIDDGVDVLSLSLG--GPPSPFFIDNIAIGSFAAIQKGIFVS 308
           +C   GC  SDILAA+D A+ DGV V+SLS+G  G    +  D+IAIG+F A + GI VS
Sbjct: 265 ICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVS 324

Query: 309 CSAGNSGPSKSSLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFL 368
           CSAGNSGP+  + +N APWILTVGAS  DR   A A  G+G V++G SL+     P + L
Sbjct: 325 CSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQL 384

Query: 369 PLVYAG------------------RKVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQP 428
            LVY+G                   K+V+C++GG   R+ KG  VK AGGA MIL N   
Sbjct: 385 SLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAE 444

Query: 429 DGFSTLADAHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGDTTFSPAMASFSS 488
            G    AD+H++PAT V   AG +I+ YI +S +P A ISF GT+IG +  SP +A+FSS
Sbjct: 445 SGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSS 504

Query: 489 RGPSRFSSGILKPDITGPGVNILAAW-----PFPLDNNTNTNTNSTFNVISGTSMSCPHL 548
           RGP+  +  ILKPD+  PGVNILA W     P  LD +        FN+ISGTSMSCPH+
Sbjct: 505 RGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR---RVQFNIISGTSMSCPHV 564

Query: 549 SGIAALIKNIHPSWSPAAIKSAIMTSSDITNPRGQLIVDQDL-KPANFFAMGAGHVNPSR 608
           SG+AAL++  HP WSPAAIKSA++T++      G+ I D    K +N F  GAGHV+P++
Sbjct: 565 SGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNK 624

Query: 609 AADPGLVYDIQPDDYIPYLCGLYADAQVSIIVHRRVT----CSGVTRIREGDLNYPSFTV 668
           A +PGLVYDI+  +Y+ +LC +  +    ++  +  T    C        GDLNYPSF+V
Sbjct: 625 ALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSV 684

Query: 669 ---TLGASQTFKRTVTNVGDAI-SIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTF 677
              + G    +KR V NVG  + ++Y   +K+P  V + V+P  L FSK    L Y VTF
Sbjct: 685 VFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTF 744

BLAST of Tan0008814 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 493.4 bits (1269), Expect = 2.9e-139
Identity = 303/748 (40.51%), Postives = 411/748 (54.95%), Query Frame = 0

Query: 12  TKLQTYIV---HVEQPELGISGDKIDLESWYTSFLPDTITITTSNEQSRLLYSYRNVMSG 71
           T  +TYI+   H ++PE  ++        WYTS L         N +S LLY+Y     G
Sbjct: 25  TAKKTYIIRVNHSDKPESFLTH-----HDWYTSQL---------NSESSLLYTYTTSFHG 84

Query: 72  FSARL-TEEQVKAMEEKDGFISAKPESILNLHTTHTPKYLGLNQQFGLWKNSKFGEGVII 131
           FSA L + E    +   +  +    + +  LHTT TP++LGLN +FG+        GVII
Sbjct: 85  FSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVII 144

Query: 132 GVLDTGITPDHPSFSDDGMPQPPAKWKGGCE----FDA---------------------- 191
           GVLDTG+ P+  SF D  MP+ P+KWKG CE    FD+                      
Sbjct: 145 GVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASG 204

Query: 192 ----------------------SITAAGAFVEGAQALGNARGEAAGMAPLAHLAIYKVCS 251
                                 S TAAG+ V  A  LG A G A GMA  A +A YKVC 
Sbjct: 205 GGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCW 264

Query: 252 PKGCSSSDILAALDAAIDDGVDVLSLSLGGPPSPFFIDNIAIGSFAAIQKGIFVSCSAGN 311
             GC  SDILAA+D AI DGVDVLSLSLGG  +P++ D IAIG+F+A+++G+FVSCSAGN
Sbjct: 265 STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGN 324

Query: 312 SGPSKSSLSNEAPWILTVGASVHDRRIVALAKLGNGAVYSGESLFQPMDFPSTFLPLVY- 371
           SGP+++S++N APW++TVGA   DR   A A LGNG   +G SL+  +   +  L LVY 
Sbjct: 325 SGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYN 384

Query: 372 -------------------AGRKVVVCEKGGGIGRIAKGLVVKNAGGAAMILINQQPDGF 431
                                 K+VVC++G    R+ KG VV++AGG  MI+ N    G 
Sbjct: 385 KGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGE 444

Query: 432 STLADAHVLPATHVSYTAGLRIKAYISSSQNPIATISFRGTIIGDTTFSPAMASFSSRGP 491
             +AD+H+LPA  V    G  ++ Y+ S   P A + F+GT++ D   SP +A+FSSRGP
Sbjct: 445 ELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGP 504

Query: 492 SRFSSGILKPDITGPGVNILAAW-----PFPLDNNTNTNTNSTFNVISGTSMSCPHLSGI 551
           +  +  ILKPD+ GPGVNILA W     P  LD ++     + FN++SGTSMSCPH+SG+
Sbjct: 505 NTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSR---RTQFNIMSGTSMSCPHISGL 564

Query: 552 AALIKNIHPSWSPAAIKSAIMTSSDI-TNPRGQLIVDQDLKPANFFAMGAGHVNPSRAAD 611
           A L+K  HP WSP+AIKSA+MT++ +  N    L    D   +N +A G+GHV+P +A  
Sbjct: 565 AGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALS 624

Query: 612 PGLVYDIQPDDYIPYLCGL--YADAQVSIIVHRRVTCSGVTRIREGDLNYPSFTVTLGAS 671
           PGLVYDI  ++YI +LC L    D  V+I+    V CS       G LNYPSF+V  G  
Sbjct: 625 PGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFS-DPGQLNYPSFSVLFGGK 684

Query: 672 QT--FKRTVTNVGDAISIYSPIIKAPLGVSVTVTPRNLKFSKVNEKLTYSVTFTRDDLVR 678
           +   + R VTNVG A S+Y   +     V ++V P  L F  V EK  Y+VTF     V 
Sbjct: 685 RVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVS 744